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Simultaneous analysis of microbial identity and function using NanoSIMS
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Li, T; Wu, T; Mazeas, L; Toffin, Laurent; Guerquin Kern, J; Leblon, G; Bouchez, T.
Identifying the function of uncultured microbes in their environments today remains one of the main challenges for microbial ecologists. In this article, we describe a new method allowing simultaneous analysis of microbial identity and function. This method is based on the visualization of oligonucleotide probe-conferred hybridization signal in single microbial cells and isotopic measurement using high-resolution ion microprobe (NanoSIMS). In order to characterize the potential of the method, an oligonucleotide containing iodized cytidine was hybridized on fixed cells of Escherichia coli cultured on media containing different levels of C-13 or N-15. Iodine signals could clearly be localized on targeted cells and the isotopic enrichment could be monitored...
Tipo: Text
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4553.pdf