Consulta: creatorFacets:"Silva,D.G"
Registros recuperados: 2
Data/hora: 01/06/2024 06:01:42

Comparação do isolamento microbiológico e da reação em cadeia da polimerase no diagnóstico de salmonelose em bezerros infectados experimentalmente com Salmonella Typhimurium
Provedor de dados: 45
Autores: Silva,D.G; Ávila,L.G; Berg,R; Silva,D.R; Conde,S.O; Lemos,M.V.F; Fagliari,J.J.
The efficiency of microbiological culture and polymerase chain reaction (PCR) for detection of Salmonella Typhimurium is compared in fecal samples of Holstein calves experimentally infected with 10(9) CFU of Salmonella Typhimurium. Seventy-two fecal samples were analyzed by microbiological culture and PCR associated with selenite cystine (SC) and Muller-Kauffmann tethrationate (TMK) selective enrichment broths. Regardless of the selective enrichment broth, the microbiological culture was significantly better than PCR for detection of positive samples of Salmonella Typhimurium. The selective enrichment broths SC and TMK had no effect on the efficiency of the microbiological culture. The SC broth was the best option as selective enrichment associated to PCR.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovinos; Fezes; Isolamento bacteriano; Salmonella Typhimurium.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000500029

Comparação dos caldos selenito cistina, tetrationato Muller-Kauffmann e Rappaport-Vassiliadis no isolamento de Salmonella Typhimurium
Provedor de dados: 45
Autores: Ávila,L.G.; Silva,D.G; Fagliari,J.J.
Three selective enrichment broths - selenite cystine (SC), Muller-Kauffmann tetrathionate (MKT) and Rappaport-Vassiliadis (RV) - were compared, for Salmonella Typhimurium isolation from rectal swabs of a calf experimentally infected. The bacteriological procedure involved pre-enrichment in Hajna-GN broth (only for the samples inoculated in RV broth), selective enrichment (SC, MKT and RV broths), culture in modified brilliant green agar (BGA), presumptive biochemistry tests (using triple-sugar-iron agar and lysine-agar) and slide agglutination test with poli-O and poli-H Salmonella antisera. SC and MKT broths were more efficient in the isolation of Salmonella Typhimurium (12 positive samples), whereas RV broth had a lower efficiency in the microbiological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calves; Feces; Selective enrichment broth; Salmonella typhimurium; Bezerro; Fezes; Meio de enriquecimento seletivo; Salmonella Typhimurium.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000300035