Consulta: creatorFacets:"Sarti,M.S.M.V."
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Data/hora: 11/06/2024 00:33:32

Biological activity and binding of estradiol to SK-Mel 23 human melanoma cells
Provedor de dados: 56
Autores: Sarti,M.S.M.V.; Visconti,M.A.; Castrucci,A.M.L.
Patients expressing estradiol receptors in melanoma cells have been reported to have a better prognosis. We therefore decided to investigate the in vitro effects of ß-estradiol and tamoxifen on the growth and tyrosinase activity of SK-Mel 23 human melanoma cells. Twenty-four-hour treatment with 0.4 nM ß-estradiol inhibited cell proliferation in 30% (0.70 ± 0.03 x 10(5) cells) and increased tyrosinase activity in 50% (7130.5 ± 376.5 cpm/10(5) cells), as compared to untreated cells (1.0 ± 0.05 x 10(5) cells and 4769 ± 25.5 cpm/10(5) cells, respectively). Both responses were completely (100%) blocked by 1 µM tamoxifen. Higher concentrations (up to 1.6 nM) or longer treatments (up to 72 h) did not result in a larger effect of the hormone on proliferation or...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: SK-Mel 23 cell line; Human melanoma; SS-estradiol; Tamoxifen; Cell proliferation; Tyrosinase activity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600016