Consulta: creatorFacets:"Sant'Ana,Débora de Mello Gonçales"
Registros recuperados: 6
Data/hora: 10/06/2024 02:23:25

Quantitative Study of the Myenteric Plexus of the Descending Colon of Young Rats Subjected to Intense Protein Deficiency
Provedor de dados: International Journal of Morphology
Autores: Araújo,Eduardo José de Almeida; Sant'Ana,Débora de Mello Gonçales; Molinari,Sonia Lucy; Miranda Neto,Marcílio Hübner de.
We studied the effects of a severely hypoproteic diet on the quantitative aspects of the myenteric plexus of the descending colon of young rats. Eighteen rats were divided into two groups, one of them being fed with a chow having 26% protein (control) and the other with a chow having 4% protein, balanced for minerals and vitamins, during 12 weeks. The whole-mounts of the descending colon had their myenteric neurons stained either with Giemsa or NADPH diaphorase. The rats from the experimental group had deficits of body weight (54.23%) and area of the descending colon (48.14%); additionally, we observed that there was no alteration in the total number of neurons of the colon, but a decrease in the number of NADPH-diaphorase positive neurons (37.80%). The...
Tipo: Journal article
Palavras-chave: Myenteric plexus; Protein-energy; Malnutrition (PEM); Descending colon.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500013

Atrophy of the Nitrergic Myenteric Neurons in the Descending Colon Rats Submitted to Protein and Vitamin Deficiency
Provedor de dados: International Journal of Morphology
Autores: Araújo,Eduardo José de Almeida; Hermes,Catchia; Miranda Neto,Marcílio Hubner de; Almeida,Elton Carlos de; Sant'Ana,Débora de Mello Gonçales.
Effects of protein and B-complex vitamin deficiency were assessed with respect to the morphometry of myenteric neurons in the descending colon of adult rats. Sixteen animals were divided into two groups: Control Group (CG, n=8) and Experimental Group (EG, n=8). The CG received 22% protein chow and the EG received 4% protein chow for 120 days. The descending colon was submitted to NADH- and NADPH-diaphorase technique in order to evidence nervous cells in the whole mounts preparations. In the EG, NADH-d positive neurons presented reduced nuclei, while NADPH-d positive neurons showed atrophy of the soma area (~41.7%) inducing an increase of the proportion occupied by the nucleus inside in the soma of these cells.
Tipo: Journal article
Palavras-chave: Malnutrition; Large intestine; Enteric nervous system; Myenteric plexus; Neural plasticity.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000300047

Intestinal Wall Atrophy and Increase of Sulphomucin Secretion in the Jejunal Epithelium of Rats Submitted to Severe Protein Malnutrition
Provedor de dados: International Journal of Morphology
Autores: Franco,Cássia Luiza de Marins; Sant'Ana,Débora de Mello Gonçales; Araújo,Eduardo José de Almeida.
The effects of severe protein malnutrition on the morphometry of the intestinal wall in the jejunum of adult rats were evaluated in this study. Six 90-day-old Wistar rats (Rattus norvegicus) were assigned into two groups: CG (Control Group, n = 3) and MG (Malnourished Group, n = 3). CG animals were fed a 26%-protein chow whereas controls were fed a 4%-protein chow. After 90 days, part of the jejunum was collected and subjected to routine histology. HE-staining and histochemical techniques - Periodic Acid Schiff (PAS) and Alcian Blue (AB), pH 2.5/pH 1.0 - were used for the detection of glycoconjugates in 4µm -thick transverse cuts. Morphometric analysis of the HE-stained cuts revealed a decrease of the total thickness of the jejunal wall, mostly on the...
Tipo: Journal article
Palavras-chave: Protein-energetic malnutrition; Small intestine; Morphometry; Mucins.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200026

Toxoplasma gondii Induces Death of Gastric Myenteric Neurons in Rats
Provedor de dados: International Journal of Morphology
Autores: Alves,Marcelo Sardeto; Silva,Aristeu Vieira da; Bianchi,Larissa Renata de Oliveira; Araújo,Eduardo José de Almeida; Sant'Ana,Débora de Mello Gonçales.
The aim of the study was quantifying and morphologically analyzing the myenteric neurons of the small and large gastric curvatures of the glandular stomach of rats infected the tachyzoites of the Toxoplasma gondii for 30 days. Ten male rats were assigned into two groups: Control Group (CG) and Experimental Group (EG). The animals from the CG received saline solution orally whereas the EG animals received 104 tachyzoites of the T. gondii genotype III strain (BTU II). After 30 days, euthanasia was conducted for the removal of the stomach, which was dissected under the stereomicroscope for removal of the tunica mucosa and the tela submucosa. Whole mounts were stained with Giemsa. Quantification of the myenteric neurons was conducted by using a 40X-objective...
Tipo: Journal article
Palavras-chave: Stomach; Toxoplasmosis; Enteric nervous system; Myenteric plexus.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000100048

Morfometría de la Pared Intestinal del Colon Ascendente de Ratas Sometidas a Desnutrición Proteica Severa
Provedor de dados: International Journal of Morphology
Autores: Hermes,Catchia; Azevedo,Jorge Fernandes de; Araújo,Eduardo José de Almeida; Sant'Ana,Débora de Mello Gonçales.
This paper aims to verify the effects of severe protein malnutrition over the intestinal ascending colon morphometrics in adult rats. 12 rats (90 days old) were divided into 2 groups: control (n = 5) and malnutritioned (n = 7). In the following 90 days, the rats of the control group received a 24% protein chow as the malnourished group received 4% protein chow. The animals were submitted to euthanasia according to the anesthetic protocol. Colon segments were collected and submitted to routine histological processing. The cuts were stained with HE and histochemical techniques for mucines. The morphometric analyses showed the sustenance of the whole wall and muscle tunic thickness, as well as the reduction of the thickness of the mucosa tunic, the amount of...
Tipo: Journal article
Palavras-chave: Hypoproteic Diet; Intestine; Intestinal Wall.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000100001

Análise morfométrica da parede intestinal e dinâmica de mucinas secretadas no íleo de frangos infectados por Toxoplasma gondii
Provedor de dados: Ciência Rural
Autores: Shiraishi,Celina Sayuri; Azevedo,Jorge Fernandes de; Silva,Aristeu Vieira da; Sant'Ana,Débora de Mello Gonçales; Araújo,Eduardo José de Almeida.
Objetivou-se analisar os efeitos da toxoplasmose sobre a morfometria da parede intestinal e a dinâmica de mucinas secretadas no íleo de frangos. Foram utilizados 16 frangos de corte machos (Gallus gallus) da linhagem Cobb, com 26 dias de idade. As aves foram divididas aleatoriamente em três grupos (G1, G2 e G3). O G1 não recebeu nenhum inóculo caracterizando o grupo controle, o G2 foi inoculado com cistos teciduais da cepa ME49 de Toxoplasma gondii, e o G3 foi inoculado com oocistos da cepa M7741 de T. gondii. Após 60 dias da inoculação, os animais foram mortos, e coletou-se o íleo, o qual foi submetido à rotina de processamento histológico. Cortes transversais de 4mm foram corados com Hematoxilina-Eosina (HE), Periodic Acid Schiff (PAS), Alcian Blue pH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasmose; Gallus gallus; Intestino delgado; Histologia; Células caliciformes; Morfometria.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000700030