Consulta: creatorFacets:"Zhang,J."
Registros recuperados: 13
Data/hora: 09/06/2024 14:54:49

Regulation of semaphorin 4D expression and cell proliferation of ovarian cancer by ERalpha and ERbeta
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Liu,Y.; Hou,Y.; Ma,L.; Sun,C.; Pan,J.; Yang,Y.; Zhou,H.; Zhang,J.
Ovarian cancer is one of the most common malignancies in women. Semaphorin 4D (sema 4D) is involved in the progress of multiple cancers. In the presence of estrogen-like ligands, estrogen receptors (ERα and ERβ) participate in the progress of breast and ovarian cancers by transcriptional regulation. The aim of the study was to investigate the role of sema 4D and elucidate the regulatory pattern of ERα and ERβ on sema 4D expression in ovarian cancers. Sema 4D levels were up-regulated in ovarian cancer SKOV-3 cells. Patients with malignant ovarian cancers had significantly higher sema 4D levels than controls, suggesting an oncogene role of sema 4D in ovarian cancer. ERα expressions were up-regulated in SKOV-3 cells compared with normal ovarian IOSE80...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Semaphorin 4D expression; ERα; ERβ; Ovarian cancer; Cell proliferation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300603

Long non-coding RNA CHCHD4P4 promotes epithelial-mesenchymal transition and inhibits cell proliferation in calcium oxalate-induced kidney damage
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,C.; Yuan,J.; Hu,H.; Chen,W.; Liu,M.; Zhang,J.; Sun,S.; Guo,Z.
Kidney stone disease is a major cause of chronic renal insufficiency. The role of long non-coding RNAs (lncRNAs) in calcium oxalate-induced kidney damage is unclear. Therefore, we aimed to explore the roles of lncRNAs in glyoxylate-exposed and healthy mouse kidneys using microarray technology and bioinformatics analyses. A total 376 mouse lncRNAs were differentially expressed between the two groups. Using BLAST, 15 lncRNA homologs, including AU015836 and CHCHD4P4, were identified in mice and humans. The AU015836 expression in mice exposed to glyoxylate and the CHCHD4P4 expression in human proximal tubular epithelial (HK-2) cells exposed to calcium oxalate monohydrate were analyzed, and both lncRNAs were found to be upregulated in response to calcium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney calculi; Long non-coding RNAs; Fibrosis; Cell proliferation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100610

BMP-2 and titanium particles synergistically activate osteoclast formation
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Sun,S.X.; Guo,H.H.; Zhang,J.; Yu,B.; Sun,K.N.; Jin,Q.H.
A previous study showed that BMP-2 (bone morphogenetic protein-2) and wear debris can separately support osteoclast formation induced by the receptor activator of NF-κB ligand (RANKL). However, the effect of BMP-2 on wear debris-induced osteoclast formation is unclear. In this study, we show that neither titanium particles nor BMP-2 can induce osteoclast formation in RAW 264.7 mouse leukemic monocyte macrophage cells but that BMP-2 synergizes with titanium particles to enhance osteoclast formation in the presence of RANKL, and that at a low concentration, BMP-2 has an optimal effect to stimulate the size and number of multinuclear osteoclasts, expression of osteoclast genes, and resorption area. Our data also clarify that the effects caused by the increase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone morphogenetic protein; Titanium; Osteoclasts; SMAD; RANKL.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600461

Inflammation induced by increased frequency of intermittent hypoxia is attenuated by tempol administration
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,J.; Zheng,L.; Cao,J.; Chen,B.; Jin,D.
The levels of serum inflammatory cytokines and the activation of nuclear factor kappa B (NF-κB) and hypoxia inducible factor-1α (HIF-1α) in heart tissues in response to different frequencies of intermittent hypoxia (IH) and the antioxidant tempol were evaluated. Wistar rats (64 males, 200-220 g) were randomly divided into 6 experimental groups and 2 control groups. Four groups were exposed to IH 10, 20, 30, or 40 times/h. The other 2 experimental groups were challenged with IH (30 times/h) plus tempol, either beginning on day 0 (IH30T0) or on day 29 (IH30T29). After 6 weeks of challenge, serum levels of tumor necrosis factor (TNF)-α, intracellular adhesion molecule (ICAM)-1, and interleukin-10 were measured, and western blot analysis was used to detect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obstructive sleep apnea; Intermittent hypoxia; Inflammation; Nuclear factor kappa B; Antioxidant.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201115

Galunisertib (LY2157299), a transforming growth factor-β receptor I kinase inhibitor, attenuates acute pancreatitis in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Liu,X.; Yu,M.; Chen,Y.; Zhang,J.
Galunisertib (LY2157299), a selective ATP-mimetic inhibitor of TGF-β receptor I (TGF-βRI), is the only known TGF-β pathway inhibitor. In the present study, we investigated the effect of galunisertib on taurocholate (TAC)-induced acute pancreatitis (AP) in rats, and the role of TGF-β and NF-κB signaling pathways. AP was induced by infusion of TAC into the pancreatic duct of Sprague-Dawley male rats (n=30). The rats (220±50 g) were administered galunisertib intragastrically [75 mg·kg-1·day-1 for 2 days (0 and 24 h)]. Serum IL-1β, IL-6, TNF-α, amylase (AMY), lipase (LIP), and myeloperoxidase (MPO) levels were measured by ELISA. NF-κB activity was detected by electrophoretic mobility shift assay (EMSA); NF-κBp65 and TGF-β1 proteins, as well as TGF-βRI and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute pancreatitis; TGFβ1; NF-κB; Galunisertib.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900705

Comparison of the neurobiological effects of attribution retraining group therapy with those of selective serotonin reuptake inhibitors
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Wang,C.; Zhang,N.; Zhang,Y.L.; Zhang,J.; Yang,H.; Timothy,T.C.
The aim of this study was to compare the effectiveness of attribution retraining group therapy (ARGT) with selective serotonin reuptake inhibitors (SSRIs) in the treatment of major depressive disorder (MDD), generalized anxiety disorder (GAD), and obsessive-compulsive disorder (OCD). Subjects were sequentially recruited and randomized into two groups, one receiving ARGT (n = 63) and the other SSRIs (n = 66) for 8 weeks. Fifty-four ARGT outpatients with MDD (n = 19), GAD (n = 19), and OCD (n = 16) and 55 SSRI outpatients with MDD (n = 19), GAD (n = 19), and OCD (n = 17) completed the study. All subjects were assessed using the Hamilton Depression Scale and Hamilton Anxiety Scale before and after treatment. The 10-item Yale-Brown Obsessive Compulsive Scale...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Attribution retraining group therapy; Selective serotonin reuptake inhibitors; Neurobiological effects; Serotonin; Cortisol.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300318

Obesity inversely correlates with prostate-specific antigen levels in a population with normal screening results of prostate cancer in northwestern China
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,J.; Ma,M.; Nan,X.; Sheng,B.
Serum prostate-specific antigen (PSA) is a diagnostic biomarker of prostate cancer and is possibly associated with obesity. This study aimed to explore the relationships between obesity indicators [body mass index (BMI) and waist circumference (WC)] with PSA in Chinese men. A cross-sectional study of men aged 30-85 years undergoing prostate cancer screening was conducted from August 2008 to July 2013 in Xi'an, China. Data were obtained from clinical reports, condition was recorded based on self-report including demographics, weight, height, and WC (&gt;90 cm=obese). Fasting blood glucose (FBG) and prostate volume (PV) were assessed clinically. Patients were grouped by BMI (normal=22.9, overweight=23-27.4, obese≥27.5 kg/m2). PSA parameters of density...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate-specific antigen; Body mass index; Obesity; Prostate cancer; Screening.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800704

Exogenous normal lymph reduces liver injury induced by lipopolysaccharides in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhao,Z.G.; Zhang,L.L.; Niu,C.Y.; Zhang,J.
The liver is one of the target organs damaged by septic shock, wherein the spread of endotoxins begins. This study aimed to investigate the effects of exogenous normal lymph (ENL) on lipopolysaccharide (LPS)-induced liver injury in rats. Male Wistar rats were randomly divided into sham, LPS, and LPS+ENL groups. LPS (15 mg/kg) was administered intravenously via the left jugular vein to the LPS and LPS+ENL groups. At 15 min after the LPS injection, saline or ENL without cell components (5 mL/kg) was administered to the LPS and LPS+ENL groups, respectively, at a rate of 0.5 mL/min. Hepatocellular injury indices and hepatic histomorphology, as well as levels of P-selectin, intercellular adhesion molecule 1 (ICAM-1), myeloperoxidase (MPO), and Na+-K+-ATPase,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exogenous normal lymph; Lipopolysaccharide; Liver injury; Intercellular adhesion molecule 1; Myeloperoxidase; Na+-K+-ATPase.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200128

Establishment of patient-derived tumor xenograft (PDTX) models using samples from CT-guided percutaneous biopsy
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhuang,Y.-P.; Zhu,Y.-P.; Wang,H.-Y.; Sun,L.; Zhang,J.; Hao,Y.-P.; Wang,L.
This study aimed to investigate the feasibility of the establishment of a human cancer xenograft model using samples from computed tomography (CT)-guided percutaneous biopsy. Fresh tumor tissues obtained from 10 cancer patients by CT-guided percutaneous biopsy were subcutaneously inoculated into NOD-Prkdcem26Il2rgem26Nju (NCG) mice to establish human patient-derived tumor xenograft (PDTX) models. The formation of first and second generation xenografts was observed, and tumor volume was recorded over time. Tumor tissue consistency between the PDTX model and primary tumors in patients was compared using H&amp;E staining and immunohistochemistry. Pharmacodynamic tests of clinically used chemotherapeutic drugs were conducted on second generation xenografts,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; Patient-derived tumor xenografts (PDTX); Computed tomography; X-ray; Percutaneous biopsy; Mouse models.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600705

Treatment of a subdural empyema complicated by intracerebral abscess due to Brucella infection
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,J.; Chen,Z.; Xie,L.; Zhao,C.; Zhao,H.; Fu,C.; Chen,G.; Hao,Z.; Wang,L.; Li,W.
A 55-year-old male presented with fever, stupor, aphasia, and left hemiparesis. A history of head trauma 3 months before was also reported. Cranial magnetic resonance imaging revealed slight contrast enhancement of lesions under the right frontal skull plate and right frontal lobe. Because of deterioration in nutritional status and intracranial hypertension, the patient was prepared for burr hole surgery. A subdural empyema (SDE) recurred after simple drainage. After detection of Brucella species in SDE, craniotomy combined with antibiotic treatment was undertaken. The patient received antibiotic therapy for 6 months (two doses of 2 g ceftriaxone, two doses of 100 mg doxycycline, and 700 mg rifapentine for 6 months) that resulted in complete cure of the...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Subdural empyema; Neurobrucellosis; Craniotomy; Infection; Treatment.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500501

High expression of the circadian gene mPer2 diminishes the radiosensitivity of NIH 3T3 cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Chang,L.; Liu,Y.Y.; Zhu,B.; Li,Y.; Hua,H.; Wang,Y.H.; Zhang,J.; Jiang,Z.; Wang,Z.R.
Period2 is a core circadian gene, which not only maintains the circadian rhythm of cells but also regulates some organic functions. We investigated the effects of mPeriod2 (mPer2) expression on radiosensitivity in normal mouse cells exposed to 60Co-γ-rays. NIH 3T3 cells were treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) to induce endogenous mPer2 expression or transfected with pcDNA3.1(+)-mPer2 and irradiated with 60Co-γ-rays, and then analyzed by several methods such as flow cytometry, colony formation assay, RT-PCR, and immunohistochemistry. Flow cytometry and colony formation assay revealed that irradiated NIH 3T3 cells expressing high levels of mPer2 showed a lower death rate (TPA: 24 h 4.3% vs 12 h 6.8% and control 9.4%; transfection:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Circadian; MPer2; Radiation; Cell death; Proliferation; DNA repair.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000003

Comparative efficacy and safety of contact force-sensing catheter and second-generation cryoballoon ablation for atrial fibrillation: a meta-analysis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhou,X.; Lv,W.; Zhang,W.; Ye,Y.; Li,Y.; Zhou,Q.; Zhang,J.; Xing,Q.; Lu,Y.; Zhang,L.; Wang,H.; Qin,W.; Tang,B.
This meta-analysis compared the efficacy and safety of the contact force (CF)-sensing catheter and second-generation cryoballoon (CB) ablation for treating atrial fibrillation (AF). Six controlled clinical trials comparing ablation for AF using a CF-sensing catheter or second-generation CB were identified from PubMed, EMBASE, Cochrane Library, Wanfang Data, and China National Knowledge Infrastructure. The procedure duration was significantly lower in the CB group compared with that in the CF group [mean difference (MD)=29.4; 95%CI=17.84–40.96; P=0.01], whereas there was no difference between the groups for fluoroscopy duration (MD=0.59; 95%CI=–4.48–5.66; P=0.82). Moreover, there was no difference in the incidence of non-lethal complications (embolic event,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atrial fibrillation; Ablation; Contact force-sensing catheter; Second-generation cryoballoon; Meta-analysis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900607

Serum microRNA-30c levels are correlated with disease progression in Xinjiang Uygur patients with chronic hepatitis B
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,J.; Ma,J.; Wang,H.; Guo,L.; Li,J.
We aimed to investigate the potential role and mechanism of microRNA-30c (miR-30c) in the pathological development of chronic hepatitis B (CHB). The serum levels of miR-30c in hepatitis B virus (HBV) carrier Xinjiang Uygur patients with inactive, low-replicative, high-replicative and HBe antigen-positive CHB were investigated. HepG2 cells were co-transfected with pHBV1.3 and miR-30c mimic or inhibitor or scramble RNA. The effects of miR-30c dysregulation on HBV replication and gene expression, cell proliferation and cell cycle were then investigated. miR-30c was down-regulated in Xinjiang Uygur patients with CHB compared to healthy controls and its expression level discriminated HBV carrier patients with inactive, low-replicative, high-replicative and HBe...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic hepatitis B; Disease progression; MicroRNA-30c; Hepatitis B virus replication; Cell proliferation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600601