Consulta: creatorFacets:"Sommerer, N"
Registros recuperados: 1
Data/hora: 11/06/2024 06:53:17

The effect of environmental salinity on the proteome of the sea bass (Dicentrarchus labrax L.)
Provedor de dados: 5
Autores: Ky, Chin Long; De Lorgeril, Julien; Hirtz, C; Sommerer, N; Rossignol, M; Bonhomme, F.
The European sea bass, Dicentrarchus labrax L., tolerates a range of salinities from freshwater to hyper-saline. To study differences in protein expression, fish were reared in both freshwater and seawater. After 3-month acclimation, gill and intestine epithelia were collected and the soluble protein extracted. In all, 362 spots were differentially expressed in the gills and intestines of fishes reared in seawater compared to those from freshwater. Fifty differential protein spots were excised from a colloidal Coomassie-stained gel. Nine separate protein spots were identified unambiguously by mass spectrometry and database searching. Among the six proteins over-expressed in gill cells in seawater, five were cytoskeleton proteins and one was the aromatase...
Tipo: Text
Palavras-chave: Two dimensional gel electrophoresis; Sea bass; Salinity; Real time PCR; MALDI TOF mass spectrometry.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3952.pdf