Consulta: creatorFacets:"Rogberg-Muñoz,A"
Registros recuperados: 3
Data/hora: 16/06/2024 16:58:36

D-loop mitochondrial genetic analysis in Abeerden angus old type from Argentina
Provedor de dados: 46
Autores: Villegas Castagnasso,E.E; Rogberg-Muñoz,A; Prando  ,A.J; Baldo,A; Giovambattista,G.
The massive use of reproductive breeding technologies (mainly Artificial Insemination and Embryo Transfer) resulted in an important decrease in the diversity in many breeds, and some genetic lines have been lost. Within the Angus breed, the &amp;#8220;New Type&amp;#8221; gain importance since 1970, leaving the &amp;#8220;Old Type&amp;#8221; to a reduced number of herds in the whole world. The objective of this work was to determine the genetic diversity in an &amp;#8220;Old Type&amp;#8221; herd in Argentina. DNA samples were analyzed for sequence variation in the hypervariable region of the mitochondrial DNA (D-loop). Sequence comparison and phylogenetic analyses revealed that haplotypes fell into European haplogroup (T3) in general, and in particular had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; Bos Taurus; Angus; Matrilineages origin; Genetic diversity.
Ano: 2015
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332015000300003

Development of typing methods based on pyrosequencing technology fot the analysis of six bovine genes related to marbling
Provedor de dados: 46
Autores: Ripoli,M.V; Rogberg-Muñoz,A; Liron,J.P; Giovambattista,G.
Several methods such as PCR-RFLP, OLA, DNA sequencing, PCR-SSCP and ARMS-PCR have been developed to detect the allelic variation at substitutions K232A of DGAT1, GH6.1 of GH, F279Y of GHR, R4C of LEP, I74V of FABP4, and at the transition in the TG 5´ leader sequence. Most of these methods are manual processes and therefore increase the time spent on the assay and limit the number of animals analyzed. Herein, we describe the development of pyrosequencing-based methods for the bovine DGAT1, GH, GHR, LEP, FABP4 and TG genes, whose polymorphisms have been associated with variation in carcass composition. This method was validated by analyzing DNA samples belonging to the Aberdeen Angus and Hereford breeds previously typed by PCR-SSCP, PCR-RFLP and/or DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine molecular markers; Polymorphism; Pyrosequencing; Marbling.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332013000300005

Evaluation of six nucleotide polymorphisms for bovine traceability in the context of the Argentine-Chinese beef trade
Provedor de dados: 46
Autores: Ripoli,M.V; Wei,S; Rogberg-Muñoz,A; Guo,B.L; Goszczynski,D.E; Fernandez,M.E; Mellucci,L; Lirón,J.P; Villarreal,E; Wei,Y.M; Giovambattista,G.
Genetic traceability refers to methods associated with the identification of animals and their products through DNA characterization of individuals, breeds or species. To trace breeds, it is necessary to define the breed groups to analyze, and the most appropriate molecular marker set. The selection of genetic markers depends on the gene frequency distribution, the genetic distance among breeds and the presence of private alleles. In this study, we assessed six single nucleotide polymorphisms (SNPs) located in the DGAT1, TG, LEP, GH, FABP4 and GnRHR genes, as potential genetic markers to be included into a panel for genetic traceability for the identification of breed origin associated with the bovine beef trade. The results of the genetic characterization...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breed traceability; Genetic traceability; Single nucleotide polymorphism; Chinese cattle; Argentine cattle.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332013000300004