Consulta: creatorFacets:"Sbragia,L."
Registros recuperados: 7
Data/hora: 02/06/2024 09:09:24

Brain caspase-3 and intestinal FABP responses in preterm and term rats submitted to birth asphyxia
Provedor de dados: 56
Autores: Figueira,R.L.; Gonçalves,F.L.; Simões,A.L.; Bernardino,C.A.; Lopes,L.S.; Castro e Silva,O.; Sbragia,L.
Neonatal asphyxia can cause irreversible injury of multiple organs resulting in hypoxic-ischemic encephalopathy and necrotizing enterocolitis (NEC). This injury is dependent on time, severity, and gestational age, once the preterm babies need ventilator support. Our aim was to assess the different brain and intestinal effects of ischemia and reperfusion in neonate rats after birth anoxia and mechanical ventilation. Preterm and term neonates were divided into 8 subgroups (n=12/group): 1) preterm control (PTC), 2) preterm ventilated (PTV), 3) preterm asphyxiated (PTA), 4) preterm asphyxiated and ventilated (PTAV), 5) term control (TC), 6) term ventilated (TV), 7) term asphyxiated (TA), and 8) term asphyxiated and ventilated (TAV). We measured body, brain,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neonatal asphyxia; Necrotizing enterocolitis; I-FABP; Caspase-3; Brain injury; Intestinal damage.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700703

Ventilation-induced changes correlate to pulmonary vascular response and VEGF, VEGFR-1/2, and eNOS expression in the rat model of postnatal hypoxia
Provedor de dados: 56
Autores: Figueira,R.L.; Gonçalves,F.L.; Prado,A.R.; Ribeiro,M.C.; Costa,K.M.; Silva,O. Castro e; Sbragia,L.
Neonatal asphyxia occurs due to reduction in oxygen supply to vital organs in the newborn. Rapid restoration of oxygen to the lungs after a long period of asphyxia can cause lung injury and decline of respiratory function, which result from the activity of molecules that induce vascular changes in the lung such as nitric oxide (NO) and vascular endothelial growth factors (VEGF). In this study, we evaluated the pulmonary and vascular morphometry of rats submitted to the model of neonatal asphyxia and mechanical ventilation, their expression of pulmonary VEGF, VEGF receptors (VEGFR-1/VEGFR-2), and endothelial NO synthase (eNOS). Neonate Sprague-Dawley rats (CEUA #043/2011) were divided into four groups (n=8 each): control (C), control submitted to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neonatal hypoxia; Mechanical ventilation; VEGF; VEGF receptors; ENOS enzyme.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100608

Maternal administration of cannabidiol promotes an anti-inflammatory effect on the intestinal wall in a gastroschisis rat model
Provedor de dados: 56
Autores: Callejas,G.H.; Figueira,R.L.; Gonçalves,F.L.L.; Volpe,F.A.P.; Zuardi,A.W.; Crippa,J.A.; Hallak,J.E.; Sbragia,L.
Gastroschisis (GS) is an abdominal wall defect that results in histological and morphological changes leading to intestinal motility perturbation and impaired absorption of nutrients. Due to its anti-inflammatory, antioxidant, and neuroprotective effects, cannabidiol (CBD) has been used as a therapeutic agent in many diseases. Our aim was to test the effect of maternal CBD in the intestine of an experimental model of GS. Pregnant rats were treated over 3 days with CBD (30 mg/kg) after the surgical induction of GS (day 18.5 of gestation) and compared to controls. Fetuses were divided into 4 groups: 1) control (C); 2) C+CBD (CCBD); 3) gastroschisis (G), and 4) G+CBD (GCBD). On day 21.5 of gestation, the fetuses were harvested and evaluated for: a) body...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastroschisis; Experimental model; Prenatal treatment; Cannabidiol.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500607

Inflammatory response in a rat model of gastroschisis is associated with an increase of NF-kappaB
Provedor de dados: 56
Autores: Sbragia,L.; Schmidt,A.F.; Moraes,S.; Bittencourt,D.G.; Gonçalves,F.L.L.; Pereira,L.A.V.D.; Velloso,L.A.
Babies with gastroschisis have high morbidity, which is associated with inflammatory bowel injury caused by exposure to amniotic fluid. The objective of this study was to identify components of the inflammatory response in the intestine and liver in an experimental model of gastroschisis in rats. The model was surgically created at 18.5 days of gestation. The fetuses were exposed through a hysterotomy and an incision at the right of the umbilicus was made, exposing the fetal bowel. Then, the fetus was placed back into the uterus until term. The bowel in this model had macro- and microscopic characteristics similar to those observed in gastroschisis. The study was conducted on three groups of 20 fetuses each: gastroschisis, control, and sham fetuses. Fetal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastroschisis; Inflammation; Inflammatory mediators; Nuclear factor kappaB.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200006

VEGF receptor expression decreases during lung development in congenital diaphragmatic hernia induced by nitrofen
Provedor de dados: 56
Autores: Sbragia,L.; Nassr,A.C.C.; Gonçalves,F.L.L.; Schmidt,A.F.; Zuliani,C.C.; Garcia,P.V.; Gallindo,R.M.; Pereira,L.A.V.
Changes in vascular endothelial growth factor (VEGF) in pulmonary vessels have been described in congenital diaphragmatic hernia (CDH) and may contribute to the development of pulmonary hypoplasia and hypertension; however, how the expression of VEGF receptors changes during fetal lung development in CDH is not understood. The aim of this study was to compare morphological evolution with expression of VEGF receptors, VEGFR1 (Flt-1) and VEGFR2 (Flk-1), in pseudoglandular, canalicular, and saccular stages of lung development in normal rat fetuses and in fetuses with CDH. Pregnant rats were divided into four groups (n=20 fetuses each) of four different gestational days (GD) 18.5, 19.5, 20.5, 21.5: external control (EC), exposed to olive oil (OO), exposed to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Congenital diaphragmatic hernia; VEGFR; Rat model; Nitrofen; Lung development.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200171

Early neonatal echocardiographic findings in an experimental rabbit model of congenital diaphragmatic hernia
Provedor de dados: 56
Autores: Manso,P.H.; Figueira,R.L.; Prado,C.M.; Gonçalves,F.L.; Simões,A.L.B.; Ramos,S.G.; Sbragia,L.
This study aimed to demonstrate that congenital diaphragmatic hernia (CDH) results in vascular abnormalities that are directly associated with the severity of pulmonary hypoplasia and hypertension. These events increase right ventricle (RV) afterload and may adversely affect disease management and patient survival. Our objective was to investigate cardiac function, specifically right ventricular changes, immediately after birth and relate them to myocardial histological findings in a CDH model. Pregnant New Zealand rabbits underwent the surgical procedure at 25 days of gestation (n=14). CDH was created in one fetus per horn (n=16), and the other fetuses were used as controls (n=20). At term (30 days), fetuses were removed, immediately dried and weighed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Congenital diaphragmatic hernia; Right ventricle; Pulmonary arterial hypertension; Echocardiography.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300234

Contractile profile of esophageal and gastric fundus strips in experimental doxorubicin-induced esophageal atresia
Provedor de dados: 56
Autores: Capeto,F.A.; Lima,F.J.B.; Okoba,W.; Ramos,F.L.; Messias,T.F.A.; Rigonatto,G.A.; Sbragia,L.; Magalhães,P.J.C.; Melo-Filho,A.A.
Esophageal atresia (EA) is characterized by esophageal and gastric motility changes secondary to developmental and postsurgical damage. This study evaluated the in vitro contractile profile of the distal esophagus and gastric fundus in an experimental model of EA induced by doxorubicin (DOXO). Wistar pregnant rats received DOXO 2.2 mg/kg on the 8th and 9th gestational days. On day 21.5, fetuses were collected, sacrificed, and divided into groups: control, DOXO without EA (DOXO-EA), and DOXO with EA (DOXO+EA). Strips from the distal esophagus and gastric fundus were mounted on a wire myograph and isolated organ-bath system, respectively, and subjected to increasing concentrations of carbamylcholine chloride (carbachol, CCh). The isolated esophagus was also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esophageal atresia; Doxorubicin; Experimental; Esophageal contractility.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500458