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Diversity of enterovirus sequences detected in oysters by RT-heminested PCR
Provedor de dados: 5
Autores: Dubois, Eric; Merle, Ghislaine; Roquier, Catherine; Trompette, Aurélien; Le Guyader, Soizick; Cruciere, Catherine; Chomel, Jean-jacques.
Oysters harvested in western France, from five sites associated with outbreaks of food-borne norovirus gastroenteritis between February 2000 and March 2001, were assayed for enterovirus RNA by reverse transcriptase-heminested polymerase chain reaction (RT-heminested PCR). Forty percent (21/52) of shellfish samples (pool of seven oysters) were contaminated by enteroviruses. Infectious coxsackieviruses serotype A21 were isolated from three of these positive samples. Amplicons corresponding to 65 base sequences in the 5' untranslated region of the enteroviral genome were analyzed by direct sequencing. Interpretable results were obtained from 18 amplicons, but mixtures of sequences confused the results from 3 samples. Sequences isolated from samples from the...
Tipo: Text
Palavras-chave: Sequence analyze; RT PCR; Cell culture; Shellfish; Enterovirus.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-763.pdf