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Conformational analysis of Pneumococcal Surface Antigen A (PsaA) upon zinc binding by fluorescence spectroscopy
Provedor de dados: 42
Autores: Bom,Ana Paula D. Ano; Corrêa,Izabella B.S.; Argondizzo,Ana Paula C.; Medeiros,Marco Alberto; Santos,Roger B. dos; Souza,Theo Luiz F. de; Silva Junior,José G. da.
ABSTRACT PsaA (pneumococcal surface antigen A) is a S. pneumoniae virulence factor that belongs to the metal transport system. The Manganese PsaA binding has been associated with oxidative stress resistance becoming a pivotal element in the bacteria virulence. It has been shown that Zinc inhibits the Manganese acquisition and promotes bacteria toxicity. We have performed a PsaA conformational analysis both in the presence (Zn-rPsaA) and in the absence of Zinc (free-rPsaA). We performed experiments in the presence of different Zinc concentrations to determine the metal minimum concentration which induced a conformational change. The protein in free and Zn-binding condition was also studied in pH ranging 2.6-8.0 and in temperature ranging 25oC-85oC. pH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conformational analysis; RPsaA; Zinc; Stability; Streptococcus Pneumoniae.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502299