Consulta: creatorFacets:"Zhang,L."
Registros recuperados: 14
Data/hora: 10/06/2024 14:03:41

Prevalence of bovine tuberculosis in the Aksu Region of Xinjiang, China, between 1985 and 2016
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Liu,Y.H.; Kang,Q.; Yang,B.; Li,F.; Li,X.Q.; Zhang,L.; Zhao,L.
ABSTRACT Prevalence of bovine tuberculosis infection in cattle in Aksu Prefecture determined by intradermal tuberculin skin test (TST), between 1985 and 2016. Cattle were analyzed according to region, feeding pattern, herds and age. A total of 890,009 cattle were tested, with overall bovine tuberculosis prevalence of 0.13% (1172/890009). Statistically significant difference was found in feeding pattern and herds. Prevalence in cows (0.19%, 615/327022) was higher than that in beeves (P&lt; 0.01, OR= 1.903, 95% CI = 1.696 to 2.134). Significant difference (P&lt; 0.01; OR= 2.238, 95%; CI= 1.937 to 2.585) was evident for rates for bovine tuberculosis in the peasant household (0.12%, 942/802343) and farm groups (0.26%, 230/87666). The overall prevalence of bTB...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prevalence; Bovine tuberculosis; Aksu prefecture.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200374

Expression of intronic miRNAs and their host gene Igf2 in a murine unilateral ureteral obstruction model
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Li,N.Q.; Yang,J.; Cui,L.; Ma,N.; Zhang,L.; Hao,L.R.
The objective of this study was to determine the expression of miR-483 and miR-483* and the relationship among them, their host gene (Igf2), and other cytokines in a murine model of renal fibrosis. The extent of renal fibrosis was visualized using Masson staining, and fibrosis was scored 3 days and 1 and 2 weeks after unilateral ureteral obstruction (UUO). Expression of miR-483, miR-483* and various cytokine mRNAs was detected by real-time polymerase chain reaction (PCR). Expression of miR-483 and miR-483* was significantly upregulated in the UUO model, particularly miR-483 expression was the greatest 2 weeks after surgery. Additionally, miR-483 and miR-483* expression negatively correlated with Bmp7 expression and positively correlated with Igf2, Tgfβ,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNA miR-483 Renal fibrosis Igf2; UUO.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600486

Testosterone therapy delays cardiomyocyte aging via an androgen receptor-independent pathway
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,L.; Wu,S.Z.; Ruan,Y.J.; Hong,L.; Xing,X.W.; Lai,W.Y.
The testicular feminized (Tfm) mouse carries a nonfunctional androgen receptor (AR) and reduced circulating testosterone levels. We used Tfm and castrated mice to determine whether testosterone modulates markers of aging in cardiomyocytes via its classic AR-dependent pathway or conversion to estradiol. Male littermates and Tfm mice were divided into 6 experimental groups. Castrated littermates (group 1) and sham-operated Tfm mice (group 2, N = 8 each) received testosterone. Sham-operated Tfm mice received testosterone in combination with the aromatase inhibitor anastrazole (group 3, N = 7). Castrated littermates (group 4) and sham-operated untreated Tfm mice (group 5) were used as controls (N = 8 and 7, respectively). An additional control group (group 6)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Testosterone; Cardiomyocyte; Androgen receptor; Estradiol; Tfm mice.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100007

Bevacizumab and gefitinib enhanced whole-brain radiation therapy for brain metastases due to non-small-cell lung cancer
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Yang,R.F.; Yu,B.; Zhang,R.Q.; Wang,X.H.; Li,C.; Wang,P.; Zhang,Y.; Han,B.; Gao,X.X.; Zhang,L.; Jiang,Z.M.
Non-small-cell lung cancer (NSCLC) patients who experience brain metastases are usually associated with poor prognostic outcomes. This retrospective study proposed to assess whether bevacizumab or gefitinib can be used to improve the effectiveness of whole brain radiotherapy (WBRT) in managing patients with brain metastases. A total of 218 NSCLC patients with multiple brain metastases were retrospectively included in this study and were randomly allocated to bevacizumab-gefitinib-WBRT group (n=76), gefitinib-WBRT group (n=77) and WBRT group (n=75). Then, tumor responses were evaluated every 2 months based on Response Evaluation Criteria in Solid Tumors version 1.0. Karnofsky performance status and neurologic examination were documented every 6 months after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-small-cell lung cancer; Brain metastasis; Bevacizumab; Gefitinib; Whole brain radiotherapy.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100606

Negative emotions affect postoperative scores for evaluating functional knee recovery and quality of life after total knee replacement
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Qi,A.; Lin,C.; Zhou,A.; Du,J.; Jia,X.; Sun,L.; Zhang,G.; Zhang,L.; Liu,M.
This study aimed to determine whether psychological factors affect health-related quality of life (HRQL) and recovery of knee function in total knee replacement (TKR) patients. A total of 119 TKR patients (male: 38; female: 81) completed the Beck Anxiety Inventory (BAI), Beck Depression Inventory (BDI), State Trait Anxiety Inventory (STAI), Eysenck Personality Questionnaire-revised (EPQR-S), Knee Society Score (KSS), and HRQL (SF-36). At 1 and 6 months after surgery, anxiety, depression, and KSS scores in TKR patients were significantly better compared with those preoperatively (P&lt;0.05). SF-36 scores at the sixth month after surgery were significantly improved compared with preoperative scores (P&lt;0.001). Preoperative Physical Component Summary Scale...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Total knee replacement; Negative emotion; Anxiety; Depression; Functional recovery; Health-related quality of life.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100602

Protective roles of pulmonary rehabilitation mixture in experimental pulmonary fibrosis in vitro and in vivo
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,L.; Ji,Y.X.; Jiang,W.L.; Lv,C.J.
Abnormal high mobility group protein B1 (HMGB1) activation is involved in the pathogenesis of pulmonary fibrosis. Pulmonary rehabilitation mixture (PRM), which combines extracts from eight traditional Chinese medicines, has very good lung protection in clinical use. However, it is not known if PRM has anti-fibrotic activity. In this study, we investigated the effects of PRM on transforming growth factor-β1 (TGF-β1)-mediated and bleomycin (BLM)-induced pulmonary fibrosis in vitro and in vivo. The effects of PRM on TGF-β1-mediated epithelial-mesenchymal transition (EMT) in A549 cells, on the proliferation of human lung fibroblasts (HLF-1) in vitro, and on BLM-induced pulmonary fibrosis in vivo were investigated. PRM treatment resulted in a reduction of EMT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High mobility group protein B1; Epithelial-mesenchymal transition; Pulmonary rehabilitation mixture; Receptor for advanced glycation end-product.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600545

Targeting PPM1D by lentivirus-mediated RNA interference inhibits the tumorigenicity of bladder cancer cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Wang,W.; Zhu,H.; Zhang,H.; Zhang,L.; Ding,Q.; Jiang,H.
Protein phosphatase magnesium/manganese-dependent 1D (PPM1D) is a p53-induced phosphatase that functions as a negative regulator of stress response pathways and has oncogenic properties. However, the functional role ofPPM1D in bladder cancer (BC) remains largely unknown. In the present study, lentivirus vectors carrying small hairpin RNA (shRNA) targeting PPM1D were used to explore the effects ofPPM1D knockdown on BC cell proliferation and tumorigenesis. shRNA-mediated knockdown of PPM1D significantly inhibited cell growth and colony forming ability in the BC cell lines 5637 and T24. Flow cytometric analysis showed that PPM1D silencing increased the proportion of cells in the G0/G1 phase. Downregulation of PPM1Dalso inhibited 5637 cell tumorigenicity in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein phosphatase magnesium/manganese-dependent 1D; Bladder cancer; Gene silencing; RNA interference; Proliferation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201044

Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,L.; Ding,Y.; Rao,G.Z.; Miao,D.
The effects of interleukin-10 (IL-10) and glucose on mRNA and protein expression of osteoprotegerin (OPG), and its ligand, receptor activator of nuclear factor-κB ligand (RANKL), were investigated in human periodontal ligament fibroblasts (HPDLFs). Primary HPDLFs were treated with different concentrations of IL-10 (0, 1, 10, 25, 50, and 100 ng/mL) or glucose (0, 5.5, 10, 20, 30, and 40 mmol/L). Changes in mRNA and protein expression were examined using the reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. After IL-10 treatment, mRNA and protein levels of OPG were increased, while mRNA and protein levels of RANKL were decreased (P&lt;0.05), both in a concentration-dependent manner. Glucose stimulation had the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human periodontal ligament fibroblasts; Interleukin-10; Glucose; Osteoprotegerin; Receptor activator of nuclear factor-κB ligand.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400605

Downregulation of survivin by siRNA inhibits invasion and promotes apoptosis in neuroblastoma SH-SY5Y cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,L.; Liang,H.; Cao,W.; Xu,R.; Ju,X.L.
Neuroblastoma is a solid tumor that occurs mainly in children. Malignant neuroblastomas have a poor prognosis because conventional chemotherapeutic agents are not very effective. Survivin, a member of the inhibitor of the apoptosis protein family, plays a significant role in cell division, inhibition of apoptosis, and promotion of cell proliferation and invasion. Previous studies found that survivin is highly expressed in some malignant neuroblastomas and is correlated with poor prognosis. The aim of this study was to investigate whether survivin could serve as a potential therapeutic target of human neuroblastoma. We employed RNA interference to reduce survivin expression in the human neuroblastoma SH-SY5Y cell line and analyzed the effect of RNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuroblastoma; Survivin; Apoptosis; Invasion; RNA interference.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700548

Rankl expression predicts poor prognosis in gastric cancer patients: results from a retrospective and single-center analysis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,X.; Song,Y.; Song,N.; Zhang,L.; Wang,Y.; Li,D.; Wang,Z.; Qu,X.; Liu,Y.
The receptor activator of nuclear factor κB ligand (RANKL)/RANK pathway plays an important role in the prognosis of several solid tumor types, but its role in gastric cancer prognosis has been poorly characterized. A total of 116 gastric cancer patients who underwent surgical resection were enrolled in this study. Expressions of RANKL and RANK in gastric cancer tissues were detected using immunohistochemical staining. Thirty-eight patients (33%) showed a high level of RANKL expression and 61 patients (53%) showed a high level of RANK expression. There was a positive correlation between expressions of RANKL and RANK (P=0.014, r=0.221). A high level of RANKL expression indicated shorter overall survival (OS) (P=0.008), and was associated with a higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RANKL; RANK; Gastric cancer; IHC; Overall survival.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300602

Diabetes mellitus affects the biomechanical function of the callus and the expression of TGF-beta1 and BMP2 in an early stage of fracture healing
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Xu,M.T.; Sun,S.; Zhang,L.; Xu,F.; Du,S.L.; Zhang,X.D.; Wang,D.W.
Transforming growth factor beta 1 (TGF-β1) and bone morphogenetic protein-2 (BMP-2) are important regulators of bone repair and regeneration. In this study, we examined whether TGF-β1 and BMP-2 expressions were delayed during bone healing in type 1 diabetes mellitus. Tibial fractures were created in 95 diabetic and 95 control adult male Wistar rats of 10 weeks of age. At 1, 2, 3, 4, and 5 weeks after fracture induction, five rats were sacrificed from each group. The expressions of TGF-β1 and BMP2 in the fractured tibias were measured by immunohistochemistry and quantitative reverse-transcription polymerase chain reaction, weekly for the first 5 weeks post-fracture. Mechanical parameters (bending rigidity, torsional rigidity, destruction torque) of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; Biomechanical function; TGF-beta1; BMP-2; Fracture healing; Mechanical parameters.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100604

Silencing of augmenter of liver regeneration inhibited cell proliferation and triggered apoptosis in U266 human multiple myeloma cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zeng,H.Q.; Luo,Y.; Lou,S.F.; Liu,Q.; Zhang,L.; Deng,J.C.
Augmenter of liver regeneration (ALR) is a thermostable cytokine that was originally identified to promote the growth of hepatocytes. This study was conducted to explore the expression and function of ALR in multiple myeloma (MM), a common hematologic malignancy. Real-time PCR and western blot analysis were performed to detect the expression of ALR in U266 human MM cells and healthy peripheral blood mononuclear cells (PBMCs). U266 MM cells were exposed to 20 or 40 μg/mL of recombinant ALR and tested for cell proliferation. Small interfering RNA-mediated silencing of ALR was done to investigate the role of ALR in cell proliferation, apoptosis, and cytokine production. Compared to PBMCs, U266 MM cells exhibited significantly higher levels of ALR at both the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Augmenter of liver regeneration; Hematologic cancer; Proliferation; Small interfering RNA.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000611

Intramyocardial implantation of differentiated rat bone marrow mesenchymal stem cells enhanced by TGF-β1 improves cardiac function in heart failure rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Lv,Y.; Liu,B.; Wang,H.P.; Zhang,L.
The present study tested the hypotheses that i) transforming growth factor beta 1 (TGF-β1) enhances differentiation of rat bone marrow mesenchymal stem cells (MSCs) towards the cardiomyogenic phenotype and ii) intramyocardial implantation of the TGF-β1-treated MSCs improves cardiac function in heart failure rats. MSCs were treated with different concentrations of TGF-β1 for 72 h, and then morphological characteristics, surface antigens and mRNA expression of several transcription factors were assessed. Intramyocardial implantation of these TGF-β1-treated MSCs to infarcted heart was also investigated. MSCs were initially spindle-shaped with irregular processes. On day 28 after TGF-β1 treatment, MSCs showed fusiform shape, orientating parallel with one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow mesenchymal stem cell; Transforming growth factor beta1; Cardiomyocytes; Differentiation; Intramyocardial implantation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600603

Comparative efficacy and safety of contact force-sensing catheter and second-generation cryoballoon ablation for atrial fibrillation: a meta-analysis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhou,X.; Lv,W.; Zhang,W.; Ye,Y.; Li,Y.; Zhou,Q.; Zhang,J.; Xing,Q.; Lu,Y.; Zhang,L.; Wang,H.; Qin,W.; Tang,B.
This meta-analysis compared the efficacy and safety of the contact force (CF)-sensing catheter and second-generation cryoballoon (CB) ablation for treating atrial fibrillation (AF). Six controlled clinical trials comparing ablation for AF using a CF-sensing catheter or second-generation CB were identified from PubMed, EMBASE, Cochrane Library, Wanfang Data, and China National Knowledge Infrastructure. The procedure duration was significantly lower in the CB group compared with that in the CF group [mean difference (MD)=29.4; 95%CI=17.84–40.96; P=0.01], whereas there was no difference between the groups for fluoroscopy duration (MD=0.59; 95%CI=–4.48–5.66; P=0.82). Moreover, there was no difference in the incidence of non-lethal complications (embolic event,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atrial fibrillation; Ablation; Contact force-sensing catheter; Second-generation cryoballoon; Meta-analysis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900607