Consulta: creatorFacets:"Silva,L.B."
Registros recuperados: 6
Data/hora: 09/06/2024 03:54:52

Efeito do extrato aquoso e etanólico do angico preto sobre larvas de Rhipicephalus (Boophilus) microplus
Provedor de dados: 45
Autores: Silva Filho,M.L.; Silva,L.B.; Fernandes,R.M.; Lopes,G.S.
Rhipicephalus (Boophilus) microplus representa um grande problema na bovinocultura e o uso de acaricidas é a medida de controle profilático e terapêutico mais comum contra esses ectoparasitos. Os principais problemas relacionados com essa prática dizem respeito ao desenvolvimento de linhagens resistentes de carrapatos. Assim, objetivou-se determinar o efeito de extratos da casca de Anadenanthera macrocarpa sobre as larvas de R. (B.) microplus, obtidas de um pool de ovos, acondicionadas em tubo de polietileno. De acordo com os resultados, o extrato aquoso na concentração de 8,26mg.mL-1 causou 85% de mortalidade nas primeiras 12 horas. Quanto ao extrato etanólico, observou-se maior mortalidade nas concentrações 12,5; 6,25 e 1,56mg.mL-1, em torno de 84%,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acaricida botânico; Carrapaticida; Ectoparasitas; Angico vermelho.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000300003

Isolation and intracellular localization of insulin-like proteins from leaves of Bauhinia variegata
Provedor de dados: 56
Autores: Azevedo,C.R.; Maciel,F.M.; Silva,L.B.; Ferreira,A.T.S.; da Cunha,M.; Machado,O.L.T.; Fernandes,K.V.S.; Oliveira,A.E.A.; Xavier-Filho,J.
Evidence based on immunological cross-reactivity and anti-diabetic properties has suggested the presence of insulin-like peptides in plants. The objective of the present study was to investigate the presence of insulin-like proteins in the leaves of Bauhinia variegata ("pata-de-vaca", "mororó"), a plant widely utilized in popular medicine as an anti-diabetic agent. We show that an insulin-like protein was present in the leaves of this plant. A chloroplast protein with a molecular mass similar to that of bovine insulin was extracted from 2-mm thick 15% SDS-PAGE gels and fractionated with a 2 x 24 cm Sephadex G-50 column. The activity of this insulin-like protein (0.48 mg/mL) on serum glucose levels of four-week-old Swiss albino (CF1) diabetic mice was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bauhinia variegata; Plant insulin; Chloroplast; Medicinal plant.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100007

Lima bean (Phaseolus lunatus) seed coat phaseolin is detrimental to the cowpea weevil (Callosobruchus maculatus)
Provedor de dados: 56
Autores: Moraes,R.A.; Sales,M.P.; Pinto,M.S.P.; Silva,L.B.; Oliveira,A.E.A.; Machado,O.L.T.; Fernandes,K.V.S.; Xavier-Filho,J.
The presence of phaseolin (a vicilin-like 7S storage globulin) peptides in the seed coat of the legume Phaseolus lunatus L. (lima bean) was demonstrated by N-terminal amino acid sequencing. Utilizing an artificial seed system assay we showed that phaseolin, isolated from both cotyledon and testa tissues of P. lunatus, is detrimental to the nonhost bruchid Callosobruchus maculatus (F) (cowpea weevil) with ED50 of 1.7 and 3.5%, respectively. The level of phaseolin in the seed coat (16.7%) was found to be sufficient to deter larval development of this bruchid. The expression of a C. maculatus-detrimental protein in the testa of nonhost seeds suggests that the protein may have played a significant role in the evolutionary adaptation of bruchids to legume seeds.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lima bean; Phaseolin (vicilin); 7S storage globulin; Seed coat; Cowpea weevil; Resistance to bruchids.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200005

A protein with amino acid sequence homology to bovine insulin is present in the legume Vigna unguiculata (cowpea)
Provedor de dados: 56
Autores: Venâncio,T.M.; Oliveira,A.E.A.; Silva,L.B.; Machado,O.L.T.; Fernandes,K.V.S.; Xavier-Filho,J.
Since the discovery of bovine insulin in plants, much effort has been devoted to the characterization of these proteins and elucidation of their functions. We report here the isolation of a protein with similar molecular mass and same amino acid sequence to bovine insulin from developing fruits of cowpea (Vigna unguiculata) genotype Epace 10. Insulin was measured by ELISA using an anti-human insulin antibody and was detected both in empty pods and seed coats but not in the embryo. The highest concentrations (about 0.5 ng/µg of protein) of the protein were detected in seed coats at 16 and 18 days after pollination, and the values were 1.6 to 4.0 times higher than those found for isolated pods tested on any day. N-terminal amino acid sequencing of insulin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin; Insulin-like; Cowpea; Vigna unguiculata; Developing fruits; Sequence homology; Evolution.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900004

The leaves of green plants as well as a cyanobacterium, a red alga, and fungi contain insulin-like antigens
Provedor de dados: 56
Autores: Silva,L.B.; Santos,S.S.S.; Azevedo,C.R.; Cruz,M.A.L.; Venâncio,T.M.; Cavalcante,C.P.; Uchôa,A.F.; Astolfi Filho,S.; Oliveira,A.E.A.; Fernandes,K.V.S.; Xavier-Filho,J.
We report the detection of insulin-like antigens in a large range of species utilizing a modified ELISA plate assay and Western blotting. We tested the leaves or aerial parts of species of Rhodophyta (red alga), Bryophyta (mosses), Psilophyta (whisk ferns), Lycopodophyta (club mosses), Sphenopsida (horsetails), gymnosperms, and angiosperms, including monocots and dicots. We also studied species of fungi and a cyanobacterium, Spirulina maxima. The wide distribution of insulin-like antigens, which in some cases present the same electrophoretic mobility as bovine insulin, together with results recently published by us on the amino acid sequence of an insulin isolated from the seed coat of jack bean (Canavalia ensiformis) and from the developing fruits of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin; Insulin-like; Antigens; Evolution; Plants; Cyanobacteria; Algae; Fungi.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300004

Oral triiodothyronine for the prevention of thyroid hormone reduction in adult valvular cardiac surgery
Provedor de dados: 56
Autores: Magalhães,A.P.A.; Gus,M.; Silva,L.B.; Schaan,B.D.
Treatment of non-thyroidal illness by intravenous triiodothyronine (T3) after cardiac surgery causes a disproportional elevation of hormone levels. The administration of oral T3, which has never been studied in this context, could cause physiological hormone levels. The aim of this study was to test oral T3 for the prevention of T3 reduction during the postoperative period of valvular cardiac surgery in adults. Eighteen patients who underwent cardiac surgery for valvular disease with invasive hemodynamic monitoring were randomly assigned to 2 groups: the T group received oral T3 (N = 8), 25 µg three times/day, initiated 24 h before surgery and maintained for 48 h and the NT group (N = 10) received placebo. Serum T3, thyroxine and thyrotropin were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiac surgery; Non-thyroidal illness syndrome; Heart valve disease; Postoperative period.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700015