Consulta: repository:"47"
Registros recuperados: 240
Data/hora: 07/06/2024 16:07:20

Histological analysis of pollen-pistil interactions in sour passion fruit plants (Passiflora edulis Sims)
Provedor de dados: Biocell
Autores: Madureira,Hérika Chagas; Pereira,Telma Nair Santana; Cunha,Maura Da; Klein,Denise Espellet.
The success of sexual plant reproduction is directly influenced by specific interactions between the pollen and pistil. Light, fluorescence and scanning electron microscopy techniques were used to evaluate the steps of pollination in sour passion fruit plants (Passiflora edulis Sims). In the compatible interaction, pollen tubes grow through stigma projections towards the ovary. The pollen grain surface was found to be spheroidal and to consist of heteroreticulate exine with six colpi. Furthermore, analysis in vivo of pollen-pistil interactions indicated that stigmas of flowers 24 hours before anthesis are unable to discriminate compatible (genetically unrelated) and incompatible (genetically related) pollen grains. Taken together, these results provide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flower; Pollen tube; Pollination; Stigma.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000200005

Development beyond the gastrula stage and digestive organogenesis in the apple-snail Pomacea canaliculata (Architaenioglossa, Ampullariidae)
Provedor de dados: Biocell
Autores: Koch,E.; Winik,B.C.; Castro-Vazquez,A.
Development of Pomacea canaliculata from the gastrula stage until the first day after hatching is described. Trochophore embryos are developed after gastrulation, showing the prototroch as a crown of ciliated orange-brownish cells. However, no true veliger embryos are formed, since the prototroch does not fully develop into a velum. Afterward, the connection between the fore- and midgut is permeated and the midgut becomes full of the pink-reddish albumen, which is stored into a central archenteron's lake, from where it is accumulated into the large cells forming the midgut wall ("giant cells"). Electron microscopy of giant cells in late embryos showed that albumen is engulfed by large endocytic vesicles formed between the irregular microvilli at the top of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alimentary canal; Midgut gland; Symbiosis; Albumen.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000100006

Genomic cloning and characterization of a PPA gene encoding a mannose-binding lectin from Pinellia pedatisecta
Provedor de dados: Biocell
Autores: Lin,Juan; Zhou,Xuanwei; Fei,Jiong; Liao,Zhihua; Jin,Wang; Sun,Xiaofen; Tang,Kexuan.
A gene encoding a mannose-binding lectin, Pinellia pedatisecta agglutinin (PPA), was isolated from leaves of Pinellia pedatisecta using genomic walker technology. The ppa contained an 1140-bp 5’-upstream region, a 771-bp open reading frame (ORF) and an 829-bp 3’-downstream region. The ORF encoded a precursor polypeptide of 256 amino acid residues with a 24-amino acid signal peptide. There were one putative TATA box and six possible CAAT boxes lying in the 5’-upstream region of ppa. The ppa showed significant similarity at the nucleic acid level with genes encoding mannose-binding lectins from other Araceae species such as Pinellia ternata, Arisaema heterophyllum, Colocasia esculenta and Arum maculatum. At the amino acid level, PPA also shared varying...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genomic cloning; Genomic walker technology; Mannose-binding lectin; Pinellia pedatisecta; Pinellia pedatisecta agglutinin (PPA).
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100003

Meta-analysis of the cell cycle related C12orf48
Provedor de dados: Biocell
Autores: Varisli,Lokman.
The cell cycle is a conserved process from yeast to mammals and focuses on mechanisms that regulate the timing and frequency of DNA replication and cell division. The temporal and spatial expression of the genes is tightly regulated to ensure accurate replication and transmission of DNA to daughter cells during the cycle. Although the genes involved in interphase are well studied, most of the genes which are involved in mitotic events still remain unidentified. Since, the discovery of mitosis related genes is still incomplete, we performed a co-expression and gene ontology analysis for revealing novel mitosis regulated genes. In this study, we showed that C12orf48 is co-expressed with well-known mitotic genes. Moreover, it is also co-expressed with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C12orf48; Cell cycle; Co-expression; Gene ontology; Oncomine.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000100002

Abstracts from the Eleventh Multidisciplinary Workshop: (Décimo Primera Jornada Multidisciplinaria) December, 2009 Buenos Aires, Argentina
Provedor de dados: Biocell
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000200006

Correlative microscopy of Purkinje cells
Provedor de dados: Biocell
Autores: Castejón,Orlando J.
The Purkinje cell and their synaptic contacts have been described using (1) light microsocopy, (2) transmission and scanning electron microscopy, and freeze etching technique, (3) conventional and field emission scanning electron microscopy and cryofracture methods, (4) confocal laser scanning microscopy using intravital stain FM64, and (5) immunocytochemical techniques for Synapsin-I, PSD9-5, GluR1 subunit of AMPA receptors, N-cadherin, and CamKII alpha. The outer surface and inner content of plasma membrane, cell organelles, cytoskeleton, nucleus, dendritic and axonal processes have been exposed and analyzed in a three-dimensional view. The intramembrane morphology, in bi- and three-dimensional views, and immunocytochemical labeling of synaptic contacts...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebellar cells; Synaptic contacts; Electron microscopy; Immunohistochemistry.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000100001

Evaluation of cytotoxic potential of latex of Calotropis procera and Podophyllotoxin in Allum cepa root model
Provedor de dados: Biocell
Autores: Sehgal,R.; Roy,S.; Kumar,V.L.
In the present study we have utilized the Allium cepa root tip meristem model to evaluate the cytotoxic and anti-mitotic activities of latex of Calotropis procera (DL) and podophyllotoxin. Standard cytotoxic drug cyclophosphamide and non-cytotoxic drugs cyproheptadine and aspirin served as controls. Like cyclophosphamide, both DL and podophyllotoxin significantly inhibited the growth of roots and mitotic activity in a dose-dependent manner. However, podophyllotoxin was more potent in this regard and produced root decay. Cyproheptadine and aspirin, on the other hand, showed a marginal effect on the root growth and mitotic activity at much higher concentrations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calotropis procera; Podophyllotoxin; Cyclophosphamide; Cytotoxic; Allium cepa.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100002

Germinating seeds of the mung bean, Vigna radiata (Fabaceae), as a model for the preliminary evaluation of cytotoxic effects of drugs
Provedor de dados: Biocell
Autores: Kumar,Vijay L.; Singhal,Abhishek.
Cytotoxic properties of plant extracts and drugs being developed for cancer treatment are usually evaluated by a variety of in vivo and in vitro tests carried out in animal or plant based models. In the present study we have evaluated the possibility of using the germinating mung beans (Vigna radiata), for rapid and inexpensive screening of drugs exhibiting cytotoxic properties. Mung beans were allowed to germinate either in tap water or in different drug solutions, and parameters like percent germination, increase in radicle length, change in seedling weight and mitotic index of apical root meristems were determined at two time intervals coinciding with the time at which the radicle length in control group was 1.0 to 1.5 cm (time 0, T0) and 48 h later...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus aureus; Calotropis procera; Podophyllotoxin; Cyclophosphamide; Aspirin.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000100002

Seed weight variation of wyoming sagebrush in Northern Nevada
Provedor de dados: Biocell
Autores: Busso,Carlos A.; Perryman,Barry L.
Seed size is a crucial plant trait that may potentially affect not only immediate seedling success but also the subsequent generation. We examined variation in seed weight of Wyoming sagebrush (Artemisia tridentata ssp. wyomingensis Beetle and Young), an excellent candidate species for rangeland restoration. The working hypothesis was that a major fraction of spatial and temporal variability in seed size (weight) of Wyoming sagebrush could be explained by variations in mean monthly temperatures and precipitation. Seed collection was conducted at Battle Mountain and Eden Valley sites in northern Nevada, USA, during November of 2002 and 2003. Frequency distributions of seed weight varied from leptokurtic to platykurtic, and from symmetry to skewness to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artemisia tridentata ssp. wyomingensis; Climate effects; Rangeland restoration; Seed size.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300005

Sperm ultrastructure and spermatogenesis in the lizard, Tropidurus itambere
Provedor de dados: Biocell
Autores: Ferreira,Adelina; Dolder,Heidi.
Spermatogenesis, with emphasis on spermiogenesis, is described for the lizard, Tropidurus itambere, using light microscopy, phase contrast and epifluorescence, as well as scanning and transmission electron microscopy. Cellular differentiation involves events of chromatin condensation, nuclear elongation and the formation of structural complexes, such as the acrosomal and axonemal ones. Other new characteristics, exclusive for this species, include various aspects of the subacrosomal granule, the insertion of the pro-acrosomal vesicle and the development of these structures to participate in the acrosomal complex. Radial projections occur just above the nuclear shoulders, which have been recognized already from the beginning of cellular elongation. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ultrastructure; Sperm; Spermatogenesis; Lizard; Tropiduridae.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300006

The recreational fishery in cabra corral reservoir (Argentina): A first comprehensive analysis
Provedor de dados: Biocell
Autores: Baigún,C.; Bernal,R.; Barrientos,D.; Muñoz,L.; Barros,E.; Sauad,J.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pejerrey; Cabra Corral reservoir; Catamarans; Recreational fishery.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100016

Changes in the corpora allata and epidermal proliferation along the fourth instar of the Chagas disease vector Triatoma infestans
Provedor de dados: Biocell
Autores: Ronderos,Jorge R.
Triatoma infestans, a blood-feeding insect, synchronises physiological mechanisms leading to moult with food intake. Since the corpora allata are important in moult and metamorphosis regulation, we have studied morphological changes in 4th instar nymphs (gland size, cell density, percent of animals showing mitoses and cell size). Changes were correlated with the effect of precocene II, epidermal proliferation, and with the extent of the "head critical period". Based on morphological grounds, three stages can be defined in the gland along the 4th instar: Stage 1 (days 0-2 after feeding) showed small corpora allata, composed by a small number of cells, and in which mitoses were absent; Stage 2 (days 3-9) showed growing corpora allata, in which cell number...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Moult; Neuroendocrine control; Juvenile hormones; Precocene II.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000300003

A study of chlorophyll-like and phycobilin pigments in the C endosymbiont of the apple- snail Pomacea canaliculata
Provedor de dados: Biocell
Autores: Vega,Israel A.; Dellagnola,Federico A.; Hurst,Jorge A.; Godoy,Martín S.; Castro-Vazquez,Alfredo.
Pigments present in the brown-greenish C morph of an intracellular endosymbiont of Pomacea canaliculata were investigated. Acetone extracts of the endosymbiotic corpuscles showed an absorption spectrum similar to that of chlorophylls. Three fractions obtained from silica gel column chromatography of the acetone extracts (C I, C II and C III), were studied by positive ion fast atom bombardment-mass spectrometry (FAB-MS) and hydrogen-nuclear magnetic resonance (H-NMR). Results indicated the presence of (1) a sterol in the yellow colored C I fraction; (2) a mixture of pheophorbides a and b in the major green fraction, C II; and (3) a modified pheophorbide a in the smaller green fraction, C III. Aqueous extracts of the C endosymbiont did not show evidence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Symbiosis; Cyanobacteria; Chloroplast; Photosynthetic pigments; Phylogeny.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000200001

Visualization of the ribosomal DNA (45S rDNA) of Indica rice with FISH on some phases of cell cycle and extended DNA fibers
Provedor de dados: Biocell
Autores: Yun Li,Zong; Li Fu,Mei; Fang Hu,Fang; Feng Huang,Shu; Chun Song,Yun.
The ribosomal DNA (45S rDNA) behaviors during the cell cycle were analyzed on interphase nuclei, prophases, metaphases, pachytene chromosomes and extended DNA fibers in rice (Oryza,sativa ssp.indica cv.Guangluai No.4) by using high-resolution fluorescent in situ hybridization (FISH). The results show that 45S rDNA is located at the ends of short arms of chromosomes 9 and 10. But the signals are much more intense on chromosome 9 than on chromosome 10 in metaphase. Pachytene chromosome has rDNA signal arrays on chromosome 9. Different phases are described and discussed. These results indicate that the activity of rDNA at individual loci may also vary through the cell cycle in rice. On extended DNA fibers, 45S rDNA signals appear as strings of numerous red...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell cycle; Fluorescent in situ hybridization (FISH); Ribosomal DNA (45S rDNA); Pachytene chromosomes; Indica rice.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100004

Reduction of b-Glucuronidase and nitroreductase activity by yoghurt in a murine colon cancer model
Provedor de dados: Biocell
Autores: de Moreno de LeBlanc,A.; Perdigón,G.
Yoghurt feeding inhibits induced colon cancer in mice. Several studies showed the immunomodulatory effect of yoghurt which can explain this inhibition. It is possible that yoghurt bacteria can also affect gut flora enzymes related to colon carcinogenesis as reported for other probiotics in different animal tumours. The aim of this work was to evaluate the role of yoghurt starter bacteria and their cell-free fermentation products on the reduction of procarcinogen enzyme activities (beta-glucuronidase and nitroreductase). Mice injected with 1,2-dimethylhydrazine (DMH) and fed with yoghurt were used for this study. Mice given milk or yoghurt supernatant (cell free extract) were used to evaluate if the yoghurt antitumour effect is due to the starter bacteria...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colon cancer; Yoghurt; Starter bacteria; Beta-glucuronidase; Nitroreductase.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000100003

A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation
Provedor de dados: Biocell
Autores: Capani,Francisco; Saraceno,Ezequiel; Boti,Valeria Romina; Aon-Bertolino,Laura; Fernández,Juan Carlos; Gato,Fernando; Krause,Maria Sol; Giraldez,Lisandro; Ellisman,Mark H.; Coirini,Héctor.
Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correlative fluorescence light microscopy and transmission electron microscopy studies of F-actin distribution are facilitated with this method for morphological and physiological studies. Because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of F-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. The combination of selective staining and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: F-actin; Phalloidin-eosin phootoxidation; 3-D reconstructions; Electron tomography.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000100001

Leaf blade anatomy and ultrastructure of six Simira species (Rubiaceae) from the Atlantic Rain Forest, Brazil
Provedor de dados: Biocell
Autores: Moraes,Tarsila Maria da Silva; Barros,Claudia Franca; Neto,Sebastião José da Silva; Gomes,Valdirene Moreira; Da Cunha,Maura.
Simira is a predominantly woody Neotropical genus comprising 41 taxa, 16 of which occur in Brazil and eight of them in the southeastern region of Brazil. Leaf blades of Simira eliezeriana Peixoto, S. glaziovii (K. Schum.) Steyerm., S. grazielae Peixoto, S. pikia (K. Schum.) Steyerm., S. rubra (Mart.) Steyerm., S. sampaioana (Standl.) Steyerm.were collected in the southeastern region of Brazil and fixed according to usual methods for light and electron microscopy. The leaf blades show typical characteristics of the Rubiaceae family as dorsiventral mesophyll and paracytic stomata. The presence of two bundle sheaths that extend to the upper epidermal layer, prismatic crystal and crystal-sand, alkaloids in the mesophyll and the organization micromorphological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leaf anatomy; Structure; Morph-anatomy.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000300004

Construction of engineered murine Embryonic stem cells with conditional knockout of FGFR2 depending on Cre-loxP
Provedor de dados: Biocell
Autores: Wang,Jianmin; Song,Ruihua; Chen,Lei; Yin,Liangjun; Chen,Bo; Sun,Jing; Gou,Yuanbing; Zhao,Ling; Chen,Lin.
Objective: To investigate the functions of Fibroblast Growth Factor Receptor-2 (FGFR2) at different stages of cell differentiation. The engineered murine embryonic stem (ES) cells with conditional knockout of FGFR2 were developed depending on Cre-loxP. Methods: Cre-loxP system was used in a conditional targeting vector. The competent AM-1 bacteria, which expressed Cre-recombinase, was used to confirm the Cre-mediated deletion of the floxed exons 7 and 8 of FGFR2. The targeting vector was electroporated into the ES cells, and the transfected ES cells were screened with G418 and Ganciclovir. Finally, the ES clones with correct targeting events were identified by Southern Blot and PCR. Results: The targeting vector with conditional knockout of murine FGFR2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibroblast growth factor receptor-2 (FGFR2); Conditional knockout; Homologous recombination; Cre; ES cells.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200002

Effect of morphological heterogeneity of somatic embryos of Melia azedarach on conversion into plants
Provedor de dados: Biocell
Autores: Vila,Silvia; Gonzalez,Ana; Rey,Hebe; Mroginski,Luis.
Embryogenic cultures were initiated from immature Melia azedarach (Meliaceae) zigotic embryos. Explants were induced on Murashige and Skoog (1962) medium with 4.54 &amp;#956;M thidiazuron or 0.45 &amp;#956;M dichlorophenoxyacetic acid. After 6 weeks of culture on induction medium, somatic embryos were categorized in four morphological classes based on the presence of single or fused embryos and if they remained united or not to the original explant; that were evaluated histologically. The somatic embryos of every category were transferred, in groups or individually, on a 1/4 MS medium. Bipolar embryos, the more typically normal ones, had well defined shoot and root apical meristems and produced single plants; subcultured individually their conversion was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Somatic embryo morphology; Conversion; Histology; Scanning electron microscopy.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000100002

Immature oocyte quality and maturational competence of porcine cumulus-oocyte complexes subpopulations
Provedor de dados: Biocell
Autores: Alvarez,Gabriel Martin; Dalvit,Gabriel Carlos; Achi,María Verónica; Miguez,Marcelo Sergio; Cetica,Pablo Daniel.
Porcine immature oocyte quality (i.e., that of live oocytes at the germinal vesicle stage) was evaluated according to features of the surrounding cumulus, aiming to establish maturational competence of different subpopulations of such cumulus-oocyte complexes. Six subpopulations were identified: A1 (with a dense cumulus), A2 (with a translucent cumulus), B1 (with the corona radiata), B2 (partly naked oocytes), C (naked oocytes), D (with a dark cumulus). The percent incidence of live oocyte in these subpopulations changed significantly as related to cumulus features, however the occurrence of oocytes in the germinal vesicle stage was lower in class D only. Similar metaphase II rates achieved in A1, A2, B1 and B2 classes after in vitro maturation suggest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro maturation; Nuclear maturation; Cytoplasmic maturation; Pig.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000300005

Post-treatment with plant extracts used in Brazilian folk medicine caused a partial reversal of the antiproliferative effect of glyphosate in the Allium cepa test
Provedor de dados: Biocell
Autores: Dal-Souto Frescura,Viviane; Wouters Kuhn,Andrielle; Laughinghouse Iv,Haywood Dail; Terezinha Paranhos,Juçara; Bosio Tedesco,Solange.
Species of the genus Psychotria are used for multiple purposes in Brazilian folk medicine, either as water infusions, baths or poultices. This study was aimed to evaluate the genotoxic and antiproliferative effects of infusions of Psychotria brachypoda and P. birotula on the Allium cepa test. Exposure to distilled water was used as a negative control, while exposure to glyphosate was used as a positive control. The interaction of extracts (as a post-treatment) with the effects of glyphosate was also studied. Results showed that glyphosate and the extracts of both P. brachypoda and P. birotula reduced the mitotic index as compared with the negative control (distilled water). Surprisingly, however, both extracts from P. brachypoda and P. birotula caused a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants; Psychotria brachypoda; Psychotria birotula; Cell proliferation; Apoptosis.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000200002

Conchological variation in Pomacea canaliculata and other South American Ampullariidae (Caenogastropoda, Architaenioglossa)
Provedor de dados: Biocell
Autores: Estebenet,Alejandra L.; Martín,Pablo R.; Silvana,Burela.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shell; Operculum; Periostracum; Banding pattern; Apple snail.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200010

Karyotype description of Pomacea patula catemacensis (Caenogastropoda, Ampullariidae), with an assessment of the taxonomic status of Pomacea patula
Provedor de dados: Biocell
Autores: Diupotex-Chong,María Esther; Cazzaniga,Néstor J.; Hernández-Santoyo,Alejandra; Betancourt-Rule,José Miguel.
Mitotic chromosomes of the freshwater snail Pomacea patula catemacensis (Baker 1922) were analyzed on gill tissue of specimens from the type locality (Lake Catemaco, Mexico). The diploid number of chromosomes is 2n = 26, including nine metacentric and four submetacentric pairs; therefore, the fundamental number is FN = 52. No sex chromosomes could be identified. The same chromosome number and morphology were already reported for P. flagellata, i.e., the other species of the genus living in Mexico. The basic haploid number for family Ampullariidae was reported to be n = 14 in the literature; so, its reduction to n = 13 is probably an apomorphy of the Mexican Pomacea snails. Lanistes bolteni, from Egypt, also shows n = 13, but its karyotype is much more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apple snails; Chromosomes; Taxonomy; Phylogeny; Mexico.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000300004

The ovary of Lagostomus maximus (Mammalia, Rodentia): an analysis by confocal microscopy
Provedor de dados: Biocell
Autores: Espinosa,María B.; Fraunhoffer,Nicolás A.; Leopardo,Noelia P.; Vitullo,Alfredo D.; Willis,Miguel A.
Lagostomus maximus is a notable mammalian model for reproductive studies. Females have an extremely high ovulation rate, which is due to down-regulation of the follicular apoptosis pathway, which ensures a large pool of developing follicles. This large pool is supported by the convoluted anatomy of the mature ovary, whose germinal tissue is found in irregularly curved ridges throughout the cortex. Medullary tissue is restricted to a minimum. Lyso Tracker Red reconstruction under confocal laser scanning microscopy was used to recognize and measure all follicular stages from primordial to antral. Unlike most mammals in which early primordial follicles are just found in fetal life, the adult ovary shows regions packed with early primordial follicles. Follicle...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rodents; Germ cells; Three-dimensional analysis.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000200001

Extracellular matrix of ostrich articular cartilage
Provedor de dados: Biocell
Autores: Tomiosso,Tatiana Carla; Gomes,Laurecir; de Campos Vidal,Benedicto; Pimentel,Edson Rosa.
The composition and organization of the extracellular matrix of ostrich articular cartilage was investigated, using samples from the proximal and distal surfaces of the tarsometatarsus. For morphological analysis, sections were stained with toluidine blue and analyzed by polarized light microscopy. For biochemical analysis, extracellular matrix components were extracted with 4 M guanidinium chloride, fractionated on DEAE-Sephacel and analyzed by SDS-PAGE. Glycosaminoglycans were analyzed by electrophoresis in agarose gels. Structural analysis showed that the fibrils were arranged in different directions, especially on the distal surface. The protein and glycosaminoglycan contents of this region were higher than in the other regions. SDS-PAGE showed the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Articular cartilage; Collagen; Decorin; Ostrich; Proteoglycan.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000100007

Tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development
Provedor de dados: Biocell
Autores: Calabrese,Graciela Cristina; Wainstok,Rosa.
Endothelial cells, at the cell-cell borders, express PECAM-1, and have been implicated in vascular functions. The monoclonal antibody MEC 13.3 recognizes PECAM-1 molecule from mouse vessels and allows to analyze the ontogeny of mouse endothelium. At the present, little is known about the molecular basis of differentiation pathways of endothelial cells, that enables its morphological heterogeneity. The purpose of this study was to analyze the pattern of PECAM-1 expression, employing monoclonal antibody MEC 13.3, in cellular suspensions obtained from different mouse organs at pre and postnatal stages. Fluorescence activated cell sorter analysis showed a different profile of the glycoprotein expression in a cell population with size and granularity selected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endothelial cells; PECAM-1; Vasculogenesis; Mouse organs.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000300001

Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I
Provedor de dados: Biocell
Autores: Varecha,Miroslav; Páclová,Daniela; Procházková,Jirina; Matula,Pavel; Cmarko,Dusan; Kozubek,Michal.
Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Superoxide production could be used as a valuable measure of complex I function, because the majority of superoxide is produced there. Therefore, we employed superoxide-specific mitochondrial fluorescence dye for detection of superoxide production. We studied an impact of AIF knockdown on function of mitochondrial complex I by analyzing superoxide production in selected cell lines. Our results show that tumoral telomerase-positive (TP)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Superoxide; Fluorescence microscopy; Image analysis; Mitochondria; Telomerase.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300004

Natural infection of Viola cornuta (Violaceae) with Cucumber mosaic virus, subgroup I
Provedor de dados: Biocell
Autores: Arneodo,Joel; de Breuil,Soledad; Lenardon,Sergio; Conci,Luis.
Plants of Viola cornuta displaying typical virus symptoms were observed during spring 2003 in a plant nursery in Córdoba, central Argentina. Electron microscopic examinations of symptomatic leaf samples revealed the presence of isometric virus-like particles about 30 nm in diameter. Subsequent serological analysis allowed the identification of the pathogen as a subgroup I strain of Cucumber mosaic virus (CMV). These results were confirmed by antigen capture - reverse transcription - polymerase chain reaction with specific CMV primers, and digestion with a restriction enzyme. This is the first report of CMV infecting V. cornuta in Argentina.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Viola cornuta; Cucumber mosaic virus; Electron microscopy; Serology; AC-RT-PCR.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200008

Regulation of mouse embryo development by autocrine throphic factors
Provedor de dados: Biocell
Autores: Teruel,M.T.; Catalano,R.C.; Cabodevila,J.A.; Callejas,S.S.
Embryo development depends on maternal and embryonic factors. When occurs in vitro, embryos secrete factors that stimulate their development. The purpose of this study was to investigate the possible effects of embryos at morula stage on mouse embryo development in vitro. To obtain conditioned media (CM), morulas were cultured in groups of 5 (CM5) or 10 (CM10) in microdrops of Ham-F10 culture medium during 24 h and later they were removed. Subsequently, 365 morulas were cultured in CM5 and CM10 or in Ham-F10 media (as control group). No differences in blastocyst formation could be found between embryos cultured for 24h in Ham-F10, CM5 or CM10 (49.66, 53.04, 60.00% respectively). However, CM5 significantly increased differentiation in embryos cultured for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo development; Mouse; Autoregulation; Trophic factors.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200005

Angiotensin II type 1 receptor A1166C GENE polymorphism and essential hypertension in San Luis
Provedor de dados: Biocell
Autores: Lapierre,Alicia Viviana; Arce,María Elena; Lopez,José Raúl; Ciuffo,Gladys María.
Essential hypertension is considered a multifactorial trait resulting from a combination of environmental and genetic factors. The angiotensin II type 1 receptor mediates the vasoconstrictor and growthpromoting effects of Ang II. The A1166C polymorphism of the AT1 receptor gene may be associated with cardiovascular phenotypes, such as high arterial blood pressure, aortic stiffness, and increased cardiovascular risk. We investigated the association between this A1166C polymorphism and hypertension in hypertense and normotense subjects from San Luis (Argentina) by mismatch PCR-RFLP analysis. Hypertense patients exhibited significant increases in lipid related values and body mass index. The frequency of occurrence of the C1166 allele was higher among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin II; A1166C polymorphism; Risk factors; RAS system; Pharmacogenetics.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000300005

Water relations and leaf growth rate of three agropyron genotypes under water stress
Provedor de dados: Biocell
Autores: García,María G.; Busso,Carlos A.; Polci,Pablo; García Girou,Norberto L.; Echenique,Vivivan.
The effects of water stress on leaf water relations and growth are reported for three perennial tussock grass genotypes under glasshouse conditions. Studies were performed in genotypes El Palmar INTA and Selección Anguil of Agropyron scabrifolium (Döell) Parodi, and El Vizcachero of A. elongatum (Host) Beauv. Agropyron scabrifolium El Palmar INTA is native to a region with warm-temperate and humid climate without a dry season, and an average annual precipitation of 900 mm. Agropyron scabrifolium Selección Anguil comes from a region with a sub-humid, dry to semiarid climate and a mean annual precipitation of 600 mm. Agropyron elongatum is a widespread forage in semiarid Argentina with well-known water stress resistance. A mild water stress treatment was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agropyron scabrifolium (Döell) Parodi; Agropyron elongatum (Host) Beauv; Water stress; Water relations; Leaf growth rate.
Ano: 2002
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452002000300001

Pollen viability of Polygala paniculata L. (Polygalaceae) using different staining methods
Provedor de dados: Biocell
Autores: Frescura,Viviane Dal-Souto; Laughinghouse IV,Haywood Dail; Dorow,Thais Scotti do Canto-; Tedesco,Solange Bosio.
Polygala paniculata L. is a medicinal plant that grows in the Brazilian Atlantic coast, known as 'barba-de-São-João', 'barba-de-bode', 'vassourinha branca', and 'mimosa'. In this study, pollen viability was estimated by three different staining methods: 2% acetic orcein, 2% acetic carmine, and Alexander's stain. The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol: acetic acid (3:1) for 24 hours, then stored in ethanol 70% under refrigeration. Six slides per plant, two for each stain, were prepared by squashing, and 300 pollen grains per slide were analyzed. Pollen viability was high (&gt;70%) for most accessions of P. paniculata using the Alexander's stain, which proved the most adequate method to estimate pollen...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Pollen grains; Barba-de-bode; Acetic orcein; Acetic carmine; Alexander's stain.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300007

Spherites in the midgut epithelial cells of the sugarcane borer parasitized by Cotesia flavipes
Provedor de dados: Biocell
Autores: de Oliveira Pinheiro,Daniela; Conte,Hélio; Aparecida Gregório,Elisa.
Diatraea saccharalis, the main pest of sugarcane, has been controlled by Cotesia flavipes. Very little is known about the effect of parasitism on the host organs, including the midgut. The Lepidoptera midgut epithelium is composed of columnar, goblet, regenerative, and endocrine cells. Spherites have been described in columnar and regenerative cells of several Lepidoptera species, and presented a lot of functional meaning. We identified spherites in the midgut epithelial cells of non-parasitized D. saccharalis larvae analyzed the effect of parasitism on spherite morphology and distribution along the length of the midgut. Midgut fragments of both non-parasitized and parasitized larvae were processed for transmission electron microscopy. All the midgut...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spherite; Epithelial cell; Ultrastructure; Lepidoptera; Parasitism.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000100007

Production of "pejerrey" Odontesthes argentinensis fingerlings: A review of current techniques
Provedor de dados: Biocell
Autores: Sampaio,Luís André.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquaculture; Reproduction; Larviculture; Atherinopsidae.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100015

CD44 is involved in CXCL-12 induced acute myeloid leukemia HL-60 cell polarity
Provedor de dados: Biocell
Autores: Zhou,Liping; Guo,Xiaolin; Ba,Jing; Zhao,Lianshuang.
CXCL-12 and its receptor CXCR4 participate in breast cancer and melanoma cell metastasis to bone and lymphoid nodes. CD44, as a receptor for hyaluronic acid, is involved in lymphocyte recirculation, homing, adhesion and migration. But the role of CD44 in CXCL-12 induced leukemia cell migration still remains unclear. The present study showed that CXCL-12 stimulation induced the rapid internalization of CXCR4 and facilitated the formation of lamellipodia and uropod in acute leukemia cell line HL-60. CXCL-12 also induced CD44 translocation into the uropod, while CD44 remained evenly distributed on the untreated cell membranes. Results suggest that CD44 participates in CXCL-12 induced cell polarization and subsequent cell migration.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Cell migration; Lamellipodia formation; Uropod formation; Hyaluronic acid.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000200005

Dr. Francisco Bertini (1932-2006)
Provedor de dados: Biocell
Tipo: Info:eu-repo/semantics/other
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100009

Sperm ultrastructure of Mytella (Bivalvia) populations from distinct habitats along the northern coast of São Paulo State, Brazil
Provedor de dados: Biocell
Autores: Orlandi Introíni,Gisele; Martins Maester,Fernanda; Pedini Pereira Leit,Fosca; Recco-Pimentel,Shirlei Maria.
Ultrastructural analyses of bivalve spermatozoa are relevant in studies that aim to identify taxonomic traits for the purposes of discriminating species and conducting phylogenetic studies. In the present work, spermatozoa of mussel specimens of the genus Mytella, collected from two populations living in distinct habitats, were examined by electron microscopy. The objective was to identify sperm ultrastructural taxonomic traits that could be used to differentiate Mytella species. The specimens were from populations that live in intertidal zones on the southeast coast of Brazil, either buried in muddy-sand sediment or anchored to rocky substrates. The acrosomal vesicle was conical and long, the axial rod extended from the nucleus to the acrosome, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mytilidae; Spermatozoa; Electron microscopy; Mussel.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000300002

Isolation, culture, characterization and optimization of human corneal stem cells
Provedor de dados: Biocell
Autores: Sharifi,Ali M.; Darabi,Radbod; Jadidi,Khosrow.
The effects of human versus mouse EGF on cell growth and culture duration were studied to optimize a human limbal stem cells culture method for therapeutical autologous transplantation. Limbal cells were obtained by trypsin digestion and transferred to a culture medium. The time needed to reach full confluence in culture was determined. Specific antibodies to corneal stem cell marker (P63) versus corneal epithelial differentiation marker (K3) were used for histochemical determinations. A high proportion of P63 positive cells (85± 4.6%), and a correspondingly low proportion K3 positive cells (15 ± 3.8%) indicated that most cultured cells remained undifferentiated and were considered as stem cells (mean ± SE, n=10). Cultures reached full confluency after...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Limbal stem cells; Limbal stem cells deffiency (LSCD); Human EGF; Mouse EGF.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000100007

Antioxidant capacity of vitamin C in mouse liver and kidney tissues
Provedor de dados: Biocell
Autores: Weyers,Alicia; Ugnia,Laura I.; García Ovando,Hugo; Gorla,Nora B.
In the present study, the antioxidant capacity of vitamin C was examined in the liver and the kidney tissues of mice with or without ciprofloxacin (CFX) treatment. The antioxidant capacity of the vitamin was evaluated in terms of lipid hydroperoxides (LOOH) and thiobarbituric acid reactive substances (TBARs). The experimental design was 15 days of water (control and CFX groups) or vitamin C (vitamin C and vitamin C plus CFX groups) in drinking water. One dose of CFX was injected, 15 minutes before sacrifice, in the corresponding mice. The initial nmol of lipid hydroperoxides/g of tissue were 137 ± 11 in the kidney and 145 ± 15 in the liver, and the nmol of TBARs were 13 ± 0.7 and 12 ± 0.6, respectively. Pre-treatment with vitamin C reduced the levels of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipid peroxidation; Vitamin C; Ciprofloxacin; Mice.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000100003

Rosette formation by macrophages with adhered T lymphocytes is precluded by inhibitors of antigen processing and presentation
Provedor de dados: Biocell
Autores: Novak,Ivón Teresa; Cabral,Humberto Ramón.
We had previously found in autologous human leukocyte cultures, in which dead neutrophils phagocytosis by macrophages occur, macrophages and T CD4 lymphocytes perform a selective cell-cell interaction showing many figures of either one, two or several T- lymphocytes adhering to a central macrophage were seen. Considering that antigen presentation would be necessary for the formation of these immune synapses, we attempted to block rosette formation (i.e., the formation of macrophage associations with at least three lymphocytes) by interfering with both antigen processing and presentation. Culture samples of autologous leukocytes from 7 healthy donors were subjected to either brefeldin A, chloroquine or to an anti- HLA DR antibody. Rosette formation was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autologous rosetting; Cultured human macrophages and T- lymphocytes; Brefeldin A; Chloroquine; Immune synapses.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200002

Assessment of gonadal follicle size in the invading bivalve Limnoperna fortunei (Mytilidae)
Provedor de dados: Biocell
Autores: Guimarey,Pilar; Darrigran,Gustavo; Damborenea,Cristina; Penchaszadeh,Pablo E.
Limnoperna fortunei is an invasive gonochoristic and byssate freshwater bivalve originary from Southeast Asia. It shows great adaptive-reproductive ability, so knowledge of the gonadal cycle is an important factor for the prevention and control of this bioinvasion. This species is highly damaging to natural and human environments. We analyzed the distribution and maturity state of reproductive follicles in the mantle of both male and females. Male results are not shown but, in general, they followed the same pattern as that of females. Routine histological techniques included serial longitudinal sections and transversal sections in three body regions (anterior, middle and psoterior). Oocytes with conspicuous nucleoli were measured on both types of sections...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Golden mussel; Gonadal cycle; Bioinvasion; Control.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000200004

Synaptonemal complexes and XY behavior in two species of argentinian armadillos: Chaetophractus villosus and Dasypus hybridus (Xenarthra, Dasypodidae)
Provedor de dados: Biocell
Autores: Sciurano,R. B.; Merani,M.S.; Bustos,Jimena; Solari,A.J.
Spermatocytes from the two armadillo species, C. villosus and D. hybridus were studied in microspreads for synaptonemal complexes (SCs) and in thin sections for electron microscopy (EM). The complete SC karyotype generally agrees with previous reports on mitotic chromosomes, except for the sex chromosomes. The X chromosome is submetacentric in both species and the Y is the shortest one in C. villosus and the second shortest in D. hybridus, and an extremely acrocentric one. A SC is formed along the total length of the Y chromosome, and this SC persists along all the pachytene substages. A single ecombination nodule (RN) is located in the region of the SC nearest to the attachment to the nuclear envelope. The lateral element (LE) of the X axis in the SC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xenarthra; XY chromosomes; Meiosis; Synaptonemal complexes.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100009

Structure and secretory activity of cultured chondrocytes from patients with osteoarthritis
Provedor de dados: Biocell
Autores: Montrull,Hilda L.; Brizuela,Nilda Y.; Demurtas,Silvia L.; Spitale,Luis; Meirovich,Carlos I.
Cartilage samples were taken from OA patients in order to describe and quantify pro-inflammatory mediators. Samples were cultured under aseptic conditions in Dulbecco's modified Eagle medium at 37°C for 10 days. Control samples, taken from non-inflammatory cartilage, were cultured under the same conditions. The levels of NO-2 and NO-3 were measured in the supernatant using a spectrophotometric assay. The activity of MMP-1 was quantified by ELISA. The concentration of NO-x was 47.3 ± 4.1 µM in the OA cartilague and 10.7 ± 1.8 µM in the controls. The average MMP-1 activity was 3,650 ± 387 ng/ml in the OA cartilage and 2,150 ± 190 ng/ml in the control samples. These increased values of MMP-1 and NO-x observed in the OA cartilage suggest a higher catabolic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoarthritis; Chondrocytes; Collagenase.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200002

Molecular cloning and characterization of a novel mannosebinding lectin cDNA from Zantedeschia aethiopica
Provedor de dados: Biocell
Autores: Zhonghai,Chen; Yongzhen,Pang; Liu,Xiaojun; Wang,Xinglong; Deng,Zhongxiang; Sun,Xiaofen; Tang,Kexuan.
Using RNA extracted from Zantedeschia aethiopica young leaves and primers designed according to the conservative regions of Araceae lectins, the full-length cDNA of Z. aethiopica agglutinin (zaa) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of zaa was 871 bp and contained a 417 bp open reading frame (ORF) encoding a lectin precursor of 138 amino acids. Through comparative analysis of zaa gene and its deduced amino acid sequence with those of other Araceae species, it was found that zaa encoded a precursor lectin with signal peptide. Secondary and three-dimensional structure analyses showed that ZAA had many common characters of mannose-binding lectin superfamily and ZAAwas a mannose-binding lectin with three mannose-binding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mannose-binding lectin; RACE; Zantedeschia aethiopica; ZAA.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200006

The effect of transportation stress on tissue ascorbic acid levels of Mexican silverside (Chirostoma estor estor Jordan, 1979)
Provedor de dados: Biocell
Autores: Ríos-Durán,M. G.; Hernández-Téllez,A. R.; Martínez-Palacios,C. A.; Ross,L. G.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascorbic acid tissue levels; Mexican silverside; Transport stress.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100019

Advances in applied research for the culture of Mexican silversides (Chirostoma, Atherinopsidae)
Provedor de dados: Biocell
Autores: Martínez Palacios,C.A.; Racotta,I.S.; Ríos-Durán,M.G.; Palacios,E.; Toledo-Cuevas,M.; Ross,L.G.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chirostoma estor estor; Larviculture; Buccal anatomical structures; Optimal culture conditions.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100018

Morphology and histology of P. argentinus (Crustacea, Decapoda, Caridea) digestive tract
Provedor de dados: Biocell
Autores: Sousa,Liliana; Petriella,Ana María.
This work describes the morphology and histology of the P. argentinus digestive tract. The foregut comprises the mouth, oesophagus, and stomach and is lined by a simple cylindrical epithelium overlain by cuticle. There are tegumental glands in the oral region and in the first portion of the oesophagus and of the hindgut. The cardiac stomach is an oval dorsal sac in the cephalothorax and has no calcified structures. The pyloric stomach comprises an upper chamber and a lower gland filter. The filter consists of an outer row of elongated setae and an inner row of dorsally curved setae forming longitudinal channels 16-18µm wide. The midgut runs from the dorsal chamber of the pyloric stomach to the sixth abdominal somite without caeca. The hindgut runs from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crustacea; Caridea; Morphology; Histology; Digestive tract.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200004

Immunophenotypic evaluation, and physiological and laboratory correlations of hematopoietic stem cells from umbilical cord blood
Provedor de dados: Biocell
Autores: Canabarro,Raquel; Sporleder,Heloísa; Gomes,Thaís; Zanatta,Geancarlo; Scribel,Letícia; Freitas,Fernando; Neumann,Jorge; Pranke,Patricia.
The use of umbilical cord blood stem cells is an efficient alternative for the transplantation of hematopoietic progenitor cells. A number of factors can influence the volume and amount of CD34+ cells, which are considered as immature and capable of proliferation. Quantification of CD34+ cells, evaluation of CD38 and c-kit molecules on these cells, as well as correlations of such factors as maternal age, gestational age, newborn sex and weight, umbilical cord length, placental weight with increased volume and concentration of immature cells, among others, were performed in 70 blood samples from term newborns. The mean volume of umbilical cord blood collected was 53.8±33.6 mL, where 30.96±18.9 CD34+/mL UCB cells were found, of which 16.66±8.32% were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematopoietic stem cells; Immunophenotyping; Placental/umbilical cord blood.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300006

Human umbilical artery smooth muscle exhibits a 2-APB-sensitive capacitative contractile response evoked by vasoactive substances and expresses mRNAs for STIM, Orai and TRPC channels
Provedor de dados: Biocell
Autores: Roldán Palomo,Ana Rocío; Martín,Pedro; Rebolledo,Alejandro; Enrique,Nicolás; Flores,Luis E.; Milesi,Verónica.
After depletion of intracellular Ca2+ stores the capacitative response triggers an extracellular Ca2+ influx through store-operated channels (SOCs) which refills these stores. Our objective was to explore if human umbilical artery smooth muscle presented this response and if it was involved in the mechanism of serotonin- and histamine-induced contractions. Intracellular Ca2+ depletion by a Ca2+-free extracellular solution followed by Ca2+ readdition produced a contraction in artery rings which was inhibited by the blocker of Orai and TRPC channels 2-aminoethoxydiphenyl borate (2-APB), suggesting a capacitative response. In presence of 2-APB the magnitude of a second paired contraction by serotonin or histamine was significantly less than a first one,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human umbilical artery; Capacitative response; TRPC channels; Serotonin; Histamine.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000200004

Seasonal testicular changes in Dendropsophus minutus Peters, 1872 (Anura, Hylidae)
Provedor de dados: Biocell
Autores: Ferreira,Adelina; Mehanna,Mahmoud.
The reproductive cycle in anurans may be either continuous or discontinuous. These differences may be connected to seasonal climate changes and/or to anthropic activity. Forty adult male individuals of the Dendropsophus minutus species were collected during one year, in the municipality of Chapada dos Guimarães (Mato Grosso, Brazil). The testicles were studied under light and transmission electron microscopy. No variations were observed when the diameter of the seminiferous tubules and the thickness of the interstitial tissue were studied. However, changes in spermatogenesis were conspicuous and indicated that the reproductive cycle of D. minutus in Chapada dos Guimarães is discontinuous and seems related to variations in air temperature and rainfall.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spermatogenesis; Reproduction; Anura; Ultrastructure; Chapada dos Guimarães.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000200002

Prolonged exposure of human embryonic stem cells to heat shock induces necrotic cell death
Provedor de dados: Biocell
Autores: Heng,Boon Chin; Vinoth,Kumar Jayaseelan; Lu,Kai; Deng,Xuliang; Ge,Zigang; Bay,Boon Huat; Cao,Tong.
We investigated the effects of prolonged heat shock treatment on human embryonic stem cell (hESC) viability. The hESC viability steadily declined with longer exposure to heat shock treatment (43ºC). After 4 h of exposure to heat shock at 43ºC, only 56.2 ± 1.5% of cells were viable. Viability subsequently declined to 37.0 ± 3.3% and 3.5 ± 0.7% after 8 h and 16 h, respectively of heat shock treatment at 43ºC. Transmission electron micrographs showed that the morphology of the dead/dying cells after heat shock treatment was characteristic of cellular necrosis with an uncondensed chromatin and a non-intact plasma membrane. This was further confirmed by flow cytometry analysis which showed that the DNA of the dead/ dying cells was still mostly intact, unlike...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryonic; Heat shock; Human; Necrosis; Stem cells.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300007

Reactive oxygen species in bovine embryo in vitro production
Provedor de dados: Biocell
Autores: Dalvit,G.C.; Cetica,P.D.; Pintos,L.N.; Beconi,M.T.
Oxidative modifications of cell components due to the action of reactive oxygen species (ROS) is one of the most potentially damaging processes for proper cell function. However, in the last few years it has been observed that ROS participate in physiological processes. The aim of this work was to determine ROS generation during in vitro production of bovine embryos. Cumulus-oocyte complexes were recovered by aspiration of antral follicles from ovaries obtained from slaughtered cows and cultured in medium 199 for 22 h at 39ºC in 5% CO2: 95% humidified air. In vitro fertilization was carried out in IVF-mSOF with frozenthawed semen in the same culture conditions and embryo in vitro culture in IVC-mSOF at 90% N2: 5% CO2: 5% O2. ROS was determined in denuded...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Reactive oxygen species; Oxidative stress; Oocyte; Embryo; Bovine.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200009

Silversides in South Brazil: Morphological and ecological aspects
Provedor de dados: Biocell
Autores: Bemvenuti,M. A.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silversides; Odontesthes; Brazil; Morphology; Multivariate.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100013

Examination of camptothecin and 10-hydroxycamptothecin in Camptotheca acuminata plant and cell culture, and the affected yields under several cell culture treatments
Provedor de dados: Biocell
Autores: Pi,Yan; Jiang,Keji; Hou,Rong; Gong,Yifu; Lin,Juan; Sun,Xiaofen; Tang,Kexuan.
Camptothecin and its derivatives are monoterpenoid indole alkaloids exhibiting significant anti-tumor actions. With the aim of improving the production of these pharmaceuticals, the contents of camptothecin and 10-hydroxycamptothecin in different tissues including roots, stems, leaves, young flower buds, opening flowers, fading flowers and seeds from Camptotheca acuminata, were investigated. The young flower buds had the highest alkaloid concentrations (camptothecin, 2.46 mg/g of dry weight; 10-hydroxycamptothecin, 1.41 mg/g of dry weight). Callus showed lower concentrations but it should also be considered as a potential source of these pharmaceuticals. In the present study, the growth rate of Camptotheca acuminata cells in culture did not correlate with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPLC; Salicylic acid; UV-B.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000300006

Proacrosin-acrosin activity in capacitated and acrosome reacted sperm from cryopreserved bovine semen
Provedor de dados: Biocell
Autores: Rosatti,M. I.; Beconi,M. T.; Córdoba,M.
Acrosin activity is associated with normal fertility in human and bovine spermatozoa. The aim of the study was to determine the variation of the enzyme activity in the proacrosin-acrosin system in capacitated and acrosome reacted cryopreserved bovine sperm. Enzyme activity was assessed spectrophotometrically using N-a-benzoyl-DL-arginine p-nitroanilide (BAPNA) as specific substrate for acrosin at pH 8. Capacitation with heparin and quercitin failed to induce conversion of proacrosin to acrosin. An increase in acrosin activity produced by the presence of progesterone, in a dose-dependent manner, was related with the induction of true acrosome reaction. The total level of acrosin activity registered showed that 96% of acrosin of capacitated sperm samples and...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Bovine sperm; Proacrosin-acrosin; Heparin; Quercitin; Progesterone.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000300007

Apigenin inhibits cell migration through MAPK pathways in human bladder smooth muscle cells
Provedor de dados: Biocell
Autores: Qingxin,Liu; Xianggui,Chen; Guolin,Yang; Xuewen,Min; Maoxian,Deng.
Apigenin, a nonmutagenic flavonoid, has been shown to possess free radical scavenging activities, anticarcinogenic properties, antioxidant and anti-inflammatory effects. Recently, apigenin was reported to cause gastric relaxation in murine. To assess possible effects of apigenin on migration of bladder smooth muscle (SM) cell, we isolated SM cells from peri-cancer tissue of human bladder and established a cell model that was capable to overexpress transiently MEKK1 (MEK kinase 1). Results showed that overexpression of active human MEKK1 by adenoviruses infection induced migration of human bladder smooth muscle (hBSM) cells and phosphorylation of MAPKs, ERK, JNK and p38, which are the downstream molecules of MEKK1. Then, hBSM cell overexpressing MEKK1 were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flavonoid; Cell motility; MAP kinase.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000300002

Histomorphological and quantitative immunohistochemical changes in the rat pancreas during aging
Provedor de dados: Biocell
Autores: Riccillo,F.L.; Bracamonte,M.I.; Cónsole,G.M.; Gómez Dumm,C.L.A.
Although the endocrine pancreas is the purpose of several deep investigations, morphological data referred to the effect of aging on the gland are not homogeneous. The purpose of the current work was to analyze the changes occurring in the pancreas of aged rats, with especial reference to the islet cell populations. Six young (Y), old (O) and senescent (S) male Sprague-Dawley rats were used. The pancreas tails were processed for light microscopy and studied by means of routine stains as well as by immunohistochemical identification of insulin-, glucagon-, somatostatin-, and pancreatic polypeptide- secreting cells (Dako Envision System, DAB as chromogen). A progressive pancreatic histoarchitecture distortion was found among the aged animals. Even when the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Pancreas; Islets; Immunohistochemistry; Morphometry.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000200001

Bovine parthenogenotes produced by inhibition of first or second polar bodies emission
Provedor de dados: Biocell
Autores: Bevacqua,Romina J.; Fernandez-Martin,Rafael; Salamone,Daniel F.
Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. Only one polar body was inhibited in each strategy in order to keep the diploid chromosome set. In experiment 1, the effect of cytochalasin B on in vitro maturation of bovine oocytes was evaluated. Most oocytes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meiotic maturation; Microfilaments; Cytochalasin B; Parthenogenesis.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000100001

Structure of the stigma and style in sunflower (Helianthus annuus L.)
Provedor de dados: Biocell
Autores: Gotelli,M.M.; Galati,B.G.; Medan,D.
This is the first report of the ultrastructure of the stigma and style during and after anthesis in Helianthus annuus L. using light and transmission electron microscopy. The stigma is bifid with unicellular papillae. There is no secretion of lipids, carbohydrates or proteins at anthesis. The style is semisolid in the upper portion, closer to the stigma, and becomes solid below. Ultrastructural changes on cells of the stigma and the style are described. The transmitting tissue of the ovule is first evident 40 minutes after pollination and persists during the first stages of embryogenesis. Only one pollen tube per micropyle was observed growing through this tissue.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ultrastructure; Gynoecium; Ovule; Transmitting tissue.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000300005

Extracellular breakdown of collagen by mice decidual cells. A cytochemical and ultrastructural study
Provedor de dados: Biocell
Autores: Godosevicius Katz,Sima.
The interaction of antimesometrial decidual cells and collagen fibrils was studied by light microscopy and ultrastructural cytochemistry in fed and acutely fasted mice on days 9-11 of pregnancy. Fibrillar elements in the extracellular space consisted of collagen fibrils and filamentous aggregates (disintegrating collagen fibrils). Intracellular vacuoles exhibited typical collagen immersed in electron-translucent material (clear vacuoles) and faint cross-banded collagen immersed in electron-opaque material (dark vacuoles). Fibrillar elements showed extracellular acid phosphatase activity which was stronger in the region of mature decidua than in predecidual cells region in all animals; it was conspicuous in mature decidua of fasted animals. Intracellular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Decidua; Acid phosphatase; Fasting; Electron microscopy; Mice.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300003

A glass bead protocol for recovery of host cell free Ehrlichia canis and quantification by Sybr-green real-time PCR
Provedor de dados: Biocell
Autores: Cardozo,G. P.; Santos,E. V.; Fachin,A. L.; França,S. C.; Marins,M.
E. canis infection of the canine cell line DH82 is a routine in studies with this bacteria. A protocol for isolation of host cell free bacteria was developed based on the use of glass beads. Improvement of infection with E. canis isolated by this method was detected by real-time PCR.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Ehrlichia canis; Glass bead; Real-time PCR.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000100005

Abstracts from XXVIII Annual Scientific Meeting (In memoriam Dr. Juan Carlos Fasciolo): October 06-08, 2010 Ciudad de Mendoza, Argentina
Provedor de dados: Biocell
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000100006

Bacteriostatic action of synthetic polyhydroxylated chalcones against Escherichia coli
Provedor de dados: Biocell
Autores: Alvarez,María De Los Angeles; Zarelli,Valeria E. P.; Pappano,Nora B.; Debattista,Nora B.
In previous work the bacteriostatic action of trihydroxylated chalcones against Staphylococcus aureus ATCC 25 923 was investigated. In this work the action of 2´,4´,2-(OH)3-chalcone, 2´,4´,3-(OH)3- chalcone and 2´,4´,4-(OH)3 -chalcone against Escherichia coli ATCC 25 922 was evaluated. Growth kinetic curves of E.coli were made in nutritive broth added with increasing drug concentrations. The specific growth rates of the microorganisms were calculated by a kinetic turbidimetric method, which was previously probed and the minimal inhibitory concentrations (MIC´s) were evaluated by a mechanism of action proposed. The MICs of 2´,4´,3-(OH)3-chalcone and 2´,4´,2-(OH)3-chalcone were 46 mg/ml and 122 mg/ml, respectively. The 2´,4´,4-(OH)3-chalcone was inactive....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chalcones; Bacteriostatic Activity; Hydroxylation.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000100004

Evaluation of potential embryo toxicity of albendazole sulphoxide in CF1 mice
Provedor de dados: Biocell
Autores: Teruel,Miriam; D'Ercole,Jaqueline; Catalano,Rodolfo.
Benzimidazole compounds are used in both humans and animals for controlling helminth parasites. Albendazole has teratogenic effects attributed to its active metabolite albendazole sulphoxide. The aim of this work was to evaluate the effect of the latter compound when administered to pregnant CF1 mice during the preimplantation period. Females were superovulated by intraperitoneal injection of 10 IU of eCG and 10 IU of hCG (48h later) and were paired with males of proven fertility. Albendazole sulphoxide (200 mg/kg) was orally administered by gavages at day 1, 2 or 3 of pregnancy; the control group received only the vehicle (carboxymethylcellulose). Females were killed by cervical dislocation at day 4 of pregnancy and embryos were flushed from uteri with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Benzimidazole; Early pregnancy; Developmental exposure.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000100004

Salinity induced anatomical and morphological changes in Chloris gayana Kunth roots
Provedor de dados: Biocell
Autores: Céccoli,Gabriel; Ramos,Julio C.; Ortega,Leandro I.; Acosta,Juan M.; Perreta,Mariel G.
Chloris gayana Kunth is a grass species valuable as forage which was introduced into Argentina to be used as pasture in saline soils of subtropical and warm-temperate zones, given its good adaptability to drought, salinity and mild freezing. However, its tolerance varies according to the cultivar. In tetraploid cultivars, important reductions in yield have been observed. Here, a study of the variations produced on the root and stem system by salinity at different NaCl concentrations (0, 150 y 250 mM) was performed in the Boma cultivar, with the aim of determining the anatomical and morphological alterations produced by the salt excess. Plants cultivated with the highest level of salinity showed, in the whole, significant differences in the measured...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abiotic stress; Tolerance; Vascular tissue.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000100002

Interleukin-1&amp;#946; regulates metalloproteinase activity and leptin secretion in a cytotrophoblast model
Provedor de dados: Biocell
Autores: Fontana,Vanina Andrea; Sanchez,Melisa; Cebral,Elisa; Calvo,Juan Carlos.
Implantation is one of the most regulated processes in human reproduction, by endocrine and immunological systems. Cytokines are involved in embryo-maternal communication and an impaired balance could result in pregnancy loss. Here we investigated the effect of interleukin 1-&amp;#946; on the activity of two important metalloproteinases (MMP-2 and MMP-9) that are involved in extracellular matrix remodeling as well as the secretion of leptin, one of the reproductive hormones actively regulating their activity and secretion. We found that IL-1&amp;#946; activates matrix metalloproteinase activity as well as increases leptin secretion. We propose that this interleukin, through the regulation of leptin, in turn activates matrix metalloproteinases which results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Embryo implantation; Placenta; Extracellular matrix.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000100005

Heme metabolism enzymes are dynamically expressed during Xenopus embryonic development
Provedor de dados: Biocell
Autores: Shi,Jianli; Mei,Wenyan; Yang,Jing.
As the key component of many hemoproteins (heme-containing proteins), heme is involved in a broad range of biological processes. Enzymes required for heme biosynthesis and degradation pathways are evolutionarily conserved. While heme metabolism has been studied extensively, the expression of heme metabolism enzymes during development has not been described. Here, we report that all heme biosynthases and two heme oxygenases, which initiate heme degradation, are dynamically expressed during Xenopus embryonic development. All heme synthases, with the exception of aminolevulinic acid synthase 2, are maternally expressed. At neurula stage, heme synthases are expressed in the developing neural tissue and in migrating neural crest cells. At the swimming tadpole...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heme synthases; Heme oxygenases; Development; Xenopus.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000300007

Construction and application of a yeast expression system for thymosin a1
Provedor de dados: Biocell
Autores: Chen,Feng; Chen,Xiang-Ming; Chen,Zhi; Jiang,Han-Liang; Pan,Xiao-Ping; Hu,Zhong-Rong; Liu,Rong-Hua; Chen,Xiao-Ming.
We want to construct a yeast expression system for thymosin a1 (Ta1) to make the orally administered Ta1 preparation possible. The whole Ta1 DNA fragment was obtained by PCR. After being digested with restriction enzymes, it was cloned into pYES2 vector. Sequencing was performed to identify the recombinant. The sequence of Ta1 in recombinant coincided with the original one reported in Genbank. When pYES2-Ta1 plasmid was transformed into yeast, galactose instead of glucose was used to induce Ta1 expression. Western blot was performed to identify the quality of the expressed Ta1. Dried yeast containing pYEST2-Ta1 was fed to Balb/c mice whose immunities were inhibited by cyclophosphamide in advance. Synthesized Ta1 peptide was used as positive control and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thymosin a1; Yeast expression system; Biological activity; Orally administered.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300002

Chronic stress effects on the apoptotic index of the adrenal cortex of pregnant rats
Provedor de dados: Biocell
Autores: Bozzo,Andrea; Soñez,Carlos Alberto; Mugnaini,María Teresa; Pastorino,Isabel Cecilia; Rolando,Alicia Nélida; Romanini,María Cristina; Gauna,Héctor Fernando.
Chronic stress by immobilization during gestation can alter several mechanisms that maintain homeostasis in adrenal gland. The aim of this work was to quantify the apoptotic index of adrenal cortex during mid-pregnancy and to prove cytological characteristics by electron microscopy. The apoptotic index did not present significant differences between the adrenal cortex areas of control and experimental rats in any of the three ages studied. The day of gestation influenced significantly on the apoptotic index in both groups. This index increased as gestation progressed. It may be concluded that chronic stress by immobilization might induce the increase of apoptotic index in adrenal cortex as gestation progresses which might be related variations of plasmatic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic stress; Apoptosis; Adrenal cortex; Pregnancy.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000300004

Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation
Provedor de dados: Biocell
Autores: Julianelli,Vanina L; Guerra,Liliana N; Calvo,Juan C.
Interaction between parenchyma and stroma is essential for organogenesis, morphogenesis, and differentiation. Mammary gland has being the chosen model for developmental biologist because the most striking changes in morphology and function take place after birth. We have demonstrated a regulation of triglyceride accumulation by protein factors synthesized by normal mouse mammary gland epithelial cells (NMMG), acting on a cell line, 3T3-L1, long used as a model for adipogenesis. In this paper, we demonstrate that this inhibitory effect seems to be shared by other cells of epithelial origin but not by other cell types. We found a regulation of cell proliferation when NMMG cells are cultured in the presence of conditioned media from Swiss 3T3 or 3T3-L1 cells....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triglycerides; Mammary epithelium; Differentiation.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000200006

Variable number tandem repeats in the promoter region of prostacyclin synthase gene in choline deficient rats
Provedor de dados: Biocell
Autores: Denninghoff,Valeria C.; Ossani,Georgina P.; Uceda,Ana M.; Avagnina,Maria A.; Elsner,Boris; Monserrat,Alberto J.
Weanling Sprague-Dawley rats were fed on a choline-deficient diet with hydrogenated vegetable oil and corn oil as lipids develop acute renal failure. Pathogenesis of the latter is controversial and an ischemic mechanism has been proposed. Arachidonic acid derivatives are involved in the regulation of vascular tonus. Vasospasm could be due to an increase in tromboxane A2-mediated vasoconstriction or to a decrease in prostacyclin-induced vasodilatation. Enzymes involved in the synthesis of both compounds are tromboxane A2- and prostacyclin-synthase respectively. The aim of this study was to identify the variable number tandem repeats (VNTR) in the promoter region of prostacyclin synthase gene and verify if there exists a relationship between the occurrence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: VNTR; Prostacyclin synthase gene; Kidney.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000200002

Microsporogenesis in tetraploid accessions of Brachiaria nigropedata (Ficalho &amp;amp; Hiern) Stapf (Gramineae)
Provedor de dados: Biocell
Autores: Sayuri Utsunomiya,Karina; Suely Paglirini,Maria; Borges do Valle,Cacilda.
The genus Brachiaria (Trin.) Griseb. has achieved considerable importance to cattle production systems, as a result of the good production and adaptation of a few cultivars to poor and acid soils of the Brazilian savannas. Many of its species and accessions are polyploid and apomictic, which limits direct hybridization. To assist the breeding program, cytogenetic characterization has been undertaken on the accessions of Brachiaria collection at the Embrapa Beef Cattle Research Center. In this study, chromosome number and meiotic behavior are reported for the Brachiaria nigropedata (Ficalho &amp;amp; Hiern) Stapf collection. The 20 available accessions are tetraploid (2n = 4x = 36). Chromosomes paired preferentially as bivalents, but quadrivalents were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brachiaria nigropedata; Chromosome number; Meiosis; Polyploidy; Apomixis; Grasses.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300007

A male-sterile mutation in soybean (Glycine max) affecting chromosome arrangement in metaphase plate and cytokinesis
Provedor de dados: Biocell
Autores: Pires Bione,Nilton Cesar; Suely Pagliarini,Maria; Alves de Almeida,Leones.
A spontaneous male-sterile, female-fertile mutation affecting bivalent arrangement at the metaphase plate and cytokinesis was detected in line BR98-197 of the soybean breeding program developed by Embrapa - National Soybean Research Centre. Untill diakinesis, meiosis was normal with chromosome pairing as bivalents. From this phase, in several meiocytes, bivalents were not able to organize a single metaphase plate and remained scattered in the cytoplasm in a few or several groups. In these meiocytes, chromosomes segregated in both divisions giving rise to several micronuclei. However, the main cause of male sterility was the absence of cytokinesis after telophase II. Instead of the typical tetrads of microspores, four nucleate coenocytic microspores were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Meiosis; Metaphase plate; Cytokinesis; Male sterility.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200004

C2- and C4-position 17&amp;#946;-estradiol metabolites and their relation to breast cancer
Provedor de dados: Biocell
Autores: Joubert,Annie; Van Zyl,Hermia; Laurens,Johannes; Lottering,Mona-Liza.
C2- and C4-position 17&amp;#946;-estradiol metabolites play an important role in breast carcinogenesis. 2-Hydroxyestradiol and 4-hydroxyestradiol are implicated in tumorigenesis via two pathways. These pathways entail increased cell proliferation and the formation of reactive oxygen species that trigger an increase in the likelihood of deoxyribonucleic acid mutations. 2-Methoxyestradiol, a 17&amp;#946;-estradiol metabolite, however, causes induction of apoptosis in transformed and tumor cells; thus exhibiting an antiproliferative effect on tumor growth. The 4-hydroxyestradiol:2-methoxyestradiol and 2-hydroxyestradiol:2-methoxyestradiol ratios therefore ought to be taken into account as possible indicators of carcinogenesis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Estradiol metabolism; 2-methoxyestradiol.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000300001

Ultrastructural analysis and identification of membrane proteins in the free-living amoeba Difflugia corona
Provedor de dados: Biocell
Autores: Silva-Briano,Marcelo; Martínez-Hernández,Sandra Luz; Adabache-Ortíz,Araceli; Ventura-Juárez,Javier; Salinas,Eva; Quintanar,J. Luis.
Syntaxin-1 and 25-kDa Synaptosome-associated Protein (SNAP-25) are present in the plasma membrane of several different secretory cell types and are involved in the exocytosis process. In this work, the free-living amoeba Difflugia corona was studied in relation to ultrastructure, structural membrane proteins, and proteins such as Syntaxin-1 and SNAP-25. Our results obtained by scanning electron microscopy in the amoeba without its theca, showed many membrane projections and several pore-like structures. Using immunocytochemistry, we found structural proteins Syntaxin-1 and SNAP-25.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amoeba; Membrane proteins; Pore-like; Theca; Ultrastructure.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000200004

Biochemical composition, tissue origin and functional properties of egg perivitellins from Pomacea canaliculata
Provedor de dados: Biocell
Autores: Dreon,Marcos S.; Heras,Horacio; Pollero,Ricardo J.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apple snails; Lipoproteins; Glycoproteins; Carotenoproteins; Embryo development; Antioxidant compounds; Light-protecting compounds.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200013

Ultrastructure of the Lyonet's glands in larvae of Diatraea saccharalis Fabricius (Lepidoptera: Pyralidae)
Provedor de dados: Biocell
Autores: Victoriano,Eliane; Gregório,Elisa A.
The Lyonet's gland is found in Lepidoptera larvae, close to the excretory duct of the silk gland. The role played by this gland is still uncertain. This work aims to describe the ultrastructure of the Lyonet's gland in Diatraea saccharalis larvae, offering suggestions regarding its possible function. The insects were reared under laboratory-controlled conditions. The glands were conventionally prepared for transmission (TEM) and scanning (SEM) electron microscopy. SEM showed that Lyonet's glands are paired small structures located in the ventral side of the head. They are composed by clustered long cells resembling leaves. Under TEM observations, each cell is surrounded by a thin basal lamina and contains large stellate nucleus. The cytoplasm presents...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Accessory gland; Insect; Sugarcane borer; Morphology.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000200006

Morphoquantitative evaluation of the duodenal myenteric neuronal population in rats fed with hypoproteic ration
Provedor de dados: Biocell
Autores: Marçal Natali,María Raquel; Molinari,Sonia Lucy; Valentini,Luiz Cristiano; Hubner de Miranda Neto,Marcílio.
The purpose of this work was to analyze the morphoquantitative behavior of the neurons of the myenteric plexus, as well as the morphometry of the duodenal wall, in adult rats fed with normoproteic (22%) and hypoproteic (8%) rations, killed at the age of 345 days. For neuronal assessments duodenal wholemounts stained with the Giemsa method were used, and for the evaluation of the duodenal wall routine histological processing and staining with Hematoxilin-Eosin were employed. The means of the number of neurons in 80 microscopic fields (12.72 mm2) and of the size of the neuronal cell bodies did not reveal statistically significant differences between the groups, but there was a greater incidence of large neurons in the protein restriction group (RP). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myenteric neurons; Small intestine; Morphometric; Hypoproteic ration.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000100006

Fossil record of Pomacea (Caenogastropoda: Ampullariidae) in Argentina and its paleoenvironmental implications
Provedor de dados: Biocell
Autores: Martín,Pablo R.; de Francesco,Claudio G.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apple snails; Distribution; Paleontology; Paleoecology; Paleoclimate.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200011

Facultative and obligate symbiotic associations of Pomacea canaliculata (Caenogastropoda, Ampullariidae)
Provedor de dados: Biocell
Autores: Vega,I.A.; Damborenea,M.C.; Gamarra-Luques,C.; Koch,E.; Cueto,J.A.; Castro-Vazquez,A.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mollusk symbiosis; Applesnails; Endocytobiosis; Bacterial symbiosis; Eukaryotic symbiosis.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200014

Structural and ultrastructural characterization of zebu (Bosindicus) spermatozoa
Provedor de dados: Biocell
Autores: Aquino Luque,Maria Carolina; Báo,Sônia Nair.
The ultrastructure of normal, ejaculated spermatozoa of Bos indicus was studied by means of electron microscopy, being evaluated in two principal parts, the head and the tail. The head is flat, oval or paddle-shaped with a square base, which provides a concave recess for the insertion of the tail. The acrosome tightly covers the anterior two thirds of the nucleus. A distinct unilateral acrosomal bulge was observed along the apical edge of the head. The equatorial region demarcates the acrosome from the post-equatorial region that covers the caudal one third of the nucleus. The classical 9+9+2 fiber pattern which composes the axoneme was observed along three segments of the tail, namely middle, principal and terminal pieces. The axoneme is anteriorly bound...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spermatozoon; Ultrastructure; Bos indicus.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100005

Cyclic nucleotide phosphodiesterase inhibition increases tyrosine phosphorylation and hyper motility in normal and pathological human spermatozoa
Provedor de dados: Biocell
Autores: Yunes,Roberto; Fernández,Pedro; Doncel,Gustavo F.; Acosta,Aníbal A.
Our objective was to determine the effect of phosphodiesterase (PDE) inhibition on: 1) tyrosine phosphorylation of human spermatozoa at the tail level; and 2) sperm motion parameters and hyperactivated motility. The study was conducted with normozoospermic and asthenozoospermic samples incubated under in vitro capacitating conditions. The main outcome measures were computer-assisted sperm motion analysis and fluorescent immunodetection of phosphotyrosine-containing proteins. Pentoxifylline (PTX) was used as PDE inhibitor because of its wide use in the clinic. PTX-treatment significantly increased sperm velocity, hyperactivated motility and tyrosine-phosphorylation, both in normo and asthenozoospermic samples. Tyrosine-phosphorylation of tail proteins was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PDE inhibition; Phosphotyrosine proteins; Sperm hyperactivation; Sperm motility; Assisted reproduction.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300006

Isolation and molecular characterization of a cax gene from Capsella bursa-pastoris
Provedor de dados: Biocell
Autores: Lin,Juan; Zhang,Wen; Shi,Mingzhu; Wang,Xinglong; Sun,Xiaofen; Tang,Kexuan.
A new cation exchangers (CAXs) gene was cloned and characterized from Capsella bursapastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA sequence of cax from C. bursa-pastoris (designated as Cbcax51) was 1754 bp containing a 1398 bp open reading frame encoding a polypeptide of 466 amino-acid residues with a calculated molecular mass of 50.5 kDa and an isoelectric point of 5.69. The predicted CbCAX51 contained an IMP dehydrogenase/GMP reductase domain, two Na+/Ca2+ exchanger protein domains. Comparative and bioinformatics analyses revealed that CbCAX51 showed extensive homology with CAX from other plant species. The expression analysis by different treatments indicated that Cbcax51 could be activated by cold triggering and was related...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capsella bursa-pastoris; Cation exchangers (CAXs); RACE; Freezing tolerance.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000300003

Impact of pituitary FSH purification on in vitro early folliculogenesis in goats
Provedor de dados: Biocell
Autores: Magalhães,D.M.; Araújo,V.R.; Lima-Verde,I.B.; Matos,M.H.T.; Silva,R.C.; Lucci,C.M.; Báo,S.N.; Campello,C.C.; Figueiredo,J.R.
Porcine pituitary follicle stimulating hormone (pFSH) is known to regulate the production of growth factors that have an essential role in early foliculogenesis. However, the effects of different preparations of pFSH on the survival and development of caprine follicles are not yet known. The aim of this study was to evaluate the effects of different pFSH (Stimufol® and Folltropin®) on the in vitro survival and growth of caprine preantral follicles. Pieces of caprine ovarian tissues were cultured for either one or seven days in a supplemented Minimum Essential Medium, alone or containing either Stimufol® (50 ng/mL) or Folltropin® (10, 50, 100 and 1000 ng/mL). Fresh control ovarian tissues as well as cultured tissued were processed for histological and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Goat; Preantral follicles; FSH; Follicular growth.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200003

The importance of Arabidopsis seed mutants in the elucidation of the molecular basis of Endosperm Balance Number in tuberbearing Solanum species
Provedor de dados: Biocell
Autores: Cornejo,P.; Camadro,E.L.; Masuelli,R.W.
The Endosperm Balance Number (EBN) is an important concept for potato breeding and has evolutionary importance in tuber-bearing Solanum species. The EBN is part of the post-zygotic hybridization barriers in the group and represents a reproductive isolating mechanism. Few genes have been proposed to be involved in its genetic control; until now, however, neither specific genes nor its molecular basis have been well established. Histological observations of embryo and endosperm development in inter-EBN crosses in tuber-bearing Solanum revealed phenotypes similar to those recently described in Arabidopsis seed mutants. The common feature between them is that the endosperm nuclei become greatly enlarged and that embryos are arrested at the globular stage. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EBN; Solanum; Endosperm; Arabidopsis; Seed mutants.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000300001

Genotoxic effect of Physalis angulata L. (Solanaceae) extract on human lymphocytes treated in vitro
Provedor de dados: Biocell
Autores: Alves dos Santos,Raquel; Cabral,Teresinha Rosa; Cabral,Isabel Rosa; Greggi Antunes,Lusânia Maria; Pontes Andrade,Cristiane; Cerqueira dos Santos Cardoso,Plínio; de Oliveira Bahia,Marcelo; Pessoa,Claudia; Martins do Nascimento,José Luis; Rodríguez Burbano,Rommel; Satie Takahashi,Catarina.
Physalis angulata L (Solanaceae) is a medicinal plant from North of Brazil, whose different extracts and infusions are commonly used in the popular medicine for the treatment of malaria, asthma, hepatitis, dermatitis and rheumatism. However, the genotoxic effects of P. angulata on human cells is not well known. The main purpose of the present study was to evaluate the in vitro genotoxic effects of aqueous extract of P. angulata using the comet assay and the micronucleus assay in human lymphocytes provided from 6 healthy donors. Treatments with P. angulata extracts were performed in vitro in order to access the extent of DNA damage. The comet assay has shown that treatments with P. angulata at 0.5, 1.0, 2.0, 3.0 and 6.0 &amp;#956;g/mL in culture medium were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P. angulata; Micronuclei; Comet assay.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200005

Interaction of bifidobacteria with the gut and their influence in the immune function
Provedor de dados: Biocell
Autores: Perdigón,Gabriela; Locascio,Mónica; Medici,Marta; Pesce de Ruiz Holgado,Aida; Oliver,Guillermo.
Bifidobacteria are predominant in the lumen of the large intestine and confer various health benefits on the host. They are also used in the preparation of new fermented milks (bioyogurts) or added to conventional yogurt to generate probiotic effects. The colonization of the gut by bacteria tends to be host specific due partly to the way in which bacteria adhere to the intestinal wall. Using a homologous strain of Bifidobacterium animalis in an experimental mouse model, we analyzed by immunofluorescence labelled-bacteria and transmission electronic microscopy the importance of this bacterial interaction with epithelial an immune cells associated to the gut, and the effect of feeding of B. animalis in the immune response. It was able to adhere and interact...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bifidobacteria; Homologous and Heterologous strains; Immune System.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000100001

Comparative fine structure of the epididymal spermatozoa from three korean shrews with considerations on their phylogenetic relationships
Provedor de dados: Biocell
Autores: Soon-Jeong,Jeong; Joo-Cheol,Park; Heung-Joong,Kim; Chun,Sik Bae; Myung-Hee,Yoon; Do-Seon,Lim; Moon-Jin,Jeong.
This study examined the fine structures of epididymal spermatozoa on the lesser white-toothed shrew ( Crocidura suaveolens ), the Japanese white-toothed shrew ( C. dsinezumi ) and the big white-toothed shrew ( C. lasiura ) belonging to the subfamily Crocidurinae living in Korea . In the spermatozoa of C. suaveolens , the head has a large acrosome, a smooth inner acrosomal membrane and a wavy, finger-like, electron-dense apical body. The neck has a solid proximal centriole that is filled with electron-dense material. These results showed the spermatozoa of C. suaveolens possess the characteristics of both Crocidurinae and Soricinae. In C. dsinezumi and C. lasiura , the head has a large acrosome, a serrated inner acrosomal membrane and a common apical body....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crocidurinae; Shrew; Spermatozoa; Phylogeny; Fine structure.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200003

Variation in worm assemblages associated with Pomacea canaliculata (Caenogastropoda, Ampullariidae) in sites near the Río de la Plata estuary, Argentina
Provedor de dados: Biocell
Autores: Damborenea,C.; Brusa,F.; Paola,A.
Pomacea canaliculata is a common gastropod in freshwater habitats from Central and Northern Argentina, extending northwards into the Amazon basin. Several Platyhelminthes have been reported associated to P. canaliculata, sharing an intimate relationship with this gastropod host. The objectives of this study were to describe the symbiotic species assemblages associated to P. canaliculata in the study area, and to disclose differences among them. Samples were taken in three typical small streams and one artificial lentic lagoon, all connected with the Río de la Plata estuary. The 81.53% were infested with different symbiotic (sensu lato) species. Among the Platyhelminthes, the commensal Temnocephala iheringi Haswell, 1893 was highly prevalent in all samples,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apple-snails; Platyhelminthes; Trematoda; Annelida; Hirudinea; Symbiosis; Commensalism; Parasitism.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000300006

Agmatine pretreatment protects retinal ganglion cells (RGC-5 cell line) from oxidative stress in vitro
Provedor de dados: Biocell
Autores: Yoko,Iizuka; Hong,Samin; Yun Kim,Chan; Kab Kim,Seung; Je Seong,Gong.
Agmatine, 2-(4-aminobutyl)guanidine, has been reported to have neuroprotective effects against various neuronal damages. In this study it was investigated whether agmatine pretreatment rescues the retinal ganglion cells from oxidative injury in vitro. After differentiation of transformed rat retinal ganglion cells (RGC-5 cell line) with staurosporine, agmatine (0.0 to 100.0 &amp;#956;M) pretreatment was performed for 2 hours. Subsequently, they were exposed to hydrogen peroxide (0.0 to 2.5 mM) as an oxidative stress. Cell viability was monitored for up to 48 hours with the lactate dehydrogenase (LDH) assay and apoptosis was examined by the terminal deoxynucleotide transferase-mediated terminal uridine deoxynucleotidyl transferase nick end-labeling (TUNEL)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuronal damage; Apoptosis; Neuroprotection.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000300005

Antibacterial activity of lactose-binding lectins from Bufo arenarum skin
Provedor de dados: Biocell
Autores: Sánchez Riera,Alicia; Daud,Adriana; Gallo,Adriana; Genta,Susana; Aybar,Manuel; Sánchez,Sara.
Amphibians respond to microbial infection through cellular and humoral defense mechanisms such as antimicrobial protein secretion. Most humoral defense proteins are synthetized in the skin. In this study we isolated two b-galactoside-binding lectins with molecular weights of 50 and 56 KDa from the skin of Bufo arenarum. These lectins have significant hemagglutination activity against trypsinized rabbit erythrocytes, which was inhibited by galactose-containing saccharides. They are water-soluble and independent of the presence of calcium. The antimicrobial analysis for each lectin was performed. At mmolar concentration lectins show strong bacteriostatic activity against Gram negative bacteria (Escherichia coli K12 4100 and wild strains of Escherichia coli...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Skin; Lectin; Amphibian; Antibacterial activity.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000100005

Decrease of intestinal tumors induced by 1,2-dimethylhydrazine in rats fed with cow milk and buffalo milk
Provedor de dados: Biocell
Autores: Sánchez Negrette,M; Montenegro,M.A.; Catuogno,M.S.; Lértora,W.J.; Guanziroli,M.C.
Epidemiological studies in human beings and experimental studies in laboratory animals suggest that milk and dairy products can inhibit effects on the development of some kinds of tumors. Cow milk contains sphingomyelin, butyric acid, conjugated linoleic acid, calcium, vitamin A, carotene and vitamin D. All of these components are known to inhibit the process of carcinogenesis. Our objective was to determine the effect of cow milk and water buffalo milk on the development of colon neoplasias in an experimental model of carcinogenesis in rats induced with 1,2-dimethylhydrazine (DMH). Three-month-old Wistar male rats with an average body weight of 180 g were given a nutritionally adequate diet and drinking water adlivitum, cow milk or water buffalo milk. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal cancer; Milk; Cow milk; Buffalo milk; DMH; Rats.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300005

Effects of desiccation on Euterpe edulis Martius seeds
Provedor de dados: Biocell
Autores: Panza,Víctor; Láinez,Verónica; Maldonado,Sara; Maroder,Horacio L.
Information on desiccation sensitivity of Euterpe edulis seeds under two drying rates is presented. The sensitivity was studied during the course of germination and normal germination. The water content was evaluated for both seeds and embryos. Results showed the following: (a) For both drying treatments and for both germination and normal germination, desiccation sensitivity values were higher for measurements based on the water content of the embryo than for those of the seed. (b) For both drying treatments, desiccation sensitivity were higher for normal germination than for germination based on both the embryo and seed water contents. (c) Under the slow drying treatment and for measurements based on the seed water content, critical water content was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drying chamber dehydration; Embryo desiccation sensitivity; Euterpe edulis seed; Silica gel drying; Recalcitrant seed.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300004

A 66-kDa protein of bovine hypophyseal Pars tuberalis induces luteinizing hormone release from rat Pars distalis
Provedor de dados: Biocell
Autores: Lafarque,Martha; Oliveros,Liliana.
In this study, evidence for a factor secreted by bovine hypophyseal pars tuberalis that stimulates luteinizing hormone (LH) release from rat pars distalis cells is shown. The secretion products of bovine pars tuberalis cells into the culture medium were assayed on dispersed rat pars distalis cells in 30 min incubations and superfusion experiments. The culture medium from pars tuberalis total cell populations, added at a dose of 6 &amp;#956;g per tube, induced the greater LH release from pars distalis cells, without effect on follicle stimulating hormone (FSH) release. After pars tuberalis cells separation on a discontinuos Percoll gradient, only the culture medium of cells from 50 and 60% strength Percoll were able to release LH from rat pars distalis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adenohypophysis; Gonadotropes; Lactotrophs; Follicle stimulating hormone; Prolactin.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000300001

Optimization and comparison of two different 3D culture methods to prepare cell aggregates as a bioink for organ printing
Provedor de dados: Biocell
Autores: Imani,Rana; Hojjati Emami,Shahriar; Fakhrzadeh,Hossein; Baheiraei,Nafiseh; Sharifi,Ali M.
The ultimate goal of tissue engineering is to design and fabricate functional human tissues that are similar to natural cells and are capable of regeneration. Preparation of cell aggregates is one of the important steps in 3D tissue engineering technology, particularly in organ printing. Two simple methods, hanging drop (HD) and conical tube (CT) were utilized to prepare cell aggregates. The size and viability of the aggregates obtained at different initial cell densities and pre-culture duration were compared. The proliferative ability of the cell aggregates and their ability to spread in culture plates were also investigated. In both methods, the optimum average size of the aggregates was less than 500 µm. CT aggregates were smaller than HD aggregates....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue engineering; Hanging drop; Conical tube; Bioprinting.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000100003

Herbicide effects on cuticle ultrastructure in Eleusine indica and Portulaca oleracea
Provedor de dados: Biocell
Autores: Malpassi,Rosana N.
Eleusine indica and Portulaca oleracea are two common weeds in peanut crops in southern CÃ³rdoba. Two chemicals are frequently used to control them, quizalofop for grasses and lactofen for dicots. The objective is to study the effects of quizalofop and lactofen on cuticle ultrastructure in E. indica and P. oleracea, respectively. In the lab, quizalofop was applied on E. indica and lactofen on P. oleracea. Three plant categories were analyzed in each species: 3, 1-2, and no tiller in E. indica, and 8, 6, and 2 nomophylls in P. oleracea. Leaf samples from both species were collected at 7 and 16 days post-application and were treated for scanning electron microscopy. E. indica cuticle treated with lethal dose shows areas where epicuticular waxes disappear,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quizalofop; Lactofen; Cuticle; E. indica; P. oleracea.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100008

Cobalt chloride stimulates phosphoinositide 3-kinase/Akt signaling through the epidermal growth factor receptor in oral squamous cell carcinoma
Provedor de dados: Biocell
Autores: Heon Ryu,Mi; Hee Park,Jeong; Eun Park,Ji; Chung,Jin; Hun Lee,Chang; Ryoun Park,Hae.
Tumor cells are often found under hypoxic conditions due to the rapid outgrowth of their vascular supply, and, in order to survive hypoxia, these cells induce numerous signaling factors. Akt is an important kinase in cell survival, and its activity is regulated by the upstream phosphoinositide 3-kinase (PI3K) and receptor tyrosine kinases (RTKs). In this study, we examined Akt activation and RTKs/PI3K/Akt signaling using the hypoxia-mimetic cobalt chloride in oral squamous carcinoma cells. Cobalt chloride increases Akt phosphorylation in both a dose- and time-dependent manner. Blocking the activation of the PI3K/Akt pathway using LY294002 abolished Akt activation in response to cobalt chloride, suggesting that Akt phosphorylation by cobalt chloride is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypoxia; DNA synthesis; S-phase.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000100003

Micropropagation of Glandularia perakii Cov. et Schn. (Verbenaceae): a native species with ornamental potential
Provedor de dados: Biocell
Autores: Marino,Concepción; Ponce,María T.; Videla,María E.; Fioretti,Sonia; Cirrincione,Miguel.
Glandularia perakii is a perennial species with beautiful violet flowers that grows in the stony soil of Mendocine pedemont. A plentiful and prolonged flowering confers it an important ornamental potential. In this paper, a method of propagation of G. perakii from nodal segments is reported. Proliferating microshoot cultures were obtained by placing nodal segment on Murashige and Skoog medium (MS) supplemented with 20 g.L-1 of sucrose without growth regulators. In this medium multiplication rate after 20 days was 7.9. Rooted plants were acclimatized successfully.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tissue culture; In vitro propagation; Nodal segment; Glandularia perakii.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000100007

Ultrastructural characteristics of the lung of Melanophryniscus stelzneri stelzneri (Weyenberg, 1875) (Anura, Bufonidae)
Provedor de dados: Biocell
Autores: Hermida,Gladys N.; Farías,Alejandro; Fiorito,Luisa E.
The lung of the toad, Melanophryniscus stelzneri stelzneri was studied using scanning and transmission electron microscopy. In M.s.stelzneri the parenchyma forms a polygonal network arrangement, therefore the parenchyma is edicular. These spaces are delimited by the interconnection of third order septa which are covered by respiratory epithelium. Small patches of ciliated epithelium without goblet cells appear irregularly distributed on the septa. The respiratory epithelium consists of one type of pneumocyte, which shows characteristics of both type I and type II alveolar cells of higher vertebrates. The pneumocytes are irregular in shape and possess attenuated cytoplasmic processes, which spread around the capillaries to form the outer layer of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung ultrastructure; Respiratory epithelium; Electronic microscopic; Toad; Amphibian.
Ano: 2002
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452002000300005

Microcutting culture and morpho-physiological changes during acclimation in two Lycium chilense cytotypes
Provedor de dados: Biocell
Autores: Maseda,Pablo Horacio; Lemcoff,Jorge Hugo; Murúa,Mercedes; Frayssinet,Nora; Carceller,Marta Susana.
Lycium chilense, a deciduous perennial shrub, is one of the endangered native species of Patagonia due to sheep overgrazing. Chances of recolonization by seeds are scarce due to the limited density of propagules in the soil and very specific requirements for germination. The objective was to develop an in vitro propagation protocol that would help to perform reestablishment of this species in degraded areas of the Patagonian steppe. Seeds came from two provenances with different somatic number due to differences in ploidy level. Defoliated microcuttings were planted in test tubes with different growing media and taken to a growth chamber. Rooting percentage did not differ between origins, but higher values were encountered for medium without hormones....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microcuttings; Lycium chilense; Subcultures; Endangered species.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000300003

Cytochemical localization of acid phosphatase in Stigeoclonium tenue (Chaetophorales, Chlorophyceae)
Provedor de dados: Biocell
Autores: Michetti,Karina M.; Leonardi,Patricia I.; Cáceres,Eduardo J.
Nonspecific acid phosphatases are a group of enzymes whose activity increases the availability of exogenous and endogenous orthophosphate either through extra- or intracellular hydrolysis of phosphate compounds. Our study demonstrates the activity of acid phosphatases in the filamentous freshwater alga Stigeoclonium tenue. These enzymes were detected following a cerium-based method in which cerium was used as an orthophosphate-capture reagent. In thalli from S. tenue from the natural environment, acid phosphatases were found in the longitudinal cell wall, plasmalemma, and vacuole. In thalli from Bold's Basal Medium culture, these enzymes were found mainly in the plasmalemma; they were scarce in the cell wall. In the thalli grown in phosphate-enriched...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chaetophorales; Acid phosphatase; Stigeoclonium tenue; Ultrastructure.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000300009

Dra. Haydée Judith Viloria de Castejón (1938-2005)
Provedor de dados: Biocell
Autores: Castejón,Orlando J.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300010

Expression of caveolin-1 in rat Leydig cells
Provedor de dados: Biocell
Autores: Casanova,Marta B.
Caveolin-1, the first member of caveolin family reported, is recognized as the structural component of caveola, a plasma membrane invagination or vesicles that are a subcompartment distinct from clathrin-coated pits. This protein is also known to be involved in cholesterol trafficking. The aim of this study was to determine the expression of caveolin-1 in adult rat Leydig cells. Testis sections incubated with an antibody to caveolin-1 showed, by immunohistochemistry, a moderate number of Leydig cells with different degrees of immunoreaction and a strong reaction in endothelial cells and in the lamina propia of seminiferous tubules. Caveolin-1 was detected in the cell cytoplasm with a granular pattern and on the cell surface of Leydig cells cultured 24 h on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Testis; Leydig cells; Caveolin-1.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000300003

Argentinean cultivars of Vitis vinifera grow better than European ones when cultured in vitro under salinity
Provedor de dados: Biocell
Autores: Cavagnaro,Juan B.; Ponce,María T.; Guzmán,Javier; Cirrincione,Miguel A.
Argentinean Vitis vinifera cultivars although originated from Europe, have clear ampelographic and genotypic differences as compared with the European cultivars currently used in wine making. In vitro evaluation of salt tolerance has been used in many species. Our hypothesis was that Argentinean cultivars are more tolerant to salinity than European ones. Three European cultivars, Malbec, Cabernet Sauvignon and Chardonnay and four Argentinean cultivars, Cereza, Criolla Chica, Pedro Gimenez and Torrontes Riojano were tested by in vitro culture. Treatments included: 1) Control, 2) 60 mEq/L of a mixture of three parts of NaCl and one part of CaCl2 and 3) 90 mEq/L of the salt mixture. Results from two experiments (I and II) are reported. No differences were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitis vinifera; Cultivars; Salt tolerance; In vitro; G rowth.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100001

Effects of high molybdenum intake on 1,2-dimethylhydrazine-induced intestinal tumors in rats
Provedor de dados: Biocell
Autores: Montenegro,M.A.; Sánchez Negrette,M.; Gimeno,E.J.; Borda,J.T.
Wistar male rats, 3 months of age were given ad-libitum a nutritionally adequate diet and demineralized drinking water. The Molybdenum (Mo) and Tungsten (W) were provided in the drinking water at 200 ppm concentration. Intestinal tumors were induced by 1,2-dimethylhydrazine (DMH) given subcuta-neously as 16 weekly doses at 20 mg/kg body weight. Mo in the form of (NH 4 ) 6 Mo 7 O 24 4H 2 O or W in the form of (Na 2 WO 4 ) were provided in the drinking water two months before the first DMH treatment and were continued during 4 months more until the last DMH treatment. Three months after the last carcinogen injec-tion, all animals were sacrificed and examined for intestinal tumors. The number, size and location of the tumors were recorded and the pathology...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal cancer Molybdenum 1; 2-dimethylhydrazine Rats.
Ano: 2002
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452002000300004

Abstracts from the XXVII Annual Scientific Meeting December 11-13, 2009 Ciudad de la Punta San Luis-Argentina
Provedor de dados: Biocell
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000100009

Age related interaction of dopamine and serotonin synthesis in striatal synaptosomes
Provedor de dados: Biocell
Autores: Messripour,Manoochehr; Mesripour,Azadeh.
Tyrosine hydroxylase and tryptophan hydroxylase are key rate limiting enzymes in the biosyn-thesis of dopamine and serotonin, respectively. Since both enzymes are active in striatum, and affected by age, this study was undertaken to investigate interaction between dopamine and serotonin synthesis in brain striatal synaptosomes of aging rat. Male Wistar rats (3 and 30 month old) were killed by decapitation and brain striatal synaptosomes were prepared by discontinuous Ficoll/sucrose gradient technique. Synaptosomes were incubated in the presence of added pargiline (monoamineoxidase inhibitor), dopamine or serotonin synthesized during 25 min was measured by HPLC, employing electrochemical detection. Dopamine synthesis in synaptosomes prepared from young...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Nerve ending; Neurotransmitter; Tyrosine hydroxylase; Tryptophan hydroxylase.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000200001

An ultrastructural study of spermiogenesis in two species of Sitophilus: Coleoptera: Curculionidae
Provedor de dados: Biocell
Autores: Name,Khesller Patrícia Olázia; Dos Reis,Giuliano Pagy Felipe; Báo,Sônia Nair.
The spermiogenesis of Sitophilus zeamais and Sitophilus oryzae, the maize and the rice weevil, respectively, was studied by light microscopy and scanning and transmission electron microscopy. Sitophilus spp. is the most widespread and destructive primary pest of stored cereals in the world. The spermiogenesis occurs within cysts. There are approximately 256 germ line cells per cyst. Inside each cysts, all the spermatids are in the same stage of maturation. The ultrastructure of the spermatozoa of S. zeamais and S. oryzae is similar to that described for other beetles. The head is formed by a three-layered acrosome with the perforatorium, the acrosomal vesicle, the extra-acrosomal layer and the nucleus. The flagellum has the typical axoneme formed by a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curculionidae; Sitophilus; Spermiogenesis; Insect sperm; Ultrastructure.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000200005

Comparison of inflammatory microRNA expression in healthy and periodontitis tissues
Provedor de dados: Biocell
Autores: Wa Lee,Young H; Am Na,Hee S; Jeong,So Y Eon; Jeong,Sung H Ee; Park,Hae R Youn; Chung,Jin.
MicroRNAs (miRNAs) are short RNA molecules that negatively regulate gene expression primarily by degrading target mRNA or inhibit the translation of protein product. Recently, many reports have shown the altered miRNA expression in various diseases. However, there are no reports on miRNA expression related to periodontitis. Thus, this study aimed to compare the miRNAs differentially expressed in healthy and chronic periodontitis tissues and to determine the miRNAs closely associated with chronic periodontitis. To find out the miRNAs differentially induced in healthy and chronic periodontitis tissues, miRNA microarray was carried out and the expression of miRNAs was confirmed by real-time PCR. According to miRNA microarray analyses, six miRNA genes, let-7a,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microarray; Real-time PCR; Gingiva.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000200002

Abstracts from the XXVI Annual Scientific Meeting: October 14 - 16, 2009 Tafí del Valle, Tucumán, Argentina
Provedor de dados: Biocell
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000200007

Butein imparts free radical scavenging, anti-oxidative and pro-apoptotic properties in the flower extracts of Butea monosperma
Provedor de dados: Biocell
Autores: Sehrawat,Anuradha; Kumar,Vijay.
The flower of Butea monosperma (Lam.) (Fabaceae) has been used in traditional Indian medicine in the treatment of many ailments including liver disorders. To understand the pharmacological basis of its beneficial effects, the extracts of dried flowers in water, methanol, butanol, ethyl acetate and acetone were evaluated for free radical scavenging and pro-apoptotic activities in cell cultures (human hepatoma Huh-7 cell line and immortalized AML-12 mouse hepatocytes). Butrin and butein -the active constituents of flower extracts- were used as reference molecules. The levels of cell injury markers like lactate dehydrogenase, glutathione and lipid peroxidation and primary antioxidant enzymes glutathione S-transferase and catalase were also measured. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Butrin; Cytotoxicity; Hepatoprotection; Methanol extract; Free radical scavenging.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000200003

Seasonal variations in the heterologous binding of viscacha spermatozoa: A scanning electron microscope study
Provedor de dados: Biocell
Autores: Aguilera Merlo,Claudia; Muñoz,Estela; Dominguez,Susana; Fóscolo,Mabel; Scardapane,Luis; de Rosas,Juan Carlos.
Seasonal changes in the reproductive activity of the adult male viscacha (Lagostomus maximus maximus) were investigated during the annual reproductive cycle. Assays of heterologous in vitro binding between compatible gametes were used to evaluate the ability of viscacha spermatozoa to achieve primary binding during its annual reproductive cycle. Sperm were collected by mincing cauda epididymis in HECM-3 medium and the sperm concentration and motility were evaluated. Cumulus-free and zona-free oocytes obtained from superovulated hamsters were inseminated in vitro with capacitated sperm suspensions, incubated at 37ºC, 5% CO2 for 3 h, and then processed for studies by scanning electronic microscopy. Statistical analysis was used to compare the quantitative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Viscacha (Lagostomus maximus maximus); Spermatozoa; Heterologous binding; Seasonal variations.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300001

Effects of rotational culture on morphology, nitric oxide production and cell cycle of endothelial cells
Provedor de dados: Biocell
Autores: Chaojun,Tang; Wu,Xue; Linqi,Ye; Xiang,Xie; Guixue,Wang.
Devices for the rotational culture of cells and the study of biological reactions have been widely applied in tissue engineering. However, there are few reports exploring the effects of rotational culture on cell morphology, nitric oxide (NO) production, and cell cycle of the endothelial cells from human umbilical vein on the stent surface. This study focuses on these parameters after the cells are seeded on the stents. Results showed that covering of stents by endothelial cells was improved by rotational culture. NO production decreased within 24 h in both rotational and static culture groups. In addition, rotational culture significantly increased NO production by 37.9% at 36 h and 28.9% at 48 h compared with static culture. Flow cytometry showed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endothelial cells; Rotational culture; Stents; Nitric oxide.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300001

DNA mismatch repair gene methylation in gastric cancer in individuals from northern Brazil
Provedor de dados: Biocell
Autores: Moura Lima,Eleonidas; Ferreira Leal,Mariana; de Arruda Cardoso Smith,Marília; Rodríguez Burbano,Rommel; Pimentel de Assumpção,Paulo; Bello,Maria Jose; Rey,Juan Antonio; Ferreira de Lima,Francinaldo; Casartelli,Cacilda.
Gastric cancer is one of the most common malignancies. DNA methylation is implicated in DNA mismatch repair genes deficiency. In the present study, we evaluated the methylation status of MLH1, MSH2, MSH6 and PMS2 in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosal of gastric cancer patients from Northern Brazil. We found that none of the nonneoplastic samples showed methylation of any gene promoter and 50% of gastric cancer samples showed at least one methylated gene promoter. Methylation frequencies of MLH1, MSH2, MSH6 and PMS2 promoter were 21.74%, 17.39%, 0% and 28.26% respectively in gastric cancer samples. MLH1 and PMS2 methylation were associated with neoplastic samples compared to nonneoplastic ones. PMS2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric cancer; MLH1; MSH2; MSH6; PMS2.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000300004

Cytogenetical and ultrastructural effects of copper on root meristem cells of Allium sativum L.
Provedor de dados: Biocell
Autores: Liu,Donghua; Jiang,Wusheng; Meng,Qingmin; Zou,Jin; Gu,Jiegang; Zeng,Muai.
Different copper concentrations, as well as different exposure times, were applied to investigate both cytogenetical and ultrastructural alterations in garlic (Allium sativum L.) meristem cells. Results showed that the mitotic index decreased progressively when either copper concentration or exposure time increased. C-mitosis, anaphase bridges, chromosome stickiness and broken nuclei were observed in the copper treated root tip cells. Some particulates containing the argyrophilic NOR-associated proteins were distributed in the nucleus of the root-tip cells and the amount of this particulate material progressively increased with increasing exposure time. Finally, the nucleolar material was extruded from the nucleus into the cytoplasm. Also, increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Garlic; Organelle; Cytogenetical and ultrastructural alterations; Mitosis.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000100003

Abstracts from the XI CONGRESS - XXIX ANNUAL MEETING December, 3 and 4, 2009
Provedor de dados: Biocell
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000100008

Organogenesis and plant regeneration of Arachis villosa Benth. (Leguminosae) through leaf culture
Provedor de dados: Biocell
Autores: Fontana,María Laura; Mroginski,Luis Amado; Rey,Hebe Yolanda.
With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: &amp;#945;-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 &amp;#956;M thidiazuron and 4.44 &amp;#956;M 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in Arachis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro cultivation; Leaflet explants; Micropropagation; Thidiazuron; Wild groundnut.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000300006

Fine structural study of the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta)
Provedor de dados: Biocell
Autores: Estevez,José M.; Cáceres,Eduardo J.
The present study analyzed several characters of the red seaweed Gymnogongrus torulosus, such as cellular structure of the thallus, cuticle, pit plug and cell wall ultrastructure, and morphology of some organelles like plastids, Golgi bodies and mitochondria. Also, anomalous chloroplasts with thylakoid disorganization were found in medullary cells. The significance of this thylakoid disposition is still unclear. This is one of the first studies focused on the fine structure of a red alga recorded in Argentina.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anomalous Chloroplasts; Cell Wall; Cystocarpic Thalli; Fine Structure; Gymnogongrus Torulosus; Pit Plugs; Rhodophyta.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000200003

Determination of vanadium accumulation in onion root cells (Allium cep a L.) and its correlation with toxicity
Provedor de dados: Biocell
Autores: Marcano,Letty; Carruyo,Ingrid; Fernández,Yusmary; Montiel,Xiomara; Torrealba,Zaida.
The vanadium is a metal that presents great interest from the toxicological point of view, because of the numerous alterations that can take place in different biological systems. This work evaluated the capacity of vanadium accumulation and its correlation with genotoxic effects in root cells of Allium cepa L. The bulbs were cultivated in renovated filtered water each 24 h, at a temperature of 25 ± 0.5 ° C, in darkness and constant aeration. Treatments were carried out under the same experimental conditions, using water solutions of vanadium of 25, 50, 75 and 100 µ g/g for 0, 12, 24, 48 and 72 h. A control was carried out where metal solution was substituted by distilled water. After the treatment, the meristems were fixed with alcohol - acetic acid (3:1)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Root cells; Vanadium; Toxicity; Accumulation; GFAAS.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200001

Isolation of stem cells from adult rat kidneys
Provedor de dados: Biocell
Autores: Gheisari,Yousof; Soleimani,Masoud; Zeinali,Sirous; Arefian,Ehsan; Atashi,Amir; Nikougoftar Zarif,Mahin.
The kidney has an inherent ability for recovery and regeneration following acute damage. However, there has been much contention as to the source of regenerating renal cells. The aim of this study was to isolate and characterize these cells. Normal rat kidneys were minced and cells were isolated with collagenase I and were cultured in an expansion medium. Adherent cells were isolated and expanded for more than 120 days in vitro. These cells had the potential of translineage differentiation into neural cells, adipocytes and osteocytes. These cells also expressed Nucleostemin, Cyclin D1, Notch1 and Survivin which are commonly expressed in stem cells. The results of the current work show that the adult kidney contains a population of multipotent stem cells.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney failure; Regeneration; Cell differentiation; Renal replacement therapy.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000100004

In vitro effects of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and cell cycle dynamics in the MCF-7 breast adenocarcinoma cell line
Provedor de dados: Biocell
Autores: Vorster,Chris; Joubert,Annie.
In the search for new and improved anticancer therapies, researchers have identified several potentially useful compounds. One of these agents is 2-methoxyestradiol-bis-sulphamate (2ME-BM), a sulphamoylated derivative of 2-methoxyestradiol. The objective of this study was to evaluate 2ME-BM's in vitro efficacy as antiproliferative agent in the MCF-7 breast adenocarcinoma cell line. Light- and fluorescent microscopy showed decreased cell density, increased apoptotic characteristics and significant ultrastructural aberrations indicative of autophagic cell death after 24 hours of exposure at a concentration of 0.4µM. In addition, mitotic indices revealed that 2ME-BM induces a G2M block. The latter was confirmed by flow cytometric analyses where increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; 2-methoxyestradiol; Sulphamoylated estradiol derivative; Apoptosis; Autophagy.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000200003

Cell proliferation of the ileum intestinal mucosa of diabetic rats treated with ascorbic acid
Provedor de dados: Biocell
Autores: Nelisis Zanoni,Jacqueline; Fernandes Pereira,Renata Virginia.
The objective of this work was to evaluate the effect of the ascorbic acid supplementation on the cellular proliferation on the ileum mucosa of diabetic rats. Fifteen 90-days rats were divided in the groups: control, diabetic and diabetic supplemented with ascorbic acid (DA). Two hours prior the sacrifice, they were injected with Vincristin. Semi-seriate histological cuts stained with HE were accomplished. About 2500 crypt cells from the intestinal mucosa were counted in order to obtain the metaphasic indexes. The height and depth of 30 villi and 30 crypts were measured for each animal, respectively. The metaphasic indexes showed no significant changes when we compared the three groups: 20.2 ± 0.7 (control), 18 ± 1.9 (diabetic) and 17 ± 1.4 (DA) (p &gt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascorbic acid; Diabetes mellitus; Lleum; Cellular proliferation.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200001

Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes
Provedor de dados: Biocell
Autores: Talia,Paola; Greizerstein,Eduardo J.; Hopp,H. Esteban; Paniego,Norma; Poggio,Lidia; Heinz,Ruth A.
Bacterial artificial chromosome - fluorescence in situ hybridization (BAC-FISH) and cycling-primed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. This selection was based on previously identified QTL associated to S. sclerotiorum. These markers were used to select BACs contaning single copy sequences for BAC-FISH aplication. Blocking of highly dispersed repetitive sunflower sequences reduced unspecific hybridization, and allowed the detection of specific signals for BACs containing SSR markers HA4222 and HA2600, anchored to LG 16 and LG 10, respectively. Single-site...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sunflower; BAC-FISH; C-PRINS; Single-copy sequences; Physical mapping.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000100003

Study of the myenteric and submucous plexuses after BAC treatment in the intestine of rats
Provedor de dados: Biocell
Autores: Buttow,Nilza Cristina; Santin,Miriam; Conci Macedo,Luciana; Neres Teixeira,Aline Cristina; Novakowski,Gisele Caroline; Bolonheis Armelin,Taíse Roberta; Assmann,Kathya.
A morphological and quantitative study in the ileal and colonic myenteric and submucous plexuses of rats after BAC denervation was performed. Four groups were employed: SI - ileum control; CBI - denervated ileum; SC - colon control; and CBC - denervated colon. We used the Myosin-V immunohistochemistry technique to study the myenteric and submucous plexuses. In the submucous plexus of the ileum and colon there was not a significant decrease in the number of neurons/mm2 and of ganglia/mm2. The denervation of the myenteric plexus in the group CBI was 44.7% and in the group CBC, 68.3%. In the myenteric plexus there was also a significant decrease in the number of ganglia/mm2 (13.8% in group CBI and 52.14% in group CBC) and in the number of neurons/ganglion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myenteric plexus; Submucous plexus; Morphology; Benzalkonium chloride; Denervation.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000200002

Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration
Provedor de dados: Biocell
Autores: Surenciski,Mauro Rodrigo; Flachsland,Eduardo Alberto; Terada,Graciela; Mroginski,Luis Amado; Rey,Hebe Yolanda.
The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30°C for 1 min, rehydrated using the same liquid mediums (0.75 M sucrose (24 h), 0.5 M...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryoconservation; Liquid nitrogen; Calcium alginate; Activated charcoal; Sucrose.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000100002

Hydrogen peroxide in micropropagation of Lilium: A comparison with a traditional methodology
Provedor de dados: Biocell
Autores: Curvetto,Néstor; Marinangeli,Pablo; Mockel,Gabriela.
The micropropagation of Lilium longiflorum requires adequate equipment which may not be afforded by small laboratories or producers. In this work we compared traditional methodology with a protocol that included easily available elements to sterilize materials and culture media, together with addition of hydrogen peroxide (H2O2) into the nutrient media as chemical sterilizer. A series of H2O2 concentrations (0.005, 0.010, 0.015 and 0.020% p/v) was used to control contamination during in vitro establishment and subsequent cultivation; the explant organogenic response was also examined and compared to the traditional micropropagation technique. The level of culture contamination was within acceptable limits in all treatments, though it was higher in the H2O2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemical sterilization; Lily; In vitro multiplication; Tissue culture.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000300010

Characterization of the Brain-pituitary axis in pejerrey Odontesthes bonariensis
Provedor de dados: Biocell
Autores: Somoza,G.M.; Miranda,L.A.; Guilgur,L.G.; Strobl-Mazzulla,P.H.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain; Pituitary; Reproduction; Sex differentiation.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100011

Genetic stability in rice micropropagation
Provedor de dados: Biocell
Autores: Medina,R; Faloci,M; Marassi,M.A.; Mroginski,L.A.
An efficient clonal propagation procedure for six rice varieties cultivated in Argentina was developed by using shoot tip cultures, and the genetic stability of the micropropagated plants was verified by isozyme analysis. One week old seedlings obtained on MS medium were sectioned and subcultured on MS medium (0.75% agar) supplemented with different combination and concentrations of cytokinins (BAP and KIN) and auxins (2,4-D and NAA). After four weeks of culture, multiple shoots were obtained. The best response was observed on MS supplemented with BAP 5 mg l-1. Shoot clumps were multiplied in MS liquid medium containing BAP 5 mg l-1. Profuse rooting was obtained after transfer to MS medium lacking growth regulators and with sucrose 8% (w/v). Complete...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Multiple shoots; Oryza sativa L.; Isozyme; Genetic stability.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000100002

Proteoglycans production by aortic vascular smooth muscle cells from hypertensive rats
Provedor de dados: Biocell
Autores: Risler,Norma; Castro,Claudia; Cruzado,Montserrat; González,Susana; Miatello,Roberto.
Remodeling of large and small arteries contributes to the development and complications of hypertension. Artery structural changes in chronic sustained hypertension include vascular smooth muscle cells (VSMC) proliferation and extracellular matrix (ECM) modifications. Extracellular constituents such as proteoglycans (PGs), may modulate vascular stiffness and VSMC growth and differentiation. We examined the effect of growth factors on secreted and membrane-bound PGs synthesis by cultured aortic smooth muscle cells (SMC) from 12- to 14- week-old spontaneously hypertensive rats (SHR) and age-matched Wistar rats. After stimulation with platelet-derived growth factor (PDGF-BB), 10% fetal calf serum (FCS) or 0.1% FCS as control, PGs synthesis (dpm/ng DNA) was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vascular Smooth Muscle Cells; Extracellular Matrix Proteoglycans; Hypertension; Spontaneously Hypertensive Rats.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000200004

In vitro propagation of Opuntia ellisiana Griff. and acclimatization to field conditions
Provedor de dados: Biocell
Autores: Juárez,María Cecilia; Passera,Carlos Bernardo.
The genus Opuntia is a valuable forage resource in arid and semiarid lands during periods of drought and shortage of herbaceous plants. However, absolute minimum temperatures in the plains of Mendoza represent a limiting factor to cultivate several species. Opuntia ellisiana is a cold hardy species, so the goals of this study were to massively propagate it using in vitro culture techniques, and then to acclimatize plantlets obtained to field conditions. Different sterilization protocols were tested. Areoles were isolated in laminar airflow cabinet, and cultured on Murashige-Skoog medium, supplemented with sucrose and different BAP and IBA combinations. Explants were grown at 27±2ºC, under a 16-h photoperiod. The shoots produced were used in the rooting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Areole culture; Cold hardy forage; Arid forage resources.
Ano: 2002
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452002000300002

Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells
Provedor de dados: Biocell
Autores: R odriguez,Andrea P.; Tsujigiwa,Hidetsugu; Gunduz,Mehmet; Cengiz,Beyhan; Nagai,Noriyuki; Tamamura,Ryo; Borkosky,Silvia S.; Takagi,Tohru; Inoue,Miho; Nagatsuka,Hitoshi.
Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed odontoblast-like characteristics but with a low ability to induce dentin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MDPC-23 cells; KUSA/A1 cells; Diffusion chamber; RT-PCR analysis; Immunohistochemical analysis.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000100005

Inhibitory effect of jasmonic acid and ethylene on epicotyl growth and bud induction in the maritime pine, Pinus pinaster Soland. in Ait
Provedor de dados: Biocell
Autores: Martin,Maria Teresa; Pedranzani,Hilda; García-Molinero,Patricia; Pando,Valentin; Sierra-de-Grado,Rosario.
Two independent parameters, epicotyl height (cm) and number of induced buds were studied on Pinus pinaster explants to analyse the effects of three phytohormones (6-benzylaminopurine, jasmonic acid, ethylene) which were combined or not in 11 different treatments. Epicotyle length diminished significantly in relation to the control medium (medium without exogen phytohormones) in presence of jasmonic acid, 6-benzylaminopurine or Ethephon (which is converted to ethylene in plants) in any of treatments. Concentrations of 100 &amp;#956;M of jasmonic acid and Ethephon had a greater inhibitory effect than the treatments with 10 &amp;#956;M. In addition to that, jasmonic acid was a stronger inhibitor than Ethephon in any of the tried combinations. There were no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Phytohormones; Plant growth regulators; 6-benzylaminopurine; In vitro plant culture.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000300002

NFAT regulates CSF-1 gene transcription triggered by L-selectin crosslinking
Provedor de dados: Biocell
Autores: Chen,Cuixia; Cui,Lingling; Shang,Xin; Zeng,Xianlu.
L-selectin is a member of the selectin family that play an important role both in mediating the initial capture and subsequent rolling of leukocytes along the endothelial cells. Furthermore, L-selectin can function as a signal molecule. In our previous studies, we reported that L-selectin ligation could regulate CSF-1 (colony-stimulating factor-1) gene transcription, in which AP-1 acts as a crucial transcriptional factor. Here we investigated the function of the NFAT in the CSF-1 gene transcriptional events. We found that overexpression of WT NFAT induce CSF-1 gene transcription greatly in the activated Jurkat cells. Furthermore, we found that NFAT can be recruited to the nucleus after L-selectin ligation, and the nuclear NFAT interacts with the CSF-1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Signal transduction; Gene transcription; Nuclear transcriptional factor.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000200001

Changes in crossover distribution along a quadrivalent in a man carrier of a reciprocal translocation t(11;14)
Provedor de dados: Biocell
Autores: Pigozzi,M.I.; Sciurano,R.B.; Solari,A.J.
A testicular biopsy from an infertile man carrying a heterozygous chromosome translocation t(11;14) was studied with synaptonemal complex analysis and immunolocalization of the protein MLH1 for crossover detection. A full blockage of spermatogenesis at the spermatocyte stage was related to the presence of the translocation quadrivalents at pachytene. Only 2% of the quadrivalents showed full synapsis. Most of the spermatocytes showed asynaptic free ends that frequently mingled with the XY pair. The average number of crossovers per cell was diminished from a mean of 52.7 in controls to a mean of 48 in the patient. The difference between the number of crossovers in the quadrivalent and the normal bivalents was highly significant. The distribution of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human meiotic recombination; Human meiosis; Crossovers; MLH1 foci; Chromosomal translocations.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200007

Defense reactions of Dermatobia hominis (Diptera: Cuterebridae) larval hemocytes
Provedor de dados: Biocell
Autores: Faraldo,Ana Carolina; Lello,Edy.
The defense reactions against biological (Histoplasma capsulatum and Escherichia coli) and non-biological materials (China ink and nylon thread) were tested in vivo in third instar larvae of Dermatobia hominis. The cellular defense performed by larval hemocytes was observed under electron microscopy. China ink particles were phagocytosed by granular cells 5 h after injection. E. coli cells were internalized by granular cells as early as 5 min after injection and totally cleared 180 min post-injection, when many hemocytes appeared disintegrated and others in process of recovering. H. capsulatum yeasts provoked, 24 h after being injected, the beginning of nodule formation. Nylon thread was encapsulated 24 h after the introduction into the hemocoel. Our...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dermatobia Hominis; Hemocytes; Phagocytosis; Nodule Formation; Encapsulation.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000200005

Morphologic and morphometric analysis of testis of Pseudis limellum (Cope, 1862) (Anura, Hylidae) during the reproductive cycle in the Pantanal, Brazil
Provedor de dados: Biocell
Autores: Ferreira,Adelina; Mehanna,Mahmoud; Prado,Cynthia P. A.
The spermatogenesis of Pseudis limellum, from the Southern Pantanal, Brazil, was studied from July 1995 to May 1996, through histological sections of the testis. The cells could be differentiated as: primary spermatogonia, large cells, generally with bilobed nucleus; secondary spermatogonia, smaller cells, with darker cytoplasm, chromatin of radial form; primary and secondary spermatocytes, differentiated according to the different stages of the nucleus during the successive cells divisions. Furthermore, we observed cells in process of morphologic differentiation: rounded spermatids much smaller, with nucleus containing chromatin in compacting process and cytoplasm reduction; elongated spermatids, generally parallel organized in well defined bundles, with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spermatogenesis; Male; Amphibia; Climatic influences.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200004

Cysteine proteinases of Trypanosoma cruzi: from digestive enzymes to programmed cell death
Provedor de dados: Biocell
Autores: Kosec,Gregor; Alvarez,Vanina; Cazzulo,Juan J.
Trypanosoma cruzi, the parasite causing Chagas disease, contains a number of proteolytic enzymes. The recent completion of the genome sequence of the T. cruzi CL Brener clone suggests the presence of 70 cysteine peptidases, 40 serine peptidases (none of them from the chymotrypsin family), about 250 metallopeptidases (most leishmanolysin homologues), 25 threonine peptidases, and only two aspartyl peptidases, none of them from the pepsin family. The cysteine peptidases belong to 7 families of Clan CA, 3 families of Clan CD, and one each of Clans CE and CF. In Clan CA, the C1 family is represented by cruzipains 1 and 2, biochemically well characterized, as well as cathepsin B and two other cathepsins. There are a number of homologues to calpains (family C2),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Chagas disease; Cysteine proteinases; Apoptosis; Autophagy.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000300008

Differences in intracellular localization of corn stunt spiroplasmas in magnesium treated maize
Provedor de dados: Biocell
Autores: Nome,Claudia; Magalhães,Paulo César; Oliveira,Elizabeth; Nome,Sergio; Laguna,Irma Graciela.
Maize plants infected with Spiroplasma kunkelii show symptoms similar to that of plants in a magnesium-deficient soil, and it has been shown that the spiroplasma alters the plants' magnesium absorption. In the current study we compared changes associated to either spiroplasma infection, two soil magnesium levels and their combinations. Plant symptoms were recorded and correlated with transmission electron microscopy observations. Plants grown on a high magnesium treatment showed no macroscopical alterations nor organelle ultrastructural alterations, while plants on a low magnesium treatment showed macroscopical vein yellowing and, ultrastructurally, they had most chloroplasts and mitochondrial membranes altered. Infected plants on a low magnesium treatment...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Mollicutes; Cytopathology; Plant pathogen; Magnesium deficiency.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200008

Ecotoxicological studies on the pejerrey (Odontesthes bonariensis, Pisces Atherinopsidae)
Provedor de dados: Biocell
Autores: Carriquiriborde,Pedro; Ronco,Alicia.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Odontesthes bonariensis; Ecotoxicology; Environmental pollution; Heavy metals; Pesticides.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100012

Comparative study of mandibular glands of Melipona bicolor queens obtained from polygynic and monogynic colonies
Provedor de dados: Biocell
Autores: Fioretti Gracioli-Vitti,Luciana; Camargo Abdalla,Fábio; Morelli Silva De Moraes,Regina Lúcia.
The aim of the present study was analyze, by histological and morphometrical studies, mandibular glands of Melipona bicolor queens collected from monogynic and polygynic colonies and compare their level of development. The results showed that the glands of physogastric queens from monogynic colony present a higher level of activity in relation to the queens of polygynic colonies; this is explained by the fact that just a unique queen controls the monogynic colony. In the polygynic colonies, the queens may divide such control to each other.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melipona bicolor; Mandibular gland; Morphology; Polygynic colonies.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000100001

Influence of estradiol analogue on cell growth, morphology and death in esophageal carcinoma cells
Provedor de dados: Biocell
Autores: Mqoco,Thandi; Marais,Sumari; Joubert,Annie.
2-Methoxyestradiol-bis-sulphamate is a bis-sulphamoylated derivative of the naturally occurring 17-beta-estradiol metabolite namely 2-methoxyestradiol. 2-Methoxyestradiol-bis-sulphamate is regarded as a potential anticancer drug with increased antiproliferative activity when compared to 2-methoxyestradiol. The aim of this pilot in vitro study was to determine the influence of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and possible induction of certain types of cell death in the SNO esophageal carcinoma cell line. A dose-dependent study (0.2-1.0µM) was conducted with an exposure time of 24 hours. Data revealed that 2-methoxyestradiol-bis-sulphamate reduced cell numbers statistically significantly to 74% after exposure to 0.4µM of the drug....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; Apoptosis; Autophagy.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000300003

Effect of sugars on the association between cowpea vicilin (7S storage proteins) and fungal cells
Provedor de dados: Biocell
Autores: Rose,T.L.; Gomes,V.M.; Da Cunha,M.; Fernandes,K.V.S.; Xavier-Filho,J.
Vicilins (7S storage proteins) found in various legume seeds have been previously shown to interfere with the germination of spores or conidia of phytopathogenic fungi and inhibit yeast growth and glucose stimulated acidification of the medium by yeast cells. In the present work vicilins from cowpea (Vigna unguiculata) seeds were added to the growth medium of Saccharomyces cerevisiae cells and Fusarium oxysporum conidia. Helix pomatia lectin, wheat germ agglutinin and Ulex europaeus lectin were used to identify differences in the binding of the vicilins to the surface of cells of S. cerevisiae and F. oxysporum treated with this protein. After the growth period, the material in suspension (yeast cells) was centrifuged and the final pellet was also treated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vicilin; Cowpea Seeds; Saccharomyces Cerevisiae; Fusarium Oxysporum.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000200002

Effect of age on the myosin-V immunoreactive myenteric neurons of rats ileum
Provedor de dados: Biocell
Autores: Ferreira Schoffen,João Paulo; Marçal Natali,Maria Raquel.
Alterations in the gastrointestinal neuromuscular function related to age have been demonstrated in human and animal models. This study analyzes the effects of the aging process on the area of the neuronal cell bodies of the myenteric plexus in the antimesenteric and intermediate regions of the ileal circumference of Wistar, 12 month-old in comparison 3 month-old animals. The ileum was removed and whole-mount preparations immunostained by the antibody anti-myosin-V were processed. The morphometric analyses were performed using a computerized image analysis system, with a subsequent distribution of neurons by size in intervals of 100 &amp;#956;m². The cellular body morphometry revealed a significant increase in the size of the myosin-V- immunoreactive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Ileum; Myenteric plexus; Myosin-V; Neuronal plasticity.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100004

Tissue alterations in the Guinea pig lateral prostate following antiandrogen flutamide therapy
Provedor de dados: Biocell
Autores: Cordeiro,Renato Simões; Scarano,Wellerson Rodrigo; Góes,Rejane Maira; Taboga,Sebastião Roberto.
The flutamide antiandrogenic effects on the Guinea pig male prostate morphology in puberal, post-puberal and adult ages were evaluated in the present study. Daily-treated group animals received flutamide subcutaneous injection at a dose of 10 mg/Kg body weight for 10 days. The control group animals received a pharmacological vehicle under the same conditions. The lateral prostate was removed, fixed and processed for light and transmission electron microscopy. The results revealed an increase of the acinus diameter in the treated puberal animals and straitness in the stromal compartment around the acini. The epithelial cells exhibited cubic phenotype. In the post-puberal and adult animals, a decrease of the acinus diameter was observed, as well as an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lateral Prostate; Flutamide; Antiandrogen; Secretory Cells.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000100003

Morphological study of the spermatogenesis in the teleost Piaractus mesopotamicus
Provedor de dados: Biocell
Autores: da Cruz-Landim,Carminda; Camargo Abdalla,Fábio; da Cruz-Höfling,Maria Alice.
The spermatogenesis of Piaractus mesopotamicus was investigated under light and transmission electron microscopy. The specimens were captured from their natural environment (Rio Miranda and Rio Aquidauana, Pantanal Matogrossense, Brazil) during April and September. The results were compared with the spermatogenic data of specimens under captivity condition. In both conditions, P. mesopotamicus presented the typical spermatogenesis pattern of the teleost fishes, showing no significative differences. The spermatozoon was classified as type I, which has a globular head without acrosome, a short middle piece and a long tail constituted only by the flagellum. This type of spermatozoon is considered the basic type in fishes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Piaractus mesopotamicus; Spermatogenesis; Spermatogonia; Spermiogenesis; Teleostei.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300003

CAS role in the brain apoptosis of Bufo arenarum induced by Cypermethrin
Provedor de dados: Biocell
Autores: Izaguirre,M. F.; Vergara,M. N.; Casco,V. H.
CAS might have a key role in the apoptosis induced by toxins, acting as anti-apoptotic factor, stimulating the cellular proliferation and the cell contact stabilization. To start to elucidate their role in the brain apoptosis of Bufo arenarum induced by cypermethrin (CY), the expression patterns of CAS and several cell adhesion molecules (CAMs) were established. Bufo arenarum tadpoles of the control and acute bioassay survival at different doses (39, 156, 625 and 2,500 µg CY/L) and times (24, 48, 72 and 96 h) of CY treatment were fixed in Carnoy, embedded in paraffin and sectioned. CAS and CAMs expression was determined by immunofluorescence and immunohistochemistry, respectively. When the bioassay starts, CAS increases suggesting a proliferative or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Brain; Cypermethrin; CAS; Cams.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200007

Micropropagation of Ilex dumosa (Aquifoliaceae) from nodal segments in a tissue culture system
Provedor de dados: Biocell
Autores: Luna,C.; Sansberro,P.; Mroginski,L.; Tarragó,J.
Micropropagation of Ilex dumosa var. dumosa R. ("yerba señorita") from nodal segments containing one axillary bud was investigated. Shoot regeneration from explants of six-year-old plants was readily achieved in ¹/4 strength Murashige and Skoog medium (¹/4 MS) plus 30 gr·L-1 sucrose and supplemented with 4.4 mM BA. Further multiplication and elongation of the regenerated shoots were obtained by subculture in a fresh medium of similar composition with 1.5 gr·L-1 sucrose. Rooting induction from shoots were achieved in two steps: 1) 7 days in ¹/4 MS (30 gr·L-1 sucrose, 0.25 % Phytagel®) with 7.3 mM IBA and 2) 21 days in the same medium without IBA and 20 mM of cadaverine added. Regenerated plants were successfully transferred to soil. This micropropagation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Nodal Segment; Cytokinins; Ilex Dumosa.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000200006

Orbicules diversity in Oxalis species from the province of Buenos Aires (Argentina)
Provedor de dados: Biocell
Autores: Rosenfeldt,Sonia; Galati,Beatriz Gloria.
Eleven Oxalis L. species from the province of Buenos Aires (Argentina) were investigated with scanning and transmission electron microscopes. We identified four different types and two subtypes of orbicules. We conclude that the close morphological similarity between these species is also reflected in their orbicules, and we suggest that the orbicules morphology may be a useful character in systematic studies.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryology; Orbicules; Oxalis; Taxonomy; Pollen.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000100005

Distribution of pectins in the pollen apertures of Oenothera hookeri.velans ster/+ster:
Provedor de dados: Biocell
Autores: Noher de Halac,I.; Cismondi,I.A.; Rodriguez-Garcia,M.I.; Famá,G.
Cell wall pectins are some of the most complex biopolymers known, and yet their functions remain largely mysterious. The aim of this paper was to deepen the study of the spatial pattern of pectin distribution in the aperture of Oenothera hookeri.velans ster/+ster fertile pollen. We used "in situ" immunocytochemical techniques at electron microscopy, involving monoclonal antibodies JIM5 and JIM7 directed against pectin epitopes in fertile pollen grains of Oenothera hookeri.velans ster/+ster. The same region was also analyzed by classical cytochemistry for polysaccharide detection. Immunogold labelling at the JIM7 epitope showed only in mature pollen labelling mainly located at the intine endo-aperture region. Cytoplasmic structures near the plasma membrane...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pectins; Monoclonal antibodies; JIM5; JIM7; Immunogold; Pollen; Sporoderm; Oenothera; Cytochemistry.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000100002

Models for the recent evolution of protocadherin gene clusters
Provedor de dados: Biocell
Autores: Morgan,Marcos.
The clustered protocadherins (Pcdhs) are single-pass transmembrane proteins that constitute a subfamily within the cadherin superfamily. In mammals, they are arranged in three consecutive clusters named alpha, beta, and gamma. These proteins are expressed in the nervous system and are targeted to mature synapses. Interestingly, different neurons express different subsets of isoforms; however, little is known about the functions and expression of the clustered Pcdhs. Previous phylogenetic analyses that compared rodent and human clusters postulated the recent occurrence of gene duplication events. Using standard phylogenetic methods, I confirmed the prior observations, and I show that duplications are likely to occur through unequal crossing-over events...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protocadherin; Gene birth; Gene conversion; Cluster evolution.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000100002

Cloning and analysis of IFRG (interferon responsive gene) in rabbit oocytes and preimplantation embryos
Provedor de dados: Biocell
Autores: Qi,Bing; Zheng,Xinming; Li,Wenyong; Wei,Qingxin; Chen,Qingxuan.
Although there is more evidence that shows that IFNs (interferons) plays a very important role in the early development of the embryo, the mechanism of IFNs is still unclear. Our study showed that IFRG is expressed from oocytesthrough to the preimplantation embryo in rabbits. This finding provides some clues for better understanding the role of IFNs in the development of the embryo. The full length of rabbit IFRG cDNA (Accession No. AJ584672), with a 2794bp encoding 131 amino acid sequence, was cloned IFRG expression can be detected in 8 different tissues: ovary, heart, lung, liver, kidney, spleen, cerebra, and the 18-day whole-body embryo. Whole-mount in situ hybridization showed that IFRG was highly expressed in the inner-cell mass of rabbit blastula....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Preimplantation embryo development; Interferon a responsive gene; Whole mount in situ hybridization.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000200001

Storage lipids and proteins of Euterpe edulis seeds
Provedor de dados: Biocell
Autores: Panza,Vïctor; Pighin,Dario; Láinez,Verónica; Pollero,Ricardo J.; Maldonado,Sara.
Comparative studies on fatty acid and protein composition of the endosperm and embryo of palmito (Euterpe edulis Martius) were conducted using gas-liquid chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. On a dry weight basis, the embryo contained extremely lower amounts of lipids and proteins than did the endosperm, which was associated with the scarce lipid and protein bodies previously reported in axis and cotyledon. The fatty acid composition also exhibited differences between both tissues: (I) the fatty acid diversity was greater in embryo than in endosperm; (II) embryo and endosperm contained predominantly linoleic, palmitic, oleic and stearic acids even though the relative values were different for each tissue. As...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Palm seeds; Arecaceae; Fatty acids; Recalcitrant behavior; Storage susceptibility.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200004

Salicylic acid permeation: A comparative study with different vehicles and membranes
Provedor de dados: Biocell
Autores: Olivella,Mónica S.; Debattista,Nora B.; Pappano,Nora B.
Considering the skin´s function, different dermal pharmaceutical forms can be developed according to the type of therapeutic activity, active principle and excipients involved in the formulation, such as “transdermal preparations”. In the present study, the permeation parameters of the non-steroidal antiinflammatory drug, salicylic acid (SA) through synthetic membrane, polyvinyliden difluoride, and a biological membrane, egg shell membrane, with different vehicles, propylene glycol, isopropyl alcohol and carbopol gel, were determined. The reported physicochemical parameters of SA from CG were significantly higher than those obtained using PG and IP. This is attributed to the lipophilic nature of the vehicle that facilitates the release and penetration of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Membranes; Permeation; Drug release; Salicylic acid.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200008

Influence of genotype and explant source on indirect organogenesis by in vitro culture of leaves of Melia azedarach L.
Provedor de dados: Biocell
Autores: VILA,S.K.; REY,H.Y.; MROGINSKI,L.A.
In vitro regeneration of shoots from leaf explants of the Paradise tree (Melia azedarach L.) was studied. Three different portions (proximal portion, distal portion and rachis of the leaflets) of three developmental stages (folded, young still expanding and completely expanded) of leaves of 10 - 15 years old plants of seven genotypes were cultured on Murashige and Skoog (1962) medium (MS) supplemented with 1mg.l-1 benzylaminopurine (BAP) + 0.1mg.l-1 kinetin (KIN) + 3 mg.l-1 adenine sulphate (ADS). The rachis of the leaflets of the completely expanded leaves was found to be the most responsive tissue, in most of the genotypes employed. Shoot regeneration occurred in leaf explants of all the genotypes tested. The best genotype for shoot regeneration was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotype; Leaf explants; Organogenesis; Paradise tree; Plant regeneration.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000100005

Karyological and electrophoretic differences between Pomacea flagellata and P. patula catemacensis: Caenogastropoda: Ampullariidae
Provedor de dados: Biocell
Autores: Diupotex-Chong,María Esther; Cazzaniga,Néstor J; Uribe-Alcocer,Manuel.
The widespread Mexican apple snail Pomacea flagellata (Say 1827) and the strictly endemic "tegogolo" P. patula catemacensis (Baker 1922) (restricted to Lake Catemaco), are the only known American Ampullariidae that have haploid complements n=13. Pomacea patula catemacensis has suffered a critical reduction in abundance due to immoderate fishing for human consumption. Chromosome slides were obtained from colchicine-injected Pomacea snails collected from nine locations along the coastal zone of the Gulf of Mexico, including Lake Catemaco, for use in principal component analysis (PCA). Total proteins in foot homogenates were analyzed through isoelectric focusing (IEF) and native-PAGE electrophoresis on polyacrylamide gels. The chromosome number 2n=26 was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apple snails; Endangered species; Isoelectric focusing; Karyotype; Mexico; Native-PAGE electrophoresis.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300002

Effects of the ascorbic acid supplementation on NADH-diaphorase myenteric neurons in the duodenum of diabetic rats
Provedor de dados: Biocell
Autores: Pereira,Marli Aparecida Dos Santos; Bagatin,Maria Claúdia; Zanoni,Jacqueline Nelisis.
We assessed the ascorbic acid (AA) supplementation on the myenteric neurons in the duodenum of rats. Fifteen rats with 90 days of age were divided into three groups: control (C), diabetics (D) and ascorbic acid treated diabetics (DA). After 120 days of daily treatment with AA, the duodenum was submitted to the NADH-diaphorase (NADH-d) histochemical technique, which allowed us to evaluate the neuronal density in an area of 8.96 mm² for each duodenum, and also to measure the cellular profile area of 500 neurons per group. The supplementation promoted an increase on AA levels. The neuronal density (p &lt;0.05) was higher in the group DA when compared to group D. There were no significant differences in the neuronal areas, when we compared groups C (204 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; Ascorbic acid; Myenteric neurons.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200005

A standard weight equation to assess the body condition of pejerrey Odontesthes bonariensis
Provedor de dados: Biocell
Autores: Colautti,Darío C.; Remes Lenicov,Mauricio; Berasain,Gustavo E.
We developed a standard weight equation Ws to aid in the analysis of pejerrey Odonthestes bonariensis body condition over time and across populations using the relative weight index Wr. Weight - length data were compiled from 73 populations of pejerrey (N=16.022) from the Argentine pampas region. We used the regression-line-percentile technique, which provides a 75th-percentile standard by length intervals of 10 mm, to develop the Ws equation. The proposed equation is log10 Ws=-5,267+3,163 log10 Lst; Ws is weight in grams and Lst is standard length in millimeters. This equation is proposed for use with pejerrey between 120 and 520 mm of Lst. Values for Wr calculated with the Ws equation did not consistently increase or decrease as function of fish length,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Standard weight; Pejerrey; Odontesthes bonariensis; Condition.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100017

AlejandraLaura Estebenet: 1960-2005
Provedor de dados: Biocell
Tipo: Info:eu-repo/semantics/report
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200009

Protein detection in spermatids and spermatozoa of the butterfly Euptoieta hegesia (Lepidoptera)
Provedor de dados: Biocell
Autores: Mancini,Karina; Dolder,Heidi.
This study was undertaken to detect protein components in both sperm types of the butterfly Euptoieta hegesia. These spermatozoa possess complex extracellular structures for which the composition and functional significance are still unclear. In the apyrene sperm head, the proteic cap presented an external ring and an internal dense content; basic proteins were detected only in external portions. In the tail, the paracrystalline core of mitochondrial derivatives and the axoneme are rich in proteins. The extratesticular spermatozoa are covered by a proteic coat, which presented two distinct layers. In eupyrene spermatozoa, acrosome and nucleus were negatively stained, probably because of their high compaction. In the tail, there is no paracrystalline core...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ultrastructure; Protein; Apyrene; Eupyrene.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000300006

A six-rhabdomere, open rhabdom arrangement in the eye of the chrysanthemum beetle Phytoecia rufiventris: some ecophysiological predictions based on eye anatomy
Provedor de dados: Biocell
Autores: Meyer-Rochow,Victor Benno; Mishra,Monalisa.
We are describing a rhabdom organization of the eye of the chrysanthemum beetle Phytoecia rufiventris that to date has not been described from any other insect. In cerambycid beetles free rhabdomeres, forming a circular, open rhabdom, surround a central rhabdom made up of the rhabdomeres of one or two cells. In Phytoecia rufiventris the central rhabdomeres are missing throughout the eye and the microvilli of the outer 6 rhabdomeres are regularly oriented in three directions. Following the classification of rhabdom types suggested by Wachmann (1979), we suggest to name the rhabdom arrangement seen in the retina of Phytoecia rufiventris "Grundmuster 3". This pattern ought to facilitate polarization sensitivity and movement perception, features that agree...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Compound eye; Retina; Vision; Polarization sensitivity.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200006

Role of mast cells in gastrointestinal mucosal defense
Provedor de dados: Biocell
Autores: Penissi,Alicia; Rudolph,María; Piezzi,Ramón.
The purpose of this review, based on studies from our laboratory as well as from others, is to summarize salient features of mast cell immunobiology and to describe their associations with gastrointestinal mucosal defense. Gastrointestinal mast cells are involved in many pathologic effects, such as food hypersensitivity. On the other hand, they also play a protective role in defense against parasitic and microbial infections. Thus, they have both positive and negative effects, but presently the mechanisms that control the balance of these various effects are poorly known. It has been suggested that stabilization of mast cells may be a key mechanism to protect the gastrointestinal tract from injury. Few molecules are known to possess both mast cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mast Cells; Mucosal Defense; Gastrointestinal; Dehydroleucodine.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000200001

Secondary zoospores in the algal endoparasite Maullinia ectocarpii (Plasmodiophoromycota)
Provedor de dados: Biocell
Autores: Parodi,Elisa R.; Cáceres,Eduardo J.; Westermeier,Renato; Müller,Dieter G.
The present paper deals with the ultrastructure of zoospores produced by the plasmodiophorid Maullinia ectocarpii, living in the marine algal host Ectocarpus siliculosus. The zoospores described here are very similar to secondary zoospores of Polymyxa graminis and Phagomyxa sp.(the latter an algal endoparasite, also). Our results indicate that M. ectocarpii produces two types of plasmodia, and suggest that is a species with a complete life cycle, as it is known for all the Plasmodiophormycota that have been studied. Sporogenic and sporangial plasmodia produce, respectively, primary zoospores with parallel flagella within thick walled resting sporangia, and secondary zoospores with opposite flagella within thin walled sporangia.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intracellular parasites; Flagellar apparatus; Plasmodiophoromycota; Brown algae; Ectocarpales.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000100006

Single-channel response of hamster oocytes to ertilization with homologous spermatozoa
Provedor de dados: Biocell
Autores: Ituarte,Leonor M.E.; Viera,Teresa B.; Saldeña,Teobaldo A.; De Rosas,Juan C.; Fóscolo,Mabel; Ibáñez,Jorge E.; Saraví,Fernando D.
Electrophysiological events occur early after fertilization, along with changes in intracellular Ca2+ concentration . Passive electrical parameters were determined in golden hamster oocytes by whole cell patch-clamp method. In separate experiments the effect of 4-aminopyridine on resting oocytes was tested. The single-channel patch clamp configuration was employed to assess the electrical response to fertilization with homologous sperm. Structure of oocytes submitted to patch clamp was evaluated with scanning electron microscopy and found to be preserved. Oocyte diameter was 70.2 ±  2.2 µm; their resting parameters were: membrane potential 23.8 ±  0.8 mV; total membrane specific resistance 519.1 ±  94.6 &amp;#937;.cm², and specific capacity 0.99 ±  0.03...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 4-Aminopyridine; Fertilization; Hamster oocyte; Patch-clamp; Membrane current.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100007

Goblet cell number in the ileum of rats denervated during suckling and weaning
Provedor de dados: Biocell
Autores: Hernandes,Luzmarina; Marques da Silva Pereira,Lucieni Cristina; Parisi Alvares,Eliana.
The enteric nervous system plays a role on the stimulation of secretory cells of intestinal epithelia. We have demonstrated that ablation of ENS stimulates epithelial cell proliferation. As goblet cells are important constituents of the epithelial sheet, it is mandatory to investigate separately this cell type. The myenteric plexus of the ileum of rats in postnatal development was partially removed by the serosal application of benzalkonium chloride (BAC). Three groups of animals were used: those where BAC application was at 13 days and sacrifice was at 15 (13/28-day-old) or 23 days after treatment (13/36-day-old), and those where BAC was applied at 21 days and rats were killed 15 days after treatment (21/36-day-old) . The number of goblet cells in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Benzalkonium chloride; Development; Goblet cells; Myenteric plexus; Small intestine.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300005

Systematic revision of the South American silversides (Teleostei, Atheriniformes)
Provedor de dados: Biocell
Autores: Brian,S.; Dyer,H.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherinopsinae; Odontesthes; Phylogeny; Taxonomy; Morphology.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100010

Fine-structural details of the photoreceptor membranes in the ocellus of the scale-insect parasite Centrodora sp. (Hymenoptera; Aphenelidae):: A case of gene transfer between host and parasite?
Provedor de dados: Biocell
Autores: Yang,Mouyu; Meyer-Rochow,V.Benno.
Only one insect (the scale insect Eriococcus sp.) is known, in which photoreceptive lamellae appear to have replaced the usual arthropod rhabdom microvilli. We are now reporting the presence of photoreceptive membranes, which also appear to resemble lamellae rather than microvilli, but they are in the ocellus of the tiny wasp Centrodora sp., which parasitizes scale insect eggs. The apparently optically homogenous lens of the Centrodora ocellus measures approximately 10 µm in diameter and, thus, operates at the limits of diffraction. We calculated that the lens is capable of focusing a parallel bundle of rays on the retina of the ocellus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insect retina; Eye; Ocellus; Parasitoid; Visual membrane.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000200004

Natural spawning and intensive culture of pejerrey Odontesthes bonariensis juveniles
Provedor de dados: Biocell
Autores: Miranda,L.A.; Berasain,G.E.; Velasco,C.A.M.; Shirojo,Y.; Somoza,G.M.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquaculture; Pejerrey; Reproduction; Seed production.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100020

Creatine supplementation in trained rats causes changes in myenteric neurons and intestinal wall morphometry
Provedor de dados: Biocell
Autores: Franzói De Moraes,Solange Marta; Brogio,Thais Andréia; Nelisis Zanoni,Jacqueline; Vicente Umada Zapater,Mariana Cristina; Barnabé Peres,Sidney; Hernandes,Luzmarina.
Creatine is widely used by athletes as an ergogenic resource. The aim of this study was to evaluate the influence of creatine supplementation on the duodenum of rats submitted to physical training. The number and myenteric neuronal cell bodies as well mucosal and muscular tunic morphometry were evaluated. Control animals received a standard chow for 8 weeks, and the treated ones received the standard chow for 4 weeks and were later fed with the same chow but added with 2% creatine. Animals were divided in groups: sedentary, sedentary supplemented with creatine, trained and trained supplemented with creatine. The training consisted in treadmill running for 8 weeks. Duodenal samples were either processed for whole mount preparations or for paraffin embedding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nutritional supplementation; Duodenum; Exercise.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000200004

WT-1 mRNA expression is modulated by nitric oxide availability and Hsp70 interaction after neonatal unilateral ureteral obstruction
Provedor de dados: Biocell
Autores: Mazzei,Luciana; García,Isabel Mercedes; Cacciamani,Valeria; Benardón,María Eugenia; Manucha,Walter.
Wilms tumor gene 1 (wt-1), a key regulator of mesenchymal-epithelial transformation, is downregulated during congenital obstructive nephropathy, leading to apoptosis. There is a functional interaction between WT-1 and inducible nitric oxide synthase (iNOS). In this regard, we reported that after neonatal unilateral ureteral obstruction, rosuvastatin prevents apoptosis through an increase in nitric oxide bioavailability, which in turn is linked to higher Hsp70 expression. Hence, the goal of this study was to determine whether a nitric oxide/Hsp70 interaction is involved in changes in WT-1 mRNA expression after ureteral obstruction. Neonatal rats submitted to experimental ureteral obstruction were treated with either vehicle or rosuvastatin for 14 days....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obstructive nephropathy; Apoptosis; Statins; Kidney development.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000300004

Protective effect of aqueous suspension of dried latex of Calotropis procera against oxidative stress and renal damage in diabetic rats
Provedor de dados: Biocell
Autores: Kumar,Vijay L.; Padhy,Biswa M.
Calotropis species have been used in the traditional medicinal system for the treatment of diseases of the liver and abdomen. In view of the antioxidant and anti-hyperglycemic properties of an aqueous suspension obtained from the dried latex of Calotropis procera, the present study was carried out to evaluate its efficacy in affording protection against alloxan induced changes in rat kidney. A single intraperitoneal injection of alloxan (150 mg/kg) in rats produced hyperglycemia within 3 days and altered kidney functions over a period of 90 days. Daily oral administration of the aqueous suspension (100 and 400 mg/kg) in diabetic rats produced anti-hyperglycemic effect that was comparable to that of glibenclamide (10 mg/kg). Unlike glibenclamide, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Kidney; Anti-oxidant; Anti-hyperglycemic.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000300001

Evaluation of a multiplex PCR method to detect enteroaggregative Escherichia coli
Provedor de dados: Biocell
Autores: Rüttler,M.E.; Yanzón,C.S.; Cuitiño,M.J.; Renna,N.F.; Pizarro,M.A.; Ortiz,A.M.
Enteroaggregative Escherichia coli (EAEC) has been implicated in sporadic diarrhea in children and adults and has been identified as the cause of several outbreaks worldwide. The HEp-2 test remains the gold standard for identification of this pathotype. A 60-65 MDa plasmid encodes the aggregative adherence fimbriae (AAF/I and AAF/II), a transcriptional activator ( aggR gene), the enteroaggregative heat-stable enterotoxin EAST1 ( astA gene) and a cytotoxin (Pet). The standard assay for EAEC is performed only in research laboratories, because it is expensive, labor intensive and time-consuming. The Polymerase Chain Reaction (PCR) offers the possibility of rapid diagnosis. In the current study, a multiplex PCR assay which checks aggR and astA genes was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Escherichia coli; Cell culture; PCR (Polymerase Chain Reaction).
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200006

Antiproliferative and genotoxic effects of Mikania glomerata (Asteraceae)
Provedor de dados: Biocell
Autores: Dalla Nora,Gracieli; Pastori,Tamara; Dail Laughinghouse IV,Haywood; Do Canto-Dorow,Thais Scotti; Bosio Tedesco,Solange.
Mikania glomerata is a plant used in Brazilian traditional medicine, known as &amp;#8216;guaco'. It possesses anti-inflammatory properties and the aqueous extracts of its leaves are indicated for the treatment of diseases of the respiratory tract. This study aimed at evaluating the antiproliferative and genotoxic effect of Mikania glomerata leaf infusions on the cell cycle of onion. The material used was collected in the native environment from Rio Grande do Sul State, Brazil. Aqueous extracts through infusions were prepared in two concentrations: 4g/L (usual concentration) and 16g/L (4x more concentrated) of each of the populations. Two groups of four onion bulbs for each plant population were used plus a control group. The rootlets were fixed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plant; Allium cepa test; Chromosomal aberration.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000300001

Agmatine inhibits hypoxia-induced TNF-alpha release from cultured retinal ganglion cells
Provedor de dados: Biocell
Autores: Hong,Samin; Park,Kyoungsoo; Kim,Chan Yun; Seong,Gong Je.
The effect of hypoxia on the release of tumor necrosis factor-alpha (TNF-alpha) in transformed rat retinal ganglion cells (RGCs) and the effect of agmatine on the hypoxia-induced production of TNF-alpha in RGCs were evaluated. RGCs were cultured under hypoxic conditions with 5% oxygen, with or without 100&amp;#956;M agmatine. The expression levels of TNF-alpha and its receptor-1 (TNF-R1) were investigated by Western blot analysis. After 6 hours of hypoxia, we noted an increase in TNF-alpha production in RGCs. Agmatine significantly reduced TNF-alpha level after 12 hours of hypoxic treatment. The expression of TNF-R1 was not affected by the hypoxia or agmatine treatment. Our results show that agmatine inhibits the TNF-alpha production of RGCs in hypoxic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Retinal ganglion cell; Tumor necrosis factor-alpha; Hypoxia; Agmatine.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200006

Pollen morphology of Oxalis species from Buenos Aires province (Argentina)
Provedor de dados: Biocell
Autores: Rosenfeldt,Sonia; Galati,Beatriz G.
Pollen morphology and the degree of pollen variability within nine species and two varieties of Oxalis species from Buenos Aires Province (Argentina) were studied using light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Oxalis pollen grain is generally 3-colpate and the shape is prolate spheroidal, oblate spheroidal or spheroidal. The exine is microreticulate. The brochi are circular to polygonal; brochi sizes decrease near the colpi. The colpus membrane observed in unacetolysed material, is crustate or granular. The colpus length varies within a species; the largest grains are produced in stamens with long filaments and the smallest ones in stamens with short filaments. Four pollen types are distinguished...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pollen; Oxalis; Morphology; Structure.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100002

Intrahost distribution and trasmission of a new species of cyclopoid copepod endosymbiotic to a freshwater snail, Pomacea canaliculata (Caenogastropoda, Ampullariidae) from Argentina
Provedor de dados: Biocell
Autores: Gamarra-Luques,C. D.; Vega,I. A.; Koch,E.; Castro-Vazquez,A.
A new species of cyclopoid copepod, Ozmana huarpium, is described as a symbiont to Pomacea canaliculata (Lamarck 1822) (Caenogastropoda, Ampullariidae). Rather large numbers (about one hundred copepods per snail) were found, although there was no evidence of harm to the host. To our knowledge, O. haemophila (symbiont to P. maculata), and the currently described species, O. huarpium, are the only copepod species ever recorded as endosymbionts to freshwater invertebrates. While O. haemophila is restricted to the haemocoel of its host, O. huarpium predominate in the penis sheath, the ctenidium and the mantle cavity, figuring in these pallial organs 63-65 % of total mature forms. The sex ratio of the symbiont is skewed to the female side in these organs,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ozmanidae; Ozmana haemophila; Ozmana huarpium. n. sp.; Pallial organs; Haemocoel.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000200005

Autophagy, apoptosis and organelle features during cell exposure to cadmiumc
Provedor de dados: Biocell
Autores: dos Santos Vergilio,Cristiane; Tenório de Melo,Edésio José.
Cadmium (Cd) induces several effects in different tissues, but our knowledge of the toxic effects on organelles is insufficient. To observe the progression of Cd effects on organelle structure and function, HuH-7 cells (human hepatic carcinoma cell line) were exposed to CdCl2 in increasing concentrations (1 &amp;#956;M - 20 &amp;#956;M) and exposure times (2 h - 24 h). During Cd treatment, the cells exhibited a progressive decrease in viability that was both time- and dose-dependent. Cd treated cells displayed progressive morphological changes that included cytoplasm retraction and nuclear condensation preceding a total loss of cell adhesion. Treatment with 10 &amp;#956;M for 12 h led to irreversible damages. Before these drastic and irreparable damages,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cd; Cell death; Hepatocyte; HuH-7 cells; Mitochondria.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000200005

Morphology and structure of the pollen cone and pollen grain of the Araucaria species from Argentina
Provedor de dados: Biocell
Autores: Fueyo,Georgina M. del; Caccavari,Marta A.; Dome,Elizabeth A.
The pollen cone and the pollen grain of the two Argentinean species of Araucaria are described with LM, SEM and TEM. Primordia of pollen cones are formed in April and May and reach maturity by mid-October in A. angustifolia (Bert.) O. Kuntze and by mid-November in A. araucana. (Mol.) K. Koch. Characters of the mature pollen cones and microsporophylls between both taxa are clearly differentiated. Pollen grains are spheroidal-subspheroidal, inaperturate, and asaccate with granulate exine and a subequatorial annular area that corresponds to the sexine thickness. Sculpturing consists of irregularly dispersed granules that are sometimes fused to each other (A. angustifolia) or forming microrugulae (A. araucana). Microgranules and microspinules are also present....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Araucariaceae; Araucaria; Pollen cones; Pollen ultrastructure.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000100006

A cryopreservation protocol for immature zygotic embryos of species of Ilex (Aquifoliaceae)
Provedor de dados: Biocell
Autores: Mroginski,Luis A.; Sansberro,Pedro A.; Scocchi,Adriana M.; Luna,Claudia; Rey,Hebe Y.
Tropical Ilex species have recalcitrant seeds. This work describes experiments demonstrating the feasibility of long-term conservation of Ilex brasiliensis, I. brevicuspis, I. dumosa, I. intergerrima, I. paraguariensis, I. pseudoboxus, I. taubertiana, and I. theezans through cryopreservation of zygotic rudimentary embryos at the heart developmental stage. The embryos were aseptically removed from the seeds and precultured (7 days) in the dark, at 27± 2ºC on solidified (0.8% agar) 1/4MS medium, [consisting of quarterstrength salts and vitamins of Murashige and Skoog (1962) medium] with 3% sucrose and 0.1 mg/l Zeatin. The embryos were then encapsulated in 3% calcium alginate beads and pretreated at 24 h intervals in liquid medium supplemented with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Encapsulation-dehydration; Germplasm preservation; Liquid nitrogen; Llex.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000100004

Reproductive performance of the Mesa silverside (Chirostoma jordani Woolman, 1894) under natural and controlled photoperiods
Provedor de dados: Biocell
Autores: Arredondo-Figueroa,José Luis; Núñez-García,Laura Georgina; Heredia-Guzmán,Paloma Adriana; Ponce-Palafox,Jesús T.
Chirostoma jordani is a native annual species inhabiting lacustrine waters of the Central Mexico Plateau. It is widely distributed and is currently facing high environmental pressures. Five experiments were performed to study the reproductive performance of this species. Four of the experiments were conducted in 270-L indoor recirculation tanks. Two males and one female at the first stage of reproduction were included in each test. A photoperiod of 14 light hours and 10 dark hours was used. In a fifth experiment, 10 females and 15 males were kept in an outdoor 3,000-L recirculation tank under natural photoperiod. The number of spawns, fertilised eggs and 30-day-old juveniles were counted and the survival rate was calculated. The results indicated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breeding; Atherinopsidae; Eggs; Juveniles; Survival rate.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300002

An integrative view to structure, function, ontogeny and phylogenetical significance of the male genital system in Pomacea canaliculata (Caenogastropoda, Ampullariidae)
Provedor de dados: Biocell
Autores: Gamarra-Luques,C.; Winik,B.C.; Vega,I.A.; Albrecht,E.A.; Catalan,N.M.; Castro-Vazquez,A.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spermatogenesis; Steroids; Peptides; Imposex; Xenobiotics.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200012

A unique mechanism of nuclear division in Giardia lamblia involves components of the ventral disk and the nuclear envelope
Provedor de dados: Biocell
Autores: Solari,Alberto J.; Rahn,Monica I.; Saura,Alicia; Lujan,Hugo D.
The fine structure of the binucleate, parasitic protist Giardia lamblia during interphase and divisional stages was studied by serial thin sectioning and three-dimensional reconstructions. The earlier sign of nuclear division is the development of a few peripheral areas of densely packed chromatin directly attached to the inner nuclear envelope. An intracytoplasmic sheet of ventral disk components grows from the cell periphery towards one of the nuclei, apparently constricting this nucleus, which becomes located at a ventral bulge. After the basal bodies become duplicated, a full nuclear division occurs in trophozoites, giving two pairs of parent-daughter nuclei. This full division occurs in a dorsal-ventral direction, with the resulting nuclear pairs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Giardia; Primitive mitosis; Fine structure of mitosis; Protist mitosis; Cell differentiation.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300004

Modulation of IL-10/IL-10R expression by mafosfamide, a derivative of 4-hydroxycyclophosphamide, in a rat B-cell lymphoma
Provedor de dados: Biocell
Autores: Rico,María J.; Matar,Pablo; Scharovsky,O. Graciela.
We have already shown that IL-10 plays an important role in immunosuppression and metastatic dissemination in the rat B-cell lymphoma L-TACB model. It was suggested that the up-regulation of IL-10 production and IL-10 receptor (IL-10R) expression would be part of the transition from primary tumor to metastatic phenotype and that IL-10, besides its immunosuppressive activity, may act as a growth factor for metastatic L-TACB cells. The treatment of L-TACB-bearing rats with a single low-dose cyclophosphamide decreased IL-10 production, reverted immunosuppression and induced the immunologic rejection of tumor metastasis without any effect on primary tumor growth. Our current aim was to investigate the effects of cyclophosphamide on the expression of IL-10 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metastasis; Cyclophosphamide; Cell proliferation.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000200006

Ultrastructural effects of two phthalocyanines in CHO-K1 and HeLa cells after laser irradiation
Provedor de dados: Biocell
Autores: de CastroPazos,Marcelo; Pacheco-Soares,Cristina; Soares da Silva,Newton; DaMatta,Renato Augusto; Pacheco,Marcos Tadeu T.
The effects of Photodynamic Therapy using 2nd generation photosensitizers have been widely investigated aiming clinical application treatment of solid neoplasms. In this work, ultrastructure changes caused by the action of two 2nd generation photosensitizers and laser irradiation on CHO-K1 and HeLa (neoplastic) cells were analyzed by transmission electron microscopy. Aluminum phthalocyanine chloride, aluminum phthalocyanine tetrasulfonate chloride and radiation from a semiconductor laser at a fluency of 0.5 J/cm² (Power=26mW; l=670nm) were used. The results showed induction of apoptosis. Such alterations where observed in HeLa but not in CHO-K1 cells after Aluminum phthalocyanine tetrasulfonate chloride (AlPcS4) photodynamic treatment. The Aluminum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photodynamic therapy; Phthalocyanines; Laser; Cell death; Ultrastructure.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300001

Stimulation of jasmonic acid production in Zea Mays L. infected by the maize rough dwarf virus - Río Cuarto: Reversion of symptoms by salicylic acid
Provedor de dados: Biocell
Autores: Vigliocco,A.; Bonamico,B.; Alemano,S.; Miersch,O.; Abdala,G.
In the present paper we study the possible biological relevance of endogenous jasmonic acid (JA) and exogenous salicylic acid (SA) in a plant-microbial system maize-virus. The virus disease "Mal de Río Cuarto" is caused by the maize rough dwarf virus - Río Cuarto. The characteristic symptoms are the appearance of galls or "enations" in leaves, shortening of the stem internodes, poor radical system and general stunting. Changes in JA and protein pattern in maize control and infected plants of a virus-tolerant cultivar were investi-gated. Healthy and infected-leaf discs were collected for JA measurement at different post-infection times (20, 40, 60 and 68 days). JA was also measured in roots on day 60 after infection. For SDS-PAGE protein analysis, leaf...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Maize; Virus disease; Jasmonic and salicylic acids.
Ano: 2002
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452002000300007

The actin filament network associated to Sertoli cell ectoplasmic specializations
Provedor de dados: Biocell
Autores: Cavicchia,Juan Carlos; Fóscolo,Mabel; Ibañez,Jorge; Lillig,Christopher; Capani,Francisco.
Junctional devices in Sertoli cells conform the blood-testis barrier and play a key role in maturation and differentiation of germ cells. The spacial distribution of ectoplasmic specializations of Sertoli cells was studied by &amp;beta;-actin immunolabelling, using laser confocal and transmission electron microscopy. For confocal microscopy, &amp;beta;-actin immunolabelling of ectoplasmic specializations was studied over the background of either prosaposin or glutaredoxin immunolabelling of the Sertoli cytoplasm. Labelling was found near the basal lamina, surrounding early spermatocytes (presumably in leptotene-zygotene) or at one of two levels in the seminiferous epithelium: (1) around deep infoldings of the Sertoli cell cytoplasm, in tubular stages...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: &amp;beta; -actin Ectoplasmic specializations Sertoli cell Seminiferous epithelium.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000300003

Simvastatin acts as an inhibitor of interferon gamma-induced cycloxygenase-2 expression in human THP-1 cells, but not in murine RAW264.7 cells
Provedor de dados: Biocell
Autores: Lee,Chang Seok; Shin,Yong Jae; Won,Cheolhee; Lee,Yun-Song; Park,Chung-Gyu; Ye,Sang-Kyu; Chung,Myung-Hee.
Cyclooxygenase-2 (COX-2) is a key inflammatory response molecule, and associated with many immune functions of monocytes/macrophages. Particularly, interferon gamma (IFNgamma)-induced COX-2 expression appears in inflammatory conditions such as viral infection and autoimmune diseases. Recently, statins have been reported to show variable effects on COX-2 expression, and on their cell and species type dependences. Based on the above description, we compared the effect of simvastatin on IFNgamma-induced COX-2 expression in human monocytes versus murine macrophages. In a result, we found that simvastatin suppresses IFNgamma-induced COX-2 expression in human THP-1 monocytes, but rather, potentiates IFNgamma-induced COX-2 expression in murine RAW264.7...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monocyte; Macrophage; INFgamma; STAT1/3; SOCS1/3.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200005

Micrometric measurement of the density of stained odontoblast processes
Provedor de dados: Biocell
Autores: Kohli,Alicia; Pezzotto,Stella M.; Garcia,Graciela; Poletto,Leonor C.
The embryological, structural and functional unit of the dentine-pulp complex shares the odontoblast, located in the border of the dentine pulp, with basal nuclei and organelles. The odontoblast process emerges from its apical pole. It is formed by microtubules, microfilaments and vesicles covered by membranes penetrating the dentinal tubules, isolated from the inter-tubular matrix, along the extent of the dentine. The objective of this study was to evaluate the efficacy of three staining techniques: hematoxylin-eosin, periodic acid-Schiff and Schmorl, by staining the process, from beginning to end, and compare the results with the erosion technique. Thirty human teeth were employed in the trial; after their extraction the pulp was fixated, the pieces...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulp dentine organ; Odontoblast; Odontoblast process.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000200003

A likely role for a novel PH-domain containing protein, PEPP2, in connecting membrane and cytoskeleton
Provedor de dados: Biocell
Autores: Zou,Yi; Zhong,Wenping.
PH domains (pleckstrin homology) are well known to bind membrane phosphoinositides with different specificities and direct PH domain-containing proteins to discrete subcellular apartments with assistances of alternative binding partners. PH domain-containing proteins are found to be involved in a wide range of cellular events, including signalling, cytoskeleton rearrangement and vesicular trafficking. Here we showed that a novel PH domain-containing protein, PEPP2, displayed moderate phosphoinositide binding specificity. Full length PEPP2 associated with both plasma membrane and microtubules. The membrane-associated PEPP2 nucleated at cell-cell contacts and the leading edge of migrating cells. Overexpression of PEPP2 increased membrane microviscosity,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microtubule; Membrane; Phosphoinositide; Microviscosity; Wortmannin.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300005

Edematogenic and myotoxic activities of the duvernoy's gland secretion of Philodryas olfersii from the north-east region of Argentina
Provedor de dados: Biocell
Autores: Acosta de Pérez,Ofelia; Leiva de Vila,Laura; Peichoto,María Elisa; Maruñak,Silvana; Ruíz,Raquel; Teibler,Pamela; Gay,Carolina; Rey,Laura.
Philodryas olfersii is found in South America, from Amazonas to Patagonia. It is important to characterize the venom of P. olfersii, who inhabits the North-East region of Argentina, since snake venoms are known to exhibit considerable variability in composition and biological activities. In this work, mice weighing 18-20 g (n = 4 for each experimental group) were used. For the edematogenic activity mice were injected s.c. in the right foot pad with 50 ml of solutions containing different amounts of venom, whereas the left foot pad was injected with 50 ml of PBS. Two hours after injection mice were killed by cervical dislocation and both feet were cut off and weighed individually. For the myotoxic activity mice were injected i.m. with 100 ml of solutions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Philodryas olfersii; Edematogenic activity; Myotoxic activity.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300007

Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species
Provedor de dados: Biocell
Autores: Cebral,Elisa; Carrasco,Isabel; Vantman,David; Smith,Rosita.
Exposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects of reactive oxygen species (ROS) on mouse preimplantation development, depending of the ROS-concentration and time of exposition, were studied. Two-cell embryos were incubated with 5, 10, 25 and 50 &amp;#956;M of hydrogen peroxide (H2O2) for 30 and 60 minutes of exposition and allowed to develop for 72 h to study the quality of development. The incubation with 50 &amp;#956;M2HO2 for 30 or 60 minutes, strongly inhibited the 2-cell embryo development as compared to the control (p&lt;0.001). Twenty-five &amp;#956;M2HO2 produced inhibition of blastocyst formation (p&lt;0.001) and 10 &amp;#956;M...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reactive oxygen species; Preimplantation embryogenesis; Toxicity; Mouse.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100006

Behavior and preservation of an in vitro collection of European aspen in Spain
Provedor de dados: Biocell
Autores: Martin,M.T.; Pedranzani,H.E.; Sierra de Grado,R.
An in vitro collection has been established with selected European aspen from Palencia province (Spain). Currently, this collection includes 32 high quality clones, selected for their good bearing and healthy state. Most of them belong to different discrete local populations. Populus tremula L. was propagated in proliferation Aspen Culture Medium; they required subculture every 3 months. The purpose of this study was, therefore, to select a medium which allows the maintenance of 32 clones for a period longer than 3 months without subculture and to observe the behavior of those clones in 15 different culture medium compositions. Seven nodal cutting stem explants from each clone were cultured in parallel in the different media. One and three months after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Populus tremula; Germplasm conservation; In vitro plant culture; Micropropagation; Selected clone.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100005

Effects of prenatal stress on male offspring sexual maturity
Provedor de dados: Biocell
Autores: Rodríguez,Nancy; Mayer,Nora; Gauna,Héctor F.
Prenatal stimulations have been shown to have long-term effects on at reproductive activity. We evaluated the influence of the prenatal stress on the hypothalamic-pituitary-gonad (HPG) axis in male off­springs from mothers with high number of offsprings per litter (HNL) and low number of offsprings per litter (LNL) after hypothesizing that the number of offsprings per litter may modify the effect of the prenatal stress on the HPG of adult offsprings. Pregnant Wistar rats were used for this study. Immobilization (IMO) stress was used, 30 min, 3 times per week, from the 5th to 21st day of pregnancy. The weight of adrenal and gonads, and the corticosterone (COR), testosterone (TES) and luteinizing hormone (LH) plasmatic levels were analyzed in the male...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rats; Prenatal stress; Reproduction; Corticosterone; Testosterone.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100008

Confocal laser scanning microscopy and immunohistochemistry of cerebellar Lugaro cells
Provedor de dados: Biocell
Autores: Castejón,Orlando J.
The present paper shows by means of confocal laser scanning microscopy the immunoreactivity of rat cerebellar Lugaro cells for calbindin, synapsin-I, PSD-95, GluR1, CaMKII alpha, and N-cadherin. Lugaro cells were easily characterized by their location beneath Purkinje cells. Calbindin revealed immunoreactivity in the cell body, and the axonal and dendritic processes. Synapsin-I labelled the presynaptic endings on Lugaro cells. Synapsin-I and PSD-95 immunoreactivity demonstrated the localization of presynaptic and postsynaptic endings surrounding cell soma, corresponding to afferent extrinsic and intrinsic cerebellar fibers. GluR1 immunoreactivity of the soma and cell processes indicates that Lugaro cells have functional ionotropic glutamate receptors that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calbindin; Synapsin-PSD95; GluR1; CamKII; N-cadherin.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000200003

TNFa increases in vitro migration of human HPV18-positive SW756 cervical carcinoma cells
Provedor de dados: Biocell
Autores: Hidalgo,K.; Rojas,I. G.; Penissi,A. B.; Rudolph,M. I.
TNFa has been associated with both, tumor survival and apoptosis. This cytokine is also involved in promoting cell migration during wound healing and tumorigenesis. SW756 is a HPV18-positive cervical carcinoma cell line, which has been used to study different mechanisms of cervical cancer progression. An in vitro assay of scratch wound healing onto monolayers of SW756 cells was used to assess the effect of TNFa on cell migration into a wound space. It was found that SW756 cells have the ability to migrate, but not proliferate in response to scratch wounding in a serum-free medium supplemented with TNFa. RT-PCR analysis showed that SW756 cells express TNFa mRNA when incubated in medium with and without serum. Wound closure and migration rate of SW756 cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TNFa; Migration; Carcinoma; SW756; Wound healing assay.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300008

Nitric oxide synthase activity in tissues of the blowfly Chrysomya megacephala: Fabricius, 1794
Provedor de dados: Biocell
Autores: Faraldo,A. C.; Sá-Nunes,A; Faccioli,L. H.; Del Bel,E. A.; Lello,E.
Although insects lack the adaptive immune response of the mammalians, they manifest effective innate immune responses, which include both cellular and humoral components. Cellular responses are mediated by hemocytes, and humoral responses include the activation of proteolytic cascades that initiate many events, including NO production. In mammals, nitric oxide synthases (NOSs) are also present in the endothelium, the brain, the adrenal glands, and the platelets. Studies on the distribution of NO-producing systems in invertebrates have revealed functional similarities between NOS in this group and vertebrates. We attempted to localize NOS activity in tissues of naïve (UIL), yeast-injected (YIL), and saline-injected (SIL) larvae of the blowfly Chrysomya...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NO synthase; Nitric oxide; NADPH-diaphorase; Chrysomya megacephala; Blowfly; Tissues.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000200002

High passage numbers induce resistance to apoptosis in C2C12 muscle cells
Provedor de dados: Biocell
Autores: Pronsato,Lucia; La Colla,Anabela; Ronda,Ana Carolina; Milanesi,Lorena; Boland,Ricardo; Vasconsuelo,Andrea.
Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (&lt;20) or high (&amp;gt;60) passage numbers (identified as l-C2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2O2 induction of apoptosis. We propose serially cultured C2C12 cells as a refractory model to H2O2-induced apoptosis. In addition, the data obtained in this work suggest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Over-subculturing changes; Aged muscle cultures; Mitochondrial DNA.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000100001

Localization of Sweet potato chlorotic stunt virus (SPCSV) in synergic infection with Potyviruses in sweet potato
Provedor de dados: Biocell
Autores: Nome,Claudia Fernanda; Nome,Sergio Fernando; Guzmán,Fabiana; Conci,Luis; Laguna,Irma Graciela.
Among diseases reported worldwidely for sweet potato (Ipomoea batatas (L) Lam) crop, one of the most frequent is the Sweet potato virus disease (SPVD), caused by sweet potato chlorotic stunt virus (SPCSV) and sweet potato feathery mottle virus (SPFMV) co-infection. In Argentina, there exists the sweet potato chlorotic dwarf (SPCD), a sweet potato disease caused by triple co-infection with SPCSV, SPFMV and sweet potato mild speckling virus (SPMSV). Both diseases cause a synergism between the potyviruses (SPFMV and SPMSV) and the crinivirus (SPCSV). Up to date, studies carried out on the interaction among these three viruses have not described their localization in the infected tissues. In single infections, virions of the crinivirus genus are limited to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sweet potato chlorotic stunt virus; HSP70h; In situ hybridization; Crinivirus; Sweet potato.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100003

Apoptosis as pathogenic mechanism of infection with vesicular stomatitis virus: Evidence in primary bovine fibroblast cultures
Provedor de dados: Biocell
Autores: López-Herrera,A.; Ruiz-Sáenz,J.; Góez,Y.P.; Zapata,W.; Velilla,P.A.; Arango,A.E.; Urcuqui-Inchima,S.
To determine whether fibroblasts from Blanco Orejinegro cattle, exhibit any level of resistance to infection against vesicular stomatitis virus (VSV) serotypes Indiana (VSV-I) or New Jersey (VSVNJ), 30 fibroblast cultures were phenotyped to evaluate their resistance/susceptibility. Thirty three % of Blanco Orejinegro fibroblast cultures were classified as very resistant, 50% as resistant, and 17% as susceptible to VSV-I infection, whereas 20% were classified as very resistant, 50% as resistant and 30% as susceptible to VSV-NJ infection. Therefore, there appears to be a large variation in phenotypic polymorphism among the fibroblasts to infection by VSV. To elucidate the mechanisms responsible for this diversity, we searched for a possible relationship...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vesicular stomatitis virus; Apoptosis; Resistance; Bovine fibroblasts (Source: NLM).
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200007

Oogenesis in the swamp eel Synbranchus marmoratus (Bloch, 1795) (Teleostei; synbranchidae). Ovarian anatomy, stages of oocyte development and micropyle structure
Provedor de dados: Biocell
Autores: Ravaglia,Mario Aldo; Maggese,María Cristina.
Synbranchus marmoratus (Synbranchidae), commonly known as the swamp eel, is a protogynous diandric teleost fish widely distributed throughout South America. The purpose of this work was to study the ovarian anatomy and to describe oocyte developmental stages in the swamp eel, Synbranchus marmoratus. S. marmoratus has a unique sacular ovary. It is covered by a conspicuous muscular wall, probably involved in an egg-releasing system acting as a peristaltic-like mechanism. The internal ovarian anatomy shows a U-shaped ovarian lamella delimiting a dorsal ovarian lumen. The microscopic study shows evidence of the existence of a germinal epithelium in the inner surface of the lamella, which contains germinal cells, pre-follicular cells and epithelial cells. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Synbranchidae; Ovarian anatomy; Oogenesis; Micropyle.
Ano: 2002
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452002000300003

Agrobacterium rhizogenes vs auxinic induction for in vitro rhizogenesis of Prosopis chilensis and Nothofagus alpina
Provedor de dados: Biocell
Autores: Caro,Luis A.; Santecchia,Natalia; Marinangeli,Pablo A.; Curvetto,Néstor R.; Hernández,Luis F.
The induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (BTMm) containing half salt concentration of macronutrients and 0.05 mg.L-1 benzilaminopurine (BAP). After 30 days, microshoots with 2-4 leaves were selected and cultured in BTMm-agar in presence or abscense of Ar and in combination with IBA. For N. alpina, the apical shoots with the first 2 true leaves, from 5 weeks old seedlings, were cultured in the abovementioned...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agrobacterium rhizogenes; Algarrobo; Hairy roots; Indole-3-butyric acid; Nothofagus nervosa; Prosopis chilensis; Raulí; Rooting.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300002

Mechanisms involved in the cytotoxic effects of berberine on human colon cancer HCT-8 cells
Provedor de dados: Biocell
Autores: XU,Li-na; LU,Bi-nan; HU,Ming-ming; XU,You-wei; HAN,Xu; QI,Yan; PENG,Jin-yong.
Berberine, a constituent of some traditional Chinese medicinal plants, has been reported to have cytotoxicity effects on different human cancer cell lines. There is no available information about the effects and mechanism of action of berberine on human colon cancer cell line HCT-8. In this paper, the cytotoxicity of berberine on HCT-8 cancer cells was investigated by MTT assay, fluorescence microscopy and flow cytometry analysis. Our data revealed that berberine could significantly inhibit the growth of HCT-8 cells in a dose- and time-dependent manner. Morphology of apoptotic cells was studied with acridine orange/ethidium bromide staining. The concentrations of lactate dehydrogenase and both acid and alkaline phosphatases were significantly increased in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Cell cycle arrest; Death receptor pathway; Mitochondrial pathway; Natural product.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300003

Abstracts from the Twelfth's Multidisciplinary Workshop (Décima segunda Jornada Multidisciplinaria): December, 2010 Buenos Aires, Argentina
Provedor de dados: Biocell
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000100007

Structure of the kidney of Bufo arenarum: Intermediate segment, distal tubule and collecting tubule
Provedor de dados: Biocell
Autores: Farías,Alejandro; Hermida,Gladys Noemí; Fiorito,Luisa Eleonora.
The ultrastructure of the intermediate segment (IS), distal tubule and collecting tubule (CT) of the south american toad Bufo arenarum, was studied by light and transmission electron microscopy. The IS is composed of cubical ciliated cells which propel the urine along the renal tubule. The distal tubule is divided into two portions: the early distal tubule (EDT) and the late distal tubule (LDT). The EDT is characterized by only one type of cells with well developed basolateral interdigitations and numerous elongated mitochondria, which are oriented normal to the basal surface. The "macula densa - like" is a specialized zone of the EDT in contact with the vascular pole, where cells are more tightly packed than in the rest of the tubule. The LDT shows two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amphibia; Kidney; Ultrastructure; Renal tubules.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000100003

Sequential expressions of Notch1, Jagged2 and Math1 in molar tooth germ of mouse
Provedor de dados: Biocell
Autores: Borkosky,Silvia S.; Nagatsuka,Hitoshi; Orita,Yorihisa; Tsujigiwa,Hidetsugu; Yoshinobu,Junko; Gunduz,Mehmet; Rodriguez,Andrea P.; Missana,Liliana R.; Nishizaki,Kazunori; Nagai,Noriyuki.
The Notch signaling pathway is an evolutionary conserved mechanism that plays an important role in cell-cell communication and cell fate in a wide range of tissues. The mammalian family of Notch receptors consists of 4 members: Notch1/2/3/4. The Notch ligand family consists of 5 members: Delta1/3/4 and Jagged1/2. Math1 encodes a murine basic helix-loop-helix (bHLH) transcription factor that acts as positive regulator of cell differentiation. Recently, links between Notch and Math1 pathways were demonstrated in various tissues. Expression of Notch1, Jagged2 and Math1 were analyzed in the mouse molar tooth germ during embryonic stage (E) 13 and E15 and during postnatal stage (PN) 1, PN3, PN5, PN10 and PN14 by using in situ hybridization. Positive Notch1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Notch signaling; Odontogenesis; Cell differentiation; Molar morphogenesis.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000300006

RNA fingerprinting using RAP-PCR identifies an EBAF homologue mRNA differentially expressed in rat oviduct
Provedor de dados: Biocell
Autores: Valdecantos,Pablo A.; Argañaraz,Martín E.; Abate,Carlos M.; Miceli,Dora C.
As a step towards the identification of genes preferentially expressed in the oviduct during early rat embryo development, we isolated a cDNA fragment (Pr14) by using RNA arbitrarily primed PCR (RAP-PCR), being its expression restricted to oviduct and uterus; its mRNA is mainly expressed in oviduct during late luteal phase and early pregnancy. This fragment is 100% identical to a rat DNA sequence (Accession No. NW_047400) downstream the terminal exon of a Rattus norvegicus gene (Locus Link Accession No. LOC289316) similar to ebaf (endometrial bleeding-associated factor), a novel member of the Transforming Growth Factor superfamily. Northern analyses showed that this sequence hybridizes with 2.9 kb and 4.1 kb mRNAs in early pregnant rat oviducts. However,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Differential gene expression; Oviduct; RNA fingerprint; CDNA.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000300005

Leaf surfaces of Gomphrena spp. (Amaranthaceae) from Cerrado biome
Provedor de dados: Biocell
Autores: Carvalho,Suzane Margaret Fank-de-; Gomes,Misléia Rodrigues de Aguiar; Silva,Pedro Ítalo Tanno; Báo,Sônia Nair.
The leaf structure and micromorphology characterize plant species and reflex its interactions with the environment. Leaf epidermis sculptures aid high transpiration plants on light reflection. The form and distribution of epicuticular wax crystalloids are important to characterize the surface. Aiming to know the micromorphology and the ultrastructure of G. arborescens, G. pohlii and G. virgata, leaves of these Cerrado native species were collected in Brasília, Distrito Federal, Brazil, at the Olympic Center of the Universidade de Brasília and at Reserva Ecológica do Roncador. Leaves of G. globosa, an Indian native species, were also studied for comparison. Leaves were fractionated, fixed and treated for observation under optical and scanning electron...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomy; Epicuticular wax; Platelets; Trichomes; Stomata.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000100004

Isolation of epithelial cells, villi and crypts from small intestine of pigeons (Columba livia)
Provedor de dados: Biocell
Autores: Mac Donal,Oscar; Chediack,Juan G.; Caviedes-Vidal,Enrique.
The isolation of viable enterocytes, villi and crypts from the small intestine of a feral bird (Columba livia) is important for performing physiological experiments in ecologically relevant processes of membrane transport. The effectiveness of mechanical disruption, enzymatic digestion and chelating agents were compared. The objectives were to isolate enterocytes, villi and crypts from the small intestine of young pigeons; to evaluate the viability of the isolated intestinal epithelial cells isolated; and to verify the integrity of enterocytes by biochemical features. Enzymatic and mechanical methods yielded both elongated columnar and spherical cells. With the chelating method villi and crypts were obtained. All methods produced a high yield of intestinal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterocyte; Villus; Crypt; Isolation methods; Avian gut.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000300002

Probiotics enhance the recovery of gut atrophy in experimental malnutrition
Provedor de dados: Biocell
Autores: Dock,Diana B.; Aguilar-Nascimento,José E.; Latorraca,Marcia Q.
AIM: The aim of this study was to evaluate the effect of probiotics on the recovery of the bowel atrophy induced by malnutrition in rats. METHODS: Twenty-and-six Wistar rats (200-250g) were fed with either a normoproteic (sham group, n=6) or a free-protein diet (n=20) during 12 days. Twelve malnourished rats were randomized to recover during 15 days with either a hydrolyzed diet (control group, n=6) or the same diet enriched with probiotics (Streptococcus thermophilus and Lactobacillus helveticus; probiotic group, n=6). RESULTS: Probiotic group showed similar gain of body, liver and bowel weight than controls. At the jejunum, both the villus height (383±49 vs. 321±46mm; p=0.04) and crypt depth (157±31 vs. 125±10mm; p=0.04) were greater in probiotic group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Probiotics; Malnutrition; Small bowel; Colon; Mucosa.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000200003

Behavior of mesenchymal stem cells stained with 4&amp;', 6-diamidino-2-phenylindole dihydrochloride (DAPI) in osteogenic and non osteogenic cultures
Provedor de dados: Biocell
Autores: Ocarino,N.M.; Bozzi,A.; Pereira,R.D.O.; Breyner,N.M.; Silva,V.L.; Castanheira,P.; Goes,A.M.; Serakides,R.
4&amp;', 6-diamidino-2-phenylindole dihydrochloride (DAPI) is a DNA dye widely used to mark and trace stem cells in therapy. We here studied the effect of DAPI staining on the behavior of mesenchymal stem cells cultured in either a control, non-osteogenic medium or in an osteogenic differentiation medium. In the control medium, the number of stem cells/field, as well as the number of fluorescent cells/field increased up to the sixth day in both control and DAPI-treated cultures. Afterwards, both the number of fluorescent cells and their fluorescence intensity decreased. Control cells were fusiform and with some long extensions that apparently linked them to neighboring cells, while DAPI-treated cells were mostly round cells with fine and short extensions....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow; Cell cultures; Rat; Osteogenic differentiation.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200003

Testosterone production and spermatogenic damage induced by organophosphorate pesticides
Provedor de dados: Biocell
Autores: Contreras,H.R.; Paredes,V.; Urquieta,B.; Del Valle,L.; Bustos-Obregón,E.
ParathionÒ is an organophosphorate pesticide amply used in agriculture. Many alterations induced by organophosphorate pesticides have been described, such as: cytogenetic alterations in germinal cells, oligozoospermia and teratozoospermia in the mouse. The effect of ParathionÒ, both pure (PP) and commercial (PC), on mouse interstitial cell testosterone production was evaluated in vivo and in vitro. Male mice were intraperitoneally injected with a single dose of 1/3 LD50 of ParathionÒ, both PP and PC. The animals were sacrificed at 1, 8 and 40 days post injection to evaluate the impact of disrupting testosterone production on spermatogonia, spermatocytes and elongated spermatids. The plasma testosterone was assayed by standard radioimmunoanalysis. The same...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Steroidogenesis; Spermatogenesis; Pesticide; ParathionÒ.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000300002

Nuclear pores in luteal cells during pregnancy and after parturition and pup removal in the rat: A freeze-fracture study
Provedor de dados: Biocell
Autores: Cavicchia,Juan Carlos; Guembe,Gustavo; Fóscolo,Mabel.
In a previous paper we described a pronounced increase of apoptotic nuclei in rat corpus luteum of pregnancy whose programmed chromatin degeneration was induced by the progesterone antagonist mifepristone. Those observations encouraged us to study the apoptotic nuclear membrane during pregnancy and after parturition and pup removal, by using a freeze-fracture technique which allows us to observe 'en face' the nuclear envelop and also permits nuclear pore counting. This study was complemented with the TUNEL assay (TdT-mediated dUTP nick-end labelling). Changes in nuclear pores during pregnancy begin with an intense reduction in number but still showing an even distribution on the nuclear membrane, never forming aggregations sharply separated from pore-free...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Corpus luteum; Nuclear envelope; Nucleocytoplasmic transport.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000200004

Isolation and biological characterization of a basic phospholipase A2 from Bothrops jararacussu snake venom
Provedor de dados: Biocell
Autores: Maruñak,S.L.; Leiva,L; Garcia Denegri,M.E.; Teibler,P; Acosta De Pérez,O.
A phospholipase A2 has been isolated from Bothrops jararacussu venom from snakes that inhabit the northeast region of Argentina. The present study describes in vivo and in vitro biological activities of phospholipase A2 from B. jararacussu as well as isolation details of its. Venom was obtained by milking of adult snakes which were housing in wood reptile cages of varying dimensions in heated (20-30ºC) rooms. Snakes received a weekly diet of mice and water was available ad libitum for drinking and soaking. The enzyme was purified by gel filtration on a Sephadex G-75 column followed by ion exchange chromatography on a SP-Sephadex C25 column. The major peak belonging to proteins was retained in the cation exchanger and then eluted using a concentration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops jararacussu; Phospholipase A2; Isolation.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300001

Taxus globosa S. cell lines: Initiation, selection and characterization in terms of growth, and of baccatin III and paclitaxel production
Provedor de dados: Biocell
Autores: Barradas-Dermitz,Dulce Ma.; Hayward-Jones,Patricia M.; Mata-Rosas,Martín; Palmeros-Sánchez,Beatriz; Platas-Barradas,Oscar B. J.; Velásquez-Toledo,Rodolfo F.
Of the initial six cell lines originating from explants of Taxus globosa, or Mexican yew (stem internode, leaves and meristematic tissue), three were selected for their microbial and oxidation resistance, two from leaves and the other from stem internode. A study of their behavior, both in terms of cell growth, and of baccatin III and paclitaxel production, was developed in suspension cultures with an initially standardized biomass (fresh weight 0.23 g/L) using modified Gamborg's B5 medium, and an elicitor (methyl jasmonate), on either the first or seventh day of culture, at several levels (0, 0.1, 1, 10, 100 &amp;#956;M). In most of the conditions used, the three cell lines showed growth associated baccatin III production. The cell line from stem...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth-associated metabolites; Methyl jasmonate; Paclitaxel precursor; Secondary metabolism; Suspension cell culture.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000100001

Mitochondrial apoptotic pathways
Provedor de dados: Biocell
Autores: Mohamad,Nora; Gutiérrez,Alicia; Núñez,Mariel; Cocca,Claudia; Martín,Gabriela; Cricco,Graciela; Medina,Vanina; Rivera,Elena; Bergoc,Rosa.
Apoptosis or programmed cell death (PCD) is a physiological process characteristic of pluricellular organisms leading to self-destruction of the cell. It is therefore involved in development, homeostasis and host defense. However, a significant difference has been shown between mammalian cell apoptosis and non-mammalian cell apoptosis: mitochondria are implicated only in the former. Execution of PCD includes the release of several proapoptotic proteins from the intermembrane space of mitochondria. They could exert their actions through a caspase dependent as well as a caspase independent way. On the other hand, regulation of PCD is mainly given by the Bcl-2 family members, which are in turn essentially regulated by activation of death receptors and/or DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Mitochondria; Bcl-2 family; Cytochrome-c.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200001

Effects of filgrastim on granulopoietic cells of mice pretreated with methotrexate
Provedor de dados: Biocell
Autores: Barrios,Lilian; Poletti,Oscar Héctor.
We have evaluated the effect of filgrastim on proliferation and differentiation activity of granulopoietic cells in mice pretreated with methotrexate. Filgrastim was injected daily, from day 8 to 28 after cytotoxic agent administration. The granulopoiesis changes were measured by assessment of GM-CFU cells content, marrow and spleen granuloid cells pool as well as circulating neutrophils. In MTX pretreated mice, bone marrow GM-CFU oscillating values were higher than normal values, but these changes were not followed by high proliferative activity in granuloid precursor cell compartment. After MTX treatment, filgrastim administration was unable to stimulate marrow granulopoiesis as observed in normal mice. In the spleen, MTX led to dramatic changes in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methotrexate; Filgrastim; Granulopoiesis; Bone marrow; Spleen.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000100002

Chromosome comparison between populations of the collared peccary, Tayassu tajacu, raised in captivity
Provedor de dados: Biocell
Autores: Carvalho de Souza,Patrícia; Salim Khayat,André; Chamon Seligmann,Igor; Rodríguez Burbano,Rommel Mario.
The collared peccary (Tayassu tajacu) is widely distributed over the American continent, being found from the south of the USA to the north of Argentina. In Brazil, it is spread all over the country, being one of the potential species to be raised in captivity. Therefore, the cytogenetic techniques could be a potential tool for reproductive monitoring of animals raised in captivity, mainly when destined for commercial purposes. This study had the objective of determining the chromosome number of two populations raised in captivity and characterizing them by GTG banding. For this purpose, an analysis was made of mitotic metaphases obtained from lymphocyte cultures made from blood samples of 11 animals, six of which from the Northeast and five from the North...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytogenetics; GTG Banding; Tayassuidae; Suidae; Pigs.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200007

Uterine mast cells: A new hypothesis to understand how we are born
Provedor de dados: Biocell
Autores: Rudolph,M. Isolde; Rojas,I. Gina; Penissi,Alicia B.
Birth is the result of complex, well-defined, and coordinated events, that are tightly regulated by endocrine, nervous, and immune responses, and take place primarily in the female reproductive tract. Various mechanisms and mediators involved in pregnancy, labor, and delivery, are highly conserved among different mammalian species and mast cells emerge as potential and crucial participants in these processes, as it is discussed in this review.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Uterine Mast Cells; Labor; Myometrium Contractions; Cervical Ripening.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000100001

Microanatomical characteristics of marginal ommatidia in three different size-classes of the semi-terrestrial isopod Ligia exotica (Crustacea; Isopoda)
Provedor de dados: Biocell
Autores: Keskinen,Essi; Takaku,Yasuharu; Meyer-Rochow,Benno; Hariyama,Takahiko.
The aims of this paper have been (a) to characterize marginal ommatidia from different eyeregions through a detailed description of their distinct ultrastructural features in three different size-classes ofL. exotica, and (b) to compare microanatomical characteristics of the marginal ommatidia with those of ommatidia of the same eye, but located further centrally. On the basis of transverse as well as longitudinal sections we conclude that new ommatidia are added from a crescentic dorso-anterio-ventral edge of the eye and that maturing ommatidia go through a sequence in which originally the nuclei of cone -, pigment-, and retinula cells are arranged in three separate layers. At the beginning of the microvillar development, the organization of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crustaceans; Eye; Retina; Vision; Growth; Moulting; Ultrastructure.
Ano: 2002
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452002000300006

Inhibition of focal adhesion kinase by antisense oligonucleotides enhances the sensitivity of breast cancer cells to camptothecins
Provedor de dados: Biocell
Autores: Satoh,Taroh H.; Surmacz,Tatiana A.; Nyormoi,Okot; Whitacre,Cecilia M.
This study shows a strong association between cell attachment to substratum and activation of b1-integrin-signaling with resistance to the camptothecin derivative topotecan (TPT) in breast cancer cells. We propose a mechanistic-driven approach to sensitize the cells to camptothecins. ZR-75-1 anchorage-dependent breast cancer cell line, its derivative 9D3S suspension cells (9D3S-S), and 9D3S cells attached to fibronectin-coated plates (9D3S-A) were treated with TPT (1 mM) or CPT-11 (40 mM) for 48 h. Programmed cell death (PCD), as shown by poly(ADP-ribose) polymerase (PARP), pro-caspase-3 and pro-caspase-9 cleavage, was observed in 9D3S-S cells but not in ZR-75-1 or 9D3S-A cells. Because p125 focal adhesion kinase (FAK) is a transducer in the b1-integrin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Focal adhesion kinase; Drug resistance; Camptothecins; Breast cancer.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000100006

Ubisch bodies and pollen ontogeny in Oxalis articulata Savigny
Provedor de dados: Biocell
Autores: Rosenfeldt,Sonia; Galati,Beatriz G.
The correlation between the ontogeny of Ubisch bodies and pollen development in Oxalis articulata was studied with Transmission Electron Microscopy (TEM). The ultrastructural changes observed during the different stages of development in the tapetal cells are related to Ubisch bodies, sporopollenin and pollen-kitt formation. The pro-orbicules have the appearance of lipid globuli and their formation is related to the endoplasmic reticulum of rough type (ERr). The lipid globules or pro-orbicules disappear in the mature Ubisch bodies, and the places that they occupied remain free of contents or with pollen-kitt.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pollen; Ubisch bodies; Ontogeny; Utrastructure; Oxalis.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300004

Morphological and histochemical study of the masseter muscle after occlusal alteration
Provedor de dados: Biocell
Autores: Mardegan Issa,João Paulo; Tiossi,Rodrigo; Mizusaki Iyomasa,Mamie.
The aim of this study was to evaluate alterations in the masseter muscle of 30 male guinea-pigs submitted to occlusal alteration. The animals were divided into 2 equal groups, the control group (C) only submitted to surgical stress, and the occlusal altered group (T) submitted to teeth extraction. Each group was subdivided into 3 groups, with 5 animals, for the following studies: macroscopy and vessels distribution, light microscopy and histochemical analysis, with animals' perfusion 2 months after surgery. Data were submitted to statistical analysis. Macroscopically, an anteroposterior orientation of the muscular fibers was found and statistical difference between C and T groups in width (p&lt;0.05). Microscopically, C and T groups showed polygonal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Masseter muscle; Morphology; Histology; Histochemistry.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300003

Structural analysis of flagellar axonemes from inner arm dynein knockdown strains of Trypanosoma brucei
Provedor de dados: Biocell
Autores: Zukas,Randi; Chang,Alex J.; Rice,Marian; Springer,Amy L.
Trypanosoma brucei is a protozoan flagellate that causes African sleeping sickness. Flagellar function in this organism is critical for life cycle progression and pathogenesis, however the regulation of flagellar motility is not well understood. The flagellar axoneme produces a complex beat through the precisely coordinated firing of many proteins, including multiple dynein motors. These motors are found in the inner arm and outer arm complexes. We are studying one of the inner arm dynein motors in the T. brucei flagellum: dynein-f. RNAi knockdown of genes for two components of dynein-f: DNAH10, the a heavy chain, and IC138, an intermediate chain, cause severe motility defects including immotility. To determine if motility defects result from structural...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flagellar motility; Transmission electron microscopy; Central pair.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300006

Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver
Provedor de dados: Biocell
Autores: Martín,Carlos A.; Surur,José Miguel; García,Marcela N.; Corrons,Félix; Badrán,Amado F.
Variations in DNA synthesis (DNAs) and Nucleolar Organizer Regions (NORs) were studied in the littoral cell population from regenerating liver of C3HS inbred mice standardized for periodicity analysis. Immunohistochemical detection of Bromodeoxyuridine (BrdU) with a monoclonal antibody and silver staining of NORs (AgNORs) were assessed by means of a digital image analysis system in histological sections. Tissue samples were obtained every four hours from the 30th to the 54th hours after a partial hepatectomy. The results showed, in both parameters, a gradual increment of the values during the period studied, with highest values (DNAs 107.1 ± 16.1 SE; AgNORs 77.3 ± 3.4 SE) located at 16:00/54 Time of Day / Hours Post- Hepatectomy (TD/HPH), which were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver regeneration; Sinusoid littoral cell; Bromodeoxyuridine; AgNOR; Immunohistochemistry.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000100005

Endogenous ADP-ribosylation of eukaryotic elongation factor 2 and its 32 kDa tryptic fragment
Provedor de dados: Biocell
Autores: Ergen,,Kivanç; Bektas¸,Muhammet; Gökçe,Sina; Nurten,Rüstem.
Eukaryotic elongation factor 2 (eEF-2) can undergo ADP-ribosylation in the absence of diphtheria toxin. The binding of free ADP-ribose and endogenous transferase-dependent ADP-ribosylation were distinct reactions for eEF-2, as indicated by different findings. Incubation of eEF-2 tryptic fragment 32/33 kDa (32F) with NAD was ADP-ribosylated and gave rise to the covalent binding of ADP-ribose to eEF-2. 32F was revealed to be at the C-terminal by Edman degradation sequence analysis. In our study, the elution of 32F from SDS-PAGE was ADP-ribosylated both in the presence and absence of diphtheria toxin. These results suggest that endogenous ADP-ribosylation of 32F might be related to protein synthesis. This modification appears to be important for the cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eukaryotic elongation factor 2; Endogenous ADP-ribosylation; Protein synthesis; Trypsin digestion; Diphtheria toxin.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100007

Ultrastructural changes of the olfactory bulb in manganese-treated mice
Provedor de dados: Biocell
Autores: Villalobos,Virginia; Bonilla,Ernesto; Castellano,Alan; Novo,Ernesto; Caspersen,Ralph; Giraldoth,Debora; Medina-Leendertz,Shirley.
The effect of manganese toxicity on the ultrastructure of the olfactory bulb was evaluated. Male albino mice were injected intraperitoneally with MnCl2 (5 mg/Kg/day) five days per week during nine weeks. The control group received NaCl (0.9%). The olfactory bulbs of five mice from each group were processed for transmission electron microscopy after 2, 4, 6 and 9 weeks of manganese treatment. On week 2, some disorganization of the myelin sheaths was observed. After 4 weeks, degenerated neurons with dilated cisternae of rough endoplasmic reticulum and swollen mitochondria appeared. A certain degree of gliosis with a predominance of astrocytes with swollen mitochondria, disorganization of the endomembrane system, dilation of the perinuclear cisternae and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metal toxicity; Toxicity; Neurodegeneration; Electron microscopy.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000300007

The Darwin Initiative and the whitefish Chirostoma estor estor: A link between aquaculture, biodiversity and rural livelihoods
Provedor de dados: Biocell
Autores: Ross,Lindsay G.; Martínez-Palacios,Carlos A.; Rodríguez De Sousa,Maria Luisa; Campos-Mendoza,Antonio.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chirostoma; México; Biodiversity; Rural livelihoods; Darwin Initiative.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100014

Apoptogenic effect of the lipophilic o-naphthoquinone CG 10-248 on rat hepatocytes: light and electron microscopy studies
Provedor de dados: Biocell
Autores: Lopez,Lidia M.; Dubin,Marta; Carrizo,Patricia H.; Burgos,Mario H.; Pellegrino de Iraldi,Amanda; Stoppani,Andrés O. M.
CG 10-248 (3,4-dihydro-2,2-dimethyl-9-chloro-2H-naphtho&amp;#091;1,2b&amp;#093;pyran- 5,6-dione; CG-NQ), a b-lapachone analogue, modified the ultrastructure of rat hepatocytes, as demonstrated by light and electron microscopy. After 4 h incubation with 100 µM CG-NQ, the following effects were observed: (a) nuclear chromatin condensation; (b) chromatin fragmentation; (c) displacement of mitochondria, concentrated around the nucleus; (d) disruption or expansion of mitochondrial outer or inner membranes, respectively; (e) displacement and alteration of endoplasmic reticulum (rough and smooth); (f) decrease of microvilli; (g) blebbing of plasma membrane and production of apoptotic bodies formed by folding of plasma membrane fragments around mitochondria or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; O-naphthoquinones; Hepatocytes; Chromatin Alteration; Mitochondrial Swelling Membrane; Blebs.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000200007

p53-Rb signaling pathway is involved in tubular cell senescence in renal ischemia/reperfusion injury
Provedor de dados: Biocell
Autores: Li,Kailong; Du,Xiaolan; He,Yani; Zhao,Lin; Yang,Jvrong; Song,Ruihua; Chen,Lin.
Objective: To investigate the course of tubular cell senescence and expressions of p53, p21, and Rb during the late phase of ischemia/reperfusion (IRI) in the kidney, and assess the effects of the p53-Rb pathway on tubular cell senescence. Methods: Experimental models of unilateral renal IRI were used in p53(+/+) and p53(-/-) mice. Histological changes at the tubular level, progress of cell senescence, and the expression of Rb, p21, and/or p53 proteins in tubular cells were studied at different moments in time after IRI. Results: Chronic tubulointerstitial fibrosis was much more severe and widely distributed in IRI kidneys of p53(+/+) mice in later stages than in earlier stages. Senescent tubular cells were significantly increased at 3 and 6 months after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell senescence; Cell cycle regulatory protein; Ischemia/reperfusion injury; Kidney.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000200003

Plant regeneration after long term callus culture in clones of Asparagus officinalis L.
Provedor de dados: Biocell
Autores: Pontaroli,A. C.; Camadro,E. L.
Callus growth and plant regeneration from long-term callus cultures were studied in two elite clones of Asparagus officinalis cv. Argenteuil, to establish a suitable protocol for a prospective in vitro selection program. Callus initiation and growth was evaluated on MS medium with 3% sucrose, 0.9% agar, 1 mg.l-1 kinetin, and three levels of 2,4-D. The highest callus relative growth was obtained on medium with 1.5 mg.l-1 2,4-D and 1 mg.l-1 kinetin. Shoot primordia (SP) induction from &gt;18-months-old calluses was evaluated on several media; the highest percentage of SP induction (89%) and average number of SP per callus (8.6) were obtained with clone &amp;#8216;265' on MS medium with 5 mg.l-1 2iP, 1 mg.l-1 IAA, 3% sucrose and 0.9% agar. The highest...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Callus; Shoot primordia induction; Rooting; Medium x genotype interactions.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300009

Influence of 2-methoxyestradiol on MCF-7 cells: An improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels
Provedor de dados: Biocell
Autores: Joubert,Annie; Marais,Sumari; Maritz,Christine.
Proteins of the B-cell lymphoma 2 family are crucial for the regulation of apoptosis. B-cell lymphoma 2-associated X is a pro-apoptotic protein, while B-cell lymphoma 2 protein opposes apoptosis. The influence of 1 &amp;#956;M 2-methoxyestradiol was investigated on the expression levels of these two proteins in MCF-7 cells. 2-Methoxyestradiol exposure did not influence B-cell lymphoma 2 protein expression levels after 24 h of exposure. In contrast, B-cell lymphoma 2-associated X protein levels were significantly reduced. An improved differential interference contrasting technique revealed compromised cell density and the presence of a mitotic block in exposed cells. The study proposes that the influence of 2-methoxyestradiol on the expression of these...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Breast carcinoma; Mitosis; Apoptosis.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000100007

Incidence of sperm-tail tyrosine phosphorylation and hyperactivated motility in normozoospermic and asthenozoospermic human sperm samples
Provedor de dados: Biocell
Autores: Yunes,Roberto; Doncel,Gustavo F.; Acosta,Anibal A.
Our objective was to study the incidence of sperm-tail phosphotyrosine immunoreactivity in normozoospermic and asthenozoospermic human sperm samples, its association with sperm motion parameters, particularly hyperactivated motility, and its potential involvement in the pathogenesis of asthenozoospermia. The work was conducted as a prospective experimental study in the Sperm Biology and Andrology laboratories of the Jones Institute, a medical school-based fertility center. The study subjects were healthy fertile male donors (normozoospermic samples) and infertile patients (asthenozoospermic samples) attending the center. Recently ejaculated semen samples were washed twice to eliminate seminal plasma and a swim-up was performed to select the motile...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sperm motility; Hyperactivation; Asthenozoospermia; Tyrosine-phosphorylation; Protein-tyrosine kinase.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000100004

Differentiation and morphogenesis of Triatoma infestans (Klug 1834) female gonads: I - Post embryonic development
Provedor de dados: Biocell
Autores: Ibañez de Barrett,Cecilia I.; Bozzini,Juan Pablo; Mariano de Bozzini,Marta.
The post-embryonic development of the female gonads in Triatoma infestans (Hemiptera, Heteroptera), insects of importance in health affairs as harbors and vectors of different tripanosomatidea flagellates, is presented in a complete follow-up since insect hatches from the egg up to the last molt in the fifth instar stage. The detailed description of the morphological changes which occur in each instar as well as careful measurements evaluating its size increase have been analyzed by stereomicroscopy, phase contrast, dark field, and oblique illumination, in order to optimize the observations as well the photographic register of gonad morphology and structure. The analysis was performed on gonad specimens obtained from broods no less than twenty (20) nymph...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovary; Development; Hemiptera; Insect Nymph.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000300002

MDCK cells express serotonin-regulable 11ß-hydroxysteroid dehydrogenase type 2
Provedor de dados: Biocell
Autores: Zallocchi,Marisa L.; Damasco,Maria C.; Calvo,Juan C.; Lantos,Carlos P.; Matkovic',Laura B.
Prior to this work, we found that adrenal as well as extra-adrenal factors activate the response of renal 11ß-hydroxysteroid dehydrogenase 2 to stressful situations. These results -showing ways through which the organism hinders the pathological occupation of mineralocorticoid receptors by glucocorticoids leading to sodium retention and hypertension- prompted the present study on the nature of the above-mentioned extraadrenal factors. Serotonin was chosen because of its properties as a widely distributed neurohormone, known to interact with glucocorticoids at many sites, also exhibiting increased levels and effects under stressful situations. We studied serotonin effects on 11b-hydroxysteroid dehydrogenase 2 activity in a cell line derived from distal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serotonin; 11b-HSD2; Stress hormones; Mineralocorticoid; Glucocorticoid.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000300007

Biochemical-molecular markers in unilateral ureteral obstruction
Provedor de dados: Biocell
Autores: Manucha,Walter.
Congenital obstructive nephropathy is the primary cause of end-stagerenal disease in children. Rapid diagnosis and initiation of the treatment are vital to preserve function and/or to slow down renal injury. Obstr uctive uropathy effects-decline in the plasmatic renal flow and glomerular filtrationrate, interstitial infiltrate of leukocytes, significant decrease of the urine concentration, loss of the capacity to concentrate urine as well as fibrosis and apoptosisare a consequence of a variety of factors that work in complex ways and are still not fully understood. Mediators as angiotensin II, transforming growth factor-b (TGF-b) and nitric oxide (NO) have been implicated in congenital obstructive nephropathy. The renin-angiotensin system is regulated in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Unilateral ureteral obstruction; Interstitial fibrosis; Nitric oxide; Angiotensin II; Oxidative stress.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100001

Immunohistochemistry of GluR1 subunits of AMPA receptors of rat cerebellar nerve cells
Provedor de dados: Biocell
Autores: Castejón,Orlando J.; Dailey,Michael E.
The localization of GluR1 subunits of ionotropic glutamate receptors in the glial cells and inhibitory neurons of cerebellar cortex and their association with the climbing and parallel fibers, and basket cell axons were studied. Samples of P14 and P21 rat cerebellar cortex were exposed to a specific antibody against GluR1 subunit(s) of AMPA receptors and were examined with confocal laser scanning microscopy. GluR1 strong immunoreactivity was confined to Purkinje cell and the molecular layer. Weak GluR1 immunoreactivity was observed surrounding some Golgi cells in the granule cell layer. Intense GluR1 immunoreactivity was localized around Purkinje, basket, and stellate cells. Purkinje cells expressed strong GluR1 immunoreactivity surrounding the cell body,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutamate receptors; Glia; Neurons; Confocal microscopy.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200001

Studies on the reproductive and developmental biology of Cichlasoma dimerus (Percifomes, Cichlidae)
Provedor de dados: Biocell
Autores: Pandolfi,Matías; Cánepa,Maximiliano M.; Meijide,Fernando J.; Alonso,Felipe; Rey Vázquez,Graciela; Maggese,M. Cristina; Vissio,Paula G.
Many characteristics of the South American teleost fish Cichlasoma dimerus (body size, easy breeding, undemanding maintenance) make it amenable to laboratory studies. In the last years, many of the fundamental aspects of its reproductive and developmental biology have been addressed in our laboratory. Rather recently, the immunohistochemical localization of pituitary hormones involved in reproduction and in background color adaptation has been described in both adult and developing individuals, and the role of FSH in ovarian differentiation has been established. These findings have been correlated with mapping of some of their brain-derived controlling hormones. The latter include brain-derived gonadotropins which were shown to be active in vitro in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reproduction; Early development; Neuroendocrinology; Brain; Pituitary; Teleosts.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000100001

Synergism of flavonoids with bacteriostatic action gainst Staphylococcus aureus ATCC 25 923 and Escherichia coli ATCC 25 922
Provedor de dados: Biocell
Autores: Alvarez,María De Los A.; Debattista,Nora B.; Pappano,Nora B.
In our previous studies, bacteriostatic action of flavonoids against Staphylococcus aureus ATCC 25 923 and Escherichia coli ATCC 25 922 was demostrated. In the present work synergism of their combinations in order to improve the bacteriostatic action against the same microorganisms was determined. The experiences were made in nutritive broth, maintaining constant one drug concentration (20 µg/ml) and increasing the other one. A turbidimetric kinetic method was used and by means of a mechanism previously proposed, the minimal inhibitory concentrations (MIC´s) of each flavonoid combination were determined. The MIC´s for assayed combinations against S. aureus were: variable morin - constant rutin: 157.44 µg/ml and variable quercetin - constant morin: 29.9...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Synergism; Flavonoids; Bacteriostatics; MIC.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100006

DNA injury induced by 5-aminouracil and caffeine in G2 checkpoints path of higher plant cells
Provedor de dados: Biocell
Autores: Del Campo,A.; Bracho,M.; Marcano,L.; Guíñez,J.; De la Torre,C.
This work evaluated the qualitative and quantitative cellular changes induced by treatment with 5-aminouracil (5-AU) and a combination of 5-AU and caffeine in plant cells in relation to DNA damage, repaired damage, and residual damage. As biological material, Allium cepa L. root tips were used, grown in filtered water, in darkness, with aeration at constant temperature of 25 °C ± 0.5. Cell populations were synchronized using 5 mM caffeine in order to study the effects of 5-AU and caffeine/5-AU combined treatment on the DNA content and their incidence in the entrance to mitosis. The results showed a delay in the G2 period due to induced DNA damage by the 5-AU and caffeine/5-AU combined treatment, shown by aberrant metaphases, anaphases and telophases. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5-aminouracil; G2 checkpoints; Caffeine.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200003

Novel neurotrophic factor secreted by amniotic epithelial cells
Provedor de dados: Biocell
Autores: Venkatachalam,Sankar; Palaniappan,Tamilselvi; Jayapal,Prem Kumar; Neelamegan,Sridharan; Rajan,Sridhar Skylab; Muthiah,Vijaya Prakash Krishnan.
By virtue of expressions of glial and neural surface markers and capability of neurotransmitter metabolism, amniotic epithelial cells are considered as candidate cell type for transplantation strategies to treat neurological disorders. Previously, we have reported neurotrophism exhibited by human amniotic epithelial cells when transplanted after spinal cord injury in bonnet monkeys. Amniotic epithelial cells were believed to secrete an "Epidermal Growth Factor (EGF) - like" factor and exact identification was not made. At this juncture, through the present study it was found that, chicken neural retinal cells when grown alone failed to survive and contrarily when either co-cultured with chicken amniotic epithelial cells / cultured in amniotic epithelial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuroprotection; Neural retina development; FGF-2; Neurotrophism; Placental stem cells.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200002

Fine structural analysis of the epithelial cells in the hepatopancreas of Palaemonetes argentinus (Crustacea, Decapoda, Caridea) in intermoult
Provedor de dados: Biocell
Autores: Sousa,Liliana G.; Cuartas,Elena I.; Petriella,Ana María.
The aim of this study is to describe the ultrastructure of the hepatopancreas of P. argentinus in intermoult. P. argentinus hepatopancreas was studied using standard TEM techniques. Each tubule consists of four cellular types: E (embryonic), F (fibrillar), R (resorptive) and B (blister like). E-cells have embryonic features and some of them were found in mitosis. F, R and B cells possess an apical brush border. F-cells have a central or basal nucleus, a conspicuous RER, and dilated Golgi cisternae. R cells show a polar organization of organelles in three areas: apical, with numerous mitochondria and sER tubules, a central area with the nucleus and RER, and a basal area containing a sER-like tubule system and mitochondria. B-cells were observed at different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crustacea; Caridea; Hepatopancreas; Ultrastructure.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000100004