Consulta: creatorFacets:"Sinquin, Corinne"
Registros recuperados: 35
Data/hora: 08/06/2024 16:29:34

Characterization of biofilm extracts from two marine bacteria
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Passerini, Delphine; Fecamp, Florian; Marchand, Laetitia; Kolypczuk, Laetitia; Bonnetot, Sandrine; Sinquin, Corinne; Verrez-bagnis, Veronique; Hervio Heath, Dominique; Colliec Jouault, Sylvia; Delbarre Ladrat, Christine.
In the marine environment, biofilm formation is an important lifestyle for microorganisms. A biofilm is comprised of cells embedded in an extracellular matrix that holds them close together and keeps the biofilm attached to the colonized surface. This predominant lifestyle and its main regulation pathway, namely quorum-sensing (QS), have been shown to induce specific bioactive metabolites. In this study, we investigated the biofilm formation by two marine bacteria belonging to the Vibrio species to discover potentially innovative bioactive compounds. We proposed a protocol to isolate biofilm extracts, to analyze their biochemical composition, and to compare them to planktonic cell extracts. Cells were grown attached to a plastic surface; extracts were...
Tipo: Text
Palavras-chave: Vibrio; Biofilm; Polysaccharide; Quorum-sensing; Activity.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00592/70381/68461.pdf

Effect of low-molecular-weight fucoidan on experimental arterial thrombosis in the rabbit and rat
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Colliec-jouault, Sylvia; Millet, J; Helley, D; Sinquin, Corinne; Fischer, Am.
Tipo: Text
Palavras-chave: Arterial thrombosis; Polyaccharide; Fucoidan.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-2116.pdf

Effects of a sulfated exopolysaccharide produced by Altermonas infernus on bone biology
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Velasco, C. Ruiz; Baud'Huin, M.; Sinquin, Corinne; Maillasson, M.; Heymann, D.; Colliec-jouault, Sylvia; Padrines, M.
The growth and differentiation of bone cells is controlled by various factors which can be modulated by heparan sulphates. Here, we investigated the effects of an oversulphated exopolysaccharide (OS-EPS) on bone. We compared the effect of this compound with that of a native exopolysaccharide (EPS). Long-term administration of OS-EPS causes cancellous bone loss in mice due, in part, to an increase in the number of osteoclasts lining the trabecular bone surface. No significant difference in cancellous bone volume was found between EPS-treated mice and age-matched control mice, underlying the importance of sulphation in trabecular bone loss. However, the mechanism sustaining this osteoporosis was unclear. To clarify OS-EPS activities, we investigated the...
Tipo: Text
Palavras-chave: Bone metabolism; Bone remodeling; Exopolysaccharide; Glycosaminoglycan; Heparin.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00032/14300/15749.pdf

Assessment of biochemical methods to detect enzymatic depolymerization of polysaccharides
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rigouin, Coraline; Delbarre Ladrat, Christine; Sinquin, Corinne; Colliec-jouault, Sylvia; Dion, M.
To find biocatalyst allowing depolymerization of new polysaccharides, one needs to get a sensitive and well adapted method to a screening program. This led us to compare biochemical methods of detection of the depolymerization. Currently used methods such as reducing sugars assays, double bond monitoring or molecular weight determination were tested to follow the kinetic of depolymerization with different enzyme / polysaccharide couples. The range of concentrations of assorted enzymes allowed us to identify the most sensitive and appropriate method to detect polysaccharide degradation. Reducing sugars assays are quantitative, sensitive and almost usable with all kind of polysaccharide but some compounds may interfere with them. When polysaccharide is...
Tipo: Text
Palavras-chave: Detection; Enzyme; Depolymerization; Carbohydrate.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2008/publication-4723.pdf

Bacteria enhance the production of extracellular polymeric substances by the green dinoflagellate Lepidodinium chlorophorum
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Roux, Pauline; Siano, Raffaele; Collin, Karine; Bilien, Gwenael; Sinquin, Corinne; Marchand, Laetitia; Zykwinska, Agata; Delbarre-ladrat, Christine; Schapira, Mathilde.
High biomasses of the marine dinoflagellate Lepidodinium chlorophorum cause green seawater discolorations along Southern Brittany (NE Atlantic, France). The viscosity associated to these phenomena has been related to problems in oyster cultivation. The harmful effect of L. chlorophorum might originate from the secretion of Extracellular Polymeric Substances (EPS). To understand whether the EPS are produced by L. chlorophorum or its associated bacteria, or if they are a product of their interaction, batch cultures were performed under non-axenic and pseudo-axenic conditions for three strains. Maximum dinoflagellate cell abundances were observed in pseudo-axenic cultures. The non-sinking fraction of polymers (Soluble Extracellular Polymers, SEP), mainly...
Tipo: Text
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00682/79403/81948.pdf

Free-radical depolymerization with metallic catalysts of an exopolysaccharide produced by a bacterium isolated from a deep-sea hydrothermal vent polychaete annelid
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Petit, Anne-celine; Noiret, N; Sinquin, Corinne; Ratiskol, Jacqueline; Guezennec, Jean; Colliec-jouault, Sylvia.
Free-radical depolymerization with metallic catalysts has been investigated to depolymerize an exopolysaccharide (EPS), with a high molecular weight (&gt; 10(6) g/Mol), produced by the bacterium Alteromonas macleodii subsp. fijiensis biovar deepsane. Three metals (copper, zinc and manganese) were used either alone or mixed. These metals led to the depolymerization of the high molecular weight bacterial polysaccharide with different efficiency. This study specifies some conditions required to produce, with good reproducibility and yield, EPS derivatives of reduced molecular weight ranging from 100,000 to 20,000 g/mol. The more influential parameters are specified. This is a first study to find suitable industrial conditions in the presence of these metallic...
Tipo: Text
Palavras-chave: Bacterial exopolysaccharide; Free radical depolymerization; H202; Metal.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2146.pdf

Microcarriers Based on Glycosaminoglycan-Like Marine Exopolysaccharide for TGF-β1 Long-Term Protection
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Zykwinska, Agata; Marquis, Melanie; Godin, Mathilde; Marchand, Laetitia; Sinquin, Corinne; Garnier, Catherine; Jonchere, Camille; Chedeville, Claire; Le Visage, Catherine; Guicheux, Jerome; Colliec-jouault, Sylvia; Cuenot, Stephane.
Articular cartilage is an avascular, non-innervated connective tissue with limited ability to regenerate. Articular degenerative processes arising from trauma, inflammation or due to aging are thus irreversible and may induce the loss of the joint function. To repair cartilaginous defects, tissue engineering approaches are under intense development. Association of cells and signalling proteins, such as growth factors, with biocompatible hydrogel matrix may lead to the regeneration of the healthy tissue. One current strategy to enhance both growth factor bioactivity and bioavailability is based on the delivery of these signalling proteins in microcarriers. In this context, the aim of the present study was to develop microcarriers by encapsulating...
Tipo: Text
Palavras-chave: Exopolysaccharide; Growth factor; Microparticles; Microfluidics; Bioactivity.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00477/58882/61432.pdf

Concentration and purification of Porphyridium cruentum exopolysaccharides by membrane filtration at various cross-flow velocities
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Balti, Rafik; Le Balc'H, Romain; Brodu, Nicolas; Gilbert, Marthe; Le Gouic, Benjamin; Le Gall, Sophie; Sinquin, Corinne; Masse, Anthony.
Exopolysaccharides (EPS) from cell-free Porphyridium cruentum media were concentrated then purified (diafiltration) on a 0.14 µm ceramic membrane. The influence of cross-flow velocities on filtration performances was investigated. Mean permeate fluxes equal to 49.8, 68.9 and 81.9 L.h-1. m-2 were obtained during the concentration at 4 bar for respectively cross-flow velocities inside the membrane lumen equal to 2.5, 3.3 and 4.2 m.s-1; 49.7 L.h-1. m-2 for the diafiltration at 3.3 m.s-1. Permeate fluxes were correctly predicted from polysaccharide concentrations (10 % deviation). Volume reduction factors higher than 7.8 were reached. Rejection rates of polysaccharides and proteins varied according to the cross-flow velocities. Thus, the EPS recovery rate or...
Tipo: Text
Palavras-chave: Membrane filtration; Exopolysaccharides; Microalga; Concentration; Diafiltration.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00445/55694/57360.pdf

Antiviral Activities of Sulfated Polysaccharides Isolated from Sphaerococcus coronopifolius (Rhodophytha, Gigartinales) and Boergeseniella thuyoides (Rhodophyta, Ceramiales)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Bouhlal, Rhimou; Haslin, Camille; Chermann, Jean-claude; Colliec-jouault, Sylvia; Sinquin, Corinne; Simon, Gaelle; Cerantola, Stephane; Riadi, Hassane; Bourgougnon, Nathalie.
Water-soluble sulfated polysaccharides isolated from two red algae Sphaerococcus coronopifolius (Gigartinales, Sphaerococcaceae) and Boergeseniella thuyoides (Ceramiales, Rhodomelaceae) collected on the coast of Morocco inhibited in vitro replication of the Human Immunodeficiency Virus (HIV) at 12.5 mu g/mL. In addition, polysaccharides were capable of inhibiting the in vitro replication of Herpes simplex virus type 1 (HSV-1) on Vero cells values of EC(50) of 4.1 and 17.2 mu g/mL, respectively. The adsorption step of HSV-1 to the host cell seems to be the specific target for polysaccharide action. While for HIV-1, these results suggest a direct inhibitory effect on HIV-1 replication by controlling the appearance of the new generations of virus and...
Tipo: Text
Palavras-chave: Herpes simplex virus; Human immunodeficiency virus; Antiviral activity; Sulfated polysaccharide; S. coronopifolius; B. thuyoides; Chemical composition.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00060/17120/14631.pdf

Genome sequence of Vibrio diabolicus and identification of the exopolysaccharide HE800 biosynthesis locus
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Goudenege, David; Boursicot, Vincent; Versigny, Typhaine; Bonnetot, Sandrine; Ratiskol, Jacqueline; Sinquin, Corinne; Lapointe, Gisele; Le Roux, Frederique; Delbarre-ladrat, Christine.
Vibrio diabolicus, a marine bacterium originating from deep-sea hydrothermal vents, produces the HE800 exopolysaccharide with high value for biotechnological purposes, especially for human health. Its genome was sequenced and analyzed; phylogenetic analysis using the core genome revealed V. diabolicus is close to another deep-sea Vibrio sp. (Ex25) within the Harveyi clade and Alginolyticus group. A genetic locus homologous to the syp cluster from Vibrio fischeri was demonstrated to be involved in the HE800 production. However, few genetic particularities suggest that the regulation of syp expression may be different in V. diabolicus. The presence of several types of glycosyltransferases within the locus indicates a capacity to generate diversity in the...
Tipo: Text
Palavras-chave: Genome; Vibrio; Exopolysaccharide; Biosynthesis.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00241/35244/33892.pdf

Bioprospecting for Exopolysaccharides from Deep-Sea Hydrothermal Vent Bacteria: Relationship between Bacterial Diversity and Chemical Diversity
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Delbarre-ladrat, Christine; Salas, Marcia Leyva; Sinquin, Corinne; Zykwinska, Agata; Colliec-jouault, Sylvia.
Many bacteria biosynthesize structurally diverse exopolysaccharides (EPS) and excrete them into their surrounding environment. The EPS functional features have found many applications in industries such as cosmetics and pharmaceutics. In particular, some EPS produced by marine bacteria are composed of uronic acids, neutral sugars, and N-acetylhexosamines, and may also bear some functional sulfate groups. This suggests that they can share common structural features with glycosaminoglycans (GAG) like the two EPS (HE800 and GY785) originating from the deep sea. In an attempt to discover new EPS that may be promising candidates as GAG-mimetics, fifty-one marine bacterial strains originating from deep-sea hydrothermal vents were screened. The analysis of the...
Tipo: Text
Palavras-chave: Exopolysaccharides; GAG; Marine bacteria; Glycopolymers; Sulfate; Production.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00399/51090/51873.pdf

Sterilization of Exopolysaccharides Produced by Deep-Sea Bacteria: Impact on Their Stability and Degradation
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rederstorff, Emilie; Fatimi, Ahmed; Sinquin, Corinne; Ratiskol, Jacqueline; Merceron, Christophe; Vinatier, Claire; Weiss, Pierre; Colliec-jouault, Sylvia.
Polysaccharides are highly heat-sensitive macromolecules, so high temperature treatments are greatly destructive and cause considerable damage, such as a great decrease in both viscosity and molecular weight of the polymer. The technical feasibility of the production of exopolysaccharides by deep-sea bacteria Vibrio diabolicus and Alteromonas infernus was previously demonstrated using a bioproduct manufacturing process. The objective of this study was to determine which sterilization method, other than heat sterilization, was the most appropriate for these marine exopolysaccharides and was in accordance with bioprocess engineering requirements. Chemical sterilization using low-temperature ethylene oxide and a mixture of ionized gases (plasmas) was compared...
Tipo: Text
Palavras-chave: Marine biotechnology; Polysaccharides; Sterilization procedures; Characterization; Molecular weight distribution; Rheology.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00027/13776/10913.pdf

Pharmacological Modulation of Human Mesenchymal Stem Cell Chondrogenesis by a Chemically Oversulfated Polysaccharide of Marine Origin: Potential Application to Cartilage Regenerative Medicine
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Merceron, Christophe; Portron, Sophie; Vignes-colombeix, Caroline; Rederstorff, Emilie; Masson, Martial; Lesoeur, Julie; Sourice, Sophie; Sinquin, Corinne; Colliec-jouault, Sylvia; Weiss, Pierre; Vinatier, Claire; Guicheux, Jerome.
Mesenchymal stem cells (MSCs) are considered as an attractive source of cells for cartilage engineering due to their availability and capacity for expansion and multipotency. Differentiation of MSC into chondrocytes is crucial to successful cartilage regeneration and can be induced by various biological agents, including polysaccharides that participate in many biological processes through interactions with growth factors. Here, we hypothesize that growth factor-induced differentiation of MSC can be increased by chemically oversulfated marine polysaccharides. To test our hypothesis, human adipose tissue-derived MSCs (hATSCs) were cultured in pellets with transforming growth factor (TGF)-beta 1-supplemented chondrogenic medium containing either the...
Tipo: Text
Palavras-chave: Cartilage; Adipose-derived mesenchymal stem cells; Polysaccharides; Transforming growth factor-beta.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00071/18227/17457.pdf

Enzymatic depolymerization of the GY785 exopolysaccharide produced by the deep-sea hydrothermal bacterium Alteromonas infernus : structural study and enzyme activity assessment
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Zykwinska, Agata; Berre, Ludivine Tripon-le; Sinquin, Corinne; Ropartz, David; Rogniaux, Helene; Colliec-jouault, Sylvia; Delbarre-ladrat, Christine.
Polysaccharides have attracted much attention due to their interesting physico-chemical and also biological properties that are explored in food, cosmetic and pharmaceutical industries. GY785 exopolysaccharide (EPS) presenting an unusual structure is secreted by the deep-sea hydrothermal bacterium, Alteromonas infernus. Low-molecular weight (LMW) derivatives obtained by chemical depolymerization of the native high molecular weight (HMW) EPS were previously shown to exhibit biological properties similar to glycosaminoglycans (GAG). In the present study, in order to generate well defined derivatives with a better control of the depolymerization, an enzymatic approach was applied for the first time. Various commercially available enzymes were firstly screened...
Tipo: Text
Palavras-chave: Exopolysaccharide; Enzyme; Depolymerization; Oligosaccharide; Mass spectrometry.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00423/53458/54352.pdf

Antiproliferative Properties of Scandium Exopolysaccharide Complexes on Several Cancer Cell Lines
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Muñoz-garcia, Javier; Mazza, Mattia; Alliot, Cyrille; Sinquin, Corinne; Colliec-jouault, Sylvia; Heymann, Dominique; Huclier-markai, Sandrine.
Antimetastatic properties on both murine and human osteosarcoma cell lines (POS-1 and KHOS) have been evidenced using exopolysaccharide (EPS) derivatives, produced by Alteromonas infernus bacterium. These derivatives had no significant effect on the cell cycle neither a pro-apoptotic effect on osteosarcoma cells. Based on this observation, these EPSs could be employed as new drug delivery systems for therapeutic uses. A theranostic approach, i.e., combination of a predictive biomarker with a therapeutic agent, has been developed notably by combining with true pair of theranostic radionuclides, such as scandium 47Sc/44Sc. However, it is crucial to ensure that, once complexation is done, the biological properties of the vector remain intact, allowing the...
Tipo: Text
Palavras-chave: Exopolysaccharides; Scandium; Theranostic; Cancer cell lines; Proliferation.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00686/79826/82630.pdf

An in vitro study of two GAG-like marine polysaccharides incorporated into injectable hydrogels for bone and cartilage tissue engineering
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rederstorff, Emilie; Weiss, Pierre; Sourice, S.; Pilet, P.; Xie, F.; Sinquin, Corinne; Colliec-jouault, Sylvia; Guicheux, Jerome; Laib, S.
Natural polysaccharides are attractive compounds with which to build scaffolds for bone and cartilage tissue engineering. Here we tested two non-standard ones, HE800 and GY785, for the two-dimensional (2-D) and three-dimensional (3-D) culture of osteoblasts (MC3T3-E1) and chondrocytes (C28/I2). These two glycosaminoglycan-like marine exopolysaccharides were incorporated into an injectable silylated hydroxypropylmethylcellulose-based hydrogel (Si-HPMC) that has already shown its suitability for bone and cartilage tissue engineering. Results showed that, similarly to hyaluronic acid (HA) (the control), HE800 and GY785 significantly improved the mechanical properties of the Si-HPMC hydrogel and induced the attachment of MC3T3-E1 and C28/I2 cells when these...
Tipo: Text
Palavras-chave: Glycosaminoglycan; Hydrogel; Polysaccharide; Bone and cartilage tissue engineering; In vitro test.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00030/14090/15747.pdf

Screening of enzymatic activities for the depolymerisation of the marine bacterial exopolysaccharide HE800
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rigouin, Coraline; Delbarre-ladrat, Christine; Ratiskol, Jacqueline; Sinquin, Corinne; Colliec-jouault, Sylvia; Dion, Michel.
Tipo: Text
Palavras-chave: Polysaccharide; Enzymatic depolymerisation; Low-molecular-weight derivatives; Enterococcus faecalis; Endo-N-acetyl-galactosaminidase.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00098/20907/18741.pdf

Emulsifying Activity and Stability of a Non-Toxic Bioemulsifier Synthesized by Microbacterium sp MC3B-10
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Camacho-chab, Juan Carlos; Guezennec, Jean; Chan-bacab, Manuel Jesus; Rios-leal, Elvira; Sinquin, Corinne; Muniz-salazar, Raquel; De La Rosa-garcia, Susana Del C.; Reyes-estebanez, Manuela; Ortega-morales, Benjamin Otto.
A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its...
Tipo: Text
Palavras-chave: Bioemulsifier; Emulsifying stability; Non-toxic; Microbacterium sp; Biotechnology.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00171/28260/26530.pdf

Deep-sea Hydrothermal Vent Bacteria as a Source of Glycosaminoglycan-Mimetic Exopolysaccharides
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Zykwinska, Agata; Marchand, Laetitia; Bonnetot, Sandrine; Sinquin, Corinne; Colliec-jouault, Sylvia; Delbarre-ladrat, Christine.
Bacteria have developed a unique strategy to survive in extreme environmental conditions through the synthesis of an extracellular polymeric matrix conferring upon the cells a protective microenvironment. The main structural component of this complex network constitutes high-molecular weight hydrophilic macromolecules, namely exopolysaccharides (EPS). EPS composition with the presence of particular chemical features may closely be related to the specific conditions in which bacteria evolve. Deep-sea hydrothermal vent bacteria have already been shown to produce EPS rich in hexosamines and uronic acids, frequently bearing some sulfate groups. Such a particular composition ensures interesting functional properties, including biological activities mimicking...
Tipo: Text
Palavras-chave: Exopolysaccharide; Glycosaminoglycan; Uronic acid; Hexosamine; Phylogenetic analysis.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00506/61756/65728.pdf

Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Norris, Karl; Mishukova, Oksana; Zykwinska, Agata; Colliec-jouault, Sylvia; Sinquin, Corinne; Koptioug, Andrei; Cuenot, Stéphane; Kerns, Jemma; Surmeneva, Maria; Surmenev, Roman; Douglas, Timothy.
Polysaccharides of marine origin are gaining interest as biomaterial components. Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior. The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored. In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization. Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of...
Tipo: Text
Palavras-chave: Marine exopolysaccharide; Collagen; Surface modification; Ti6Al4V.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00477/58881/61433.pdf

Characterization of New Oligosaccharides Obtained by An Enzymatic Cleavage of the Exopolysaccharide Produced by the Deep-Sea Bacterium Alteromonas infernus Using its Cell Extract
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Akoumany, Katy; Zykwinska, Agata; Sinquin, Corinne; Marchand, Laetitia; Fanuel, Mathieu; Ropartz, David; Rogniaux, Hélène; Pipelier, Muriel; Delbarre Ladrat, Christine; Colliec Jouault, Sylvia.
Bacteria from deep-sea hydrothermal vents constitute an attractive source of bioactive molecules. In particular, exopolysaccharides (EPS) produced by these bacteria become a renewable source of both biocompatible and biodegradable molecules. The low molecular weight (LMW) derivatives of the GY785 EPS produced by the deep-sea hydrothermal vent strain Alteromonas infernus have previously displayed some biological properties, similar to those of glycosaminoglycans (GAG), explored in cancer and tissue engineering. These GAG-mimetic derivatives are obtained through a free radical depolymerization process, which could, however, affect their structural integrity. In a previous study, we have shown that A. infernus produces depolymerizing enzymes active on its own...
Tipo: Text
Palavras-chave: Deep-sea bacterium; Alteromonas infernus; Wild-type strain; Exopolysaccharides; Glycosaminoglycan-mimetic; Enzymatic depolymerization; Structural analysis; Mass spectrometry.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00515/62644/67021.pdf

Exopolysaccharides produced by marine bacteria and their applications as glycosaminoglycan-like molecules
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Delbarre-ladrat, Christine; Sinquin, Corinne; Lebellenger, Lou; Zykwinska, Agata; Colliec-jouault, Sylvia.
Although polysaccharides are ubiquitous and the most abundant renewable bio-components, their studies, covered by the glycochemistry and glycobiology fields, remain a challenge due to their high molecular diversity and complexity. Polysaccharides are industrially used in food products; human therapeutics fall into a more recent research field and pharmaceutical industry is looking for more and more molecules with enhanced activities. Glycosaminoglycans (GAGs) found in animal tissues play a critical role in cellular physiological and pathological processes as they bind many cellular components. Therefore, they present a great potential for the design and preparation of therapeutic drugs. On the other hand, microorganisms producing exopolysaccharides (EPS)...
Tipo: Text
Palavras-chave: Marine-derivedpolysaccharide; Biodiversity; Biologicalactivity; Structure; Modification; GAG-mimetic; Biosynthesis.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00241/35250/33764.pdf

Marine Polysaccharides: A Source of Bioactive Molecules for Cell Therapy and Tissue Engineering
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Senni, Karim; Pereira, Jessica; Gueniche, Farida; Delbarre-ladrat, Christine; Sinquin, Corinne; Ratiskol, Jacqueline; Godeau, Gaston; Fischer, Anne-marie; Helley, Dominique; Colliec-jouault, Sylvia.
The therapeutic potential of natural bioactive compounds such as polysaccharides, especially glycosaminoglycans, is now well documented, and this activity combined with natural biodiversity will allow the development of a new generation of therapeutics. Advances in our understanding of the biosynthesis, structure and function of complex glycans from mammalian origin have shown the crucial role of this class of molecules to modulate disease processes and the importance of a deeper knowledge of structure-activity relationships. Marine environment offers a tremendous biodiversity and original polysaccharides have been discovered presenting a great chemical diversity that is largely species specific. The study of the biological properties of the...
Tipo: Text
Palavras-chave: Marine bacteria; Marine algae; Exopolysaccharides; Sulfated polysaccharides; Structure; Chemical modification; Biological activity; Blue biotechnology; Cell therapy; Tissue engineering.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00048/15894/13316.pdf

Marine Exopolysaccharide Complexed with Scandium Aimed as Theranostic Agents
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Mazza, Mattia; Alliot, Cyrille; Sinquin, Corinne; Colliec-jouault, Sylvia; Reiller, Pascal E.; Huclier-markai, Sandrine.
Background: Exopolysaccharide (EPS) derivatives, produced by Alteromonas infernus bacterium, showed anti-metastatic properties. They may represent a new class of ligands to be combined with theranostic radionuclides, such as 47Sc/44Sc. The goal of this work was to investigate the feasibility of such coupling. (2) Methods: EPSs, as well as heparin used as a drug reference, were characterized in terms of molar mass and dispersity using Asymmetrical Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering (AF4-MALS). The intrinsic viscosity of EPSs at different ionic strengths were measured in order to establish the conformation. To determine the stability constants of Sc with EPS and heparin, a Free-ion selective radiotracer extraction (FISRE)...
Tipo: Text
Palavras-chave: Exopolysaccharides; Scandium; Theranostic; Characterization; Complexation.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00681/79332/81844.pdf

Assembly of HE800 exopolysaccharide produced by a deep-sea hydrothermal bacterium into microgels for protein delivery applications
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Zykwinska, Agata; Marquis, Melanie; Sinquin, Corinne; Cuenot, Stephane; Colliec-jouault, Sylvia.
Assembly of biopolymers into microgels is an elegant strategy for bioencapsulation with various potential biomedical applications. Such biocompatible and biodegradable microassemblies are developed not only to protect the encapsulated molecule but also to ensure its sustained local delivery. The present study describes the fabrication of microassemblies from a marine HE800 exopolysaccharide (EPS), which displays a glycosaminoglycan (GAG)-like structure and biological properties. HE800 EPS was assembled, through physical cross-linking with divalent ions, into microgel particles and microfibers using microfluidics. The microparticle morphology was highly affected by the polysaccharide concentration and its molecular weight. A model protein, namely Bovine...
Tipo: Text
Palavras-chave: Marine exopolysaccharide; Microgel particles; Microfibers; Microfluidic; Microencapsulation; Protein release.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00311/42175/41475.pdf

Emulsifying Activity and Stability of a Non-Toxic Bioemulsifier Synthesized by Microbacterium sp MC3B-10
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Camacho-chab, Juan Carlos; Guezennec, Jean; Chan-bacab, Manuel Jesus; Rios-leal, Elvira; Sinquin, Corinne; Muniz-salazar, Raquel; De La Rosa-garcia, Susana Del C.; Reyes-estebanez, Manuela; Ortega-morales, Benjamin Otto.
A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its...
Tipo: Text
Palavras-chave: Bioemulsifier; Emulsifying stability; Non-toxic; Microbacterium sp; Biotechnology.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00372/48356/48545.pdf

Exopolysaccharides produced by bacteria isolated from deep-sea hydrothermal vents: new agents with therapeutic potential
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Colliec-jouault, Sylvia; Zanchetta, P; Helley, D; Ratiskol, Jacqueline; Sinquin, Corinne; Fischer, A. M.; Guezennec, Jean.
Bacterial polysaccharides offer fascinating potential applications for the pharmaceutical industry. Although many known marine bacteria produce exopolysaccharides (EPS), continuation in looking for new polysaccharide-producing micro-organisms is promising. Marine bacteria, isolated from deep-sea hydrothermal vents, have demonstrated their ability to produce in aerobic conditions, unusual EPS. With the aim of discovering biological activities, EPS presenting different structural features were studied. An EPS secreted by Vibrio, diabolicus was evaluated on the restoration of bone integrity in experimental model and was demonstrated to be a strong bone-healing material. Another EPS produced by Alteromonas infernus was modified in order to obtain new...
Tipo: Text
Palavras-chave: Hydrothermal vents; Exopolysaccharides; Bone healing; Biomimetic; Anticoagulant activity; Sources hydrothermales; Exopolysaccharide; Cicatrisation osseuse; Biomimétique; Activité anticoagulante.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-2117.pdf

Anti-Metastatic Properties of a Marine Bacterial Exopolysaccharide-Based Derivative Designed to Mimic Glycosaminoglycans
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Heymann, Dominique; Ruiz-velasco, Carmen; Chesneau, Julie; Ratiskol, Jacqueline; Sinquin, Corinne; Colliec-jouault, Sylvia.
Osteosarcoma is the most frequent malignant primary bone tumor characterized by a high potency to form lung metastases. In this study, the effect of three oversulfated low molecular weight marine bacterial exopolysaccharides (OS-EPS) with different molecular weights (4, 8 and 15 kDa) were first evaluated in vitro on human and murine osteosarcoma cell lines. Different biological activities were studied: cell proliferation, cell adhesion and migration, matrix metalloproteinase expression. This in vitro study showed that only the OS-EPS 15 kDa derivative could inhibit the invasiveness of osteosarcoma cells with an inhibition rate close to 90%. Moreover, this derivative was potent to inhibit both migration and invasiveness of osteosarcoma cell lines; had no...
Tipo: Text
Palavras-chave: Exopolysaccharides; Glycosaminoglycan; Heparin-like; Derivatives; Sulfation; Bone metabolism; Bone remodeling; Lung mestatases; Osteosarcoma.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00333/44442/44112.pdf

Effect of an oversulfated exopolysaccharide on angiogenesis induced by fibroblast growth factor-2 or vascular endothelial growth factor in vitro
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Matou, Sabine; Colliec-jouault, Sylvia; Galy Fauroux, Isabelle; Ratiskol, Jacqueline; Sinquin, Corinne; Guezennec, Jean; Fischer, Anne-marie; Helley, Dominique.
The aim of this study was to determine the angiogenic properties of an oversulfated exopolysaccharide (OS-EPS) derived from a polysaccharide secreted by the mesophilic bacterium Alteromonas infernus. We compared the effect of this OS-EPS with that of a non-oversulfated exopolysaccharide (EPS) on human umbilical vein endothelial cell (HUVEC) proliferation, migration and differentiation induced by basic fibroblast growth factor (FGF-2) or vascular endothelial growth factor (VEGF). OS-EPS enhanced HUVEC proliferation by 58% when used alone, and by respectively 30% and 70% in the presence of FGF-2 and VEGF. OS-EPS also increased the density of tubular structures on Matrigel in the presence of FGF-2 or VEGF. Vascular tube fort-nation was related to alpha(6)...
Tipo: Text
Palavras-chave: Matrigel; Collagen gel; Alpha 6 integrin subunit; Angiogenic growth factor; Polysaccharide; Angiogenesis.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-2118.pdf

Characterization, chemical modifications and in vitro anticoagulant properties of an exopolysaccharide produced by Alteromonas infernus
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Colliec-jouault, Sylvia; Chevolot, Lionel; Helley, Dominique; Ratiskol, Jacqueline; Bros, Andrée; Sinquin, Corinne; Roger, Olivier; Fischer, Anne-marie.
A new low-molecular-weight 'heparin-like' component was obtained from an exopolysaccharide produced by a mesophilic strain found in deep-sea hydrothermal vents. Data concerning the structure of the native high-molecular-weight exopolysaccharide (10(6) g/mol, 10% sulfate content) are reported for the first time. Two depolymerization processes were used to obtain low-molecular-weight (24-35 x 10(3) g/mol) oversulfated fractions (sulfate content 20 or 40%), Nuclear magnetic resonance studies indicated that after sulfation (40%), the low-molecular-weight fraction obtained by free radical depolymerization was less sulfated in the 6-O-position than the fraction depolymerized by acid hydrolysis. The free radical depolymerized product also had sulfated residues in...
Tipo: Text
Palavras-chave: Sulfation; Sulfated polysaccharide; Serpin; Heparin like; Depolymerization; Anticoagulant activity; Affinity co electrophoresis.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-2111.pdf

Polysaccharide labelling: impact on structural and biological properties
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Roger, Olivier; Colliec-jouault, Sylvia; Ratiskol, Jacqueline; Sinquin, Corinne; Guezennec, Jean; Fischer, A; Chevolot, Lionel.
Three sulphated polysaccharides labelled at the reducing end with three different chemical treatments (2-aminopyridine (2-AP), 8-amino-1,3,6-trisulphonic acid (ANTS) and 6-(biotinyl)-aminocaproyl-hydrazide (BACH)) were compared. Molecular weight, chemical composition, nuclear magnetic resonance features and anticoagulant activity expressed as activated partial thromboplastin time were studied to determine whether structural and biological properties were retained or not after labelling. Partial depolymerisation and reduced biological activity were observed when reductive amination was performed with 2-AP. The initial properties were better retained with ANTS, and BACH did not modify the anticoagulant and structural properties. (C) 2002 Elsevier Science...
Tipo: Text
Palavras-chave: Anticoagulant activity; Reductive amination; Fluorescent tagging; Fucan; GAG; Sulphated polysaccharide.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-2113.pdf

Rapport sur l'estimation de l'eau ajoutée par trempage aux noix de coquilles Saint-Jacques
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Han Ching, Lucay; Hadjadj, Annie; Sinquin, Corinne.
Les mollusques congelés représentent une part de plus en plus importante de nos importations de produits de la mer. Destinées à la consommation directe ou à la transformation, les noix de coquilles Saint Jacques congelées sont importées du Japon, du Royaume Uni ou d'Australie, en général glazurées individuellement, et souvent après trempage préalable destiné à les faire absorber une certaine quantité d'eau. C'est ainsi que l'eau totale ajoutée à la noix (glaçage + trempage) peut représenter 50 % du poids du produit avant traitement.  A la demande conjointe de l'administration et de la profession, une méthode de détermination de l'eau de glaçage ajoutée a été mise au point au sein d'un groupe de travail animé par la FICUR. Dans ce même contexte, le besoin...
Tipo: Text
Ano: 1987
URL: http://archimer.ifremer.fr/doc/00099/20988/18614.pdf

Purification of the exopolysaccharide produced by Alteromonas infernus: identification of endotoxins and effective process to remove them
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Grivaud-le Du, Alicia; Zykwinska, Agata; Sinquin, Corinne; Ratiskol, Jacqueline; Weiss, Pierre; Vinatier, Claire; Guicheux, Jerome; Delbarre-ladrat, Christine; Colliec-jouault, Sylvia.
Alteromonas infernus bacterium isolated from deep-sea hydrothermal vents can produce by fermentation a high molecular weight exopolysaccharide (EPS) called GY785. This EPS described as a new source of glycosaminoglycan-like molecule presents a great potential for pharmaceutical and biotechnological applications. However, this unusual EPS is secreted by a Gram-negative bacterium and can be therefore contaminated by endotoxins, in particular the lipopolysaccharides (LPS). Biochemical and chemical analyses of the LPS extracted from A. infernus membranes have shown the lack of the typical LPS architecture since 3-deoxy-d-manno-oct-2-ulopyranosonic acid (Kdo), glucosamine (GlcN), and phosphorylated monosaccharides were not present. Unlike for other...
Tipo: Text
Palavras-chave: Marine Alteromonas infernus; Bacterial exopolysaccharide; Endotoxin; Characterization; Purification; Fermentation; Process.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00397/50818/51601.pdf

Unusual Glycosaminoglycans from a Deep Sea Hydrothermal Bacterium Improve Fibrillar Collagen Structuring and Fibroblast Activities in Engineered Connective Tissues
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Senni, Karim; Gueniche, Farida; Changotade, Sylvie; Septier, Dominique; Sinquin, Corinne; Ratiskol, Jacqueline; Lutomski, Didier; Godeau, Gaston; Guezennec, Jean; Colliec-jouault, Sylvia.
Biopolymers produced by marine organisms can offer useful tools for regenerative medicine. Particularly, HE800 exopolysaccharide (HE800 EPS) secreted by a deep-sea hydrothermal bacterium displays an interesting glycosaminoglycan-like feature resembling hyaluronan. Previous studies demonstrated its effectiveness to enhance in vivo bone regeneration and to support osteoblastic cell metabolism in culture. Thus, in order to assess the usefulness of this high-molecular weight polymer in tissue engineering and tissue repair, in vitro reconstructed connective tissues containing HE800 EPS were performed. We showed that this polysaccharide promotes both collagen structuring and extracellular matrix settle by dermal fibroblasts. Furthermore, from the native HE800...
Tipo: Text
Palavras-chave: Marine hydrothermal bacteria; Glycosaminoglycan-mimetic; Collagen; Matrix metalloproteinases; Dermal fibroblast; Polysaccharides; Tissue engineering.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00138/24973/23072.pdf

Structural data on a bacterial exopolysaccharide produced by a deep-sea Alteromonas macleodii strain
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Le Costaouec, Tinaig; Cerantola, Stephane; Ropartz, D.; Ratiskol, Jacqueline; Sinquin, Corinne; Colliec-jouault, Sylvia; Boisset, Claire.
Some marine bacteria collected around deep-sea hydrothermal vents are able to produce, in laboratory conditions, complex and innovative exopolysaccharides. In a previous study, the mesophilic strain Alteromonas macleodii subsp. fijiensis biovar deepsane was collected on the East Pacific Rise at 2600m depth. It was isolated from a polychaete annelid Alvinella pompejana and is able to synthesise and excrete the exopolysaccharide deepsane. Biological activities have been screened and some protective properties have been established. Deepsane is commercially available in cosmetics under the name of Abyssine (R) for soothing and reducing irritation of sensitive skin against chemical, mechanical and UVB aggression. This study presents structural data for this...
Tipo: Text
Palavras-chave: Bacterial exopolysaccharide; Alteromonas macleodii; Structure determination; NMR spectroscopy; Mass spectrometry.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00095/20620/18536.pdf