Consulta: creatorFacets:"Wabete, Nelly"
Registros recuperados: 19
Data/hora: 08/06/2024 13:47:29

DEDUCTION : A research project for shrimp farming sustainability in New-Caledonia
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Beliaeff, Benoit; Chim, Liet; Della Patrona, Luc; Goyard, Emmanuel; Herlin, Jose; Labreuche, Yannick; Walling, Emilie; Ansquer, Dominique; Brun, Pierre; Castex, Mathieu; Coatanea, Denis; Courties, Claude; De Lorgeril, Julien; Dufour, Robert; Frappier, Julie; Goarant, Cyrille; Huber, Matthias; Lemaire, Pierrette; Lemonnier, Hugues; Loubersac, Lionel; Le Roux, Frederique; Lucas, Ronan; Patrois, Jacques; Peignon, Jean-marie; Pham, Dominique; Ramage, Yannick; Soulard, Benoit; Vic, Marlene; Vourey, Elodie; Wabete, Nelly.
New Caledonian shrimp farming feasibility studies started in the mid-seventies. Today ca. 2000 tons of Litopenaeus stylirostris, introduced in 1978, are semi-intensively produced each year. Since fifteen years, production has been impacted by two seasonal vibriosis, the “Summer” syndrome caused by Vibrio nigripulchritudo in relation to pond bottom and water column quality, and the “Winter Syndrome” caused by Vibrio penaeicida in relation to drastic temperature drops . With the ambition of elucidating processes possibly leading to mortality outbreaks in ponds, Ifremer has been conducting a research program since 2003 under the DESANS project (2003-2006) and the present DEDUCTION project, equally funded by the South and North Provinces and by the Government...
Tipo: Text
Palavras-chave: Shrimp farming; Technical and scientific support; Vibriosis; Water quality; Pathology; Ecophysiology; Genetics; Zoosanitary monitoring and procedures; Multifactorial and multidisciplinary approach.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00143/25438/23591.pdf

Oxidative stress studies applied to the farmed shrimp Litopenaus stylirostris in new caledonia
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Chim, Liet; Castex, Mathieu; Lemaire, Pierrette; Wabete, Nelly.
The study of the antioxidant status and oxidative stress in shrimp L. stylirostris make it possible to assess risk factors (temperature, shrimp size, pathogen, ..) associated with shrimp farming. From a practical view, this research should lead to zootechnical and/or nutritional recommendations to improve the antioxidant status of the shrimp during their growout stage in farm.
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00197/30875/29243.pdf

Salinité et confort physiologique - Application pratique en élevage larvaire
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pham, Dominique; Mailliez, Jean-rene; Peignon, Jean-marie; Broutoi, Francis; Marteau, Anne-laure; Wabete, Nelly.
The caledonian blue shrimp Litopenaeus stylirostris is the second largest export commodity in New Caledonia. This industry is based on 19 production farms but also four hatcheries, two feed production units and two packing plants. The maximum production was reached in 2005 with 2400 tons. Supply of juveniles by hatchery is the bottle neck of the production chain because this species is not present in the natural environment. Recent problems of larval survival led some scientists to work on eco-physiology to propose adaptations of larval rearing protocol. Salinity of the rearing environment is taken as an example to study the influence of abiotic parameters on the animal physiology according to its development stage. The influence of salinity on survival...
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00115/22658/20603.pdf

Whole-Genome Sequence of   Pseudoalteromonas   sp. NC201, a Probiotic Strain for   Litopenaeus stylirostris   Hatcheries in New Caledonia
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Sorieul, Louis; Rückert, Christian; Al-dilaimi, Arwa; Winkler, Anika; Kalinowski, Jörn; Pham, Dominique; Wabete, Nelly; Boulo, Viviane.
The marine bacterium Pseudoalteromonas sp. strain NC201 has shown probiotic potential in Litopenaeus stylirostris rearing. In this study, the complete genome of NC201 was sequenced. This genome consists of a chromosome (4.13 Mb) and a chromid (1.24 Mb). The genome contains gene clusters coding for antibacterial peptides and secondary metabolites.
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00512/62329/66586.pdf

Effect of dietary probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress status of shrimp Litopenaeus stylirostris
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Castex, Mathieu; Lemaire, Pierrette; Wabete, Nelly; Chim, Liet.
in this study we evaluated the effects of the probiotic Pediococcus acidilactici MA18/5M on shrimp, Litopenaeus stylirostris (also called Penaeus stylirostris), first on antioxidant defences and secondly on the oxidative stress status in the shrimps' haemolymph and digestive gland. We conducted two experiments with the same protocol in which shrimps were fed two diets for three weeks: a control diet and a probiotic diet containing 1 g of live P. acidilactici MA18/5M kg(-1). In the first experiment, the shrimps were found to be healthy over the trial period; no mortalities and non-detectable signs of infection were recorded. These resulted in high final survival rates (above 90% in both treatments). On the other hand, during the second trial, carried out at...
Tipo: Text
Palavras-chave: Vibriosis; Antioxidant defences; Oxidative stress; Probiotic; Shrimps.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6780.pdf

Selection and characterization of potential probiotic bacteria for Litopenaeus stylirostris shrimp hatcheries in New Caledonia
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pham, Dominique; Ansquer, Dominique; Chevalier, Anne; Dauga, Clement; Peyramale, Aude; Wabete, Nelly; Labreuche, Yannick.
In New Caledonia, shrimp hatcheries are confronted with mass mortality in the larval stages, a phenomenon poorly understood as no specific causative agent has been identified. This has resulted in an excessive use of prophylactic antibiotics, although their adverse effects in aquaculture are notorious. The present work was thus aimed at selecting potential probiotic strains for penaeid hatcheries.  From a pool of more than 400 marine bacterial isolates sampled from the local marine environment seven strains exhibited in vitro antagonistic activity towards Vibrio harveyi. These isolates were characterized both phenotypically and genotypically using a biochemical approach and 16S rDNA sequencing. Six out of these seven strains were found to belong to the...
Tipo: Text
Palavras-chave: Shrimp; Litopenaeus stylirostris; Probiotic; Hatchery; Pseudoalteromonas; Immune response.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00190/30129/28591.pdf

Salinity tolerance, ontogeny of osmoregulation and zootechnical improvement in the larval rearing of the Caledonian Blue Shrimp, Litopenaeus stylirostris (Decapoda, Penaeidae)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pham, Dominique; Charmantier, Guy; Wabete, Nelly; Boulo, Viviane; Broutoi, Francis; Mailliez, Jean-rene; Peignon, Jean-marie; Charmantier-daures, Mireille.
The ontogeny of osmoregulationwas investigated in Litopenaeus stylirostris by studying salinity tolerance and osmoregulatory capacity. Shrimp at different larval and postlarval stages were exposed to various salinities and survival was monitored for 24 h. Survival rates exceeded 80% at salinity over 25 ppt (750 mOsm.kg(-1)) at all the stages. At salinities below to 25 ppt, salinity tolerance was higher in nauplii and zoeae than in mysis larvae. Postlarval stages were able to withstand lower salinities, e.g. 6.0 ppt (176 mOsm.kg(-1)) at PL9 stage, but they were more sensitive than larvae to salinities over 35 ppt (1035 mOsm.kg(-1)). Zoea and mysis larvae slightly hyper-regulated at all tested salinities. After metamorphosis, postlarvae progressively...
Tipo: Text
Palavras-chave: Penaeid; Litopenaeus stylirostris; Ontogeny; Osmoregulation; Growth.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00107/21843/20058.pdf

Live preys in shrimp culture: nutritional and sanitary considerations on the use of Artemia in New Caledonia
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pham, Dominique; Wabete, Nelly; Maillez, Jean-rene; Broutoi, Francis; Chim, Liet.
In aquaculture, Artemia nauplii are commonly used as live prey; and represent 40 % of the total aquaculture feed demands in the early stages. However, the production of Brine shrimp is very unstable from one year to another and in the late 90’s, only 20 % of the world aquaculture need was covered. An insufficient offer leads to higher prices of the Artemia cysts and consequently, the price of shrimp juveniles from hatchery. In New Caledonia, marine aquaculture is mainly based on the blue shrimp Litopenaeus stylirostris. From Zoea 3 to 10 days old post-larvae (P10), the shrimp are mainly fed with live preys. Eleven kilos of Artemia cyst are necessary for producing one million of P10.  From a sanitary point of view, Artemia can be a virus carrier and Vibrio...
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00198/30877/29245.pdf

Probiotic P-acidilactici application in shrimp Litopenaeus stylirostris culture subject to vibriosis in New Caledonia
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Castex, Mathieu; Chim, Liet; Pham, Dominique; Lemaire, Pierrette; Wabete, Nelly; Nicolas, Jean-louis; Schmidely, P; Mariojouls, C.
We studied the effects of a lactic acid bacterium, Pediococcus acidilactici (strain MA 18/5M, CNCM), as a dietary probiotic on growth performance and some nutritional and microbiological aspects of the shrimp Litopenaeus stylirostris. Experimental shrimp culture was carried out over 10 weeks, using floating cages of 14 m(2) each set in earthen ponds, in a New Caledonia farm affected by "Summer syndrome", a septicaemic vibriosis caused by Vibrio nigripulchritudo. The experiment design testing probiotic-coated pellets against control was replicated in two earthen ponds. High mortalities, characteristic of Summer syndrome, were observed during the first 2 weeks of the trial. The probiotic improved production in the treated cages from both ponds with increases...
Tipo: Text
Palavras-chave: Vibriosis; Floating cages; Litopenaeus stylirostris; Pediococcus acidilactici; Probiotic.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4079.pdf

Ontogeny of osmoregulation in the Pacific blue shrimp, Litopenaeus stylirostris (decapoda, penaeidae): Deciphering the role of the Na+/K+-ATPase
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pham, Dominique; Charmantier, Guy; Boulo, Viviane; Wabete, Nelly; Ansquer, Dominique; Dauga, Clement; Grousset, Evelyse; Labreuche, Yannick; Charmantier-daures, Mireille.
The role of the main ion transporting enzyme Na +/K + − ATPase in osmoregulation processes was investigated in Litopenaeus stylirostris. The development and localization of the osmoregulation sites were studied during ontogenesis by immunodetection of Na+ K+-ATPase using monoclonal antibodies and transmission electron microscopy (TEM). Osmoregulation sites were identified as the pleurae and branchiostegites in the zoeae and mysis stages. In the subsequent post-metamorphic stages the osmoregulatory function was mainly located in the epipodites and branchiostegites and osmotic regulation was later detected in the gills. The presence of ionocytes and microvilli in these tissues confirmed their role in ionic processes. The complete open reading frame of the...
Tipo: Text
Palavras-chave: Penaeid; Litopenaeus stylirostris; Ontogeny; Osmoregulation; Na+/K+-ATPase; RNA interference.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00311/42176/41477.pdf

Effect of probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress of Litopenaeus stylirostris under Vibrio nigripulchritudo challenge
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Castex, Mathieu; Lemaire, Pierrette; Wabete, Nelly; Chim, Liet.
Antioxidant defences and induced oxidative stress tissue damage of the blue shrimp Litopenaeus stylirostris, under challenge with Vibrio nigriputchritudo, were investigated for a 72-h period. For this purpose, L stylirostris were first infected by immersion with pathogenic V. nigripulchritudo strain SFn1 and then antioxidant defences: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (Gpx), Total antioxidant status (TAS), glutathiones and induced tissue damage (MDA and carbonyl proteins) were determined in the digestive gland at 0, 12, 24, 48 and 72 h post-infection (h.p.i.). In the meantime, TAS was also measured in the blood. Infection level of the shrimps during the challenge was followed by determining V. nigripulchritudo prevalence...
Tipo: Text
Palavras-chave: Probiotic; Pediococcus acidilactici; Shrimp; Oxidative stress; Antioxidant status; Vibrio; Immersion challenge.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00003/11419/7984.pdf

Evaluation of floating cages as an experimental tool for marine shrimp culture studies under practical earthen pond condition
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Castex, Mathieu; Chim, Liet; Lemaire, Pierrette; Wabete, Nelly; Pham, Dominique.
Scientific and technical studies carried out under semi-intensive farming conditions, with large earthen ponds (Fig 1), to assess the effects of treatments on zootechnical results are highly complex due to the many logistical, economic and/or experimental constraints involved. In our study, we evaluated the use of floatingcages (Paquotte et al., 1998) immersed in earthen ponds as an experimental tool in order to design future powerful and economical expe-riments under semi-intensive farming conditions.
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00197/30872/29240.pdf

Floating cages as an experimental tool for shrimp culture studies: first attempts to check their reliability
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pham, Dominique; Castex, Mathieu; Wabete, Nelly; Lemaire, Pierrette; Brun, Pierre.
The first aim of this study was to evaluate on a technical basis the rearing of shrimps in floating cages set up in earthen ponds. Shrimps reared in and outside the cages presented comparable growths and survival rates. Then it was concluded that shrimp culture in floating cages did not present zootechnical biases comparing to pond culture. The second objective of this study was to assess the present method as a statistical point of view. We found that for expected differences of 20% to the control mean, 3 and 6 floating cages per treatment will be reasonable to determined statistical differences for growth parameters and survival. Furthermore, we showed a significant pond effect as regard as survival and growth between both sets of cages. This results...
Tipo: Text
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-4100.pdf

Three years of experimental and applied research on the use of dietary probiotic Pediococcus acidilactici in shrimp culture : overview of the main results
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Castex, Mathieu; Chim, Liet; Lemaire, Pierrette; Wabete, Nelly.
In recent years the biological control of diseases by environmental friendly methods such as probiotics has become an important subject of investigation in aquaculture research. Probiotics are live microorganisms which when administrated in adequate amounts confer a health benefit on the host . In the last 4 years, IFREMER in collaboration with Lallemand company, have carried out researchs in New Caledonia with the aim to assess the probiotic strain P. acidilactici MA 18/5M on farmed shrimp Litopenaeus stylirostris.This project started in 2005 demonstrated the beneficial effects of the probiotic Pediococcus acidilactici dietary supplementation on the nutrition and the health status of peneid shrimps and highlighted that its use as probiotic can be part of...
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00197/30873/29241.pdf

Contribution à l'amélioration des survies et performances de reproduction de L. stylirostris en saison fraîche en Nouvelle-Calédonie
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pham, Dominique; Wabete, Nelly; Lemaire, Pierrette; Mailliez, Jean-rene; Broutoi, Francis; Chim, Liet.
Since 1993, the New Caledonian shrimp industry has been faced with cold season mortality outbreaks of multifactorial origin. This pathology called “Syndrome 93” affects as well animals in grow-out ponds as broodstock after their transfer in maturation room, with up to 35% of mortality in 48 hours. From literature data and physiological studies on Litopenaeus stylirostris, several combinations of three abiotic parameters (temperature, salinity and feeding) have been tested during the breeders stocking period. This research allowed us to lower stress factors impact and to propose a transfer protocol which leads to an optimal physiological comfort of the animal. Results showed that survival rate is improved and spawning rates are twice higher. These findings...
Tipo: Text
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00118/22970/20800.pdf

Phénomène des branchies orange dans les élevages de crevettes en Nouvelle-Calédonie
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lemonnier, Hugues; Barri, Kiam; Pham, Dominique; Wabete, Nelly; Boulo, Viviane; Lignot, Jean-herve; Royer, Florence; Hubert, Morgane; Blockmans, Bernard; Pierrot, Thomas; Laugier, Thierry; Tostin, Nathalie.
L'aquaculture est une activité importante du paysage économique de la Nouvelle-Calédonie., La crevette Bleue du Pacifique, Litopenaeus stylirostris est aujourd’hui l’espèce cible, élevée en semi-intensif, dans des bassins de terre de 4 à 10 hectares. Une coloration orange prononcée au niveau des branchies, comparable à celle observée suite à un stress hypoxique alors qu’aucun déficit en oxygène n’est constaté, est apparue récemment engendrant une décote du produit à la vente et donc un risque économique supplémentaire pour cette filière déjà fragilisée par des mortalités à vibrioses. Le phénomène se déclare à un poids moyen des animaux compris entre 10 et 18 g pour une durée moyenne d’élevage de 70 jours. L'intensité de la couleur évolue avec le stade de...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00248/35909/34462.pdf

Evaluation of floating cages as an experimental tool for marine shrimp culture studies under practical earthen pond conditions
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Chim, Liet; Castex, Mathieu; Pham, Dominique; Brun, Pierre; Lemaire, Pierrette; Wabete, Nelly; Schmidely, P; Mariojouls, C.
The New Caledonia blue shrimp Litopenaeus stylirostris is commercially produced under semi-intensive rearing conditions. The size of the farming earthen ponds (5–10 ha) and the subsequent production constraints make it difficult to use them as experimental units for random experiments. Moreover, since every pond has its own characteristics, ponds' effects cannot be ruled out, thus making it hard to define true replicates. In order to design future experiments under conditions resembling those used in production, we evaluated the possible use of floating cages as experimental units with the aim of assessing treatment effects with a reasonable statistical power. To this end, two sets of floating cages were placed respectively in two different ponds in a...
Tipo: Text
Palavras-chave: Statistical power; Replicates; Pond experimentation; Floating cages; Litopenaeus stylirostris.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4363.pdf

A soft technology to improve survival and reproductive performance of Litopenaeus stylirostris by counterbalancing physiological disturbances associated with handling stress
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Wabete, Nelly; Chim, Liet; Pham, Dominique; Lemaire, Pierrette; Massabuau, J.
The consequences of handling stress (fishing, transfer, eyestalk ablation) on shrimp broodstock are poorly documented. The weakness of farmed shrimp, Litopenaeus stylirostris, during winter is a major problem in New Caledonia, because of seasonal climate (tropical-sub-temperate). The transfer of broodstock in winter from earthen outdoor ponds to indoor maturation tanks in the hatchery (T=20 degrees C, Salinity=35 parts per thousand, fed shrimp) usually leads, after 48 h, to high mortality (up to 70%). Eyestalk ablation to induce ovarian maturation in females leads to further mortality.   Starting from a background analysis of physiological disturbances (initial osmoregulatory imbalance) associated with handling stress (Wabete, N., Chim, L., Lernaire, P.,...
Tipo: Text
Palavras-chave: Eyestalk ablation; Spawning; Handling stress; Broodstock; Litopenaeus stylirostris; Shrimp.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1880.pdf

Projet Déduction 2007-2010. Bilan par action et annexes
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Beliaeff, Benoit; Laugier, Thierry; Chim, Liet; Coatanea, Denis; Della Patrona, Luc; Goyard, Emmanuel; Herlin, Jose; Labreuche, Yannick; Lemaire, Pierrette; Lemonnier, Hugues; Morvan, Patrice; Patrois, Jacques; Peignon, Jean-marie; Pham, Dominique; Soulard, Benoit; Wabete, Nelly; Walling, Emilie; Ansquer, Dominique; Castex, Mathieu; Lucas, Ronan; Reynaud, Yann; Ifremer, LEAD-NC,.
Ce document constitue le bilan détaillé des résultats du projet DEDUCTION (2007-2010) , action par action. Le projet DEDUCTION "DEveloppement DUrable de la Crevetticulture, Traitement de l’Information et Observatoire du système en Nouvelle-Calédonie" est un projet de recherche et de développement en soutien à la filière crevetticole néo-calédonienne. C'est un programme très horizontal et pluridisciplinaire en poursuivant et développant les ouvertures scientifiques, techniques et thématiques établies à la faveur de DESANS. Son ossature s’articule autour de 4 actions : 3 actions de recherche et une action transversale devant faciliter les interfaces entre la recherche et sa finalité, entre les chercheurs et les partenaires locaux (GFA, Provinces,...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00199/31006/35663.pdf