Consulta: creatorFacets:"SOARES,A. M."
Registros recuperados: 2
Data/hora: 17/06/2024 04:32:34

INTRASPECIFIC VARIATION IN NEUROTOXIC AND MYOTOXIC ACTIVITIES OF Bothrops neuwiedii VENOMS
Provedor de dados: Journal of Venomous Animals and Toxins
Autores: BORJA-OLIVEIRA,C. R.; SOARES,A. M.; ZAMUNÉR,S. R.; HYSLOP,S.; GIGLIO,J. R.; PRADO-FRANCESCHI,J.; RODRIGUES-SIMIONI,L.
Snake venoms frequently vary in composition. In this work, we compared the neurotoxic and myotoxic activities of 16 lots of Bothrops neuwiedii venoms from different regions of Brazil, using chick biventer cervicis preparations. The neuromuscular blockade varied from 2% to 100% after 120 min incubation with venoms (50 mug/ml). In all cases, this blockade was irreversible and concentration-dependent; at low concentrations (10-20 mug/ml), 15 of the 16 venom lots failed to abolish responses to acetylcholine (110 muM), but blocked responses to KCl (13.4 muM), and induced contracture. At 5-20 mug/ml, the most active venom totally blocked twitch-tension without affecting responses to acetylcholine and KCl. Polyacrylamide gel electrophoresis for basic proteins...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops neuwiedii venom; Intraspecific variation; Myotoxicity; Neurotoxicity; Neuromuscular blockade.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100008

POLYACRYLAMIDE GEL ELECTROPHORESIS AS A TOOL FOR THE TAXONOMIC IDENTIFICATION OF SNAKES FROM THE ELAPIDAE AND VIPERIDAE FAMILIES
Provedor de dados: Journal of Venomous Animals and Toxins
Autores: SOARES,A. M.; ANZALONI PEDROSA,L. H.; FONTES,M. R. M.; DA SILVA,R. J.; GIGLIO,J. R.
Polyacrylamide gel electrophoresis (PAGE) for basic proteins may be a useful tool for the characterization of whole snake venoms and for the taxonomic classification of snakes of the Elapidae and Viperidae families. However, due to the close proximity of molecular weights among toxins bearing different net charges, sodium dodecyl sulfate (SDS) - PAGE was not able to provide an efficient differentiation. This article reports the electrophoretic analysis of several venoms from the genera Micrurus, Bothrops, Bothriopsis, Crotalus and Lachesis and shows a typical and distinctive electrophoretic profile for each species, with intraspecific and geographic variations. Even in cases in which extreme morphological similarities were present, such as between B....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Snake venoms; Basic toxins; Polyacrylamide gel electrophoresis; Elapidae; Viperidae.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301998000200005