Consulta: creatorFacets:"Santos,M.G.L."
Registros recuperados: 1
Data/hora: 16/06/2024 09:03:43

Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora
Provedor de dados: 56
Autores: Silva,T.M.A.; Alves,L.G.; Queiroz,K.C.S. de; Santos,M.G.L.; Marques,C.T.; Chavante,S.F.; Rocha,H.A.O.; Leite,E.L.
The brown algae Padina gymnospora contain different fucans. Powdered algae were submitted to proteolysis with the proteolytic enzyme maxataze. The first extract of the algae was constituted of polysaccharides contaminated with lipids, phenols, etc. Fractionation of the fucans with increasing concentrations of acetone produced fractions with different proportions of fucose, xylose, uronic acid, galactose, and sulfate. One of the fractions, precipitated with 50% acetone (v/v), contained an 18-kDa heterofucan (PF1), which was further purified by gel-permeation chromatography on Sephadex G-75 using 0.2 M acetic acid as eluent and characterized by agarose gel electrophoresis in 0.05 M 1,3 diaminopropane/acetate buffer at pH 9.0, methylation and nuclear magnetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fucan; Anticoagulant activity; Sulfated polysaccharides; Brown algae; Padina gymnospora.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400005