Consulta: creatorFacets:"Senegaglia,A.C."
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Data/hora: 16/06/2024 01:50:25

The epigenetic modifiers 5-aza-2'-deoxycytidine and trichostatin A influence adipocyte differentiation in human mesenchymal stem cells
Provedor de dados: 56
Autores: Zych,J.; Stimamiglio,M.A.; Senegaglia,A.C.; Brofman,P.R.S.; Dallagiovanna,B.; Goldenberg,S.; Correa,A.
Epigenetic mechanisms such as DNA methylation and histone modification are important in stem cell differentiation. Methylation is principally associated with transcriptional repression, and histone acetylation is correlated with an active chromatin state. We determined the effects of these epigenetic mechanisms on adipocyte differentiation in mesenchymal stem cells (MSCs) derived from bone marrow (BM-MSCs) and adipose tissue (ADSCs) using the chromatin-modifying agents trichostatin A (TSA), a histone deacetylase inhibitor, and 5-aza-2′-deoxycytidine (5azadC), a demethylating agent. Subconfluent MSC cultures were treated with 5, 50, or 500 nM TSA or with 1, 10, or 100 µM 5azadC for 2 days before the initiation of adipogenesis. The differentiation was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal stem cells; Trichostatin A; 5-Aza-2′-deoxycytidine; Adipocyte differentiation; Epigenetics.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000500405