Consulta: creatorFacets:"Vasconcelos,Maria J. V. de"
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Data/hora: 09/06/2024 11:49:27

Identificação através de PCR dos genes CryI de cepas de Bacillus thuringiensis Berliner eficientes contra a lagarta do cartucho, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)
Provedor de dados: 44
Autores: Valicente,Fernando H.; Barreto,Marliton R.; Vasconcelos,Maria J. V. de; Figueiredo,José E.F. de; Paiva,Edilson.
Fall armyworm, Spodoptera frugiperda (J. E. Smith), is the most important maize insect pest in Brazil and its damage can reduce yield up to 34%. The objective of this work was to utilize the PCR technique to Identify Bacillus thuringiensis (B.t) strains that control S. frugiperda in maize crops. Bioassays showed that 16 strains were very effective against S. frugiperda, 15 of them collected from soil samples and one (T09) obtained at the Institute Pasteur. The DNA of these strains were probed with cryI general primers and their proteins were analised by SDS-PAGE eletrophoresis. All strains present positive results for cryI genes. To further characterize these strains, specific cryI primers were employed. PCR technique showed that some strains harbour the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; CryI genes; Biocontrol; Fall armyworm.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80592000000100018