Consulta: creatorFacets:"Silva,José Vieira"
Registros recuperados: 4
Data/hora: 11/06/2024 10:05:51

Expression profiles of sugarcane under drought conditions: Variation in gene regulation
Provedor de dados: Genetics and Molecular Biology
Autores: Andrade,Júlio César Farias de; Terto,Jackeline; Silva,José Vieira; Almeida,Cícero.
Abstract Drought is a major factor in decreased sugarcane productivity because of the resulting morphophysiological effects that it causes. Gene expression studies that have examined the influence of water stress in sugarcane have yielded divergent results, indicating the absence of a fixed pattern of changes in gene expression. In this work, we investigated the expression profiles of 12 genes in the leaves of a drought-tolerant genotype (RB72910) of sugarcane and compared the results with those of other studies. The genotype was subjected to 80–100% water availability (control condition) and 0–20% water availability (simulated drought). To analyze the physiological status, the SPAD index, Fv/Fm ratio, net photosynthesis (A), stomatal conductance (gs) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene expression; Plant breeding; Quantitative RT-PCR.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400465

Physiological responses of NaCl stressed cowpea plants grown in nutrient solution supplemented with CaCl2
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Silva,José Vieira; Lacerda,Claudivan Feitosa de; Costa,Paulo Henrique Alves da; Enéas Filho,Joaquim; Gomes Filho,Enéas; Prisco,José Tarquínio.
Pitiuba cowpea (Vigna unguiculata (L.) Walp.) plants were grown in nutrient solution and kept in a greenhouse up to pre-flowering stage. They were subjected to four different treatments: nutrient solution; nutrient solution containing 75 mmol.L-1 NaCl; nutrient solution containing 75 mmol.L-1 NaCl and 5 mmol.L-1 CaCl2; and nutrient solution containing 75 mmol.L-1 NaCl and 10 mmol.L-1 CaCl2. Salt stress strongly inhibited plant growth, caused a disturbance in plant-water balance, and increased the total content of inorganic solutes in the different plant parts, due mainly to accumulation of Na+ and Cl-. It also increased leaf and root soluble carbohydrates, reduced soluble amino nitrogen both in root tips and in the youngest trifoliate leaves, and reduced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium; Growth; Salt stress; Solutes accumulation; Vigna unguiculata; Water relations.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000200005

Effects of salt stress on plant growth, stomatal response and solute accumulation of different maize genotypes
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Azevedo Neto,André Dias de; Prisco,José Tarquinio; Enéas-Filho,Joaquim; Lacerda,Claudivan Feitosa de; Silva,José Vieira; Costa,Paulo Henrique Alves da; Gomes-Filho,Enéas.
Seeds from eight different maize genotypes (BR3123, BR5004, BR5011, BR5026, BR5033, CMS50, D766 and ICI8447) were sown in vermiculite, and after germination they were transplanted into nutrient solution or nutrient solution containing 100 mmol.L-1 of NaCl and placed in a greenhouse. During the experimental period plant growth (dry matter, shoot to root dry mass ratio, leaf area, relative growth rate, and net assimilation rate), leaf temperature, stomatal conductance, transpiration, predawn water potential, sodium, potassium, soluble amino acids and soluble carbohydrate contents were determined in both control and salt stressed plants of all genotypes studied. Salt stress reduced plant growth of all genotypes but the genotypes BR5033 and BR5011 were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth analysis; Osmoregulation; Salinity; Transpiration; Zea mays; Water relations.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000100005

Validation of reference genes for accurate normalization by quantitative polymerase chain reaction in sugarcane drought stress studies using two cultivars
Provedor de dados: Ciência Rural
Autores: Crystian,Dennis; Terto,Jackeline; Silva,José Vieira; Almeida,Cícero.
ABSTRACT: The aim of this study was to analyze the expression of putative reference genes in sugarcane under drought stress. The varieties RB72454 and RB72910 were cultivated and the treatments control and drought stress applied to 135-day-old plants grown under field conditions. The stress level of the plants was measured by rate of photosynthesis, transpiration, and stomatal conductance. For each biological replicate, expression analyses were conducted using quantitative polymerase chain reaction for the genes α-tubulin, β-tubulin, β-actin, cyclophilin, eukaryotic elongation factor 1, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), histone H3 and ubiquitin. Stability was evaluated using the geNorm, NormFinder, and BestKeeper software packages. Among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene expression; Drought stress; Reference gen; Saccharum.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100203