Consulta: creatorFacets:"Souza,Antonio da Silva"
Registros recuperados: 3
Data/hora: 13/06/2024 01:32:47

Regeneração de plantas de laranja 'Pêra' via organogênese in vitro
Provedor de dados: 86
Autores: Silva,Rosely Pereira da; Costa,Maria Angélica Pereira de Carvalho; Souza,Antonio da Silva; Almeida,Weliton Antonio Bastos de.
O objetivo deste trabalho foi estabelecer um protocolo eficiente de regeneração de plantas in vitro, via organogênese em explante juvenil de laranja 'Pêra' (Citrus sinensis L. Osbeck), para atender futuros trabalhos de transformação genética. Segmentos de epicótilo utilizados como explantes foram introduzidos em meio de cultura MT. A fim de maximizar a regeneração de plantas in vitro, foram realizados experimentos para avaliar as concentrações de BAP no meio de cultivo (0, 1, 2, 3 ou 4 mg L-1), tamanho (0,25, 0,5 ou 1 cm), polaridade (basal, medial e apical), posição (horizontal ou vertical), condições de luminosidade (fotoperíodo de 16 horas e escuro por 30 dias) e seccionamento nas extremidades dos explantes, bem como melhores condições para garantir...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus sinensis; Cultura de tecidos; Explante juvenil; Benzilaminopurina; Microenxertia.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005001200001

Morphogenetic response of cotyledon and leaf explants of melon (Cucumis melo L.) cv. Amarillo Oro
Provedor de dados: 52
Autores: Souza,Fernanda Vidigal Duarte; Garcia-Sogo,Begoña; Souza,Antonio da Silva; San-Juán,Amparo Pérez; Moreno,Vicente.
Callus cultures from cotyledon and leaf explants of a Spanish cultivar of melon (Amarillo Oro) were tested for growth and morphogenic capacity on several culture media with different concentrations of IAA (indole-3-acetic acid) in combination with 1.0 mg.L-1 BA (6-benzylaminopurine) or 6.0 mg.L-1 KIN (kinetin). The best results were achieved with cotyledon explants. The leaf explants presented low bud formation capacity. Variability of organogenic response on cotyledons of different age (7, 5, 3 and 1-day-old) was evaluated. The age of explant had a significant influence on bud induction. Cotyledon explants from 7-day-old seedlings showed higher organogenic index and development of shoots when cultured onto MS medium supplemented with 1.5 mg.L-1 of IAA and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Organogenesis; Plant regeneration; Leaf explant; Cotyledon explant; Cucurbitaceae.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100003

Micropropagation and in vitro conservation of Neoglaziovia variegata (Arr. Cam.) mez, a fiber producing bromeliad from Brazil
Provedor de dados: 52
Autores: Silveira,Daniela Garcia; Souza,Fernanda Vidigal Duarte; Pelacani,Claudinéia Regina; Souza,Antonio da Silva; Ledo,Carlos Alberto da Silva; Santana,José Raniere Ferreira de.
Neoglaziovia variegata (Arr. Cam.) Mez is a Bromeliaceae native to the Caatinga, used for fiber extraction in the Northeast Region of Brazil. The antropic activity has place this species among the threatened ones. The objective of the work was to establish an in vitro propagation and conservation of caroá. Seeds were cultivated in MS medium in the presence or absence of light. In vitro germinated seedlings were multiplied in MS medium supplemented with the combinations 0.05 and 0.50 µM NAA and 2.2 and 4.4 µM BAP and KIN. The best percentages of germination were obtained with the seeds incubated in the presence of light. The highest multiplication ratio was obtained for the NAA (0,5 µM) + BAP (4,4 µM) treatment and the number of roots, with NAA (0.5 µM) +...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acclimatization; Bromeliaceae; Fiber extraction; In vitro preservation; Seedling production.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400016