Consulta: creatorFacets:"Wang,H."
Registros recuperados: 10
Data/hora: 10/06/2024 03:31:42

PDK2 promotes chondrogenic differentiation of mesenchymal stem cells by upregulation of Sox6 and activation of JNK/MAPK/ERK pathway
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Wang,H.; Shan,X.B.; Qiao,Y.J.
This study was undertaken to clarify the role and mechanism of pyruvate dehydrogenase kinase isoform 2 (PDK2) in chondrogenic differentiation of mesenchymal stem cells (MSCs). MSCs were isolated from femurs and tibias of Sprague-Dawley rats, weighing 300-400 g (5 females and 5 males). Overexpression and knockdown of PDK2 were transfected into MSCs and then cell viability, adhesion and migration were assessed. Additionally, the roles of aberrant PDK2 in chondrogenesis markers SRY-related high mobility group-box 6 (Sox6), type ΙΙ procollagen gene (COL2A1), cartilage oligomeric matrix protein (COMP), aggrecan (AGC1), type ΙX procollagen gene (COL9A2) and collagen type 1 alpha 1 (COL1A1) were measured by quantitative reverse-transcription polymerase chain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pyruvate dehydrogenase kinase isoform 2; Chondrogenic differentiation; Mesenchymal stem cell; SRY-related high mobility group-box 6; C-Jun N-terminal kinase (JNK).
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200606

Pim-1 kinase inhibits the activation of reporter gene expression in Elk-1 and c-Fos reporting systems but not the endogenous gene expression: an artifact of the reporter gene assay by transient co-transfection
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Yan,B.; Wang,H.; Kon,T.; Li,C.-Y.
We have studied the molecular mechanism and signal transduction of pim-1, an oncogene encoding a serine-threonine kinase. This is a true oncogene which prolongs survival and inhibits apoptosis of hematopoietic cells. In order to determine whether the effects of Pim-1 occur by regulation of the mitogen-activated protein kinase pathway, we used a transcriptional reporter assay by transient co-transfection as a screening method. In this study, we found that Pim-1 inhibited the Elk-1 and NFkappaB transcriptional activities induced by activation of the mitogen-activated protein kinase cascade in reporter gene assays. However, Western blots showed that the induction of Elk-1-regulated expression of endogenous c-Fos was not affected by Pim-1. The phosphorylation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pim-1 kinase; Signal transduction; Mitogen-activated protein kinase pathway; Elk-1; Reporter assay.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200002

SIRT1 negatively regulates amyloid-beta-induced inflammation via the NF-κB pathway
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Cao,L.; Liu,C.; Wang,F.; Wang,H.
Chronic inflammation induced by amyloid-beta (Aβ) plays a key role in the development of age-related macular degeneration (AMD), and matrix metalloproteinase-9 (MMP-9), interleukin (IL)-6, and IL-8 may be associated with chronic inflammation in AMD. Sirtuin 1 (SIRT1) regulates inflammation via inhibition of nuclear factor-kappa B (NF-κB) signaling, and resveratrol has been reported to prevent Aβ-induced retinal degeneration; therefore, we investigated whether this action was mediated via activation of SIRT1 signaling. Human adult retinal pigment epithelial (RPE) cells were exposed to Aβ, and overactivation and knockdown of SIRT1 were performed to investigate whether SIRT1 is required for abrogating Aβ-induced inflammation. We found that Aβ-induced RPE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amyloid-beta; SRT1720; Age-related macular degeneration; Barrier integrity; Tight junction; Matrix metalloproteinase-9.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800659

Effects of heme oxygenase-1 recombinant Lactococcus lactis on the intestinal barrier of hemorrhagic shock rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Gao,X.Y.; Zhou,X.F.; Wang,H.; Lv,N.; Liu,Y.; Guo,J.R.
This study aimed to investigate the effects of heme oxygenase-1 recombinant Lactococcus lactis (LL-HO-1) on the intestinal barrier of rats with hemorrhagic shock. One hundred Sprague-Dawley male rats (280–320 g) were randomly divided into healthy control group (N group) and hemorrhagic shock group (H group). Each group was subdivided into HO1t, HO2t, HO3t, PBS and LL groups in which rats were intragastrically injected with LL-HO-1 once, twice and three times, PBS and L. lactis (LL), respectively. The mortality, intestinal myeloperoxidase (MPO) activity, intestinal contents of TNF-α, IL-10 and HO-1, and intestinal Chiu's score were determined. Results showed that in N group, the HO-1 content increased after LL-HO-1 treatment, and significant difference was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heme oxygenase-1; Hemorrhagic shock; Lactococcus lactis; Intestinal barrier; Intestinal inflammation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700601

Astragalus polysaccharides improve cardiomyopathy in STZ-induced diabetic mice and heterozygous (SOD2+/-) knockout mice
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ju,J.; Chen,W.; Lai,Y.; Wang,L.; Wang,H.; Chen,W.J.; Zhao,X.; Ye,H.; LI,Y.; Zhang,Y.
Oxidative stress plays an important role in the development of diabetic cardiomyopathy. In the present study, we determined whether the effect of astragalus polysaccharides (APS) on diabetic cardiomyopathy was associated with its impact on oxidative stress. Streptozotocin (STZ)-induced diabetic mice and heterozygous superoxide dismutase (SOD2+/-) knockout mice were administered APS. The hemodynamics, cardiac ultrastructure, and the apoptosis, necrosis and proliferation of cardiomyocytes were assessed to evaluate the effect of APS on diabetic and oxidative cardiomyopathy. Furthermore, H2O2 formation, oxidative stress/damage, and SOD activity in cardiomyocytes were evaluated to determine the effects of APS on cardiac oxidative stress. APS therapy improved...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Cardiomyopathy; Oxidative stress; Superoxide dismutase.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800601

Meta-analysis of the efficacy of proton pump inhibitors for the symptoms of laryngopharyngeal reflux
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Liu,C.; Wang,H.; Liu,K.
The objective of this study was to perform a systematic review and meta-analysis to assess the effectiveness of proton pump inhibitors (PPI) for reflux disease in adult patients with laryngopharyngeal symptoms. A comprehensive search of Cochrane Library, EMBASE, Ovid EBM Reviews, and PubMed was performed for English-language literature about laryngopharyngeal reflux (LPR), in September 2014. The papers were filtered using pre-defined inclusion and exclusion criteria. Eight papers were identified and included in this meta-analysis. The sample comprised a pooled total of 370 patients, of which 210 and 160 patients took PPIs and placebo, respectively. The difference between PPIs and placebo groups in overall improvement of symptoms in adult patients with LPR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastroesophageal reflux disease; Adult; Proton pump inhibitors; Meta-analysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700704

Efficacy of minimally invasive tonsil surgery for treatment of obstructive sleep apnea-hypopnea syndrome in children
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,X.-Q.; Wang,H.; Zhou,J.; Zeng,P.; Zhao,Y.; Zhang,Y.; Liu,C.; Jiang,L.-Q.; Lan,Y.-J.
This study aimed to investigate the efficacy of minimally invasive tonsil surgery for the treatment of obstructive sleep apnea-hypopnea syndrome (OSAHS) in children. Tonsil ablation or turbinate reduction was performed on 49 pediatric patients with OSAHS by minimally invasive tonsil surgery. In order to evaluate the efficacy of surgery, a comparison was conducted between pre-operation and post-operation data in terms of the symptoms, signs and polysomnography test. Total effectiveness rate of the surgery was 83.7%. Subgroup analysis was also performed based on the severity of their conditions: mild, moderate, and severe groups had an effectiveness rate of 90.0, 88.9, and 66.7%, respectively (Hc=6.665, P&lt;0.05). Postoperatively, the apnea-hypopnea index,...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Tonsil; Children; Obstructive sleep apnea-hypopnea syndrome; Polysomnography; Surgery.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500703

Histopathological characteristics of a novel knock-in mouse prostate cancer model
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Wu,G.; Wang,D.; Wang,H.; Yuan,J.; Xuan,J.W.
Prostate cancer is relatively unique to man. There is no naturally occurring prostate cancer in the mouse. Pre-clinical studies involve the establishment of a genetically engineered mouse prostate cancer model with features close to those of the human situation. A new knock-in mouse adenocarcinoma prostate (KIMAP) model was established, which showed close-to-human kinetics of tumor development. In order to determine if the similar kinetics is associated with heterogeneous tumor architecture similar to the human situation, we utilized a new mouse histological grading system (Gleason analogous grading system) similar to the Gleason human grading system and flow cytometry DNA analysis to measure and compare the adenocarcinoma of the KIMAP model with human...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer model; KIMAP mouse model; Gleason grading system; Prostate adenocarcinoma.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600008

Comparative efficacy and safety of contact force-sensing catheter and second-generation cryoballoon ablation for atrial fibrillation: a meta-analysis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhou,X.; Lv,W.; Zhang,W.; Ye,Y.; Li,Y.; Zhou,Q.; Zhang,J.; Xing,Q.; Lu,Y.; Zhang,L.; Wang,H.; Qin,W.; Tang,B.
This meta-analysis compared the efficacy and safety of the contact force (CF)-sensing catheter and second-generation cryoballoon (CB) ablation for treating atrial fibrillation (AF). Six controlled clinical trials comparing ablation for AF using a CF-sensing catheter or second-generation CB were identified from PubMed, EMBASE, Cochrane Library, Wanfang Data, and China National Knowledge Infrastructure. The procedure duration was significantly lower in the CB group compared with that in the CF group [mean difference (MD)=29.4; 95%CI=17.84–40.96; P=0.01], whereas there was no difference between the groups for fluoroscopy duration (MD=0.59; 95%CI=–4.48–5.66; P=0.82). Moreover, there was no difference in the incidence of non-lethal complications (embolic event,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atrial fibrillation; Ablation; Contact force-sensing catheter; Second-generation cryoballoon; Meta-analysis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900607

Serum microRNA-30c levels are correlated with disease progression in Xinjiang Uygur patients with chronic hepatitis B
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,J.; Ma,J.; Wang,H.; Guo,L.; Li,J.
We aimed to investigate the potential role and mechanism of microRNA-30c (miR-30c) in the pathological development of chronic hepatitis B (CHB). The serum levels of miR-30c in hepatitis B virus (HBV) carrier Xinjiang Uygur patients with inactive, low-replicative, high-replicative and HBe antigen-positive CHB were investigated. HepG2 cells were co-transfected with pHBV1.3 and miR-30c mimic or inhibitor or scramble RNA. The effects of miR-30c dysregulation on HBV replication and gene expression, cell proliferation and cell cycle were then investigated. miR-30c was down-regulated in Xinjiang Uygur patients with CHB compared to healthy controls and its expression level discriminated HBV carrier patients with inactive, low-replicative, high-replicative and HBe...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic hepatitis B; Disease progression; MicroRNA-30c; Hepatitis B virus replication; Cell proliferation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600601