Consulta: creatorFacets:"Vigneron, Vassilia"
Registros recuperados: 3
Data/hora: 12/06/2024 16:25:59

Detection of ostreid herpesvirus 1 (OsHV-1) DNA in seawater by PCR: influence of water parameters in bioassays
Provedor de dados: 5
Autores: Vigneron, Vassilia; Solliec, Gaelle; Montanie, Hélène; Renault, Tristan.
Since 1991, herpesvirus infections have been reported among larvae and juveniles of various bivalves. Most of the studies focused on detection of viral infections of economically important species. However, the persistence of bivalve herpesviruses; in the marine environment is poorly documented. The present study concerns the role of seawater parameters in Ostreid Herpesvirus I (OsHV-1) detection by polymerase chain reaction (PCR). Viral DNA extracted from purified particles or virions present in infected oyster larvae were detected by PCR after storage in different media at different temperatures. The lowest detection threshold was found using distilled water or Tris EDTA buffer. In seawater, the threshold was higher. The use of sterile media permitted...
Tipo: Text
Palavras-chave: Temperature; PCR; Detection; Seawater; Viral DNA; Oyster herpesvirus 1.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-2909.pdf

Détection et étude de la stabilité de l'ADN de virus de type herpès infectant les huîtres dans des échantillons d'eau
Provedor de dados: 5
Autores: Vigneron, Vassilia.
Depuis 1991, des mortalités massives associées à des infections à herpès virus sont rapportées chez des larves et des juvéniles de différentes espèces de bivalves dans différentes régions du globe. Les études ont porté essentiellement sur la mise en évidence de la présence de ce type de virus chez les coquillages d'intérêt économique. Actuellement, l'intérêt se porte sur la persistance du virus dans le milieu marin, et cela dans le but de déterminer si le milieu constitue ou non un moyen de contamination des animaux. Le travail a constitué en une étude sur la persistance de la détection de l'ADN d'herpès virus (OsHV-1) afin de mieux appréhender les modes de dissémination et de transmission du virus. Dans un premier temps, la stabilité de l'ADN purifié et...
Tipo: Text
Palavras-chave: Herpèsvirus; Eau de mer; Détection; PCR; Persistance; Stabilité.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00032/14350/11634.pdf

Recherches d'ADN d'hepès virus infectant les mollusques dans les populations d'huîtres en milieu naturel et dans leur environnement
Provedor de dados: 5
Autores: Pepin, Jean-francois; Solliec, Gaelle; Vigneron, Vassilia; Thebault, Anne; Lamothe, Julien; Renault, Tristan.
The presence of a herpes-type virus has been demonstrated in various species of marine shellfish (bivalves and gastropoda) throughout different regions of the world (Europe, North and South America, Asia, Australia), and is frequently associated with high mortality levels. Nevertheless, apart from the sanitary monitoring in mollusc farming, very little data is available about the detection of this type of virus in the natural populations of bivalves and about the persistence of such viruses in sea water. Various diagnostic tools have been developed in order to detect the oyster herpes virus (Ostreid herpesvirus, OsHV). The presence of this virus has been looked for by PCR in marine water samples coming from fattening ponds, in order to precise the natural...
Tipo: Text
Palavras-chave: Levures; Eau de mer; Diagnostic; Huîtres; Mollusques; OsHV; Herpèsvirus.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-3716.pdf