Consulta: creatorFacets:"Tamashiro,W.M.S.C."
Registros recuperados: 4
Data/hora: 10/06/2024 10:15:10

Aging reduces the primary humoral response and the in vitro cytokine production in mice
Provedor de dados: 56
Autores: Simioni,P.U.; Costa,E.H.; Tamashiro,W.M.S.C.
Aging is accompanied by a decrease in several physiological functions that make older individuals less responsive to environmental challenges. In the present study, we analyzed the immune response of female BALB/c mice (N = 6) of different ages (from 2 to 96 weeks) and identified significant age-related alterations. Immunization with hapten-protein (trinitrophenyl-bovine serum albumin) conjugates resulted in lower antibody levels in the primary and secondary responses of old mice (72 weeks old). Moreover, young mice (2, 16, and 32 weeks old) maintained specific antibodies in their sera for longer periods after primary immunization than did old mice. However, a secondary challenge efficiently induced memory in old mice, as shown by the increased antibody...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Cytokines; Antibody; Proliferation; T cell response; T cell subsets.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800012

Effect of aging and oral tolerance on dendritic cell function
Provedor de dados: 56
Autores: Simioni,P.U.; Fernandes,L.G.R.; Gabriel,D.L.; Tamashiro,W.M.S.C.
Oral tolerance can be induced in some mouse strains by gavage or spontaneous ingestion of dietary antigens. In the present study, we determined the influence of aging and oral tolerance on the secretion of co-stimulatory molecules by dendritic cells (DC), and on the ability of DC to induce proliferation and cytokine secretion by naive T cells from BALB/c and OVA transgenic (DO11.10) mice. We observed that oral tolerance could be induced in BALB/c mice (N = 5 in each group) of all ages (8, 20, 40, 60, and 80 weeks old), although a decline in specific antibody levels was observed in the sera of both tolerized and immunized mice with advancing age (40 to 80 weeks old). DC obtained from young, adult and middle-aged (8, 20, and 40 weeks old) tolerized mice were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Oral tolerance; Dendritic cell; Antibody; Proliferation; Cytokine secretion.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100010

Role of Tamm-Horsfall protein in the binding and in vivo phagocytosis of type 1 fimbriated Escherichia coli by mouse peritoneal macrophages
Provedor de dados: 56
Autores: Bastos,A.C.S.C.; Santos,L.B.; Tamashiro,W.M.S.C.; Yamada,A.T.; Oliveira,U.M.; Yano,T.
Tamm-Horsfall glycoprotein (THP) contains manno-oligosaccharides that are recognized by type 1 fimbriae (F1) of Escherichia coli. In the present study, we examined the in vivo phagocytic activity of mouse peritoneal macrophages after treatment of bacteria with THP. At low THP concentrations (12.5 µg/ml and 50 µg/ml) no significant difference was observed in the phagocytosis of E. coli F1+. However, at high THP concentrations (500 µg/ml and 1250 µg/ml) we obtained a reduction of bacterial phagocytosis by mouse peritoneal macrophages.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tamm-Horsfall protein; Type 1 fimbriated E. coli; Phagocytosis.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700011

Aging alters the production of iNOS, arginase and cytokines in murine macrophages
Provedor de dados: 56
Autores: Cecílio,C.A.; Costa,E.H.; Simioni,P.U.; Gabriel,D.L.; Tamashiro,W.M.S.C.
The limited amount of information on the primary age-related deficiencies in the innate immune system led us to study the production of inducible nitric oxide synthase (iNOS), arginase, and cytokines in macrophages of young (8 weeks old) and old (72 weeks old) female BALB/c mice. We first evaluated iNOS and arginase inducers on peritoneal (PMΦ) and bone marrow-derived (BMMΦ) macrophages of young BALB/c and C57BL/6 mice, and then investigated their effects on macrophages of old mice. Upon stimulation with lipopolysaccharide (LPS), resident and thioglycolate-elicited PMΦ from young mice presented higher iNOS activity than those from old mice (54.4%). However, LPS-stimulated BMMΦ from old mice showed the highest NO levels (50.1%). Identical NO levels were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; INOS; Arginase; Cytokines; Murine macrophages; Inflammatory stimuli.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700010