Consulta: creatorFacets:"YAMAGUCHI,I. K."
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Data/hora: 01/06/2024 18:10:58

A SENSITIVE AND SPECIFIC IMMUNOASSAY FOR THE MEASUREMENT OF THE ANTIBODIES PRESENT IN HORSE ANTIVENOMS ENDOWED WITH THE CAPACITY TO BLOCK THE PHOSPHOLIPASE A2-DEPENDENT HEMOLYSIS INDUCED BY SNAKE VENOMS
Provedor de dados: Journal of Venomous Animals and Toxins
Autores: ROCHA CAMPOS,A. C. M.; FREITAS,M. C. W. DE; YAMAGUCHI,I. K.; MARCELINO,J. R.; DIAS DA SILVA,W.
Phospholipase A2 (PLA2), a component of most snake venom toxins, cleaves 3-sn-phosphoglycerides releasing lysophosphatidyl-choline. The indirect quantitative assay method for PLA2 was standardized for specific antivenom titration in a fast and sensitive assay by the similarity with the hemolysis induced by PLA2 and by complement system in sheep erythrocytes. The curves obtained by plotting the degree of hemolysis against the doses of snake venom are concave to the abscissa axis following an equation similar to that previously described for the hemolysis induced by the C system. We observed that venoms of some Bothrops, Crotalus and Micrurus species contained around 1 x 10&lt;img SRC="http:/img/fbpe/jvat/v2n2/image603.gif"&gt; to 10&lt;img...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PLA2; Snake venoms; Immunoassay; Antivenoms; Indirect hemolysis.
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301996000200004