Consulta: creatorFacets:"Travnickova,Martina"
Registros recuperados: 1
Data/hora: 10/06/2024 08:16:51

Assessment of somaclonal variation in somatic embryo-derived plants of yacon [Smallanthus sonchifolius (Poepp. and Endl.) H. Robinson] using inter simple sequence repeat analysis and flow cytometry
Provedor de dados: Electronic Journal of Biotechnology
Autores: Viehmannova,Iva; Bortlova,Zuzana; Vitamvas,Jan; Cepkova,Petra Hlasna; Eliasova,Katerina; Svobodova,Eva; Travnickova,Martina.
Background Yacon (Smallanthus sonchifolius) is a root crop native to the Andean region. Low sexual reproductive capacity is a major constraint facing the genetic breeding of this crop. Biotechnological techniques offer alternative ways to widen genetic variability. We investigated somaclonal variation in regenerants of yacon derived from in vitro somatic embryogenesis using simple sequence repeat (ISSR) analysis and flow cytometry. Results Twenty tested ISSR primers provided a total of 7848 bands in 60 in vitro regenerants and control plant. The number of bands for each primer varied from 3 to 10, and an average of 6.95 bands was obtained per ISSR primer. Eight primers were polymorphic and generated 10 polymorphic bands with 7.19% mean polymorphism. ISSR...
Tipo: Journal article
Palavras-chave: DNA polymorphism; Molecular markers; Ploidy level; Proembryogenic callus.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000200008