Consulta: creatorFacets:"TASAT,DEBORAH R."
Registros recuperados: 1
Data/hora: 16/06/2024 09:18:15

Active caspase-3 expression levels as bioindicator of individual radiosensitivity
Provedor de dados: 42
Autores: SANTOS,NEYLIANE F.G. DOS; SILVA,RAFAEL F.; PINTO,MARCELA M.P.L.; SILVA,EDVANE B. DA; TASAT,DEBORAH R.; AMARAL,ADEMIR.
ABSTRACT Several molecules and events involved in cell response to radiation-induced damage have been investigated towards a personalized radiotherapy. Considering the importance of active caspase-3 in the proteolytic cascade that ensures radiation-induced apoptosis execution, this research was designed to evaluate the expression levels of this protein as a bioindicator of individual radiosensitivity. Peripheral blood samples of 10 healthy individuals were gamma-irradiated (cobalt-60 source) with 1, 2 and 4 Gy (control: non-irradiated samples), and active caspase-3 expression levels were measured in lymphocytes, by flow cytometry, ex vivo and after different times of in vitro incubation (24, 48 and 72 hours). Short-term incubation of 24 h was the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Active caspase-3; Apoptosis; Flow cytometry; Ionizing radiation; Lymphocytes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200649