Consulta: creatorFacets:"Wang,Linlin"
Registros recuperados: 2
Data/hora: 10/06/2024 13:35:42

bFGF promotes adipocyte differentiation in human mesenchymal stem cells derived from embryonic stem cells
Provedor de dados: 74
Autores: Song,Xinghui; Li,Yanwei; Chen,Xiao; Yin,Guoli; Huang,Qiong; Chen,Yingying; Xu,Guowei; Wang,Linlin.
In this work we describe the establishment of mesenchymal stem cells (MSCs) derived from embryonic stem cells (ESCs) and the role of bFGF in adipocyte differentiation. The totipotency of ESCs and MSCs was assessed by immunofluorescence staining and RT-PCR of totipotency factors. MSCs were successfully used to induce osteoblasts, chondrocytes and adipocytes. MSCs that differentiated into adipocytes were stimulated with and without bFGF. The OD/DNA (optical density/content of total DNA) and expression levels of the specific adipocyte genes PPARγ2 (peroxisome proliferator activated receptor γ2) and C/EBPs were higher in bFGF cells. Embryonic bodies had a higher adipocyte level compared with cells cultured in plates. These findings indicate that bFGF promotes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryonic stem cells; Mesenchymal stem cells; Fibroblast growth factor; Adipocyte differentiation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100019

lncRNA OIP5-AS1 targets ROCK1 to promote cell proliferation and inhibit cell apoptosis through a mechanism involving miR-143-3p in cervical cancer
Provedor de dados: 56
Autores: Song,Linlin; Wang,Linlin; Pan,Xiaoli; Yang,Caihong.
Opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) is one kind of cytoplasmic long non-coding RNA (lncRNA), which has been demonstrated to play a critical function in multiple cancers. However, the detailed mechanism of OIP5-AS1 in the regulation of cervical cancer progression is still obscure. Here, we demonstrated that lncRNA OIP5-AS1 was upregulated in cervical cancer and was correlated with poor prognosis by bioinformatics studies. OIP5-AS1 depletion inhibited cell proliferation and promoted cell apoptosis in cervical cancer cells. Furthermore, we clarified that ROCK1 was the downstream effector of OIP5-AS1 and OIP5-AS1 acted as a molecular sponge of miR-143-3p. Finally, we verified that OIP5-AS1 exerted its function in the regulation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LncRNA; OIP5-AS1; Cervical cancer; MiR-143-3p; ROCK1.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100608