Consulta: creatorFacets:"Wen,Yu"
Registros recuperados: 2
Data/hora: 10/06/2024 15:13:55

BC047440 antisense eukaryotic expression vectors inhibited HepG2 cell proliferation and suppressed xenograft tumorigenicity
Provedor de dados: 56
Autores: Zheng,Lu; Liang,Ping; Zhou,JianBo; Huang,XiaoBing; Wen,Yu; Wang,Zheng; Li,Jing.
The biological functions of the BC047440 gene highly expressed by hepatocellular carcinoma (HCC) are unknown. The objective of this study was to reconstruct antisense eukaryotic expression vectors of the gene for inhibiting HepG2 cell proliferation and suppressing their xenograft tumorigenicity. The full-length BC047440 cDNA was cloned from human primary HCC by RT-PCR. BC047440 gene fragments were ligated with pMD18-T simple vectors and subsequent pcDNA3.1(+) plasmids to construct the recombinant antisense eukaryotic vector pcDNA3.1(+)BC047440AS. The endogenous BC047440 mRNA abundance in target gene-transfected, vector-transfected and naive HepG2 cells was semiquantitatively analyzed by RT-PCR and cell proliferation was measured by the MTT assay. Cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma; BC047440; Antisense eukaryotic expression vector; Cell proliferation; Tumorigenicity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200002

Farmer field school’s training on knowledge level of citrus growers regarding improved production practices
Provedor de dados: 65
Autores: Victor,Akash Nougha; Luqman,Muhammad; Shiwei,Xu; Wen,Yu; Majeed,Muhammad Zeeshan.
ABSTRACT: The major objective of this paper was to assess the impact of the Farmer field school (FFS) training program on the knowledge level of farmers (citrus growers) regarding improved citrus production practices. Distract Sargodha was the targeted research area for this study being the leading producer of citrus in Pakistan. Quantitative data were collected through interview schedule and analyzed through Statistical Package for Social Sciences (SPSS). It was conclude that young people were more tend towards getting training under FFS program than old age farmers. Educated farmers were in majority in FFS farmers while; conversely illiterate farmers were in majority in the category of non-FFs farmers. In category of FFS farmers livestock farming was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Farmer field school; Agricultural extension; Integrated pest management; Citrus production practices.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001000931