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Isolation of high quality RNA from Polyporus umbellatus (Pers.) Fries
Provedor de dados: Electronic Journal of Biotechnology
Autores: Zhang,Yuejin; Qin,Yali; Guo,Lijun; Zhou,Zili; Liang,Zongsuo; Zhang,Chenlu; Guo,Hongbo.
Background: The dried sclerotium of medicinal fungus Polyporus umbellatus (Pers.) Fries has many pharmacological functions such as diuretic and anticancer activity, in which high-content polysaccharides may play an important role. However, RNA isolation is difficult in filamentous fungi and lacking in P. umbellatus. Results: Five methods for RNA extraction from five strains collected from four provinces were assessed for their ability to recover a high-quality RNA applicable for sequence-related amplification polymorphism (SRAP) PCR and GDP-D-mannose pyrophosphorylase (GMP) gene expression profiles. Both A260/A280 and A260/A230 ratios of the best Trizol Plus + RNAiso-mate for Plant Tissue method are around 2 with a yield of 1122.00 ± 0.21 ng &amp;#956;l-1....
Tipo: Journal article
Palavras-chave: Chuling; Medicinal fungus; Polyporus umbellatus; RNA isolation.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500010