Consulta: creatorFacets:"Salgado,Hérida Regina Nunes"
Registros recuperados: 12
Data/hora: 10/06/2024 07:59:33

Development and evaluation of an emulsion containing lycopene for combating acceleration of skin aging
Provedor de dados: 60
Autores: Cefali,Letícia Caramori; Souza-Moreira,Tatiana Maria; Corrêa,Marcos Antônio; Salgado,Hérida Regina Nunes; Isaac,Vera Lucia Borges.
Lycopene, a carotenoid and potent antioxidant is found in large quantities in tomatoes. Lycopene combats diseases, such as cardiovascular disease and different types of cancer, including prostate cancer. However, its topical use in emulsion form for the combat of skin aging is under-explored. The aim of the present study was to develop an emulsion containing lycopene extracted from salad tomatoes and evaluate its cytotoxicity, stability, rheological behavior, antioxidant activity and phytocosmetic permeation. The developed cosmetic comprised an oil phase made up of shea derivatives and was evaluated in terms of its physiochemical stability, spreadability, thermal analysis, rheological behavior, microbiological quality, cytotoxicity, antioxidant activity,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carotenoid/antioxidant activity; Lycopene/emulsion form/cytotoxicity; Lycopene/emulsion form/permeation; Lycopene/emulsion form/rheology; Lycopene/emulsion form/thermal analyses; Phytocosmetics; Skin aging/treatment.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300579

Stability study of azithromycin in ophthalmic preparations
Provedor de dados: 60
Autores: Moreno,Andréia de Haro; Silva,Maria Fernanda Conz da; Salgado,Hérida Regina Nunes.
A stability study of azithromycin in ophthalmic preparations was developed by submission to different types of light, temperature and pH, using the biodiffusion assay (cylinder 3 x 3) for the quantifications. Bacillus subtilis, ATCC 9372, was used as test organism. The used concentration range was of 50 to 200 µg/mL. The study demonstrated that the drug suffered degradation when submitted to the ultraviolet light, germicide light, solar luminosity, acid solution, basic solution and hydrogen peroxide solution. The results were analyzed by the analysis of variance (ANOVA).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Azithromycin/stability study; Bioassay; Photodegradation; Ophthalmic preparations.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200005

Development and validation of a microbiological assay for determination of chlorhexidine digluconate in aqueous solution
Provedor de dados: 60
Autores: Fiorentino,Flávia Angélica Másquio; Corrêa,Marcos Antonio; Salgado,Hérida Regina Nunes.
Chlorhexidine (CHX) is a broad-spectrum antiseptic that is used in many topical pharmaceutical formulations. Because there is no official microbiological assay reported in the literature that is used to quantify CHX, this paper reports the development and validation of a simple, sensitive, accurate and reproducible agar diffusion method for the dosage of chlorhexidine digluconate (CHX-D) in an aqueous solution. The assay is based on the inhibitory effect of CHX-D upon the strain of Staphylococcus aureus ATCC 25923, which is used as the test microorganism. The design 3x3 parallel-line model was used. The results were treated statistically by analysis of variance (ANOVA), and they were excellent in terms of linearity (r = 0.9999), presenting a significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agar diffusion; Antiseptic; Chlorhexidine; Microbiological assay; Quality control; Validation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200017

Development and validation of UV spectrophotometric method for orbifloxacin assay and dissolution studies
Provedor de dados: 60
Autores: Cazedey,Edith Cristina Laignier; Salgado,Hérida Regina Nunes.
New, simple and cost effective UV-spectrophotometric method was developed for the estimation of orbifloxacin in pharmaceutical formulation. Orbifloxacin was estimated at 290 nm in 0.5 M hydrochloric acid. Linearity range was found to be 1.0-6.0 μg mL-1. The method was tested and validated for various parameters according to main guidelines. The proposed method was successfully applied for the determination of orbifloxacin in tablets. The results demonstrated that the procedure is accurate, precise and reproducible, while being simple, economical and less time consuming. It can be suitably applied for the estimation of orbifloxacin in routine quality control and dissolution studies.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicines/quality control; Medicines/dissolution; Orbifloxacin/determination; UV spectrophotometry/quantitative analysis/validation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300457

Total polyphenols from Syzygium cumini (L.) Skeels fruit extract
Provedor de dados: 60
Autores: Migliato,Ketylin Fernanda; Carvalho,Elisângela Simões de; Sacramento,Luis Vitor Silva do; Mello,João Carlos Palazzo de; Baby,André Rolim; Velasco,Maria Valéria Robles; Salgado,Hérida Regina Nunes.
A precise, accurate and low cost spectrophotometric method was developed and validated for routine determination of total polyphenols, as pyrogallic acid equivalents, from the percolated and lyophilized extract of Syzygium cumini (L.) Skeels fruits. Validation was assessed experimentally and data were rigorously treated by statistical analysis. Analytical parameters were: linearity, interval (range), precision and recovery/accuracy, limit of detection (LOD, μg mL-1) and limit of quantification (LOQ, μg mL-1). The visible spectrophotometric method presented linearity (r² = 0.9979 ± 0.0010) over the concentration range 0.25-7.5 μg mL-1 of standard pyrogallic acid, precision &lt; 2.918171%, recovery/accuracy ranging from 96.228693 to 107.17701%, LOD = 0.21 μg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Total polyphenols; Syzygium cumini; Botanical extract; Analytical method validation; Spectrophotometry.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100015

Stability-indicating RP-LC method for quantification of fusidic acid in cream
Provedor de dados: 60
Autores: Curbete,Mariane Machado; Salgado,Hérida Regina Nunes.
ABSTRACT Fusidic acid is an antibiotic steroid indicated for the treatment of infections caused by the genus Staphylococcus, including methicillin resistant Staphylococcus aureus strains, and other Gram-positive bacteria. In the present study, a stability-indicating reversed-phase liquid chromatography (RP-LC) method was developed and validated for the determination of fusidic acid in dermatological cream as an alternative to existing methods. Analyses were performed using a C18 column and guard column at room temperature, eluting with an isocratic mobile phase of acetonitrile and water (72:28, v/v), adjusted to pH 3.5 with acetic acid, pumped at a flow rate of 1.0 mL min-1, detection at 210 nm and 20 µL of injection volume. The forced degradation study...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fusidic acid/stability-indicating/quality control/validation; Chromatography/reversed-phase.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300447

Development and validation of a rapid turbidimetric assay to determine the potency of norfloxacin in tablets
Provedor de dados: 60
Autores: Chierentin,Lucas; Salgado,Hérida Regina Nunes.
Norfloxacin is one of the first commercially available (and most widely used) fluoroquinolone antibiotics. This paper reports the development and validation of a simple, sensitive, accurate and reproducible turbidimetric assay method to quantify norfloxacin in tablets formulations in only 4 hours. The bioassay is based on the inhibitory effect of norfloxacin upon the strain ofStaphylococcus epidermidis ATCC 12228 IAL 2150 used as test microorganism. The assay was performed 3x3 parallel lines like, three tubes for each concentration of reference substance and three tubes for each sample concentration. The results were treated statistically by analysis of variance and were found to be linear (r2 = 0.9999) in the selected range of 25-100 μg mL-1; precise...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbiological assay; Quality control; Norfloxacin/quantification in tablets; Turbidimetric method/quality control/validation; Antimicrobials/quality control.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300629

Corrositex®, BCOP and HET-CAM as alternative methods to animal experimentation
Provedor de dados: 60
Autores: Cazedey,Edith Cristina Laignier; Carvalho,Flávia Chiva; Fiorentino,Flávia Angélica Másquio; Gremião,Maria Palmira Daflon; Salgado,Hérida Regina Nunes.
Tests in animals are used as models in toxicological and investigative studies. However, such tests have been considered inhumane because they can cause pain and suffering to experimental animals, while these methods can often be subjective. Protests calling for animal protection have questioned the effectiveness of in vivo tests and suggest the introduction of alternative, in vitro methods. International organizations, such as the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), the National Institute of Health (NIH), the Organization for Economic Co-operation and Development (OECD), that regulate and develop new alternative animal models, have indicated the running of preliminary assays and execution of sequential...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal experimentation/alternative methods in vitro; Experiments/in vitro methods; Corrositex®/in vitro method/experiments; BCOP/in vitro method/experiments; HET-CAM/in vitro method/experiments.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400021

Design of antiseptic formulations containing extract of Plinia cauliflora
Provedor de dados: 60
Autores: Oliveira,Lara Alexandre de; Souza-Moreira,Tatiana Maria de; Cefali,Letícia Caramori; Chiari,Bruna Galdorfini; Corrêa,Marcos Antonio; Isaac,Vera Lucia Borges; Salgado,Hérida Regina Nunes; Pietro,Rosemeire Cristina Linhari Rodrigues.
The leaves of the Brazilian species Plinia cauliflora were used to obtain active hydroalcoholic extract and fractions enabling the development of efficient antiseptic pharmaceutical formulations. A chemical composition of 70% ethanol extract, aqueous and ethyl acetate fractions was analyzed by thin-layer chromatography and for phenol content. Antimicrobial activity was tested against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Lactobacillus acidophilus and Candida albicans by the agar diffusion method and the minimum inhibitory concentration was assayed by broth microdilution. Extract microbiological quality was tested to avoid contamination in the formulations. A mouthwash and a topical cream containing the extract were developed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antiseptic formulations/design; Plinia cauliflora/antimicrobial activity; Plinia cauliflora/antiseptic activity; Mouthwash; Plant extract; Topical cream.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300010

Validation of analytical methodology for quantification of cefazolin sodium pharmaceutical dosage form by high performance liquid chromatography to be applied for quality control in pharmaceutical industry
Provedor de dados: 60
Autores: Pedroso,Tahisa Marcela; Salgado,Hérida Regina Nunes.
A reversed-phase high performance liquid chromatography method was validated for the determination of cefazolin sodium in lyophilized powder for solution for injection to be applied for quality control in pharmaceutical industry. The liquid chromatography method was conducted on a Zorbax Eclipse Plus C18 column (250 x 4.6 mm, 5 μm), maintained at room temperature. The mobile phase consisted of purified water: acetonitrile (60: 40 v/v), adjusted to pH 8 with triethylamine. The flow rate was of 0.5 mL min-1 and effluents were monitored at 270 nm. The retention time for cefazolin sodium was 3.6 min. The method proved to be linear (r2=0.9999) over the concentration range of 30-80 µg mL-1. The selectivity of the method was proven through degradation studies....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cefazolin sodium/determination; High performance liquid chromatography/reverse-phase/quantitative analysis; Pharmaceutical industry/quality control; Cefalosporine/quality control; Medicines/quality control.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100022

A green approach for the quantification of daptomycin in pharmaceutical formulation by UV spectrophotometry
Provedor de dados: 60
Autores: Tótoli,Eliane Gandolpho; Salgado,Hérida Regina Nunes.
abstract Daptomycin is the first approved drug from a new class of antimicrobials, the cyclic lipopeptides, and is a very important antimicrobial agent in current clinical practice. Currently, there are no "green" analytical methods described in the literature to analyze the typical pharmaceutical dosage form of daptomycin. Thus, the aim of this work was to validate an environment-friendly spectrophotometric method in the UV region, for the analysis of daptomycin as a lyophilized powder. Water was used as diluent and the analyses were carried out on a spectrophotometer at 221 nm. The method met all validation requirements of the ICH guidelines, over a concentration range of 6-21 µg mL-1. A Student's t-test demonstrated that the proposed method was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobials/quality control; Daptomycin/determination; Spectrophotometry/UV region/quantitative analysis; Spectrophotometry/green analytical method.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400811

Stability-indicating capillary zone electrophoresis assay for the analysis of linezolid in tablets
Provedor de dados: 60
Autores: Oliveira,Cristiani Lopes Capistrano Gonçalves de; Salgado,Hérida Regina Nunes; Moraes,Maria de Lourdes Leite.
A simple and fast alternative methodology using capillary zone electrophoresis (CZE) to analyze linezolid and its cationic photodegradation products in tablets has been developed. The separation was carried out on fused silica capillary and conducted using 100 mM formic acid (pH 3.0) and by applying 30 KV voltage. Detection was performed at UV 254 nm. The optimized method was validated in terms of linearity, limits of detection and quantification, precision (repeatability), stability studies (selectivity) and accuracy. Good linearity (8-20 mg L-1) was obtained and the limit of detection was 0.95 mg L-1. The greatest advantages of the CZE method were the rapid set-up of instrumentation and capillary equilibration, short analysis time (12 min), low running...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capillary-electrophoresis/assay; Linezolid/tablets/stability; Pharmaceutical-drugs.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400621