Consulta: creatorFacets:"Wilson,R.A."
Registros recuperados: 3
Data/hora: 03/06/2024 03:49:12

Cytokines as determinants of resistance and pathology in human Schistosoma mansoni infection
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Corrêa-Oliveira,R.; Malaquias,L.C.C.; Falcão,P.L.; Viana,I.R.C.; Bahia-Oliveira,L.M.G.; Silveira,A.M.S.; Fraga,L.A.O.; Prata,A.; Coffman,R.L.; Lambertucci,J.R.; Cunha-Melo,J.R.; Martins-Filho,O.A.; Wilson,R.A.; Gazzinelli,G.
The role of different cytokines in the peripheral blood mononuclear cell (PBMC) proliferative response and in in vitro granuloma formation was evaluated in a cross-sectional study with patients with the different clinical forms and phases of Schistosoma mansoni infection, as well as a group of individuals "naturally" resistant to infection named normal endemic (NE). The blockage of IL-4 and IL-5 using anti-IL-4 and anti-IL-5 antibodies significantly reduced the PBMC proliferative response to soluble egg (SEA) and adult worm (SWAP) antigens in acute (ACT), chronic intestinal (INT) and hepatosplenic (HS) patients. Similar results were obtained in the in vitro granuloma formation. Blockage of IL-10 had no significant effect on either assay using PBMC from ACT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schistosoma mansoni human schistosomiasis mansoni cytokines IL-10 IL-4 IFN-&lt; FONT FACE=Symbol&gt; G&lt; /font&gt.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100024

Sm14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm14 protein within the adult worm
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Brito,C.F.A.; Oliveira,G.C.; Oliveira,S.C.; Street,M.; Riengrojpitak,S.; Wilson,R.A.; Simpson,A.J.G.; Correa-Oliveira,R.
Sm14 is a 14-kDa vaccine candidate antigen from Schistosoma mansoni that seems to be involved in cytoplasmic trafficking of fatty acids. Although schistosomes have a high requirement for lipids, they are not able to synthesize fatty acids and sterols de novo. Thus, they must acquire host lipids. In order to determine whether Sm14 is present in different stages of the life cycle of the parasite, we performed RT-PCR. Sm14 mRNA was identified in all stages of the life cycle studied, mainly schistosomulum, adult worm and egg. Additionally, we used a rabbit anti-Sm14 polyclonal antibody in an indirect immunofluorescence assay to localize Sm14 in adult worm sections. The basal lamella of the tegument and the gut epithelium were strongly labeled. These tissues...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sm14; Fatty acid-binding protein; FABP; Immunolocalization; RT-PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300014

Interferon gamma is a key cytokine in lung phase immunity to schistosomes but what is its precise role?
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Wilson,R.A.
Vaccination of mice with radiation-attenuated cercariae of Schistosoma mansoni induces a high level of protection against challenge with normal larvae. The immune effector mechanism, which operates in the lungs, is a cell-mediated delayed-type hypersensitivity response and involves the formation of a tight focus of mononuclear cells around embolised larvae. CD4+ T cells with Th1 characteristics are a major component of the infiltrate. They secrete abundant interferon gamma (IFN&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;) upon antigen stimulation in vitro, whilst in vivo neutralisation of the cytokine results in 90% abrogation of immunity. IFN&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; can induce a large number of genes and an attempt has been made to identify...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interferon gamma; Schistosoma mansoni; Lung phase immunity; Nitric oxide; Adhesion molecule.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100022