Consulta: creatorFacets:"Silva,Amanda Perse da"
Registros recuperados: 1
Data/hora: 02/06/2024 06:48:34

RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions
Provedor de dados: 55
Autores: Silva,Amanda Perse da; Lopes,Juliana Freitas; Paula,Vanessa Salete de.
The aim of this study was to evaluate the use of RNA interference to inhibit herpes simplex virus type-1 replication in vitro. For herpes simplex virus type-1 gene silencing, three different small interfering RNAs (siRNAs) targeting the herpes simplex virus type-1 UL39 gene (sequence si-UL 39-1, si-UL 39-2, and si-UL 39-3) were used, which encode the large subunit of ribonucleotide reductase, an essential enzyme for DNA synthesis. Herpes simplex virus type-1 was isolated from saliva samples and mucocutaneous lesions from infected patients. All mucocutaneous lesions' samples were positive for herpes simplex virus type-1 by real-time PCR and by virus isolation; all herpes simplex virus type-1 from saliva samples were positive by real-time PCR and 50% were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Herpes simplex virus type 1; Real-time PCR; SiRNA.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000400441