Consulta: creatorFacets:"TAHA,R.M."
Registros recuperados: 3
Data/hora: 04/06/2024 03:30:49

The Role of Plant Growth Regulators in the Development of in vitro Flowering, Histology and Ultrastructural Studies in Impatiens balsamina cv. Dwarf Bush
Provedor de dados: Planta Daninha
Autores: MOHAMED,N.; TAHA,R.M.; RAZAK,U.N.A.A.; ELIAS,H.
ABSTRACT: An efficient protocol for in vitro flowering was successfully established for Impatiens balsamina cv Dwarf Bush, an important medicinal plant, through tissue culture techniques. Shoot, stem and petiole explants obtained from 4 week-old aseptic seedlings cultured on MS medium supplemented with different concentrations of plant growth regulator (PGR) were used for in vitro flower induction. Gibberellic acid (GA3), benzylaminopurine (BAP) and kinetin (Kin) treatment singly applied in MS media (pH 5.8), could all stimulate flowering at 23-26 oC with photoperiod of 16 hours light and 8 hours dark. It was observed that shoot explants were more responsive than stem explants in floral formation. Regeneration was achieved via direct organogenesis. For...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro regeneration; Ultrastructural; Histology; Gibberellic acid; Flowering response.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100211

Micropropagation and Cellular Behaviour Changes During in vitro Flowering of Impatiens balsamina
Provedor de dados: Planta Daninha
Autores: MOHAMED,N.; TAHA,R.M.; RAZAK,U.N.A.A.
ABSTRACT: In this study, the micropropagation of Impatiens balsamina was established from stem and shoot explants. The effects of GA3 and glutathione on the morphogenesis of this species were also investigated, in order to induce in vitro flowering. It was found that the optimum in vitro plant regeneration was achieved on MS medium supplemented with 1.0 mg L-1 GA3 and in vitro flowering was also obtained from the same medium after 4 weeks of culture. To understand cellular behavior during in vitro flowering, Mitotic Index (MI), chromosome counts, measurement of mean cell and nuclear areas, DNA measurements and ploidy levels were analyzed from in vivo plants, in vitro grown plants and plantlets that flowered in vitro. The chromosome count was the same for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Regeneration; Gibberellic acid; Glutathione; Flowering response; Cellular behavior and acclimatization.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100236

Detection of Somaclonal Variation in Micropropagated and Acclimatized Plantlets of Oryza sativa MRQ 74 from Stem Explants
Provedor de dados: Planta Daninha
Autores: SALEH,A.; TAHA,R.M.; MAHMAD,N.; ELIAS,H.; HAMID,H.A.A.
ABSTRACT: In plant tissue culture system, the excessive supply of plant growth hormones may inhibit further growth and development of the regenerants and sometimes it may induce somaclonal variation. In this study, the optimum concentration of plant growth hormones for in vitro regeneration of Oryza sativa L. cv. MRQ 74 was identified and subsequently the occurrence of somaclonal variation of regenerated plantlets was determined through cytological analysis of in vivo and in vitro grown plantlets. MS media supplemented with 0.1 mg L-1 BAP in combination with 0.1 mg L-1 NAA was found to be the optimum concentration for shoots and roots induction of Oryza sativa L. cv. MRQ 74. Plantlets derived from MS media supplemented with 0.5 mg L-1 2,4-D showed higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant growth hormones; Oryza sativa L. cv. MRQ 74; Survival rate; Agronomic parameters; Mitotic index; Ploidy level.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100212