Consulta: creatorFacets:"Taketomi,E.A."
Registros recuperados: 3
Data/hora: 13/06/2024 01:10:18

Humoral and cellular immune responses to Blomia tropicalis and concanavalin A-binding fractions in atopic patients
Provedor de dados: 56
Autores: Alves,R.; Silva,D.A.O.; Fernandes,J.F.C.; Almeida,K.C.; Ynoue,L.H.; Bernardes,C.T.V.; Moreira,P.F.S.; Gennari-Cardoso,M.L.; Sung,S.J.; Taketomi,E.A.
Blomia tropicalis, Dermatophagoides pteronyssinus and D. farinae are prevalent house dust mites. Concanavalin A-binding components derived from B. tropicalis (Bt-ConA extract) are highly immunogenic in allergic diseases. The aim of the present study was to evaluate the humoral and cellular immune responses to B. tropicalis in mite-sensitized patients. A total of 137 patients with allergic rhinitis with/without asthma and 109 non-atopic subjects were selected and analyzed by the skin prick test, and for total serum IgE and specific IgE levels to both Bt-total and Bt-ConA extracts, their proliferative response and cytokine (IFN-γ and IL-5) production by peripheral blood mononuclear cells (PBMC) stimulated with both extracts. Skin prick test showed that 70%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blomia tropicalis; Concanavalin A; Cellular proliferation; IFN-γ; IL-5; Immunoglobulin E.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900006

IgE cross-reactivity between Lolium multiflorum and commercial grass pollen allergen extracts in Brazilian patients with pollinosis
Provedor de dados: 56
Autores: Bernardes,C.T.; Moreira,P.F.; Sopelete,M.C.; Vieira,F.A.; Sung,S.S.; Silva,D.A.; Taketomi,E.A.
Lolium multiflorum (Lm) grass pollen is the major cause of pollinosis in Southern Brazil. The objectives of this study were to investigate immunodominant components of Lm pollen allergens and the cross-reactivity of IgE with commercial grass pollen allergen extracts. Thirty-eight serum samples from patients with seasonal allergic rhinitis (SAR), 35 serum samples from patients with perennial allergic rhinitis (PAR) and 30 serum samples from non-atopic subjects were analyzed. Allergen sensitization was evaluated using skin prick test and serum IgE levels against Lm pollen extract were determined by ELISA. Inhibition ELISA and immunoblot were used to evaluate the cross-reactivity of IgE between allergens from Lm and commercial grass pollen extracts, including...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pollinosis; Allergic rhinoconjunctivitis; Grass pollen allergen; Lolium multiflorum; Cross-reactivity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200007

Responses of IgE, IgG1, and IgG4 to concanavalin A-binding Blomia tropicalis antigens in allergic patients
Provedor de dados: 56
Autores: Almeida,K.C.; Silva,D.A.O.; Gennari-Cardoso,M.L.; Cunha-Júnior,J.P.; Alves,R.; Ynoue,L.H.; Resende,R.O.; Sung,S.J.; Taketomi,E.A.
Blomia tropicalis (Bt) and Dermatophagoides pteronyssinus (Dp) are the prevalent house dust mites in tropical countries and are associated with allergic diseases. Glycosylated antigens are highly immunogenic and involved in different pathologies. We evaluated the presence of IgE, IgG1, and IgG4 to concanavalin A-binding antigens (Bt-Con-A) isolated from Bt-total extract in sera of allergic and non-allergic subjects. Bt-total and Bt-Con-A extracts were evaluated by SDS-PAGE and ELISA for reacting with IgE, IgG1, and IgG4 in sera of 121 patients with allergic rhinitis and 36 non-allergic individuals. All subjects were skin prick tested with Bt-total extract and inhibition tests were performed for IgE, IgG1, and IgG4 using both extracts (Bt-total and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allergy; Blomia tropicalis; Concanavalin A; Immunoglobulin E; Immunoglobulin G1; Immunoglobulin G4.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100008