Consulta: creatorFacets:"Teibler,P"
Registros recuperados: 2
Data/hora: 02/06/2024 00:54:00

IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: cross-reaction with venoms from Bothrops species from Argentina
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Rodríguez,JP; De Marzi,MC; Maruñak,S; Teibler,P; Acosta,O; Malchiodi,EL; Leiva,LC.
We examined the ability of IgG anti-crotalic PLA2 to cross-react with Bothrops spp. venoms, from snakes found in the northeast of Argentina. Immunoblotting and ELISA tests showed that IgG anti-crotalic PLA2 recognize antigens of bothropic venoms. Indirect hemolytic activity tests showed that the quantity of antibodies that neutralized 50% of Crotalus durissus terrificus venom (ED50: 2.1 mg IgG anti-crotalic PLA2/100 µg of venom) were also able to neutralize venom from other snakes in the following proportion: 34% of B. alternatus, 18% of B. diporus and 12% of B. jararacussu. Likewise, direct PLA2 activity neutralization tests showed a similar cross-neutralization pattern including 56% of B. alternatus, 29% of B. diporus and 30% of B. jararacussu. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops; Crotalus; PLA2; Neutralization; Cross-reaction.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300008

Isolation and biological characterization of a basic phospholipase A2 from Bothrops jararacussu snake venom
Provedor de dados: Biocell
Autores: Maruñak,S.L.; Leiva,L; Garcia Denegri,M.E.; Teibler,P; Acosta De Pérez,O.
A phospholipase A2 has been isolated from Bothrops jararacussu venom from snakes that inhabit the northeast region of Argentina. The present study describes in vivo and in vitro biological activities of phospholipase A2 from B. jararacussu as well as isolation details of its. Venom was obtained by milking of adult snakes which were housing in wood reptile cages of varying dimensions in heated (20-30ºC) rooms. Snakes received a weekly diet of mice and water was available ad libitum for drinking and soaking. The enzyme was purified by gel filtration on a Sephadex G-75 column followed by ion exchange chromatography on a SP-Sephadex C25 column. The major peak belonging to proteins was retained in the cation exchanger and then eluted using a concentration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops jararacussu; Phospholipase A2; Isolation.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300001