Consulta: creatorFacets:"Wanchai Yenpetch"
Registros recuperados: 2
Data/hora: 16/06/2024 10:30:44

การสกัดดีเอ็นเอจากข้าวโพดในระดับเล็กสำหรับทำพีซีอาร์
Provedor de dados: Thai Agricultural Research Repository
Autores: Wanchai Yenpetch; Chaba Jampatong; Sanon Suksatan.
This method for instant DNA extraction from fresh corn leaf based on a simple DNA extraction with CTAB modified from Doyle and Doyle (1987) in small scale. After extraction steps, the extracted DNA was checked the quality and quantity by using a spectrophotometer to measure the absorbance at A260/A280. The result showed the feasibility of this method which was confirmed by using PCR method. The PCR product showed an expected band. Without the addition of the phenol or 2-beta-mercaptoethanol, this method could properly use in routine laboratory where DNA is used for PCR detection. In addition, this method requires less plant tissue to extract that is useful for other plant experiments.
Palavras-chave: Corn; Leaf; DNA extraction; PCR; ข้าวโพด; ใบสด; ดีเอ็นเอ; การสกัด; พีซีอาร์; คุณภาพ; สเปคโตรโฟโตมิเตอร์; อัตราการดูดกลืนแสง.
Ano: 2010
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/4690

การปรับปรุงข้าวโพดสายพันธุ์แท้ให้ต้านทานโรคราน้ำค้างโดยวิธี Marker-assisted Selection
Provedor de dados: Thai Agricultural Research Repository
Autores: Chaba Jampatong; Sansern Jampatong; Wanchai Yenpetch; Sanon Suksatan; Anucha Mekarun; Athiwat Songpinich.
Downy mildew (DM), Peronosclerospora sorghi (Weston and Uppal) C.G. Shaw, is one of the most destructive diseases of maize (Zea mays L.) in Thailand.  Understanding the genetic basis of downy mildew resistance could increase the efficiency of breeding for disease resistant germplasm. The objectives of this study were to use the SSR primers associated with QTLs which produced major effects, umc1072 found in chromosome bin 5.07, and bnlg1867 found in chromosome bin 6.01 used to transfer resistance alleles to susceptible lines by marker-assisted selection. A backcross method was carried out in this research. Agron18 and Nei452006 were susceptible lines used as recurrent parent, Nei9008 was resistant line used as donor parent. The results indicated that...
Palavras-chave: Maize; Downy mildew disease; SSR primers; Marker-assisted selection; ข้าวโพด; โรคราน้ำค้าง; การปรับปรุงพันธุ์; พันธุ์แท้; ความต้านทานโรค.
Ano: 2010
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/4661