Consulta: creatorFacets:"Rodrigues,S.S."
Registros recuperados: 2
Data/hora: 11/06/2024 04:41:40

Comparison of the specificity of PCR and the histopathological detection of leishmania for the diagnosis of American cutaneous leishmaniasis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Medeiros,A.C.R.; Rodrigues,S.S.; Roselino,A.M.F.
More precise and rapid diagnostic methods for American cutaneous leishmaniasis (ACL) are necessary because of the growing number of cases observed in Brazil, including the northeastern region of the State of São Paulo. We applied PCR to 54 skin or mucosal biopsies from patients with a clinical and/or laboratory diagnosis of ACL using primers 13A and 13B, with positive results being obtained for 82% of the samples. When the PCR results were compared to those of histopathological leishmania detection, PCR showed superior results with 81.5% sensitivity and 95% CI of 68.0-95.1%. The Montenegro skin test (MST) was positive in 88.7% of patients. Since MST cannot be used as a diagnostic tool in endemic areas, the present results strongly suggest the use of PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Cutaneous leishmaniasis; PCR; Histopathological detection; Leishmania detection.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400002

Hepatitis C virus infection in a Brazilian population with sickle-cell anemia
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Torres,M.C.M.R.; Pereira,L.M.M.B.; Ximenes,R.A.A.; Araújo,A.S.; Secaf,M.; Rodrigues,S.S.; Bezerra,A.C.S.; Conceição,I.B.; Valença,M.I.B.; Martinelli,A.L.C.
Patients with sickle-cell anemia submitted to frequent blood transfusions are at risk of contamination with hepatitis C virus (HCV). Determination of HCV RNA and genotype characterization are parameters that are relevant for the treatment of the viral infection. The objective of the present study was to determine the frequency of HCV infection and the positivity for HCV RNA and to identify the HCV genotype in patients with sickle-cell anemia with a history of blood transfusion who had been treated at the Hospital of the HEMOPE Foundation. Sera from 291 patients were tested for anti-HCV antibodies by ELISA 3.0 and RIBA 3.0 Chiron and for the presence of HCV RNA by RT-PCR. HCV genotyping was performed in 19 serum samples. Forty-one of 291 patients (14.1%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C; Anti-HCV antibodies; HCV RNA; HCV genotype; Sickle-cell anemia.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300006