Consulta: creatorFacets:"Zucchi,Maria Imaculada"
Registros recuperados: 23
Data/hora: 04/06/2024 06:26:10

Origin, evolution and genome distribution of microsatellites
Provedor de dados: 74
Autores: Oliveira,Eder Jorge; Pádua,Juliano Gomes; Zucchi,Maria Imaculada; Vencovsky,Roland; Vieira,Maria Lúcia Carneiro.
Microsatellites, or simple sequence repeats (SSRs), have been the most widely applied class of molecular markers used in genetic studies, with applications in many fields of genetics including genetic conservation, population genetics, molecular breeding, and paternity testing. This range of applications is due to the fact that microsatellite markers are co-dominant and multi-allelic, are highly reproducible, have high-resolution and are based on the polymerase chain reaction (PCR). When first introduced, the development of microsatellite markers was expensive but now new and efficient methods of repetitive sequence isolation have been reported, which have led to reduced costs and microsatellite-technology has been increasingly applied to several species,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Molecular genetics; Genetic structure of populations.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200018

Genetic divergence of rubber tree estimated by multivariate techniques and microsatellite markers
Provedor de dados: 74
Autores: Gouvêa,Lígia Regina Lima; Rubiano,Luciana Benchimol; Chioratto,Alisson Fernando; Zucchi,Maria Imaculada; Gonçalves,Paulo de Souza.
Genetic diversity of 60 Hevea genotypes, consisting of Asiatic, Amazonian, African and IAC clones, and pertaining to the genetic breeding program of the Agronomic Institute (IAC), Brazil, was estimated. Analyses were based on phenotypic multivariate parameters and microsatellites. Five agronomic descriptors were employed in multivariate procedures, such as Standard Euclidian Distance, Tocher clustering and principal component analysis. Genetic variability among the genotypes was estimated with 68 selected polymorphic SSRs, by way of Modified Rogers Genetic Distance and UPGMA clustering. Structure software in a Bayesian approach was used in discriminating among groups. Genetic diversity was estimated through Nei's statistics. The genotypes were clustered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genealogy; Genetic diversity; Hevea brasiliensis; Multivariate analysis; SSRs.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200018

The genetic structure and mating system of Acrocomia aculeata (Arecaceae)
Provedor de dados: 74
Autores: Abreu,Aluana Gonçalves; Priolli,Regina Helena Geribello; Azevedo-Filho,Joaquim Adelino; Nucci,Stella Maris; Zucchi,Maria Imaculada; Coelho,Ricardo Marques; Colombo,Carlos Augusto.
Acrocomia aculeata is a perennial, fruit-producing palm tree, native to tropical forests. Its fruits have spurred interest because of their significant potential for use in the cosmetic industry and as feedstock for biofuel. In the present study, the genetic structure and mating system in Acrocomia aculeata were analyzed, using eight nuclear microsatellite loci and samples from São Paulo and Minas Gerais states, Brazil. By means of Bayesian analysis, these populations were clustered into two or three groups. A high multilocus outcrossing rate suggests that outcrosses were predominant, although a certain degree of biparental inbreeding also occurred. Thus, although monoecious and self-compatible, there is every indication that A. aculeata bears a mixed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; Mating system.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100016

Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers
Provedor de dados: 74
Autores: Zucchi,Maria Imaculada; Brondani,Rosana Pereira Vianello; Pinheiro,José Baldin; Chaves,Lázaro José; Coelho,Alexandre Siqueira Guedes; Vencovsky,Roland.
The ''cagaita tree'' (Eugenia dysenterica) is a plant found widespread in the Brazilian Cerrado. Its fruit is used for popular consumption and for industrial purposes. This study opens a new perspective for the generation of population genetic data and parameters estimates for devising sound collection and conservation procedures for Eugenia dysenterica. A battery of 356 primer pairs developed for Eucalyptus spp. was tested on the ''cagaita tree''. Only 10 primer pairs were found to be transferable between the two species. Using a polyacrilamide gel, an average of 10.4 alleles per locus was detected, in a sample of 116 individuals from 10 natural ''cagaita tree'' populations. Seven polymorphic loci allowed estimation of genetic parameters, including...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; Myrtaceae; Cerrado; Tropical tree; Genetic diversity and transferability.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400008

Genetic structure of Brazilian wild rice (Oryza glumaepatula Steud., Poaceae) populations analyzed using microsatellite markers
Provedor de dados: 74
Autores: Karasawa,Marines M.G.; Vencovsky,Roland; Silva,Cynthia M.; Zucchi,Maria Imaculada; Oliveira,Giancarlo C.X.; Veasey,Elizabeth A.
Knowledge of the genetic structure and diversity of natural populations is important in developing strategies for in situ and ex situ conservation. We used eight microsatellite loci to estimate genetic structure and investigate within and between population genetic variation in eleven Brazilian wild rice (Oryza glumaepatula) populations. The study showed the following genetic diversity parameters: average number of 3.1 alleles per locus; 77.3% polymorphic loci; 0.091 observed heterozygosity and 0.393 gene diversity. F-statistics detected by microsatellite loci were: F ST = 0.491 (and R ST = 0.608), F IS = 0.780 and F IT = 0.888. No population was in Hardy-Weinberg equilibrium. The estimated apparent outcrossing rate (0.143) indicated a predominance of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Gene flow; Genetic diversity; Microsatellites; Oryza glumaepatula.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300017

Using genetic diversity information to establish core collections of Stylosanthes capitata and Stylosanthes macrocephala
Provedor de dados: 74
Autores: Santos-Garcia,Melissa Oliveira; Toledo-Silva,Guilherme de; Sassaki,Rodrigo Possidonio; Ferreira,Thais Helena; Resende,Rosângela Maria Simeão; Chiari,Lucimara; Karia,Cláudio Takao; Carvalho,Marcelo Ayres; Faleiro,Fábio Gelape; Zucchi,Maria Imaculada; Souza,Anete Pereira de.
Stylosanthes species are important forage legumes in tropical and subtropical areas. S. macrocephala and S. capitata germplasm collections that consist of 134 and 192 accessions, respectively, are maintained at the Brazilian Agricultural Research Corporation Cerrados (Embrapa-Cerrados). Polymorphic microsatellite markers were used to assess genetic diversity and population structure with the aim to assemble a core collection. The mean values of H O and H E for S. macrocephala were 0.08 and 0.36, respectively, whereas the means for S. capitata were 0.48 and 0.50, respectively. Roger's genetic distance varied from 0 to 0.83 for S. macrocephala and from 0 to 0.85 for S. capitata. Analysis with STRUCTURE software distinguished five groups among the S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stylosanthes; Tropical forage; Microsatellites; Genetic diversity; Core collection.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500020

Caffeine inheritance in interspecific hybrids of Coffea arabica x Coffea canephora (Gentianales, Rubiaceae)
Provedor de dados: 74
Autores: Priolli,Regina H.G.; Mazzafera,Paulo; Siqueira,Walter J.; Möller,Milene; Zucchi,Maria Imaculada; Ramos,Luis Carlos S.; Gallo,Paulo B.; Colombo,Carlos A.
Caffeine inheritance was investigated in F2 and BC1F1 generations between Coffea arabica var. Bourbon Vermelho (BV) and Coffea canephora var. Robusta 4x (R4x). The caffeine content of seeds and leaves was determined during 2004 and 2005. Microsatellite loci-markers were used to deduce the meiotic pattern of chromosome pairing of tetraploid interspecific hybrids. Genetic analysis indicated that caffeine content in seeds was quantitatively inherited and controlled by genes with additive effects. The estimates of broad-sense heritability of caffeine content in seeds were high for both generations. In coffee leaves, the caffeine content (BSH) from the same populations showed transgressive segregants with enhanced levels and high BSH. Segregation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broad sense heritability; Coffee breeding; Microsatellite-markers; Quantitative genes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300017

Genetic diversity in cultivated carioca common beans based on molecular marker analysis
Provedor de dados: 74
Autores: Perseguini,Juliana Morini Küpper Cardoso; Chioratto,Alisson Fernando; Zucchi,Maria Imaculada; Colombo,Carlos Augusto; Carbonell,Sérgio Augusto Moraes; Mondego,Jorge Mauricio Costa; Gazaffi,Rodrigo; Garcia,Antonio Augusto Franco; Campos,Tatiana de; Souza,Anete Pereira de; Rubiano,Luciana Benchimol.
A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger's modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLPs; Genetic structure; Genetic variability; Phaseolus vulgaris L.; SSRs.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100017

Genetic instability of sugarcane plants derived from meristem cultures
Provedor de dados: 74
Autores: Zucchi,Maria Imaculada; Arizono,Hideto; Morais,Vicente Alberto; Fungaro,Maria Helena Pelegrinelli; Vieira,Maria Lúcia Carneiro.
The RADP (Random amplified polymorphic DNA) technique was used to detect tissue-culture-induced variations in sugarcane. Plants of the Brazilian variety RB83-5486 propagated via rhizomes and via meristem cultures were studied. The polymorphism rate for 98 RAPD loci was 6.93% when the plants derived from meristems. Besides, in order to evaluate the influence of the number of subcultures on the generation of somaclonal variation, field-grown RB83-5486 plants derived from 10 meristems were studied after five subcultivations. Although different rates of polymorphism were observed, there was no direct association with the stage of subcultivation. The analysis of plants of two sugarcane varieties cultivated in vitro from meristems showed that variety RB83-5486...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sugarcane; Somaclonal variation; Meristem cultures; Phenotypic instability; RAPD.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100017

Developing a common bean core collection suitable for association mapping studies
Provedor de dados: 74
Autores: Perseguini,Juliana Morini Küpper Cardoso; Silva,Gliciane Micaele Borges; Rosa,João Ricardo Bachega Feijó; Gazaffi,Rodrigo; Marçal,Jéssica Fernanda; Carbonell,Sérgio Augusto Morais; Chiorato,Alisson Fernando; Zucchi,Maria Imaculada; Garcia,Antonio Augusto Franco; Benchimol-Reis,Luciana Lasry.
Because of the continuous introduction of germplasm from abroad, some collections have a high number of accessions, making it difficult to explore the genetic variability present in a germplasm bank for conservation and breeding purposes. Therefore, the aim of this study was to quantify and analyze the structure of genetic variability among 500 common bean accessions to construct a core collection. A total of 58 SSRs were used for this purpose. The polymorphism information content (PIC) in the 180 common bean accessions selected to compose the core collection ranged from 0.17 to 0.86, and the discriminatory power (DP) ranged from 0.21 to 0.90. The 500 accessions were clustered into 15 distinct groups and the 180 accessions into four distinct groups in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Genetic diversity; Genetic structure; Microsatellites; Phaseolus vulgaris L.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100067

High genetic diversity among and within bitter manioc varieties cultivated in different soil types in Central Amazonia
Provedor de dados: 74
Autores: Alves-Pereira,Alessandro; Peroni,Nivaldo; Cavallari,Marcelo Mattos; Lemes,Maristerra R.; Zucchi,Maria Imaculada; Clement,Charles R.
Abstract Although manioc is well adapted to nutrient-poor Oxisols of Amazonia, ethnobotanical observations show that bitter manioc is also frequently cultivated in the highly fertile soils of the floodplains and Amazonian dark earths (ADE) along the middle Madeira River. Because different sets of varieties are grown in each soil type, and there are agronomic similarities between ADE and floodplain varieties, it was hypothesized that varieties grown in ADE and floodplain were more closely related to each other than either is to varieties grown in Oxisols. We tested this hypothesis evaluating the intra-varietal genetic diversity and the genetic relationships among manioc varieties commonly cultivated in Oxisols, ADE and floodplain soils. Genetic results did...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Microsatellites; Amazonian dark earths; Floodplain; Oxisols.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300468

Variabilidade genética do umbuzeiro no Semi-Árido brasileiro, por meio de marcadores AFLP
Provedor de dados: 86
Autores: Santos,Carlos Antonio Fernandes; Rodrigues,Marciene Amorim; Zucchi,Maria Imaculada.
O objetivo deste trabalho foi avaliar a distribuição da variabilidade genética do umbuzeiro (Spondias tuberosa), no Semi-Árido brasileiro, por meio de marcadores AFLP, para subsidiar estratégias de prospecção e conservação da espécie. Foram analisados 68 indivíduos de umbuzeiro de 15 ecorregiões, pelo dendrograma UPGMA e pela dispersão em escala multidimensional (MDS), com o coeficiente de Jaccard de 141 bandas polimórficas de AFLP. A análise da variância molecular foi realizada pela decomposição total entre e dentro das regiões ecogeográficas. O dendrograma apresentou valor cofenético de 0,96, e o gráfico MDS apresentou 0,25 para a falta de ajustamento. A variabilidade genética do umbuzeiro foi estimada em 0,3138, o que indica grande variação entre os...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spondias tuberosa; Análise multivariada; Análise de variância molecular; Dendrograma; Escala multidimensional.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000800013

Genetic structure and gene flow of Eugenia dysenterica natural populations
Provedor de dados: 86
Autores: Zucchi,Maria Imaculada; Pinheiro,José Baldin; Chaves,Lázaro José; Coelho,Alexandre Siqueira Guedes; Couto,Mansuêmia Alves; Morais,Lizz Kezzy de; Vencovsky,Roland.
This study was carried out to assess the genetic variability of ten "cagaita" tree (Eugenia dysenterica) populations in Southeastern Goiás. Fifty-four randomly amplified polymorphic DNA (RAPD) loci were used to characterize the population genetic variability, using the analysis of molecular variance (AMOVA). A phiST value of 0.2703 was obtained, showing that 27.03% and 72.97% of the genetic variability is present among and within populations, respectively. The Pearson correlation coefficient (r) among the genetic distances matrix (1 - Jaccard similarity index) and the geographic distances were estimated, and a strong positive correlation was detected. Results suggest that these populations are differentiating through a stochastic process, with restricted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myrtaceae; Cerrado; Tropical species; Genetic diversity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005001000005

Similaridade genética entre raças bovinas brasileiras
Provedor de dados: 86
Autores: Rangel,Priscila Nascimento; Zucchi,Maria Imaculada; Ferreira,Márcio Elias.
A similaridade genética entre animais de duas raças bovinas brasileiras (Crioulo Lageano e Junqueira) foi estimada pela análise de polimorfismo de DNA amplificado ao acaso (RAPD), tendo como referência (outgroups) animais de raças comerciais das espécies Bos taurus e B. indicus. Estas duas raças possuem grande similaridade fenotípica, sugerindo uma origem genética comum. Uma matriz de similaridade genética baseada em polimorfismo de DNA foi obtida e representada graficamente por um dendrograma, definido após processo estatístico de reamostragem bootstrap. Ao contrário do que era previsto com base nas semelhanças morfológicas das duas raças, os animais das raças Crioulo Lageano e Junqueira não apresentaram similaridade elevada entre si quando comparados com...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Bovino; Marcador molecular; Distância genética; RAPD.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000100015

Genetic diversity in soybean germplasm identified by SSR and EST-SSR markers
Provedor de dados: 86
Autores: Mulato,Bruno Mello; Möller,Milene; Zucchi,Maria Imaculada; Quecini,Vera; Pinheiro,José Baldin.
The objectives of this work were to investigate the genetic variation in 79 soybean (Glycine max) accessions from different regions of the world, to cluster the accessions based on their similarity, and to test the correlation between the two types of markers used. Simple sequence repeat markers present in genomic (SSR) and in expressed regions (EST-SSR) were used. Thirty SSR primer-pairs were selected (20 genomic and 10 EST-SSR) based on their distribution on the 20 genetic linkage groups of soybean, on their trinucleotide repetition unit and on their polymorphism information content. All analyzed loci were polymorphic, and 259 alleles were found. The number of alleles per locus varied from 2-21, with an average of 8.63. The accessions exhibit a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Molecular marker; Plant breeding; Plant germplasm; Polymorphism information content.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000300007

Relação entre a variação genética de caracteres quantitativos e marcadores moleculares em subpopulações de cagaiteira (Eugenia dysenterica DC)
Provedor de dados: 94
Autores: Aguiar,Ananda Virginia de; Moura,Nara Fernandes; Moura,Mara Fernandes; Zucchi,Maria Imaculada; Vencovsky,Roland; Chaves,Lázaro José.
O objetivo do trabalho foi relacionar a diversidade genética medida a partir de três diferentes marcadores moleculares, com a variação genética quantitativa de caracteres poligênicos, estimada em ensaio de progênies, sob condições controladas. As progênies, oriundas de dez subpopulações naturais de cagaiteira do sudeste de Goiás, foram avaliadas em experimento em blocos completos casualizados, com quatro repetições e uma planta por parcela. Foram estimadas a herdabilidade ao nível de média de progênies (h mi ²) e o coeficiente de variação genética (CVgi) de cada subpopulação para a altura da planta e o diâmetro do fuste, por quatro anos, bem como para as respectivas taxas de crescimento. Estimativas da diversidade gênica (Hei) e do índice de fixação (f i)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Correlação; Regressão múltipla; Marcadores moleculares; Caracteres quantitativos; Conservação genética.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452011000100021

A note on the distribution of genetic diversity of Anglo-Nubian goats in central-northern farms of Piauí, Brazil
Provedor de dados: 96
Autores: Silvestre,Ellida de Aguiar; Costa,Márcio da Silva; Silva,Pollyana Oliveira da; Bajay,Miklos Maximiliano; Pinheiro,José Baldin; Zucchi,Maria Imaculada; Campelo,José Elivalto Guimarães; Britto,Fábio Barros.
The objective of this study was to characterize the genetic variability of Anglo-Nubian goats using microsatellite markers. The study was conducted using herds from four municipalities of Central-Northern Piauí (Teresina, José de Freitas, Campo Maior, and Angical), where technical information is scarce. Seven markers suggested by FAO were used (ILSTS11, McM527, INRA23, ETH10, OarfCB304, OarfCB48, and MAF209). The samples were genotyped using a 7% polyacrylamide gel. The average number of alleles per locus was 4.0, with observed and expected heterozygosity of 0.38 and 0.55, respectively. Few deviations from the Hardy-Weinberg equilibrium were observed for each population. Only two loci deviated significantly in two localities. The coefficient of gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endogamy; Genetic structure; Microsatellite; Molecular markers.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015000400155

Caracterização genética de mulungu (Erythrina velutina Willd.) em áreas de baixa ocorrência
Provedor de dados: 124
Autores: Gonçalves,Luciana Oliveira; Pinheiro,José Baldin; Zucchi,Maria Imaculada; Silva-Mann,Renata.
A Erythrina velutina Willd., popularmente conhecida como mulungu, apresenta propriedades farmacológicas e se encontra em estado de raridade em regiões do Estado de Sergipe. Devido ao interesse econômico, torna-se fundamental gerar informações para definir planos de conservação e exploração sustentável. Este trabalho teve como objetivo caracterizar os indivíduos de Erythrina velutina por meio de marcadores ISSRs. Para isto foram estudados 40 indivíduos do Estado de Sergipe pertencentes a três localidades (Município de Pinhão - Caatinga; Município de Santana do São Francisco e São Cristóvão - Mata Atlântica). Ao total, foram avaliados e obtidos 149 locos a partir de 11 primers ISSR. O número mínimo de locos para estudo de diversidade em E. velutina foi de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genética vegetal; Mulungu.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000200009

Morphological variation and isozyme diversity in Dioscorea alata L. landraces from Vale do Ribeira, Brazil
Provedor de dados: 63
Autores: Bressan,Eduardo de Andrade; Briner Neto,Thiago; Zucchi,Maria Imaculada; Rabello,Ronaldo José; Veasey,Elizabeth Ann.
Traditional growers of the Vale do Ribeira, São Paulo State, grow and make use of several D. alata landraces. This study assessed the genetic diversity of 16 landraces, using isozymatic and morphological markers and comparing them with 19 commercial varieties of D. alata. Their distribution in different levels of organization such as households and communities of the Vale do Ribeira was evaluated. Isozymatic analyses were performed with polyacrylamide (six systems) and starch gels (one system), while the morphological analyses were carried out with 24 traits. Due to the polyploid nature of this species, the isozymatic bands were scored as binary data. Morphological traits were also scored as binary data. Principal coordinates and cluster analyses were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AMOVA; Traditional agriculture; Genetic diversity; Water yam.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400016

A lima bean core collection based on molecular markers
Provedor de dados: 63
Autores: Gomes,Regina Lucia Ferreira; Costa,Marcones Ferreira; Alves-Pereira,Alessandro; Bajay,Miklos Maximiliano; Viana,João Paulo Gomes; Valente,Sérgio Emílio dos Santos; Lopes,Ângela Celis de Almeida; Zucchi,Maria Imaculada; Pinheiro,José Baldin.
ABSTRACT Some germplasm collections have a high number of accessions, which makes it difficult to explore the genetic variability present in the germplasm bank due to the redundancy and the difficulty of detailed analysis of all conserved accessions. Therefore, our study aimed to analyze the genetic diversity of 153 lima bean (Phaseolus lunatus) accessions for the purpose of constructing a core collection. Eleven SSRs were used for this purpose. The 153 lima bean accessions can be represented by low redundancy using a minimum of 34 accessions, thus representing 22 % of the size of the entire germplasm bank. The core collection had a higher Shannon diversity index and expected heterozygosity (1.906 and 0.811, respectively) than those presented by the entire...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Germplasm; Genetic diversity; Genetic structure; Microsatellite markers.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000201100

Genetic diversity of American wild rice species
Provedor de dados: 63
Autores: Veasey,Elizabeth Ann; Bressan,Eduardo de Andrade; Zucchi,Maria Imaculada; Vencovsky,Roland; Cardim,Daruska Cavalcante; Silva,Rainério Meireles da.
Studies on genetic diversity and genetic structure of natural populations are important in order to define strategies for in situ and ex situ conservation actions and for plant pre-breeding programs. Aiming to assess the genetic diversity and genetic structure of three wild American Oryza species with isozyme markers, 14 populations of the diploid O. glumaepatula (AglAgl), 11 populations of the tetraploid O. grandiglumis (CCDD) and five populations of the also tetraploid O. latifolia (CCDD) were studied. They were all originated from Rio Paraguay hydrographic basin and the Amazon. Four enzymes were used and they gave 40 polymorphic bands. The most polymorphic species was O. glumaepatula, followed by O. latifolia and O. grandiglumis. A cluster analysis with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oryza glumaepatula; O. latifolia; O. grandiglumis; Amazon; Genetic structure.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400008

Genetic diversity analysis among pigeonpea genotypes adapted to South American regions based on microsatellite markers
Provedor de dados: 63
Autores: Sousa,Adna Cristina Barbosa de; Godoy,Rodolfo; Sforça,Danilo Augusto; Campos,Tatiana de; Zucchi,Maria Imaculada; Jank,Liana; Souza,Anete Pereira de.
The pigeonpea [Cajanus cajan (L) Millspaugh] is one of the most important perennial legume crops utilized in the food, fodder, soil conservation, crop-livestock integrated systems, reclaiming of degraded pastures and symbiotic nitrogen fixation. Microsatellite markers were used to estimate the genetic diversity of 77 pigeonpea genotypes selected from the germplasm collections at Embrapa Cattle-Southeast and, to evaluate their transferability to Phaseolus vulgaris and Vigna unguiculata species. The number of alleles per locus ranged from 2 to12, with an average of 5.1 alleles. The PIC values ranged from 0.11 to 0.80 (average 0.49) and the D values from 0.23 to 0.91 (average 0.58). The averages of observed and expected heterozygosity were 0.25 and 0.47,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Legumes; Transferability; Microsatellite; Germplasm.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400007

Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree data
Provedor de dados: 52
Autores: Priolli,Regina Helena Geribello; Pinheiro,José Baldin; Zucchi,Maria Imaculada; Bajay,Miklos Maximiliano; Vello,Natal Antonio.
In this study, simple sequence repeats (SSR) loci and pedigree data were used to investigate the genetic relationship in a group of 168 Brazilian soybean cultivars. Eighteen SSR loci produced an average of 5.06 alleles and a mean gene diversity of 0.58 for the cultivars studied. Genetic distance (GD) was determined using the modified Roger's Wright distance, and a final dendrogram was in agreement with the cultivar pedigree. A distance matrix based on the coefficient of parentage scores was also generated for the cultivars, which ranged from 0 to 1, with a mean of 0.18, whereas SSR-based genetic similarity (1- GD) ranged from 0.01 to 0.90, with a mean of 0.25. Mantel's Z test showed that the similarity matrices generated from both the data sets were low,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coefficient of parentage; Glycine max (L.) Merr.; Microsatellite markers.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300004