Consulta: creatorFacets:"Soriano,Leonardo"
Registros recuperados: 2
Data/hora: 17/06/2024 01:37:24

In vitro organogenesis and genetic transformation of mandarin cultivars
Provedor de dados: 94
Autores: Soriano,Leonardo; Tavano,Eveline Carla da Rocha; Correa,Marcelo Favaretto; Harakava,Ricardo; Mendes,Beatriz Madalena Januzzi; Mourão Filho,Francisco de Assis Alves.
Abstract  The in vitro organogenesis of Fremont (Citrus clementina x ), Citrus reticulataThomas (Citrus reticulata), and Nules (Citrus clementina) mandarins was evaluated aiming to optimize a regeneration protocol that could be applied in genetic transformation. The use of epicotyl-derived explants resulted in higher explant responsiveness and number of shoots developed per explant when compared with the use of internodal-derived explants. The highest efficiency in shoot regeneration was observed in the presence of 1 mg L-1 of BAP, regardless of the explant type and cultivar. The in vitro organogenesis protocol produced transgenic plants from three mandarin cultivars expressing attA gene under the control of phloem-specific promoters.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial peptide; Cultivar improvement; Cytokinin; Huanglongbing; Transgenic plants.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000200802

In vitro organogenesis of rangpur lime
Provedor de dados: 94
Autores: Soriano,Leonardo; Tavano,Eveline Carla da Rocha; Behling,Maurel; Mourão Filho,Francisco de Assis Alves; Mendes,Beatriz Madalena Januzzi.
Rangpur lime (Citrus limonia Osbeck) in vitro organogenesis was studied based on explant type and cytokinin culture media supplementation. Four explants types collected from epicotyl or hypocotyl regions of in vitro germinated seedlings were evaluated. The epicotyl-derived explants consisted of epicotyl segments and the hypocotyl-derived explants consisted of the entire hypocotyl segment, the hypocotyl segment attached to a cotyledon fragment, and the hypocotyl segment divided longitudinally. The explants were cultured on EME culture medium supplemented with benzylaminopurine (0, 0.5, 1.0, or 1.5 mg L-1). The evaluation was performed after 6 weeks. Best results considering both the explant responsiveness and number of shoots developed per explants were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adventitious shoot; Citrus limonia; Tissue culture.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452012000200006