Consulta: creatorFacets:"Suquet, Marc"
Registros recuperados: 77
Data/hora: 04/06/2024 02:41:09

pH controls spermatozoa motility in the Pacific oyster (     Crassostrea gigas     )
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boulais, Myrina; Suquet, Marc; Arsenault-pernet, Marie Eve Julie; Malo, Florent; Queau, Isabelle; Pignet, Patricia; Ratiskol, Dominique; Le Grand, Jacqueline; Huber, Matthias; Cosson, Jacky.
Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the Pacific oyster. The acidic pH of the gonad (5.82 ± 0.22) maintained sperm in the quiescent stage and initiation of flagellar movement was triggered by a sudden increase of spermatozoa external pH (pHe) when released in seawater (SW). At pH 6.4, percentage of motile spermatozoa was three times higher when they were activated in SW containing 30 mM NH4Cl, which alkalinizes internal pH (pHi) of spermatozoa, compared...
Tipo: Text
Palavras-chave: Seminal fluid; Spermatozoa; Motility; PH; Salinity; Ions.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00428/53976/55137.pdf

Growth and reproductive performances of juveniles and sexually mature pollack Pollachius pollachius in different conditions
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Omnes, Marie-helene; Severe, Armelle; Barone, Herve; Suquet, Marc; Buchet, Vincent; Le Roux, Annick; Gaignon, Jean-louis; Fostier, Alexis; Fauvel, Christian.
The pollack Pollachius pollachius is a commercially important fish, considered as a candidate for aquaculture in France. Rearing attempts are promising (Suquet et al., 1996), and more information is required about its reproduction, to contribute to a better management of breeders for this species. This study was designed to examine the first maturity in pollack and the effects of conditions such as origin and delayed reproduction season, on gonad maturation, reproductive and growth performances of adults.
Tipo: Text
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00002/11324/7861.pdf

Influence of dry diets on reproductive performance and egg lipid composition during the first spawning season of captive pollack
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Omnes, Marie-helene; Recek, Sophie; Barone, Herve; Le Delliou, Herve; Schmitz, Audrey; Mutelet, Alexis; Suquet, Marc; Robin, Jean H.
Four‐year‐old pollack Pollachius pollachius L., previously fed on dry pellets since their juvenile stage, were divided into three experimental groups two months prior their first spawning season. They were fed a commercial broodstock pellet enriched with 6% added oils, either: (1) capelin oil (control), (2) capelin oil plus arachidonic acid, or (3) DHA rich tuna oil. Spawning performance was determined in each group and egg lipids were analysed. During the vitellogenic period, the estradiol levels in plasma increased with oocyte enlargement, indicating that captivity and pelleted feed did not affect reproductive capacity. Females from each group spawned spontaneously between February and May. Egg production per kg of female was highest in the control...
Tipo: Text
Ano: 2004
URL: http://archimer.ifremer.fr/doc/00002/11322/7859.pdf

Sperm features of captive Atlantic bluefin tuna (Thunnus thynnus)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Cosson, J.; De La Gandara, F.; Mylonas, C. C.; Papadaki, M.; Lallemant, S.; Fauvel, Christian.
P&gt;The present study aimed to establish some basic characteristics of Atlantic bluefin tuna sperm from captive mature males, treated or untreated by gonadotropin releasing hormones agonist (GnRHa). Intratesticular milt was collected from treated and untreated fish (mean weight +/- SD: 122.9 +/- 29.2 kg, n = 21). There was no significant effect of GnRHa treatment on GSI (1.33 +/- 0.70%, n = 21) or on sperm concentration (3.8 +/- 1.3 x 1010 spermatozoa ml-1, n = 21) estimated by optical density at 260 nm. Similarly, the percentage of motile spermatozoa measured at 30 s post activation: activating medium (AM seawater containing 10 mg ml-1 BSA) was not significantly different between control and GnRHa implanted males. On the other hand, a significantly...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00015/12645/9722.pdf

Etat d'avancement des travaux sur les poissons tropicaux en Martinique
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Soletchnik, Patrick; Thouard, Emmanuel; Galllet De Saint-aurin, D.; Suquet, Marc; Hurtaud, P.; Mesdouze, J. P.
Ce document se compose de 3 publications :  - overview of the studies conducted on the finfish, Palometa (Trachinotus goodei), in Martinique,  - spawning of palometa (Trachinotus goodei), in capacity, - spawning of yellowtail snapper (Ocyurus chrysurus ; Bloch, 1791) in capacity.  Cette dernière publication, résume l'essentiel des informations obtenues sur la sarde et présentées d'une façon exhaustive dans le document scientifique n°9.  Les 2 premières publications de ce document, consacrées à la carangue, font état des derniers résultats acquis sur cette espèce.
Tipo: Text
Palavras-chave: Aquaculture; Poissons tropicaux; Elevage; Maturation; Reproduction; Trachinotus goodei; Ocyurus chrysurus; Martinique; Aquaculture; Tropical finfishs; Rearing; Maturation; Reproduction; Trachinotus goodei; Ocyurus chrysurus; Martinique.
Ano: 1988
URL: http://archimer.ifremer.fr/doc/00131/24262/22258.pdf

Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boulais, Myrina; Soudant, Philippe; Le Goic, Nelly; Quere, Claudie; Boudry, Pierre; Suquet, Marc.
In the Pacific oyster, spermatozoa are characterized by a remarkably long movement phase (i.e., over 24 h) sustained by a capacity to maintain intracellular ATP level. To gain information on oxidative phosphorylation (OXPHOS) functionality during the motility phase of Pacific oyster spermatozoa, we studied 1) changes in spermatozoal mitochondrial activity, that is, mitochondrial membrane potential (MMP), and intracellular ATP content in relation to motion parameters and 2) the involvement of OXPHOS for spermatozoal movement using carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The percentage of motile spermatozoa decreased over a 24 h movement period. MMP increased steadily during the first 9 h of the movement phase and was subsequently maintained at a...
Tipo: Text
Palavras-chave: Crassostrea gigas; Intracellular ATP content; Mitochondrial membrane potential; Oxidative phosphorylation; Sperm motility.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00303/41376/40666.pdf

Transcriptomic features of Pecten maximus oocyte quality and maturation
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pauletto, Marianna; Milan, Massimo; Huvet, Arnaud; Corporeau, Charlotte; Suquet, Marc; Planas, Josep V.; Moreira, Rebeca; Figueras, Antonio; Novoa, Beatriz; Patarnello, Tomaso; Bargelloni, Luca.
The king scallop Pecten maximus is a high valuable species of great interest in Europe for both fishery and aquaculture. Notably, there has been an increased investment to produce seed for enhancement programmes of wild scallop populations. However, hatchery production is a relatively new industry and it is still underdeveloped. Major hurdles are spawning control and gamete quality. In the present study, a total of 14 scallops were sampled in the bay of Brest (Brittany, France) to compare transcriptomic profiles of mature oocytes collected by spawning induction or by stripping. To reach such a goal, a microarray analysis was performed by using a custom 8x60K oligonucleotide microarray representing 45,488 unique scallop contigs. First we identified genes...
Tipo: Text
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00374/48484/48751.pdf

Caractéristiques biologiques des gamètes et larves d'huître plate européenne (Ostrea edulis): données préliminaires
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Queau, Isabelle; Le Grand, Jacqueline; Ratiskol, Dominique; Pouvreau, Stephane.
      The biology of gametes and larvae in brooding mollusc species remains little explored. The aim of this study is to present the results obtained in 2016, on biological characteristics of gametes and larvae of European flat oyster.       Measurements were carried out on 50 oysters, collected by divers in the Rade de Brest (Finistère, France), during the spawning period of this species. Gonad pH is acidic (6.58±0.54). After the dissociation of spermatozeugmata, an acellular structure to which the spermatozoa are clustered, the percentage of motile spermatozoa ranged from 20 to 34% and the Velocity of the Average Path from 70 to 85µm/s. Spermatozeugmata were shed after serotonin injection. Oocytes (mean diameter: 129.4±19.1µm) were rarely observed (2...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00383/49390/49803.pdf

Evaluation of fish sperm quality
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Fauvel, Christian; Suquet, Marc; Cosson, J.
P&gt;The development of cheaper technical tools and the availability of well established assay kits provide today the spermatologists with several devices for objective and quantifying assessment of sperm quality. The present paper reviews modern methods on sperm concentration assessment, morphometry, motility processes through flagellum studies, motility objective assessment by computer assisted sperm analysis (CASA), sperm integrity and energetic state measurements.
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00015/12642/13921.pdf

Short-term storage and cryopreservation of turbot (Scophthalmus maximus) sperm
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Chereguini, Olvido; Cal, Rosa Maria; Dreanno, Catherine; Ogier De Baulny, Bénédicte; Suquet, Marc; Maisse, Gérard.
Short-term storage over several days as well as cryopreservation of turbot (Scophthalmus maximus) sperm were studied. Two extenders, Ringer 200 and artificial seminal liquid (ASL2), are suggested for semen collection in order to avoid the deleterious effect of urine contamination, and for the purpose of short-term storage between 0 and 15 degrees C. Oxygen atmosphere is not suitable for turbot sperm storage. Turbot spermatozoa undergo cryopreservation with a high rate of success especially in a sucrose solution with 10% dimethyl sulfoxide (DMSO) and 10% egg yolk.
Tipo: Text
Palavras-chave: Turbot; Sperm; Storage; Cryopreservation.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00176/28724/27173.pdf

Mise au point d'outils de gestion des gamètes mâles d'huîtres creuses Crassostrea gigas diploïdes et tétraploïdes à des fins de conservation et de diffusion
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Brizard, Raphael; Boudry, Pierre; Haffray, Patrick; Labbe, C.; Suquet, Marc; Robert, Rene; Masse, G.; Pepin, Jean-francois; Saulnier, Denis.
In order to improve oyster culture, many research programs try and propose new methods aiming at improving genetically the product performances and quality (selection, polyploidisation). As for terrestrial animal production, the continuity of the different works as well as the spreading of the genetical progress would be made easier by the development of conservation techniques of the selected genotypes. Cryopreservation, which is often used in selection programs of terrestrial animals or fish farming species, is not always available for bivalves. This technique would enable the conservation of the gametes of individuals selected for their high growth potential, their resistance to diseases and/or their quality for a smaller cost and less risks than the...
Tipo: Text
Palavras-chave: Tetraploïde; Diploïde; Concervation; Spermatozoides; Cryoconservation; Génétique; Crassostrea gigas; Huîtres.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3339.pdf

Effect of stripping frequency on turbot spawn quality.
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Fauvel, Christian; Omnes, Marie-helene; Suquet, Marc; Normant, Yvon.
In production operations the application of daily examination of each female of broodstock should be a determinant progress in hatchery management in terms of fry output.  The eggs expelled shortly after ovulation by means of a systematic fivefold a week stripping attempt of females, presented a high viability rate (72%) compared to that of eggs collected twice a week (46%) whereas the treatments did not have any effect on female fecundity.  Increase of the stripping rhythm brought the hatching rates and broodstock fertilities respectively from 11% to 35% and from 20,000 to 65,000 larvae/kg of spawning female. The enhancement of egg quality and of the resulting larva production is discussed.
Tipo: Text
Ano: 1991
URL: http://archimer.ifremer.fr/doc/00170/28129/26346.pdf

Cryopreservation of Pacific oyster ( Crassostrea gigas ) larvae: Revisiting the practical limitations and scaling up the procedure for application to hatchery
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Labbe, Catherine; Haffray, Pierrick; Mingant, Christian; Quittet, Benjamin; Diss, Blandine; Tervit, H. Robin; Adams, Serean L.; Rimond, Flore; Suquet, Marc.
Pacific oyster Crassostrea gigas is one major species for aquaculture, and the development of breeding programs and the need for preservation of wild stock genetic resources prompted the need for larvae cryopreservation. The objective of the present study was to choose the most reliable protocol from several existing publications, to test its biological and practical limitations, and to adapt it to hatchery conditions. The selected protocol was characterized by a very slow freezing rate without seeding, and by the use of ethylene glycol and sucrose as cryoprotectant. The best survivals after thawing and rearing up to 48 h post fertilization (hpf) were obtained with larvae that were frozen at late trochophore (20 hpf) and early-D (24 hpf) stages. Increasing...
Tipo: Text
Palavras-chave: Oyster; Cryobanking; D stage; Ethylene glycol; High throughput; Hatchery.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00423/53460/54354.pdf

Application of staining techniques to improve the viability assessment of turbot (Psetta maxima) ova
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Omnes, Marie-helene; Dorange, Germaine; Suquet, Marc; Normant, Yvon.
Staining-dye procedures were tested on the turbot ovule in order to develop a rapid technique for determining ovule viability. Neutral red and Trypan blue dyes provided better assessment of the proportion of viable or dead female gametes when inducing fertilisation.
Tipo: Text
Ano: 1999
URL: http://archimer.ifremer.fr/doc/00002/11323/7860.pdf

Sperm features in turbot (Scophthalmus maximus): a comparison with other freshwater and marine fish species
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Billard, Roland; Cosson, Jacky; Dorange, Germaine; Chauvaud, Laurent; Mugnier, Chantal; Fauvel, Christian.
This paper presents some features of turbot sperm and compares them with data reported in the literature on other freshwater and marine fish. In captivity, turbot spermiation period lasts 6 months. When compared to other fish species, turbot males are characterized by low values of maximum gonadosomatic index (0.6-0.8%) and are poor sperm producers in terms of volume (0.2-2.2 ml), concentration (0.7-11.0 x 10(9) spermatozoa/ml) and total sperm number released per stripping (0.2-12.0 x 10(9) spermatozoa). Compared to most freshwater species, the duration of cell movement is long (1-17 min). The spermatozoon of turbot can be considered to be of a primitive type. A very high protein content (8.8 mg/ml) is found in the seminal fluid of turbot. Increasing...
Tipo: Text
Palavras-chave: Scophthalmus maximus; Turbot; Biologie du sperme; Liquide séminal; Mobilité du spermatozoïde; Morphologie du spermatozoïde; Poisson marin; Scophthalmus maximus; Turbot; Sperm biology; Seminal fluid; Sperm motility; Sperm morphology; Marine fish.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00188/29893/28334.pdf

Optimizing sperm cryopreservation in great scallop (Pecten maximus)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Malo, Florent; Gourtay, Clemence; Ratiskol, Dominique; Riobe, Marion; Le Grand, Jacqueline; Queau, Isabelle; Mingant, Christian.
A basic cryopreservation protocol for great scallop sperm was recently published, with which thawed sperm survival remained limited to 25% of control values (Suquet et al. 2014). We designed here a set of five experiments to test ways of improving thawed sperm survival and storage by studying the effects of (1) cryoprotectant concentration; (2) sugar incorporation in the extender; (3) egg yolk addition to the extender; (4) sperm dilution rate; and (5) storage capacity of thawed sperm at 4 degrees C. A sixth experiment was then performed to compare rearing performances of larvae produced using thawed sperm from the optimal protocol defined in the present study with those produced using fresh sperm. During the first experiment, a significantly higher...
Tipo: Text
Palavras-chave: Pecten maximus; Mollusc; Sperm; Cryopreservation; Larval rearing.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00359/47033/46946.pdf

Anaesthesia and gonad sampling in the European flat oyster (Ostrea edulis)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Gonzalez, Ricardo; Lebrun, Luc; Queau, Isabelle; Mingant, Christian; Robert, Rene.
Controlled reproduction of the European flat oyster requires the development of tools adapted to this species, including a practical anaesthesia and gonad sampling protocol to facilitate sex determination and the verification of gametogenesis. Three replicate groups of 10 oysters (mean weight +/- SD: 29.9 +/- 8.5 g) were anaesthetised in 5 L containers using magnesium chloride designed either for laboratory (Flucka (R)) or agricultural (Dead Sea Work (R): DSW) use, at two concentrations (50 or 72 g L-1). No significant differences were observed in the percentages of oysters anaesthetised or subsequent oyster mortality with the different anaesthetics or concentrations, but increasing water temperature from 14.9 to 18.8 degrees C significantly increased the...
Tipo: Text
Palavras-chave: Oyster; Ostrea edulis; Anaesthesia; Magnesium chloride; Gonad sampling.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00018/12907/9876.pdf

Compte rendu final du projet Triplofimer : Fiabilité et qualité de la production de naissains triploïdes d'huître creuse (Crassostrea gigas)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Agogue, Julie; Brizard, Raphael; Haffray, Pierrick; Labbe, Catherine; Le Coz, Jean-rene; Pepin, Jean-francois; Rault, Paul; Saulnier, Denis.
Ce rapport fait état d'une étude réalisée au laboratoire de Génétique et Pathologie de l'IFREMER dans le cadre de l'analyse d'épisodes de mortalité anormale survenue en écloserie privée de crevettes pénéides. Plusieurs hypothèses ont été avancées. La présence de Vibrio harveyi a amené à constituer une collection de 40 souches à partir desquelles une identification bactériologique a été effectuée. Elle montre en effet une dominance de souches appartenant à un groupe homogène de Vibrio harveyi. La technique de ribotypage (digestion d'ADN génomique, transfert de Southem-blot et hybridation avec une sonde spécifique) est alors mise en place. Plusieurs difficultés techniques ont ralenti l'analyse et n'ont donc pas permis d'avoir des résultats assez avancés pour...
Tipo: Text
Palavras-chave: Huître creuse; Crassostrea gigas; Tétraploïdes; Sperme; Conservation; Diagnostic zoo-sanitaire; Flore bactérienne; Herpès virus.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/00304/41554/40762.pdf

Description of hake (Merlucius merlucius) spermatozoa: flagellar wave characteristics and motility parameters in various situations
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Cosson, J.; Groison,; Fauvel, Christian; Suquet, Marc.
P&gt;The objective of this study was to complement published hake (Merlucius merlucius) sperm motility characteristics by obtention of data more specifically dependent on flagella: beat frequency, flagellar wave amplitude, wave length, number of waves along the length of the flagellum and curvature of the wave. The changes of these parameters are described in relation to the time period elapsed since activation are described using high resolution video images of flagella while quantifying the progression during the swimming period from the activity initiation to full cessation of movement several minutes later. The main characteristic of the swimming period of hake spermatozoa shows a progressive decrease of forward displacement resulting from a cumulative...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00015/12643/9586.pdf

Effect of sampling location, release technique and time after activation on the movement characteristics of scallop (Pecten maximus) sperm
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Quere, Claudie; Mingant, Christian; Lebrun, Luc; Ratiskol, Dominique; Miner, Philippe; Cosson, Jacky.
Sperm characteristics of scallops have not been well described in the scientific literature. The effects of sperm release technique (thermal shock versus serotonin injection), of sperm collection technique (testis sampling versus serotonin injection), of sperm sampling location along the genital tract, of in vitro sperm maturation, and of time post activation on scallop sperm characteristics were assessed in the present work. Whatever sperm release technique used, no significant differences were observed regarding the percentage of motile spermatozoa and the velocity of the average path (YAP). Compared to testicular sperm, a higher percentage of motile spermatozoa, YAP and intracellular adenosine triphosphate (ATP) content were observed for sperm shed...
Tipo: Text
Palavras-chave: Spermatozoa; CASA; Maturation; Sperm motility; Bivalve; Pecten maximus.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00157/26852/24983.pdf

Gestion des populations de reproducteurs de loup (Dicentrarchus labrax), placées en conditions de pontes naturelles ou décalées
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc.
Tipo: Text
Ano: 1987
URL: http://archimer.ifremer.fr/doc/00355/46626/46442.pdf

Pollack (Pollachius pollachius): Acquisition of biologic data in a breeding purpose
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Buchet, Vincent; Suquet, Marc; Omnes, Marie-helene; Petton, Bruno; Normant, Yvon; Severe, Armelle; Quemener, Loic; Barone, Herve; Fauvel, Christian; Gaignon, Jean-louis.
The fishing of pollack which concerns essentially, France, United Kingdom and Norway, and at a lower level Ireland and Denmark, is situated between 10 and 15000 tons a year. In 1996, France was the first producer of Pollack. Since 1980, a decrease of production is observed in Europe. In France, landing reached 3000t. in 1998 (Suquet, 2001). However, the demand remaining strong, aquaculture could be a solution to satisfy the deficit between the French production and the domestic demand.
Tipo: Text
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00170/28144/26365.pdf

La domestication des poissons : le cas du thon rouge
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Fauvel, Christian; Suquet, Marc.
Bluefin tuna shows adaptative physiology features allowing life in a wide range of physical and climatic parameters like temperature, salinity and hydrostatic pressure, hence the species fulfil the main basic requirements for progressive domestication and rearing in captivity. The high mortalities recorded after handling and the losses observed after abrupt variations of environment in the cages, emphasised the extreme sensitivity to stress of tuna. As consequences, the progresses about the control of tuna biological cycle are very slow due to limited handling possibilities and tuna aquaculture is restricted to fattening of wild fish. At this time, bluefin tuna production progresses without domestication of the species.
Tipo: Text
Ano: 2004
URL: http://archimer.ifremer.fr/doc/00272/38283/36473.pdf

Setting tools for the early assessment of the quality of thawed Pacific oyster (Crassostrea gigas) D-larvae
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Le Mercier, Alain; Rimond, Flore; Mingant, Christian; Haffray, Pierrick; Labbe, C.
Parameters used to assess the survival of larvae after cryopreservation generally misestimate the damages that prevent larval development. The objectives of the present study were to 1) define the reliability of the survival rate, assessed at 2 and 7 days post fertilization, to estimate Pacific oyster larval quality after thawing, and 2) select complementary tools allowing an early and reliable estimation of their quality. Oyster larvae were reared for 25 h after fertilization at 19 degrees C and cryopreserved at early D-stage. Then, thawed larvae were incubated in 2-L beakers. At 2 days post fertilization, the survival rate of thawed Pacific oyster larvae was lower than that of fresh larvae for only one experiment (Experiment 3) among the four identical...
Tipo: Text
Palavras-chave: ASMA; CASA; Crassostrea gigas; Cryopreservation; D-larvae.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00088/19934/17761.pdf

Sperm motility in European hake, Merluccius merluccius, and characterization of its spermatozoa concentration and volume, spermatocrit, osmolality and pH
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Groison, Anne-laure; Suquet, Marc; Cosson, Jacky; Mayer, Ian; Severe, Armelle; Bouquet, Jean-marie; Geffen, Audrey J.; Utne-palm, Anne Christine; Kjesbu, Olav Sigurd.
Due to stock declines there is a growing interest in farming of European hake Merluccius merluccius. Thus, knowledge of its sperm biology is of importance not only for purposes of broodstock management, but also for the development of sperm preservation techniques. Hake sperm were collected from mature males caught during the summer-early autumn waters off western Norway and during the winter-early spring in the Bay of Biscay (France). Sperm quality characteristics were assessed after storage at 4 degrees C for 25 +/- 14 h. Average (+/- SD) values for Norwegian and French samples respectively were (i) sperm volume: 3.9 +/- 4.0 and 2.6 +/- 4.0 ml; (ii) spermatozoa concentration: 6.6 +/- 3.2 and 13.9 +/- 5.1 x 10(9) spermatozoa/ml; (iii) spermatocrit: 80.2...
Tipo: Text
Palavras-chave: Cryopreservation; Merluccius merluccius; Osmolality; PH; Sperm motility; Spermatocrit.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00003/11393/8021.pdf

Effect of age and environmental conditions on the movement characteristics of Pacific oyster (Crassostrea gigas) trochophores
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Rimond, Flore; Cosson, J.; Wilson-leedy, J.; Lebrun, Luc; Queau, Isabelle; Mingant, Christian; Fauvel, Christian.
The movement characteristics of Pacific oyster trochophores have received very little coverage in the scientific literature. Described here are not only changes in the swimming characteristics of Crassostrea gigas trochophores (size: 53-77 mu m) in relation to time after fertilization and to inter-female variation but also the effects of salinity and pH on movement characteristics. The percentage of motile trochophores was measured on images obtained through a dissecting microscope and the Velocity Average Path (VAP) was assessed using a Computer Assisted Sperm Analysis (CASA) system. At 20 degrees C, the first movements of the trochophores were observed at 6.5 h post-fertilization. The mean (+/- SD) percentage of swimming trochophores and the VAP observed...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00157/26786/24940.pdf

Egg production in the turbot (Scophthalmus maximus) in relation to broodstock management
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Omnes, Marie-helene; Normant, Yvon; Suquet, Marc; Fauvel, Christian.
Egg production data from individual hand stripped female turbot were collected and analysed over several consecutive spawning seasons, on natural or shifted conditions. Egg quantity, egg viability and number of spawns were examined from one spawning season to the other, and inside the spawning season. These parameters seemed to increase from a year to the next one, except for the oldest fishes. Spawns were spread over a three-month period and occurred at the same time in the period in successive seasons for most females. Egg production seemed related to the feeding level and the earliness of spawning season appeared depended on the growth rate. Without sand substrate in tanks, injuries could increase therefore no significant differences were observed in...
Tipo: Text
Ano: 1992
URL: http://archimer.ifremer.fr/doc/00199/31018/29423.pdf

Cryopreservation of sperm in marine fish
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Dreanno, Catherine; Fauvel, Christian; Cosson, J; Billard, R.
Since the first work of Blaxter in 1953, fish sperm cryopreservation has been attempted on about 30 marine species. The present paper reviews the techniques used and the results published in these species. Particular attention is paid to the handling procedure of sperm before freezing, the problems of semen ageing and semen contamination with urine. The quality of frozen-thawed semen was evaluated using previously standardized biotests, such as a two-step motility activation technique adapted for the different species and fertilization assays using a discriminating insemination technique. Most extenders used in marine fish are saline or sugar solutions. From the investigated cryoprotectants, dimethyl sulphoxide (DMSO) generally leads to the best results....
Tipo: Text
Palavras-chave: DMSO; Insemination; Fertilization; Cryopreservation; Fish sperm.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-611.pdf

Effect of temperature, volume of ova batches, and addition of a diluent, an antibiotic, oxygen and a protein inhibitor on short-term storage capacities of turbot, Psetta maxima, ova
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Chereguini, Olvido; Omnes, Marie-helene; Rasines, Inmaculada; Normant, Yvon; Souto, Isabel; Quemener, Loic.
The effect of different parameters on shea-term storage capacity of turbot ova was assessed over a 45-h period after ova collection for fertilization rates and over a 9-h period after ova collection for hatching rates. Increasing the volume of ova sampling from 0.5 to 2.5 mt, as well as adding an antibiotic-antimicotic solution or oxygen did not significantly change the storage capacity of ova. Regarding the hatching rates, a higher storage ability was recorded at 8 and 13 degrees C, compared to 3 degrees C. The mean composition of the ovarian fluid was determined (n = 57 spawns). Use of a diluent mimicking the ovarian fluid significantly decreased the storage ability as assessed by the fertilization rates but did not modify the hatching rates. Diluting...
Tipo: Text
Palavras-chave: Psetta maxima; Turbot; Calcium; Ovarian fluid; Short term storage; Ova; Fish; Psetta maxima; Turbot; Calcium; Liquide ovarien; Conservation à court terme; Ovules; Poisson.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/publication-857.pdf

Marine fish spermatozoa: racing ephemeral swimmers
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Cosson, J; Groison, A; Suquet, Marc; Fauvel, Christian; Dreanno, Catherine; Billard, R.
After along period of spermatogenesis (several weeks to months), marine fish spermatozoa are delivered at male spawning in seawater (SW) at the same time as ova. In some fish species, as the ova micropyle closes quickly after release, these minute unicells, the spermatozoa, have to accomplish their task of reaching the micropyle within a very brief period (several seconds to minutes), for delivery of the haploid male genetic information to the ova. To achieve this goal, their high-performance motile equipment, the flagellum, must fully activate immediately on contact with the SW and then propel the sperm cell at an unusually high initial velocity. The cost of such 'hyperactivity' is a very rapid consumption of intracellular ATP that outstrips the supply....
Tipo: Text
Palavras-chave: Cell activation; Osmolality; Ions; Cell energetics; Motility; Spermatozoon; Marine fish.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-6967.pdf

Seasonal variation of sperm quality in Pacific oyster (Crassostrea gigas)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Malo, Florent; Queau, Isabelle; Ratiskol, Dominique; Quere, Claudie; Le Grand, Jacqueline; Fauvel, Christian.
Seasonal changes of sperm quality which can affect sperm biological parameters throughout the breeding period, have been little studied in mollusc species. Controlling gamete quality would aid the management of gametes in hatcheries and the development of selection programs. The aim of the present study was to describe the changes in sperm quality of wild Pacific oysters through the spawning season by comparing sperm parameters at the beginning (May), middle (July) and end (October) of this period using a panel of bio-descriptors. These parameters were studied over the 2014 breeding season based on shed sperm collected after serotonin injection of wild breeders. A significantly higher percentage of motile sperm was observed at the end of the spawning...
Tipo: Text
Palavras-chave: Crassostrea gigas; Sperm quality; Sperm ageing; Season; Motility.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00346/45690/45310.pdf

Individual monitoring of gonad development in the European flat oyster Ostrea edulis by in vivo magnetic resonance imaging
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Davenel, Armel; Gonzalez, Ricardo; Suquet, Marc; Quellec, Stephane; Robert, Rene.
Previous studies have shown that magnetic resonance imaging is a very appropriate non-invasive technique for quantifying the growth of somatic and gonadic tissues and sex determination in the Pacific oyster, Crassostrea gigas. Despite a thinner gonad, harder to distinguish than that of the Pacific oyster, we showed in this study that it was possible to distinguish and quantify the development of the gonad of the European flat oyster, Ostrea edulis, using a 3D MRI sequence with a suitable finer resolution. Compared to T2-weighted images, theoretically the most appropriate for good anatomical description, T1-weighted images were more suited to gonad observation. The gonad development was quantified by the number of voxels with higher grey level. Larvae were...
Tipo: Text
Palavras-chave: Oyster; Ostrea edulis; Gonad development; Magnetic resonance imaging.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00014/12508/9363.pdf

Setting of a procedure for experimental fertilisation of Pacific oyster (Crassostrea gigas) oocytes
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Song, Yun-peng; Suquet, Marc; Queau, Isabelle; Lebrun, Luc.
The quality of oyster spermatozoa has become an issue for modern aquaculture. However, reliable protocols used to assess gamete quality are lacking. The aim of this work is to define a standardised experimental fertilisation protocol for Pacific oyster oocytes. Six experiments have been carried out in this study. The optimal conditions for Pacific oyster experimental fertilisation have been defined: (1) oocytes can be conserved in seawater for at least 4 h before fertilisation. (2) oocyte concentration: 100-1000 ml(-1) seawater, (3) fertilisation volume: 10-100 nil, (4) spermatozoa: oocyte ratio: 400 to maintain discriminating conditions, (5) gamete contact time: longer than 10 mill and (6) fertilisation and incubation temperatures: both 19 degrees C. The...
Tipo: Text
Palavras-chave: Crassostrea gigas; Pacific oyster; Experimental; Fertilisation.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6231.pdf

Cod (Gadus morhua) rearing attempts in France
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Omnes, Marie-helene; Normant, Yvon; Petton, Bruno; Severe, Armelle; Fauvel, Christian; Barone, Herve; Quemener, Loic; Buchet, Vincent; Pasco, Laurent; Menard, E; Gaignon, Jean-louis.
Because of its rapid growth, its good reputation and the promising aquaculture experiences conducted in Norway and Scotland, cod is considered as a good candidate for cold water aquaculture. The aim of this paper is to present the work carried out in France since 1999: the selection of cod as a promising candidate for aquaculture and the assessment of its rearing performances in this environment.
Tipo: Text
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00170/28142/26362.pdf

Sexual dimorphism of drumming muscles in European hake (Merluccius merluccius)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Groison, Anne-laure; Kjesbu, Olav S.; Suquet, Marc.
Dissections of mature and non-mature European hake males and females (N=142) collected in waters off the western coast of Norway and in the Bay of Biscay (France) in 2004-2006 demonstrate for the first time that this gadoid species contains drumming muscles. There were differences in drumming muscles weight with body length, sex and maturity stage. This study shows that the difference between females and males is primarily manifested during the spawning season, seen both in the French and Norwegian samples. For the mature females the drumming muscles dry weight increases only slightly, if at all, with increase in total length. For mature males there is a corresponding rapid increase. There does not seem to be any consistent difference between the average...
Tipo: Text
Palavras-chave: Drumming muscles; Merluccius merluccius; Sexual dimorphism; Sound production; Spawning.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00036/14681/11983.pdf

CRYOAQUA. Centralisation de la cryoconservation et du stockage des ressources génétiques aquacoles françaises
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Labbe, Catherine; Suquet, Marc; Michel, Gildas; Duclos, Delphine; Lemarchand, Alain; Le Hellec, Catherine; Vandeputte, Marc; Quittet, Benjamin; Goardon, Lionel; Pincent, Cedric; Haffray, Pierrick.
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00099/20979/18602.pdf

Slow-release GnRHa therapy prevented atresia during vitellogenesis and induced ovulation of captive wreckfish (Polyprion americanus)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Fauvel, Christian; Suquet, Marc; Severe, Armelle; Mylonas, Constantinos C.; Papandroulakis, Nikos.
Tipo: Text
Ano: 2007
URL: http://archimer.ifremer.fr/doc/00346/45750/45392.pdf

Slow-release GnRHa treatment prevented atresia during vitellogenesis and induced ovulation of captive wreckfish (Polyprion americanus)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Fauvel, Christian; Suquet, Marc; Severe, Armelle; Mylonas, Constantinos C.; Papandroulakis, Nikos.
The potential of wreckfish (Polyprion americanus) to breed in captivity was examined. Ovarian activity varied seasonally, with vitellogenesis beginning in November and ending in May. Slow-release implant with gonadotropin-releasing hormone agonist (GnRHa) prevented atresia, and both GnRHa injections or implants at the end of vitellogenesis triggered ovulation after 7 days. Spontaneous spawning was obtained only in one female, and the few eggs produced were of poor viability. Manual stripping and artificial fertilization produced viable gametes and embryos. Therefore, present captivity conditions inhibited final oocyte maturation (FOM) and spawning, but not vitellogenesis and spermatogenesis. GnRHa treatments stimulate FOM and ovulation of viable eggs.
Tipo: Text
Palavras-chave: Aquaculture; Polyprion americanus; Wreckfish; GnRHa; Gametogenesis.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-6554.pdf

Cryobanking of aquatic species
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Martinez-paramo, Sonia; Horvath, Akos; Labbe, Catherine; Zhang, Tiantian; Robles, Vanesa; Herraez, Paz; Suquet, Marc; Adams, Serean; Viveiros, Ana; Tiersch, Terrence R.; Cabrita, Elsa.
This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of...
Tipo: Text
Palavras-chave: Cryobanking; Aquatic animals; Fish gametes; Germplasm.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00341/45172/44567.pdf

Hatchery-scale trials using cryopreserved spermatozoa of black-lip pearl oyster, Pinctada margaritifera
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Hui, Belinda; Vonau, Vincent; Moriceau, Jacques; Tetumu, Roger; Vanaa, Vincent; Demoy-schneider, Marina; Suquet, Marc; Le Moullac, Gilles.
Cryopreservation is a valuable tool for genetic improvement programs. Several bivalve mollusc species have already been the subject of such programs and the Tahitian black pearl oyster industry is now planning the development of selective breeding for desirable traits in Pinctada margaritifera. The ability to cryopreserve spermatozoa would, therefore, offer significant benefits to the cultured black pearl industry.  Spermatozoa were cryopreserved with CPA 0.7 M trehalose in 0.8 M Me2SO and a two-step freezing process was used: straws were first maintained in nitrogen vapour for 10 minutes, then directly plunged into liquid nitrogen and stored for one week before use. The viability of thawed sperm was 23 % lower than that of fresh sperm. When using thawed...
Tipo: Text
Palavras-chave: Cryopreservation; Spermatozoa; Fertility; Larval rearing; Pearl oyster; Pinctada margaritifera.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00040/15098/12446.pdf

Biological characteristics of sperm in European flat oyster (     Ostrea edulis     )
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Pouvreau, Stephane; Queau, Isabelle; Boulais, Myrina; Le Grand, Jacqueline; Ratiskol, Dominique; Cosson, Jacky.
n brooding mollusc species that incubate larvae before their release in seawater, the biology of gametes remains poorly explored. The present study describes some biological characteristics of sperm of the European flat oyster, a native species that has been over-exploited in the past and is nowadays classified as an endangered species in Europe. Flat oysters were collected by divers in the Rade de Brest (Finistère, France), during its natural reproduction period. Gonadal pH is acidic (6.31 ± 0.10). Spermatozoa are clustered in spermatozeugmata, an acellular structure in which the sperm heads are embedded. After their transfer in seawater, spermatozeugmata have a mean diameter of 64 ± 3 μm and they release free spermatozoa for a mean duration of...
Tipo: Text
Palavras-chave: Oyster; Ostrea edulis; Brooding species; Sperm; Spermatozeugmata; Endangered species.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00454/56539/58245.pdf

Long-term effects of the cryopreservation of turbot (Psetta maxima) spermatozoa
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Dreanno, Catherine; Petton, Bruno; Normant, Yvon; Omnes, Marie-helene; Billard, Roland.
The survival of turbot eggs and the rearing capacities of larvae stemmed from artificial fertilization practices using frozen-thawed spermatozoa were evaluated. Furthermore, the viability of sperm samples stored during a 9 month period in liquid nitrogen was assessed. No significant difference in the fertilization rate, hatching rate, survival and wet weight of 10-day old larvae were observed using fresh or frozen-thawed spermatozoa. The motility recorded at 10 s and 60 s post-activation and the fertilization capacity of frozen-thawed spermatozoa were not significantly decreased during a 9 month storage period in liquid nitrogen. These results confirm the high quality of the turbot spermatozoa stemmed from the cryopreservation process, allowing their use...
Tipo: Text
Palavras-chave: Turbot; Psetta maxima; Sperm; Cryopreservation; Hatching.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00176/28721/27176.pdf

Effect of water temperature on individual reproductive activity of pollack (Pollachius pollachius)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Normant, Yvon; Gaignon, Jean-louis; Quemener, Loic; Fauvel, Christian.
The effects of water temperature on pollack (Pollachius pollachius) spawning features were individually assessed. During the spawning period, nine fish sets, each composed of one female and two males (mean body weight: 2.5+/-1.0 kg), were placed in small-volume tanks (2 m(3)). Fish sets were held at 8, 10, or 12 degreesC (n=3 for each temperature).   Three females released eggs at 8 and 10 degreesC, while only two spawned at 12 degreesC. One hundred thirty-one individual spawns were collected from the eight spawning fish sets. The number of spawns collected per female was significantly lower at 12 degreesC (3.0+/-3.6), compared to 10 degreesC (17.3+/-10.1) and 8 degreesC (23.3+/-2.5). Egg number (eggs kg(-1) BW) was significantly lowered at 12 degreesC...
Tipo: Text
Palavras-chave: Egg quality; Temperature; Reproduction; Pollachius pollachius; Pollack; Gadoids.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-470.pdf

Changes in Atlantic cod (Gadus morhua L.) sperm quality during the spawning season
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rouxel, Catherine; Suquet, Marc; Cosson, J; Severe, Armelle; Quemener, Loic; Fauvel, Christian.
The biology of cod reproduction is well described in the scientific literature. However, sperm biology and spermatozoa management are poorly studied in this species. Because of its recent farming expansion, a better knowledge of cod gametes is becoming especially useful. This work aimed at establishing tools to study sperm biology in cod, and also investigated the existence of changes in cod sperm quality during the spawning period. We showed that sperm concentration could be assessed using spectrophotometry at 260 nm. Sperm motility significantly decreased after a 168-h storage at 4 degrees C. A 1:9 dilution of sperm in a non-activating medium (1/3 seawater and 2/3 freshwater, osmotic pressure: 360 mOsm kg(-1)) improved sperm storage. Sperm concentration,...
Tipo: Text
Palavras-chave: Spermatozoa; Quality; Ageing; Sperm; Cod; Gadus morhua.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3829.pdf

First rearing attempts of pollack, Pollachius pollachius
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Petton, Bruno; Normant, Yvon; Dosdat, Antoine; Gaignon, Jean-louis.
Tipo: Text
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00188/29888/28339.pdf

Cryopreservation of sea bass (Dicentrarchus labrax) spermatozoa in experimental and production simulating conditions
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Fauvel, Christian; Suquet, Marc; Dreanno, Catherine; Zonno, Vincenzo; Menu, Bruno.
A sperm cryopreservation protocol adapted from turbot, was tested on sea bass using either 250-mu L straws or 1.5-mL cryovials. A dilution to 1/3 in Mounib's extender and a cooling rate of 65 degrees C.min(-1) allowed frozen sperm to recover an initial motility similar to that of fresh sperm at thawing; however, significant differences in motility (P &lt; 0.001, n = 10 fish semen) were observed at further post-activation times, the motility decrease being faster in thawed sperm. At the experimental scale, triplicate inseminations of 2-mL aliquots (approximately 2 000 eggs) showed a significant fertility decay of thawed sperm compared to that of fresh sperm (P &lt; 0.01, n = 12 fish semen) when a discriminating 35.10(3) spermatozoa to egg ratio was applied....
Tipo: Text
Palavras-chave: Dicentrarchus labrax; Sea bass; Cryobank; Insemination; Spermatozoa; Cryopreservation; Dicentrarchus labrax; Loup; Banque de sperme; Insémination; Cryoconservation; Cryoconservation.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/publication-866.pdf

Motility characteristics of spermatozoa in cod (Gadus morhua) and hake (Merluccius merluccius)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Cosson, Jacky; Groison, Anne-laure; Suquet, Marc; Fauvel, Christian.
As in many marine fish, motility is triggered by osmotic pressure (OP) in cod and hake spermatozoa and motility ranges several minutes with a high velocity (above 50 mu m/s) period restricted to 100 s. A decrease in the flagellar beat frequency (BF) is partly responsible of this briefness. Detailed observations of the flagella during the motility period showed: 1) sea water provokes OP damages (blebs) which impair the correct wave propagation 2) waves become restricted to the proximal flagellum and tip becomes devoid of wave 3) the wave amplitude decreases. The combination of these factors drastically limits to the earliest period of motility the ability for spermatozoa to efficiently progress towards egg for fertilization.
Tipo: Text
Palavras-chave: Osmotic pressure; Sperm motility; Spermatozoa; Hake; Cod.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-6552.pdf

Marathon vs sprint racers: an adaptation of sperm characteristics to the reproductive strategy of Pacific oyster, turbot and seabass
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Cosson, J.; Donval, Anne; Labbe, C.; Boulais, Myrina; Haffray, Pierrick; Bernard, Ismael; Fauvel, Christian.
This work assesses the present knowledge on Pacific oyster sperm biology in comparison to two marine fish species (turbot and seabass) whose sperm characteristics are well described. Sperm morphology mainly differs by the presence of an acrosome in Pacific oyster which is absent in both fish species. In turbot as in Pacific oyster, a sperm maturation process along the genital tract is observed. Sperm motility is triggered by changes in osmolality for seabass and turbot and in pH for Pacific oyster. However, complementary factors are involved to maintain sperm immotile in the genital tract. Sperm movement duration is very long in Pacific oyster (2024 h), compared to turbot (35 min) and seabass (4050 s). A high capacity of ATP regeneration is observed in...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00111/22220/20059.pdf

Anesthesia in Pacific oyster, Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; De Kermoysan, Goulwen; Araya, Ricardo Gonzalez; Queau, Isabelle; Lebrun, Luc; Le Souchu, Pierrick; Mingant, Christian.
A reliable anaesthesia and sampling protocol for Pacific oysters will enable experiments to be conducted without sacrificing animals and will facilitate successive sampling of individuals for gametogenesis studies. As no such techniques were available for Crassostrea gigas, the present study aimed to define suitable anaesthetic conditions for use with this species. Three groups of ten oysters (mean weight +/- SD, 32.1 +/- 9.0 g) were anaesthetised in 5 L containers. Among different chemicals: benzocaine, eugenol and three different types of magnesium chloride (a laboratory one Flucka (R) - and two designed for agriculture - DEUSA International (R) and Dead SeaWorks (R)) and concentrations tested, one laboratory (concentration: 72 g L-1) and one...
Tipo: Text
Palavras-chave: Crassostrea gigas; Pacific oyster; Gonad sampling; Magnesium chloride; Relaxant; Anaesthesia.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6352.pdf

Marine fish culture of "new species" farmed in Europe
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Divanach, Pascal; Hussenot, Jerome; Coves, Denis; Fauvel, Christian.
In Europe, fish aquaculture in a marine environment developed following two successive waves: Atlantic salmon and then sea-bass and sea-bream. Rearing "new marine fish species" is sustained for many complementary reasons. This paper aims at presenting the aquaculture status of these "new species," research carried out and then problems and perspectives suggested by farming development in these species.
Tipo: Text
Palavras-chave: Species; Sea fishes; Fish culture; Europa; Diversification.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6558.pdf

Gamete quality in triploid Pacific oyster (Crassostrea gigas)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Malo, Florent; Quere, Claudie; Ledu, Christophe; Le Grand, Jacqueline; Benabdelmouna, Abdellah.
Triploidy induction in shellfish aims to obtain faster growth and sterility of reared individuals. Triploid Pacific oysters are most often not totally sterile, but have greatly reduced reproductive capacities compared to those recorded in diploid individuals. The description of gamete output in triploid aquatic animals is most often confined to the assessment of fecundity in females. The present work aims at further describing gamete quality of triploid Pacific oysters and comparing it to values observed in diploid individuals. Diploid and triploid oysters (produced by mating tetraploid males × diploid females) were reared in standard conditions and then transferred to the wild to allow the offspring to attain reproductive maturity. At the end of...
Tipo: Text
Palavras-chave: Crassostrea gigas; Reproduction; Triploidy; Gamete.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00277/38835/37581.pdf

Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Corporeau, Charlotte; Vanderplancke, Gwenaelle; Boulais, Myrina; Suquet, Marc; Quere, Claudie; Boudry, Pierre; Huvet, Arnaud; Madec, Stephanie.
We used a 2-DE proteomic approach to identify abundant proteins linked to oocyte quality in the Pacific oyster Crassostrea gigas, an economically important bivalve. Oocyte quality of 14 females was estimated by recording fertilisation and early developmental success until D-larval stage under controlled conditions. Proteins that were differentially expressed between females showing high or low oocyte quality were identified by nano-liquid chromatography tandem mass spectrometry. Twelve up-accumulated spots associated with low quality oocytes revealed 10 distinct proteins, including vitellogenin — breakdown products and metabolic enzymes. Eight up-accumulated spots from high quality oocytes revealed 6 distinct proteins, including chaperone molecules and...
Tipo: Text
Palavras-chave: Marine bivalves; Crassostrea gigas; Oocyte quality; Proteomics.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00098/20925/18534.pdf

The quality of great scallop (Pecten maximus) sperm after thawing
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Gourtay, Clemence; Donval, Anne; Le Goic, Nelly; Quere, Claudie; Malo, Florent; Le Grand, Jacqueline; Ratiskol, Dominique; Mingant, Christian; Fauvel, Christian.
Most publications devoted to the cryopreservation of mollusc sperm have focused on the definition of technical protocols, avoiding the description of sperm quality after thawing. The present study investigated the effects of cryopreservation on sperm quality in the great scallop. Wild scallop were fished during the natural spawning period and conditioned in the hatchery before use. Sperm samples were obtained after intragonadal injection of serotonin and cryopreserved using a previously published protocol.  Sperm quality was assessed using a panel of four parameters: sperm motility characteristics, using a a computer assisted sperm analysis plugin with Image J, intracellular ATP content using an ATP-Lite kit, sperm integrity, using flow cytometry and sperm...
Tipo: Text
Palavras-chave: Pecten maximus; Reproduction; Gamete; Sperm; Cryopreservation.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00317/42862/43912.pdf

ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster ( Crassostrea gigas )
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boulais, Myrina; Soudant, Philippe; Le Goic, Nelly; Quere, Claudie; Boudry, Pierre; Suquet, Marc.
Fertilization of the oocyte is a critical process of sexual reproduction depending among other factors on spermatozoa functionality. Mitochondria participate in many crucial processes for spermatozoa motility and fertilizing ability. The Pacific oyster, Crassostrea gigas, is characterized by a high inter-individual variability of its reproductive success which has been shown to be partially due to the variability of gamete quality. The present study explored spermatozoa characteristics such as: i) relationships among mitochondrial functionality (ATP content, mitochondrial membrane potential (MMP), reactive oxygen species production); sperm viability (percentages of live, dying, and dead); and motility; and ii) their involvement in inter-oyster variability...
Tipo: Text
Palavras-chave: Spermatozoa; Quality; Motility; Viability; ATP content; ROS production.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00386/49769/50309.pdf

Cellular responses of Pacific oyster ( Crassostrea gigas ) gametes exposed in vitro to polystyrene nanoparticles
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Gonzalez-fernandez, Carmen; Tallec, Kevin; Le Goic, Nelly; Lambert, Christophe; Soudant, Philippe; Huvet, Arnaud; Suquet, Marc; Berchel, Mathieu; Paul-pont, Ika.
While the detection and quantification of nano-sized plastic in the environment remains a challenge, the growing number of polymer applications mean that we can expect an increase in the release of nanoplastics into the environment by indirect outputs. Today, very little is known about the impact of nano-sized plastics on marine organisms. Thus, the objective of this study was to investigate the toxicity of polystyrene nanoplastics (NPs) on oyster (Crassostrea gigas) gametes. Spermatozoa and oocytes were exposed to four NPs concentrations ranging from 0.1 to 100 mg L−1 for 1, 3 and 5 h. NPs coated with carboxylic (PS-COOH) and amine groups (PS-NH2) were used to determine how surface properties influence the effects of nanoplastics. Results demonstrated the...
Tipo: Text
Palavras-chave: Nanoplastics; Oysters; Gametes; Cellular responses.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00443/55459/56976.pdf

The dinoflagellate Alexandrium minutum affects development of the oyster Crassostrea gigas, through parental or direct exposure
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Castrec, Justine; Hegaret, Helene; Alunno-bruscia, Marianne; Picard, Mailys; Soudant, Philippe; Petton, Bruno; Boulais, Myrina; Suquet, Marc; Queau, Isabelle; Ratiskol, Dominique; Foulon, Valentin; Le Goic, Nelly; Fabioux, Caroline.
Harmful algal blooms are a threat to aquatic organisms and coastal ecosystems. Among harmful species, the widespread distributed genus Alexandrium is of global importance. This genus is well-known for the synthesis of paralytic shellfish toxins which are toxic for humans through the consumption of contaminated shellfish. While the effects of Alexandrium species upon the physiology of bivalves are now well documented, consequences on reproduction remain poorly studied. In France, Alexandrium minutum blooms have been recurrent for the last decades, generally appearing during the reproduction season of most bivalves including the oyster Crassostrea gigas. These blooms could not only affect gametogenesis but also spawning, larval development or juvenile...
Tipo: Text
Palavras-chave: Harmful algal bloom (HAB); Paralytic shellfish toxin (PST); Crassostrea gigas; Gametes; Larvae.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00469/58099/60512.pdf

Impact of the toxic dinoflagellate Alexandrium catenella on Pacific oyster reproductive output: application of flow cytometry assays on spermatozoa
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Le Goic, Nelly; Hegaret, Helene; Fabioux, Caroline; Miner, Philippe; Suquet, Marc; Lambert, Christophe; Soudant, Philippe.
The toxic dinoflagellate Alexandrium catenella recurrently blooms on the coasts of France and produces Paralytic Shellfish Toxins (PSTs) that accumulate in bivalves. These toxins can affect various physiological functions including reproduction. The present study aims to validate measurements of sperm viability, DNA content and mitochondrial membrane potential in Pacific oyster Crassostrea gigas using flow cytometry coupled with fluorescent markers, and to use these measurements to assess the cellular parameters of sperm from Pacific oysters exposed to A. catenella. These parameters may influence fertilization, embryogenesis and larval development in free-spawning shellfish. Sperm viability and DNA content estimation were assessed using SYBR-14, which only...
Tipo: Text
Palavras-chave: Harmful algal bloom; Sperm quality; Cellular parameters; Flow cytometry; Pacific oyster; Crassostrea gigas; Alexandrium catenella.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00157/26853/24982.pdf

Biological characteristics of European hake (Merluccius merluccius) sperm
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Groison, Anne-laure; Suquet, Marc; Cosson, Jacky; Le Coz, Jean-rene; Jolivet, Aurelie; Garren, Francois.
Very little is known on European hake's reproductive biology and especially on biological characteristics of its sperm despite a growing interest in its aquaculture potential. This study reports, for the first time some hake sperm characteristics. After activation, the swimming phase lasts 3 min (8 min when activated with 50% sea water (SW) but lower initial velocity). The initial flagellar beat frequency (BF), velocity and percentage of motile cells decreased after 100 s. After 2 days at 4 degrees C, the mean Adenylate Energy Charge (AEC) level was 0.71. Sperm stored at 4 degrees C still showed motility after 9 days for 2 individuals. When cryopreserved, the motility recovery index of thawed spermatozoa ranged from 11.8% to 29.6%.
Tipo: Text
Palavras-chave: AEC; Gamete quality; Sperm motility; Sperm Density; Merluccius merluccius.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-6553.pdf

Experimental ecology: A key to understanding fish biology in the wild
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Rochet, Marie-joelle; Gaignon, Jean-louis.
Because of natural complexity, field studies are often inconclusive with regards to the ultimate cause of a given change observed in wild animal populations. As a consequence, there is scope to develop an Experimental Ecology Approach (EEA). In this paper, we favour the use of experimental ecology studies to evaluate the effects of environmental factors on fish biology. We identify the advantages of EEA: disentangling the effects of several factors and identifying their respective roles, generating artificial scenarios and increasing our capability to collect and analyse data. This study emphasises the constraints of this approach: fish biological performances may be altered by rearing conditions, by domestication and by the prevention of some behaviours....
Tipo: Text
Palavras-chave: Cod; Field study; Environmental parameters; Laboratory experiments; Experimental ecology.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-714.pdf

First study in cryopreserved Crassostrea angulata sperm
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Riesco, Marta F.; Felix, Francisca; Matias, Domitilia; Joaquim, Sandra; Suquet, Marc; Cabrita, Elsa.
Sperm cryopreservation is a widely employed technique that promotes alternative techniques to contribute to broodstock management or restoration programs for species of commercial interest, endangered species or species with an interesting genotype. The preservation of genetic material from improved stocks or from the original population is extremely important for the oyster aquaculture industry to prevent the potential impacts of epidemic diseases and natural disasters. The Portuguese oyster, Crassostrea angulata, was the most important species commercialized by the shellfish industry. However, inadequate management of this industry and pathology occurrences resulted in a significant decrease in natural populations. For this reason, in this work a...
Tipo: Text
Palavras-chave: Crassostrea angulata; Sperm; Cryopreservation; Motility; Viability; Fertilization.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00333/44432/44098.pdf

Differential protein expression during sperm maturation and capacitation in an hermaphroditic bivalve,     Pecten maximus     (Linnaeus, 1758)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boonmee, A.; Berthelin, C. Heude; Kingtong, S.; Pauletto, M.; Bernay, B.; Adeline, B.; Suquet, Marc; Sourdaine, P.; Kellner, K.
In order to investigate the mechanisms of final maturation and capacitation of spermatozoa in Pecten maximus, we used a 2D proteomic approach coupled with MALDI-TOF/TOF mass spectrometry (MS) and bioinformatics search against the Pecten database, to set up a reference map of the proteome of spawned spermatozoa, and identified 133 proteins on the basis of the EST database. These proteins are mainly involved in energy production, ion and electron transport (44%), cell movement (22%) and developmental processes (10%). Comparison between proteomes of spermatozoa collected before and after transit through the genital ducts of P. maximus led to the identification of differentially expressed proteins. Most of them are associated with energy metabolism (aconitate...
Tipo: Text
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00353/46386/47178.pdf

Effects of Alexandrium minutum exposure on nutrition-related processes and reproductive output in oysters Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Haberkorn, Hansy; Lambert, Christophe; Le Goic, Nelly; Moal, Jeanne; Suquet, Marc; Gueguen, Marielle; Sunila, Inke; Soudant, Philippe.
This study assessed the effects of an artificial bloom of the toxin-producing dinoflagellate, Alexandrium minutum, upon nutrition related processes and reproductive output of the Pacific oyster, Crassostrea gigas. Oysters were exposed to A. minutum, Paralytic Shellfish Toxins (PST) producer and compared to a control batch of oysters fed Isochrysis galbana clone Tahitian (T.Iso). The experiment was performed in June 2008, when oysters were found ripe. Several physiological variables of oysters, such as PSTs accumulation, digestive gland and histological observations as well as spermatozoa quality, were measured at the end of the exposure. Results indicate that the digestive gland was greatly impacted upon A. minutum exposure. Monoacylglycerol and...
Tipo: Text
Palavras-chave: Harmful algae; Alexandrium minutum; Oyster; Nutrition-related processes; Reproductive output.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00008/11927/8653.pdf

Comparative analysis of male germ cell proliferation and apoptosis in wild and captive Atlantic bluefin tuna (Thunnus thynnus L.)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Zupa, R.; Fauvel, Christian; Mylonas, C. C.; Santamaria, N.; Valentini, L.; Pousis, C.; Papadaki, M.; Suquet, Marc; De La Gandara, F.; Bello, G.; Metrio, G.; Corriero, A.
The most commonly observed reproductive dysfunction in male fishes reared in captivity is reduction in sperm volume and quality. The Atlantic bluefin tuna Thunnus thynnus (Osteichthyes: Scombridae) is one of the few large pelagic and migratory marine fishes maintained in captivity with the purpose of establishing breeding populations to support an aquaculture industry. The objectives of the present study were to compare male germ cell proliferation and apoptosis between wild and captive individuals at two different phases of the spermatogenetic cycle, and to evaluate sperm motility characteristics of captive individuals. Histological observations were performed to analyze testicular activity, and germ cell proliferation and apoptosis were evaluated through...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00125/23579/25462.pdf

La domestication des Gadidés : le cas de la morue et du lieu jaune
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Fauvel, Christian; Gaignon, Jean-louis.
The term "domestication" has a legislative meaning when it applies to species submitted to selection pressures, but also a literary one, according to the time since which the species live closed to men and the possibility of controlling their reproduction. In terms of legislation, none of both species of gadids object of this article, cod and pollack, can be considered as domestic. From a literary point of view, 140 years of research and the control of the reproduction, give to the cod a domestic status. It is not the case with pollack, only studied since ten years and the reproduction of which is still badly controlled. The weak differences observed between the performances of breeding and wild cods focuse the still limited impact of the domestication on...
Tipo: Text
Ano: 2004
URL: http://archimer.ifremer.fr/doc/00272/38282/36471.pdf

Reply to Lenz et al.: Quantifying the smallest microplastics is the challenge for a comprehensive view of their environmental impacts
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Huvet, Arnaud; Paul-pont, Ika; Fabioux, Caroline; Lambert, Christophe; Suquet, Marc; Thomas, Yoann; Robbens, Johan; Soudant, Philippe; Sussarellu, Rossana.
Tipo: Text
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00345/45604/45224.pdf

Quality assessment of cryopreserved black-lip pearl oyster Pinctada margaritifera spermatozoa
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Demoy-schneider, Marina; Schmitt, Nelly; Le Pennec, Gael; Suquet, Marc; Cosson, Jacky.
High quality of sperm is essential to a high fertilization rate, especially post- cryopreservation. Assessment of sperm integrity, motility and energy reserves before cryopreservation is necessary for selection of milt with optimal fertilizing potential. We describe the effect of cryopreservation on the quality of black-lip pearl oyster, Pinctada margaritifera var. cumingii sperm. Evaluated quality indices of fresh and frozen/thawed P. margaritifera spermatozoa, included morphology, ultrastructure and motility characteristics relative to the energy content (ATP) and its capacity to be sustained by mitochondrial respiration. Morphology and ultrastructure were quantitatively evaluated using images obtained by optical microscopy assisted by the Image J...
Tipo: Text
Palavras-chave: Spermatozoa quality; Cryopreservation; Pinctada margaritifera; Motility; Metabolism; CASA.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00453/56421/58114.pdf

Some characteristics of sperm motility in European hake (Merluccius merluccius, L., 1758)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Groison, A. -l.; Fauvel, Christian; Suquet, Marc; Kjesbu, O. S.; Le Coz, Jean-rene; Mayer, I.; Cosson, J.
P&gt;The objective of this paper is to characterize some of the sperm motility parameters in European hake (Merluccius merluccius), which is considered to be a species with aquaculture potential. The total ATP, ADP and AMP concentrations were determined using high-performance liquid chromatography on hake sperm samples collected during the winter-early spring in the Bay of Biscay (France) (n = 22) and on hake sperm samples collected during the summer-early autumn in waters off Western Norway (n = 5). The Adenylate Energy Charge (AEC) was deduced from these data. Computer Assisted Sperm Analysis (CASA) was used to measure a series of parameters characterizing the motility and the sperm swimming performances. Changes in salinity of the swimming medium...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00015/12644/9883.pdf

Selection method of new candidates for finfish aquaculture: the case of the French Atlantic, the Channel and the North Sea coasts
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Quemener, Loic; Suquet, Marc; Mero, David; Gaignon, Jean-louis.
At present, European marine fish farming is based on sea bream and sea bass. A trend for diversification is sustained by the diversity of environmental conditions, by the availability of new production techniques such as recirculating systems, by an increase in rearing yields, by new market trends and by the possibilities to reduce risks of disease outbreak. Already reared fish species were chosen considering a limited number of criteria such as a high selling price and the availability of juveniles or breeders in the wild. This paper proposes a new selection method of fish species as candidates for aquaculture development on the French Atlantic, the Channel and the North Sea coasts. Using a three-phase procedure, candidates were selected among 20 000 fish...
Tipo: Text
Palavras-chave: Gadiforms; Cod; Diversification; Species selection; New species.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-567.pdf

Oyster reproduction is affected by exposure to polystyrene microplastics
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Sussarellu, Rossana; Suquet, Marc; Thomas, Yoann; Lambert, Christophe; Fabioux, Caroline; Pernet, Marie Eve Julie; Le Goic, Nelly; Quillien, Virgile; Mingant, Christian; Epelboin, Yanouk; Corporeau, Charlotte; Guyomarch, Julien; Robbens, Johan; Paul-pont, Ika; Soudant, Philippe; Huvet, Arnaud.
Plastics are persistent synthetic polymers that accumulate as waste in the marine environment. Microplastic (MP) particles are derived from the breakdown of larger debris or can enter the environment as microscopic fragments. Because filter-feeder organisms ingest MP while feeding, they are likely to be impacted by MP pollution. To assess the impact of polystyrene microspheres (micro-PS) on the physiology of the Pacific oyster, adult oysters were experimentally exposed to virgin micro-PS (2 and 6 µm in diameter; 0.023 mg·L−1) for 2 mo during a reproductive cycle. Effects were investigated on ecophysiological parameters; cellular, transcriptomic, and proteomic responses; fecundity; and offspring development. Oysters preferentially ingested the 6-µm micro-PS...
Tipo: Text
Palavras-chave: Microplastic; Reproduction; Energy allocation; Oyster.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00311/42233/41600.pdf

Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boulais, Myrina; Corporeau, Charlotte; Huvet, Arnaud; Bernard, Ismael; Quere, Claudie; Quillien, Virgile; Fabioux, Caroline; Suquet, Marc.
Pacific oyster hatchery production remains highly variable, partially due to inconsistent gamete quality. However, little attention has been paid to oocyte quality in Pacific oyster compared to important vertebrate aquaculture species such as fishes. The aims of the present study were to identify criteria of oocyte and trochophore quality used as predictive tools for embryo developmental success at the D-larval stage. Twenty-six potential proxies of oocyte and trochophore quality were studied on 25 mature females, as determined using histology. Some morphological and biochemical characteristics of mature oocytes were described. Protein appeared to be the main constituent of fully mature Pacific oyster oocytes. Neutral lipids accounted for 61% of the total...
Tipo: Text
Palavras-chave: Crassostrea gigas; Oocyte quality; Trochophore; Lipid; Gene expression; ATP.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00239/35010/33544.pdf

Survival, Growth and Reproduction of Cryopreserved Larvae from a Marine Invertebrate, the Pacific Oyster (Crassostrea gigas)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Labbe, Catherine; Puyo, Sophie; Mingant, Christian; Quittet, Benjamin; Boulais, Myrina; Queau, Isabelle; Ratiskol, Dominique; Diss, Blandine; Haffray, Pierrick.
This study is the first demonstration of successful post-thawing development to reproduction stage of diploid cryopreserved larvae in an aquatic invertebrate. Survival, growth and reproductive performances were studied in juvenile and adult Pacific oysters grown from cryopreserved embryos. Cryopreservation was performed at three early stages: trochophore (13 +/- 2 hours post fertilization: hpf), early D-larvae (24 +/- 2 hpf) and late D-larvae (43 +/- 2 hpf). From the beginning (88 days) at the end of the ongrowing phase (195 days), no mortality was recorded and mean body weights did not differ between the thawed oysters and the control. At the end of the growing-out phase (982 days), survival of the oysters cryopreserved at 13 +/- 2 hpf and at 43 +/- 2 hpf...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00190/30092/28635.pdf

Changes in motility, ATP content, morphology and fertilisation capacity during the movement phase of tetraploid Pacific oyster (Crassostrea gigas) sperm
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Labbe, C.; Brizard, Raphael; Donval, Anne; Le Coz, Jean-rene; Quere, Claudie; Haffray, P.
Changes in sperm features during the movement phase are especially interesting to study in external fertilization species whose sperm duration movement is long because this implies a significant adaptation of moving cells to the external medium. This study describes the changes in tetraploid Pacific oyster sperm characteristics in relation to time post activation. Sperm individually collected on three tetraploid males were activated in seawater. Their features were analysed over a 24 h period and compared to a sperm pool collected on three diploid males as a reference. The percentage of motile spermatozoa, the intracellular ATP content, and the fine structure of spermatozoa were studied in relation to time post activation. Furthermore, the fertilisation...
Tipo: Text
Palavras-chave: Crassostrea gigas; Sperm; Tetraploid; Motility; ATP; Ultrastructure.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00006/11756/8639.pdf

Analysis of turbot (Scophthalmus maximus) broodstock pilot scale production.
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Omnes, Marie-helene; Normant, Yvon; Suquet, Marc; Fauvel, Christian.
Rearing turbot (Scophthalmus maximus) reaches the commercial scale. With the hatchery output increasing, more attention needs to be focussed on broodstocks performance both in terms of quantity and viability of eggs. The techniques of shifting the environmental conditions allow a production of eggs all year round. However, egg quality tends to be highly variable. This paper examines over a two-year period, the improvement in egg quality and its consequences on the production of larvae by a strict management of spawners.
Tipo: Text
Ano: 1991
URL: http://archimer.ifremer.fr/doc/00169/28061/29430.pdf

Improvement of chilled seabass sperm conservation using a cell culture medium
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Fauvel, Christian; Boryshpolets, Sergey; Cosson, J.; Leedy, J. G. Wilson; Labbe, C.; Haffray, Pierrick; Suquet, Marc.
The sperm of seabass is very fragile and it quickly loses its ability to fertilize after collection either if kept undiluted or in classic saline media. In order to avoid cryopreservation when only short conservation is required, the process of sperm management including sperm collection, sperm dilution rate in storage medium and storage medium composition, was subject to experimental trials. A concentration of 20% urine generated a low pH of seminal fluid, and it immediately altered the motility ability. However, pH did not seem to be the key agent of motility prevention since sperm dilution in Leibovitz culture medium (L15) or in classic saline medium both presenting a similar low pH (7.3) did not affect motility. L15 increased the duration of sperm...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00111/22221/20066.pdf

NMR relaxometry as a potential non-invasive routine sensor for characterization of phenotype in Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Davenel, Armel; Pouvreau, Stephane; Cambert, Mireille; Suquet, Marc; Mariette, Francois.
MR imaging is the most appropriate non-invasive technique for quantifying the growth of somatic and gonad tissues and to determine sex in the Pacific oyster, Crassostrea gigas. However, this technique is too costly for field studies where oysters are used as bioindicators of environmental quality or to be applied routinely in hatcheries. We have tested the ability of low Nuclear Magnetic Resonance relaxometry, a much less expensive technique, to obtain phenotype parameters that can be used to monitor the physiological state of oysters. NMR measurements were carried out at three different periods using a low field spectrometer equipped with a 50 mm diameter probe to investigate 60 oysters in their first year of maturity, which were then dissected to measure...
Tipo: Text
Palavras-chave: Sex identification; Growth; NMR; Crassostrea gigas; Phenotype characterization.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6456.pdf

Procédures d'échantillonnage pour la conservation des espèces aquacoles dans la Cryobanque Nationale
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Vandeputte, Marc; Labbe, Catherine; Suquet, Marc; Fauvel, Christian; Goardon, Lionel; Duclos, Delphine; Danchin-burge, Coralie; Vergnet, Alain; Rault, Paul; Degremont, Lionel; Benabdelmouna, Abdellah; Brizard, Raphael; Haffray, Pierrick.
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00098/20975/18597.pdf

Setting of a procedure for experimental incubation of Pacific oyster (Crassostrea gigas) embryos
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Amourda, Christopher; Mingant, Christian; Queau, Isabelle; Lebrun, Luc; Brizard, Raphael.
Standardized experimental protocols designed to study Pacific oyster (Crassostrea gigas) gamete quality have not been previously published. Gamete sampling variations and confounding effect of interacting factors result in large variations between replicates, decreasing the effects of the studied factors. This work aims at defining a standardized procedure for incubation of oyster embryos designed for experimental purposes.   In a first phase, four experiments were developed to improve embryo sampling and handling. They showed that a minimum of 50 embryos must be counted to decrease the variation between counting replicates. For reliable results, sampling must be carried out from 0 to 7 min after a careful agitation of seawater containing embryos...
Tipo: Text
Palavras-chave: Crassostrea gigas; Pacific oyster; Experimental; Embryo; Incubation.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3593.pdf