Consulta: creatorFacets:"Varsamos, S."
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Data/hora: 11/06/2024 04:05:22

Development of a new diagnostic tool (Mini-Array) for ostreid Herpesvrus 1 (OsHV-1) detection in the Pacific oyster Crassostre gigas
Provedor de dados: 5
Autores: Varsamos, S.; Pepin, Jean-francois; Sauvage, Christopher; Renault, Tristan.
OsHV-1 was the first herpesvirus to be identified in an invertebrate host and is associated to mortalities in the Pacific oyster (Crassostrea gigas) and other economically important bivalve species. Infections due to OsHV-1 are frequently reported and cause high mortality rates in larvae and spat of C. gigas. Detection of this pathogen is usually performed by PCR and/or in situ hybridization. This presentation will focus on the development of a new molecular diagnostic tool for OsHV-1 (and OsHV-1 variant 1) detection: the mini-array. This detection system, which allows multiple pathogens detection, combines viral DNA amplification by PCR and a specific hybridisation on a nylon membrane, revealed by a colorimetric reaction (DNA chip). Multiplex PCR involves...
Tipo: Text
Palavras-chave: Diagnostic; Crassotrea gigas; Oyster; Detection; Herpesviridae; OsHV 1.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3652.pdf