Consulta: creatorFacets:"Ruvolo-Takasusuki,Maria Claudia C."
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Data/hora: 09/06/2024 07:24:30

Esterase-3 polymorphism in the sugarcane borer Diatraea saccharalis (Lepidoptera, Pyralidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Ruvolo-Takasusuki,Maria Claudia C.; Machado,Maria de Fátima P.S.; Conte,Hélio.
The migration rate of esterases and their substrate specificity for 4-methylumbelliferyl esters (acetate, propionate, and butyrate) and alpha- and beta-naphthyl esters were analyzed in Diatraea saccharalis by starch gel electrophoresis. Substrate preference of esterases was observed with Est-2 and Est-8 isozymes showing substrate specificity for 4-methylumbelliferyl esters and Est-4 isozyme showing specificity for 4-methylumbelliferyl butyrate and alpha-naphthyl butyrate. Allele variation was detected at the Est-3 locus. Two alleles, Est-3F and Est-3S, were identified in pupae with fluorogenic and ester-naphthyl substrates. Chi-square analysis showed no differences between the observed genotypic frequencies and those expected on the basis of Hardy-Weinberg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esterase; Genetic variability; Isozymes; Moth; Sugarcane borer.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100012