Consulta: creatorFacets:"Schumann,Wolfgang"
Registros recuperados: 2
Data/hora: 11/06/2024 12:24:36

Production of recombinant proteins in Escherichia coli
Provedor de dados: 74
Autores: Schumann,Wolfgang; Ferreira,Luis Carlos S.
Attempts to obtain a recombinant protein using prokaryotic expression systems can go from a rewarding and rather fast procedure to a frustrating time-consuming experience. In most cases production of heterologous proteins in Escherichia coli K12 strains has remained an empirical exercise in which different systems are tested without a careful insight into the various factors affecting adequate expression of the encoded protein. The present review will deal with E. coli as protein factory and will cover some of the aspects related to transcriptional and translational expression signals, factors affecting protein stability and solubility and targeting of proteins to different cell compartments. Based on the knowledge accumulated over the last decade, we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Expression vectors; Secretion; Molecular chaperones.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300022

Bacillus subtilis as a tool for vaccine development: from antigen factories to delivery vectors
Provedor de dados: 42
Autores: Ferreira,Luís C.S.; Ferreira,Rita C.C.; Schumann,Wolfgang.
Bacillus subtilis and some of its close relatives have a long history of industrial and biotechnological applications. Search for antigen expression systems based on recombinant B. subtilis strains sounds attractive both by the extensive genetic knowledge and the lack of an outer membrane, which simplify the secretion and purification of heterologous proteins. More recently, genetically modified B. subtilis spores have been described as indestructible delivery vehicles for vaccine antigens. Nonetheless both production and delivery of antigens by B. subtilis strains face some inherent obstacles, as unstable gene expression and reduced immunogenicity that, otherwise, can be overcome by already available gene technology approaches. In the present review we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vaccines; Bacillus subtilis; Immunization; Protein expression.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000100009