Consulta: creatorFacets:"Wang,Wen-wen"
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Anti-Trimeresurus albolabris venom IgY antibodies: preparation, purification and neutralization efficacy
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Duan,Hai-long; He,Qi-yi; Zhou,Bin; Wang,Wen-wen; Li,Bo; Zhang,Ying-zheng; Deng,Qiu-ping; Zhang,Ying-feng; Yu,Xiao-dong.
Abstract  Background  Snakebite incidence in southwestern China is mainly attributed to one of the several venomous snakes found in the country, the white-lipped green pit viper Trimeresurus albolabris. Since antivenom produced from horses may cause numerous clinical side effects, the present study was conducted aiming to develop an alternative antivenom antibody (immunoglobulin Y - IgY) from leghorn chickens.  Methods  IgY in egg yolk from white leghorn chicken previously injected with T. albolabris venom was extracted by water, precipitated by ammonium sulfate and purified by affinity chromatographic system. IgY was identified by SDS-PAGE, ELISA and Western blot, and its neutralizing assay was conducted on mice.  Results  Chickens injected multiple times...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IgY; Egg yolk; Snake venom; Trimeresurus albolabris; LD50.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100316