Consulta: creatorFacets:"Thiery, Valerie"
Registros recuperados: 7
Data/hora: 08/06/2024 13:53:27

Study on the microalgal pigments extraction process: Performance of microwave assisted extraction
Provedor de dados: 5
Autores: Pasquet, Virginie; Cherouvrier, Jean-rene; Farhat, Firas; Thiery, Valerie; Piot, Jean-marie; Berard, Jean-baptiste; Kaas, Raymond; Serive, Benoit; Patrice, Thierry; Cadoret, Jean-paul; Picot, Laurent.
The performance of microwaves irradiation (MAE and VMAE) to extract pigments from two marine microalgae was compared to conventional processes (cold and hot soaking and ultrasound-assisted extraction). Pigments were quantified by RP-HPLC and extraction performance was assessed regarding rapidity, reproducibility and extraction yields. Scanning electron microscopy was used at all extraction steps to assess the impact of the process on microalgal cell integrity. Freeze-drying and pigments extraction preserved microalgae cell integrity (except sonication) and evoked agglutination in superposed cells layers. All processes performed on Dunaliella tertiolecta (chlorophyte) lead to rapid pigments extraction, and equivalent pigments extraction yields, the absence...
Tipo: Text
Palavras-chave: Pigments; Microalgae; Microwave; Extraction; Chlorophyll; Fucoxanthin; Carotene; MAE; VMAE.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00029/14059/11533.pdf

Antiproliferative Activity of Cyanophora paradoxa Pigments in Melanoma, Breast and Lung Cancer Cells
Provedor de dados: 5
Autores: Baudelet, Paul-hubert; Gagez, Anne-laure; Berard, Jean-baptiste; Juin, Camille; Bridiau, Nicolas; Kaas, Raymond; Thiery, Valerie; Cadoret, Jean-paul; Picot, Laurent.
The glaucophyte Cyanophora paradoxa (Cp) was chemically investigated to identify pigments efficiently inhibiting malignant melanoma, mammary carcinoma and lung adenocarcinoma cells growth. Cp water and ethanol extracts significantly inhibited the growth of the three cancer cell lines in vitro, at 100 mu g.mL(-1). Flash chromatography of the Cp ethanol extract, devoid of c-phycocyanin and allophycocyanin, enabled the collection of eight fractions, four of which strongly inhibited cancer cells growth at 100 mu g.mL(-1). Particularly, two fractions inhibited more than 90% of the melanoma cells growth, one inducing apoptosis in the three cancer cells lines. The detailed analysis of Cp pigment composition resulted in the discrimination of 17 molecules, ten of...
Tipo: Text
Palavras-chave: Pigments; Microalgae; Cyanophora paradoxa; Melanoma; Cancer; Apoptosis; Zeaxanthin; Cryptoxanthin.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00177/28818/27289.pdf

Antiproliferative Activity of Violaxanthin Isolated from Bioguided Fractionation of Dunaliella tertiolecta Extracts
Provedor de dados: 5
Autores: Pasquet, Virginie; Morisset, Perrine; Ihammouine, Said; Chepied, Amandine; Aumailley, Lucie; Berard, Jean-baptiste; Serive, Benoit; Kaas, Raymond; Lanneluc, Isabelle; Thiery, Valerie; Lafferriere, Mathieu; Piot, Jean-marie; Patrice, Thierry; Cadoret, Jean-paul; Picot, Laurent.
Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose- dependent growth inhibition in continuous and discontinuous treatments, at...
Tipo: Text
Palavras-chave: Pigments; Microalgae; Dunaliella; Violaxanthin; Carotenoid; Cancer; Apoptosis.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00040/15142/12575.pdf

UPLC-MSE Profiling of Phytoplankton Metabolites: Application to the Identification of Pigments and Structural Analysis of Metabolites in Porphyridium purpureum
Provedor de dados: 5
Autores: Juin, Camille; Bonnet, Antoine; Nicolau, Elodie; Berard, Jean-baptiste; Devillers, Romain; Thiery, Valerie; Cadoret, Jean-paul; Picot, Laurent.
A fast and high-resolution UPLC-MSE analysis was used to identify phytoplankton pigments in an ethanol extract of Porphyridium purpureum (Pp) devoid of phycobiliproteins. In a first step, 22 standard pigments were analyzed by UPLC-MSE to build a database including retention time and accurate masses of parent and fragment ions. Using this database, seven pigments or derivatives previously reported in Pp were unequivocally identified: beta,beta-carotene, chlorophyll a, zeaxanthin, chlorophyllide a, pheophorbide a, pheophytin a, and cryptoxanthin. Minor amounts of Divinyl chlorophyll a, a chemotaxonomic pigment marker for prochlorophytes, were also unequivocally identified using the database. Additional analysis of ionization and fragmentation patterns...
Tipo: Text
Palavras-chave: Carotenoid; Chlorophyll; Dereplication; Divinyl chlorophyll a; Galactosyldiacylglycerol; Gracilamide; Mass spectrometry; MSE; Phytoplankton; Pigment; Porphyridium purpureum; UPLC.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00269/37994/36069.pdf

Antimelanoma activity of Heterocapsa triquetra pigments
Provedor de dados: 5
Autores: Haguet, Quentin; Bonnet, Antoine; Berard, Jean-baptiste; Goldberg, Joelle; Joguet, Nicolas; Fleury, Audrey; Thiery, Valerie; Picot, Laurent.
Heterocapsa triquetra (Ht), a non-toxic dinophyte, was selected to investigate the presence of pigments inhibiting melanoma cells growth. We developed an innovative microwave-assisted extraction process (MAE), determined the Ht pigment composition using Reverse Phase-High Performance Liquid Chromatography (HPLC) and high-resolution Ultra Performance Liquid Chromatography-Mass Spectrometry E (UPLC-MSE) analysis and assessed their antiproliferative activity in human invasive melanoma cells (A2058) grown in vitro. MAE allowed a fast and efficient extraction of Ht pigments as compared with conventional processes (soaking, sonication). Seventeen pigments and derivatives were unequivocally identified by UPLC-MSᴱ in the Ht extract, among which two were supposed...
Tipo: Text
Palavras-chave: Dinophyte; Heterocapsa; Melanoma; Microalgae; Microwave assisted extraction; Pigment.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00390/50127/50806.pdf

Microwave-assisted extraction of phycobiliproteins from Porphyridium purpureum
Provedor de dados: 5
Autores: Juin, Camille; Cherouvrier, Jean-rene; Thiery, Valerie; Gagez, Anne-laure; Berard, Jean-baptiste; Joguet, Nicolas; Kaas, Raymond; Cadoret, Jean-paul; Picot, Laurent.
In the present study, microwave-assisted extraction was first employed to extract the phycobiliproteins of Porphyridium purpureum (Pp). Freeze-dried Pp cells were subjected to microwave-assisted extraction (MAE) to extract phycoerythin (PE), phycocyanin (PC), and allophycocyanin (APC). MAE combined reproducibility and high extraction yields and allowed a 180- to 1,080-fold reduction of the extraction time compared to a conventional soaking process. The maximal PE extraction yield was obtained after 10-s MAE at 40 A degrees C, and PE was thermally damaged at temperatures higher than 40 A degrees C. In contrast, a flash irradiation for 10 s at 100 A degrees C was the best process to efficiently extract PC and APC, as it combined a high temperature necessary...
Tipo: Text
Palavras-chave: Allophycocyanin; MAE; Microalgae; Microwave; Phycobiliprotein; Phycocyanin; Phycoerythrin; Porphyridium; Thylakoid.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00253/36439/35169.pdf

Zeaxanthin from Porphyridium purpureum induces apoptosis in human melanoma cells expressing the oncogenic BRAF V600E mutation and sensitizes them to the BRAF inhibitor vemurafenib
Provedor de dados: 5
Autores: Juin, Camille; De Oliveira Junior, Raimundo Gonsalves; Fleury, Audrey; Oudinet, Chloe; Pytowski, Lior; Berard, Jean-baptiste; Nicolau, Elodie; Thiery, Valerie; Lanneluc, Isabelle; Beaugeard, Laureen; Prunier, Gregoire; Guedes Da Silva Almeida, Jackson Roberto; Picot, Laurent.
Zeaxanthin, an abundant carotenoid present in fruits, vegetables and algae was reported to exert antiproliferative activity and induce apoptosis in human uveal melanoma cells. It also inhibited uveal melanoma tumor growth and cell migration in nude mice xenograft models. Here we report that zeaxanthin purified from the rhodophyte Porphyridium purpureum (Bory) K.M.Drew &amp; R.Ross, Porphyridiaceae, promotes apoptosis in the A2058 human melanoma cell line expressing the oncogenic BRAF V600E mutation. Zeaxanthin 40 μM (IC50) induced chromatin condensation, nuclear blebbing, hypodiploidy, accumulation of cells in sub-G1 phase, DNA internucleosomal fragmentation and activation of caspase-3. Western blot analysis revealed that zeaxanthin induced up-regulation...
Tipo: Text
Palavras-chave: Cancer; Carotenoid; Melanoma; Microalgae; Phytoplankton; Zeaxanthin.
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00444/55595/57227.pdf