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Inflammatory response, parasite load and AgNOR expression in ear skin of symptomatic and asymptomatic Leishmania (Leishmania) chagasi infected dogs
Provedor de dados: 80
Autores: BLA,Verçosa; ACJ,Borges; FJM,Mendes; MLS,Costa; NB,Pereira; MN,Melo; IL,Mendonça; FAL,Costa; AC,Vasconcelos.
The skin has an important role in the transmission of visceral leishmaniasis (VL) as the infection pathway in dogs. To better characterize the inflammatory response of intact skin in VL, sixty infected dogs (30 symptomatic and 30 asymptomatic) and six non-infected controls were studied. Diagnosis of visceral leishmaniasis was confirmed by RIFI and ELISA; direct visualization of the parasite in bone marrow aspirate; imprints of popliteal lymph nodes, spleen, liver and skin; culture in NNN-phase liquid Schneider's medium; and PCR (performed only in the ear skin). Amastigote forms of the parasite in intact skin were found only in symptomatic dogs. Inflammatory infiltrates were observed in all groups, varying from intense and/or moderate in symptomatic to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Inflammation; Parasites; Leishmania chagasi.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300011

Cardiorespiratory evaluation of juvenile rats experimentally envenomed with Tityus serrulatus venom
Provedor de dados: 80
Autores: Pinto,MCL; Melo,MB; Cruz,ML; Verçosa Junior,D; Melo,MM.
Accidental envenomation caused by Tityus serrulatus scorpions is very common in Brazil and may result in serious cardiorespiratory alterations that are frequently fatal to children. In the present study, the effects of T. serrulatus venom on the cardiorespiratory system of recently weaned male Wistar rats were evaluated. Fifteen animals were distributed into three groups (n = 5). The control group A received 400 μL ultrapure water by subcutaneous injection, while the experimental groups B and C were injected with scorpion venom (100 and 450 μg, respectively, in 400 μL water). Electrocardiogram (ECG) traces were obtained prior to the experiment, at five-minute intervals up to 30 minutes after treatment. At 40 minutes after envenomation, the animals had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tityus serrulatus; Rats; Electrocardiography; CK; CK-MB; Troponin; Pulmonary hemorrhages.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200008

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA): molecular background, virulence, and relevance for public health
Provedor de dados: 80
Autores: Bonesso,MF; Marques,SA; Cunha,MLRS.
Staphylococcus aureus and coagulase-negative Staphylococcus (CoNS) are frequently found in nosocomial environments as the main pathogen in several infections. In 1961, reports of nosocomial S. aureus resistant to methicillin, the drug of choice against penicillin-resistant strains, required new alternatives and vancomycin started being used to treat infections caused by methicillin-resistant S. aureus (MRSA). Community-acquired methicillin-resistant S. aureus (CA-MRSA) was first reported in 1990 affecting patients without risk factors for infection with MRSA of hospital origin. MRSA of community origin harbor the genes responsible for the synthesis of Panton-Valentine leukocidin (PVL), a toxin associated with skin and soft tissue infections and that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methicillin-resistant Staphylococcus aureus; MecA gene; Oxacillin; Panton-Valentine leukocidin; Drug resistance; Epidemiology.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400004

Snake venom galactoside-binding lectins: a structural and functional overview
Provedor de dados: 80
Autores: Sartim,Marco A.; Sampaio,Suely V.
AbstractSnake venom galactoside-binding lectins (SVgalLs) comprise a class of toxins capable of recognizing and interacting with terminal galactoside residues of glycans. In the past 35 years, since the first report on the purification of thrombolectin from Bothrops atrox snake venom, several SVgalLs from Viperidae and Elapidae snake families have been described, as has progressive improvement in the investigation of structural/functional aspects of these lectins. Moreover, the advances of techniques applied in protein-carbohydrate recognition have provided important approaches in order to screen for possible biological targets. The present review describes the efforts over the past 35 years to elucidate SVgalLs, highlighting their structure and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; C-type lectin; Galactoside-binding protein; Carbohydrate recognition domain; Glycoconjugates; Inflammatory response; Antitumor activity; Bactericidal activity; Mitogenic activity; Platelet aggregation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100213

Identification and partial purification of an anticoagulant factor from the venom of the Iranian snake Agkistrodon halys
Provedor de dados: 80
Autores: Ghorbanpur,M; Zare Mirakabadi,A; Zokaee,F; Zolfagarrian,H.
An anticoagulant factor was purified from the venom of the Iranian snake Agkistrodon halys by gel filtration on Sephadex G-50 and ion-exchange chromatography on DEAE-Sepharose. In the final stage of purification, the percentage recovery of purified anticoagulant factor was found to be 83%. The purified anticoagulant factor revealed a single protein band in SDS-polyacrylamide electrophoresis under reducing conditions and its molecular weight was about 22 kDa. The purified peptide did not show any effect on casein, BApNA or plasma.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Agkistrodon halys; Anticoagulant factor; Chromatography.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100010

Bee venom treatment reduced C-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome
Provedor de dados: 80
Autores: Karimzadeh,L; Nabiuni,M; Sheikholeslami,A; Irian,S.
Polycystic ovarian syndrome (PCOS) is a low grade inflammatory disease characterized by hyperandrogenemia and chronic anovulation. C-reactive protein (CRP), released by adipocytes, plays a key role in PCOS. Apis mellifera honeybee venom (HBV) contains a variety of biologically active components with various pharmaceutical properties. This study was designed to assess the possibility of HBV application as an anti-inflammatory therapeutic agent. To induce PCOS, 1 mg/100 g body weight estradiol valerate (EV) was subcutaneously (SC) injected into eight-week-old rats. After 60 days, 0.5 mg/kg HBV was administered SC for 14 consecutive days, and the results of PCOS treatment were investigated. Rats were then anesthetized with chloroform, and their ovaries and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polycystic ovarian syndrome (PCOS); Honeybee venom (HBV); C-reactive protein (CRP); Estradiol valerate (EV); Corpus luteum; Chemiluminescence immunoassay.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400006

Pharmacological characterization of venoms from three theraphosid spiders: Poecilotheria regalis, Ceratogyrus darlingi and Brachypelma epicureanum
Provedor de dados: 80
Autores: García-Arredondo,Alejandro; Rodríguez-Rios,Luis; Díaz-Peña,Luis Fernando; Vega-Ángeles,Ricardo.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: &lt; Italic&gt; Poecilotheria regalis&lt; /italic&gt; &lt; Italic&gt; Ceratogyrus darlingi&lt; /italic&gt; &lt; Italic&gt; Brachypelma epicureanum&lt; /italic&gt; Tarantula Venom Toxicity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100323

Although surgery should not be used as first-line treatment, immediate ablation should be performed when necrotic change around the fang mark is recognized
Provedor de dados: 80
Autores: Fujioka,M.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300003

Brazilian journals in electronic format: from diskettes to Open Access
Provedor de dados: 80
Autores: Castro,Regina Célia Figueiredo.
Tipo: Info:eu-repo/semantics/other
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100001

Ergosterol isolated from the basidiomycete Pleurotus salmoneostramineus affects Trypanosoma cruzi plasma membrane and mitochondria
Provedor de dados: 80
Autores: Alexandre,Tatiana Rodrigues; Lima,Marta Lopes; Galuppo,Mariana Kolos; Mesquita,Juliana Tonini; Nascimento,Matilia Ana do; Santos,Augusto Leonardo dos; Sartorelli,Patricia; Pimenta,Daniel Carvalho; Tempone,Andre Gustavo.
Abstract  Background  Major drawbacks of the available treatment against Chagas disease (American trypanosomiasis) include its toxicity and therapeutic inefficiency in the chronic phase of the infection, which makes it a concern among neglected diseases. Therefore, the discovery of alternative drugs for treating chronic Chagas disease requires immediate action. In this work, we evaluated the mushroom Pleurotus salmoneostramineus in the search for potential antiparasitic compounds.  Methods  Fruit bodies of the basidiomycete Pleurotus salmoneostramineus were triturated and submitted to organic solvent extraction. After liquid-liquid partition of the crude extract, three fractions were obtained and the bioguided fractionation study was conducted to isolate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pleurotus salmoneostramineus; Ergosterol; Trypanosoma cruzi; Mechanism of action.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100313

Incidence and severity of scorpion stings in Algeria
Provedor de dados: 80
Autores: Laïd,Y; Boutekdjiret,L; Oudjehane,R; Laraba-Djebari,F; Hellal,H; Guerinik,M; Griene,L; Alamir,B; Merad,R; Chippaux,JP.
Scorpion stings are a public health problem in the Maghreb region. In Algeria, epidemiological data were collected over the past twenty years by the Algerian health authorities. This study is an analysis of morbidity and mortality data collected from 2001 to 2010. Annual incidence and mortality due to scorpion envenoming were 152 ± 3.6 stings and 0.236 ± 0.041 deaths per 100,000 people (95% CI), respectively. The risk of being stung by a scorpion was dramatically higher in southern areas and central highlands due to environmental conditions. Incidence of envenoming was especially higher in the adult population, and among young males. In contrast, mortality was significantly higher among children under 15 years, particularly ages 1-4. Upper limbs were more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion; Stings; Envenoming; Deaths; Epidemiology; Management; Algeria.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400008

Preliminary molecular characterization of a proinflammatory and nociceptive molecule from the Echinometra lucunter spines extracts
Provedor de dados: 80
Autores: Sciani,Juliana Mozer; Zychar,Bianca; Gonçalves,Luis Roberto; Giorgi,Renata; Nogueira,Thiago; Pimenta,Daniel Carvalho.
Abstract  Background  Sea urchins are animals commonly found on the Brazilian shoreline, being Echinometra lucunter the most abundant species. Accidents caused by E. lucunter have been reported as one of the most frequent in Brazil, and are characterized by intense pain and inflammation, consequence of spine puncture in the skin. In order to characterize such toxic effects, we isolated one molecule that caused inflammatory and nociceptive effects.  Methods E. lucunter specimens were collected without gender distinction. Spines were removed and molecules were extracted, fractionated by RP-HPLC and assayed for inflammatory and nociceptive activity, in a biological-driven fractionation way, until the obtainment of one active molecule and its subsequent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxins; Sea urchin; Echinometra lucunter; Spines; Inflammation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100318

Antileishmanial activity and immunomodulatory effect of secosubamolide, a butanolide isolated from Nectandra oppositifolia (Lauraceae)
Provedor de dados: 80
Autores: Costa-Silva,Thais A. da; Conserva,Geanne A. Alves; Galisteo Jr,Andrés J.; Tempone,Andre G.; Lago,João Henrique G.
Abstract  Background: Visceral leishmaniasis is a complex neglected tropical disease caused by Leishmania donovani complex. Its current treatment reveals strong limitations, especially high toxicity. In this context, natural products are important sources of new drug alternatives for VL therapy. Therefore, the antileishmanial and immunomodulatory activity of compounds isolated from Nectandra oppositifolia (Lauraceae) was investigated herein.  Methods: The n-hexane extract from twigs of N. oppositifolia were subjected to HPLC/HRESIMS and bioactivity-guided fractionation to afford compounds 1 and 2 which were evaluated in vitro against Leishmania (L.) infantum chagasi and NCTC cells.  Results: The n-hexane extract displayed activity against L. (L.) infantum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania (L.) infantum chagasi; Antileishmanial; Nectandra oppositifolia; Secosubamolide; Immunomodulatory.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100317

Sandflies (Diptera, Psychodidae) from forest areas in Botucatu municipality, central western São Paulo State, Brazil
Provedor de dados: 80
Autores: Cutolo,André Antonio; Galati,Eunice Aparecida Bianchi; Von Zuben,Claudio José.
Background The study of the distribution and ecology of sandfly species is essential for epidemiological surveillance and estimation of the transmission risk of Leishmania spp. infection. Findings In the present study, sandflies were captured in native fragmented forest areas in Rubião Júnior district, Botucatu municipality, São Paulo state, Brazil, between September 2001 and January 2005. A minimum of two automatic light traps were installed per night from 6 pm to 8 am, in different months, resulting in approximately 900 collecting hours. During this period, 216 sandfly specimens of sixteen species were captured. Pintomyia monticola and Brumptomyia guimaraesi were the most abundant with 56 specimens (25.93%) captured per species, followed by Pintomyia...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cutaneous leishmaniasis; Vector; Sandfly; Brazilian savannah; Semideciduous forest; Cuesta.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100403

Effects of copaiba oil on dermonecrosis induced by Loxosceles intermedia venom
Provedor de dados: 80
Autores: Ribeiro,Mara Fernandes; Oliveira,Felipe Leite de; Souza,Aline Moreira; Machado,Thelma de Barros; Cardoso,Priscilla Farinhas; Patti Sobrinho,Andrea; Nascimento,Angélica Silveira; Souza,Cláudio Maurício Vieira de; Elias,Sabrina Calil.
ABSTRACT  Background: Accidents caused by spiders of the genus Loxosceles constitute an important public health problem in Brazil. The venom of Loxosceles sp induces dermonecrosis at the bite site and systemic disease in severe cases. Traditional medicine based on plant-derived products has been proven to reduce the local effects of envenomation. The present study verified the healing effects of copaiba oil on lesions induced by the venom of L. intermedia.  Methods: Cutaneous lesions were induced on the backs of rabbits by intradermal injection of L. intermedia venom. Copaiba oil was applied topically 6 hours after injection; the treatment was repeated for 30 days, after which animal skins were removed and processed for histopathological analysis. Blood...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Loxosceles intermedia; Venom; Skin lesion; Copaiba oil; Topical treatment.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100310

About a case of blindness following scorpion envenomation
Provedor de dados: 80
Autores: Delma,K.
The author reports a case of blindness occurred after three scorpion stings in a young woman from the region Ouargla, Algeria. The absence of signs of neurological and cardiovascular envenomation and the functional examinations of eyes is likely to be the consequence of a toxic neuropathy. Two months later, blindness persists and functional prognosis remains reserved.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Scorpion sting; Blindness; Toxic neuropathy.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400020

Cytotoxic and apoptotic effects of scorpion Leiurus quinquestriatus venom on 293T and C2C12 eukaryotic cell lines
Provedor de dados: 80
Autores: Omran,M. A. A.
Scorpion venom toxicity is of major concern due to its influence on human activities and public health. The cytotoxicity and apoptosis induced by scorpion L. quinquestriatus venom on two established eukaryotic cell lines (293T and C2C12) were analyzed. Both cultured cell lines were incubated with varying doses (10, 20, and 50 µg/ml) of scorpion venom in serum free medium (SFM) for 0.5, 1, 2, 4, and 8 hours at 37°C. The percentage of total lactate dehydrogenase (LDH) released in the culture during venom incubation was used as an index of cell damage. Control culture was treated with an equal amount of SFM. Cell injury was recognized morphologically and apoptosis was researched by a Fluorescing Apoptosis Detection System using the principle of TUNEL...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venom; Leiurus quinquestriatus; Cytotoxicity; Apoptosis; LDH.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200008

Sequence analysis of the cDNA encoding for SpCTx: a lethal factor from scorpionfish venom ( Scorpaena plumieri )
Provedor de dados: 80
Autores: Costa,Fábio L. S.; Lima,Maria Elena De; Figueiredo,Suely G.; Ferreira,Rafaela S.; Prates,Núbia S.; Sakamoto,Tetsu; Salas,Carlos E.
Abstract  Background: Lethal factors are multifunctional oligomeric proteins found in the venomous apparatus of Scorpaeniformes fish. These toxins elicit not only an array of biological responses in vitro but also cardiovascular disorders and strong hemolytic, nociceptive and edematogenic activities in vivo. This work describes the cloning and molecular identification of two toxin subunits, denominated Sp-CTx-α and Sp-CTx-β, from scorpionfish venom ( Scorpaena plumieri ).  Methods: The primary structures were deduced after cDNA amplification by PCR with primers from conserved sequences described in Scorpaeniformes toxins. Following DNA sequencing and bioinformatic analysis, the tridimensional structures of both subunits were modeled.  Results: The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CDNA; Lethal factor; Scorpaena plumieri; Scorpionfish; Venom gland.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100315

Biotin-avidin sandwich elisa with specific human isotypes IgG1 and IgG4 for Culicidae mosquito blood meal identification from an epizootic yellow fever area in Brazil
Provedor de dados: 80
Autores: Marassá,AM; Paula,MB; Gomes,AC; Consales,CA.
With a view toward investigating the feeding behavior of Culicidae mosquitoes from an area of epizootic yellow fever transmission in the municipalities of Garruchos and Santo Antônio das Missões, Rio Grande do Sul State, Brazil, specimens were collected by aspiration from September 2005 to April 2007. The engorged females were submitted to blood meal identification by enzyme-linked immunosorbent assay (ELISA). A total of 142 blood-engorged samples were examined for human or monkey blood through species-specific IgG. Additional tests for specificity utilizing isotypes IgG1 and IgG4 of human monoclonal antibodies showed that only anti-human IgG1 was effective in recognizing blood meals of human origin. The results indicated a significant difference (p =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Culicidae; ELISA; Feeding habits; Isotypes; Yellow fever.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400008

Analysis of Paracoccidioides brasiliensis exoantigens stability
Provedor de dados: 80
Autores: Silva,Décio Fragata da.
Tipo: Info:eu-repo/semantics/other
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400012

In vitroscreening and evaluation of antivenom phytochemicals from Azima tetracanthaLam. leaves against Bungarus caeruleusand Vipera russelli
Provedor de dados: 80
Autores: Janardhan,Bhavya; Shrikanth,Vineetha M; Mirajkar,Kiran K; More,Sunil S.
Background : Snakebites are considered a neglected tropical disease that affects thousands of people worldwide. Although antivenom is the only treatment available, it is associated with several side effects. As an alternative, plants have been extensively studied in order to obtain an alternative treatment. In folk medicine, Azima tetracanthaLam. is usually used to treat snakebites. The present study aims to provide a scientific explanation for the use of this plant against snakebite. The extracts of shade dried leaves of A. tetracanthawere tested for in vitroinhibitory activity on toxic venom enzymes like phosphomonoesterase, phosphodiesterase, acetylcholinesterase, hyaluronidase etc. from Bungarus caeruleusandVipera russellivenoms.Results : The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetylcholinesterase; Azima tetracantha; Antivenom; Krait; Viper; In vitro.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200318

Purification, characterization, crystallization and theoretical molecular modeling of gyroxin fraction from Crotalus durissus terrificus venom (Laurenti, 1768)
Provedor de dados: 80
Autores: Buchi,A. T.
Tipo: Info:eu-repo/semantics/review
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200020

Technetium-99m-labeled deoxynivalenol from Fusarium mycotoxin alters organ toxicity in BALB/c mice by oral and intravenous route
Provedor de dados: 80
Autores: Chattopadhyay,P; Pandey,A; Goyary,D; Chaurasia,A; Singh,L; Veer,V.
The toxicity of deoxynivalenol, both intravenously and orally, was investigated in male and female BALB/c mice. Technetium-99m (99m Tc)-labeled deoxynivalenol was administered to mice by tail vein injection and orally dosed. Distribution of labeled deoxynivalenol at 26 hours was monitored by gamma-scintigraphy. In the evaluated organs, the accumulation of radioactive deoxynivalenol was correlated with the amount of radioactivity. In addition, the toxicity of deoxynivalenol was measured by biochemical assays followed by histopathological findings. Kidney and hepatic marker enzymes were significantly increased in intravenously administered deoxynivalenol as compared to orally treated mice. Intravenously treated mice showed severe damage in liver and kidney...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Technetium-99m (99mTc); Deoxynivalenol; Biodistribution; Toxicity; Histopathology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300002

Insecticidal activity of Leptodactylus knudseni andPhyllomedusa vaillantii crude skin secretions against the mosquitoes Anopheles darlingi andAedes aegypti
Provedor de dados: 80
Autores: Trindade,Frances TT; Soares,Ângela A; Moura,Andréa A de; Rego,Tiago B; Soares,Andreimar M; Stábeli,Rodrigo G; Calderon,Leonardo A; Almeida e Silva,Alexandre de.
Background Mosquitoes are important vectors of several diseases, including malaria and dengue, and control measures are mostly performed using chemical insecticides. Unfortunately, mosquito resistance to commonly applied insecticides is widespread. Therefore, a prospection for new molecules with insecticidal activity based on Amazon biodiversity using the anuransLeptodactylus knudseni andPhyllomedusa vaillantii was performed against the mosquito speciesAnopheles darlingi and Aedes aegypti.Methods The granular secretion from anuran skin was obtained by manual stimulation, and lethal concentrations (LCs) for larvicidal and adulticidal tests were calculated using concentrations from 1-100 ppm. The skin secretions from the anuran species tested caused...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vector control; Anuran amphibians; Dengue; Malaria.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200334

The unfulfilled promises of scorpion insectotoxins
Provedor de dados: 80
Autores: Ortiz,Ernesto; Possani,Lourival D.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinsecticides; Insectotoxins; Scorpion venom.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100205

Serological survey for rabies in serum samples from vampire bats (Desmodus rotundus) in Botucatu region, SP, Brazil
Provedor de dados: 80
Autores: Langoni,H; Souza,LC; Zetun,CB; Silva,TCC; Hoffmann,JL; Silva,RC.
The chiropterans constitute 25% of the world's mammal fauna. Due to the destruction of their natural ecosystem, the vampire bats have moved from nature to artificial roosts closer to man and domestic animals. This phenomenon has happened particularly in rural areas. Rabies is a viral anthropozoonosis, 100% lethal, and vampire bats (Desmodus rotundus) represent an important role in its epidemiology. D. rotundus were captured at night with mesh nets in partnership with the Botucatu Defense Office and sent to the Zoonosis Diagnostic Service, at the School of Veterinary Medicine and Animal Husbandry, UNESP. Serum samples from 204 bats were analyzed by enzyme-linked immunosorbent assay (ELISA) and fluorescent antibody viral neutralization test (FAVN) for rabies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Desmodus rotundus; Vampire bats; Rabies virus; LPC-ELISA; RFFIT.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400008

Fibrin biopolymer as scaffold candidate to treat bone defects in rats
Provedor de dados: 80
Autores: Cassaro,Claudia Vilalva; Justulin Jr.,Luis Antonio; Lima,Patrícia Rodrigues de; Golim,Marjorie de Assis; Biscola,Natália Perussi; Castro,Mateus Vidigal de; Oliveira,Alexandre Leite Rodrigues de; Doiche,Danuta Pulz; Pereira,Elenize Jamas; Ferreira Jr.,Rui Seabra; Barraviera,Benedito.
ABSTRACT  Background:  Bone tissue repair remains a challenge in tissue engineering. Currently, new materials are being applied and often integrated with live cells and biological scaffolds. The fibrin biopolymer (FBP) proposed in this study has hemostatic, sealant, adhesive, scaffolding and drug-delivery properties. The regenerative potential of an association of FBP, biphasic calcium phosphate (BCP) and mesenchymal stem cells (MSCs) was evaluated in defects of rat femurs.  Methods:  Adult male Wistar rats were submitted to a 5-mm defect in the femur. This was filled with the following materials and/or associations: BPC; FBP and BCP; FBP and MSCs; and BCP, FBP and MSCs. Bone defect without filling was defined as the control group. Thirty and sixty days...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone regeneration; Biomaterials; Fibrin sealant; Fibrin biopolymer; Biphasic calcium phosphate; Mesenchymal stem cells.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100320

Maintenance of scorpions of the genus Tityus Koch (Scorpiones, Buthidae) for venom obtention at Instituto Butantan, São Paulo, Brazil
Provedor de dados: 80
Autores: Candido,D. M.; Lucas,S.
This work presents the experience of the Laboratory of Arthropods at Instituto Butantan, which maintains scorpions in captivity in order to obtain the venom used in the production of anti-arachnid serum. Between 1993 and 2000, the laboratory received 24.781 specimens of Tityus serrulatus in order to obtain poison for the production of anti-scorpion serum. In the first extraction, performed by electrical stimulation, the animals gave an average quantity of 0.4 mg venom per specimen. Animal acquisition and involved professional safety are considered. In addition, the captivity, handling and feeding techniques are described, as well as the method and equipment used for venom extraction. It shows the importance of regular campaigns, offering information to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpions; Tityus; Maintenance; Venom.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000100007

Cytotoxicity of Southeast Asian snake venoms
Provedor de dados: 80
Autores: Jamunaa,A; Vejayan,J; Halijah,I; Sharifah,SH; Ambu,S.
Cytotoxicity of venoms from eleven medically important snakes found in Southeast Asia (Naja kaouthia, Naja siamensis, Naja sumatrana, Ophiophagus hannah, Bungarus candidus, Bungarus fasciatus, Enhydrina schistosa, Calloselasma rhodostoma, Trimeresurus purpureomaculatus and Tropidolaemus sumatranus) was determined, based on the MTS cytotoxicity assay, which determines the survival of viable cells in monolayer MDCK and Vero cell cultures upon exposure to the snake venoms. Snake venom toxicity was expressed as the venom dose that killed 50% of the cells (CTC50) under the assay conditions. Venoms of C. rhodostoma (2.6 µg/mL, 1.4 µg/mL) and O. hannah were the most cytotoxic (3.8 µg/mL, 1.7 µg/mL) whereas N. siamensis venom showed the least cytotoxicity (51.9...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Snake venom; Vipers; Elapids; Kraits.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200004

Unique heterologous fibrin biopolymer with hemostatic, adhesive, sealant, scaffold and drug delivery properties: a systematic review
Provedor de dados: 80
Autores: Buchaim,Daniela Vieira; Cassaro,Claudia Vilalva; Shindo,João Vitor Tadashi Cosin; Coletta,Bruna Botteon Della; Pomini,Karina Torres; Rosso,Marcelie Priscila de Oliveira; Campos,Leila Maria Guissoni; Ferreira Jr,Rui Seabra; Barraviera,Benedito; Buchaim,Rogério Leone.
Abstract Fibrin biopolymers, previously referred as “fibrin glue” or “fibrin sealants”, are natural biomaterials with diverse applications on health. They have hemostatic, adhesive, sealant, scaffold and drug delivery properties and have become widely used in medical and dental procedures. Historically, these biomaterials are produced from human fibrinogen and human or animal thrombin, and the possibility of transmission of infectious diseases by human blood is not ruled out. In the 1990s, to overcome this problem, a new heterologous biomaterial composed of a thrombin-like enzyme purified from Crotalus durissus terrificus venom and a cryoprecipitate rich in fibrinogen extracted from buffaloes Bubalus bubalis blood has been proposed. Therefore, a systematic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibrin sealant; Snake venom; Thrombin-like enzyme; Cryoprecipitate coagulum.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100207

Synthesis and antimicrobial evaluation of two peptide LyeTx I derivatives modified with the chelating agent HYNIC for radiolabeling with technetium-99m
Provedor de dados: 80
Autores: Fuscaldi,Leonardo Lima; Santos,Daniel Moreira dos; Pinheiro,Natália Gabriela Silva; Araújo,Raquel Silva; Barros,André Luís Branco de; Resende,Jarbas Magalhães; Fernandes,Simone Odília Antunes; Lima,Maria Elena de; Cardoso,Valbert Nascimento.
Abstract  Background  Current diagnostic methods and imaging techniques are not able to differentiate septic and aseptic inflammation. Thus, reliable methods are sought to provide this distinction and scintigraphic imaging is an interesting option, since it is based on physiological changes. In this context, radiolabeled antimicrobial peptides have been investigated as they accumulate in infectious sites instead of aseptic inflammation. The peptide LyeTx I, from the venom of Lycosa erythrognatha, has potent antimicrobial activity. Therefore, this study aimed to synthesize LyeTx I derivatives with the chelating compound HYNIC, to evaluate their antimicrobial activity and to radiolabel them with 99mTc.  Methods  Two LyeTx I derivatives, HYNIC-LyeTx I...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Septic and aseptic inflammation; Differential diagnosis; Antimicrobial peptides LyeTx I derivatives; MALDI-ToF-MS RP-HPLC; Technetium-99m; HYNIC EDDA Tricine.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100312

Arthropod venom Hyaluronidases: biochemical properties and potential applications in medicine and biotechnology
Provedor de dados: 80
Autores: Bordon,Karla C F; Wiezel,Gisele A.; Amorim,Fernanda G.; Arantes,Eliane C.
AbstractHyaluronidases are enzymes that mainly degrade hyaluronan, the major glycosaminoglycan of the interstitial matrix. They are involved in several pathological and physiological activities including fertilization, wound healing, embryogenesis, angiogenesis, diffusion of toxins and drugs, metastasis, pneumonia, sepsis, bacteremia, meningitis, inflammation and allergy, among others. Hyaluronidases are widely distributed in nature and the enzymes from mammalian spermatozoa, lysosomes and animal venoms belong to the subclass EC 3.2.1.35. To date, only five three-dimensional structures for arthropod venom hyaluronidases (Apis mellifera and Vespula vulgaris) were determined. Additionally, there are four molecular models for hyaluronidases fromMesobuthus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyaluronidase; Scorpion; Spider; Caterpillar; Hymenoptera; Insects; Cloning; Heterologous expression; PEGylation; Biotechnological applications.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100214

Role of TLR2 and TLR4 on human neutrophil functions against Paracoccidioides brasiliensis
Provedor de dados: 80
Autores: Acorci-Valério,M. J.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300020

Occurrence and risk factors associated with canine leptospirosis
Provedor de dados: 80
Autores: Kikuti,M; Langoni,H; Nobrega,DN; Corrêa,APFL; Ullmann,LS.
Leptospirosis is a globally distributed emerging zoonosis. Dogs are commonly affected and although other serovars can cause canine leptospirosis, Leptospira interrogans serovar Canicola is primary found in these animals. A retrospective study was conducted using a database of 1195 dogs tested for Leptospira infection from 2003 to 2010 at the Laboratory of Zoonosis Diagnosis at the Veterinary Hospital of São Paulo State University (UNESP) in Botucatu, São Paulo state, Brazil. The seroprevalence of infected dogs was 20.08% (240/1195), and the most prevalent serovars were Canicola (6.7%), Copenhageni (5.0%), Icterohaemorrhagiae (2.9%), Autumnalis (2.9%), Pyrogenes (2.8%), Pomona (2.0%), Hardjo (2.0%), Australis (1.8%), Bratislava (1.6%), Cynopteri (1.4%),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira spp.; Dog; Serology; Epidemiology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100016

On some toxinological aspects of the starfish Stellaster equestris (Retzius, 1805)
Provedor de dados: 80
Autores: Kanagarajan,U; Bragadeeswaran,S; Venkateshvaran,K.
Whole-body extracts in methanol were obtained from the starfish Stellaster equestris. The crude toxin was fractionated stepwise using diethylaminoethyl (DEAE) cellulose column chromatography. The crude toxin was lethal to male albino mice at a dose of 1.00 mL (containing 531.0 µg/mL protein) when injected intraperitoneally (IP) but the toxicity was abolished in all cases except one upon fractionation. The crude toxin and all the adsorbed fractions exhibited potent hemolytic activity on chicken, goat and human blood. However, group B human erythrocytes were resistant to lysis by all fractions and group O by most of the fractions. Paw edema in mice was caused by the crude toxin and all fractions. Pheniramine maleate and piroxicam blocked the toxicity when...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Starfish; Toxicity; Hemolysis; Human blood groups; Paw edema.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300005

On frogs, toxins and true friendship: an atypical case report
Provedor de dados: 80
Autores: Daniel-Ribeiro,Cláudio Tadeu; Roussilhon,Christian.
Abstract The authors report a series of events including the scientific interest for poisonous dendrobates of French Guiana, the human confrontation with the immensity of the evergreen rainforest, the fragility of the best-prepared individuals to a rough life, and the unique and very special manifestation of a solid friendship between two experts and enthusiasts of outdoor life. In the evergreen forest of South America, as in many other scientific field expeditions, everything may suddenly go wrong, and nothing can prepare researchers to accidents that may occur in a succession of uncontrollable errors once the first mistake is done. This is what happened during an expedition in search for dendrobates by an experienced forest guide and naturalist. The...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Dart frogs; Dendrobatidae; Toxins; French Guiana.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100501

Euscorpius (Scorpiones, Euscorpiidae): three cases of stings in northwestern Italy
Provedor de dados: 80
Autores: Dutto,M; Dutto,L; Scaglione,N; Bertero,M.
In the period between June 2008 and August 2009, three cases of stings of Euscorpius scorpions indigenous to Italy were treated at two different emergency departments (ED) in hospitals of the Piedmont region, northwest Italy: Santa Croce e Carle General Hospital in Cuneo, and Santissima Annunziata Hospital in Savigliano. Scorpion stings in Italy are rare and not well documented in the literature; this situation may raise doubts among medical personnel as to how such lesions are best treated. Analysis of the incidents confirms that the venom of Euscorpius do not provokes systemic poisoning in humans and in these cases even dermatological reactions were not significant.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Euscorpius; Scorpion; Sting; Scorpionism; Venom; Poisoning; Italy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400018

Feeding habits of mosquitoes (Diptera: Culicidae) in an area of sylvatic transmission of yellow fever in the state of São Paulo, Brazil
Provedor de dados: 80
Autores: Mucci,Luis Filipe; Cardoso Júnior,Rubens Pinto; Paula,Marcia Bicudo de; Scandar,Sirle Abdo Salloum; Pacchioni,Márcio Lunardeli; Fernandes,Aristides; Consales,Cleide Aschenbrenner.
AbstractBackground The reintroduction of sylvatic yellow fever in the state of São Paulo after about six decades was confirmed in the Northwestern region in 2000, where in 2008 there also occurred an important epizootic. The purpose of this study was to investigate the feeding habits of culicids potentially involved in the sylvatic transmission of the virus in this region.Methods Specimens were collected in 24 forested localities at ground level with hand nets and mouth aspirators. Collections were made quarterly between October 2006 and July 2008 during daylight hours. Blood-meal identification was carried out in mosquitoes of the tribes Aedini, Mansoniini and Sabethini. The biotin/avidin sandwich ELISA was employed to determine six source types: bird,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feeding habit; Sylvatic yellow fever; Neotropical mosquitoes; Non-human primate; Forest fragmentation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100316

In vivo pharmacological study on the effectiveness of available polyclonal antivenom against Hemiscorpius lepturus venom
Provedor de dados: 80
Autores: Jalali,A; Pipelzadeh,M H; Seyedian,R; Rahmani,A H; Omidian,N.
The available Razi Institute antivenom is still, empirically, used by intramuscular (IM) administration for the treatment of scorpion stings in humans by six medically dangerous species including Hemiscorpius lepturus (H. lepturus). The aim of this study was to assess the neutralizing ability and effectiveness of the antivenom in inhibiting hemoglobinuria, biochemical changes, increased microalbuminuria and urinary lactate dehydrogenase (LDH) following H. lepturus sting. Simultaneous intramuscular administration of 10 μL and 100 μL of antivenom, after 24 hours, had no significant preventive effect on the extent and degree of hemoglobinuria or proteinuria produced in venom-treated rats. After IM administration of antivenom, no significant changes in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemiscorpius lepturus; Scorpion; Venom; Hemolysis; Antivenom.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200004

Detection and neutralization of venom by ovine antiserum in experimental envenoming by Bothrops jararaca
Provedor de dados: 80
Autores: Peres,C. M.; Bastos,M. F.; Ferreira,J.; Sartori,A.
In this study we optimized an enzyme-linked immunosorbent assay (ELISA) to evaluate bothropic venom levels in biological samples. These samples were obtained by two distinct protocols. In the first one, Swiss mice were injected with 1 LD50 of Bothrops jararaca (B. jararaca) venom and 15 minutes later, animals were treated with ovine antibothropic serum. Blood and spleen homogenate samples were obtained 6 hours after antiserum therapy. Ovine antibothropic serum significantly neutralized venom levels in serum and spleen. In the second protocol, BALB/c mice were injected with 1 LD50 of bothropic venom by either intraperitoneal (IP) or intradermal (ID) route and venom levels were evaluated 1, 3 and 6 hours after, in blood, spleen homogenates and urine. Serum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararaca; Enzyme-linked immunosorbent assay; Ovine anti-bothropic serum; Venom neutralization.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100010

Evaluation of effects of photooxidized Vespa orientalis venom on memory and learning in rats
Provedor de dados: 80
Autores: Mukund,H; Gawade,SP.
Wasp venom is mixture of complex proteins that have several physical and pharmacological properties. The photochemical detoxification of Vespa orientalis venom is expected to generate photooxidized venom sac extract (PVSE). Antigenically active PVSE is obtained by exposing the venom sac extract (VSE) of Vespa orientalis to ultraviolet radiation in the presence of methylene blue. The aim of the present work was to evaluate the effect of PVSE on learning and memory of rats. Detoxification of PVSE was evident since treated mice had longer survival time than the group of mice treated with VSE. Photooxidized VSE of V. orientalis revealed enhancement on learning and memory by shortening the time to reach food (TRF) in T-maze. In a 28-day study with rats, we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vespa venom; Photochemical processes; Learning; Memory; Maze learning.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400009

Occurrence of heavy metals and antibiotic resistance in bacteria from internal organs of american bullfrog (Rana catesbeiana) raised in Malaysia
Provedor de dados: 80
Autores: Lee,SW; Najiah,M; Wendy,W; Nadirah,M; Faizah,SH.
A total of 40 bacteria have been successfully isolated from internal organs of the American bullfrog (Rana catesbeiana) raised in Malaysia, namely, eight isolates of Aeromonas spp., 21 of Edwardsiella spp., six of Flavobacterium spp. and five of Vibrio spp. In terms of antibiotic susceptibility testing, each isolate was tested against 21 antibiotics, resulting in 482 (57.3%) cases of sensitivity and 61 (7.3%) cases of partial sensitivity. Meanwhile, 297 (35.4%) bacterial isolates were registered as resistant. The multiple antibiotic resistance (MAR) index of each bacterial species indicated that bacteria from raised bullfrogs have been exposed to tested antibiotics with results ranging from 0.27 to 0.39. Additionally, high percentages of heavy metal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rana catesbeiana; Antibiogram; Heavy metal resistance; Multiple antibiotic resistance; Aeromonas spp.; Edwardsiella spp.; Flavobacterium spp.; Vibrio spp.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200016

Hepatitis C in prisons
Provedor de dados: 80
Autores: Alavian,SM.
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300002

Central retinal artery occlusion: an unusual complication of snakebite
Provedor de dados: 80
Autores: Bhalla,A.; Jain,A. P.; Banait,S.; Jajoo,U. N.; Kalantri,S.P.
Snakebites are endemic in some parts of India, being associated with a number of complications. Ocular disturbances are rare, except for injury to the cornea or conjunctiva when the eye is directly exposed to the venom. In this work, we present a case of central retinal artery occlusion caused by snakebite.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Central retinal artery; Snakebite; Saw-scaled viper; Echis carinatus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300009

Mycobacterium marinum infection: a case report
Provedor de dados: 80
Autores: Sette,Christiane Salgado; Wachholz,Patrick Alexander; Masuda,Paula Yoshiko; Figueira,Renata Borges Fortes da Costa; Mattar,Fernanda Rodrigues de Oliveira; Ura,Deise Godoy.
AbstractThe infection by Mycobacterium marinum in humans is relatively uncommon. When it occurs, it mainly affects the skin, usually with a chronic, indolent and benign evolution. The diagnosis requires a high index of suspicion, and a significant delay may be observed between the first symptoms to the final diagnosis. This present case reports a M. marinum infection in an immunocompetent patient that had a chronic undiagnosed injury on the dominant hand for at least five years. The patient had several medical consultations, without proper suspicion, hampering adequate diagnostic investigation. Histopathology detected tuberculoid granulomas, but showed no acid-fast bacilli. The culture in appropriate medium and the polymerase chain reaction-restriction...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Mycobacterium infections; Mycobacterium marinum; Case report; Granuloma.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100412

Report on the occurrence of Haplometroides buccicola (Trematoda, Digenea, Plagiorchiidae) infecting Phalotris lativittatus (Serpentes, Colubridae) in Brazil
Provedor de dados: 80
Autores: Silva,R. J.; Andrade,P. A.; Monteiro e Silva,H. A.; Rossellini,M.; Barrella,T. H.
Haplometroides buccicola (Trematoda, Digenea, Plagiorchiidae) was reported in the mouth and oesophagus of Phalotris lativittatus (Serpentes, Colubridae) from Botucatu, São Paulo State, Brazil. This is the first report on the occurrence of H. buccicola parasitizing P. lativittatus. The Haplometroides genus was also discussed and the most important morphological characters for the identification of the species H. buccicola and H. odhneri are presented.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haplometroides buccicola; Trematoda; Plagiorchiidae; Phalotris lativitattus; Serpentes; Colubridae.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300013

Quality of horse F(ab′)2 antitoxins and antirabies immunoglobulins: protein content and anticomplementary activity
Provedor de dados: 80
Autores: Squaiella-Baptistão,Carla Cristina; Magnoli,Fábio Carlos; Marcelino,José Roberto; Sant’Anna,Osvaldo Augusto; Tambourgi,Denise V.
Abstract  Background Among other applications, immunotherapy is used for the post-exposure treatment and/or prophylaxis of important infectious diseases, such as botulism, diphtheria, tetanus and rabies. The effectiveness of serum therapy is widely proven, but improvements on the immunoglobulin purification process and on the quality control are necessary to reduce the amount of protein aggregates. These may trigger adverse reactions in patients by activating the complement system and inducing the generation of anaphylatoxins. Herein, we used immunochemical methods to predict the quality of horse F(ab′)2 anti-botulinum AB, anti-diphtheric, antitetanic and anti-rabies immunoglobulins, in terms of amount of proteins and protein aggregates.  Methods Samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heterologous immunoglobulin; F(ab′)2 fragment; Antitoxins; Anti-rabies; Protein profile; Complement system.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100309

Deinagkistrodon acutus envenomation: a report of three cases
Provedor de dados: 80
Autores: Cheng,Chin-Lung; Mao,Yan-Chiao; Liu,Po-Yu; Chiang,Liao-Chun; Liao,Shu-Chen; Yang,Chen-Chang.
Abstract  Background Deinagkistrodon acutus envenomation is associated with severe hematological and wound complications but is rarely described.  Case presentation  Herein, we report three cases of victims bitten by D. acutus and indicate that rapid-onset severe coagulopathy and thrombocytopenia are distinct features of D. acutus snakebite, which are not observed in other crotaline snakebites (i.e., Trimeresurus stejnegeri and Protobothrops mucrosquamatus) in Taiwan. The toxic effects could occur as early as 2 to 3 h following D. acutus envenomation and persist if the administration of specific antivenom is delayed or even not commenced. Based on our findings, 2 to 4 vials of specific antivenom as the first dose should be administered to victims and...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Coagulopathy; Thrombocytopenia; Envenomation; Deinagkistrodon acutus; Snakebite.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100504

Hemolitic action of Naja naja atra cardiotoxin on erythrocytes from different animals
Provedor de dados: 80
Autores: Troiano,J. C.; Gould,E. G.; Gould,I.
A comparative study on the sensitivity of erythrocytes from different vertebrate species (avian, mammalian and reptilian) to the hemolytic action caused by cardiotoxin isolated from Naja naja atra venom was carried out. Cardiotoxin was able to induce direct hemolysis in washed erythrocytes from several animals, except for llama. The EC50 values from hemolysis of the most sensitive (cat) and the most resistant (snake) animal varied approximately tenfold. According to the cell behavior, it was possible to characterize four types of behavior: The first was observed in cat, horse and human cells; the second in rat, rabbit and dog erythrocytes; and the third only in llama erythrocytes, which were resistant to cardiotoxin concentrations up to 300 µg/ml. Finally,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemolysis; Cobra; Venom; Erythrocytes; Animals.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100004

Hematology and plasma biochemistry of wild-caught Indian cobra Naja naja(Linnaeus, 1758)
Provedor de dados: 80
Autores: Parida,Siba Prasad; Dutta,Sushil Kumar; Pal,Arttatrana.
Background : Hematology and plasma biochemistry parameters are useful in the assessment and management of snake physiological status. Although reference ranges are readily available for many snake species, they are lacking for most venomous ophidians. We determined hematology and plasma biochemistry reference ranges for the wild-caught Indian cobra, Naja naja.Results : Blood samples, taken from the ventral tail vein, were assessed for erythrocyte count, total leukocyte count, hemoglobin concentration, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration, considering the sex of snakes. Results revealed the erythrocyte numbers (male, 390000 ± 12503.33/mm3 and female, 347500 ± 7505.55/mm3), shapes and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reference range; Snake; Naja naja; Hematology; Plasma biochemistry.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200320

Odynophagia following retained bee stinger
Provedor de dados: 80
Autores: Viswanathan,S; Viswanathan,S; Iqbal,N; Shanmugam,V; Srinath,G.
Nearly half of Hymenoptera stings affect the head and neck region of victims, but reports on oropharyngeal bee stings are very few. We describe the case of a patient with odynophagia and suffocation in mass envenomation. He had a retained bee stinger whose removal was delayed for more than 24 hours following the sting, due to persisting angioedema. Odynophagia receded after removal of the stinger and treatment with paracetamol, steroids and metronidazole. The patient also developed rhabdomyolysis, renal failure and hepatitis that were treated with conservative therapy. Oropharyngeal stings can simulate symptoms of persisting angioedema in victims of mass envenomation.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Intraoral bee sting; Massive bee sting; Oropharyngeal Hymenoptera stings; Soft palate.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200016

Neurotransmitter evaluation in the hippocampus of rats after intracerebral injection of TsTX scorpion toxin
Provedor de dados: 80
Autores: Nencioni,ALA; Barreto,SA; Lebrun,I; Florio,JC; Lourenço,GA; Dorce,VAC.
TsTX is an α-type sodium channel toxin that stimulates the discharge of neurotransmitters from neurons. In the present study we investigated which neurotransmitters are released in the hippocampus after TsTX injection and if they are responsible for electrographic or histopathological effects. Microdialysis revealed that the toxin increased glutamate extracellular levels in the hippocampus; however, levels of gamma-aminobutyric acid (GABA), glycine, 5-hydroxyindoleacetic acid (5-HIAA), homovanillic acid (HVA) and 3,4-dihydroxyphenylacetic acid (DOPAC) were not significantly altered. Neurodegeneration in pyramidal cells of hippocampus and electroencephalographic alterations caused by the toxin were blocked by pretreatment with riluzole, a glutamate release...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion toxin; Microdialysis; Hippocampus; Neurotransmitters; Neurodegeneration; Riluzole.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200007

Experimental infection of Leishmania chagasi in immunosuppressed balb/c mice: cellular immune response and parasite burden
Provedor de dados: 80
Autores: Hoffmann,J. L.
Tipo: Info:eu-repo/semantics/other
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400017

Histology and histochemistry of the venom apparatus of the black scorpion Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae)
Provedor de dados: 80
Autores: Jarrar,BM; Al-Rowaily,MA.
The venom apparatus of the black scorpion Androctonus crassicauda has been characterized histologically and histochemically in the present study. The results showed that this apparatus consists of paired venom glands, each of which initially presents its own canal and posteriorily both fuse into a single common one. Each gland is covered by a sheath of striated muscle and is lined with extensively folded secretory epithelium (formed of non-secretory and secretory venom-producing cells). The outcomes also revealed that the venom-producing cells of both glands produce neutral mucosubstances, sialomucins, sulfomucins and proteins, but are devoid of glycogen. Cysteine, tyrosine, tryptophan and arginine were also detected along with activities of acid and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Androctonus; Scorpion; Venom apparatus; Histochemistry; Phosphatases; Esterases; Peptidases.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300010

Detection of shellfish toxins from scallops in Guangzhou seafood market
Provedor de dados: 80
Autores: Huazhang,L; Wen-xue,L; Quan-xin,Z; Guang-yu,Y; Jun-tao,L; Li,M; Wei,Z.
To evaluate scallop safety in the Guangzhou seafood market, contents of shellfish toxins in adductor muscle, mantle skirts, gills and visceral mass of scallops were examined using enzyme-linked immunosorbent assay (ELISA) and mouse unit assay. The results showed that: paralytic shellfish poisoning contents were up to 37.44 μg/100 g by ELISA and 319.99 MU/100 g by mouse unit assay, which did not exceed the limits of national standards (80 μg/100g and 400 MU/100 g); the contents of diarrhetic shellfish poisoning were 142.04 μg/100g and 0.2 MU/100 g, which exceeded the national standard limits (60 μg/100g); neurotoxic shellfish poisoning was undetectable; the contents of amnesic shellfish poisoning reached 220.12 μg/100g (no limit value could be referred to)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paralytic shellfish poisoning; Diarrheic shellfish poisoning; Neurotoxic shellfish poisoning; Amnesic shellfish poisoning; Enzyme-linked immunosorbent assay; Mouse unit assay.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100010

Characterization of Leiurus abdullahbayrami (Scorpiones: Buthidae) venom: peptide profile, cytotoxicity and antimicrobial activity
Provedor de dados: 80
Autores: Erdeş,Efe; Doğan,Tuğba Somay; Coşar,İlhan; Danışman,Tarık; Kunt,Kadir Boğaç; Şeker,Tamay; Yücel,Meral; Özen,Can.
Background Scorpion venoms are rich bioactive peptide libraries that offer promising molecules that may lead to the discovery and development of new drugs.Leiurus abdullahbayrami produces one of the most potent venoms among Turkish scorpions that provokes severe symptoms in envenomated victims.Methods In the present study, the peptide profile of the venom was investigated by electrophoretic methods, size-exclusion and reversed-phase chromatography and mass spectroscopy. Cytotoxic and antimicrobial effects were evaluated on a breast cancer cell line (MCF-7) and various bacterial and fungal species.Results Proteins make up approximately half of the dry weight of L. abdullahbayrami crude venom. Microfluidic capillary electrophoresis indicated the presence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venom; Toxin; Peptide; Leiurus abdullahbayrami; Microfluidic capillary; Electrophoresis; Peptidomics; Venomics; Cytotoxicity; Antimicrobial activity; Turkey.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200338

Oral manifestations of syphilis: a review
Provedor de dados: 80
Autores: Soares,A.B.; Gonzaga,H.F.S.; Jorge,M.A.; Barraviera,S.R.C.S.
Syphilis is an infectious disease presenting stages associated with specific oral lesions. Therefore, health professionals should be familiar with the different syphilis oral manifestations at each stage and be prepared to refer any suspected patient for further evaluation. This report describes the most important clinical factors of each stage, emphasizing the oral manifestations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Syphilis; Oral lesions; Treponema pallidum.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000100002

rACLF, a recombinant snake venom metalloprotease, activates endothelial cells in vitro
Provedor de dados: 80
Autores: De Moraes,C. K.; Fritzen,M.; Chudzinski-Tavassi,A. M.; Selistre-de-Araújo,H. S.
Snake venom metalloproteases (SVMPs) comprise a family of snake venom toxins responsible for most of local and systemic effects observed during envenomation by snakes from the Viperidae family. The vascular system and more specifically the endothelium seem to be the preferential targets of these proteins. This work describes the effects of rACLF, a recombinant SVMP from Agkistrodon contortrix laticinctus on human umbilical vein endothelial cells (HUVECs) in vitro. Our results showed that rACLF activates HUVECs by the release of mediators involved in inflammation and hemostasis such as prostacyclin and interleukin-8. We also demonstrated that rACLF increased the expression of ICAM-I and decay accelerating factor (DAF). Moreover, rACLF protects the HUVECs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RACLF; Snake venom metalloprotease; Endothelial cells; Inflammation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100009

Antibacterial properties of contact defensive secretions in neotropical Crematogaster ants
Provedor de dados: 80
Autores: Quinet,Y; Vieira,RHSF; Sousa,MR; Evangelista-Barreto,NS; Carvalho,FCT; Guedes,MIF; Alves,CR; de Biseau,JC; Heredia,A.
Crematogaster ants use their contact venoms to compete with other ants. Although those venoms are used primarily as repellent and toxic secretions, they may have other functions. The present study aimed to test the antibacterial property of abdominal venom of three neotropical Crematogaster ant species (C. distans, C. pygmaea and C. rochai) against gram-negative (Escherichia coli and Pseudomonas aeruginosa) and gram-positive (Enterococcus faecalis and Staphylococcus aureus) bacteria. Sterile filter paper was soaked with C. distans, C. pygmaea or C. rochai crude venom and placed on an agar dish that was inoculated with bacterial suspensions. The agar dish was incubated overnight at 37ºC and examined for zones of growth inhibition. For each tested venom and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hymenoptera; Formicidae; Crematogaster; Sting apparatus; Contact venoms.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400013

Morphometric characteristics and seasonal proximity to water of the Cypriot bluntnosed viper Macrovipera lebetina lebetina (Linnaeus, 1758)
Provedor de dados: 80
Autores: Jestrzemski,Daniel; Kuzyakova,Irina.
Abstract  Background: The blunt-nosed viper Macrovipera lebetina (Linnaeus, 1758) is a medically important snake species in the Middle East. Its nominate subspecies Macrovipera l. lebetina is confined to Cyprus, where it is the only dangerously venomous snake species and heavily pursued. Despite the viper's large size, data on its body mass and sex-specific morphological differences are scarce. It is commonly believed that M. l. lebetina prefers freshwater proximity during summer. Hence, we aimed at investigating M. l. lebetina sex-specific morphological differences and its possible attraction to freshwater bodies in late summer.  Methods: Morphometric characteristics, proximity to water and conservation status of M. l. lebetina were investigated in Paphos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyprus; Blunt-nosed viper; Morphology; Body condition index; Ecology; Water; Snakebite; Conservation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100332

Experimental envenomation with Crotalus durissus terrificus venom in dogs treated with antiophidic serum - part II: laboratory aspects, electrocardiogram and histopathology
Provedor de dados: 80
Autores: Nogueira,R. M. B.; Sakate,M.; Sangiorgio,F.; Laposy,C. B.; Tostes,R. A.
The present work shows laboratory aspects, electrocardiogram and histopathology results during experimental envenomation by Crotalus durissus terrificus in dogs treated with antiophidic serum. Twenty-one dogs were divided into three groups of seven animals each. Group I received 1mg/kg venom (sc); Group II received 1mg/kg venom (sc), 50mg antiophidic serum (iv) and fluid therapy including 0.9% NaCl solution (iv); and Group III received 1mg/kg venom (sc), 50mg antiophidic serum (iv) and fluid therapy including 0.9% NaCl solution containing sodium bicarbonate diluted to the dose of 4mEq/kg. Urinalysis showed brown urine, proteinuria, occult blood and myoglobinuria. Respiratory acidosis and hypotension were also observed. At the venom inoculation site, there...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durissus terrificus; Dog; Laboratory aspects; Histopathology.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400011

Management of severe scorpion sting at rural settings: what is the role of scorpion antivenom?
Provedor de dados: 80
Autores: Bawaskar,H. S.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100002

Environmental risk factors for canine toxoplasmosis in a deprived district of Botucatu, SP, Brazil
Provedor de dados: 80
Autores: Camossi,LG; Faccioli,PY; Menozzi,BD; Daher,SR; Langoni,H.
Toxoplasmosis is a worldwide zoonosis caused by Toxoplasma gondii that can infect a large variety of animals, including humans. The present study aimed to evaluate the frequency of anti-T.gondii antibodies in dogs from a peripheral district of Botucatu and to establish the association among some epidemiological variables in order to evaluate risk factors for toxoplasmosis infection. Serum samples from dogs were screened using an indirect fluorescent antibody (IFA) test. Anti-T.gondii antibody prevalence was 56%. The highest titer was 1024 (1.79%) and the most frequent titers were 16 (57.14%) and 64 (33.93%). The chi-square (X²) test revealed significant association among variables such as dog access to street, ingestion of raw meat and presence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Dogs; IFA; Risk factors.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300006

The importance of standardization in the quality of a scientific publication
Provedor de dados: 80
Autores: Fujita,Mariângela Spotti Lopes.
Tipo: Info:eu-repo/semantics/other
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300001

Thalassophryne nattereri fish venom: from the envenoming to the understanding of the immune system
Provedor de dados: 80
Autores: Lopes-Ferreira,Monica; Grund,Lidiane Zito; Lima,Carla.
Thalassophryne nattereri (niquim) is a venomous fish found off North and Northeast coast of Brazil, where it is known by the severity of the accidents involving humans. This review article is divided into four topics. The first one provides a brief description of the animal biology and its distribution off Brazilian coastal waters, the venom apparatus, signs and symptoms observed in envenomated humans and also describes envenomation in mice. The second topic describes the use of modern genetic approach and mass spectrometry for identification of highly expressed genes in its venom glands and the sequence of major toxins. The third chapter offers a detailed study of tissue injury induced by the venom and reveals the role of toxins that impair inflammation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venomous fish; Thalassophryne nattereri; Immunological memory; Nattectin; Natterin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200208

Clinical and cardiovascular alterations produced by scorpion envenomation in dogs
Provedor de dados: 80
Autores: Cordeiro,F. F.; Sakate,M.; Fernandes,V.; Cuyumjian,P. R.
Scorpionism is a common problem that occurs in tropical and subtropical countries and assumes great medical-sanitary importance due to its fatal effect on sensitive individuals, being able to lead children and aged people to death. The envenomation lethal potential is responsible for the serious cardiopulmonary alterations the scorpion toxin produces in its victims. The present research evaluated the effects of Tityus serrulatus venom on dogs, using two distinct doses: a dose that simulates natural envenomation (0.4 mg/total dose), and an experimental dose (0.25 mg/kg). General clinical signs were observed at different moments after envenomation, and specific data related to the cardiopulmonary system were evaluated by systemic arterial pressure...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion; Dogs; Cardiopulmonary alterations; Tityus serrulatus.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100003

Clinical and biochemical manifestation produced by scorpion (Hemiscorpius lepturus) venom in experimental animals
Provedor de dados: 80
Autores: Mirakabbadi A.,Zare; Zolfagharian,H.; Hedayat,A.; Jalali,A.
Several studies have been published about the clinical and biochemical manifestations produced by the venom of scorpions of the Buthidae family, but very few reports have indicated the manifestations induced by the venom of the Scorpionidae family. Hemiscorpius lepturus is an important scorpion species present in the south and southwestern part of Iran, causing morbidity and mortality in children and adults. For the present study, H. lepturus venom was extracted by electric shock and subcutaneously injected (6.3mg/kg) into a group of six rabbits. Blood collection was carried out before and three hours after venom injection for determination of osmotic fragility and levels of blood sugar, alanine aminotransferase (ALT), aspartate aminotransferase (AST),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemiscorpius lepturus; Scorpions; Osmotic fragility; Iran; Biochemical changes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400007

Cell-free antigens from precocious Paracoccidioides brasiliensis culture induce a typical delayed-type hypersensitivity reaction
Provedor de dados: 80
Autores: CJ,Fecchio; LA,Dias-Melicio; AMVC,Soares; S,Guimarães; MTS,Peraçoli; A,Sartori.
Cell-free antigens (CFAg) derived from Paracoccidioides brasiliensis have typically been used in immunodiffusion reactions for serodiagnosis or therapeutic follow-up of paracoccidioidomycosis patients. Thus, we investigated the usefulness of CFAg obtained from cultures at different ages, to evaluate cellular immunity by the footpad test, in experimental murine paracoccidioidomycosis. Male mice infected with P. brasiliensis 265 strain were challenged in the footpad with CFAg obtained from four- (4d CFAg) or 11-day-old cultures (11d CFAg). The increase in footpad swelling provoked by 4d CFAg and 11d CFAg was similar and showed significant difference in relation to control groups. However, the infiltrate pattern was strikingly different: 4d CFAg induced a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paracoccidioides brasiliensis; Cell-free antigens; Delayed-type hypersensitivity reaction; Gp43; Murine paracoccidioidomycosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300012

Hymenoptera marking technique
Provedor de dados: 80
Autores: Pereira,A. M.; Chaud-Netto,J.
In true social hymenopterans, such as many species of bees, wasps and all species of ants, the main characteristics are the overlapping of generations, the care with the offspring and the division of labor among the members of the colony. The first biological feature means that in a same moment there are groups of individuals, with variable ages, that execute different activities in the colony. In order to study the division of labor among the members of the colony, or to estimate the life span of these insects, or even to analyze any kind of behavior in non-social insects, it is necessary to know the exact age of each individual. For this reason, the insects must be identified soon after emergence. The identification of insects with numbers is an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apis mellifera; Hymenoptera; Marking technique; Social insects.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100014

Intraspecific variation in protein pattern of red scorpion (Mesobuthus tamulus, coconsis, pocock) venoms from Western and Southern India
Provedor de dados: 80
Autores: Badhe,R. V.; Thomas,A. B.; Harer,S. L.; Deshpande,A. D.; Salvi,N.; Waghmare,A.
Red scorpions Mesobuthus tamulus (Coconsis, Pocock) were obtained from different regions of West and South India (Ratnagiri, Chiplun and Ahmednagar from Maharashtra and Chennai from Tamil Nadu, respectively). Their venoms composition was analyzed using gel electrophoresis (SDS-PAGE). All venom samples shared six bands of 170, 80, 60, 57, 43, and 38 kDa molecular weights. Bands of 115 kDa and 51.5 kDa were characteristic of venoms obtained from red scorpions of Chiplun region, and the 26kDa band was absent in scorpion venom from Tamil Nadu. The separated protein band patterns suggest that the venoms from Ratnagiri, Ahmednagar and Tamil Nadu had high similarities in their biochemical composition but differed from that of Chiplun region. These data were also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Red scorpion; SDS-PAGE; Mesobuthus tamulus.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400008

Decrease in hepatitis B prevalence among blood donors in Central-West Brazil
Provedor de dados: 80
Autores: Lindenberg,Andréa de Siqueira Campos; Motta-Castro,Ana Rita Coimbra; Puga,Marco Antonio; Ortiz Tanaka,Tayana Serpa; Torres,Marina Sawada; Fernandes-Fitts,Sonia Maria; Cunha,Rivaldo Venancio.
Background The aim of the present study was to estimate hepatitis B virus seroprevalence among first-time blood donors in the city of Campo Grande, Mato Grosso do Sul State, in the central-western region of Brazil. Findings A retrospective analysis of first-time voluntary blood donor records, from January 2010 to December 2010, was conducted at the Hematology Center of Mato Grosso do Sul. The prevalence of the HBsAg and anti-HBc serological markers and their respective 95% confidence intervals were calculated. Chi-square analysis was performed between the seroprevalence previously found in 2001 and the one determined by the current study. Results were considered statistically significant if p &lt; 0.05. Among 8,840 subjects, 269 (3.04%, 95% CI: 2.7-3.4)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hepatitis B virus; Prevalence; Blood donors.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100402

Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice
Provedor de dados: 80
Autores: Machado,JG; Hoffmann,JL; Krause,VLK; da Silva,AV; Dias-Melicio,LA; Langoni,H.
Leishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-γ, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Immunosuppression; Immunopathology; Mice; Interleukins.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100013

Retrospective seroepidemiological analysis of patients with suspicion of paracoccidioidomycosis in São Paulo State, Brazil
Provedor de dados: 80
Autores: Kamikawa,CM; Kohara,VS; Passos,AN; Vicentini,AP.
The geographic distribution of paracoccidioidomycosis (PCM) in the Brazilian state of São Paulo was evaluated in a retrospective study using secondary data from serological analyses, carried out by double immunodiffusion assay of patients with PCM suspicion, from January 1999 to May 2010. Sixty percent of 10,176 patients, from 239 cities, were serologically reactive to P. brasiliensis. The cities that showed the most serological reactivity among patients were São João da Boa Vista (85%), Piracicaba (75%), Sorocaba (73%), Campinas (72%) and São Paulo (62%). São Paulo state has an area of 248,209.4 km²; the climate is tropical and sub-tropical with annual temperatures between 18 and 24ºC, high rainfall (900 to 1800 mm/year), rainy summers and mild winters....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paracoccidioides brasiliensis; Paracoccidioidomycosis; Immunological tests; Seroepidemiological studies.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300003

Paracoccidioidomycosis surveillance and control
Provedor de dados: 80
Autores: Mendes,RP.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200003

Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines
Provedor de dados: 80
Autores: Silva,Cássio Prinholato da; Costa,Tássia R.; Paiva,Raquel M. Alves; Cintra,Adélia C. O.; Menaldo,Danilo L.; Antunes,Lusânia M. Greggi; Sampaio,Suely V.
Abstract  Background  Phospholipases A 2 (PLA 2 s) are abundant components of snake venoms that have been extensively studied due to their pharmacological and pathophysiological effects on living organisms. This study aimed to assess the antitumor potential of BthTX-I, a basic myotoxic PLA 2isolated from Bothrops jararacussu venom, by evaluating in vitro processes of cytotoxicity, modulation of the cell cycle and induction of apoptosis in human (HL-60 and HepG2) and murine (PC-12 and B16F10) tumor cell lines.  Methods  The cytotoxic effects of BthTX-I were evaluated on the tumor cell lines HL-60 (promyelocytic leukemia), HepG2 (human hepatocellular carcinoma), PC-12 (murine pheochromocytoma) and B16F10 (murine melanoma) using the MTT method. Flow cytometry...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararacussu; BthTX-I; Antitumor potential; Apoptosis; Cell cycle alterations.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100348

The action of red scorpion (Mesobuthus tamulus coconsis, pocock) venom and its isolated protein fractions on blood sodium levels
Provedor de dados: 80
Autores: Badhe,R. V.; Thomas,A. B.; Deshpande,A. D.; Salvi,N.; Waghmare,A.
Red scorpion (Mesobuthus tamulus or Buthus tamulus) venom samples were collected at different regions of India: western (Chiplun and Ahmednagar from Maharashtra State) and southern (Ratnagiri and Chennai from Tamil Nadu State). The action of whole venoms on the blood sodium levels of mice was assessed using flame photometry. Seven peptides were common to all venom samples. They were separated using the native polyacrylamide gel electrophoresis (PAGE) technique and their activities were also studied using flame photometry. There was a decrease in the concentration of sodium ions in the serum, which suggested the blockage of such ions by scorpion venom toxins. Among the 10 protein bands isolated, the band at 79.6 kDa presented maximum activity in decreasing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Red scorpion; Buthus tamulus; Venom activity; Sodium ion concentration; Venom protein fraction; Pharmacological action.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100006

Evaluation of intestinal bacterial flora and HIV-1 infected individual's nutritional status, supplemented with soluble fiber and probiotic
Provedor de dados: 80
Autores: Trindade,E. B. S. M.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300015

Epidemiological characterization and prognostic factors in patients with confirmed cerebral cryptococcosis in central Taiwan
Provedor de dados: 80
Autores: Chen,Chang-Hua; Sy,Hiu-Ngar; Lin,Li-Jhen; Yen,Hua-Cheg; Wang,Shao-Hung; Chen,Wei-Liang; Chen,Yu-Min; Chang,Yu-Jun.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryptococcus neoformans; Cryptococcus gattii Cryptococcosis; Meningitis; Outcome; Risk Assessment.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100320

Effect of Curcuma zedoaria crude extract against tumor progression and immunomodulation
Provedor de dados: 80
Autores: Carvalho,FR; Vassão,RC; Nicoletti,MA; Maria,DA.
The aim of the present work was to study the effect of the crude extract of Curcuma zedoaria on peripheral blood cells and tumor progression in C57Bl/6J mice injected with B16F10 murine melanoma cells. The intraperitoneal therapy showed a significant increase in total white and red blood cell counts, a decrease in peritoneal cell number and tumor volume reduction, whereas the oral administration revealed a noteworthy augmentation only in total leukocyte count. These results contribute to evaluate the importance of alternative treatments that employ phytotherapic compounds against tumor progression and its possible immunomodulation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melanoma; Tumor; Hematology; Curcuma zedoaria.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200013

CEVAP - Present, past and future
Provedor de dados: 80
Autores: Barraviera,Silvia Regina C. Sartori.
Tipo: Info:eu-repo/semantics/report
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100001

Erratum
Provedor de dados: 80
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300018

Uveitis following anti-snake venom therapy
Provedor de dados: 80
Autores: Nayak,S. G.; Satish,R.; Nityanandam,S.; Thomas,R. K.
Snakebite is a common medical emergency in the tropics, causing multisystemic involvement. Ophthalmic manifestations after snakebite have included ptosis and ophthalmoplegia. Uveitis as an immunological complication following therapy with anti-snake venom (ASV) serum has been rarely reported in literature. We reported two patients who developed uveitis and acute renal failure following snakebite treatment with ASV serum. Both patients recovered uneventfully with appropriate therapy.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Snakebite; Uveitis; Anti-snake venom; Ptosis; Ophthalmoplegia.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100010

Intestinal parasitic infections in HIV/AIDS patients: epidemiological, nutritional and immunological aspects
Provedor de dados: 80
Autores: Amâncio,FAM; Pascotto,VM; Souza,LR; Calvi,SA; Pereira,PCM.
This study applied a socioeconomic questionnaire designed to evaluate the frequency of intestinal parasites and characterize epidemiological, nutritional, and immunological variables in 105 HIV/AIDS patients - with and without parasitic infections, attending the Day Hospital in Botucatu, UNESP, from 2007 to 2008. Body mass index was calculated and the following tests performed: parasitological stool examinations; eosinophil, IgE, CD4+ T and CD8+ T lymphocyte cell counts; albumin test; viral load measure; and TNF-α, IFN-γ, IL-2, IL-5 and IL-10 cytokine levels. Results were positive for parasitic intestinal infections in 12.4% of individuals. Most patients had good socioeconomic conditions with basic sanitation, urban dwellings, treated water supply and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV/AIDS; Enteroparasites; Nutrition; Immunology; Cytokines.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200013

Neonatal immune response of Brazilian beef cattle to vaccination with Clostridium botulinum toxoids types C and D by indirect ELISA
Provedor de dados: 80
Autores: Curci,VCM; Nogueira,AHC; Nobrega,FLC; Araujo,RF; Perri,SHV; Cardoso,TC; Dutra,IS.
Types C and D strains of Clostridium botulinum are commonly related to avian and mammalian botulism. Although there are numerous vaccine recommendations, little research has been conducted to indicate the real effectiveness of vaccine timing or the ideal immunization protocol for young beef calves. Four commercially available vaccines, two bivalent (Clostridium botulinum types C and D; vaccines 1 and 2) and two polyvalent (all Clostridium spp. including Clostridium botulinum types C and D; vaccines 3 and 4), that are currently used in Brazilian herds, were tested in order to verify the maternal immune response. One hundred cows, divided into four vaccinated groups and one unvaccinated group, were given a two-dose subcutaneous immunization, at day zero,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Clostridium botulinum; Neonatal immunity; Cattle; Botulism; Brazil.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300018

The concept of two-dimensional electrophoresis-guided purification proven by isolation of rhodocetin from Calloselasma rhodostoma (Malayan pit viper)
Provedor de dados: 80
Autores: Tang,MS; Vejayan,J; Ibrahim,H.
Two-dimensional gel electrophoresis (2DE) is an important tool for investigating the complexity of snake venom proteomes. Apart from applications based on whole proteome analysis, we suggest that 2DE can be used as an assay to guide the progress of protein purification. The aim of this study was to prove the feasibility of this concept by using it to purify rhodocetin from Calloselasma rhodostoma venom. Rhodocetin (α subunit) spot on the 2DE profile of C. rhodostoma venom was first identified and confirmed by mass spectrometry, with a molecular mass of 16 kDa and calculated pI of 5.16. Rhodocetin was subsequently purified by successive anion-exchange and gel filtration chromatography. Every peak from both chromatography profiles was collected and tested on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein fingerprinting; Liquid chromatography; Proteomics; Venom.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400011

Rabies virus in a pregnant naturally infected southern yellow bat (Lasiurus ega)
Provedor de dados: 80
Autores: Allendorf,SD; Albas,A; Cipriano,JRB; Antunes,JMAP; Appolinário,CM; Peres,MG; da Rosa,AR; Sodré,MM; Megid,J.
Current knowledge on bat lyssavirus infections in their native hosts is limited and little is known about the virulence, virus dissemination and transmission among free-living insectivorous bats. The present study is a brief description of rabies virus (RABV) dissemination in tissues of a naturally infected pregnant southern yellow bat (Lasiurus ega) and its fetuses, obtained by reverse-transcriptase polymerase chain reaction (RT-PCR). The RT-PCR was positive in samples from the brain, salivary gland, tongue, lungs, heart, kidneys and liver. On the other hand, the placenta, three fetuses, spleen, intestine and brown fat tissue tested negative. This research demonstrated the absence of rabies virus in the fetuses, thus, in this specific case, the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rabies; Bats; Vertical transmission; RT-PCR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200014

Appropriate antivenom doses for six types of envenomations caused by snakes in taiwan
Provedor de dados: 80
Autores: Chieh-Fan,C; Tzeng-Jih,L; Wen-Chi,H; Hua-Wei,Y.
Six of the 15 species of venomous snakes found in Taiwan are responsible for most of the clinically significant envenomations in the country. These species are: Trimeresurus mucrosquamatus, Trimeresurus stejnegeri, Naja atra, Bungarus multicinctus, Deinagkistrodon acutus and Daboia russelii siamensis, which together can be subdivided into three groups based on their venom effects. Primary treatment consists of rapid administration of appropriate antivenoms. The present study aimed to identify a proper dose of antivenom for each snake group as well as to describe hemorrhagic, neurotoxic, and mixed effects of their venoms. A retrospective chart review identified 72 snakebite cases referred to an emergency department. Data on epidemiology, examination...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antivenom; Venomous snakes; Snake envenomation; Neurotoxic venom; Hemorrhagic venom.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300009

The effects of low-level laser on muscle damage caused by Bothrops neuwiedi venom
Provedor de dados: 80
Autores: Dourado,DM; Matias,R; Almeida,MF; De Paula,KR; Vieira,RP; Oliveira,LVF; Carvalho,PTC.
The present study aimed to assess the effects of low-level laser (660 nm) on myonecrosis caused by the insertion of Bothrops neuwiedi venom in the gastrocnemius muscle of rats. Male Wistar rats were divided into three groups (n = 24 each): Group S (0.9% saline solution); Group V (venom) and Group VLLL (venom plus low-level laser). These categories were subdivided into four additional groups (n = 6) based on the euthanasia timing (3 hours, 24 hours, 3 days and 7 days). The groups V and VLLL were inoculated with 100 µL of concentrated venom (40 µg/mL) in the gastrocnemius muscle. The muscle was irradiated using a gallium-aluminum-arsenide laser (GaAlAs) at 35 mW power and 4 J/cm² energy density for 3 hours, 24 hours, 3 days or 7 days after venom inoculation....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myonecrosis; B. neuwiedi venom low-level laser.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300004

Wound infections secondary to snakebite in central Taiwan
Provedor de dados: 80
Autores: Huang,Li-Wen; Wang,Jiaan-Der; Huang,Jin-An; Hu,Sung-Yuan; Wang,Lee-Min; Tsan,Yu-Tse.
There are very few microbiological data on wound infections following snakebites. The objective of this study was to investigate the treatment of secondary infection following snakebites in central Taiwan. Microbiological data and antibiotic sensitivity of wound cultures were retrospectively analyzed from December 2005 to October 2007 in a medical center in central Taiwan. A total of 121 snakebite patients participated in the study. Forty-nine (40.5%) subjects were bitten by cobra (Naja atra); 34 of them had secondary infection, and 24 of them (70.6%) needed surgical intervention. Cobra bites caused more severe bacterial infection than other snakebites. Morganella morganii was the most common pathogen, followed by Aeromonas hydrophila and Enterococcus....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snakebite; Antivenom; Venomous; Cobra; Bacterial infection; Bacterial resistance; Taiwan.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300004

Occurrence of Cryptosporidium sp infection in indviduals infected by HIV, sick or not, treated at the University Hospital, Botucatu School of Medicine - UNESP
Provedor de dados: 80
Autores: Langoni Junior,G.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100022

Bats and their role in human rabies epidemiology in the Americas
Provedor de dados: 80
Autores: Dantas-Torres,F.
Bats are very interesting animals: they are the unique flying mammals, have developed a highly sophisticated echolocation system, and have become specialized to eat different types of diets. Hematophagous (vampire) bats are those specialized to feed solely on blood and have served as a source of inspiration for researchers as well as for writers. Vampire bat attacks on humans have moved from the realm of science fiction to reality in Latin America and bats (including non-hematophagous ones) have assumed an important role in the transmission of rabies virus to humans. This article discusses the emerging role of bats as rabies virus transmitters, with particular emphasis on the role of hematophagous bats in the epidemiology of human rabies in Latin America....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Epidemiology; Disease transmission; Chiroptera.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200002

Skin manifestations caused by Brazilian traumatic, allergenic, and venomous plants: main species, therapeutic and preventive measures
Provedor de dados: 80
Autores: Haddad Junior,V.
Brazilian flora is very rich and a large number of specimens can cause adverse reactions, from mild erythema to cutaneous necrosis. Plants or vegetal extracts are always suspected of contact dermatitis; they are found in all types of environments, including dwellings. Other harmful effects, which can be identified by clinical manifestations and the aid of the patient, are phytophotodermatitis, traumas, thorn infections, chemical irritations, or urticaria caused by Urtica sp. Knowledge on the most important plants and their effects on human skin are very useful, and diagnosis is very important in treatment of the complications.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian plants; Envenoming by plants; Anacardiacea; Phytophotodermatitis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300002

Post-exposure treatment of Ebola virus using passive immunotherapy: proposal for a new strategy
Provedor de dados: 80
Autores: Chippaux,Jean-Philippe; Boyer,Leslie V; Alagón,Alejandro.
Background Better treatments are urgently needed for the management of Ebola virus epidemics in Equatorial Africa. Methods We conducted a systematic review of the literature on the use of passive immunotherapy for the treatment or prevention of Ebola virus disease. We placed findings from this review into the context of passive immunotherapy currently used for venom-induced disease, and recent improvements in manufacturing of polyvalent antivenom products. Results Passive immunotherapy appears to be one of the most promising specific treatments for Ebola. However, its potential has been incompletely evaluated, considering the overall experience and recent improvement of immunotherapy. Development and use of heterologous serum derivatives could protect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ebola; Epidemics; Immunotherapy; Prophylaxis; Africa.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100203

Snakoid instead of venomoid
Provedor de dados: 80
Autores: Gawade,S. P.
Tipo: Info:eu-repo/semantics/other
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200002

New additions to the scorpion fauna of Riyadh region, Saudi Arabia
Provedor de dados: 80
Autores: Al-Asmari,AK; Al-Saief,AA; Abdo,NM; Al-Moutaery,KR.
In this work, we present additional morphological data about the scorpion fauna of Saudi Arabia. This investigation was carried out in the central region (Riyadh) of the country and identified existing and newly discovered scorpion species for taxonomic documentation while determining the medically important ones. The survey covered the entire Riyadh region, including all major districts, and collected a total of 4,164 specimens. Morphological identification of collected animals was based on identification keys. There were two species (one with a subspecies) that belonged to the family Scorpionidae, namely Scorpio maurus kruglovi (0.02%) and Hemiscorpius arabicus (0.05%). The latter, currently, is part of the Hemiscorpiidae family that had been upgraded...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saudi Arabia; Riyadh; Hemiscorpius; Leiurus; Androctonus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400003

Ethnomedicinal plants used for snakebite treatments in Ethiopia: a comprehensive overview
Provedor de dados: 80
Autores: Yirgu,Abraham; Chippaux,Jean-Philippe.
Abstract Traditional medicine plays an important role in the daily lives of people living in rural parts of Ethiopia. Despite the fact that Ethiopia has a long history of using traditional medicinal plants as an alternative medicine source, there is no checklist compiling these plants used for snakebite treatment. This review collected and compiled available knowledge on and practical usage of such plants in the country. A literature review on medicinal plants used to treat snakebites was conducted from 67 journal articles, PhD dissertation and MSc theses available online. Data that summarize scientific and folk names, administration methods, plant portion used for treatment and method of preparation of recipes were organized and analyzed based on citation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethnobotany; Medicinal plant; Traditional treatment; Snakebite; Envenomation; Sub-Saharan Africa; Ethiopia.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100205

Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom
Provedor de dados: 80
Autores: Lucas de Oliveira,P. C.; Sakate,M.; Madruga,R. A.; Barbosa,N. P.U.
Venoms from snakes of the Bothrops genus are proteolytic, coagulant, hemorrhagic and nephrotoxic, causing edema, necrosis, hemorrhage and intense pain at the bite site, besides systemic alterations. Many adjuvants have been added to the venom used in the sensitization of antiserum-producer animals to increase antigenic induction and reduce the envenomation pathological effects. Gamma radiation from 60Co has been used as an attenuating agent of the venoms toxic properties. The main objective was to study, comparatively, clinical and laboratory aspects of goats inoculated with bothropic (Bothrops jararaca) venom, natural and irradiated from a 60Co source. Twelve goats were divided into two groups of six animals: GINV, inoculated with 0.5mg/kg of natural...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiation; Bothrops jararaca; Cobalt; Goats.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300003

Cytokines and acute-phase serum proteins as markers of inflammatory regression during pulmonary tuberculosis treatment
Provedor de dados: 80
Autores: Peresi,E.
Tipo: Info:eu-repo/semantics/other
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100019

Immunotherapeutic potential of Crotoxin: anti-inflammatory and immunosuppressive properties
Provedor de dados: 80
Autores: Sartim,Marco Aurélio; Menaldo,Danilo Luccas; Sampaio,Suely Vilela.
Abstract For the past 80 years, Crotoxin has become one of the most investigated isolated toxins from snake venoms, partially due to its major role as the main toxic component in the venom of the South American rattlesnake Crotalus durissus terrificus. However, in the past decades, progressive studies have led researchers to shift their focus on Crotoxin, opening novel perspectives and applications as a therapeutic approach. Although this toxin acts on a wide variety of biological events, the modulation of immune responses is considered as one of its most relevant behaviors. Therefore, the present review describes the scientific investigations on the capacity of Crotoxin to modulate anti-inflammatory and immunosuppressive responses, and its application as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durissus terrificus; Crotoxin; Innate immunity; Adaptative immunity; Inflammation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100329

Determination of disulfide bridges of two spider toxins: hainantoxin-III and hainantoxin-IV
Provedor de dados: 80
Autores: Wang,W; Liu,Z; Qian,W; Fang,Y; Liang,S.
Peptide toxins are usually highly bridged proteins with multipairs of intrachain disulfide bonds. Analysis of disulfide connectivity is an important facet of protein structure determination. In this paper, we successfully assigned the disulfide linkage of two novel peptide toxins, called HNTX-III and HNTX-IV, isolated from the venom of Ornithoctonus hainana spider. Both peptides are useful inhibitors of TTX-sensitive voltage-gated sodium channels and are composed of six cysteine residues that form three disulfide bonds, respectively. Firstly, the peptides were partially reduced by tris(2-carboxyethyl)-phosphine (TCEP) in 0.1 M citrate buffer containing 6 M guanidine-HCl at 40° C for ten minutes. Subsequently, the partially reduced intermediates containing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disulfide bonds; TCEP; Partial reduction; HNTX-III; HNTX-IV.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200009

Delayed double reading of whole blood clotting test (WBCT) results at 20 and 30 minutes enhances diagnosis and treatment of viper evenomation
Provedor de dados: 80
Autores: Benjamin,Jordan Max; Chippaux,Jean-Philippe; Sambo,Bio Tamou; Massougbodji,Achille.
Abstract  Background The whole blood clotting test (WBCT) is a simple test of coagulation that is often used in the assessment, diagnosis, and therapeutic monitoring of snakebite patients in sub-Saharan Africa. WBCT requires only a clean glass tube and several milliliters of venous blood and is ideal for use in poorly equipped health centers throughout the rural areas where 95% of snakebites occur. However, questions surrounding the accuracy and reliability of the test remain unanswered due to variations in testing conditions and a lack of comparative research with which to validate them. This is the first study to evaluate WBCT results at both 20-min (WBCT20) and 30-min (WBCT30) reading times in the same group of snakebite patients.  Methods In order to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Africa; Snakebite; Echis; Envenomation; Whole blood clotting test; WBCT; Venom-induced consumption coagulopathy; Carpet viper; Saw-scaled viper.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100308

First report of brown widow spider sightings in Peninsular Malaysia and notes on its global distribution
Provedor de dados: 80
Autores: Muslimin,Mustakiza; Wilson,John-James; Ghazali,Amir-Ridhwan M; Braima,Kamil A; Jeffery,John; Wan-Nor,Fitri; Alaa-Eldin,Mohamed E; Mohd-Zin,Siti-Waheeda; Wan-Yusoff,Wan S; Norma-Rashid,Yusoff; Lau,Yee L; Rohela,Mahmud; Abdul-Aziz,Noraishah M.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: &lt; Italic&gt; Latrodectus geometricus&lt; /italic&gt; Brown widow spider Colonization DNA barcoding Envenomation Global invasion Invasive species Medically important arthropods Synanthropy.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100318

Effects of the European hornet ( Vespa crabro Linnaeus 1761) crude venom on its own species
Provedor de dados: 80
Autores: Nadolski,Jerzy.
Background Lethal dose 50% is a classical index of toxicity that usually employs small rodents as experimental animals. Therefore, scarce data are available on the effects of venom on invertebrates, particularly the impact of wasp venom on its own species. Findings In the present study, the lethality of Vespa crabro venom on its own species was studied. Lethal dose 50% values of crude venom on workers of hornet Vespa crabro were estimated to be 4.0 mg/kg of body weight. Conclusions Wasps can use their venom apparatus effectively when attacking foreign workers that appear in the immediate vicinity of their nest. The toxins released during stinging are potent enough to kill. The result of this study eliminates the popular myth that venomous animals can be...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: European hornet; Vespa crabro; Hornet venoms; LD50.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100401

Efficacy of sertraline against Trypanosoma cruzi: an in vitro and in silico study
Provedor de dados: 80
Autores: Ferreira,Daiane Dias; Mesquita,Juliana Tonini; Silva,Thais Alves da Costa; Romanelli,Maiara Maria; Batista,Denise da Gama Jaen; Silva,Cristiane França da; Gama,Aline Nefertiti Silva da; Neves,Bruno Junior; Melo-Filho,Cleber Camilo; Soeiro,Maria de Nazare Correia; Andrade,Carolina Horta; Tempone,Andre Gustavo.
Abstract  Background: Drug repurposing has been an interesting and cost-effective approach, especially for neglected diseases, such as Chagas disease.  Methods: In this work, we studied the activity of the antidepressant drug sertraline against Trypanosoma cruzi trypomastigotes and intracellular amastigotes of the Y and Tulahuen strains, and investigated its action mode using cell biology and in silico approaches.  Results: Sertraline demonstrated in vitro efficacy against intracellular amastigotes of both T. cruzi strains inside different host cells, including cardiomyocytes, with IC50 values between 1 to 10 μM, and activity against bloodstream trypomastigotes, with IC50 of 14 μM. Considering the mammalian cytotoxicity, the drug resulted in a selectivity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Drug; Treatment; Sertraline; Drug repurposing; Drug repositioning.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100321

Cathepsin B/X is secreted by Echinometra lucunter sea urchin spines, a structure rich in granular cells and toxins
Provedor de dados: 80
Autores: Sciani,Juliana Mozer; Antoniazzi,Marta Maria; Neves,Adriana da Costa; Pimenta,Daniel Carvalho.
Background Echinometra lucunter is a common American sea urchin responsible for the majority of the marine accidents in Brazil. Although not lethal, these accidents are reported to be extremely painful. Recently, our group described the presence of toxins in its spines that contribute to the pathological reactions. Additionally, we have observed that the E. lucunter spines can regenerate when broken. In the present work we evaluated the enzymatic activities of sea urchin spine extracts in order to identify an enzyme that could contribute not only to the toxicity, but also participate in the spine growth and regeneration. Results The spine aqueous extract was tested for peptidase activity, with synthetic substrates, in the presence and absence of inhibitors...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Echinometra lucunter; Spines; Cathepsin; Proteolysis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100318

The importance of serological assays in diagnosing acute pulmonary histoplasmosis
Provedor de dados: 80
Autores: Freitas,RS; Carvalho-Vivi,JO; Zamboni,IM; Assis,CM; Costa-Martins,JE; Vicentini-Moreira,AP.
Histoplasmosis is a systemic mycosis caused by inhalation of Histoplasma capsulatum microconidia. The disease does not normally affect immunocompetent individuals after a single, transient inhalation exposure. However, longer exposure may cause chronic or disseminated acute pulmonary infection. Herein, we report the case of a 24-year-old immunocompetent patient, who presented fever, cough and dyspnea for one month. The chest radiography revealed interstitial infiltrate and diffuse micronodules. The patient reported having had close and prolonged contact with bats. Diagnosis was confirmed by positive double immunodifusion and immunoblotting assays. She was treated with ketoconazole (400 mg) and there was complete resolution of the disease.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Histoplasmosis; Histoplasma capsulatum; Immunocompetence; Serodiagnosis; Bats.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200010

Viper bites complicate chronic agrochemical nephropathy in rural Sri Lanka
Provedor de dados: 80
Autores: Silva,Anjana; Samarasinghe,Rivikelum; Pilapitiya,Senaka; Dahanayake,Niroshana; Siribaddana,Sisira.
Snakebite is a common occupational health hazard among Sri Lankan agricultural workers, particularly in the North Central Province. Viperine snakes, mainly Russell’s viper envenomation, frequently lead to acute renal failure. During the last two decades, an agrochemical nephropathy, a chronic tubulointerstitial disease has rapidly spread over this area leading to high morbidity and mortality. Most of the epidemiological characteristics of these two conditions overlap, increasing the chances of co-occurrence. Herein, we describe four representative cases of viperine snakebites leading to variable clinical presentations, in patients with chronic agrochemical nephropathy, including two patients presented with acute and delayed anuria. These cases suggest the...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Snakebite; Chronic kidney disease; Agricultural nephropathy; Epidemiology; Anuria.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200505

Bacterial contamination of the catfish (Cathorops agassizii - Valenciennes, 1839 and Genidens genidens - Agassiz, 1829) sting
Provedor de dados: 80
Autores: Junqueira,Marcos Emerson Pinheiro.
Tipo: Info:eu-repo/semantics/article
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300015

Hepatitis B virus: evaluation of infection risk and serological response to vaccine in hospital cleaners of the Botucatu School of Medicine
Provedor de dados: 80
Autores: Osti,C.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200010

Antibacterial activity of propolis collected in different regions of Brazil
Provedor de dados: 80
Autores: Gonsales,G. Z.; Orsi,R. O.; Fernandes Júnior,A.; Rodrigues,P.; Funari,S. R. C.
The aim of this study was to investigate the antibacterial activity of propolis samples from Goiás, Paraná and São Paulo States, Brazil, and their flavonoids content. Ethanolic extracts of propolis (EEP) were prepared (30g of propolis in 70% ethanol), and the microorganisms Staphylococcus aureus and Escherichia coli were tested. The methodology employed was agar diffusion using filter paper discs. Ampicillin and tetracycline were used as controls. Antibacterial activity was determined by the reading of inhibition zone diameters (mm) after 24 hours incubation at 37°C. Results demonstrated that EEP inhibited the growth of Staphylococcus aureus but not that of Escherichia coli. Tetracycline and ampicillin showed an efficient action against both bacteria....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propolis; Antibacterial activity; Flavonoids.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200009

Identification and antimicrobial susceptibility of Staphylococcus from home-treated peritoneal dialysis patients
Provedor de dados: 80
Autores: Batalha,JEN; Cunha,MLRS; Montelli,AC; Barreti,P; Caramori,JCT.
Staphylococcus aureus is the main agent of infections during peritoneal dialysis (PD). The presence of S. aureus in the nasal cavity has been extensively studied and suggested as a risk factor of dialysis-related infections, whereas coagulase-negative Staphylococcus (CNS) species are frequently considered part of the normal human microbiota. The aim of this study was to identify Staphylococcus in the nasal cavity, pericatheter skin and peritoneal effluent from PD patients, as well as to evaluate the antimicrobial activity evolution in vitro. Thirty-two chronic PD patients were observed during 12 months and had nasal and pericatheter skin samples collected for culture. When peritonitis was detected, samples were also collected from the peritoneal effluent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peritoneal dialysis (PD); Peritonitis; Risk factor; Staphylococcus; Coagulase-negative Staphylococcus (CNS).
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200005

Assessment of biomedical and pharmacological activities of sea anemones Stichodactyla mertensii and Stichodactyla gigantea from Gulf of Mannar Biosphere Reserve, southeast coast of India
Provedor de dados: 80
Autores: Thangaraj,S; Bragadeeswaran,S.
Cnidarians comprise an old and diverse animal phylum, and possess a wide variety of biologically active substances. Sea anemones contain a diversity of interesting biologically active compounds including some potent toxins. In the present work, the sea anemones Stichodactyla mertensii and Stichodactyla gigantea, collected from the Mandapam coast, are characterized biomedically and pharmacologically. The crude protein was obtained by using methanol and aqueous extracts. The respective protein contents of S. mertensii and S. gigantea were found to be 2.10 µg/mL and 1.87 µg/mL. The methanol and aqueous extracts of S. mertensii and S. gigantea yielded six and nine bands by SDS-PAGE on 12% gel. In the hemolytic assay, both extracts exhibited hemolytic effect on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aqueous extract; Neurotoxicity; Mouse bioassay; Analgesic activity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100007

Stings from Euscorpius flavicaudis (De Geer, 1778) (Scorpiones, Euscorpiidae) during pregnancy: a clinical case report
Provedor de dados: 80
Autores: Dutto,M; Dutto,L; Bertero,M; Goyffon,M.
We report a case in which a 21-week pregnant woman was stung by a Euscorpius flavicaudis (De Geer, 1778) scorpion. Symptoms and signs experienced by the patient were the same as those documented in the literature and with no ill-effects for the pregnancy. Envenoming was local and of low degree of intensity. It is important to emphasize that the patient was stung in her home, which differs from stings in most other parts of the world, in which scorpionism is mostly a risk in outdoor areas.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Euscorpius flavicaudis; Sting; Scorpionism; Pregnant woman; Clinical; Symptoms.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400018

Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera
Provedor de dados: 80
Autores: Ferreira Junior,R. S.; Nascimento,N.; Martinez,J. C.; Alves,J. B.; Meira,D. A.; Barraviera,B.
ELISA was used to evaluate, follow, and compare the humoral immune response of Swiss mice during hyperimmunization with natural and Cobalt 60-irradiated (60Co) Crotalus durissus terrificus venom. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by "in vivo" challenge and the side effects were assessed. The animals immunization with one LD50 of the venom was on days one, 15, 21, 30, and 45, when blood samples were collected; the challenges occurred on the 60th day. Results showed that ELISA was efficient in evaluating, following, and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperimmunization; Irradiation; Crotalus durissus terrificus; ELISA; Serum neutralization; Detoxification.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300008

Leptospira spp. infection in sheep herds in southeast Brazil
Provedor de dados: 80
Autores: Barbante,Priscila; Shimabukuro,Fabio H; Langoni,Helio; Richini-Pereira,Virgínia B; Lucheis,Simone B.
Background With the aim of studying Leptospira spp. infection in sheep herds, blood samples and respective kidney and liver fragments were collected from 100 animals from twenty different properties during slaughter at a meat company in the Sorocaba region, São Paulo state, southeast Brazil. The microscopic agglutination test (MAT) was performed with 29 strains of Leptospira spp. To identify the agent in the liver and kidney, 100 samples of each tissue were submitted to culture in Fletcher medium and analyzed by the polymerase chain reaction (PCR) for Leptospira spp.Results MAT detected 23 samples serologically positive for one or more Leptospira spp. serovars and significantly more for Autumnalis. Eight (4%) samples were positive in culture (four kidneys...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Ovine; Serology; Culture; PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200325

Snakebite in Bobo-Dioulasso, Burkina Faso: illustration of realities and challenges for care based on a clinical case
Provedor de dados: 80
Autores: Kyelem,CG; Yaméogo,TM; Ouédraogo,SM; Zoungrana,J; Poda,GEA; Rouamba,MM; Ouangré,A; Kissou,SA; Rouamba,A.
We report herein the case of 19-year-old female farmer who suffered a double snakebite on the right foot. After an unsuccessful traditional treatment, she consulted a health center, 48 hours after the bite. Upon arrival at the hospital, she showed signs of severe damage, including hemorrhagic syndrome, extensive gangrene of the bitten limb and severe acute renal failure. Due to financial constraints, neither antivenom nor the scheduled amputation was performed. After 35 days of hospitalization, she returned home, against the advice of medical personnel. Our case summarizes the daily challenges of patients and practitioners that suffer snakebite envenomation in Bobo-Dioulasso, western Burkina Faso.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Envenomation; Snakebite; Management; Burkina Faso; Sub-Saharan Africa.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400021

An idea comes true...
Provedor de dados: 80
Autores: Barraviera,Silvia Regina C. Sartori; Barraviera,Benedito.
Tipo: Info:eu-repo/semantics/article
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200001

Survival of a newborn from a pregnant woman with rabies infection
Provedor de dados: 80
Autores: Zhen-Yu,Qu; Guo-Wei,Li; Qiao-Ge,Chen; Peng,Jiang; Chang,Liu; Alfred,Lam.
Abstract  Background  Rabies is very common in People’s Republic of China. Each year thousands of people die because of this disease, but rabies diagnosed in pregnancy is very rare.  Case Presentation  In this study, we report the case of a pregnant woman who was infected with the rabies virus after a dog bite. The symptoms of rabies appeared in labor and she died after pregnancy. Her baby and husband did not develop the disease.  Conclusion  The phenomenon that the newborn infant was healthy may be related to the protective role of placenta in resisting the invasion of the rabies virus or the absence of systemic viremia. The prompt administration of vaccines and anti-rabies immunoglobulin to the infant may have also contributed to his survival.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Rabies; Pregnant; Newborn baby.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100502

New reports on parasitism by Haplometroides buccicola (Digenea, Plagiorchiidae) in Brazilian snakes
Provedor de dados: 80
Autores: Santos,KR; Barrella,TH; Zica,EOP; Silva,RJ.
The occurrence of Haplometroides buccicola (Digenea, Plagiorchiidae) in the esophagus of two Brazilian snakes is reported in the present study. The trematodes were collected from one Micrurus corallinus (Elapidae) and one Phalotris lativittatus (Colubridae); both snakes were found in Botucatu city, São Paulo State, Brazil. Morphological and morphometric analyses of the trematodes are presented. For the first time Micrurus corallinus has been recorded as a host for H. buccicola and this is the second time that P. lativittatus has been reported as a host for this trematode species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haplometroides buccicola; Plagiorchiidae; Phalotris lativittatus; Micrurus corallinus; Snakes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300011

Epidemiology of bee stings in Campina Grande, Paraíba state, Northeastern Brazil
Provedor de dados: 80
Autores: Linard,Ana Thaise Sousa; Barros,Rafaella Moreno; Sousa,Jorge Alves; Leite,Renner Souza.
Background : The present study aims to investigate the clinical-epidemiological characteristics of bee sting cases recorded between 2007 and 2012 in the city of Campina Grande, Paraiba state, Brazil. Data were collected from the database of the Injury Notification Information System of the Brazilian Ministry of Health.Results : A total of 459 bee sting cases were retrospectively analyzed. The average annual incidence was 19 cases per 100,000 inhabitants. Cases were distributed in all months of the year, with higher prevalence in September and February. Most victims were men aged between 20 and 29 years. The highest incidence of cases was recorded in urban areas. Victims were stung mainly on the head and torso and received medical assistance predominantly 1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Africanized honeybee; Paraiba; Human envenomation; Venomous animals; Public health.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200324

The evolution of clinical research and the open registration
Provedor de dados: 80
Autores: Barraviera,Benedito.
Tipo: Info:eu-repo/semantics/article
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400001

IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: cross-reaction with venoms from Bothrops species from Argentina
Provedor de dados: 80
Autores: Rodríguez,JP; De Marzi,MC; Maruñak,S; Teibler,P; Acosta,O; Malchiodi,EL; Leiva,LC.
We examined the ability of IgG anti-crotalic PLA2 to cross-react with Bothrops spp. venoms, from snakes found in the northeast of Argentina. Immunoblotting and ELISA tests showed that IgG anti-crotalic PLA2 recognize antigens of bothropic venoms. Indirect hemolytic activity tests showed that the quantity of antibodies that neutralized 50% of Crotalus durissus terrificus venom (ED50: 2.1 mg IgG anti-crotalic PLA2/100 µg of venom) were also able to neutralize venom from other snakes in the following proportion: 34% of B. alternatus, 18% of B. diporus and 12% of B. jararacussu. Likewise, direct PLA2 activity neutralization tests showed a similar cross-neutralization pattern including 56% of B. alternatus, 29% of B. diporus and 30% of B. jararacussu. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops; Crotalus; PLA2; Neutralization; Cross-reaction.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300008

Clinical and electrocardiographic evaluation during experimental toad poisoning in dogs
Provedor de dados: 80
Autores: Camplesi,AC; Sakate,M; Simão,NMB; Marucio,R; Mota,FCD; Moya-Araujo,CF.
Accidents involving toad poisoning are frequent and dogs are the most common victims; they become poisoned by biting or ingesting a toad. When released in the organism, the venom is absorbed by both the oral mucosa and the digestive tract, initiating its toxic action. The aim of this work was to evaluate the clinical and electrocardiographic aspects of dogs subjected to experimental toad poisoning, as well as their response to treatment with propranolol. Twenty dogs were divided into two groups, a control group (n = 5) and a poisoned group (n = 15). After general anesthesia, the control group received a placebo, while the poisoned group received a venom aliquot through an orogastric tube. Results were tested through multivariate analysis (p &lt; 0.05). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dogs; Toad venom; Poisoning; Bufotoxin; Arrhythmias.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200014

Use of SBA - 15 nanostructured silica as adjuvant in the immunization process of young ovines with natural and cobalt 60-irradiated Crotalus durissus terrificus venom
Provedor de dados: 80
Autores: Anderlini,R. P.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200019

Deep sequencing analysis of toad Rhinella schneideri skin glands and partial biochemical characterization of its cutaneous secretion
Provedor de dados: 80
Autores: Shibao,Priscila Yumi Tanaka; Cologna,Camila Takeno; Morandi-Filho,Romualdo; Wiezel,Gisele Adriano; Fujimura,Patricia Tiemi; Ueira-Vieira,Carlos; Arantes,Eliane Candiani.
Abstract  Background: Animal poisons and venoms are sources of biomolecules naturally selected. Rhinella schneideri toads are widespread in the whole Brazilian territory and they have poison glands and mucous gland. Recently, protein from toads’ secretion has gaining attention. Frog skin is widely known to present great number of host defense peptides and we hypothesize toads present them as well. In this study, we used a RNA-seq analysis from R. schneideri skin and biochemical tests with the gland secretion to unravel its protein molecules.  Methods: Total RNA from the toad skin was extracted using TRizol reagent, sequenced in duplicate using Illumina Hiseq2500 in paired end analysis. The raw reads were trimmed and de novo assembled using Trinity. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNA-seq; Rhinella schneideri; Toad secretion; Transcriptome; Illumina; Cutaneous secretion; Skin secretion; Toad protein.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100326

Comparison of different diagnostic tests in dogs uninfected and naturally infected with visceral leishmaniasis
Provedor de dados: 80
Autores: Sassaki,CY; Colodel,MM; Ferreira,I; Nogueira,FS; Lucheis,SB; Langoni,H; Rocha,NS.
Uninfected dogs (n = 10) and those naturally infected with leishmaniasis (n = 10) were subjected to several diagnostic tests, namely: hemoculture, polymerase chain reaction (PCR) of hemoculture, indirect immunofluorescence (RIFI), cytological examination of lymph node aspirate, culture of lymph node aspirate and PCR of lymph node aspirate. RIFI - followed by PCR of lymph node aspirate culture - presented more positive results in infected dogs than in uninfected ones. In infected animals, RIFI was more effective than PCR of lymph node aspirate culture. There was no statistical difference in positivity between RIFI and hemoculture; lymph node aspirate culture/cytological examination of lymph node aspirate and PCR of hemoculture; and between PCR of lymph node...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Dogs; Visceral leishmaniasis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300015

Documented bites by a yellow sac spider (Cheiracanthium punctorium) in Italy: a case report
Provedor de dados: 80
Autores: Papini,R.
In Italy reports of human envenomations by yellow sac spiders have been sporadic. Since increasing clinical information would improve understanding of the danger of yellow sac spiders to humans, we report the case of a 7-year-old child and her father bitten by a documented Cheiracanthium punctorium. They developed acute persistent pain with local skin signs and numbness, and required emergency treatment. The father recovered completely within 1 to 2 hours and the child within 3 to 4 days after treatment, probably as a result of spontaneous evolution. Clinicians should be aware of the risks and immediate management of spider bites.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Spiders; Arachnidism; Italy.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300014

Lectures
Provedor de dados: 80
Tipo: Info:eu-repo/semantics/other
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200015

Production of Trichophyton mentagrophytes antigens and their characterization in mice
Provedor de dados: 80
Autores: Venturini,J; Rossi-Ferreira,R; Arruda,MSP.
The participation of dermatophytic antigens in the host-parasite balance is still poorly understood. One of the difficulties encountered by researchers is the lack of dominant and specific antigens that can be used in such studies. In order to contribute to a better understanding of this aspect of infection, the present study identifies antigen fractions obtained from exoantigen and cytoplasmic extracts of Trichophyton mentagrophytes. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) revealed the presence of 13 proteins in the exoantigen extract, whose molecular weight ranged from 12.5 to 90 kDa. The cytoplasmic extract contained 18 protein fractions ranging from 11 to 110 kDa. Immunoblotting showed the presence of immunodominant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichophyton mentagrophytes; Antigens.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300003

Antimycobacterial and cytotoxicity activity of microcystins
Provedor de dados: 80
Autores: Ramos,Daniela Fernandes; Matthiensen,Alexandre; Colvara,Wilson; Votto,Ana Paula Souza de; Trindade,Gilma Santos; Silva,Pedro Eduardo Almeida da; Yunes,João Sarkis.
Background The present work aimed to evaluate the antimycobacterial activity and cytotoxicity of Microcystis aeruginosa toxins, the MC-LR variant and purified extract of [D-Leu1] microcystin-LR. Methods The antimicrobial activity of M. aeruginosa extract and microcystin was evaluated by resazurin microtiter assay against Mycobacterium tuberculosis, M. terrae, M. chelonae and M. kansasii. The cytotoxicity assay was performed by trypan blue exclusion against the HTC cell line. Results Antimicrobial activity was observed in the hexanic extract of M. aeruginosa (RST 9501 strain) against M. tuberculosis, including sensitive and resistant strains with minimal inhibitory concentrations (MIC) between 1.93 μM and 0.06 μM. The high activity of M. aeruginosa hexanic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacteria; Antimycobacterial agents; Cytotoxic activity; Microcystins.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100315

What is the optimum concentration of m-cresol in antivenoms?
Provedor de dados: 80
Autores: Abd-Elsalam,MA; Abdoon,N; Al-Ahaidib,MS.
Antivenoms against snake and scorpion envenomations are usually equine in nature and composed mostly of F(ab')2; additionally, phenol and m-cresol are mainly employed for their preservation. Although there is no study on this subject, m-cresol is utilized by most manufacturers in a concentration that ranges from 0.15 to 0.35 g%. Decreasing the concentration of m-cresol to its minimal effective level may protect victims from its toxic effects and keep the antivenom stable during its shelf life without forming any aggregates. In the present work, different concentrations of m-cresol, ranging from 0.1 to 0.35 g%, were used with some selected batches of snake and scorpion antivenoms. A low concentration of 0.15 g% showed an acceptable preserving result that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antivenoms; Preservative; M-cresol; Stability.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100003

Epidemiology of snakebites in Kédougou region (eastern Senegal): comparison of various methods for assessment of incidence and mortality
Provedor de dados: 80
Autores: Lam,Absa; Camara,Bouna; Kane,Oumar; Diouf,Amadou; Chippaux,Jean-Philippe.
Abstract  Background  Although considered a public health issue in Senegal, the actual incidence and mortality from snakebite are not known. In the present study, an epidemiological survey was carried out in Kédougou region, southeastern Senegal, where envenomations, particularly by Echisocellatus, are frequent and severe.  Methods  Three sources of data were used: records from health centers and reports by health professionals; traditional healers; and household surveys.  Results  The annual incidence and mortality provided by health centers were 24.4 envenomations and 0.24 deaths per 100,000 population, respectively. The annual incidence recorded by traditional healers was 250 bites per 100,000 inhabitants, but the number of deaths was unknown. Finally,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epidemiology; Snakebite; Envenomation; Senegal; Incidence.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100307

Cytotoxic and inflammatory potential of a phospholipase A2 from Bothrops jararaca snake venom
Provedor de dados: 80
Autores: Cedro,Rafhaella C. A.; Menaldo,Danilo L.; Costa,Tássia R.; Zoccal,Karina F.; Sartim,Marco A.; Santos-Filho,Norival A.; Faccioli,Lúcia H.; Sampaio,Suely V.
Abstract  Background: Snake venom phospholipases A2 (PLA2s) have been reported to induce myotoxic, neurotoxic, hemolytic, edematogenic, cytotoxic and proinflammatory effects. This work aimed at the isolation and functional characterization of a PLA2 isolated from Bothrops jararaca venom, named BJ-PLA2-I.  Methods and Results: For its purification, three consecutive chromatographic steps were used (Sephacryl S-200, Source 15Q and Mono Q 5/50 GL). BJ-PLA2-I showed acidic characteristics, with pI~4.4 and molecular mass of 14. 2 kDa. Sequencing resulted in 60 amino acid residues that showed high similarity to other Bothrops PLA2s, including 100% identity with BJ-PLA2, an Asp49 PLA2 previously isolated from B. jararaca venom. Being an Asp49 PLA2, BJ-PLA2-I...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops jararaca; Phospholipase A2; Inflammation; Cytotoxicity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100324

Risk factors caused by scorpion stings and envenomations in the province of Kelâa Des Sraghna (Morocco)
Provedor de dados: 80
Autores: Hmimou,R; Soulaymani,A; Mokhtari,A; Arfaoui,A; Eloufir,G; Semlali,I; Soulaymani Bencheikh,R.
The present study aims to elucidate the risk factors involved in deaths caused by scorpion envenomation. A retrospective study was carried out in the province of Kelâa des Sraghna from 2003 to 2004. The hospital charts of scorpion envenomation cases treated in the Essalama Hospital in Kelâa des Sraghna were exhaustively analyzed. Our study contains 31 cases of death among 470 hospitalization cases. Most stings had occurred during the hot period peaking in July and August (54.6%). Moreover, the scorpion stings occurred at night in 60.1% of the cases, between 6:00 pm and 6:00 am. In addition, all age groups were affected by envenomation, with a higher frequency in children aged less than 15 years (68.3%). The envenomation rate (class II + class III) was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion; Morocco; Epidemiology; Kelâa province of Sraghna.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400006

Integrins, cancer and snake toxins (mini-review)
Provedor de dados: 80
Autores: Rádis-Baptista,G.
Integrins encompass a family of transmembrane heterodimeric proteins of adhesion that maintain cells attached to other cells and to the extracellular matrix (ECM). Integrins work as bi-directional mechanotransducers, conveying mechanical signal from outside to inside the cell through a cascade of phosphorylation signals. On the other hand, the signal from inside to outside controls the strength and affinity of integrin adhesion. As proteins of focal contact, integrins are involved in diverse cell functions, such as cell activation, migration, growth, and survival. In the development of neoplastic disease and metastatic tumor, integrins can influence cancer invasiveness and progression, as well as mediate the formation of new blood vessels (angiogenesis)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Integrin; Angiogenesis; Cancer; Apoptosis; Snake toxin; Snake venom C-type lectin; Metalloprotease; Disintegrin.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300002

Angiotensin-converting enzyme inhibitors of Bothrops jararaca snake venom affect the structure of mice seminiferous epithelium
Provedor de dados: 80
Autores: Alberto-Silva,Carlos; Gilio,Joyce M; Portaro,Fernanda C. V.; Querobino,Samyr M.; Camargo,Antonio C. M.
Background Considering the similarity between the testis-specific isoform of angiotensin-converting enzyme and the C-terminal catalytic domain of somatic ACE as well as the structural and functional variability of its natural inhibitors, known as bradykinin-potentiating peptides (BPPs), the effects of different synthetic peptides, BPP-10c (&lt;ENWPHQIPP), BPP-11e (&lt;EARPPHPPIPP), BPP-AP (&lt;EARPPHPPIPPAP) and captopril were evaluated in the seminiferous epithelium of male mice.Methods The adult animals received either one of the synthetic peptides or captopril (120 nmol/dose per testis) via injection into the testicular parenchyma. After seven days, the mice were sacrificed, and the testes were collected for histopathological evaluation.Results BPP-10c...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: B. jararaca; Bradykinin-potentiating peptides; Angiotensin-converting enzyme; Seminiferous epithelium; Spermatogenesis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100338

Immunohistochemical investigation of neuronal injury in cerebral cortex of cobra-envenomed rats
Provedor de dados: 80
Autores: Rahmy,T.R.; Hassona,I.A.
The immunohistochemical expression of neuron-specific enolase, NSE (a cytoplasmic glycolytic enzyme of the neurons), synaptophysin, SYN (a major membrane glycoprotein of synaptic vesicles), and Bcl-2 (anti-apoptotic protein) were determined in cerebral cortex of rats envenomed with neurotoxic venom from Egyptian cobra. Male rats were intramuscularly (IM) injected with a single injection of either physiological saline solution or ½ LD50 or LD50 of cobra venom and sacrificed 24, 48, or 72 hr after envenoming. Formalin-fixed paraffin sections were immunohistochemically studied by avidin-biotin-peroxidase complex method. Neuron histological structure and isolation of genomic DNA were also detected. The results showed a dose and time-dependent increase in NSE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunohistochemistry; Neuron specific enolase; Synaptophysin; Bcl-2; Cerebral cortex; Cobra snake venom.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000100005

Evaluation of the intestinal microbiota of individuals injured by biological materials in occupational accidents and subjected to antiretroviral prophylaxis
Provedor de dados: 80
Autores: Souza,M. E.
Tipo: Info:eu-repo/semantics/other
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300012

Life quality of people infected by hiv with or without antiretroviral treatment
Provedor de dados: 80
Autores: Gil,N. L. M.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300019

Effect of phospholipase A2 inhibitors during infection caused by Leishmania (Leishmania) amazonensis
Provedor de dados: 80
Autores: Bordon,Maria L. A. C.; Laurenti,Márcia D.; Ribeiro,Susan Pereira; Toyama,Marcos H.; Toyama,Daniela de O.; Passero,Luiz Felipe D.
Abstract  Background: Lipid metabolites play an important role in parasite differentiation and virulence. Studies have revealed that Leishmania sp. uses prostaglandins to evade innate barriers, thus enabling the parasites to survive inside immune cells. Despite the role of the enzyme Phospholipase A2 (PLA2) in prostaglandins production, few studies have investigated the role of parasite PLA2 during the interaction between L. (L.) amazonensis and the host (in vitro and in vivo) immune cells.  Methods: In the present work, the leishmanicidal effect of PLA2 inhibitors, methyl arachidonyl fluorophosphonate (MAFP), bromoenol lactone (BEL) and aristolochic acid (AA) were investigated in vitro (promastigote and intracellular amastigote forms of L. (L.)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania (Leishmania) amazonensis; Macrophages; BALB/c mice; Phospholipase A2; Phospholipase A2inhibitors.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100312

Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming
Provedor de dados: 80
Autores: Ferreira,J.; Bastos,M. F.; Pelizon,A. C.; Peres,C. M.; Cavalcante,D. P.; Sartori,A.
Alternative sources of anti-ophidic serum are being investigated due to the secondary effects associated with types I and II hypersensitivity reactions. In the present study we raised and evaluated the protective effect of an ovine antibothropic serum in a Swiss mice envenoming model. Ovine antiserum was obtained by immunization with seven increasing doses of bothropic venom associated with adjuvants. The neutralizing ability was tested by the lethal activity (2 LD50) neutralization and serum and splenic venom levels after antivenom administration to experimentally envenomed mice. The antiserum effect on local edema was also tested by injection of venom/antivenom mixtures into the mice footpads. Ovine antiserum neutralized lethal activity and also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararaca venom; Ovine antiserum; Venom levels; Edema; ELISA.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300010

Purification and partial characterization of phospholipases A2 from Bothrops asper (barba amarilla) snake venom from Chiriguaná (Cesar, Colombia)
Provedor de dados: 80
Autores: Ramírez-Avila,J.; Quevedo,B. E.; López,E.; Renjifo,J. M.
Components with phospholipase A2 activity were isolated by gel filtration and cationic exchange chromatography from the venom of Bothrops asper snakes from Chiriguaná, Colombia (9°22´N; 73°37´W). Five fractions were obtained by the gel filtration, and PLA2 activity was found in fraction 3 (F3). In the cationic exchange chromatography, F3 showed eight components with PLA2 activity. Six of these components appeared as one band in polyacrylamide gel electrophoresis (SDS-PAGE). Fractions II and VII exhibited an optimal activity at pH 9 and 52ºC. The optimum calcium concentration for fraction II was 48 mM and for fraction VII, 384 mM. Both fractions showed thermal stability. Fraction II was stable at pH values between 2.5 and 9, and fraction VII, between 2.5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phospholipase; Isoenzymes; Snake; Venom; Bothrops asper; Colombia.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300005

Heterologous fibrin sealant derived from snake venom: from bench to bedside – an overview
Provedor de dados: 80
Autores: Ferreira Jr,Rui Seabra; Barros,Luciana Curtolo de; Abbade,Luciana Patrícia Fernandes; Barraviera,Silvia Regina Catharino Sartori; Silvares,Maria Regina Cavariani; Pontes,Leticia Gomes de; Santos,Lucilene Delazari dos; Barraviera,Benedito.
Abstract Hemostatic and adhesive agents date back to World War II, when homologous fibrin sealant came onto scene. Considering that infectious diseases can be transmitted via human blood, a new heterologous fibrin sealant was standardized in the 1990s. Its components were a serine protease (a thrombin-like enzyme) extracted from the venom of Crotalus durissus terrificus snakes and a fibrinogen-rich cryoprecipitate extracted from the blood of Bubalus bubalis buffaloes. This new bioproduct has been used as a coagulant, sealant, adhesive and recently as a candidate scaffold for mesenchymal stem cells and bone and cartilage repair. This review discusses the composition of a new heterologous fibrin sealant, and cites published articles related to its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibrin sealant; Snake venom; Cryoprecipitate coagulum; Thrombin-like enzyme; Buffaloes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100204

Envenoming by Viridovipera stejnegeri snake: a patient with liver cirrhosis presenting disruption of hemostatic balance
Provedor de dados: 80
Autores: Chien,Chih-Ying; Liao,Shu-Chen; Liao,Chien-Hung; Huang,Ting-Shuo; Chen,Yu-Hsien.
Abstract  Background  In most cases of envenoming by the green habu Viridovipera stejnegeri in Taiwan coagulopathy is not observed.  Case presentation  Herein, we describe the case of a patient with liver cirrhosis who developed venom-induced consumptive coagulopathy after V. stejnegeri bite. Laboratory investigation revealed the following: prothrombin time &gt; 100 s (international normalized ratio &gt; 10), activated partial thromboplastin time &gt; 100 s, fibrinogen &lt; 50 mg/dL, and fibrin degradation product &gt; 80 μg/mL. The patient recovered after administration of bivalent hemorrhagic antivenom, vitamin K, fresh frozen plasma and cryoprecipitate.  Conclusion  The liver, directly involved in the acute phase reaction, is the main responsible for...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Snakebite; Liver cirrhosis; Coagulopathy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100501

Experimental use of fibrin glue derived from snake venom in non-pregnant canine uterus
Provedor de dados: 80
Autores: Moraes,J.R.E.; Correia,P.H.A.; Camplesi,A.C.; Moraes,F.R.
This study evaluated the use of fibrin glue derived from snake venom in the healing process after canine hysterorrhaphy. Three groups of four animals were submitted to uterine hysterotomy followed by wound closure. In Group 1, double-layer suture was used, the first with Schimieden pattern, the second with Cushing pattern; in Group 2, only fibrin glue; and in Group 3, the same as for Group 1 but with fibrin glue as suture reinforcement. Results indicated that fibrin glue produced less inflammation in the exudative phase, and exacerbated deposition of connective tissue and angiogenesis in the proliferative and maturation phases of the healing process, favoring its evolution.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Healing; Fibrin glue; Uterus; Surgical adhesives; Wound closure.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200003

American trypanosomiasis and associated risk factors in owned dogs from the major city of Yucatan, Mexico
Provedor de dados: 80
Autores: Jiménez-Coello,Matilde; Acosta-Viana,Karla; Guzmán-Marín,Eugenia; Bárcenas-Irabién,Alejandra; Ortega-Pacheco,Antonio.
AbstractBackground The American trypanosomiasis is a zoonosis caused by the protozoaTrypanosoma cruzi (T. cruzi). The disease is widely distributed throughout the American continent, affecting a wide range of hosts, including dogs. It is present in the canine population in the State of Yucatan, Mexico. However, no significant studies in owned dogs have been performed in the metropolitan area of Merida. A transversal study was conducted in 370 owned dogs from Merida, Yucatan, Mexico.Methods A cross-sectional study including 370 dogs was performed in a major city of Yucatan, Mexico, to detect IgG antibodies against T. cruzi. A commercial ELISA test kit was used and a chi-square test used to evaluate associated risk factors; odds ratio (OR) and 95 %...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Epidemiology; Owned dogs; Major city; Mexico.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100343

A novel Kv1.1 potassium channel blocking toxin from the venom of Palamneus gravimanus (Indian black scorpion)
Provedor de dados: 80
Autores: More,S. S.; Mirajkar,K. K.; Gadag,J. R.; Menon,K. S.; Mathew,M. K.
A peptide toxin was isolated from the venom of Palamneus gravimanus, the Indian black scorpion, to block human Kv1.1 channels expressed in Xenopus laevis oocytes. A 4.5 kD peptide (toxin), as confirmed by SDS-PAGE, was purified to homogeneity by ion exchange chromatography using CM-Sephadex C-25 followed by Sephadex G-50 gel filtration. Palamneus gravimanus toxin (PGT) selectively blocks the human cloned voltage-gated potassium channel hKv1.1 in a two-electrode voltage-clamp (TEVC) technique. The results obtained indicate that the toxin blocks the hKv1.1 channel at a nanomolar concentration range (Ki value of 10 nM) of the peptide to the external side of the cell. The blockage seems to be voltage-dependent. Comparative structure of PGT (a 4.5 kD peptide)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HKv1.1; K+ channel; PGT; TEVC; Palamneus gravimanus; Xenopus laevis oocytes; Indian black scorpion.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300009

Purification of a 19-kDa pore-forming cytolysin from the sea anemone Heteractis magnifica
Provedor de dados: 80
Autores: Karthikayalu,S; Rama,V; Venkatesan,R.
Pore-forming cytolysins of 19 kDa from sea anemones present a remarkable cytolytic property. In the present work, a purified 19-kDa cytolysin was obtained from the sea anemone Heteractis magnifica. The purification steps involved ammonium sulfate precipitation and subsequently desalting by dialysis against 10 mM sodium phosphate buffer (pH 7.4), followed by anion exchange chromatography in DEAE-Sepharose® column (GE Healthcare, Sweden) and gel filtration chromatography using Sephadex® G-50 matrix (GE Healthcare, Sweden). The active fractions from the gel filtration chromatography were pooled and rechromatographed in the same column. The final active fraction showed a prominent protein band of molecular mass of 19 kDa when analyzed by SDS-PAGE.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Heteractis magnifica; Cytolysin; Hemolysin; Pore-forming toxin.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300019

Medically important beetles (insecta: coleoptera) of Iran
Provedor de dados: 80
Autores: Nikbakhtzadeh,MR; Tirgari,S.
This study focused on coleopteran species that are responsible for the emergence of recent cases of dermatological manifestations in Iran. To the best of our knowledge, five species of the family Meloidae and nine species of the genus Paederus are by far the only beetles recognized as medically important in Iran. The staphylinids consists of Paederus ilsae, P. iliensis, P. fuscipes, P. kalalovae, P. balcanicus, P. lenkoranus, P. littoralis, P. carpathicus, P. nigricornis, while the meloids are Mylabris impressa, M. guerini, Muzimes iranicus, Alosimus smyrnensis and Epicauta sharpi. Most cases of linear dermatitis in this country occur in areas bordering the Caspian Sea. This problem is caused by beetles of the genus Paederus which are present as adults...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meloidae; Staphylinidae; Paederus; Beetle; Dermatitis; Pederin; Cantharidin; Iran.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400004

Domestic feline cutaneous leishmaniasis in the municipality of Ribas do Rio Pardo, Mato Grosso do Sul state, Brazil: a case report
Provedor de dados: 80
Autores: Souza,AI; Nunes,VLB; Borralho,VM; Ishikawa,EAY.
Cutaneous leishmaniases are anthropozoonoses that involve many species of Leishmania and a wide variety of wild mammalian hosts, thus presenting high importance to public health. This study reports the second case of feline leishmaniasis in Mato Grosso do Sul state, in which Leishmania (Leishmania) amazonensis was found in a domestic cat from Ribas do Rio Pardo. Clinical signs were similar to those observed in other diseases commonly diagnosed in cats, such as cryptococcosis and sporotrichosis. Cutaneous leishmaniasis should, therefore, be added to differential diagnoses by feline veterinary practitioners, and also adequate investigations should be carried out to verify the relevance of domestic cats as L. amazonensis reservoirs.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cutaneous leishmaniasis; Leishmania (Leishmania) amazonensis; Domestic cat; Mato Grosso do Sul state.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200017

Production of prostaglandins and leukotrienes by Paracoccidioides brasiliensis
Provedor de dados: 80
Autores: Biondo,G. A.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100017

Composition and biological activities of the aqueous extracts of three scleractinian corals from the Mexican Caribbean: Pseudodiploria strigosa, Porites astreoides and Siderastrea siderea
Provedor de dados: 80
Autores: García-Arredondo,Alejandro; Rojas-Molina,Alejandra; Ibarra-Alvarado,César; Lazcano-Pérez,Fernando; Arreguín-Espinosa,Roberto; Sánchez-Rodríguez,Judith.
Abstract  Background: Scleractinian corals (stony corals) are the most abundant reef-forming cnidarians found in coral reefs throughout the world. Despite their abundance and ecological importance, information about the diversity of their toxins and their biological activities is very scarce. In this study, the chemical composition and the biological activities of the aqueous extracts of Pseudodiploria strigosa, Porites astreoides and Siderastrea siderea, three scleractinian corals from the Mexican Caribbean, have been assessed for the first time.  Methods: Toxicity of the extracts was assessed in crickets; the presence of cytolysins was detected by the hemolysis assay; the vasoconstrictor activity was determined by the isolated rat aortic ring assay; the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scleractinia; Pseudodiploria strigosa; Porites astreoides; Siderastrea siderea; Toxicity; Hemolysis; Vasoconstriction; Nociceptive response.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100323

Risk of peritonitis during peritoneal dialysis in carriers of Staphylococcus aureus and coagulase-negative staphylococci
Provedor de dados: 80
Autores: Batalha,J. E. N.; Caramori,J. C. T.; Corrente,J. E.; Montelli,A. C.; Barretti,P.; Cunha,M. L. R. S.
The presence of Staphylococcus aureus in the nasal cavities and pericatheter skin of peritoneal dialysis patients put them at high risk of developing peritonitis. However, it is not clear whether the presence of coagulase-negative staphylococci (CNS) in the nasal passages and skin of patients is related to subsequent occurrence of peritoneal infection. The aim of the present study was to verify the relationship between endogenous sources of S. aureus and CNS and occurrence of peritonitis in patients undergoing peritoneal dialysis. Thirty-two patients on peritoneal hemodialysis were observed for 18 months. Staphylococcus species present in their nasal passage, pericatheter skin and peritoneal effluent were identified and compared based on drug...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Coagulase-negative staphylococci; Carriers; Peritoneal dialysis; Peritonitis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400005

Functional and biological insights of rCollinein-1, a recombinant serine protease from Crotalus durissus collilineatus
Provedor de dados: 80
Autores: Boldrini-França,Johara; Pinheiro-Junior,Ernesto Lopes; Arantes,Eliane Candiani.
ABSTRACT  Background: The prevalent class of snake venom serine proteases (SVSP) in Viperidae venoms is the thrombin-like enzymes, which, similarly to human thrombin, convert fibrinogen into insoluble fibrin monomers. However, thrombin-like serine proteases differ from thrombin by being unable to activate factor XIII, thus leading to the formation of loose clots and fibrinogen consumption. We report the functional and biological characterization of a recombinant thrombin-like serine protease from Crotalus durissus collilineatus, named rCollinein-1.  Methods: Heterologous expression of rCollinein-1 was performed in Pichia pastoris system according to a previously standardized protocol, with some modifications. rCollinein-1 was purified from the culture...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Serine proteases; Thrombin-like enzymes; Coagulation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100306

Antivenom reversal of biochemical alterations induced by black scorpion Heterometrus fastigiousus Couzijn venom in mice
Provedor de dados: 80
Autores: Chaubey,MK.
In the present study, Heterometrus fastigiousus venom (HFV) was employed as antigen to produce species-specific scorpion antivenom (SAV) in albino mice (NIH) strain. To determine SAV efficacy, it was pre-incubated with 10 LD50 of HFV and then injected subcutaneously into mice. Subsequently, mortality was observed after 24 hours. Minimum effective dose (MED) was 12.5 LD50 of HFV/mL of SAV. SAV effectiveness to reverse HFV-induced biochemical alterations in mice was analyzed by challenge method. Simultaneously, mice received subcutaneously 40% of 24-hour-LD50 of HFV and intravenously SAV. After four hours, changes in serum glucose, free amino acids, uric acids, pyruvic acid, cholesterol, total protein, alkaline phosphatase, acid phosphatase, lactic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venom; Antivenom; Envenomation; Heterometrus fastigiousus; Serotherapy.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200006

Tropical House Gecko (Hemidactylus mabouia) predation on brown spiders (Loxosceles intermedia)
Provedor de dados: 80
Autores: Ramires,E.N.; Fraguas,G.M.
Brown spiders (Loxosceles spp.) are venomous arachnids, successfully adapted to urban habitats in Brazil. Loxoscelism became a serious public health problem in Paraná State, especially at the capital Curitiba, where the most abundant species is Loxosceles intermedia. Hemidactylus mabouia (Gekkonidae) lizards are synanthropic predators of arthropods. In this paper, we describe the predatory behavior of the Tropical House Gecko H. mabouia on L. intermedia under laboratory conditions. Twelve geckos were observed, and all of them fed on brown spiders (n=123 observations). The attack consisted of a fast run followed by one bite on the spider’s abdomen or legs. The geckos did not attack L. intermedia anterior body parts, probably due to the fangs present in this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemidactylus mabouia; Predatory behavior; Loxosceles intermedia; Sicariidae; Araneism; Gekkonidae; Biological control.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200008

Cytotoxic activity of Androctonus australis hector venom and its toxic fractions on human lung cancer cell line
Provedor de dados: 80
Autores: Béchohra,Louisa; Laraba-Djebari,Fatima; Hammoudi-Triki,Djelila.
Abstract  Background: Several studies have showed that animal venoms are a source of bioactive compounds that may inhibit the growth of cancer cells, which makes them useful agents for therapeutic applications. Recently, it was established that venom toxins from scorpions induced cytotoxic, antiproliferative and apoptogenic effects on cancer cells. Therefore, the present study aims to investigate the cytotoxic activity of Androctonus australis hector (Aah) scorpion venom and its toxic fractions (FtoxG-50 and F3) on NCI-H358 human lung cancer cells.  Methods: The cytotoxic and antiproliferative activities were estimated using MTT assay, lactate dehydrogenase release and clonogenic assays. Apoptosis was evaluated by Hoechst 33258 staining, DNA fragmentation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aah venom; Cytotoxicity; F3 fraction; Apoptosis; Lung cancer cells; Oxidative stress.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100322

Fatal neurotoxic envenomation following the bite of a greater black krait (Bungarus niger) in Nepal: a case report
Provedor de dados: 80
Autores: Pandey,Deb Prasad; Sharma,Sanjib Kumar; Alirol,Emilie; Chappuis,François; Kuch,Ulrich.
Abstract  Background  Neurotoxic envenomation following bites by kraits (Bungarus species) is a leading cause of snakebite mortality in South Asia. Over a long time, this had been attributed only to one species, the common krait (Bungarus caeruleus). However, recent research has provided increasing evidence of the involvement of several krait species. Here, we report a fatal case of neurotoxic envenomation following the bite of a greater black krait (Bungarus niger) in Nepal.  Case presentation  A 33-year-old man was bitten in the outdoor corridor of his home in the eastern hills of Ilam district while handling a snake he thought to be non-venomous. He subsequently developed severe abdominal pain, frequent vomiting, and signs of neurotoxic envenomation...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Bungarus niger; Neurotoxicity; Krait; Envenomation; Antivenom; Snakebite.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100503

The modular nature of bradykinin-potentiating peptides isolated from snake venoms
Provedor de dados: 80
Autores: Sciani,Juliana Mozer; Pimenta,Daniel Carvalho.
Abstract Bradykinin-potentiating peptides (BPPs) are molecules discovered by Sergio Ferreira – who found them in the venom of Bothrops jararaca in the 1960s – that literally potentiate the action of bradykinin in vivo by, allegedly, inhibiting the angiotensin-converting enzymes. After administration, the global physiological effect of BPP is the decrease of the blood pressure. Due to this interesting effect, one of these peptides was used by David Cushman and Miguel Ondetti to develop a hypotensive drug, the widely known captopril, vastly employed on hypertension treatment. From that time on, many studies on BPPs have been conducted, basically describing new peptides and assaying their pharmacological effects, mostly in comparison to captopryl. After...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararaca; Venom; Snake venom; Bradykinin-potentiating peptides; BPP; Modules.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100208

Biomedical and pharmacological potential of tetrodotoxin-producing bacteria isolated from marine pufferfish Arothron hispidus (Muller, 1841)
Provedor de dados: 80
Autores: Bragadeeswaran,S; Therasa,D; Prabhu,K; Kathiresan,K.
Specimens of the pufferfish Arothron hispidus collected at Parangipettai, on the southeast coast of India, were subjected to bacterial isolation and identification. Three species were identified, namely Bacillus sp., Kytococcus sedentarius and Cellulomonas fimi. Partially-purified microbial filtrates exhibited hemolytic activity on chicken and human erythrocytes of O, B and AB blood groups, with maximum activity of 32 HU. The microbial filtrates also presented ATPase, Mg2+-ATPase, Na+K+-ATPase and AchE enzymatic activities of positive neuromodulation in Kytococcus sedentarius with 1300, 300.1, 1549.98 and 140.55%, in Cellulomonas fimi with 620, 300, 10 and 128.42%, and in Bacillus species with 40, 200, 849.98 and 158.69%, respectively. Toxicity symptoms...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arothron hispidus; Tetrodotoxin; Bioassay; ATPase and AchE enzymatic activities; Anticancer activity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300008

Renal failure and neonatal death following snakebite during pregnancy: a case report
Provedor de dados: 80
Autores: Adam,G. K.; Hussein,S. M.; Adam,I.
A thirty-five-year-old Sudanese woman (gravida 4, para 3), at 34 weeks gestational age, presented with snakebite in her right leg. Examination of the cardiovascular, respiratory, gastrointestinal, and central nervous systems revealed no abnormalities. Her blood urea was 58 mg/dl; creatinine, 2.6 mg/dl; whole blood clotting time, 5:35 minutes. The patient was diagnosed as having acute renal failure and was then managed conservatively. She received polyvalent antivenom serum intravenously. On the next day, she delivered prematurely and the baby passed away due to respiratory distress. There was no postpartum bleeding and the patient's clotting time was 5:30 minutes. She was discharged from the hospital after 7 days when her clinical, biochemical and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake; Renal; Pregnancy; Perinatal; Sudan.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400011

Fine structural analysis of the stinger in venom apparatus of the scorpion Euscorpius mingrelicus (Scorpiones: Euscorpiidae)
Provedor de dados: 80
Autores: Yigit,N; Benli,M.
In this study, the morphology, histology and fine structure of the stinger, a part of the venom apparatus of Euscorpius mingrelicus (Kessler, 1874) (Scorpiones: Euscorpiidae) were studied by light microscopy and transmission electron microscopy (TEM). The stinger, located at the end section of the telson, is sickle-shaped. The venom is ejected through a pair of venom pores on its subterminal portion. Both venom ducts extend along the stinger without contact with each other since they are separated by connective tissue cells. The stinger cuticle is composed of two layers. Additionally, there are many pore canals and some hemolymph vessels in the cuticle. This work constitutes the first histological and fine structure study on Euscorpius mingrelicus stinger.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion; Euscorpius mingrelicus; Telson; Stinger; Fine structure; Transmission electron microscopy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100008

Study of irradiated bothropstoxin-1 with60Co gamma rays: immune system behavior
Provedor de dados: 80
Autores: Caproni,P; Baptista,JA; Almeida,TL de; Passos,LAC; Nascimento,N.
Ionizing radiation has been successfully employed to modify the immunological properties of biomolecules. Very promising results were obtained when crude animal venoms, as well as isolated toxins, were treated with 60Co gamma rays, yielding toxoids with good immunogenicity. The achievement of modified antigens with lower toxicity and preserved or improved immunogenicity can be very useful. Ionizing radiation has already been proven to be a powerful tool to attenuate snake venom toxicity without affecting, and even increasing, their immunogenic properties. However, little is known about the modifications that irradiated molecules undergo and even less about the immunological response that such antigens elicit. In the present work, we investigated the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunology; Ionizing radiation; Gamma rays; Bothropstoxin-1.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200005

Toxicity of puffer fish: two species (Lagocephalus laevigatus, linaeus 1766 and Sphoeroides spengleri, Bloch 1785) from the Southeastern Brazilian coast
Provedor de dados: 80
Autores: Oliveira,J. S.; Pires Junior,O. R.; Morales,R. A. V.; Bloch Junior,C.; Schwartz,C. A.; Freitas,J. C.
In Brazil, where puffer fish are considered poisonous, there are few documented cases on human consumption and consequent poisoning. In this study, toxicity of two puffer fish species from the Brazilian coast was examined. Specimens of Sphoeroides spengleri and Lagocephalus laevigatus were caught in São Sebastião Channel (North coast of São Paulo State, Brazil) between January 1996 and May 1997. Acidic ethanol extracts from muscle and skin plus viscera were tested for mice acute toxicity using the standard method of Kawabata. Polar extracts of S. spengleri showed high toxicity up to 946 MU/g. Extracts from L. laevigatus showed very low levels of toxicity, never exceeding 1.7 MU/g. All extracts from both species blocked amielinic nerve fiber evoked impulses...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Puffer fish; Brazilian puffer; Tetrodotoxin (TTX); Envenoming; HPLC and mass spectrometry.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100005

BaltDC: purification, characterization and infrared spectroscopy of an antiplatelet DC protein isolated from Bothrops alternatus snake venom
Provedor de dados: 80
Autores: Matias,Mariana Santos; Sousa,Bruna Barbosa de; Pereira,Déborah Fernanda da Cunha; Dias,Edigar Henrique Vaz; Mamede,Carla Cristine Neves; Queiroz,Mayara Ribeiro de; Silva,Anielle Christine Almeida; Dantas,Noelio Oliveira; Soares,Andreimar Martins; Costa,Júnia de Oliveira; Oliveira,Fábio de.
Abstract  Background: Snake venoms are a complex mixture of proteins, organic and inorganic compounds. Some of these proteins, enzymatic or non-enzymatic ones, are able to interact with platelet receptors, causing hemostatic disorders. The possible therapeutic potential of toxins with antiplatelet properties may arouse interest in the pharmacological areas. The present study aimed to purify and characterize an antiplatelet DC protein from Bothrops alternatus snake venom.  Methods: The protein, called BaltDC (DC protein from B. alternatus snake venom), was purified by a combination of ion-exchange chromatography on DEAE-Sephacel column and gel filtration on Sephadex G-75. The molecular mass was estimated by polyacrylamide gel electrophoresis in the presence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops alternatus; DC protein; Platelet aggregation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100324

Infestation by Aedes albopictus (skuse) in natural and artificial breeding sites found in green areas in the city of Fortaleza, Ceará
Provedor de dados: 80
Autores: Alencar,C. H. M.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300018

Mass fingerprinting and electrophysiological analysis of the venom from the scorpion Centruroides hirsutipalpus (Scorpiones: Buthidae)
Provedor de dados: 80
Autores: Valdez-Velázquez,Laura L.; Olamendi-Portugal,Timoteo; Restano-Cassulini,Rita; Zamudio,Fernando Z.; Possani,Lourival D.
Abstract  Background Centruroides hirsutipalpus, of the family Buthidae, is a scorpion endemic to the Western Pacific region of Mexico. Although medically important, its venom has not yet been studied. Therefore, this communication aims to identify their venom components and possible functions.  Methods  Fingerprinting mass analysis of the soluble venom from this scorpion was achieved by high-performance liquid chromatography and electrospray mass spectrometry. Furthermore, the soluble venom and its toxic effects were evaluated extensively via electrophysiological assays in HEK cells expressing human voltage-gated Na+ channels (hNav 1.1 to Nav1.6), CHO cells expressing hNav 1.7, potassium channel hERG 1 (Ether-à-go-go-related-gene) and the human K+-channel...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Centruroides; Electrophysiology; Mass fingerprinting; Scorpion venom.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100310

Highlights in the knowledge of brown spider toxins
Provedor de dados: 80
Autores: Chaves-Moreira,Daniele; Senff-Ribeiro,Andrea; Wille,Ana Carolina Martins; Gremski,Luiza Helena; Chaim,Olga Meiri; Veiga,Silvio Sanches.
Abstract Brown spiders are venomous arthropods that use their venom for predation and defense. In humans, bites of these animals provoke injuries including dermonecrosis with gravitational spread of lesions, hematological abnormalities and impaired renal function. The signs and symptoms observed following a brown spider bite are called loxoscelism. Brown spider venom is a complex mixture of toxins enriched in low molecular mass proteins (4–40 kDa). Characterization of the venom confirmed the presence of three highly expressed protein classes: phospholipases D, metalloproteases (astacins) and insecticidal peptides (knottins). Recently, toxins with low levels of expression have also been found in Loxosceles venom, such as serine proteases, protease...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brown spider; Loxosceles; Venom; Toxins; Loxoscelism; Phospholipase-D; Metalloprotease; Insecticidal peptides; Serineprotease; Hyaluronidase.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100201

October 10th: worldwide Open Access Day
Provedor de dados: 80
Autores: Kuramoto,Hélio.
Tipo: Info:eu-repo/semantics/other
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400001

PP65 antigenemia in the diagnosis of cytomegalovirus infection in AIDS patients
Provedor de dados: 80
Autores: Capela,RC; Grassi,A; Souza,LR.
Cytomegalovirus causes significant morbidity and mortality in AIDS patients and those having undergone bone marrow or another transplant. PP65 antigenemia is based on detecting viral antigen in peripheral blood leukocytes through immunochemistry and by monitoring the infection in immunocompromised individuals. The present study aimed to set up this diagnostic technique in AIDS patients with active cytomegalovirus infection and verify its occurrence in the Botucatu region of São Paulo state, Brazil. Fifty patients, 35 men and 15 women aged from 24 to 69 years, were recruited from those attended at the Department of Tropical Diseases of Botucatu Medical School, UNESP, and divided into three groups according to CD4+ T lymphocyte counts and antiretroviral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acquired immunodeficiency syndrome; Cytomegalovirus; Pp65 antigen; Laboratory diagnosis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100013

Restoration of adenosine deaminase, histamine, and IgE in organs of mice injected with cobra venom followed by specific treatment and reversal period
Provedor de dados: 80
Autores: Lipps,B. V.
Adenosine deaminase (ADA), histamine, and IgE are endogenously present in animals. Research from this laboratory reported decreased levels of these substances in organs of mice as a consequence of sub-lethal injection of Naja kaouthia venom. This research reports that decreased ADA, histamine, and IgE levels were prevented by specific treatment and prolonged recovery periods. Adult Balb/c mice injected IM with sub-lethal venom dose were divided into five groups. Group 1 were injected with PBS; Group 2 with anti-cobra venom; and Group 3 with lethal toxin neutralizing factor (LTNF). Groups 4 and 5 were treated with IM or oral synthetic LT-10. After 24 hours, mice were sacrificed and organ homogenates were assayed for ADA, histamine, and IgE. Group 1 showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cobra venom; Mouse organs; ADA; Histamine; IgE; Homeostasis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200007

Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis
Provedor de dados: 80
Autores: Diniz-Sousa,Rafaela; Kayano,Anderson M.; Caldeira,Cleópatra A.; Simões-Silva,Rodrigo; Monteiro,Marta C.; Moreira-Dill,Leandro S.; Grabner,Fernando P.; Calderon,Leonardo A.; Zuliani,Juliana P.; Stábeli,Rodrigo G.; Soares,Andreimar M.
Abstract  Background: Wasp venoms constitute a molecular reservoir of new pharmacological substances such as peptides and proteins, biological property holders, many of which are yet to be identified. Exploring these sources may lead to the discovery of molecules hitherto unknown. This study describes, for the first time in hymenopteran venoms, the identification of an enzymatically inactive phospholipase A2 (PLA2) from the venom of the social wasp Polybia occidentalis.  Methods: P. occidentalis venom was fractioned by molecular exclusion and reverse phase chromatography. For the biochemical characterization of the protein, 1D and 2D SDS-PAGE were performed, along with phospholipase activity assays on synthetic substrates, MALDI-TOF mass spectrometry and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wasp; Polybia occidentalis; PocTX; PLA2 homologue.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100302

Levels of interleukins 12 (IL-12) and 13 (IL-13), hepatitis B and C serology, and blood cultures among acute myeloid leukemia (AML) patients in Egypt
Provedor de dados: 80
Autores: RAA,Nour el deen; RA,Harfoush; MM,Elgharabawy; NA,Hamed; MG,Morsi.
There is an interest in the use of IL-12 as a possible anti-cancer drug to induce immune responses and anti-IL-13 formulations to treat the undesirable effects of IL-13. Thus, the present study aimed at analyzing IL-12 and IL-13 profiles, viral hepatitis serology and blood cultures in acute myeloid leukemia (AML) patients. Forty individuals (20 without septicemia - Group A, and 20 with septicemia - Group B) and 20 healthy controls were evaluated. Hepatitis B virus antigens (HBsAg) and hepatitis C virus antibodies (HCV Ab) were quantified using commercial ELISA kits. IL-12 and IL-13 levels were estimated in culture supernatant of mitogen-stimulated peripheral blood mononuclear cells by ELISA. Significantly low IL-12 values were observed among AML patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute myeloid leukemia; IL-12; IL-13; HBsAg; HCV; ELISA.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300009

Study on the susceptibility of Enterococcus faecalis from infectious processes to ciprofloxacin and vancomycin
Provedor de dados: 80
Autores: Genaro,A.; Cunha,M. L. R. S.; Lopes,C. A. M.
Enterococcus faecalis is considered a pathogen responsible for hospital infections and, due to its frequent multi-resistant profile, has caused preoccupations among many medical authorities. The objective of this study was to determine the antimicrobial susceptibility of 74 strains isolated from blood cultures and purulent secretions to vancomycin and ciprofloxacin through the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) by using the Microdilution test. The results showed a greater efficacy of vancomycin compared to ciprofloxacin (98.6% of the strains were inhibited by vancomycin at lower concentrations: 0.06 - 1 µg/ml). However, in the MBC analysis 73% of the strains showed a MBC of vancomycin only at high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterococcus faecalis; Vancomycin; Ciprofloxacin; Resistance; Spontaneous mutation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300004

Young ovine death during hyperimmunization: crotalic envenomation or copper toxicosis?
Provedor de dados: 80
Autores: Ferreira Junior,RS; Nascimento,N; Couto,R; Alves,JB; Meira,DA; Barraviera,B.
The unfavorable evolution of a young ovine during hyperimmunization process with Crotalus durissus terrificus venom was investigated in order to differentiate its origin between ophidic envenomation and copper toxicosis. Clinical, laboratory, necroscopic and histological exams as well as evaluation and measurement of heavy metals (copper) in the kidneys and in the liver were carried out. Blood counts revealed anemia and serological tests showed high levels of blood urea nitrogen, creatinine, aspartate aminotransferase, creatine phosphokinase, total bilirubin and indirect bilirubin; which indicates liver, kidney and skeletal muscle damages. At necropsy, the animal presented hepatopathy and nephropathy. Histological examination revealed renal and hepatic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Differential diagnosis; Copper toxicosis; Ovines; Crotalic venom; Hyperimmunization.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400015

Antibody and cytokine serum levels in patients subjected to anti-rabies prophylaxis with serum-vaccination
Provedor de dados: 80
Autores: Ayres,J. A.; Barraviera,B.; Calvi,S. A.; Carvalho,N. R.; Peraçoli,M. T. S.
Rabies is considered a fatal disease once clinical symptoms have developed. The aim of this study was to evaluate epidemiological aspects and immune response in patients attacked by domestic and wild animals and subjected to post-exposure rabies treatment with equine serum and associated vaccine. Thirty-three patients were evaluated; they were between 13 and 65 years old, 75.8% were male and 24.2% female, and from the Botucatu neighborhood. Twenty healthy control individuals with the same age range were also studied. Specific antibodies to equine immunoglobulins and IFN-gamma, IL-2, IL-4, and IL-10 production were evaluated by ELISA. IgM, IgE, IgG and subclasses, and rabies virus antibodies serum levels were determined by nephelometry and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Anti-rabies prophylaxis; Immunoglobulins; Cytokines.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300008

Consecutive bites on two persons by the same cobra: a case report
Provedor de dados: 80
Autores: Amin,MR; Mamun,SMH; Chowdhury,NH; Rahman,M; Ghose,A; Al Hasan,A; Faiz,MA.
In tropical countries like Bangladesh, persons are bitten by snakes every day and a considerable number of patients die en route to the hospital. An event of consecutive neurotoxic bites on two men by a single snake was observed in the Snake Bite Study Clinic (SBSC) of the Chittagong Medical College Hospital (CMCH). Two brothers, working in their semi-pucca restaurant, were successively bitten by the same cobra on their lower limbs. Within an hour, they were taken to the CMCH. Few minutes after admission, both developed symptoms of neurotoxicity: ptosis, nasal voice, dysphagia, broken neck sign, etc. They received polyvalent antivenom (Haffkine Bio-Pharmaceuticals Company, India) and other auxiliary treatment immediately. Within few hours, neurotoxic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Consecutive bites; Neurotoxicity; Cobra; Snakebite; Antivenom.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400014

Pharmacokinetics of neutron-irradiated meglumine antimoniate in Leishmania amazonensis-infected BALB/c mice
Provedor de dados: 80
Autores: Borborema,Samanta Etel Treiger; Osso Junior,João Alberto; Andrade Junior,Heitor Franco de; Nascimento,Nanci do.
Abstract  Background:  Cutaneous leishmaniasis (CL) is a parasitic disease caused by the protozoan Leishmania spp. Pentavalent antimonial agents have been used as an effective therapy, despite their side effects and resistant cases. Their pharmacokinetics remain largely unexplored. This study aimed to investigate the pharmacokinetic profile of meglumine antimoniate in a murine model of cutaneous leishmaniasis using a radiotracer approach.  Methods:  Meglumine antimoniate was neutron-irradiated inside a nuclear reactor and was administered once intraperitoneally to uninfected and L. amazonensis-infected BALB/c mice. Different organs and tissues were collected and the total antimony was measured.  Results:  Higher antimony levels were found in infected than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cutaneous leishmaniasis; Meglumine antimoniate; Pharmacokinetics; Biodistribution; Antimony; Radioisotope.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100304

In vitroantibacterial effect of wasp (Vespa orientalis) venom
Provedor de dados: 80
Autores: Jalaei,Jafar; Fazeli,Mehdi; Rajaian,Hamid; Shekarforoush,Seyed Shahram.
Background The emergence of antibacterial resistance against several classes of antibiotics is an inevitable consequence of drug overuse. As antimicrobial resistance spreads throughout the globe, new substances will always be necessary to fight against multidrug-resistant microorganisms. Venoms of many animals have recently gained attention in the search for new antimicrobials to treat infectious diseases. Thefore, the present study aimed to study the antibacterial effects of wasp (Vespa orientalis) crude venom. Two gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two gram-negative ones (Escherichia coli and Klesiella pneumonia) were compared for their sensitivity to the venom by determining the inhibition zone (Kirby-Bauer method)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wasp venom; Vespa orientalis; Antimicrobial activity; Minimum inhibitory concentration.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200329

Oral Candida spp. colonization in human immunodeficiency virus-infected individuals
Provedor de dados: 80
Autores: Moris,D. V.; Melhem,M. S. C.; Martins,M. A.; Mendes,R. P.
Several yeast species of Candida genus can colonize the skin as well as the mucous membrane of the vagina and the digestive tract for short or long periods. Depending on the host's immunological state and the yeast's virulence, colonization can become an infection, invading the colonized tissues and also disseminating. AIDS is characterized by the host's intensive and progressive immunodepression which manifests as diverse symptoms, mainly lesions in the mouth. Oral candidiasis is the most prevalent opportunistic infection in individuals infected with human immunodeficiency virus (HIV) and is an important indicator of the disease progress and the immunosuppression increase. The factors involved in the equilibrium between Candida spp. and HIV-infected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida spp.; Colonization; Human immunodeficiency virus-infected individuals.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200004

Strategies in ‘snake venomics’ aiming at an integrative view of compositional, functional, and immunological characteristics of venoms
Provedor de dados: 80
Autores: Lomonte,Bruno; Calvete,Juan J.
Abstract This work offers a general overview on the evolving strategies for the proteomic analysis of snake venoms, and discusses how these may be combined through diverse experimental approaches with the goal of achieving a more comprehensive knowledge on the compositional, toxic, and immunological characteristics of venoms. Some recent developments in this field are summarized, highlighting how strategies have evolved from the mere cataloguing of venom components (proteomics/venomics), to a broader exploration of their immunological (antivenomics) and functional (toxicovenomics) characteristics. Altogether, the combination of these complementary strategies is helping to build a wider, more integrative view of the life-threatening protein cocktails...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venoms; Proteomics; Venomics; Antivenomics; Toxicovenomics.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100206

Anaphylatic reaction after Crotalus envenomation treatment in a dog: case report
Provedor de dados: 80
Autores: Conceição,L. G.; Argôlo Neto,N. M.; Castro,A. P.; Faria,L. B. A.; Fonterrada,C. O.
Crotalus envenomation is the second most frequent condition caused by venomous snakes attacking dogs and cats in Brazil. Affected animals show severe neurological and muscular involvement, lower motor neuron signs and myalgia due to extensive rabdomiolysis. The present case report is about Crotalus envenomation in a dog which showed severe clinical and laboratory abnormalities. After treatment with antivenom (specific antidote) and clinical support, the dog totally recovered from that condition.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Crotalus; Dogs; Snake bites; Therapeutics; Anaphylaxis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200012

Destruction of the collection of reptiles and arthropods at Butantan Institute: a view from the United Kingdom
Provedor de dados: 80
Autores: Warrell,DA; Theakston,RDG; Wüster,W.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400003

Rattlesnake Crotalus molossus nigrescens venom induces oxidative stress on human erythrocytes
Provedor de dados: 80
Autores: Meléndez-Martínez,David; Muñoz,Juan Manuel; Barraza-Garza,Guillermo; Cruz-Peréz,Martha Sandra; Gatica-Colima,Ana; Alvarez-Parrilla,Emilio; Plenge-Tellechea,Luis Fernando.
Abstract  Background  Globally, snake envenomation is a well-known cause of death and morbidity. In many cases of snakebite, myonecrosis, dermonecrosis, hemorrhage and neurotoxicity are present. Some of these symptoms may be provoked by the envenomation itself, but others are secondary effects of the produced oxidative stress that enhances the damage produced by the venom toxins. The only oxidative stress effect known in blood is the change in oxidation number of Fe (from ferrous to ferric) in hemoglobin, generating methemoglobin but not in other macromolecules. Currently, the effects of the overproduction of methemoglobin derived from snake venom are not extensively recorded. Therefore, the present study aims to describe the oxidative stress induced by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Attenuated total reflectance-Fourier transform infrared spectroscopy; Crotalus molossus nigrescens; Venom; Snake venom; Methemoglobin; Oxidative stress; Oxyhemoglobin.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100311

Actiflagelin, a new sperm activator isolated from Walterinnesia aegyptia venom using phenotypic screening
Provedor de dados: 80
Autores: El-Aziz,Tarek Mohamed Abd; Khoury,Sawsan Al; Jaquillard,Lucie; Triquigneaux,Mathilde; Martinez,Guillaume; Bourgoin-Voillard,Sandrine; Sève,Michel; Arnoult,Christophe; Beroud,Rémy; Waard,Michel De.
Abstract  Background Sperm contains a wealth of cell surface receptors and ion channels that are required for most of its basic functions such as motility and acrosome reaction. Conversely, animal venoms are enriched in bioactive compounds that primarily target those ion channels and cell surface receptors. We hypothesized, therefore, that animal venoms should be rich enough in sperm-modulating compounds for a drug discovery program. Our objective was to demonstrate this fact by using a sperm-based phenotypic screening to identify positive modulators from the venom of Walterinnesia aegyptia.  Methods Herein, as proof of concept that venoms contain interesting compounds for sperm physiology, we fractionated Walterinnesia aegyptia snake venom by RP-HPLC and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Walterinnesia aegyptia; Bioactive compounds; Fertility; Sperm motility; Venomics; Tandem mass spectrometry; De novo sequencing; Edman degradation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100301

Standardization of an antihemorrhagic potency test of antivenoms prepared from two different Agkistrodon halys venoms
Provedor de dados: 80
Autores: Lee,K. H.; Hur,S. J.; Kim,S. N.; Yoo,S. H.; Shin,I. S.; Won,H. J.; Shin,K. H.; Hong,S. H.; Lee,S. H.; Min,H. K.; Park,S. N.
To establish Korea National Standards for venoms and antivenoms, it is necessary to have standardized assay methods. In this study, we standardized a method to evaluate the antihemorrhagic potency of two horse-derived antivenoms using rabbit intracutaneous injection. We expressed the capability of these antivenoms to neutralize the hemorrhagic activities triggered by the venoms of Agkistrodon halys from Japan and Jiangzhe Agkistrodon halys from China as Minimum Hemorrhagic Dose (MHD). We also performed cross-neutralization tests employing the parallel line assay on different pairings of venoms and antivenoms to check the possibility of using Jiangzhe Agkistrodon halys venom as a substitute for the standard Agkistrodon halys venom in measurements of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antihemorrhagic potency; Minimum hemorrhagic dose; Jiangzhe Agkistrodon halys; Parallel line assay.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300009

Professor José Roberto Giglio and toxinnology in Brazil: 48 years in research
Provedor de dados: 80
Autores: Soares,A. M.; Oliveira,C. Z.; Santana,C. D.; Menaldo,D. L.; Silveira,L. B.; Teixeira,S. S.; Rueda,A. Q.; Marcussi,S.
This work succinctly describes the professional and scientific life of Dr. José R. Giglio, one of the most outstanding Brazilian researchers in the field of Toxinology. During his long and successful career, he has made major contributions, especially in elucidating the function, structure, and mechanisms of action of animal venom proteins (from snakes, scorpions and spiders) as well as the characterization of antibodies and several inhibitors of venoms and toxins. We present here a brief history of Dr. Giglio’s personal and professional life, also reporting some of his numerous published scientific articles on venoms from snakes (Bothrops, Crotalus, and other genera), scorpions (Tityus sp), spiders (Phoneutria sp), their isolated toxins and natural...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxinology; Animal venoms; Toxins; Inhibitors; Homage; Giglio JR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400003

Antivenomic approach of different Crotalus durissus collilineatus venoms
Provedor de dados: 80
Autores: Oliveira,Isadora Sousa de; Pucca,Manuela Berto; Sampaio,Suely Vilela; Arantes,Eliane Candiani.
Abstract  Background: Our group has previously performed a proteomic study verifying that individual variations can occur among Crotalus durissus collilineatus venoms. These variations may lead to differences in venom toxicity and may result in lack of neutralization of some components by antivenom. In this way, this study aimed to evaluate the Brazilian anticrotalic serum capacity in recognizing twenty-two Crotalus durissus collilineatus venoms, as well as their fractions.  Methods: The indirect enzyme-linked immunosorbent assay (ELISA) was chosen to evaluate the efficacy of heterologous anticrotalic serum produced by Instituto Butantan (Brazil) in recognizing the twenty-two Crotalus durissus collilineatus venoms and the pool of them. Moreover, the venom...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Snake venoms; Crotalus durissus collilineatus; Antivenomic; Crotalic antivenom; Individual variation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100601

Ballast water: a review of the impact on the world public health
Provedor de dados: 80
Autores: Takahashi,CK; Lourenço,NGGS; Lopes,TF; Rall,VLM; Lopes,CAM.
Since the nineteenth century ships have been using ballast water (BW) for safety, stability, propulsion and maneuverability, as well as to redress loss of fuel weight and water consumption, and to maintain structural stress at acceptable levels. Ballast water has been spreading many non-native species around the globe, but little is known about the extent and potential significance of ship-mediated transfer of microorganisms. The global movements of ballast water by ships create a long-distance dispersal mechanism for human pathogens that may be important in the worldwide distribution of microorganisms, as well as for the epidemiology of waterborne diseases. Only a few studies have been carried out on this subject, most of them involving ballast water...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Public health; Ballast water; Vibrio cholerae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300002

Cytotoxic effect of ICD-85 (venom-derived peptides) on MDA-MB-231 cell line
Provedor de dados: 80
Autores: Zare Mirakabadi,A; Mahdavi,S; Koohi,MK; Taghavian,M.
Since 1987, when chemopreventive testing programs began, more than 1,000 agents and agent combinations have been selected and evaluated in preclinical studies of chemopreventive activity against various types of cancers. In the present study we aimed to provide quantitative and qualitative characterization of biological and pharmacological activities of ICD-85 on MDA-MB-231 cell line (a highly invasive breast cancer cell line) in order to gain a better understanding of the cytotoxic and apoptotic effects of this compound. For this study, the MDA-MB-231 cell line was used and the effect of ICD-85 was assayed by measuring the activity of the cytosolic enzyme lactate dehydrogenase (LDH), released into the culture medium after membrane damage. Morphological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venom peptides; MDA-MB-231; Anticancer; Necrosis; Cytotoxic.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400005

Purification and n-terminal sequencing of two presynaptic neurotoxic PLA2, neuwieditoxin-I and neuwieditoxin-II, from Bothrops neuwiedi pauloensis (jararaca pintada) venom
Provedor de dados: 80
Autores: Borja-Oliveira,C. R.; Kassab,B. H.; Soares,A. M.; Toyama,M. H.; Giglio,J. R.; Marangoni,S.; Re,L.; Rodrigues-Simioni,L.
Two presynaptic phospholipases A2 (PLA2), neuwieditoxin-I (NeuTX-I) and neuwieditoxin-II (NeuTX-II), were isolated from the venom of Bothrops neuwiedi pauloensis (BNP). The venom was fractionated using molecular exclusion HPLC (Protein-Pak 300SW column), followed by reverse phase HPLC (µBondapak C18 column). Tricine-SDS-PAGE in the presence or absence of dithiothreitol showed that NeuTX-I and NeuTX-II had a molecular mass of approximately 14 kDa and 28kDa, respectively. At 10µg/ml, both toxins produced complete neuromuscular blockade in indirectly stimulated chick biventer cervicis isolated preparation without inhibiting the response to acetylcholine, but NeuTX-II reduced the response to KCl by 67.0±8.0% (n=3; p&lt;0.05). NeuTX-I and NeuTX-II are probably...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chick biventer cervicis; Loose patch clamp; Nerve-muscle preparation; Neuromuscular junction; Neurotoxicity; PLA2 neurotoxin; Presynaptic action; Bothrops neuwiedi pauloensis; Neuwieditoxin-I; Neuwieditoxin-II.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100008

Comparison of humoral immune response, neutralization capacity of anticrotalic serum in young ovines, clinical and weight evaluation between animals inoculated with Crotalus durissus terrificus venom, natural or cobalt-60-irradiated
Provedor de dados: 80
Autores: Ferreira Junior,R. S.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300017

Cardiac evaluation after experimental intoxication by Amorimia rigida (Malpighiaceae) extracts in rabbits
Provedor de dados: 80
Autores: Borboleta,LR; Silva,EF; Oliveira,MS; Michel,AFRM; Ferraz,VP; Leite,RC; Melo,MM.
Clinical and laboratory changes were evaluated in rabbits after intoxication by Amorimia rigida, a plant that causes sudden death. Nine New Zealand male rabbits, averaging 3.54 kg, were categorized into three groups (n = 3) and received, for eight consecutive days, the equivalent of 30 g/kg dry matter of A. rigida water-soluble (SG) and water-insoluble (IG) extracts via nasoesophageal route. The control group received water. There were no alterations in creatine kinase enzyme (CK), CK myocardial fraction (CKMB) or troponine I (cTnI). None of the animals had clinical or electrocardiographic (conventional and Holter) alterations. There were progressive decreases in the left ventricular ejection fraction and systolic fractional shortening. Doppler...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxic plant; Amorimia rigida; Cardiac muscle profile; Electrocardiography; Echocardiography; Rabbit.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300007

Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp.
Provedor de dados: 80
Autores: Marconi,C.; Cunha,M. L. R. S.; Araújo Jr,J. P.; Rugolo,L. M. S. S.
Coagulase-negative staphylococci (CNS), components of the normal flora of neonates, have emerged as important opportunistic pathogens of nosocomial infections that occur in neonatal intensive care units. Some authors have reported the ability of some CNS strains, particularly Staphylococcus epidermidis, to produce a toxin similar to S. aureus delta toxin. This toxin is an exoprotein that has a detergent action on the membranes of various cell types resulting in rapid cell lysis. The objectives of the present study were to standardize the Polymerase Chain Reaction (PCR) technique for the detection of the gene responsible for the production of delta toxin (hld gene) in staphylococcal species isolated from catheters and blood cultures obtained from neonates,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Delta toxin; PCR; Staphylococcus; Coagulase-negative staphylococci.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200004

Use of Cavia porcellus (guinea pigs) as an experimental model for Leishmania (Viannia) braziliensis
Provedor de dados: 80
Autores: Paula,C. D. R.; Friedman,H.; Sampaio,R. N. R.
Laboratory animals are fundamental to study immunological aspects and the efficacy of new drugs to treat leishmaniasis. However, we do not have practical and good animal models to study leishmaniasis caused by Leishmania (Viannia) braziliensis - L(V)b. In this study, thirty-two experimental animals (Cavia porcellus) were injected in the hind foot with 3x10(5) promastigote forms of L(V)b. The animals were followed for eight weeks. None of the experimental animals developed lesions or presented the parasite in any of the tests performed (histopathological exam, smears, culture, inoculation in hamsters, and polymerase chain reaction).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental leishmaniasis; Cavia; Animal model; American leishmaniasis; Pathogenesis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400017

DNA identification of Leishmania (Viannia) braziliensis in human saliva from a patient with American cutaneous leishmaniasis
Provedor de dados: 80
Autores: Corvalan,FH; Sampaio,RNR; Brustoloni,YM; Andreotti,R; Lima Júnior,MSC.
This study aims to report the amplification of the DNA of Leishmania (V.) braziliensis, using polymerase chain reaction, obtained from the saliva of a patient with American cutaneous leishmaniasis who did not present any lesion in the oral mucosa. Amplification produced fragments of 103 bp, an estimated size employing Leishmania (V.) braziliensis primers (b1 e b2). The present results revealed, for the first time, that the in vitro amplification of Leishmania DNA using samples from the salivary fluid of a patient with American cutaneous leishmaniasis is possible. However, more studies are required with a larger number of participants to evaluate the usefulness of saliva as a non-invasive sample for PCR. The development of such non-invasive technique is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cutaneous leishmaniasis saliva; Leishmania polymerase chain reaction non-invasive diagnosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100013

Pp65 antigenemia for cytomegalovirus infection diagnosis in AIDS patients
Provedor de dados: 80
Autores: Capela,R. C.
Tipo: Info:eu-repo/semantics/article
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400022

Dangerous scorpion fauna of Mali
Provedor de dados: 80
Autores: Goyffon,M; Dabo,A; Coulibaly,SK; Togo,G; Chippaux,JP.
Although the main Malian scorpion species of medical interest, Androctonus amoreuxi, is responsible for severe envenomings and perhaps some deaths, it has hitherto been considered not dangerous for humans. This population is located in the Saharian North-Eastern regions of Mali where it is accompanied by Leiurus quinquestriatus, a well known dangerous species of the Sahara. In the Gao district, divided by the Niger River, less desolate than the Tessalit and Kidal regions, one specimen of the dangerous species Androctonus australis was found. To summarize, Mali harbors at least three dangerous scorpion species: Leiurus quinquestriatus, Androctonus amoreuxi and A. australis, the latter recently having been identified in Mali for the first time. The absence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpions; Mali; Dangerous species; Androctonus sp; Antivenom.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400003

Antibacterial activity of venom from funnel web spider Agelena labyrinthica (Araneae: Agelenidae)
Provedor de dados: 80
Autores: Benli,M; Yigit,N.
Since the number of microorganisms that are resistant to antibiotics has been increasing steadily, the need for combating these pathogens requires new pharmaceutical agents. To produce these substances, new models have been developed in recent decades. In our study, the venom of Agelena labyrinthica (Clerck, 1757) (Araneae: Agelenidae) was tested against ten bacterial strains, specifically, testing 1/100, 1/10 and 1/1 fractions of diluted venom against these bacteria. While the 1/100 dilution was successful in only one of ten bacterial strains, the 1/10 and the 1/1 were effective on six of ten bacterial strains. The most effective results, among these three different concentrations, were observed on Bacillus subtilis. The other five strains that were also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agelena labyrinthica; Venom; Antimicrobial activity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400007

Heparin and commercial bothropic antivenom against the paralyzing effect of Bothrops jararacussu snake venom
Provedor de dados: 80
Autores: Rostelato-Ferreira,S; Rodrigues-Simioni,L; Oshima-Franco,Y.
The crude venom of Bothrops jararacussu (Bjssu) is known to induce muscular paralysis in vitro. Many studies have shown that various substances, including heparin, neutralize the damage caused by snake venom. In the present study, the ability of heparin (Hep) and commercial bothropic antivenom (CBA) to neutralize neuromuscular effects of Bjssu venom, at different time-points, was analyzed. Mouse phrenic nerve-diaphragm preparation was used through a conventional myographic technique, following five different protocols: Group 1 was incubated with Bjssu (40 µg/mL) without any other treatment; Groups 2 and 3 were pretreated with heparin (1 µL/mL) and CBA (120 µL/mL), respectively, for 15 minutes before venom addition; Group 4 after 50% neuromuscular blockade...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararacussu; Heparin; Neutralization; Nerve-muscle preparation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100005

Correlation between serum levels of interleukins 10 and 12 and thrombocytopenia in hepatitis C cirrhotic (class A) patients
Provedor de dados: 80
Autores: Abdallah,N; Abdel Aziz,HK; Hamed,NA; Gamal,M.
Hepatitis C virus (HCV) patients commonly have low platelet counts; however, the exact role of HCV infection in thrombocytopenia is unknown. This work aimed to study the serum levels of interleukins (IL) 10 and 12 in patients with mild and moderate thrombocytopenia associated with chronic hepatitis C infection. Our study included 15 patients with chronic HCV infection and newly diagnosed isolated autoimmune thrombocytopenia (Group I) and 15 patients with chronic HCV infection and normal platelet count as controls (Group II). All patients were examined for personal history and clinical aspects, complete blood count, bone marrow aspiration, liver function tests, HCV antibody assay by ELISA and polymerase chain reaction (PCR), abdominal ultrasound,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interleukin-10; Interleukin-12; Thrombocytopenia; Hepatitis C activity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300012

Use of repellents for honeybees (Apis mellifera L.) in vitro in the yellow passion-fruit (Passiflora edulis Deg) crop and in confined beef cattle feeders
Provedor de dados: 80
Autores: Nicodemo,D.; Nogueira Couto,R. H.
The presence of Apis mellifera in places such as candy and soft drink factories, restaurants, and ice-cream shops has been a concern to many people. In the yellow passion-fruit crop, Apis mellifera is able to collect all anther pollen but has no active role in pollination. Honeybees also visit animal feeders with chopped sugar cane, preventing the cattle from eating. This work studied the effect of natural and synthetic substances as Apis mellifera repellents in vitro in the yellow passion-fruit crop and in confined beef cattle feeders. There was a repellent effect in vitro with the following substances in decreasing order: tobacco, rue, garlic, parsley, and century plant extracts; average effect was twenty-five minutes. For the yellow passion-fruit,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Honeybees; Repellents; Yellow passion fruit crop; Beef cattle feeder; Pheromones.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000100006

Leptospira spp. antibodies in captive coatis (Nasua nasua Storr, 1780) (Carnivora: Procyonidae)
Provedor de dados: 80
Autores: Langoni,H; Kawaguchi,MF; Oshika,JC; Da Silva,RC; Teixeira,CR.
Leptospirosis is a worldwide infection that affects many species, including wild animals. The present study aimed to detect Leptospira spp. antibodies in 17 captive coatis (Nasua nasua) by the microscopic agglutination test (MAT). Nine (52.94%) animals tested positive and the following serovars were identified: Copenhageni (22.22%), Shermani (22.22%), Andamana (11.11%), Wolfii (11.11%) and Pyrogenes (11.11%). Two samples presented coagglutination, one (11.11%) for Hebdomadis and Wolfii, and another (11.11%) for Hebdomadis, Hardjo and Wolfii. The current study revealed the presence of the infection even in animals without any clinical signs, reinforcing the possibility that wild animals in captivity may be infected by leptospire serovars, thus enabling...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nasua nasua; Leptospira spp.; Captive coatis; Antibodies; Microscopic agglutination test.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400013

Cytokine profile and clinical metabolic alterations in HIV-1 infected individuals with and without lipodistrophy
Provedor de dados: 80
Autores: Pontes-Cardoso,L. C. R.; Souza,L. R.; Peraçoli,M. T. S.; Pereira,P. C. M.
The extensive use of Highly Active Antiretroviral Therapy (HAART) has transformed HIV infection into a chronic condition. Thus, metabolic alterations including lipodystrophy and dyslipidemia have been associated with the use of such medications. The objective of the present study was to analyze clinical metabolic alterations and the profile of TNF-alpha, IFN-Y, IL-2, IL-10, and TNF-alpha type II soluble receptor in serum of HIV-1 individuals with and without lipodystrophy. Eighty-four adults were evaluated, 42 males and 42 females, mean age 37 years, and HAART time of at least 15 months. Two groups were formed, G1: 42 individuals with lipodystrophy, and G2: 42 without lipodistropy. From the HAART used, stavudine was more associated with the lipodystrophy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipodystrophy; HIV-1; Hypertriglyceridemia; Cytokines.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200009

Hemolytic, anticancer and antigiardial activity of Palythoa caribaeorum venom
Provedor de dados: 80
Autores: Lazcano-Pérez,Fernando; Zavala-Moreno,Ariana; Rufino-González,Yadira; Ponce-Macotela,Martha; García-Arredondo,Alejandro; Cuevas-Cruz,Miguel; Gómez-Manzo,Saúl; Marcial-Quino,Jaime; Arreguín-Lozano,Barbarín; Arreguín-Espinosa,Roberto.
Abstract  Background Cnidarian venoms and extracts have shown a broad variety of biological activities including cytotoxic, antibacterial and antitumoral effects. Most of these studied extracts were obtained from sea anemones or jellyfish. The present study aimed to determine the toxic activity and assess the antitumor and antiparasitic potential of Palythoa caribaeorum venom by evaluating its in vitro toxicity on several models including human tumor cell lines and against the parasite Giardia intestinalis.  Methods The presence of cytolysins and vasoconstrictor activity of P. caribaeorum venom were determined by hemolysis, PLA2 and isolated rat aortic ring assays, respectively. The cytotoxic effect was tested on HCT-15 (human colorectal adenocarcinoma),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cnidarian; Palythoa caribaeorum; Cytotoxin; Antitumoral effect; Giardiasis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100306

Septic arthritis: immunopathogenesis, experimental models and therapy
Provedor de dados: 80
Autores: Colavite,Priscila Maria; Sartori,Alexandrina.
Septic arthritis is an inflammatory disease of the joints that is started by an infection whose most common agent is Staphylococcus aureus. In this review we discuss some of the most arthritogenic bacterial factors and the contribution of innate and specific immune mechanisms to joint destruction. Special emphasis is given to the induction of experimental arthritis by S. aureus in mice. The improvement of therapy by association of antibiotics with down-modulation of immunity is also included.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Septic arthritis; Staphylococcus aureus; Mice.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200205

Yellow fever in Africa and the Americas: a historical and epidemiological perspective
Provedor de dados: 80
Autores: Chippaux,Jean-Philippe; Chippaux,Alain.
Abstract Yellow fever was transported during the slave trade in the 15th and 16th centuries from Africa to the Americas where the virus encountered favorable ecological conditions that allowed creation of a sustainable sylvatic cycle. Despite effective vector control and immunization programs for nearly a century, yellow fever epidemics reemerged in many Latin American countries, particularly Brazil. The emergence or reemergence of vector-borne diseases encompasses many intricate factors. Yellow fever outbreaks occur if at least three conditions are fulfilled: the introduction of the virus into a non-immune human community, presence of competent and anthropophilic vectors and insufficiency of prevention and/or adequate management of the growing outbreak....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yellow fever; Aedes aegypti; Haemagogus sp.; Sabethes sp. Vector; Arbovirus; Epidemiology; Brazil; Latin America; Africa.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100207

Characterization of insecticidal peptides from venom Australian funnel-web spiders
Provedor de dados: 80
Autores: Vonorax,E. J.; Tyler,M. I.; Atkinson,R. K.; Howden,M. E. H.
Australian funnel-web spiders are relatively large primitive hunting spiders. Male Atrax robustus spiders have been responsible for a number of human deaths. Venom was collected from the species Hadronyche infensa (Hickman) [female], H. formidabilis [male and female], H. versuta [female], and A. robustus (Cambridge) [male] and was fractionated by high performance liquid chromatography. This resulted in the isolation and purification of a homologous series of 7 insecticidal peptides of relatively low molecular mass (approximately 4kDa). The amino acid sequences of these toxins consisted of 36 or 37 amino acids and were named atracotoxins. For the major bioassay of these toxins, we used the cotton bollworm, Helicoverpa armigera (Hubner), due to the great...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Australian funnel-web spiders; Hadronyche and Atrax species; Venom; Insecticidal toxins; Peptides; Helicoverpa armigera.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200005

Oral Presentations
Provedor de dados: 80
Tipo: Info:eu-repo/semantics/other
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200016

Purification procedure for the isolation of a P-I metalloprotease and an acidic phospholipase A 2 fromBothrops atrox snake venom
Provedor de dados: 80
Autores: Menaldo,Danilo L.; Jacob-Ferreira,Anna L.; Bernardes,Carolina P.; Cintra,Adélia C. O.; Sampaio,Suely V.
Background Snake venoms are complex mixtures of inorganic and organic components, mainly proteins and peptides. Standardization of methods for isolating bioactive molecules from snake venoms is extremely difficult due to the complex and highly variable composition of venoms, which can be influenced by factors such as age and geographic location of the specimen. Therefore, this study aimed to standardize a simple purification methodology for obtaining a P-I class metalloprotease (MP) and an acidic phospholipase A2 (PLA 2 ) from Bothrops atroxvenom, and biochemically characterize these molecules to enable future functional studies.Methods To obtain the toxins of interest, a method has been standardized using consecutive isolation steps. The purity level of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venoms; Bothrops atrox; Toxins; Metalloprotease; Phospholipase A2; Isolation; Characterization; Chromatography.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100337

Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768)
Provedor de dados: 80
Autores: Netto,D. P.; Chiacchio,S. B.; Bicudo,P. L.; Alfieri,A. A.; Balarim,M. R. S.; Nascimento,N.
Natural (NV) and Cobalto60-irradiated (IrV) Crotalus durissus terrificus venom were used to evaluate serum production capacity of sheep and possible hematological and biochemical effects. Freeze-dried venom aliquots were diluted in acidified saline solution (NaCl 150 mM, pH 3.0) and irradiated by a Cobalt 60 source at a dose of 5.54 x 102 Gy/h and a concentration of 2.000 Gy. Twelve sheep were divided into two groups of six animals. One group received irradiated venom (IrV) and the other natural venom (NV). Three antigen doses (venom) were administered at monthly intervals. Blood samples were collected weekly for analysis of serum neutralization potency and capacity, complete blood count (CBC), total plasma protein, fibrinogen, albumin, and globulin. At...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematology; Gamma radiation; Irradiated crotalic venom; Crotalic antiserum; Ovine; Crotalus durissus terrificus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000100004

Comparison of two purified toxic fractions from Mesobuthus eupeus scorpion venom
Provedor de dados: 80
Autores: Shirmardi,SP; Shamsaei,M; Gandomkar,M; Saniei,E; Ghannadi,M; Zare,A.
Iranian scorpions belong mainly to the Buthidae and Scorpionidae families, distributed into 16 genera and 25 species. In Iran, similar to other parts of the world, there are a few known species of scorpions responsible for severe envenoming; amongst which Mesobuthus eupeus is the most common. Its venom contains several toxin fractions that may affect the ion channel. In the present study purification, labeling and biological evaluation of M. eupeus venom are described. For separation, soluble venom was loaded on a chromatography column packed with Sephadex G-50 gel. Subsequently, the fractions were collected according to UV absorption at a wavelength of 280 nm. Toxic fraction (F3) was loaded on an anionic ion exchanger resin and then on a cationic resin....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesobuthus eupeus; Venom; Purification; Chromatography; Radiolabeling; Biodistribution.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400015

Prevalence and risk factors for human toxoplasmosis in a rural community
Provedor de dados: 80
Autores: Marques,JM; da Silva,DV; Correia,NAB; Velásquez,G; da Silva,RC; Langoni,H; da Silva,AV.
Toxoplasma gondii infection may lead to important pathological questions, especially in rural areas, where several sources of infection exist. Therefore, it is important to determine risk factors in order to establish adequate prophylactic measures. The present study aimed to assess the prevalence and risk factors involved in human toxoplasmosis infection in a rural community, in Eldorado, Mato Grosso do Sul State, Brazil. This community was composed of 185 farms - with 671 inhabitants - from which 20 were randomly chosen. In these farms, blood samples were collected from rural workers, who also answered a risk factor questionnaire. Serum samples were analyzed by means of direct agglutination test for the detection of anti-Toxoplasma gondii antibodies....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Prevalence; Risk factors; Rural community.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400010

Correlation between annual activity patterns of venomous snakes and rural people in the Niger Delta, southern Nigeria
Provedor de dados: 80
Autores: Akani,Godfrey C; Ebere,Nwabueze; Franco,Daniel; Eniang,Edem A; Petrozzi,Fabio; Politano,Edoardo; Luiselli,Luca.
Background Venomous snakes are among the most serious health hazards for rural people in tropical regions of the world. Herein we compare the monthly activity patterns of eight venomous snake species (Elapidae and Viperidae) with those of rural people in the Niger Delta area of southern Nigeria, in order to identify the periods of highest potential risk for persons, and the human group actually at greater risk of snakebite. Results We documented that above-ground activity of all venomous snakes peaked in the wet season, and that high snake activity and high human activity were most highly correlated between April and August. In addition, we documented that women and teenagers were at relatively higher risk of encountering a venomous snake than adult males,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monthly activity; Venomous snakes; Rural people; Nigeria.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100301

Comparative in vitro analysis of different antimicrobial agent action/activity against Pseudomonas aeruginosa strains isolated from human infections
Provedor de dados: 80
Autores: Ferrari,N. B. M.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100017

Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis
Provedor de dados: 80
Autores: Camargo,JB; Langoni,H; Troncarelli,MZ; Machado,JG; Lucheis,SB; Padovani,CR.
Canine visceral leishmaniasis (CVL) is endemic in numerous Brazilian regions. The greatest difficulty in controlling the disease is the diagnostic limitation. In the present study, the most common tests employed for visceral leishmaniasis diagnosis were compared: immunofluorescence antibody test (IFAT), immunoenzymatic assay (ELISA), direct parasitological examination and polymerase chain reaction (PCR). Samples of lymph node aspirates and blood were collected from 100 dogs that lived in an endemic area (Bauru city, São Paulo state) and from 100 negative controls from a non-endemic area (Botucatu city, São Paulo state). Specificity of both IFAT and PCR was 100% whereas ELISA was 99%. Sensitivities were 97.77, 93.33 and 91.11% respectively for IFAT, ELISA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Dog; Diagnosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300007

Clinical and cardiovascular alterations produced by scorpionic envenomation in dogs
Provedor de dados: 80
Autores: Cordeiro,F. F.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300014

Interleukin-15: its role in microbial infections
Provedor de dados: 80
Autores: Bannwart,C.F.; Nakaira,E.T.; Sartori,A.; Peraçoli,M.T.S.
Interleukin-15 (IL-15) is a pleiotropic cytokine which regulates the proliferation, survival and the secretory activities of many distinct cell types in the body. This cytokine is produced by macrophages and many other cell types in response to infectious agents; it controls growth and differentiation of T and B lymphocytes, activation of Natural Killer (NK) and phagocytic cells, and contributes to the homeostasis of the immune system. The present review focuses on the biological and modulatory effects of IL-15 in microbial infections and shows that this cytokine may play a role in the host defense against infections by inducing activation of effector cells from both innate and adaptive immune system.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interleukin-15; Microbial infections; Innate immunity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300002

Lepromatous leprosy and perianal tuberculosis: a case report and literature review
Provedor de dados: 80
Autores: Parise-Fortes,Maria Rita; Lastória,Joel Carlos; Marques,Silvio Alencar; Putinatti,Maria Stella Ayres; Stolf,Hamilton Ometto; Marques,Mariângela Ester Alencar; Haddad,Vidal.
Leprosy is a chronic infectious disease caused by Mycobacterium leprae, a microorganism that usually affects skin and nerves. Although it is usually well-controlled by multidrug therapy (MDT), the disease may be aggravated by acute inflammatory reaction episodes that cause permanent tissue damage particularly to peripheral nerves. Tuberculosis is predominantly a disease of the lungs; however, it may spread to other organs and cause an extrapulmonary infection. Both mycobacterial infections are endemic in developing countries including Brazil, and cases of coinfection have been reported in the last decade. Nevertheless, simultaneous occurrence of perianal cutaneous tuberculosis and erythema nodosum leprosum is very rare, even in countries where both...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Mycobacteria; Leprosy; Tuberculosis; Erythema nodosum leprosum.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200506

Postprandial thermogenesis in Bothrops moojeni (Serpentes: Viperidae)
Provedor de dados: 80
Autores: Stuginski,DR; Fernandes,W; Tattersall,GJ; Abe,AS.
Snakes that can ingest prey that are proportionally large have high metabolic rates during digestion. This great increase in metabolic rate (specific dynamic action - SDA) may create a significant augment in the animal's body temperature. The present study investigated postprandial thermogenesis in Bothrops moojeni. Briefly, two groups of snakes were fed meals equivalent to 17 ± 3% and 32 ± 5% of their body weight and were observed for 72 hours, in which thermal images of each snake were taken with an infrared camera in a thermostable environment with a constant air temperature of 30°C. The results showed a significant increase in snake surface temperature, with a thermal peak between 33 and 36 hours after feeding. The meal size had a great impact on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops; Thermogenesis; Feeding behavior; Digestion.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300008

Cytomegalovirus and Toxoplasma gondii seroprevalence in a Brazilian liver transplant waiting
Provedor de dados: 80
Autores: Almeida,R. A. M. B.; Silva,G. F.; Llanos,J. C.; Winckler,C. C.; Gomes,M. R. B.; Biagioni,D. S.; Paula,R. C. C.; Meira,D. A.; Bakonyi,A.
Cytomegalovirus (CMV) disease is a major cause of morbidity and mortality in solid organ transplantation. Disseminated toxoplasmosis after liver transplantation is a rare but fatal event. Serologic screening of the donor and the recipient is essential to prophylactic management, early diagnosis and therapeutic strategies to minimize the consequences of these infections. The aim of the present study was to determine the seroprevalence of CMV and Toxoplasma gondii (TG) in a Brazilian liver transplant waiting list (LTWL). Serological data were collected from 44 candidates on the LTWL between May 2003 and November 2004. Serological investigation of antibodies IgM and IgG against CMV (anti-CMV) and TG (anti-T. gondii) was performed using fluorometry commercial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytomegalovirus; Liver transplantation; Toxoplasmosis; Toxoplasma gondii; Seroprevalence; Waiting list.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400016

Antibacterial activity of Apis mellifera L. propolis collected in three regions of Kenya
Provedor de dados: 80
Autores: Muli,E. M.; Maingi,J. M.
The present study aimed at investigating the susceptibility of the microorganisms Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis to ethanolic extracts of propolis (EEP) from three regions of Kenya (Taita, Tana and Samburu). Propolis was extracted using four different concentrations of ethanol: pure, 70%, 50%, and 30%. Ethanol (70%) and Streptomycin were used as controls. The agar diffusion method using filter paper disks was employed. Antibacterial activity was determined as an equivalent of the inhibition zones diameters (in millimeters) after incubation at 37°C for 24h. Significant differences in the antibacterial activities of propolis were observed among the three regions, depending on the test...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apis mellifera; Propolis; Antibacterial agents; Disk diffusion antimicrobial tests.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300008

Chemical composition and antibacterial activity of Brazilian propolis essential oil
Provedor de dados: 80
Autores: Oliveira,AP; França,HS; Kuster,RM; Teixeira,LA; Rocha,LM.
The present study aimed at investigating the chemical composition of essential oil extracted from Brazilian propolis and the susceptibility of Staphylococcus aureus, Staphylococcus epidermides, Streptococcus pyogenes and Escherichia coli to this substance. The essential oil was obtained by steam distillation of propolis and examined by gas chromatography/mass spectrometry (GC/MS). In addition, the agar diffusion method using filter paper disks was employed. Antibacterial activity was measured as equivalent diameters of inhibition zones (in millimeters) after incubation at 37º C for 24 hours. From the 26 identified constituents, β-caryophyllene (12.7%), acetophenone (12.3%) and β-farnesene (9.2%) were found to be major components. New components, namely...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apis mellifera; Propolis essential oil; Terpenoids; Antibacterial activity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100012

Molecular cloning of a hyaluronidase fromBothrops pauloensisvenom gland
Provedor de dados: 80
Autores: Castanheira,Letícia Eulalio; Rodrigues,Renata Santos; Boldrini-França,Johara; Fonseca,Fernando PP; Henrique-Silva,Flávio; Homsi-Brandeburgo,Maria I; Rodrigues,Veridiana M.
Background Hyaluronate is one of the major components of extracellular matrix from vertebrates whose breakdown is catalyzed by the enzyme hyaluronidase. These enzymes are widely described in snake venoms, in which they facilitate the spreading of the main toxins in the victim’s body during the envenoming. Snake venoms also present some variants (hyaluronidases-like substances) that are probably originated by alternative splicing, even though their relevance in envenomation is still under investigation. Hyaluronidases-like proteins have not yet been purified from any snake venom, but the cDNA that encodes these toxins was already identified in snake venom glands by transcriptomic analysis. Herein, we report the cloning and in silicoanalysis of the first...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alternative splicing; Hyaluronidase-like; Snake venom.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200330

Evaluation of the placental transmission of genotypically distinct Toxoplasma gondii strains in Balb/c mice
Provedor de dados: 80
Autores: Pezerico,S. B.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200012

Experimental infection with Leishmania chagasi in immunosuppressed Balb/c mice: cytokines and parasite burdens
Provedor de dados: 80
Autores: Hoffmann,JL; Machado,JG; Gaio,FC; Dias-Melicio,LA; Langoni,H.
The immune response in leishmaniasis may result in a polarization of the T lymphocyte subpopulation, altering cell phenotype and resulting in immune protection or disease exacerbation. Leishmania may persist in the body either during asymptomatic infections or after treatment, which represents high risk under immunosuppression. The objective of this study was to evaluate the effect of infection with immunosuppression by dexamethasone associated with pentoxifylline on animal weight, spleen weight, spleen and hepatic parasitic load and immunopathology, as well as the IFN-γ and IL-10 production in spleen cell culture of Balb/c mice infected with Leishmania chagasi. The infection did not cause body weight gain in animals, but both the weight and size of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania chagasI; Immunosuppression; Cell-mediated response; Parasite burden; Experimental infection.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300004

Antibacterial potential of a basic phospholipase A2(VRV-PL-VIIIa) from Daboia russelii pulchella (Russell's viper) venom
Provedor de dados: 80
Autores: Sudharshan,Shivalingaiah; Dhananjaya,Bhadrapura Lakkappa.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bactericidal; Antibiotics; Drug; Human pathogenic bacteria.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100322

Neolignans isolated from twigs of Nectandra leucantha Ness &amp;amp; Mart (Lauraceae) displayed in vitro antileishmanial activity
Provedor de dados: 80
Autores: Grecco,Simone S; Costa-Silva,Thais A; Sousa,Fernanda S; Cargnelutti,Stefano B; Umehara,Eric; Mendonça,Poliana S; Tempone,Andre G; Lago,Joao Henrique G.
Abstract  Background: The therapeutic arsenal for the treatment of Leishmaniasis is limited and includes toxic compounds (antimonials, amphotericin B, pentamidine and miltefosine). Given these aspects, the search for new compounds based on floristic biodiversity is crucial. In the present work, we report the isolation, characterization and antileishmanial activity of six related neolignans (1–6) of bioactive extract from Nectandra leucantha (Lauraceae) twigs.  Methods: Dried and powdered twigs of N. leucantha were exhaustively extracted using n-hexane. The crude extract was dereplicated by HPLC/HRESIMS and subjected to column chromatography to yield pure compounds 1–6. Their chemical structures were identified via NMR and comparison of obtained data with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nectandra leucantha; Leishmania (L.)infantum; Antileishmanial activity; Neolignans.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100318

Study of the relationship between Androctonus crassicauda (Oliver, 1807; scorpiones, buthidae) venom toxicity and telson size, weight and storing condition
Provedor de dados: 80
Autores: Ozkan,O.; Adiguzel,S.; Yakistiran,S.; Filazi,A.
Scorpions are included in the order Scorpiones; class Arachnida. Lethal scorpions are mostly of the Buthidae family. Among these, species belonging to Androctonus, Leiurus and Mesobuthus genera cause most scorpion envenomations in Turkey. This study was performed aiming the production of antivenom by using Androctonus crassicauda telsons. Venom toxicity is related to telson weight, size, and storing condition (open or closed). Telsons of A. crassicauda were collected in Southeastern Anatolia (especially in Harran town, Sanliurfa), Turkey. They were separated according to weight, size, and storing condition - open (a) and closed (b). Venom solution was prepared by maceration of telsons. Swiss albino mice were used to determine the lethal dose 50% (LD50),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion Androctonus crassicauda (Oliver; 1807) telson size and weight LD50; Acute toxicity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200011

Effects of Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) venom on rat metabolism
Provedor de dados: 80
Autores: Ozkan,O.; Bakir,F.; Adiguzel,S.
Scorpions are venomous arthropods of the Arachnida class and are considered relatives of spiders, ticks and mites. There is not any study about the biochemical effects of Androctonus crassicauda (Olivier, 1807) venom. Therefore, in the present study, we aimed at evaluating the toxicity of the venom from A. crassicauda, which is responsible for a number of deaths of infants, children and adults in tropical and subtropical countries. For this purpose, rats (n=35) were divided into seven groups of five animals each; venom solutions (250µg/kg) were subcutaneously injected into rats; blood samples were taken from each animal at various times; and serum biochemical parameters were measured (levels of total proteins, total bilirubin, albumin, globulin, urea,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpions; Androctonus crassicauda; Venom; Rats; Biochemical parameters.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100004

Insecticidal activity of venomous saliva from Rhynocoris fuscipes (Reduviidae) against Spodoptera litura and Helicoverpa armigera by microinjection and oral administration
Provedor de dados: 80
Autores: Sahayaraj,K; Vinothkanna,A.
Rhynocoris fuscipes is a potential predator of many economically important pests in India. In the present study, its venomous saliva (VS) was collected by milking and diluted with HPLC grade water to different concentrations (200, 400, 600, 800 and 1000 ppm). Microinjection of Rhynocoris fuscipes VS was more toxic than its oral administration in Helicoverpa armigera (cotton bollworm) and Spodoptera litura (tobacco cutworm). Thus, R. fuscipes VS was found to be toxic to third instar S. litura and H. armigera with respective LD50s of 846.35 and 861.60 ppm/larva at 96 hours after microinjection. The current results showed that VS of Rhynocoris fuscipes caused mortality of H. armigera and S. litura. Active peptides from VS may be isolated, identified and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salivary venom; Microinjection; Oral administration; Mortality; Rhynocoris fuscipes; Biological control.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400016

Rabies in the big fruit-esting bat Artibeus lituratus from Botucatu, Southeastern Brazil
Provedor de dados: 80
Autores: Langoni,H.; Lima,K.; Menozzi,B. D.; Silva,R. C.
Rabies is a viral disease of mammals transmitted through the bite of a rabid animal. A frugivorous adult male bat, Artibeus lituratus, family Phyllostomidae, was diagnosed as positive to rabies by direct immunofluorescence (DIF) and mouse inoculation test (MIT) of the bat's brain, both performed at the School of Veterinary Medicine and Animal Husbandry - FMVZ, UNESP, Botucatu, São Paulo State, Brazil. The animal collided with the window of a commercial establishment in the urban area during the day. With regard to DIF, a high amount of Negri bodies of several sizes was observed in the brain. The spleen and right kidney presented some Negri bodies too. In relation to MIT, the mice presented paralysis in the 7th day, and died in the day after with several...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Southeastern Brazil; Rabies; Phyllostomidae; Frugivorous bat.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100010

Antivenom therapy: efficacy of premedication for the prevention of adverse reactions
Provedor de dados: 80
Autores: Morais,Victor.
Abstract: Antivenoms or antitoxins have been effectively used for more than a century. During this time, these products have always proven to be highly effective in the treatment of infections and envenomations. However, antivenoms did not exhibit good safety results in their initial applications. After many improvements, antivenoms have substantially better safety profiles but still have some side effects. Due to the occurrence of adverse reactions, the practice of using premedication with the intent to decrease side effects has become accepted or mandatory in many countries. The drugs used for premedication belong to the histamine H1 antagonist, glucocorticoid and catecholamine groups. Currently, this practice is being questioned due to low or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Premedication; Snakebite accident; Adverse reactions; Hydrocortisone; Antihistaminic; Adrenaline.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100203

Oviposition sequence and offspring of mated and virgin females of Cotesia flavipes (Hymenoptera: Braconidae) parasitizing Diatraea saccharalis larvae (Lepidoptera: Crambidae)
Provedor de dados: 80
Autores: Scaglia,M.; Chaud-Netto,J.; Brochetto-Braga,M. R.; Ceregato,S. A.; Gobbi,N.; Rodrigues,A.
Large scale mass rearing of natural enemies has been a mean of improving biological control in the sugarcane intensive agriculture. Among them, Cotesia flavipes, a gregarious koinobiont endoparasitoid, was imported by Brasil to control caterpillars of the sugarcane borer Diatraea saccharalis. The C. flavipes larval development depends on its association with polydnavirus, which blocks the host defense reaction. To verify if the oviposition sequence (1st, 2nd or 3rd) and the female condition (mated or virgin) interfere in the number of C. flavipes descendents, 4th instar caterpillars of D. saccharalis were parasitized. Analysis of the data showed that: a) there is an inverse correlation between the parasitism efficiency and the host reaction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cotesia flavipes; Diatraea saccharalis; Oviposition sequence; Encapsulation; Chrysalises; Parasitism.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300007

Consequences of Mesobuthus tamulus gangeticus (Pocock, 1900) envenomation in albino mice
Provedor de dados: 80
Autores: Chaubey,MK.
The present study aimed to investigate the effects of Mesobuthus tamulus gangeticus Pocock (Buthidae) venom on albino mice (NIH strain). Whole venom was obtained by electrical stimulation and its toxicity was determined in albino mice by subcutaneous envenomation. The venom LD50 was 2.5 mg kg-1 of mouse body weight. Toxic effects on different biochemical and enzymatic parameters in blood serum and other tissues of albino mice were determined after experimental envenomation with sublethal doses of M. tamulus gangeticus venom. Increased levels of glucose, uric acid and cholesterol, as well as decreased serum total proteins, were observed at 2 and 4 hours after the envenomation. In the liver and muscles, glycogen content dropped after venom injection....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesobuthus tamulus gangeticus; Scorpion venom; Hyperglycemia; Serum enzymes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300011

Jorge Lobo' s immunopathology: cell composition of the inflammatory infiltrate and cytokine quantification in mononuclear cell supernatant and serum
Provedor de dados: 80
Autores: Vilani-Moreno,F. R.
Tipo: Info:eu-repo/semantics/other
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300013

The History of Venomous Spider Identification, Venom Extraction Methods and Antivenom Production: a Long Journey at the Butantan Institute, São Paulo, Brazil
Provedor de dados: 80
Autores: Lucas,Sylvia M.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ctenus; Lycosa; Phoneutria; Latrodectus; Loxosceles.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100206

Cerebral edema associated to scorpion sting: a two-case sting report
Provedor de dados: 80
Autores: Romero,N. O.; Hernández,T. J. M.
Scorpionism is a public health problem in some places in Mexico. The clinical symptoms of envenomation by scorpion sting are by sympathetic and parasympathetic stimulation, developing systemic and local symptoms. The Central Nervous System (CNS) is one of the organs that are affected. In some cases, cerebral edema develops. In this report we present two pediatric cases with the association of envenomation by scorpion sting and cerebral edema. The first case developed severe cerebral edema, which progressed to a fatal outcome; and the other case developed mild cerebral edema with a satisfactory evolution. The pathophysiology of this complication is not well known and probably is the consequence of hypoxia, secondary to respiratory failure, laryngospasm and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebral edema; Scorpion sting.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400015

Pharmacological characterization of rat paw edema induced by Cerastes gasperettii (cerastes) venom
Provedor de dados: 80
Autores: Al-Asmari,A. K.; Abdo,N. M.
Inflammatory response induced by the venom of the Arabian sand viper Cerastes gasperettii was studied by measuring rat hind-paw edema. Cerastes gasperettii venom (CgV, 3.75-240 µg/paw), heated for 30s at 97°C, caused a marked dose and time-dependent edema in rat paw. Response was maximal 2h after venom administration and ceased within 24h. Heated CgV was routinely used in our experiments at the dose of 120 µg/paw. Among all the drugs and antivenoms tested, cyproheptadine and 5-nitroindazole were the most effective in inhibiting edema formation. Aprotinin, mepyramine, dexamethasone, diclofenac, dipyridamole, Nomega-nitro-L-arginine, quinacrine, and nordihydroguaiaretic acid showed statistically (p&lt;0.001) significant inhibitory effect, but with variations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammatory mediators; Cerastes gasperettii venom; Edema; Antagonist; Antivenom.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300005

In the picture: disulfide-poor conopeptides, a class of pharmacologically interesting compounds
Provedor de dados: 80
Autores: Lebbe,Eline K. M.; Tytgat,Jan.
Abstract During evolution, nature has embraced different strategies for species to survive. One strategy, applied by predators as diverse as snakes, scorpions, sea anemones and cone snails, is using venom to immobilize or kill a prey. This venom offers a unique and extensive source of chemical diversity as it is driven by the evolutionary pressure to improve prey capture and/or to protect their species. Cone snail venom is an example of the remarkable diversity in pharmacologically active small peptides that venoms can consist of. These venom peptides, called conopeptides, are classified into two main groups based on the number of cysteine residues, namely disulfide-rich and disulfide-poor conopeptides. Since disulfide-poor conotoxins are minor components...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cone snail; Conopressin; Contryphan; Conantokin; Contulakin; Conorfamid; Conophan; Conomap; Conomarphin; Conolysin; ConoGAY; ConoCAP; Cono-NPY.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100203

Immune response to infection by Leptospira interrogans serovar icterohaemorrhagiae in genetically selected mice
Provedor de dados: 80
Autores: Marinho,M.
Tipo: Info:eu-repo/semantics/other
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300010

Tuberculin skin testing in HIV-infected patients in Campo Grande, Mato Grosso do Sul State, Brazil
Provedor de dados: 80
Autores: De Oliveira,SMVL; Paniago,AMM; Bonecini-Almeida,MG; Bertoni,N; Stabile,AC; Da Cunha,RV; De Oliveira,OA; Druzian,AF; Negri,AC; Andreotti,R.
A cross-sectional study on HIV/AIDS was carried out in 108 outpatients from the university hospital of the Federal University of Mato Grosso do Sul, Campo Grande, Brazil, from July to December 2008, to investigate latent tuberculosis infection using the tuberculin skin test (TST). The prevalence of positive results was 13.9%. The CD4+ T cell count (p = 0.091) and the diagnosis time (p = 0.010) were statistically significant when compared with TST positivity. In the cohort of HIV/AIDS patients who had latent tuberculosis infection, the median diagnosis time was eight years. Undetectable viral load presented significant association (p = 0.046) with tuberculosis infection. The fact that numerous individuals with HIV/AIDS infection presented a negative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculin skin test; Acquired immunodeficiency syndrome; CD4/CD8 cell count.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200010

Activity of the antiarrhythmic drug amiodarone against Leishmania (L.) infantum: an in vitro and in vivo approach
Provedor de dados: 80
Autores: Pinto,Erika G.; Tempone,Andre G.
Abstract  Background: Considering the high toxicity and limited therapies available for treating visceral leishmaniasis (VL), the drug repositioning approach represents a faster way to deliver new therapies to the market.  Methods: In this study, we described for the first time the activity of a potent antiarrhythmic, amiodarone (AMD), against L. (L.)infantum and its in vitro and in vivo activity.  Results: The evaluation against promastigotes has shown that amiodarone presents leishmanicidal effect against the extracellular form, with an IC50 value of 10 μM. The activity was even greater against amastigotes in comparison with promastigotes with an IC50 value of 0.5 μM. The selectivity index in relation to the intracellular form demonstrated that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Visceral leishmaniasis; Amiodarone; In vitro; In vivo.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100320

Jorge Lobo’s disease immunopathology: cellular composition of the inflammatory infiltrate and cytokine quantification in the supernatant of mononucleated cell cultures and in blood serum
Provedor de dados: 80
Autores: Vilani-Moreno,F. R.
Tipo: Info:eu-repo/semantics/article
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400020

Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature
Provedor de dados: 80
Autores: Tasima,Lídia J.; Serino-Silva,Caroline; Hatakeyama,Daniela M.; Nishiduka,Erika S.; Tashima,Alexandre K.; Sant’Anna,Sávio S.; Grego,Kathleen F.; Morais-Zani,Karen de; Tanaka-Azevedo,Anita M.
Abstract  Background: Crotalus durissus is considered one of the most important species of venomous snakes in Brazil, due to the high mortality of its snakebites. The venom of Crotalus durissus contains four main toxins: crotoxin, convulxin, gyroxin and crotamine. Venoms can vary in their crotamine content, being crotamine-negative or -positive. This heterogeneity is of great importance for producing antivenom, due to their different mechanisms of action. The possibility that antivenom produced by Butantan Institute might have a different immunorecognition capacity between crotamine-negative and crotamine-positive C. durissus venoms instigated us to investigate the differences between these two venom groups.  Methods: The presence of crotamine was analyzed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rattlesnake; Snake venom; Toxins; Venom variation; Antivenom.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100307

Envenomation by the Malagasy colubrid snake Langaha madagascariensis
Provedor de dados: 80
Autores: D'Cruze,NC.
This report documents a case of envenomation by a Malagasy opisthoglyphous snake, Langaha madagascariensis. The snake bit the finger of a healthy adult man who was attempting to record its morphological measurements as part of a biodiversity survey which was being conducted in the extreme north of Madagascar. Symptoms of this case included severe local pain accompanied by observable swelling which lasted for several days. The present observations indicate that bite by L. madagascariensis potentially causes notable physiological effects in humans although the snake is generally calm and reluctant to bite unless provoked.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Madagascar; Colubrid; Langaha madagascariensis; Envenomation; Opisthoglyph; Duvernoy's glands.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300014

Clinical study of natural infection by Trypanosoma cruzi in dogs residing in a rural area in Mato Grosso do Sul State, Brazil
Provedor de dados: 80
Autores: Souza,A. I.
Tipo: Info:eu-repo/semantics/other
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400016

Thyroid evaluation in HIV-1-infected patients
Provedor de dados: 80
Autores: Sgarbi,L. P. S.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100020

Neutralization of pharmacological and toxic activities of Bothrops jararacussu snake venom and isolated myotoxins by Serjania erecta methanolic extract and its fractions
Provedor de dados: 80
Autores: Fernandes,RS; Costa,TR; Marcussi,S; Bernardes,CP; Menaldo,DL; Rodriguéz Gonzaléz,II; Pereira,PS; Soares,AM.
Most of the snakebites recorded in Brazil are caused by the Bothrops genus. Given that the local tissue damage caused by this genus cannot be treated by antivenom therapy, numerous studies are focusing on supplementary alternatives, such as the use of medicinal plants. Serjania erecta has already demonstrated anti-inflammatory, antiseptic and healing properties. In the current study, the aerial parts of S. erecta were extracted with methanol, then submitted to chromatographic fractionation on a Sephadex LH20 column and eluted with methanol, which resulted in four main fractions. The crude extract and fractions neutralized the toxic activities of Bothrops jararacussu snake venom and isolated myotoxins (BthTX-I and II). Results showed that phospholipase A2,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants; Antiophidian properties; Serjania erecta; Bothrops jararacussu snake venom; Myotoxins.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100011

Prevalence and chemotherapy of babesiosis among Lohi sheep in the Livestock Experiment Station, Qadirabad, Pakistan, and environs
Provedor de dados: 80
Autores: Rashid,A; Khan,JA; Khan,MS; Rasheed,K; Maqbool,A; Iqbal,J.
A total of 310 blood smears were collected from sheep of the Livestock Experiment Station, Qadirabad, Sahiwal district, Pakistan, and surrounding areas. The samples were examined microscopically and 30 (9.67%) were positive for babesiosis. The animals were divided into two groups (A and B) for chemotherapy. Group A sheep were treated with diminazene diaceturate while group B animals received imidocarb dipropionate. Drug efficacy was determined by negative blood smear examination. Diminazene diaceturate effectiveness against babesiosis was 80% while that of imidocarb dipropionate was 100%. Hematological studies revealed a significant decrease in hemoglobin concentrations and hematocrit values for Babesia-positive animals compared to healthy controls.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Babesiosis; Sheep; Hemoglobin; Hematocrit; Diminazene; Imidocarb; Pakistan.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400008

Toad poisoning in three dogs: case reports
Provedor de dados: 80
Autores: Barbosa,CM; Medeiros,MS; Riani Costa,CCM; Camplesi,AC; Sakate,M.
Toad poisoning is frequent in dogs, but has been infrequently addressed in published case reports and review articles. Dogs can be poisoned when they bite a toad or otherwise ingest the venom. The venom effects manifest soon after the accident, since the toxin is rapidly absorbed by the mucous membrane of the digestive system. Hospital records of three dogs, diagnosed with toad poisoning, were retrospectively reviewed from January 2005 to July 2007. Poisoned dogs may present only local irritation or systemic signs in the gastrointestinal, cardiac and neurological systems. All three cases presented herein had clinical signs of gastrointestinal alterations including vomiting, sialorrhea and diarrhea. Two dogs developed abnormal cardiac rhythm and two...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Veterinary emergency; Poisoning; Toad venom; Bufotoxin; Bufo sp.; Dog.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400016

Canine visceral leishmaniasis in the Northeast Region of Brazil
Provedor de dados: 80
Autores: Brito,Flávio Gonçalves; Langoni,Helio; Silva,Rodrigo Costa da; Rotondano,Tereza Emmanuelle de Farias; Melo,Marcia Almeida de; Paz,Giselle Souza da.
Abstract  Background  Visceral leishmaniasis (VL) is a zoonosis that affects dogs and other mammals, including humans. Contact with dogs is a major risk factor for humans. This disease is endemic in several regions of Brazil. The aim of this study was to determine the prevalence of Leishmania spp. infection in dogs and to correlate it with possible risk factors.  Methods  Blood samples were collected from 391 dogs of different ages, breeds, and both genders, coming from Campina Grande, Paraíba state, Brazil. An epidemiological questionnaire was employed in order to identify risk factors associated with the disease. Serological tests were performed using indirect immunofluorescence, enzyme-linked immunosorbent assay (ELISA S7®) and polymerase chain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kala-azar; Diagnosis; Dog; Zoonosis; Risk factors.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100311

In vivo and in vitro effects of scorpion venoms in Turkey: a mini-review
Provedor de dados: 80
Autores: Adiguzel,S.
Scorpion stings constitute a common type of accident in Turkey due to the geographic location, climate and socioeconomic structure of this country. Moreover, envenomation cases are considered a public health problem throughout Turkey. Based on data in the literature, the main clinical features of scorpion envenomations are defined as systemic manifestations including sweating, hypertension and vomiting, and among prominent causes of death are cardiorespiratory manifestations, toxic myocarditis and pulmonary edema. The most important health-threatening scorpions found in Turkey are: Androctonus crassicauda, Leiurus quinquestriatus, Mesobuthus gibbosus and Mesobuthus eupeus, all of which belong to the Buthidae family. This study indicates that there is no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpions; Venom; Envenomation; In vivo and in vitro effects; Turkey.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200004

Humoral immune response in dogs and cats vaccinated against rabies in southeastern Brazil
Provedor de dados: 80
Autores: Albas,Avelino; Picolo,Miléia Ricci; Soares,Célio Nereu; Bachega,Hugo Vagner Ulbano; Tarumoto,Mário Hissamitsu.
Background Brazil holds annual nationwide public campaigns to vaccinate dogs and cats against rabies. The presence of rabies antibodies in these animals, which are among the main transmitters of rabies to humans, is a good indicator that they are immunized and protected. Methods In the present study we analyzed 834 serum samples from dogs and cats from the Southeast of Brazil (Presidente Prudente and Dracena cities), 12 months after the 2009 vaccination campaign. We used the technique known as rapid fluorescent focus inhibition test (RFFIT) and considered reactant those sera with values higher 0.5 IU/mL. Results and discussion Reactant sample results in Presidente Prudente were 153 (51.0%) for dogs and 59 (32.6%) for cats, and in Dracena 110 (52.1%) for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Vaccination; Dogs; Cats; Humoral immune response.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100310

Are weight, length and amount of venom related in scorpionfish?
Provedor de dados: 80
Autores: Vieira,RP; Barreiros,JP.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300002

Neglected diseases and radiation
Provedor de dados: 80
Autores: Nascimento,Nanci do.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300001

Loxosceles spider bites in the state of Paraná, Brazil: 1993-2000
Provedor de dados: 80
Autores: Marques-da-Silva,E.; Souza-Santos,R.; Fischer,M. L.; Rubio,G. B. G.
This study presents the epidemiological analysis of 20,699 reported spider bites involving the Loxosceles genus in the state of Paraná, Brazil, from 1993 to 2000. The incidence rate in the period was 290.24/100,000 inhabitants. The results show that 95.04% of the accidents occurred in two Health Regions of the state: greater metropolitan Curitiba (92.9%) and Irati (2.14%). The major incidence was recorded in greater metropolitan Curitiba (559.1/100,000). Loxosceles bites were more frequent in females (61%) than in males. Antivenin therapy was not necessary in 95.4% (19,662) of the cases. From the cases in which information about the time elapsed between the bite and medical treatment was recorded (9,679), 31.8% received treatment from 6 to 48 hours after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Loxosceles; Spiders; Epidemiology; Venomous animals; Brazil.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100009

Identification and characterization of excretory/secretory products of Giardia duodenalis trophozoites
Provedor de dados: 80
Autores: Carvalho,T.
Tipo: Info:eu-repo/semantics/article
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300016

Measurement of IL-10 serum levels in BALB/c mice treated with beta-1,3 polyglucose or sulfadiazine and acutely infected by Toxoplasma gondii
Provedor de dados: 80
Autores: Picka,M. C. M.; Calvi,S. A.; Lima,C. R. G.; Santos,I. A. T.; Marcondes-Machado,J.
Acute infection by Toxoplasma gondii leads to suppression of cell-mediated immunity, facilitating chronic infection. One of the causes of immunosuppression is Interleukin-10 (IL-10) production. Glucan is used to stimulate phagocytosis. Our objective was to study IL-10 induction in male BALB/c mice with acute T. gondii BTU-2 strain infection, glucan immunostimulation, and sulfadiazine treatment. Animals were distributed into 7 groups: G1: infected with T. gondii; G2: infected with T. gondii and treated with sulfadiazine; G3: infected with T. gondii and immunostimulated with glucan; G4: infected with T. gondii, immunostimulated with glucan, and treated with sulfadiazine; G5: imunostimulated with glucan; G6: treated with saline; and G7: treated with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Interleukin-10; Glucan; Immune response; Sulfadiazine.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400012

Experimental Lachesis muta rhombeata envenomation and effects of soursop (Annona muricata) as natural antivenom
Provedor de dados: 80
Autores: Cremonez,Caroline Marroni; Leite,Flávia Pine; Bordon,Karla de Castro Figueiredo; Cerni,Felipe Augusto; Cardoso,lara Aimê; Gregório,Zita Maria de Oliveira; Souza,Rodrigo Cançado Gonçalves de; Souza,Ana Maria de; Arantes,Eliane Candiani.
Abstract  Background  In the Atlantic forest of the North and Northeast regions of Brazil, local population often uses the fruit juice and the aqueous extract of leaves of soursop (Annona muricata L.) to treat Lachesis muta rhombeata envenomation. Envenomation is a relevant health issue in these areas, especially due to its severity and because the production and distribution of antivenom is limited in these regions. The aim of the present study was to evaluate the relevance of the use of soursop leaf extract and its juice against envenomation by Lachesis muta rhombeata.  Methods  We evaluated the biochemical, hematological and hemostatic parameters, the blood pressure, the inflammation process and the lethality induced by Lachesis muta rhombeata snake...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lachesis muta rhombeata; Bushmaster; Natural antivenom; Antiophidic action; Soursop; Annona muricata L.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100310

Renal involvement in visceral leishmaniasis dogs
Provedor de dados: 80
Autores: Soares,M. J. V.; Moraes,J. R. E.; Palmeira Borges,V.; Miyazato,L. G.; Moraes,F. R.
Visceral leishmaniasis (VL) is a zoonosis that affects both animals and man. Dogs are the etiological agent’s main reservoir. The aim of this study was to evaluate the clinical laboratory aspects and renal histopathology of VL dogs. Thirty-four symptomatic (case) and 17 asymptomatic (control) VL seropositive dogs of different breeds, sexes, and ages from Teresina, Piauí State, Brazil, were used. Diagnosis was confirmed by enzyme-linked immunosorbent assay and indirect immunofluorescence test. Clinical and laboratory tests included blood cell count and renal function analysis (urea and creatinine). Animals were subjected to euthanasia and necropsy. Renal fragments were prepared by the usual histological techniques and stained with hematoxylin-eosin and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dogs; Kala-azar; Leishmania; Renal lesion.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400014

Cytokine and antibody production during murine leptospirosis
Provedor de dados: 80
Autores: Marinho,M.; Silva,C.; Lima,V. M. F.; Peiró,J. R.; Perri,S. H. V.
The aim of the present study was to investigate the kinetics of humoral and cellular responses during leptospirosis. We observed that the presence of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) was associated with antibody production and bacterial recovery, and the compromising of both TNF-alpha and IL-6 in the immunopathogenesis of leptospirosis during an experimental infection of BALB/c mice inoculated with Leptospira interrogans serovar Canicola was verified. Results showed higher levels of TNF-alpha and IL-6 in the initial phase of infection, in which the greatest bacterial clearance was observed. However, when the bacterial recovery was compared with the kinetics of the production of antibodies, the results revealed a kinetics...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Cellular immune response; BALB/c mice; Leptospirosis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400006

Lack of association among TNF-α gene expression, -308 polymorphism (G &amp;gt; A) and virulence markers of Helicobacter pylori
Provedor de dados: 80
Autores: Zabaglia,Luanna Munhoz; Ferraz,Mariane Avante; Pereira,Weendelly Nayara; Orcini,Wilson Aparecido; Labio,Roger Willian de; Caleman Neto,Agostinho; Wisnieski,Fernanda; Oliveira,Juliana Garcia de; Smith,Marilia de Arruda Cardoso; Payão,Spencer Luiz Marques; Rasmussen,Lucas Trevizani.
Abstract  Background  Tumor necrosis factor plays a critical role in the pathogenesis of gastric diseases such as gastric cancer, and an abnormal inflammatory response has frequently been observed in dyspeptic patients. Helicobacter pylori infection can induce a gastric mucosal inflammatory response that may be influenced by -308 (G &gt; A) polymorphisms and gene expression of theTNF-α gene.  Methods  One hundred and thirty-four gastric biopsy samples were collected from patients of both genders (61♂ and 73♀, mean age 40.3 ± 24.2 years) with gastric symptoms. The -308 (G &gt; A) polymorphism of TNF-α was characterized using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The expression level was measured using real-time...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: H. pylori; TNF-α Gene expression; Gastric cancer cagA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100355

Molecular detection ofLeishmaniaspp. in road-killed wild mammals in the Central Western area of the State of São Paulo, Brazil
Provedor de dados: 80
Autores: Richini-Pereira,Virginia Bodelão; Marson,Pamela Merlo; Hayasaka,Enio Yoshinori; Victoria,Cassiano; Silva,Rodrigo Costa da; Langoni,Hélio.
Background Road-killed wild animals have been classified as sentinels for detecting such zoonotic pathogens asLeishmaniaspp., offering new opportunities for epidemiological studies of this infection.Methods This study aimed to evaluate the presence ofLeishmaniaspp. andLeishmania chagasiDNA by PCR in tissue samples (lung, liver, spleen, kidney, heart, mesenteric lymph node and adrenal gland) from 70 road-killed wild animals.Results DNA was detected in tissues of oneCavia aperea(Brazilian guinea pig), fiveCerdocyon thous(crab-eating fox), oneDasypus septemcinctus(seven-banded armadillo), twoDidelphis albiventris(white-eared opossum), oneHydrochoerus hydrochoeris(capybara), twoMyrmecophaga tridactyla(giant anteater), oneProcyon cancrivorus(crab-eating...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Road-killed animal; Leishmaniaspp; Leishmania chagasi; PCR; Zoonosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200331

Assessment of genetic relationship between Klebsiella pneumoniae and Klebsiella oxytoca samples isolated from a dental office
Provedor de dados: 80
Autores: Pimenta-Rodrigues,MV; Fusco-Almeida,AM; Bertoni,BW; Pietro,RCLR.
The present study aimed to analyze the genetic similarity between genomic profiles of thirteen Klebsiella oxytoca and seven Klebsiella pneumoniae samples isolated from two different collections carried out in different places of dental offices. Random amplified polymorphic DNA (RAPD) technique and similarity coefficients (calculated by Sorensen-Dice and simple matching) were applied to determine their genetic profile of randomic DNA sequences. The majority of the isolates of K. pneumoniae and K. oxytoca presented similar coefficient values (e" 0.80). Thus, it was possible to identify that strain dissemination occurred mainly via the hands of the surgeon-dentists and, finally, to determine the genetic similarity of the strains from dental office environments.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella oxytoca; Klebsiella pneumoniae; RAPD; Dental office.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400012

Kinetics of rabies antibodies as a strategy for canine active immunization
Provedor de dados: 80
Autores: Babboni,Selene Daniela; Costa,Hení Falcão da; Martorelli,Luzia de Fátima Alves; Kataoka,Ana Paula de Arruda Geraldes; Victoria,Cassiano; Padovani,Carlos Roberto; Modolo,José Rafael.
Background Rabies, a zoonosis found throughout the globe, is caused by a virus of theLyssavirus genus. The disease is transmitted to humans through the inoculation of the virus present in the saliva of infected mammals. Since its prognosis is usually fatal for humans, nationwide public campaigns to vaccinate dogs and cats against rabies aim to break the epidemiological link between the virus and its reservoirs in Brazil.Findings During 12 months we evaluated the active immunity of dogs first vaccinated (booster shot at 30 days after first vaccination) against rabies using the Fuenzalida-Palácios modified vaccine in the urban area of Botucatu city, São Pauto state, Brazil. Of the analyzed dogs, 54.7% maintained protective titers (≥0.5 IU/mL) for 360 days...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Dogs; Vaccination; Revaccination; Annual vaccination campaign; Fuenzalida-Palácios.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200602

Poisoning after ingestion of pufferfish in Brazil: report of 11 cases
Provedor de dados: 80
Autores: Simões,Eneida Márcia de Souza; Mendes,Thelma Marly Abreu; Adão,Angelino; Haddad Junior,and Vidal.
In this brief communication the authors report eleven cases of human poisoning caused by ingestion of pufferfish meat. Three patients (two children and one adult) were seriously affected. The circumstances that precipitated the poisoning are discussed as well as the clinical aspects observed. No deaths were registered and the patients did not present sequelae after the episode.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pufferfish; Marine poisonings; Poisonous animals; Poisonous fish; Tetrodotoxin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200105

Use of antivenoms for the treatment of envenomation by Elapidae snakes in Guinea, Sub-Saharan Africa
Provedor de dados: 80
Autores: Baldé,Mamadou C; Chippaux,Jean-Philippe; Boiro,Mamadou Y; Stock,Roberto P; Massougbodji,Achille.
Background In Guinea Elapids are responsible for 20% of envenomations. The associated case fatality rate (CFR) ranged 15-27%, irrespective of treatment. Results We studied 77 neurotoxic envenomations divided in 3 groups: a set of patients that received only traditional or symptomatic treatments, and two other groups that received either 2 or 4 initial vials of Antivipmyn® Africa renewed as necessary. CFR was 27.3%, 15.4% and 17.6%, respectively. Although antivenom treatment was likely to reduce CFR, it didn’t seem to have an obvious clinical benefit for the patients, suggesting a low treatment efficacy. Mean delay to treatment or clinical stages were not significantly different between the patients who recovered and the patients who died, or between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elapid; Neurotoxins; Treatment; Antivenom; Guinea; Africa.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100304

Experimental Tityus serrulatus scorpion envenomation: age- and sex-related differences in symptoms and mortality in mice
Provedor de dados: 80
Autores: MB,Pucca; EC,Roncolato; LB,Campos; FS,Fernandes; GR,Mendes; TB,Bertolini; FA,Cerni; JE,Barbosa.
Among the various methods for evaluating animal venom toxicity, the calculation of the median lethal dose (LD50) is the most widely used. Although different protocols can be used to calculate the LD50, the source of the venom and the method of extraction, as well as the strain, age, and sex of the animal model employed, should be taken into consideration. The objective of the present study was to evaluate the influence of sex and age on the toxicity of Tityus serrulatus scorpion venom in Swiss mice. Although the symptoms of envenomation were similar in male and female animals, female mice proved to be more resistant to the venom. In females, age had no impact on the susceptibility to scorpion envenomation. Male mice were more sensitive to T. serrulatus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpions; LD50; Stings; Age factors; Gender.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300013

Impact of autologous whole blood administration upon experimental mouse models of acute Trypanosoma cruzi infection
Provedor de dados: 80
Autores: Pavão,Beatriz Philot; Demarque,Kelly Cristina; Batista,Marcos Meuser; Oliveira,Gabriel Melo de; Silva,Cristiane França da; Silva,Francisca Hildemagna Guedes da; Caputo,Luzia Fátima Gonçalves; Cascabulho,Cynthia Machado; Barcinski,Marcello André; Soeiro,Maria de Nazaré Correia.
Abstract  Background: Autologous whole blood (AWB) administration is described as alternative/complementary medical practice widely employed in medical and veterinary therapy against infections, chronic pathologies and neoplasias. Our aim is to investigate in vivo biological effect of AWB using healthy murine models under the course of Trypanosoma cruzi acute infection.  Methods: The first set of studies consisted of injecting different volumes of AWB and saline (SAL) into the posterior region of quadriceps muscle of healthy male Swiss mice under distinct therapeutic schemes evaluating: animal behavior, body and organ weight, hemogram, plasmatic biochemical markers for tissue damage and inflammatory cytokine levels and profile. To assess the impact on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autologous blood; Alternative therapy; Mouse models; Trypanosoma cruzi.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100316

Notes and observations on courtship and mating in Tityus (Atreus) magnimanus Pocock, 1897 (Scorpiones: Buthidae)
Provedor de dados: 80
Autores: Ross,LK.
Courtship and mating behaviors of the scorpion Tityus (Atreus) magnimanus are herein described, consisting of various components that pertain to four distinct behavioral stages. The courtship and mating rituals of Tityus (Atreus) magnimanus are similar to those of other scorpions. Behavioral components are presented in an ethogram to demonstrate their occurrence during mating sequences. The current report is presented as observational data that were acquired during life history studies of this species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpions; Buthidae; Tityus (Atreus) magnimanus; Courtship; Mating; Promenade á deux; Sperm transfer.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100005

A clinical trial protocol to treat massive Africanized honeybee (Apis mellifera) attack with a new apilic antivenom
Provedor de dados: 80
Autores: Barbosa,Alexandre Naime; Boyer,Leslie; Chippaux,Jean-Philippe; Medolago,Natalia Bronzatto; Caramori,Carlos Antonio; Paixão,Ariane Gomes; Poli,João Paulo Vasconcelos; Mendes,Mônica Bannwart; Santos,Lucilene Delazari dos; Ferreira Jr,Rui Seabra; Barraviera,Benedito.
Abstract  Background  Envenomation caused by multiple stings from Africanized honeybees Apis mellifera constitutes a public health problem in the Americas. In 2015, the Brazilian Ministry of Health reported 13,597 accidents (incidence of seven cases per 100,000 inhabitants) with 39 deaths (lethality of 0.25%). The toxins present in the venom, which include melittin and phospholipase A2, cause lesions in diverse organs and systems that may be fatal. As there has been no specific treatment to date, management has been symptomatic and supportive only.  Methods  In order to evaluate the safety and neutralizing capacity of a new apilic antivenom, as well as to confirm its lowest effective dose, a clinical protocol was developed to be applied in a multicenter,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apis mellifera; Bee venom; Toxins; Envenomation; Heterologous serum; Apilic antivenom; Bee antivenom.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100309

Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female rats
Provedor de dados: 80
Autores: Costa,VM; Langoni,H.
The milk of experimentally infected rats was investigated for the presence and possible transmission of Toxoplasma gondii. Wistar (Rattus norvegicus) female rats were divided into three groups and orally inoculated with bradyzoites. Polymerase chain reaction (PCR) was employed to detect the parasite in the milk. Transmission to the offspring was verified by indirect immunofluorescence antibody test (IFAT), modified agglutination test (MAT), bioassay tests and PCR. Rat milk samples were PCR-positive, pups were serum-reactive to T. gondii and tissue samples also presented positive DNA results through PCR.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Parasite in milk; Female rats; PCR; Bioassay.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200016

Rotavirus gastroenteritis in hospital specializing in children with craniofacial malformations
Provedor de dados: 80
Autores: Vieira,N. A.
Tipo: Info:eu-repo/semantics/article
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400021

Pulmonary tuberculosis: hematology, serum biochemistry and the relation with the disease duration
Provedor de dados: 80
Autores: Oliva,V. M.; Cezário,G. A. G.; Cocato,R. A.; Marcondes-Machado,J.
The aim of the present study was to analyze the relationship between hematological and biochemical parameters and tuberculosis process activity time according to clinical complaint duration. It was a retrospective study analyzing medical records from 80 pulmonary tuberculosis patients at Botucatu Medical School University Hospital (Botucatu, São Paulo State, Brazil), who were divided into 2 groups according to clinical complaint duration: Group 1 (G1) - up to three months; Group 2 (G2) - over three months. Parameters included: age, gender, bacilloscopy, erythrocyte sedimentation rate (ESR), platelet count, alpha1-globulin, alpha2-globulin, gamma globulin, mucoprotein, alpha1-acid glycoprotein values, and the presence of risk factors such as smoking,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Pulmonary tuberculosis; Smoking; Hematolological and biochemical parameters; Erythrocyte sedimentation rate.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100006

Hemolytic toxin from the soft coral Sarcophyton trocheliophorum: isolation and physiological characterization
Provedor de dados: 80
Autores: Karthikayalu,S; Rama,V; Kirubagaran,R; Venkatesan,R.
The unifying characteristic of cnidarians is the production of protein and polypeptide toxins. The present study describes the identification of a hemolytic toxin from the soft coral Sarcophyton trocheliophorum. The crude extract was highly cytotoxic (EC50 = 50 ng/mL) against human erythrocytes. It was also tested for hemolytic activity by the blood agar plate method, resulting in a hemolytic halo of 12 mm with 50 µg of protein. The stability of the venom under different physiological conditions was analyzed. The venom hemolytic activity was augmented by alkaline and neutral pH whereas it was reduced in acidic pH. The activity was stable up to 60º C. The hemolytic activity was completely abolished by the addition of serum and reduced significantly during...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sarcophyton trocheliophorum; Cnidarian toxin; Cytolysin.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100011

Seroprevalence of Chagas disease in dwellers of the XI Regional Health Administration Districts, São Paulo State, Brazil
Provedor de dados: 80
Autores: Godoy,I.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100018

Immunophenotypic characterization of patients with American cutaneous leishmaniasis prior to and after treatment in Pernambuco, Brazil
Provedor de dados: 80
Autores: Almeida,AF; Castro,MCAB; Oliveira,AP; Souza,MA; Pereira,VRA.
Leishmania infections induce a specific activation of host immunological response, particularly characterized by T cell expansion. Studies indicate the importance of the balance between CD4+ and CD8+ T cells, in which the first ones would have their number reduced during the healing process. Meanwhile, CD25+ T cells have been associated with the suppression of the immune response. Since the immune response has an essential role in both healing and progression of diseases, this study aimed to identify the percentage of CD3+, CD4+, CD8+, CD16+ and CD25+ T cells in the peripheral blood of patients afflicted with American cutaneous leishmaniasis (ACL) - before and after treatment - and healthy controls. Peripheral blood was collected and transferred to...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: American tegumentary leishmaniasis; Cellular immune response; Flow cytometry; Leishmania (Viannia) braziliensis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200016

A spider derived peptide, PnPP-19, induces central antinociception mediated by opioid and cannabinoid systems
Provedor de dados: 80
Autores: Pacheco,Daniela da Fonseca; Freitas,Ana Cristina Nogueira; Pimenta,Adriano Monteiro C.; Duarte,Igor Dimitri Gama; Lima,Maria Elena de.
Abstract  Background  Some peptides purified from the venom of the spider Phoneutria nigriventer have been identified as potential sources of drugs for pain treatment. In this study, we characterized the antinociceptive effect of the peptide PnPP-19 on the central nervous system and investigated the possible involvement of opioid and cannabinoid systems in its action mechanism.  Methods  Nociceptive threshold to thermal stimulation was measured according to the tail-flick test in Swiss mice. All drugs were administered by the intracerebroventricular route.  Results  PnPP-19 induced central antinociception in mice in the doses of 0.5 and 1 μg. The non-selective opioid receptor antagonist naloxone (2.5 and 5 μg), μ-opioid receptor antagonist clocinnamox (2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peptide PnPP-19; Central antinociception; Phoneutria nigriventer; Μ-opioid receptor; Δ-opioid receptor; CB1 receptor; CB2 receptor.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100325

Errata
Provedor de dados: 80
Tipo: Info:eu-repo/semantics/other
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200009

In vivo evaluation of homeostatic effects of Echis carinatus snake venom in Iran
Provedor de dados: 80
Autores: Salmanizadeh,Hossein; Babaie,Mahdi; Zolfagharian,Hossein.
Background The venom of the family Viperidae, including the saw-scaled viper, is rich in serine proteinases and metalloproteinases, which affect the nervous system, complementary system, blood coagulation, platelet aggregation and blood pressure. One of the most prominent effects of the snake venom of Echis carinatus (Ec) is its coagulation activity, used for killing prey. Materials and methods Subfractions F1A and F1B were isolated from Ec crude venom by a combination of gel chromatography (Sephadex G-75) and ion exchange chromatography on a DEAE-Sepharose (DE-52). These subfractions were then intravenously (IV) injected into NIH male mice. Blood samples were taken before and after the administration of these subfractions. Times for prothrombin, partial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Procoagulant activity; Blood coagulation; Echis carinatus; Chromatography; LD50.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100302

Preliminary studies with a neurotoxin obtained from Bungarus caeruleus venom
Provedor de dados: 80
Autores: Mirajkar,K. K.; More,S.; Gadag,J. R.
The neurotoxin purified from the venom of Bungarus caeruleus causes a neuromuscular blockade on acetylcholine-induced muscle twitch response in isolated frog rectus abdominis muscle preparation. Neuromuscular blockade produced by d-tubocurarine on acetylcholine-induced muscle twitch response in an isolated frog rectus abdominis muscle preparation was reversed to normal muscle twitch response in presence of neostigmine. Whereas the purified neurotoxin produced an irreversible neuromuscular blockade in presence of the same strength of neostigmine. As it is already known, botulinum toxin, which also brings about neuromuscular blockade, is effectively used as a drug in the treatment of painful movement disorders. Since the purified toxin also causes paralysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuromuscular block; Bungarus caeruleus.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100007

Determination of in vivo toxicity and in vitro cytotoxicity of venom from the Cypriot blunt-nosed viper Macrovipera lebetina lebetina and antivenom production
Provedor de dados: 80
Autores: Nalbantsoy,A; Karabay-Yavasoglu,NU; Sayim,F; Deliloglu-Gurhan,I; Gocmen,B; Arikan,H; Yildiz,MZ.
The venomous Levantine viper, Macrovipera lebetina lebetina is endemic to Cyprus. The objective of this study was to investigate in vitro cytotoxicity, in vivo lethality, and antivenom production followed by a re-immunization schedule in mice against Macrovipera lebetina lebetina venom. The LD50 value was estimated as 7.58 mg/kg within 24 hours by different venom doses administrated intraperitoneally in mice. Freund's complete and incomplete adjuvants were used for first and second immunization of mice in antivenom production. A cell-based assay was performed to determine the effects of Macrovipera lebetina lebetina venom and antivenom neutralizing potency on L929 cell viability. The snake venom toxicity and cytotoxicity were examined and comparison of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Macrovipera lebetina lebetina; LD50; Lethality; Snake venom; Antivenom.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200011

Epidemiological profile of snakebites in Rio de Janeiro, Brazil, 2001-2006
Provedor de dados: 80
Autores: Machado,C; Bochner,R; Fiszon,JT.
All snakebites registered in Rio de Janeiro state, Brazil, between 2001 and 2006 were analyzed. Of these, the snake species involved was identified in 2,431 cases, most of which were caused by Bothrops (2,347). Most victims were male (78.4%) and the age group most frequently attacked was between 20 and 39 years (38.4%). As for severity, 1,118 (45.9%) were classified as minor, 748 (30.7%) as moderate, and 209 (8.6%) as severe. Progression towards complete cure occurred in 1,567 patients; 39 had sequelae; three died; and clinical outcomes were not monitored in 822 cases. The time between bite and first medical attention was less than three hours in 74.6% of cases. Most occurred in municipalities with vast areas of preserved Atlantic Forest: Angra dos Reis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snakebites; Envenomation; Snake bite epidemiology; Information systems.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200012

Experimental envenomation with Crotalus durissus terrificus venom in dogs treated with antiophidic serum - part I: clinical evaluation, hematology and myelogram
Provedor de dados: 80
Autores: Nogueira,R. M. B.; Sakate,M.; Sangiorgio,F.; Laposy,C. B.; Melero,M.
The present study aimed at evaluating clinical and laboratory aspects during experimental envenomation by Crotalus durissus terrificus in dogs treated with antiophidic serum. Twenty-one dogs were divided into three groups of seven animals each. Group I received 1mg/kg venom (sc); Group II received 1mg/kg venom (sc), 50mg antiophidic serum (iv), and fluid therapy including 0.9% NaCl solution (iv); and Group III received 1mg/kg venom (sc), 50mg antiophidic serum (iv), and fluid therapy including 0.9% NaCl solution containing sodium bicarbonate diluted to the dose of 4mEq/kg. The clinical signs of ataxia, sedation, flaccid paralysis, mydriasis, eyeball paralysis, mandible ptosis, sialorrhea, vomiting and diarrhea observed in the dogs were very similar to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durissus terrificus; Dogs; Clinical aspects; Laboratory aspects.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400010

The epidemiology of scorpion stings in tropical areas of Kermanshah province, Iran, during 2008 and 2009
Provedor de dados: 80
Autores: Khatony,Alireza; Abdi,Alireza; Fatahpour,Tahereh; Towhidi,Farhad.
Abstract  Background  Scorpion stings are an acute health problem in tropical regions. Awareness of this problem is fundamental for establishing preventive interventions, thus prompting the present study to determine the scorpion-sting incidence in tropical areas of Kermanshah province during 2008 and 2009.  Methods  In a retrospective study, all records related to scorpion sting patients from the health centers of tropical areas of Kermanshah were studied by a census and checklist. Data were analyzed by the software SPSS-16 using descriptive and inferential tests.  Results  The incidence of scorpion stings was 334.37/100,000 inhabitants in 2008 and 339.07/100000 in 2009. Mean and standard deviation of age were 30.55 ± 16.99. Scorpion stings were more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion stings; Epidemiology; Tropical climate; Kermanshah; Incidence.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100349

Structural determinants of the hyperalgesic activity of myotoxic Lys49-phospholipase A2
Provedor de dados: 80
Autores: Zambelli,Vanessa Olzon; Chioato,Lucimara; Gutierrez,Vanessa Pacciari; Ward,Richard John; Cury,Yara.
Abstract  Background  Bothropstoxin-I (BthTx-I) is a Lys49-phospholipase A2 (Lys49-PLA2) from the venom of Bothrops jararacussu, which despite of the lack of catalytic activity induces myotoxicity, inflammation and pain. The C-terminal region of the Lys49-PLA2s is important for these effects; however, the amino acid residues that determine hyperalgesia and edema are unknown. The aim of this study was to characterize the structural determinants for the Lys49-PLA2-induced nociception and inflammation.  Methods  Scanning alanine mutagenesis in the active-site and C-terminal regions of BthTx-I has been used to study the structural determinants of toxin activities. The R118A mutant was employed as this substitution decreases PLA2 myotoxicity. In addition, K115A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lys49-Phospholipase A2; Hyperalgesia; Site-directed mutagenesis; Myotoxic effect; Edema; Membrane damage.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100304

Borderline leprosy: in situ and cytokine profile in supernatant of mononuclear of cell culture
Provedor de dados: 80
Autores: Venturini,J.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200018

Evaluation of the antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus isolated from human infections
Provedor de dados: 80
Autores: Domingues,Andréa.
Tipo: Info:eu-repo/semantics/article
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300014

Is there a non-present teaching proposal for Methodology in health?
Provedor de dados: 80
Autores: Ferreira Junior,Rui Seabra.
Tipo: Info:eu-repo/semantics/other
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200001

Renal and vascular effects of Crotalus durissus cumanensis venom and its crotoxin fraction
Provedor de dados: 80
Autores: Pereira,TP; Bezerra de Menezes,RRPP; Torres,AFC; Brito,TS; Batista-Lima,FJ; Vinhote,JFC; Sousa,DF; Ximenes,RM; Toyama,MH; Diz Filho,EBS; Magalhães,PJC; Monteiro,HSA; Martins,AMC.
In this study, we evaluated the actions of Crotalus durissus cumanensis venom (CDCmV), and its crotoxin (Crtx) fraction, on renal and vascular functions in Wistar rats. In isolated perfused kidneys, CDCmV (10 µg/mL) significantly increased the perfusion pressure (PP) from 110.7 ± 2.4 to 125.3 ± 2.8 mmHg after 30 minutes. This effect was accompanied by an increased renal vascular resistance (RVR) from 5.4 ± 0.1 to 6.2 ± 0.2 mmHg/mL.g-1.min-1. We observed decreases in urinary flow (UF) from 0.13 ± 0.01 to 0.05 ± 001 mL.g-1.min-1 and glomerular filtration rate (GFR) from 0.66 ± 0.06 to 0.18 ± 0.02 mL.g-1.min-1. Crtx did not change PP or RVR, but diminished GFR (from 0.65 ± 0.05 to 0.26 ± 003 mL.g-1.min-1) and UF (from 0.11 ± 0.008 to 0.09 ± 0.008...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney; Vascular injuries; Crotalus durissus cumanensis; Crotoxin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300014

Epidemiological and clinical characteristics of scorpion stings in children in fez, Morocco
Provedor de dados: 80
Autores: Abourazzak,S; Achour,S; El Arqam,L; Atmani,S; Chaouki,S; Semlali,I; Soulaymani Bencheikh,R; Bouharrou,A; Hida,M.
Scorpion stings are a public health problem in Morocco, especially among children, who experience the most severe cases. Epidemiological and clinical findings on scorpion stings in Fez, Morocco, were evaluated in this investigation. Of 163 cases that required medical attention, 62.6% were male children. The mean age of patients was 4.8 ± 3.4 years. The mean time between stings and first medical attention was 3.36 ± 2.5 hours. Almost all cases occurred in the summer (94%) and extremities represented the most frequent sting sites (86.5%). Local pain, hyperemia, scarification, vomiting, sweating, restlessness, tachycardia and tachypnea were the observed clinical symptoms. Regarding severity, 55.2% of patients belonged to class III, followed by class II...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion sting; Clinical symptoms; Envenomation; Treatment; Children; Morocco.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200008

Toxinology and Brazil: a close connection
Provedor de dados: 80
Autores: Ferreira Jr,RS.
Tipo: Info:eu-repo/semantics/other
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300001

Esterase electrophoresis of Clostridium perfringens bovine strains
Provedor de dados: 80
Autores: Baldassi,L.; Barbosa,M.L.; Bach,E.E.; Iaria,S.T.
Eighty-nine of 144 isolates of Clostridium perfringens obtained from 187 samples of 71 bovine in several Brazilian states were submitted to esterase electrophoresis for typing. Mobilites electrophoresis, as parameter, were settled down by isolates from ATCC pattern of types A, B, C, and D. Of the 89 isolates, 43 (48.3%) were characterized as electrophoretic type A, 20 (22.5%) as D, 18 (20.2%) as C, and 3 (3.4%) as B. Five (5.6%) isolates did not identity with any type. Similarly, esterase electrophoresis enabled the typing of 94.4 % of the isolates, demonstrating to be an appropriate method for animal sample analyses.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Bovine; Electrophoresis; Esterase.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200009

Impact factor: the purpose of its use
Provedor de dados: 80
Autores: Santos,Jose Claudio.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100001

Chagas' disease and Duffy antigen/receptor for chemokine (DARC): a mini-review
Provedor de dados: 80
Autores: Oliveira,AP; Mattos,LC; Cavasini,CE.
Duffy gene (FY) codifies the transmembrane glycoprotein Duffy (gp-Fy) of 35 to 43 kDa which is moderately immunogenic. This glycoprotein is polymorphic, and constitutes the antigens of the Duffy histo-blood system which were designated receptors for chemokines and denominated DARC (Duffy antigen/receptor for chemokine). This receptor has an important role in the regulation of chemokine levels in the circulation, as it binds and adsorbs them on the surface of red cells as a reservoir. It plays a "sink" role, which can contribute to homeostasis by removing inflammatory chemokines from circulation as well as maintaining them in plasmatic levels. Chronic Chagas' cardiopathy (CCC) is the most frequent form of the disease. It is an inflammatory disease, in which...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DARC antigen; Duffy blood-group system; Chagas' disease; Chagas' cardiomyopathy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300005

Laboratory evaluation of young ovines inoculated with natural or 60co-irradiated Crotalus durissus terrificus venom during hyperimmunization process
Provedor de dados: 80
Autores: Ferreira Junior,R. S.; Nascimento,N.; Couto,R.; Alves,J. B.; Meira,D. A.; Barraviera,B.
Laboratory profile of young ovines was studied in order to evaluate and compare their antiserum production from natural and Cobalt-60 irradiated Crotalus durissus terrificus (C.d.t.) venoms. The parameters analyzed included complete blood count, and urea, creatinine, aspartate aminotransferase, total proteins, albumin and globulin serum measurements. Three groups of six animals each were used. Group 1 (G1) received natural C.d.t. venom; Group 2 (G2) received irradiated C.d.t. venom; and Group 3 (G3) was used as control and did not receive venom, only adjuvants, using seven venom inoculations. During the experimental period, animals were fortnightly weighed. According to clinical and weight evaluation, sheep in post-weaning phase showed no changes in their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durissus terrificus; Hyperimmunization; Ovines; Antivenom; Irradiation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400009

The Brazilian Society of Toxinology (SBTx) comes of age
Provedor de dados: 80
Autores: Lima,Maria Elena de.
Tipo: Info:eu-repo/semantics/other
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100001

Clinical and hematological alterations in dogs during experimental envenomation with Crotalus durissus terrificus venom and treated with antiophidic serum
Provedor de dados: 80
Autores: Nogueira,R. M. B.; Sakate,M.
The present work aimed to evaluate the clinical and hematological aspects during experimental envenomation by Crotalus durissus terrificus in dogs treated with different antiophidic serum doses. Sixteen dogs were divided into two groups of eight animals each. Group I received 1mg/kg venom subcutaneously and 30mg antiophidic serum intravenously; Group II received 1mg/kg venom subcutaneously and 60mg antiophidic serum intravenously. In the clinical evaluation, we observed ataxia, moderate sedation, dilated pupils, sialorrhea, flaccid paralysis of mandibular muscles, and discreet edema at the site of venom inoculation. Evaluating red and white blood cells, we observed a decrease of hemoglobins, globular volume and erythrocytes, and an increase of plasmatic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durissus terrificus; Dog; Clinical aspects; Hematological aspects; Venom.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200010

Characterization, purification and phylogenetic analysis of a cytolysin from the sea anemone Heteractis magnifica of the Indian Ocean
Provedor de dados: 80
Autores: Karthikayalu,S; Rama,V; Kirubagaran,R; Venkatesan,R.
It is well established that sea anemones comprise a rich source of cytolytic toxins. The present study reports the isolation and characterization of a cytolysin obtained from the sea anemone Heteractis magnifica collected in the Andaman Islands of the Indian Ocean. The crude extract was screened for hemolytic activity by a blood agar plate method and a 6-mm zone of clearance was observed after incubation. The hemolytic property of the crude extract, tested by the microtiter plate method, revealed positive results at concentrations as low as 120 ng/mL. Furthermore, it was favored by alkaline pH and was stable up to 60°C. On the other hand, the hemolytic effect was abolished by the addition of human serum. Purification steps involved ammonium sulfate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heteractis magnifica; Marine toxin; Hemolysin; Cytolysin.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200006

Differential susceptibilities of human lung, breast and skin cancer cell lines to killing by five sea anemone venoms
Provedor de dados: 80
Autores: Ramezanpour,M; Burke da Silva,K; Sanderson,BJ.
Although sea anemones are well known for being rich sources of toxins, including cytolysins and neurotoxins, their venoms and toxins have been poorly studied. In the present study, the venoms from five sea anemones (Heteractis crispa, Heteractis magnifica, Heteractis malu, Cryptodendrum adhaesivum and Entacmaea quadricolor) were obtained by the milking technique, and the potential of these venoms to kill cancer cells was tested on three cell lines (A549 lung cancer, T47D breast cancer and A431 skin cancer). The total protein level in the crude extract was determined by the bicinchoninic acid (BCA) protein assay. The cytotoxicity on different cell lines was assayed using the 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay which...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sea anemone venoms; Cytolysin; Cancer cell line.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200005

Activation of phagocytes from peripheral human blood by Paracoccidioides brasiliensis and enteropathogenic Escherichia coli. Production of superoxide anion and modulation by melatonin hormone
Provedor de dados: 80
Autores: França,E. L.
Tipo: Info:eu-repo/semantics/article
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400023

Crotalus durissus terrificus venom as a source of antitumoral agents
Provedor de dados: 80
Autores: Soares,MA; Pujatti,PB; Fortes-Dias,CL; Antonelli,L; Santos,RG.
The basic knowledge on neoplasms is increasing quickly; however, few advances have been achieved in clinical therapy against tumors. For this reason, the development of alternative drugs is relevant in the attempt to improve prognosis and to increase patients' survival. Snake venoms are natural sources of bioactive substances with therapeutic potential. The objective of this work was to identify and characterize the antitumoral effect of Crotalus durissus terrificus venom (CV) and its polypeptide, crotoxin, on benign and malignant tumors, respectively, pituitary adenoma and glioblastoma. The results demonstrated that CV possess a powerful antitumoral effect on benign (pituitary adenoma) and malignant (glioblastoma multiforme) tumors with IC50 values of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durissus terrificus venom; Crotoxin; Antitumoral effect; Pituitary adenoma; Glioblastoma multiforme.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300015

Scorpionism by Hemiscorpius spp. in Iran: a review
Provedor de dados: 80
Autores: Dehghani,Rouhullah; Kamiabi,Fatemeh; Mohammadi,Malihe.
Abstract Scorpions are distributed throughout Iran and the genus Hemiscorpius is particularly important in this region. Hemiscorpius lepturus is the most significant species within the genus in the country. Since scorpionism provoked by Hemiscorpius comprises a medical emergency, the present study is focused on this important issue. In order to perform the present work, a review of the medical and health-related literature was carried out in several databases. The current findings indicate that six species of Hemiscorpius are found in 15 states of Iran, mainly in the south and southwest. Deaths caused by stings were reported only for two species. The morphological characteristics and geographical distribution of H. lepturus in Iran, its venom and the toxic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemiscorpius; Scorpionism; Scorpion venom; Emergency; Epidemiology.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100204

Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth
Provedor de dados: 80
Autores: Della Torre,A; Albuquerque,LBL; Farrapo,NM; Oshima-Franco,Y; Santos,MG; Tavares,RVS; Rodas,ACD; Dal Belo,CA; Cardoso,CRP; Varanda,EA; Groppo,FC; Lopes,PS.
Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins). The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test) and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9) and without (-S9) metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ames test; Micronucleus test; Mutagenicity; Plant extract; Plathymenia reticulata.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200010

The effects of a sodium and a calcium channel blocker on lethality of mice injected with the yellow scorpion (Leiurus quinquestriatus) venom
Provedor de dados: 80
Autores: Al-Shanawani,A. R.; Fatani,A. J.; El-Sayed,F. H.
Scorpion venom toxins generally produce similar effects by mainly acting on sodium channels, and to a lesser extent, on potassium, calcium, and chloride channels. This leads to increased release of neurotransmitters and mediators, resulting in a cascade of pathological events, involving the central nervous system, the autonomic nervous system, the cardiovascular and the respiratory system, eventually leading to death. The objective of this paper was to discover whether a sodium channel blocker, lidocaine, or a calcium channel blocker, verapamil, would prolong the survival of mice injected with the venom from the common yellow scorpion Leiurus quinquestriatus quinquestriatus (LQQ). For this purpose, mice were divided into 2 groups, each injected with a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leiurus quinquestriatus quinquestriatus scorpion venom neurotoxins Na+ channels Ca2+ channels lidocaine; Verapamil mice lethality.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200008

Frequency of spermatophore production and regeneration in the males of Tityus (Atreus) magnimanus Pocock, 1897 (Scorpiones, Buthidae)
Provedor de dados: 80
Autores: Ross,LK.
The frequency of spermatophore production and the amount of time between matings for laboratory-reared males of Tityus (Atreus) magnimanus to regenerate spermatophores were examined. Males attain sexual maturity at the fifth or sixth instar (after a period of 137 to 155 days) and can produce initial spermatophores shortly after maturation. After mating, males can regenerate spermatophores within a single 24-hour period and remate. The present contribution represents the first report on an aspect of reproductive biology in this species and is part of a continuing study of the life history and post-embryonic development of Tityus (Atreus) magnimanus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpions; Buthidae; Tityus (Atreus) magnimanus; Courtship; Mating; Spermatophores; Sperm transfer.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100014

Circadian pattern of Bothrops moojeni in captivity (Serpentes: Viperidae)
Provedor de dados: 80
Autores: Stuginski,DR; Sant'Anna,SS; Fernandes,W; Abe,AS.
Members of the subfamily Crotalinae are considered to be essentially nocturnal and most of the data about these snakes have been collected from the field. Information on how nutritional status affects the movement rate and activity patterns is a key point to elucidating the ecophysiology of snakes. In this study, we distributed 28 lancehead Bothrops moojeni into three groups under distinct feeding regimens after a month of fasting. Groups were divided as follows: ingestion of meals weighing (A) 40%, (B) 20%, or (C) 10% of the snake body mass. Groups were monitored for five days before and after food intake and the activity periods and movement rates were recorded. Our results show that B. moojeni is prevalently nocturnal, and the activity peak occurs in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops moojeni; Snake activity; Post-prandial; Circadian rhythms; Captivity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100012

Injuries by marine and freshwater stingrays: history, clinical aspects of the envenomations and current status of a neglected problem in Brazil
Provedor de dados: 80
Autores: Junior,Vidal Haddad; Cardoso,João Luiz Costa; Neto,Domingos Garrone.
Stingrays are a group of rays – cartilaginous fish related to sharks – that have whiplike tails with barbed, usually venomous spines and are found around the world, especially the marine species. Despite recent reports of accidents involving these fish, they are not aggressive, reacting only when stepped on or improperly handled. Injuries by stingrays are seldom mentioned by historians, although they have always been present in riverine communities of inland waters and in South American coasts. Indeed, envenomations by stingrays are quite common in freshwater and marine fishing communities. Although having high morbidity, such injuries are neglected because they have low lethality and usually occur in remote areas, which favor the use of folk remedies. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sting; Freshwater stingrays; Marine stingrays; Venomous animals; Venomous fish.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100202

Molecular techniques for the study and diagnosis of parasite infection
Provedor de dados: 80
Autores: Tavares,RG; Staggemeier,R; Borges,ALP; Rodrigues,MT; Castelan,LA; Vasconcelos,J; Anschau,ME; Spalding,SM.
In parasitology, routine laboratory diagnosis involves conventional methods, such as optical microscopy, used for the morphological identification of parasites. Currently, molecular biology techniques are increasingly used to diagnose parasite structures in order to enhance the identification and characterization of parasites. The objective of the present study was to review the main current and new diagnostic techniques for confirmation of parasite infections, namely: polymerase chain reaction (PCR), real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), Luminex xMAP, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and restriction fragment length polymorphism (RFLP), in addition...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parasite infection; Diagnosis; Molecular techniques; Molecular epidemiology.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300003

Snake venom derived fibrin adhesive in dog uterine healing: biomechanical study
Provedor de dados: 80
Autores: Moraes,J. R. E.; Camplesi,A. C.; Correia,P. H. A.; Shimano,A. C.; Ferraro,G. C.; Moraes,F. R.
Healing is a complex process with many interfering factors. The objective of this work was to evaluate regeneration strength of non-pregnant adult dog uterus when snake venom derived fibrin adhesive is used to reinforce hysterorrhaphy. Maximum limit and rigidity were analyzed. Twenty uterine horns from 10 dogs were hysterotomized and distributed into 2 equal groups. Hysterorrhaphy was performed using the Shimieden-Cushing double layer suture. In one group, animals received snake venom derived fibrin adhesive as reinforcement. Although neither variable was significantly different, our results showed higher rigidity values in the adhesive group. This can be attributed to the adhesive’s effect on organ elasticity or to more granulation tissue formed in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomechanical properties; Healing; Dog uterus; Fibrin adhesive; Snake venom.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200005

Immune response to Rhodococcus equi infection in high and low antibody-producer mice (selection IV-A)
Provedor de dados: 80
Autores: Pedrini,S. C. B.
Tipo: Info:eu-repo/semantics/article
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400024

Ethical considerations of artificial insemination
Provedor de dados: 80
Autores: Avila,RE; Samar,ME.
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400002

Characteristics of hemolytic activity induced by skin secretions of the frog Kaloula pulchra hainana
Provedor de dados: 80
Autores: Wei,Shuangshuang; Chi,Tingting; Meng,Aiyun; Chen,Congwei; An,Tianchen; Wang,Manchuriga; Zhang,Yingxia.
Background The hemolytic activity of skin secretions obtained by stimulating the frog Kaloula pulchra hainana with diethyl ether was tested using human, cattle, rabbit, and chicken erythrocytes. The skin secretions had a significant concentration-dependent hemolytic effect on erythrocytes. The hemolytic activity of the skin secretions was studied in the presence of osmotic protectants (polyethylene glycols and carbohydrates), cations (Mg2+, Ca2+, Ba2+, Cu2+, and K+), or antioxidants (ascorbic acid, reduced glutathione, and cysteine). Results Depending on their molecular mass, osmotic protectants effectively inhibited hemolysis. The inhibition of skin hemolysis was observed after treatment with polyethylene glycols (1000, 3400, and 6000 Da). Among divalent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amphibian; Skin secretions; Hemolysis; Pore-forming.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100306

Intravitreal injection of the synthetic peptide LyeTx I b, derived from a spider toxin, into the rabbit eye is safe and prevents neovascularization in a chorioallantoic membrane model
Provedor de dados: 80
Autores: Silva,Flavia Rodrigues da; Paiva,Mayara Rodrigues Brandão de; Dourado,Lays Fernanda Nunes; Silva,Rummenigge Oliveira; Silva,Carolina Nunes da; Costa,Bruna Lopes da; Toledo,Cibele Rodrigues; Lima,Maria Elena de; Silva-Cunha,Armando da.
Abstract  Background: The great diversity of molecules found in spider venoms include amino acids, polyamines, proteins and peptides, among others. Some of these compounds can interact with different neuronal receptors and ion channels including those present in the ocular system. To study potential toxicity and safety of intravitreal injection in rabbits of LyeTx I b, a synthetic peptide derived from the toxin LyeTx I found in venom from the spider Lycosa eritrognatha and to evaluate the angiogenic activity on a CAM model.  Methods: ARPE-19 cells were treated with LyeTx I b (0.36; 0.54; 0.72; 2.89; 4.34 or 9.06 μM). In this study, New Zealand rabbits were used. LyeTx I b (2.89 μM) labeled with FITC dissolved in PBS, or only PBS, were injected into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lycosa eritrognatha; LyeTx I b; Intravitreal injection; Retinal diseases; Toxicity; Retinal neovascularization.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100322

Antibacterial activity of Rhynocoris marginatus (Fab.) and Catamirus brevipennis (Servile) (Hemiptera: reduviidae) venomS against human pathogens
Provedor de dados: 80
Autores: Sahayaraj,K.; Borgio,J. F.; Muthukumar,S.; Anandh,G. P.
The reduviid predators Rhynocoris marginatus (Fab.) and Catamirus brevipennis (Servile) use their venoms to paralyze their preys. We detected the antibacterial activity of R. marginatus and C. brevipennis venoms against seven Gram-negative and four Gram-positive bacteria by using the disc diffusion method. Rhynocoris marginatus venom exhibited antibacterial activity against four Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Salmonella typhimurium) and one Gram-positive (Streptococcus pyogenes). Catamirus brevipennis venom showed antibacterial activity against six Gram-negative (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus vulgaris, and Salmonella typhimurium) and three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibacterial activity; Reduviid saliva; Protein content; Peptides.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300011

Brain and lung cryptococcoma and concurrent corynebacterium pseudotuberculosis infection in a goat: a case report
Provedor de dados: 80
Autores: Luvizotto,MCR; Carreira,VS; Ferrari,HF; Ribeiro,D; Vallim,MA; Azevedo,V; Cardoso,TC.
A four-year-old male goat with a history of neurological disorder was euthanized. It presented uncommon nodules in the brain and lungs associated with multiple abscesses, predominantly in the spleen and liver. Histological examination of brain and lung sections revealed yeast forms confirmed to be Cryptococcus gattii after a combination of isolation and polymerase chain reaction (PCR) procedures. Moreover, Corynebacterium pseudotuberculosis infection was diagnosed by PCR of samples from the lung, spleen and liver. The present report highlights the rare concurrent infection of C. gatti and C. pseudotuberculosis in an adult goat from São Paulo state, Brazil, and indicates the necessity of surveillance in the treatment of goats with atypical pulmonary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryptococcus gattii; Corynebacterium pseudotuberculosis; Meningoencephalitis; Goat.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300015

Characterization of TH profile through detection of serum cytokine levels in patients with tuberculosis associated or not with HIV infection
Provedor de dados: 80
Autores: Silva,S. M. U. R.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100019

Detection of Toxoplasma gondii DNA in sera samples of mice experimentally infected
Provedor de dados: 80
Autores: Langoni,H.; Das Dores,C. B.; Silva,R. C.; Pezerico,S. B.; Castro,A. P. B.; Da Silva,A. V.
Detection of Toxoplasma gondii (T. gondii) DNA in blood can help to diagnose the disease in its acute phase; however, it must be considered that hemoglobin, present in blood, can inhibit polymerase activity, making impracticable the detection of DNA in samples. Mice were experimentally infected via oral route with ME49 and BTU2 strains cysts and RH strain tachyzoites; polymerase chain reaction was used to detect T. gondii DNA in mice sera 18, 24, 48, 96, and 192 hours post infection (PI). Toxoplama gondii DNA was detected in only one animal infected with BTU2 strain, genotype III (isolated from a dog with neurological signs) 18 hours PI. The agent's DNA was not detected in any sample of the other experimental groups. New studies must be carried out to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasmosis; PCR; Serum; Experimental infection; Mice.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200004

Snakebites in Paraíba, Brazil
Provedor de dados: 80
Autores: Albuquerque,H. N.; Fernandes,A.; Albuquerque,I. C. S.
The epidemiological data on snakebites in the state of Paraíba, Brazil, is scarce. This accounts for the difficulties in helping the victims. Hence, to obtain the clinical and epidemic profile of the snakebite accidents in Paraíba, we studied the number of cases recorded in this state between January 1995 and December 2000. The data was collected from these sources: Center for Toxicological Assistance (Centro de Assistência Toxicológica - CEATOX) and State Secretariat of Health (Secretaria Estadual de Saude), João Pessoa; 3rd Regional Health Center (3º Núcleo Regional de Saúde - NRS) and the Division of Epidemiological Vigilance of the Municipal Secretariat of Health (Divisão de Vigilância Epidemiológica da Secretaria Municipal de Saúde - DVE/SMS), Campina...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ophidic accident; Paraíba; Public health care; Environment.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300003

A glycoprotein with anti-inflammatory properties secreted by an Aspergillus nidulans modified strain
Provedor de dados: 80
Autores: Queiroz,J. C. F.; Teixeira,C. F. P.; Lebrun,I.; Spencer,P. J.; Zucchi,T. M. A. D.
Total RNA from lipopolysaccharide (LPS)-stimulated rat macrophages used to treat protoplasts from an Aspergillus nidulans strain originated the RT2 regenerated strain, whose culture supernatant showed anti-inflammatory activity in Wistar rats. The protein fraction presenting such anti-inflammatory activity was purified and biochemically identified. The screening of the fraction responsible for such anti-inflammatory property was performed by evaluating the inhibition of carrageenan-induced paw edema in male Swiss mice. Biochemical analyses of the anti-inflammatory protein used chromatography, carbohydrates quantification of the protein sample, amino acids content analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Total sugar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus nidulans; Protein purification and characterization; Glycoprotein; Anti-inflammatory activity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200010

Comparison of infection by Brucella spp. in free-ranging and captive wild animals from São Paulo State, Brazil
Provedor de dados: 80
Autores: Antunes,JMAP; Machado,GP; Costa,LF; Fornazari,F; Cipriano,JRB; Appolinário,CM; Allendorf,SD; Bagagli,E; Teixeira,CR; Megid,J.
The aim of the current study was to evaluate the infection rate by Brucella spp. in wild and in captive animals. Serum samples from 121 animals (94 free-ranging and 27 captive) of different mammal species were evaluated. Sera were submitted to rose Bengal test (RBT) for screening and serum agglutination tests (SAT) and 2-mercaptoethanol test (2-ME) for confirmatory results. Nine animals (five free-ranging and four captive) tested positive in RBT, but negative in the confirmatory tests. Several domestic animal diseases that have control programs are not focused on wild reservoirs, such as brucellosis in Brazil. The study of new reservoirs in wildlife is essential to prevent emerging diseases.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brucellosis; Wildlife; Zoonosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400017

São Paulo School of Advanced Sciences on Vaccines: an overview
Provedor de dados: 80
Autores: Sorgi,Sara; Bonezi,Vivian; Dominguez,Mariana R.; Gimenez,Alba Marina; Dobrescu,Irina; Boscardin,Silvia; Nakaya,Helder I.; Bargieri,Daniel Y.; Soares,Irene S.; Silveira,Eduardo L. V.
Abstract Two years ago, we held an exciting event entitled the São Paulo School of Advanced Sciences on Vaccines (SPSASV). Sixty-eight Ph.D. students, postdoctoral fellows and independent researchers from 37 different countries met at the Mendes Plaza Hotel located in the city of Santos, SP - Brazil to discuss the challenges and the new frontiers of vaccinology. The SPSASV provided a critical and comprehensive view of vaccine research from basics to the current state-of-the-art techniques performed worldwide. For 10 days, we discussed all the aspects of vaccine development in 36 lectures, 53 oral presentations and 2 poster sessions. At the end of the course, participants were further encouraged to present a model of a grant proposal related to vaccine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vaccine history; WHO priorities and challenges; Immune responses; Antigen search; Delivery systems and strategies.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100201

Investigations on the role of insulin and scorpion antivenom in scorpion envenoming syndrome
Provedor de dados: 80
Autores: Radha Krishna Murthy,K.; As,Dubey; Zare Abbas,M.; Haghnazari,L.
Acute myocardiopathy in alloxan treated experimental dogs and rabbits was induced by subcutaneous (SQ) injection of scorpion venom from Mesobuthus tamulus concanesis, Pocock. Envenoming resulted in an initial transient hypertension (180-320 mm Hg.) followed by hypotension. Simultaneous administration of venom and species-specific scorpion antivenom (SAV) prevented hypertension and hypotension. Hypotension did not occur when SAV was given 60 min after envenoming. Blood glucose, triglycerides, cholesterol, amylase, insulin, glucagon, cortisol, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelet count, red blood cell (RBC) count, hemoglobin (Hb), 2,3-diphosphoglycerate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesobuthus tamulus concanesis; Pocock; Insulin therapy; Serotherapy; Alloxan; Tissue glycogen; Blood pressure; Hematological changes.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200006

Non-neurotoxic activity of Malayan krait (Bungarus candidus) venom from Thailand
Provedor de dados: 80
Autores: Charoenpitakchai,Mongkon; Wiwatwarayos,Kulachet; Jaisupa,Nattapon; Rusmili,Muhamad Rusdi Ahmad; Mangmool,Supachoke; Hodgson,Wayne C.; Ruangpratheep,Chetana; Chanhome,Lawan; Chaisakul,Janeyuth.
Abstract  Background: Envenoming by kraits (genus Bungarus) is a medically significant issue in South Asia and Southeast Asia. Malayan krait (Bungarus candidus) venom is known to contain highly potent neurotoxins. In recent years, there have been reports on the non-neurotoxic activities of krait venom that include myotoxicity and nephrotoxicity. However, research on such non-neurotoxicity activities of Malayan krait venom is extremely limited. Thus, the aim of the present study was to determine the myotoxic, cytotoxic and nephrotoxic activities of B. candidus venoms from northeastern (BC-NE) and southern (BC-S) Thailand in experimentally envenomed rats.  Methods: Rats were administered Malayan krait (BC-NE or BC-S) venom (50 μg/kg, i.m.) or 0.9% NaCl...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Krait; Bungarus candidus; Myotoxicity; Nephrotoxicity; Venom; Kidney.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100304

Assessment of Leishmania major and Leishmania braziliensis promastigote viability after photodynamic treatment with aluminum phthalocyanine tetrasulfonate (AlPcS4)
Provedor de dados: 80
Autores: JG,Pinto; CP,Soares; J,Mittmann.
Cutaneous leishmaniasis is an infectious disease caused by protozoans of the genus Leishmania, which is transmitted through the bite of hematophagous insects of the genus Lutzomyia. This study aimed at testing in vitro the phototoxic effect of aluminum phthalocyanine tetrasulfonate (AlPcS4) on the viability of Leishmania major and Leishmania braziliensis. Stationary phase promastigote forms were treated with AlPcS4 at 1.0 µM and 10.0 µM and incubated for one hour. Then 659 nm laser was applied at 5 and 10 J/cm². Parasite viability was determined by differential count using the trypan blue dye exclusion method and by monitoring growth curves for nine days. Trypan blue exclusion assay showed a significant reduction of viable parasites compared to controls,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cutaneous leishmaniasis; Antiparasitic agents; Leishmania major; Leishmania braziliensis; AlPcS4; Photodynamic therapy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300010

Scorpionism by Tityus silvestris in eastern Brazilian Amazon
Provedor de dados: 80
Autores: Coelho,Johne Souza; Ishikawa,Edna Aoba Yassui; Santos,Paulo Roberto Silva Garcez dos; Pardal,Pedro Pereira de Oliveira.
Abstract  Background  Scorpionism is a serious public health problem in Brazil. Although cases of envenomation by scorpions are frequent in Brazil, Tityus silvestris – found throughout the Amazon region – is considered of minor medical significance and with only a few descriptions in the literature. This article aims to describe for the first time the epidemiological characteristics and clinical manifestations of scorpion stings by T. silvestris that occurred in eastern Brazilian Amazon.  Methods  A prospective and observational study was carried out on 13 confirmed cases of T. silvestris envenomation registered from 2007 to 2011 in the cities of Belém and Ananindeua, Pará state, Brazil.  Results  The stings occurred mainly during daytime, at domiciliary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion sting; Scorpionism; Tityus silvestris; Envenomation; Eastern Brazilian Amazon.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100317

Inhibition of L-glutamate and GABA synaptosome uptake by crotoxin, the major neurotoxin from Crotalus durissus terrificus venom
Provedor de dados: 80
Autores: Cecchini,A.L.; Soares,A.M.; Giglio,J.R.; Amara,S.; Arantes,E.C.
This paper describes a brief study on the crotoxin mechanism of action, regarding the transport of GABA and L-glutamate in rats cortico-cerebral synaptosomes and in heterologous systems, such as COS-7 cells expressing gabaergic transporters, and C6 glioma cells and Xenopus oocytes expressing glutamatergic transporters. Crotoxin concentrations over 1 µM caused an inhibitory effect of ³H-L-glutamate and ³H-GABA, and reversibly inhibited L-glutamate uptake by C6 glioma cells. When COS-7 cells were assayed, no inhibition of the ³H-GABA transport could be evidenced. Crotoxin kept its inhibitory effect on neurotransmitters uptake even when Ca2+ ions were removed from the medium, therefore, independently of its PLA2 activity. In addition, high concentrations (2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotoxin; L-glutamate; GABA; Crotalus durissus terrificus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300006

Characteristics of hemolytic activity induced by the aqueous extract of the Mexican fire coral Millepora complanata
Provedor de dados: 80
Autores: García-Arredondo,Alejandro; Murillo-Esquivel,Luis J; Rojas,Alejandra; Sanchez-Rodriguez,Judith.
BackgroundMillepora complanata is a plate-like fire coral common throughout the Caribbean. Contact with this species usually provokes burning pain, erythema and urticariform lesions. Our previous study suggested that the aqueous extract of M. complanata contains non-protein hemolysins that are soluble in water and ethanol. In general, the local damage induced by cnidarian venoms has been associated with hemolysins. The characterization of the effects of these components is important for the understanding of the defense mechanisms of fire corals. In addition, this information could lead to better care for victims of envenomation accidents.Methods An ethanolic extract from the lyophilized aqueous extract was prepared and its hemolytic activity was compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytolysins; Hemolysins; Fire coral; Millepora complanata.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200339

Treatment of venous ulcers with surgical adhesives derived from snake venom
Provedor de dados: 80
Autores: Gatti,M. A. N.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200020

Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole
Provedor de dados: 80
Autores: Takahagi-Nakaira,E; Sugizaki,MF; Peraçoli,MTS.
In vitro tests employing microdilution to evaluate fungal susceptibility to antifungal drugs are already standardized for fermentative yeasts. However, studies on the susceptibility of dimorphic fungi such as Paracoccidioides brasiliensis employing this method are scarce. The present work introduced some modifications into antifungal susceptibility testing from the European Committee on Antimicrobial Susceptibility Testing (EUCAST), concerning broth medium and reading time, to determine minimal inhibitory concentration (MIC) of amphotericin B and itraconazole against Paracoccidioides brasiliensis. Yeast-like cells of P. brasiliensis (Pb18 strain) were tested for susceptibility to amphotericin B and itraconazole in RPMI 1640 medium, supplemented with 2%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amphotericin B; Itraconazole; P. brasiliensis; Antifungal susceptibility.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400010

Leptospira spp. and Toxoplasma gondii antibodies in vampire bats (Desmodus rotundus) in Botucatu region, SP, Brazil
Provedor de dados: 80
Autores: Zetun,CB; Hoffmann,JL; Silva,RC; Souza,LC; Langoni,H.
The destruction of natural ecosystems has caused several problems to humans and other animals; herein we investigate the close relationship among vampire bats, humans and domestic animals. Toxoplasma gondii and Leptospira spp. infections are two worldwide zoonoses that provoke serious damage to animals. To determine the prevalence of bats seropositive for toxoplasmosis and leptospirosis in the Botucatu region, 204 serum samples of vampire bats (Desmodus rotundus) were tested for T. gondii antibodies by modified agglutination test (MAT-t) and for Leptospira spp. by microscopic agglutination test (MAT-l). No animal was tested positive for T. gondii while leptospiral positivity was 7.8% for Pyrogenes, Shermani and Javanica serovars, with titers varying from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vampire bats; Zoonosis; Serological survey; Public health.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300014

Purification and partial characterization of a coagulant serine protease from the venom of the Iranian snake Agkistrodon halys
Provedor de dados: 80
Autores: Ghorbanpur,M; Zare Mirakabadi,A; Zokaee,F; Zolfagarrian,H; Rabiei,H.
Agkistrodon halys is one of several dangerous snake species in Iran. Among the most important signs and symptoms in patients envenomated by this snake is disseminated intravascular coagulation. A thrombin-like enzyme, called AH143, was isolated from Agkistrodon halys venom by gel filtration on a Sephadex G-50 column, ion-exchange chromatography on a DEAE-Sepharose and high performance liquid chromatography (HPLC) on a C18 column. In the final stage of purification, 0.82 mg of purified enzyme was obtained from 182.5 mg of venom. The purified enzyme showed a single protein band by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), under reducing conditions, and its molecular mass was found to be about 30 kDa. AH143 revealed clotting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iranian snake; Venom; Agkistrodon halys; Serine protease; Chromatography; Coagulant activity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300005

Potentiation of a novel palladium (II) complex lethality with bee venom on the human T-cell acute lymphoblastic leukemia cell line (MOLT-4)
Provedor de dados: 80
Autores: Safaeinejad,Zahra; Mohammad Nabiuni,; Nazari,Zahra.
Background : Although honeybee venom (BV) has been reported to induce apoptosis in different types of cancerous cells, its synergistic effects with customary anti-cancer drugs remain largely unknown. In the present study, we evaluated the cytotoxic effect of BV alone (as a natural product) and the synergistic cytological effects of this component in combination with [Pd (bpy) (Pi-Pydtc)]NO3 - a novel palladium complex on human T-cell lymphoblastic leukemia cells. To investigate the cytotoxic effect of the BV alone and in combination with palladium complex on MOLT-4 cells MTT assay was performed. In order to determine the apoptotic effects of BV separately and in combination with Pd (II) complex on these cells and its ability to induce apoptosis,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Bee venoms; Cytotoxicity; MOLT-4 cell line; Pd (II) complex.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100315

Evaluation of scorpion sting incidence in turkey
Provedor de dados: 80
Autores: Ozkan,O.; Uzun,R.; Adiguzel,S.; Cesaretli,Y.; Ertek,M.
Scorpion stings are common in Turkey due to its geographical location, climate and socioeconomic structure. Scorpion envenomation cases are a considerable public health problem in all regions of the country. Important health-threatening scorpions in Turkey are Androctonus crassicauda, Leiurus quinquestriatus, Mesobuthus gibbosus and M. eupeus, all of which belong to the Buthidae family. They are described to be potentially dangerous to humans. So far, there is no study about scorpion sting incidence covering all geographical regions of Turkey; therefore, in this study, we analyzed the frequency of scorpion sting cases in the country. A total of 24,261 scorpion sting cases were reported during 2005. Most of which (about 80%) occurred in the Southeastern...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion sting; Turkey; Epidemiology.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100010

Preparation and in vitro characterization of chitosan nanoparticles containing Mesobuthus eupeus scorpion venom as an antigen delivery system
Provedor de dados: 80
Autores: Mohammadpour Dounighi,N; Eskandari,R; Avadi,MR; Zolfagharian,H; Mir Mohammad Sadeghi,A; Rezayat,M.
Hydrophilic nanoparticles have been widely investigated in recent years as delivery systems for therapeutic macromolecules such as antigens. In the present study Mesobuthus eupeus venom-loaded chitosan nanoparticles were prepared via ionic gelation of tripolyphosphate (TPP) and chitosan. The optimum encapsulation efficiency (91.1%) and loading capacity (76.3%) were obtained by a chitosan concentration of 2 mg/mL, chitosan-to-TPP mass ratio of 2 and M. eupeus venom concentration of 500 µg/mL. The average nanoparticle size at optimum conditions was determined by Zetasizer (Malvern Instruments, UK). The nanoparticle size was about 370 nm (polydispersity index: 0.429) while the zeta potential was positive. Transmission electron microscope (TEM) imaging showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanoparticle; Chitosan; Tripolyphosphate; Venom; Antigen delivery system; Mesobuthus eupeus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100006

Candida spp.: manual identification (reference method) and automated identification (Vitek system platform)
Provedor de dados: 80
Autores: Mondelli,AL; Niéro-Melo,L; Bagagli,E; Camargo,CH; Bruder-Nascimento,A; Sugizaki,MF; Carneiro,MV; Villas Boas,PJF.
Yeasts are becoming a common cause of nosocomial fungal infections that affect immunocompromised patients. Such infections can evolve into sepsis, whose mortality rate is high. This study aimed to evaluate the viability of Candida species identification by the automated system Vitek-Biomerieux (Durham, USA). Ninety-eight medical charts referencing the Candida spp. samples available for the study were retrospectively analyzed. The system Vitek-Biomerieux with Candida identification card is recommended for laboratory routine use and presents 80.6% agreement with the reference method. By separate analysis of species, 13.5% of C. parapsilosis samples differed from the reference method, while the Vitek system wrongly identified them as C. tropicalis, C....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Candidemia; Candida spp.; Identification; Automated identification.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300011

Antiviral activity of animal venom peptides and related compounds
Provedor de dados: 80
Autores: Mata,Élida Cleyse Gomes da; Mourão,Caroline Barbosa Farias; Rangel,Marisa; Schwartz,Elisabeth Ferroni.
Abstract Viruses exhibit rapid mutational capacity to trick and infect host cells, sometimes assisted through virus-coded peptides that counteract host cellular immune defense. Although a large number of compounds have been identified as inhibiting various viral infections and disease progression, it is urgent to achieve the discovery of more effective agents. Furthermore, proportionally to the great variety of diseases caused by viruses, very few viral vaccines are available, and not all are efficient. Thus, new antiviral substances obtained from natural products have been prospected, including those derived from venomous animals. Venoms are complex mixtures of hundreds of molecules, mostly peptides, that present a large array of biological activities and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antiretroviral agents; Antiviral agents; HIV; Scorpion venom; Snake venom; Amphibian venom; Insect venom; Marine animal peptides.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100200

Detection and identification of huwentoxin-IV interacting proteins by biotin-avidin chemistry combined with mass spectrometry
Provedor de dados: 80
Autores: Yu,Hai; Liu,Hui; Yan,Yizhong; Duan,Zhigui; Wang,Xianchun.
Background : Numerous spider toxins are of interest as tools for neurophysiological research or as lead molecules for the development of pharmaceuticals and insecticides. Direct detection and identification of the interacting proteins of a spider toxin are helpful for its action-mechanism analysis and practical application. The present study employed a combinative strategy for the analysis of interacting proteins of huwentoxin-IV (HWTX-IV), a peptidic neurotoxin from the venom of the spiderSelenocosmia huwena.Results : HWTX-IV was first lightly labeled with biotin under the optimized mild experimental conditions and the toxin labeled with a single biotin group (monobiotinylated HWTX-IV) was demonstrated by electrophysiological experiments to retain its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotin labeling; Huwentoxin-IV; Far-western blotting; Interacting protein; Mass spectrometry.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200328

Epidemiological, clinical, and evolution characteristics of tuberculosis in the Bauru region, São Paulo
Provedor de dados: 80
Autores: Monti,J. F. C.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100015

Crotalus durissus terrificus crotapotin naturally displays preferred positions for amino acid substitutions
Provedor de dados: 80
Autores: Oliveira,Laudicéia Alves de; Ferreira Jr,Rui Seabra; Barraviera,Benedito; Carvalho,Francilene Capel Tavares de; Barros,Luciana Curtolo de; Santos,Lucilene Delazari dos; Pimenta,Daniel Carvalho.
Abstract  Background  Classically, Crotalus durissus terrificus (Cdt) venom can be described, according to chromatographic criteria, as a simple venom, composed of four major toxins, namely: gyroxin, crotamine, crotoxin and convulxin. Crotoxin is a non-covalent heterodimeric neurotoxin constituted of two subunits: an active phospholipase A2 and a chaperone protein, termed crotapotin. This molecule is composed of three peptide chains connected by seven disulfide bridges. Naturally occurring variants/isoforms of either crotoxin or crotapotin itself have already been reported.  Methods  The crude Cdt venom was separated by using RP-HPLC and the toxins were identified by mass spectrometry (MS). Crotapotin was purified, reduced and alkylated in order to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durissus terrificus; Venom; Crotoxin; Crotapotin; Isoforms.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100319

Bioecology and chemical diversity of abdominal glands in the iranian samsum ant Pachycondyla sennaarensis (Formicidae: Ponerinae)
Provedor de dados: 80
Autores: Nikbakhtzadeh,MR; Akbarzadeh,K; Tirgari,S.
The genus Pachycondyla is a large group of ants in the Ponerini tribe, known mostly from tropical and subtropical regions. Pachycondyla sennaarensis, the so-called Samsum ant in the Middle East, is distributed throughout the African tropics, Arabian Peninsula and Iran, where it is responsible for many cases of insect-induced dermal lesions and systemic reactions in humans. Populations of P. sennaarensis were studied in two regions of Iran and some aspects of their biology, ecology and medical importance are herein presented. Colonies of P. sennaarensis contain less than 850 workers that live in complicated underground galleries approximately one meter deep. Because of the harsh weather conditions of southern Iran, they can survive only in human disturbed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Samsum ant; Sting; Ants; Formicidae; Ponerinae; Pachycondyla.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300012

Antivenoms: potency or median effective dose, which to use?
Provedor de dados: 80
Autores: Morais,V; Ifran,S; Berasain,P; Massaldi,H.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200002

Effects of Garlic (Alliumsativum) and chloramphenicol on growth performance, physiological parameters and survival of Nile tilapia (Oreochromis niloticus)
Provedor de dados: 80
Autores: Shalaby,A. M.; Khattab,Y. A.; Abdel Rahman,A. M.
We studied and compared the effects of chloramphenicol antibiotic and garlic (Allium sativum), used as immunostimulants and growth promoters, on some physiological parameters, growth performance, survival rate, and bacteriological characteristics of Nile tilapia (Oreochromis niloticus). Fish (7±1g/fish) were assigned to eight treatments, with three replicates each. Treatment groups had a different level of Allium sativum (10, 20, 30, and 40g/kg diet) and chloramphenicol (15, 30, and 45mg/kg diet) added to their diets; the control group diet was free from garlic and antibiotic. Diets also contained 32% crude protein (CP) and were administered at a rate of 3% live body weight twice daily for 90 days. Results showed that the final weight and specific growth...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium sativum; Chloramphenicol; Nile tilapia; Growth parameter; Hematology; Biochemistry; Aeromonas hydrophila.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200003

Exploring the in vivo wound healing effects of a recombinant hemolin from the caterpillar Lonomia obliqua
Provedor de dados: 80
Autores: Sato,Ana Claudia; Bosch,Rosemary Viola; Will,Sonia Elisabete Alves; Alvarez-Flores,Miryam Paola; Goldfeder,Mauricio Barbugiani; Pasqualoto,Kerly Fernanda Mesquita; Silva,Bárbara Athayde Vaz Galvão da; Andrade,Sonia Aparecida de; Chudzinski-Tavassi,Ana Marisa.
Abstract  Background  Hemolin proteins are cell adhesion molecules from lepidopterans involved in a wide range of cell interactions concerning their adhesion properties. However, hemolin’s roles in cell proliferation and wound healing are not fully elucidated. It has been recently reported that rLosac, a recombinant hemolin from the caterpillar Lonomia obliqua, presents antiapoptotic activity and is capable of improving in vitro wound healing. Therefore, this study aimed to explore rLosac’s in vivo effects using a skin wound healing model in rats.  Methods  Circular full-thickness wounds in the rat dorsum skin were treated either with rLosac, or with saline (control), allowing healing by keeping the wounds occluded and moist. During the wound healing, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemolin; RLosac; Wound healing; Collagen.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100324

Comparison of proteins, lethality and immunogenic compounds of Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) venom obtained by different methods
Provedor de dados: 80
Autores: Ozkan,O.; Kar,S.; Güven,E.; Ergun,G.
Scorpions are venomous arthropods of the class Arachnida and are considered relatives of spiders, ticks and mites. There are approximately 1,500 species of scorpions worldwide, which are characterized by an elongated body and a segmented tail that ends in a venomous stinger. No specific treatment is available for scorpion envenomation, except for the use of antivenom. The current study aimed at comparing protein content and lethality of Androctonus crassicauda venom extracted by two different methods (electric stimulation and maceration of telsons). The LD50 calculated by probit analysis was 1.1mg/kg for venom obtained by electric stimulation and 39.19mg/kg for venom obtained by maceration of telsons. In the electrophoretic analysis, protein bands of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venoms; Androctonus crassicauda; Gland maceration; Electric stimulation; Immunogenicity; Lethality.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400013

DNA damage in BALB/c mice infected with Lacazia loboi and its relation to nutritional status
Provedor de dados: 80
Autores: Barbosa,Adriana Sierra Assencio Almeida; Oliveira,Larissa Ragozo Cardoso de; Tavares,Francilene Capel; Lima,Carlos Roberto Gonçalves de; Diório,Suzana Madeira; Calvi,Sueli Aparecida; Vilani-Moreno,Fátima Regina; Pereira,Paulo Câmara Marques.
AbstractBackground Jorge Lobo’s disease, also known as lacaziosis, is a cutaneous-subcutaneous mycosis with chronic evolution. It is caused by the fungus Lacazia loboi. Herein we report a study that relates the genotoxicity caused by L. loboi in isogenic mice with nutritional status, through a normal or restricted diet.Methods DNA damage was assessed in the peripheral blood by the comet assay (tail intensity).Results The results for leukocytes showed increases in the mean tail intensity in mice under dietary restriction, in infected mice under dietary restriction and in infected mice ingesting a normal diet.Conclusion These results indicate that dietary restriction and L. loboi infection may increase DNA damage levels in mice, as detected by the comet assay.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Jorge Lobo’ disease; Lacazia loboi; Malnutrition; Mice; Genotoxicity; Comet assay.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100317

Characterization of an antibacterial peptide from indian cobra (Naja naja) venom
Provedor de dados: 80
Autores: Sachidananda,M. K.; Murari,S. K.; Channe Gowda,D.
Due to the development of antibiotic resistance in microorganisms, antimicrobial peptides from natural sources have attracted attention in recent times. Several antimicrobial peptides have been isolated from a wide range of animal sources, particularly snake venoms. Naja naja venom showed antibacterial as well as direct and indirect hemolytic activities, and an antibacterial peptide was purified through gel permeation and ion exchange chromatography. Its molecular mass was 2491Da, which was determined using Matrix Assisted Laser Desorption/Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry and the amino acids sequence of the N-terminus was DEQSTHGAYVWKL. The purified peptide showed potent antibacterial activity against Gram-negative and Gram-positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Indian cobra; Naja naja; Snake venom antibacterial peptide; Hemolytic activity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200004

Universities and neglected diseases: it is not enough to have the knowledge, it must be applied
Provedor de dados: 80
Autores: Caramori,CA; Barraviera,B.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100001

Some arachnidan peptides with potential medical application
Provedor de dados: 80
Autores: De Lima,ME; Borges,MH; Verano-Braga,T; Torres,FS; Montandon,GG; Cardoso,FL; Peixoto,KDBA; Cardoso-Jr,HC; Benelli,MT.
The search for new active drugs that can alleviate or cure different diseases is a constant challenge to researchers in the biological area and to the pharmaceutical industry. Historically, research has focused on the study of substances from plants. More recently, however, animal venoms have been attracting attention and studies have been successful in addressing treatment of accidents. Furthermore, venoms and their toxins have been considered good tools for prospecting for new active drugs or models for new therapeutic drugs. In this review, we discuss some possibilities of using different toxins, especially those from arachnid venoms, which have shown some potential application in diseases involving pain, hypertension, epilepsy and erectile dysfunction....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arachnidan toxins; Pain; Epilepsy; Cardiovascular effect; Priapism; Erectile function; Scorpion; Spider.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100004

Etiology and drug susceptibility of chronic supurative otitis media microorganisms in patients with cleft palate or cleft lip palate
Provedor de dados: 80
Autores: Weckwerth,P. H.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100019

Butantan Institute: strategies to obtain scorpions for the production of anti-scorpion serum
Provedor de dados: 80
Autores: Lucas,SM; Goldoni,PAM; Candido,DM; Knysak,I.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400002

Preparation and neutralization efficacy of IgY antibodies raised against Deinagkistrodon acutus venom
Provedor de dados: 80
Autores: Liu,Jinhua; He,Qiyi; Wang,Wenwen; Zhou,Bin; Li,Bo; Zhang,Yingfeng; Luo,Cong; Chen,Diancheng; Tang,Jia; Yu,Xiaodong.
Abstract  Background  The five-paced pit viper (Deinagkistrodon acutus), endemic to China and northern Vietnam, is responsible for most snakebites in the Chinese territory. Antivenom produced from horses is the main treatment for snakebites, but it may cause numerous clinical side effects and have other disadvantages involved in their production such as the welfare of animals. The present study was conducted aiming to develop an alternative antibody (IgY) from the egg yolk of leghorn chickens immunized with snake venom.  Methods  IgY from the egg yolk of white leghorn chickens previously immunized intramuscularly with D. acutus venom was extracted by water, precipitated by ammonium sulfate and purified by affinity chromatographic system. IgY was identified...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IgY antibody; Egg yolk; Snake venom; Snakebite; Deinagkistrodon acutus; Venom neutralization.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100310

Challenges and perspectives of Chagas disease: a review
Provedor de dados: 80
Autores: Pereira,Paulo Câmara Marques; Navarro,Elaine Cristina.
Chagas disease (CD), also known as American trypanosomiasis, is caused by the flagellated protozoan Trypanosoma cruzi, and affects an estimated 8 to 10 million people worldwide. In Latin America, 25 million people live in risk areas, while in 2008 alone, 10,000 CD-related deaths were reported. This review aimed to evaluate the challenges of CD control, future perspectives, and actions performed worldwide to control expansion of the disease and its impact on public health in Latin America.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Control of Chagas disease; Epidemiology; Transmission.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100206

Purification and biological effects of a C-type lectin isolated from Bothrops moojeni
Provedor de dados: 80
Autores: Barbosa,PSF; Martins,AMC; Toyama,MH; Joazeiro,PP; Beriam,LOS; Fonteles,MC; Monteiro,HSA.
Snake venom proteins from the C-type lectin family have very distinct biological activities despite their highly conserved primary structure, which is homologous to the carbohydrate recognition region of true C-type lectins. We purified a lectin-like protein (BmLec) from Bothrops moojeni venom and investigated its effect on platelet aggregation, insulin secretion, antibacterial activity, and isolated kidney cells. The BmLec was purified using two chromatographic steps: affinity chromatography and reverse phase high performance liquid chromatography (HPLC). BmLec showed a dose-dependent platelet aggregation and significantly decreased the bacterial growth rate in approximately 15%. During scanning electron microscopy, the profile of Xanthomonas axonopodis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops moojeni; Kidney; Platelet aggregation; Insulin; Antibacterial activity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300016

Use of skin graft "punch graft" type for the healing of leg ulcers in treated hansen's disease patients
Provedor de dados: 80
Autores: Cardia,Carla Christiane de Oliveira.
Tipo: Info:eu-repo/semantics/other
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200014

Are international partnerships important? Report of an experience
Provedor de dados: 80
Autores: de Souza,LR.
Tipo: Info:eu-repo/semantics/other
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400001

Relationship among Apis mellifera L. stings, swarming and climate conditions in the city of Rio Claro, SP, Brazil
Provedor de dados: 80
Autores: Pereira,AM; Chaud-Netto,J; Bueno,OC; Arruda,VM.
The presence of bees (Apis mellifera L.) in urban areas has increased in recent years due to environmental disturbances caused by humans. Bee migration to cities may provoke serious accidents, since some people present allergic reactions to their venoms. In Rio Claro city, São Paulo state, Brazil, the number of calls to the fire brigade for removal of bee swarms, and the number admissions in local hospitals due to bee stings were investigated during 2002 and 2003, and a correlation between these data and the average temperature, rainfall and relative humidity was found. The study period was divided into three phases according to the number of times that the fire brigade was called to remove swarms (263 times): January to July 2002 - 51 calls (19.39%);...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Synanthropism; Reproductive swarms; Absconding; Environmental variables; Honeybees.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400016

Frequency of rhabdiasid nematodes in wild Crotalus durissus terrificus (serpentes, viperidae) from Botucatu region, São Paulo state, Brazil
Provedor de dados: 80
Autores: Silva,R. J.; Nogueira,M. F.; Barrella,T. H.; Takahira,R. K.
The aim of the present study was to evaluate the frequency of rhabdiasid nematodes in recently captured Crotalus durissus terrificus snakes from São Paulo State, Brazil. Fifty snakes (34 males and 16 females) were studied and each one was evaluated for the presence of that nematode at the moment of receipt at the Institution and after 90 days of quarantine inside individual cages. Tracheopulmonary washeswere examined. Snakes that died during quarantine underwent necropsy and lung examination. Analysis of the results obtained at the two evaluation times (0 and 90 days), in addition to the data obtained during necropsies, showed that 44% (18 males and 4 females) of the C. d. terrificus snakes were naturally infected by rhabdiasid nematodes. These data...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rhabdias; Nematoda; Frequency; Crotalus durissus terrificus; Viperidae; Snakes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100009

American cutaneous leishmaniasis in dogs from an endemic urban area in Cianorte municipality, Paraná State, Brazil
Provedor de dados: 80
Autores: Cerino,DA; Veloso,J; Perles,TF; Zanzarini,PD; Lonardoni,MVC; Silveira,TGV.
American cutaneous leishmaniasis (ACL) was investigated in dogs from an urban endemic area in Cianorte, Paraná state, Brazil. Of 169 studied dogs, none presented suspected ACL lesions. Eleven animals (6.6%) had anti-Leishmania braziliensis antibodies (titers &gt; 40) detected by the immunofluorescent antibody test (IFAT) while four (2.4%) showed L. braziliensis-complex DNA by the polymerase chain reaction (PCR). Although no associations were found between IFAT or PCR results and age, sex, origin, free-roaming animals or length of residence at the address, the majority of IFAT- or PCR-positive dogs were from the urban area of the city and were allowed to roam freely beyond their neighborhood. The presence of anti-Leishmania braziliensis antibodies and L....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania braziliensis; Fluorescent antibody technique; Polymerase chain reaction; Urban park.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200007

Microbiological analysis of water used in hydrotherapy
Provedor de dados: 80
Autores: Perestrelo,M. F.; Norberg,A. N.; Guerra-Sanches,F.; Torres,A. C.; Pile,E.
Water used in hydrotherapy units of Nova Iguaçu and Nilópolis, Rio de Janeiro State, Brazil, was microbiologically analyzed. Thirty samples (5ml each) were weekly collected from September 2001 to June 2002 before the beginning and after the end of activities in the units. For analysis, routine techniques were used, which showed the presence of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella spp, Candida spp, Penicillium spp, Epidermophyton spp, Aspergillus fumigatus, Aspergillus niger, Aspergillus spp, Cephalosporium spp, Cladosporium spp, Trichophyton rubrum, and Trichophyton spp. Results indicated a need for improving hygienic conditions, suggesting that water might be a contamination source in the evaluated units.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrotherapy; Water; Microorganisms; Bacteria; Fungi.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300006

Feeding history of Crotalus durissus terrificus snakes by the analysis of carbon-13 (δ13C) isotope from the rattle
Provedor de dados: 80
Autores: Martinez,M. G.
Tipo: Info:eu-repo/semantics/article
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100019

Evaluation of canine and feline leishmaniasis by the association of blood culture, immunofluorescent antibody test and polymerase chain reaction
Provedor de dados: 80
Autores: Braga,Audrey Rennó Campos; Langoni,Hélio; Lucheis,Simone Baldini.
Background: This study aimed to evaluate the occurrence of Leishmaniaspp. in dogs and cats from Botucatu, São Paulo state, and Campo Grande, Mato Grosso do Sul state, Brazil, by the association of three diagnostic tests: blood culture in liver infusion tryptose medium, immunofluorescent antibody test and polymerase chain reaction. Fifty blood samples of dogs and cats from the Center for Zoonosis Control in Campo Grande, an area endemic for canine visceral leishmaniasis, were collected randomly, as well as canine and feline blood samples from the Municipal Kennel and Animal Protection Association in Botucatu, currently considered a transmission-free, non-endemic area.Results: Of the 50 dog blood cultures from Botucatu, three (6%) were positive and of the 50...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Cat; Diagnosis; Leishmaniasis; Zoonosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200323

Next-generation sequencing analysis reveals high bacterial diversity in wild venomous and non-venomous snakes from India
Provedor de dados: 80
Autores: Krishnankutty,Sajesh Puthenpurackal; Muraleedharan,Megha; Perumal,Rajadurai Chinnasamy; Michael,Saju; Benny,Jubina; Balan,Bipin; Kumar,Pramod; Manazhi,Jishnu; Kumar,Bangaruswamy Dhinoth; Santhosh,Sam; Thomas,George; Gupta,Ravi; Zachariah,Arun.
Abstract  Background: The oral cavities of snakes are replete with various types of bacterial flora. Culture-dependent studies suggest that some of the bacterial species are responsible for secondary bacterial infection associated with snakebite. A complete profile of the ophidian oral bacterial community has been unreported until now. Therefore, in the present study, we determined the complete bacterial compositions in the oral cavity of some snakes from India.  Methods: Total DNA was isolated from oral swabs collected from three wild snake species (Indian Cobra, King Cobra and Indian Python). Next, the DNA was subjected to PCR amplification of microbial 16S rRNA gene using V3-region-specific primers. The amplicons were used for preparation of DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial community; Next-generation sequencing; Venomous snake; Hypervariable region.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100331

Clinical aspects of envenomation caused byTityus obscurus (Gervais, 1843) in two distinct regions of Pará state, Brazilian Amazon basin: a prospective case series
Provedor de dados: 80
Autores: Pardal,Pedro PO; Ishikawa,Edna AY; Vieira,José LF; Coelho,Johne S; Dórea,Regina CC; Abati,Paulo AM; Quiroga,Mariana MM; Chalkidis,Hipócrates M.
Background:Scorpion envenomations are a major public health problem in Brazil, whose most dangerous cases are attributable to the genus Tityus.This study was designed to compare the clinical and demographic features of envenomations by 77tyus obscurus in two areas of the state of Pará located in the Amazon basin. Were compared demographic findings, local and systemic signs and symptoms of human envenomations caused byT. obscurus that occurred in western and eastern areas of the state.Results:Forty-eight patients with confirmed envenomation by T. obscurus were evaluated from January 2008 to July 2011. Most of them came from the eastern region, where male and female patients were present in similar numbers, while males predominated in the west. Median age...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpionism; Tityus obscurus; Envenoming; Neurological symptoms; Brazilian Amazon.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200321

Cytokines profile and clinical-metabolic alterations in HIV-1-infected individuals with and without lypodistrophy
Provedor de dados: 80
Autores: Cardoso,L. C. R. Pontes.
Tipo: Info:eu-repo/semantics/other
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100016

Metabolism of pectenotoxins in brown crabs Cancer pagurus fed blue mussels Mytilus edulis
Provedor de dados: 80
Autores: Zhaoxin,Li.
Tipo: Info:eu-repo/semantics/article
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300018

Interactions between environment, wild animals and human leptospirosis
Provedor de dados: 80
Autores: Ullmann,LS; Langoni,H.
Leptospirosis, a worldwide distributed zoononis caused by bacteria of the genus Leptospira (antigenically classified into serovars), may be direct or indirectly transmitted through infected urine or environment. Several domestic and wild animals are leptospirosis reservoirs. The disease presents occupational character since it is widely reported in professionals that work in humid environments - such as sewage workers and fishermen - and in places where rodents or susceptible animals are found, like slaughterhouses and veterinary clinics. In developing countries, outbreaks are related to lack of sanitation, overcrowding in inadequate housing and climatic conditions. In developed countries, sporadic cases occur in aquatic recreational activities including...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira spp.; Leptospirosis; Public health; Occupational disease; Recreational disease.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200002

Molecular typing of bacterial strains isolated from the clinics of surgery and face traumatology at Ribeirão Preto University
Provedor de dados: 80
Autores: Rodrigues,M. V. Pimenta.
Tipo: Info:eu-repo/semantics/other
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400018

Cytolytic and systemic toxic effects induced by the aqueous extract of the fire coral Millepora alcicornis collected in the Mexican Caribbean and detection of two types of cytolisins
Provedor de dados: 80
Autores: Hernández-Matehuala,Rosalina; Rojas-Molina,Alejandra; Vuelvas-Solórzano,Alma Angelica; Garcia-Arredondo,Alejandro; Ibarra Alvarado,Cesar; Olguín-López,Norma; Aguilar,Manuel.
AbstractBackground Millepora alcicornis is a branching hydrocoral common throughout the Caribbean Sea. Like other members of this genus, this species is capable of inducing skin eruptions and blisters with severe pain after contact. In the present study, we investigated the toxicity of theM. alcicornis aqueous extract on several animal models. Considering that some cnidarian hemolysins have been associated to local tissue damage, since they also induce lysis of other cell types, we also made a partial characterization of the hemolytic activity of M. alcicornis aqueous extract. This information is important for understanding the defense mechanisms of the “fire corals”.Methods The effects of pH, temperature, and some divalent cations on the hemolytic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fire coral; Millepora alcicornis; Hemolysis; Systemic toxicity; Electronic microscopy.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100342

Proteomics and antivenom immunoprofiling of Russell’s viper (Daboia siamensis) venoms from Thailand and Indonesia
Provedor de dados: 80
Autores: Lingam,Thava Malar Changra; Tan,Kae Yi; Tan,Choo Hock.
Background The Eastern Russell’s viper, Daboia siamensis, is a WHO Category 1 medically important venomous snake. It has a wide but disjunct distribution in Southeast Asia. The specific antivenom, D. siamensis Monovalent Antivenom (DsMAV-Thailand) is produced in Thailand but not available in Indonesia, where a heterologous trivalent antivenom, Serum Anti Bisa Ular (SABU), is used instead. This study aimed to investigate the geographical venom variation of D. siamensis from Thailand (Ds-Thailand) and Indonesia (Ds-Indonesia), and the immunorecognition of the venom proteins by antivenoms.  Methods:  The venom proteins were decomplexed with reverse-phase high-performance liquid chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venomics; Antivenomics; Eastern Russell's viper; Neutralization.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100302

Heterologous expression of an insecticidal gene from the armed spider (Phoneutria nigriventer)
Provedor de dados: 80
Autores: Figueiredo,J. E. F.; Kalapothakis,E.; Gomez,M. V.; Bressan,W.
Insect-pests are global problems that cause severe damage to crop plants, and their control is commonly based on chemical insecticides. However, negative effects of pesticides on the environment and human health emphasize the necessity to develop alternative methods for insect-pest control. In the present study, a gene coding for the insecticidal peptide TX4(6-1) of the Brazilian armed spider (Phoneutria nigriventer) was cloned in fusion with maltose binding protein (MBP) and expressed in Escherichia coli. The affinity purified protein MBP-GlyTX4 was cleaved with the Xa factor and used for a bioassay against Spodoptera frugiperda and rabbit immunization. Five micrograms GlyTX4 protein injected into the hemocoel of larvae and abdominal cavity of adults...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinsecticide; Molecular cloning; Heterologous expression; Escherichia coli; Spodoptera frugiperda.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200006

Epidemiology of envenomations by terrestrial venomous animals in Brazil based on case reporting: from obvious facts to contingencies
Provedor de dados: 80
Autores: Chippaux,Jean-Philippe.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Envenomation; Snakes; Scorpions; Spiders; Caterpillars; Africanized bees; Brazil; Epidemiology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100319

Purification of an L-amino acid oxidase from Bungarus caeruleus (Indian krait) venom
Provedor de dados: 80
Autores: More,SS; Kiran,KM; Veena,SM; Gadag,JR.
Snake venoms are rich in enzymes such as phospholipase A2, proteolytic enzymes, hyaluronidases and phosphodiesterases, which are well characterized. However, L-amino acid oxidase (LAO EC.1.4.3.2) from snake venoms has not been extensively studied. A novel L-amino acid oxidase from Bungarus caeruleus venom was purified to homogeneity using a combination of ion-exchange by DEAE-cellulose chromatography and gel filtration on Sephadex® G-100 column. The purified monomer of LAO showed a molecular mass of 55 ±1 kDa estimated by SDS-PAGE. The specific activity of purified LAO was 6,230 ± 178 U/min/mg, versus 230 ± 3.0 U/min/mg for the whole desiccated venom, suggesting a 27-fold purification with a 25% yield. Optimal pH and temperature for maximum purified enzyme...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-amino acid oxidase; Bungarus caeruleus; Platelet aggregation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100007

Trichomoniasis in Bothrops jararaca (serpentes, viperidae)
Provedor de dados: 80
Autores: Vilela,F. C.; Silva,M. G. da; Barrella,T. H.; Silva,R. J. da.
We describe a case of trichomoniasis in a Bothrops jararaca (Serpentes, Viperidae) donated to the Center for the Study of Venoms and Venomous Animals - CEVAP/UNESP. The animal had diarrhea with great quantity of flagellated protozoa in the feces. Microscopic examination of fecal smears stained with Giemsa revealed the presence of trichomonads, morphologically similar to Trichomonas acosta. Trichomonads were not detected in fecal exams after treatment with a single dose of 40 mg/kg metronidazole (Flagyl®).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichomoniasis; Trichomonas sp.; Bothrops jararaca; Treatment; Metronidazole.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100007

Arachnids of medical importance in Brazil: main active compounds present in scorpion and spider venoms and tick saliva
Provedor de dados: 80
Autores: Cordeiro,Francielle A.; Amorim,Fernanda G.; Anjolette,Fernando A. P.; Arantes,Eliane C.
Arachnida is the largest class among the arthropods, constituting over 60,000 described species (spiders, mites, ticks, scorpions, palpigrades, pseudoscorpions, solpugids and harvestmen). Many accidents are caused by arachnids, especially spiders and scorpions, while some diseases can be transmitted by mites and ticks. These animals are widely dispersed in urban centers due to the large availability of shelter and food, increasing the incidence of accidents. Several protein and non-protein compounds present in the venom and saliva of these animals are responsible for symptoms observed in envenoming, exhibiting neurotoxic, dermonecrotic and hemorrhagic activities. The phylogenomic analysis from the complementary DNA of single-copy nuclear protein-coding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arachnid toxins; Scorpion venom; Spider venom; Tick saliva.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100211

Comparison between two methods of scorpion venom milking in Morocco
Provedor de dados: 80
Autores: Oukkache,Naoual; Chgoury,Fatima; Lalaoui,Mekki; Cano,Alejandro Alagón; Ghalim,Noreddine.
Background The present study compared two methods used successfully in a large-scale program for the collection of scorpion venoms, namely the milking of adult scorpions via manual and electrical stimulation. Results Our immunobiochemical characterizations clearly demonstrate that regularly applied electrical stimulation obtains scorpion venom more easily and, most importantly, in greater quantity. Qualitatively, the electrically collected venom showed lack of hemolymph contaminants such as hemocyanin. In contrast, manual obtainment of venom subjects scorpions to maximal trauma, leading to hemocyanin secretion. Our study highlighted the importance of reducing scorpion trauma during venom milking. Conclusions In conclusion, to produce high quality antivenom...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venoms; Manual stimulation; Electrical stimulation; Antivenom; Lethality.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100303

Parthenogenesis in scorpions: some history - new data
Provedor de dados: 80
Autores: Lourenço,W. R.
Parthenogenesis, a rare phenomenon in Chelicerates, apart from mites, is reviewed in scorpions, notably Tityus serrulatus Lutz &amp;amp; Mello from Brazil, Tityus columbianus (Thorell) from Colombia and Tityus metuendus Pocock from Peru and Brazil. Thelytokous parthenogenesis (with all-female broods) is most often observed. The only known exception to this is T. metuendus in which arrhenotoky (all-male broods) has been observed. In the present paper, current ideas regarding parthenogenesis in scorpions are summarized. The notion of geographic parthenogenesis ('parthénogenèse géographique'), coined by Vandel in 1928, is discussed. This rule is tentatively exemplified by a new case of parthenogenesis reported in a scorpion of the genus Tityus C. L. Koch,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpions; Geographic parthenogenesis; Review; Thelytoky; Arrhenotoky; Deutherotoky.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100003

Change the QUALIS criteria!
Provedor de dados: 80
Autores: Andriolo,A; Barbosa,AJA; Hernandez,AJ; Camargos,AF; Barraviera,B; Kadunc,BV.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400001

Report of 15 injuries caused by lionfish (pterois volitans) in aquarists in Brazil: a critical assessment of the severity of envenomations
Provedor de dados: 80
Autores: Haddad Jr,Vidal; Stolf,Hamilton Ometto; Risk,José Yamin; França,Francisco OS; Cardoso,João Luiz Costa.
AbstractLionfish are venomous fish that belong to the Scorpaenidae family. Individuals of this family and those of the Synanceiidae family comprise most of the existing venomous fish in the world. Lionfish are originally found in the Indo-Pacific, but they have received special attention in the last years for their dissemination in the Atlantic Ocean, with the emergence of large populations in the USA, Caribbean and South America. Because of its beauty, this fish has always been present in private and commercial aquariums around the world. Herein, we describe 15 envenomations in aquarists registered in a period of eighteen years (1997–2014). The stings caused excruciating pain and marked inflammation, with local erythema, edema, heat, paleness and...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Pterois volitans; Pterois miles; Venomous fish; Animals invasions; Aquarism; Lionfish; Envenomations.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100411

Evaluation of macrophages and T cells activity in murine experimental models of high and low antibody-producers (selection IV-A) infected with Paracoccidioides brasiliensis
Provedor de dados: 80
Autores: Pedigone,C. C.
Tipo: Info:eu-repo/semantics/article
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400026

American visceral leishmaniasis: disease control strategies in dracen microregion in alta paulista, SP, Brazil
Provedor de dados: 80
Autores: D'Andrea,LAZ; Camargo-Neves,VLF; Sampaio,SMP; Kronka,SN; Sartor,IF.
Despite measures adopted to control American visceral leishmaniasis (AVL), the disease is spreading in a fast and worrying way throughout western São Paulo state. The aim of this work was to study the variables involved in the disease cycle as well as the effectiveness of controlling measures. The study was carried out in the microregion of Dracena, which is composed of twelve cities and belongs to Alta Paulista, a region of western São Paulo. The necessary data were provided by the Superintendence for Endemic Disease Control and Adolfo Lutz Institute, Regional Laboratory of Presidente Prudente. From August 2005 to January 2008, the following factors were observed: detection of phlebotomine sandflies in the cities and periods in which dogs or humans were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Seroprevalence; Control strategies; São Paulo; Brazil.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200012

Pharmacological and partial biochemical characterization of Bmaj-9 isolated from Bothrops marajoensis snake venom
Provedor de dados: 80
Autores: Galbiatti,C; Rocha,T; Randazzo-Moura,P; Ponce-Soto,LA; Marangoni,S; Cruz-Höfling,MA; Rodrigues-Simioni,L.
Bmaj-9, a basic PLA2 (13679.33 Da), was isolated from Bothrops marajoensis snake venom through only one chromatographic step in reversed phase HPLC on ¼-Bondapak C-18 column. The amino acid composition showed that Bmaj-9 had a high content of Lys, His, and Arg, typical of a basic PLA2. The sequence of Bmaj-9 contains 124 amino acid residues with a pI value of 8.55, such as DLWQWGQMIL KETGKLPFSY YTAYGCYCGW GGRGGKPKAD TDRCCFVHDC, revealing a high homology with Asp49 PLA2 from other snake venoms. It also exhibited a pronounced phospholipase A2 activity when compared with crude venom. In chick biventer cervicis preparations, the time for 50% and 100% neuromuscular paralysis was respectively (in minutes): 110 ± 10 (1 µg/mL); 40 ± 6 and 90 ± 2 (5 µg/mL); 30 ± 3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops marajoensis; Snake venom; Asp49 PLA2; Neuromuscular blockade.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100008

Effects of Brazilian scorpion venoms on the central nervous system
Provedor de dados: 80
Autores: Nencioni,Ana Leonor Abrahão; Beraldo Neto,Emidio; Freitas,Lucas Alves de; Dorce,Valquiria Abrão Coronado.
Abstract In Brazil, the scorpion species responsible for most severe incidents belong to the Tityus genus and, among this group, T. serrulatus, T. bahiensis, T. stigmurus and T. obscurus are the most dangerous ones. Other species such as T. metuendus, T. silvestres, T. brazilae, T. confluens, T. costatus, T. fasciolatus and T. neglectus are also found in the country, but the incidence and severity of accidents caused by them are lower. The main effects caused by scorpion venoms - such as myocardial damage, cardiac arrhythmias, pulmonary edema and shock - are mainly due to the release of mediators from the autonomic nervous system. On the other hand, some evidence show the participation of the central nervous system and inflammatory response in the process....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian scorpions; Central nervous system; Scorpion venom; Scorpion toxins.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100202

Epidemiological investigation on envenomation: from theory to practice
Provedor de dados: 80
Autores: Chippaux,JP.
A better understanding of the epidemiology of envenoming would improve care, provided that the survey is representative, reliable and accurate. Several types of surveys could help to clarify the incidence, severity, circumstances, factors and determinants of envenomations. The relevant information may be collected and analyzed from hospital records or case report forms (for retrospective studies) or established from a protocol of longitudinal observation of cases attended at health facilities during a given period (prospective study). The household survey includes interviewing all or part of the population of a locality with a standardized questionnaire to obtain information on the circumstances of the accident. Finally, standardized questionnaires can be...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Epidemiology; Snakebite; Methodology; Retrospective surveys; Prospective surveys; Household surveys.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400014

Serological profile of anti-Toxoplasma gondii antibodies in apparently healthy dogs of the city of Botucatu, São Paulo State, Brazil
Provedor de dados: 80
Autores: Langoni,H.; Modolo,J. R.; Pezerico,S. B.; Silva,R. C.; Castro,A. P. B.; Silva,A. V. da; Padovani,C. R.
Toxoplasmosis is a zoonosis caused by Toxoplasma gondii, a coccidian protozoan of worldwide distribution. The seroprevalence in canine population can be an alternative for measuring T. gondii urban spreading. A total of 780 blood samples from dogs were collected, during the yearly anti-rabies campaign, carried out by the Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Husbandry (FMVZ), São Paulo State University, UNESP, together with the county health authorities, in August 1999. Using Indirect Fluorescent Antibody Test (IFAT) for detecting antibodies anti-T. gondii in the sera samples, we observed that 258 dogs (33.1%) were positive. The associations between the serological results and the epidemiological...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Toxoplasma gondii; Dog; Serology.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100012

Effects of Bothrops moojeni venom on Leishmania amazonensis promastigote forms
Provedor de dados: 80
Autores: Castilhos,P; Pereira,CG; Silva,ALN; Napolitano,DR; Oliveira,F; Souza,MA.
Tipo: Info:eu-repo/semantics/article
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200005

Spider venom peptides as potential drug candidates due to their anticancer and antinociceptive activities
Provedor de dados: 80
Autores: Wu,Ting; Wang,Meng; Wu,Wenfang; Luo,Qianxuan; Jiang,Liping; Tao,Huai; Deng,Meichun.
Abstract Spider venoms are known to contain proteins and polypeptides that perform various functions including antimicrobial, neurotoxic, analgesic, cytotoxic, necrotic, and hemagglutinic activities. Currently, several classes of natural molecules from spider venoms are potential sources of chemotherapeutics against tumor cells. Some of the spider peptide toxins produce lethal effects on tumor cells by regulating the cell cycle, activating caspase pathway or inactivating mitochondria. Some of them also target the various types of ion channels (including voltage-gated calcium channels, voltage-gated sodium channels, and acid-sensing ion channels) among other pain-related targets. Herein we review the structure and pharmacology of spider-venom peptides that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spider venom peptides; Antitumor; Pain; Drug candidates.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100203

Resistance of cervical adenocarcinoma cells (HeLa) to venom from the scorpion Centruroides limpidus limpidus
Provedor de dados: 80
Autores: Contreras-Ortiz,José María Eloy; Vázquez-Chagoyán,Juan Carlos; Martínez-Castañeda,José Simón; Estrada-Franco,José Guillermo; Aparicio-Burgos,José Esteban; Acosta-Dibarrat,Jorge; Barbabosa-Pliego,Alberto.
Background : The venom of Centruroides limpidus limpidus (Cll) is a mixture of pharmacologically active principles. The most important of these are toxic proteins that interact both selectively and specifically with different cellular targets such as ion channels. Recently, anticancer properties of the venom from other scorpion species have been described. Studies in vitro have shown that scorpion venom induces cell death, inhibits proliferation and triggers the apoptotic pathway in different cancer cell lines. Herein, after treating human cervical adenocarcinoma (HeLa) cells with Cll crude venom, their cytotoxic activity and apoptosis induction were assessed. Results : Cll crude venom induced cell death in normal macrophages in a dose-dependent manner....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venoms; Cytotoxic tests; Apoptosis; HeLa cells; Anticancer agents; Centruroides toxins.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100311

Eating habits: are we safe to consume freshwater puffer fish from the Amazon region in Brazil?
Provedor de dados: 80
Autores: Freitas,José Carlos de.
Tipo: Info:eu-repo/semantics/other
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200001

Snake envenomations in northwest counties of the Rio de Janeiro State, Brazil
Provedor de dados: 80
Autores: Tinoco,H. B.; Norberg,A. N.; Pile,E.; Carvalho,C. R. P.; Silva,D. A.; Guerra-Sanches,F.
The frequency of envenoming in Northwest counties of the Rio de Janeiro State, Brazil, from 1995 to 2000 was evaluated. Reports from the Municipal Secretariat of Health of these counties were used. The results demonstrated that, from 1997 to 1999, there was a shortage of notification, and 40 cases of envenomations caused by Bothrops snakes were registered. These cases were more common from February to October, and the lower limbs of male peasants were the mainly affected areas.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Poisonous snakes; Bothrops; Envenomations; Rio de Janeiro; Brazil.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100005

Malignant catarrhal fever-like lesions associated with ovine herpesvirus-2 infection in young calves (Bos indicus): a case report
Provedor de dados: 80
Autores: Luvizotto,MCR; Ferrari,HF; Cardoso,TC.
Infection of susceptible ruminants, including domestic cattle (Bos taurus) and American bison (Bison bison), with ovine herpesvirus-2 (OvHV-2) may provoke the fatal vasculitis and lymphoproliferative syndrome, known as malignant catarrhal fever (MCF), reported worldwide. To the best of our knowledge, this is the first report of a clinical case of MCF-like lesions associated with ovine herpesvirus-2 (OvHV-2) infection in young calves (Bos indicus) including central nervous symptoms that occurred in Três Lagoas city, Mato Grosso do Sul state, a border town near São Paulo state, Brazil. The diagnosis was based on typical histological lesions characterized by systemic lymphohistiocytic and fibrinoid vasculitis, confirmed by polymerase chain reaction and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovine herpesvirus-2; Malignant catarrhal fever (MCF); Meningoencephalitis; Cattle.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100018

Immunopathology of rabies infection in mice selected for high or low acute inflammatory reaction
Provedor de dados: 80
Autores: Achkar,S. M.; Sinhorini,I. L.; Ribeiro,O. G.; Carrieri,M. L.; Ceretta,R. S.; Consales,C. A.
Rabies is a severe and lethal disease that produces a slight inflammatory response during the infection process. We analyzed the immunopathological mechanisms that occur in the central nervous system (CNS) using mice genetically selected for maximal or minimal acute inflammatory reaction (AIRmax or AIRmin). As viral samples, we adopted the antigenic variant 3 (AgV3) of rabies virus from hematophagous bats and a fixed virus strain (PV1 43/3). Titration of specific antibodies was performed using enzyme-linked immunosorbent assay (ELISA). We observed a slight increase in IgG and IgG1 isotypes in infected AIRmax mice. Incubation period, determined by intracerebral inoculation with 100 LD50, was 6-7 days for PV1 43/4 strain and 9-10 days for AgV3. No difference...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Virus replication; Pathology; Inflammation; Immunoglobulin isotypes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100003

Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens
Provedor de dados: 80
Autores: Richini-Pereira,VB; Bosco,SMG; Theodoro,RC; Barrozo,L; Bagagli,E.
Road-killed wild animals have been for years used for surveillance of vectors of zoonotic pathogens and may offer new opportunities for eco-epidemiological studies. In the current study, fungal infection was evaluated by PCR and nested-PCR in tissue samples collected from 19 road-killed wild animals. The necropsies were carried out and samples were collected for DNA extraction. Results, using PCR with a panfungal primer and nested PCR with specific primers, indicated that some animals are naturally infected with Amauroascus aureus, Metarhizium anisopliae, Aspergillus flavus, Aspergillus oryzae, Emmonsia parva, Paracoccidioides brasiliensis or Pichia stipitis. The approach employed herein proved useful for detecting the environmental occurrence of several...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Road-killed animals; Fungal pathogens; Eco-epidemiology; PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400011

The venom gland of the scorpion species Euscorpius mingrelicus (Scorpiones: Euscorpiidae): morphological and ultrastructural characterization
Provedor de dados: 80
Autores: Yigit,N; Benli,M.
The histology and ultrastructure of venom glands in the scorpion Euscorpius mingrelicus (Kessler, 1874) are described and illustrated in the current study for the first time by employing light microscopy and transmission electron microscopy (TEM). The venom apparatus is composed of a pair of venom glands and a stinger, both situated in the last segment of the metasoma. The venom glands are completely separate but similar. The two glands are segregated within the telson by striated muscle bundles, and their outer surfaces are surrounded by a cuticle. An internal layer constitutes the secretory epithelium. This epithelium is made up of simple columnar cells. The nucleus and organelles involved in cellular synthetic activity are situated basally. In the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Euscorpius mingrelicus; Scorpion; Telson; Venom gland; Histology; Ultrastructure.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300007

Detection of cantharidin-related compounds in Mylabris impressa (Coleoptera: Meloidae)
Provedor de dados: 80
Autores: Nikbakhtzadeh,M. R.; Ebramihi,B.
Cantharidin is mainly found in the beetle families Meloidae and Oedemeridae (Insecta: Coleoptera) which are the natural producers of this terpene anhydride. Most studies to date have focused on cantharidin distribution in blister beetles, with few reports on recently found cantharidin-related compounds (CRCs). Using gas chromatography-mass spectrometry (GC-MS), the present work reported cantharidin and two CRCs, palasonin and cantharidinimide from Mylabris impressa stillata (Baudi, 1878) which was collected from Toyserkan county, Hamedan Province, Iran. Ionization provided mass spectra with characteristic fragments of cantharidin at m/z 96 and 128, demethylcantharidin at m/z 82 and 114, and cantharidinimide at m/z 70, 96 and 127. This is the first time...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cantharidin; CRC; Palasonin; Cantharidinimide; Blistering beetle; Meloidae; Mylabris.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300011

Antileishmanial and antitrypanosomal activity of the cutaneous secretion of Siphonops annulatus
Provedor de dados: 80
Autores: Pinto,Erika Gracielle; Antoniazzi,Marta Maria; Jared,Carlos; Tempone,and Andre Gustavo.
Background Among the tropical parasitic diseases, those caused by protozoans are considered a challenge to public health, being represented by leishmaniasis and Chagas disease. In view of the low effectiveness and toxicity of the current therapy, animal venoms such as amphibian secretions have been used as a promising source of new drug prototypes. The present work aimed to achieve bioguided fractionation of metabolites present in a cutaneous secretion of the caecilian Siphonops annulatus (Amphibia: Gymnophiona: Siphonopidae) with antileishmanial and antitrypanosomal activity.Methods Through liquid-liquid partition and chromatographic techniques, the secretion was fractionated using bioguided assays. The 50% inhibitory concentration (IC50) of the main...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amphibians; Venoms; Leishmania; Trypanosoma cruzi; Therapy; Drugs.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200340

Articular inflammation induced by an enzymatically-inactive Lys49 phospholipase A2: activation of endogenous phospholipases contributes to the pronociceptive effect
Provedor de dados: 80
Autores: Dias,Renata Gonçalves; Sampaio,Sandra Coccuzzo; Sant’Anna,Morena Brazil; Cunha,Fernando Queiroz; Gutiérrez,José María; Lomonte,Bruno; Cury,Yara; Picolo,Gisele.
Abstract  Background  Arthritis is a set of inflammatory conditions that induce aching, stiffness, swelling, pain and may cause functional disability with severe consequences to the patient’s lives. These are multi-mediated pathologies that cannot be effectively protected and/or treated. Therefore, the aim of this study was to establish a new model of acute arthritis, using a Lys49-PLA2 (Bothrops asper myotoxin II; MT-II) to induce articular inflammation.  Methods  The articular inflammation was induced by MT-II (10 μg/joint) injection into the left tibio-tarsal or femoral-tibial-patellar joints. Cellular influx was evaluated counting total and differential cells that migrated to the joint. The plasma extravasation was determined using Evans blue dye. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lys49-PLA2; Myotoxin II; Arthritis; Bothrops asper; Phospholipase.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100307

Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells
Provedor de dados: 80
Autores: Monroy-Estrada,Heidi I; Chirino,Yolanda I; Soria-Mercado,Irma E; Sánchez-Rodríguez,Judith.
Background Lung cancer causes 1.4 million deaths worldwide while non-small-cell lung cancer (NSCLC) represents 80-85% of the cases. Cisplatin is a standard chemotherapy against this type of cancer; however, tumor cell resistance to this drug limits its efficacy. Sea anemones produce compounds with pharmacological activities that may be useful for augmenting cisplatin efficacy. This study aimed to evaluate the pharmacological activities of crude venom (CV) from the sea anemone Bunodeopsis globulifera and four derived fractions (F1, F2, F3 and F4) to test their increase efficiency cisplatin cytotoxicity in human lung adenocarcinoma cells. Results Pre-exposure to CV, F1 and F2 fractions increases cisplatin cytotoxicity in human lung adenocarcinoma cells under...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cnidaria; Pharmacology; Human lung cancer cells; Cytotoxicity assay; Cisplatin efficacy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100308

The International Congress of Toxinology in Brasil: a brief overview
Provedor de dados: 80
Autores: De Lima,ME; Fortes-Dias,CL.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200001

Necrotic skin lesion in a dog attributed to Loxosceles (brown spider) bite: a case report
Provedor de dados: 80
Autores: Machado,LHA; Antunes,MIPP; Mazini,AM; Sakate,M; Torres-Neto,R; Fabris,VE; Vailati,MCF; Lourenço,MLG.
Envenomations caused by Loxosceles (brown spider) have been reported throughout the world. Clinical signs associated to bites of these spiders involve dermonecrotic lesions and intense local inflammatory response, besides systemic manifestations such as intravascular hemolysis, thrombocytopenia, disseminated intravascular coagulation and acute renal failure. The present study aimed to report and to describe dermonecrotic lesions probably caused by a Loxosceles envenomation in a four year-old poodle female dog, treated at the Dermatology Service of the Veterinary Hospital of the Veterinary Medicine and Animal Husbandry School, São Paulo State University, Botucatu, Brazil. Initially, the animal presented two skin lesions with blackish aspect that evolved...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Loxosceles; Dermonecrotic toxin; Dogs.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300017

Identification of bradykinin: related peptides from Phyllomedusa nordestina skin secretion using electrospray ionization tandem mass spectrometry after a single-step liquid chromatography
Provedor de dados: 80
Autores: Conceição,K; Bruni,FM; Sciani,JM; Konno,K; Melo,RL; Antoniazzi,MM; Jared,C; Lopes-Ferreira,M; Pimenta,DC.
Amphibian skin secretions are a source of potential new drugs with medical and biotechnological applications. Rich in peptides produced by holocrine-type serous glands in the integument, these secretions play different roles, either in the regulation of physiological skin functions or in the defense against predators or microorganisms. The aim of the present work was to identify novel peptides with bradykinin-like structure and/or activity present in the skin of Phyllomedusa nordestina. In order to achieve this goal, the crude skin secretion of this frog was pre-fractionated by solid phase extraction and separated by reversed-phase chromatography. The fractions were screened for low-molecular-mass peptides and sequenced by mass spectrometry. It was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phyllomedusa nordestina; Bradykinin; Mass spectrometry; Natural peptides.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400004

Vasoconstrictor effect of Africanized honeybee (Apis mellifera L.) venom on rat aorta
Provedor de dados: 80
Autores: Sousa,Paulo César P; Brito,Teresinha S; Freire,Daniel S; Ximenes,Rafael M; Magalhães,Pedro Jorge C; Monteiro,Helena SA; Alves,Renata S; Martins,Alice Maria C; Toyama,Daniela O; Toyama,Marcos H.
Background : Apis mellifera stings are a problem for public health worldwide, particularly in Latin America due to the aggressiveness of its Africanized honeybees. Massive poisoning by A. mellifera venom (AmV) affects mainly the cardiovascular system, and several works have described its actions on heart muscle. Nevertheless, no work on the pharmacological action mechanisms of the AmV in isolated aorta has been reported. Thus, the present work aimed to investigate the actions of AmV and its main fractions, phospholipase A2 (PLA2) and melittin, on isolated aorta rings and a probable action mechanism. Results : AmV and the complex PLA2 + melittin (0.1-50 μg/mL) caused contraction in endothelium-containing aorta rings, but neither isolated PLA2 nor melittin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apis mellifera; Venom; Mellitin; Phospholipase A2; Aorta.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100314

Microhabitat use by species of the genera Bothrops and Crotalus (Viperidae) in semi-extensive captivity
Provedor de dados: 80
Autores: Gomes,CA; Almeida-Santos,SM.
Many factors influence microhabitat including climate and the occurrence of predators, prey and suitable shelters. The influence of predators in a semi-extensive breeding system is minimized due to frequent monitoring of the area. This situation enables the independent analysis of such other variables as refuges and temperature. Some specimens of the Viperidae family are kept in a semi-extensive breeding system at the Butantan Institute for display and study. These animals are widely distributed in the Atlantic Forest and Cerrado, two biomes with distinct climatic characteristics. We compared the daily activity pattern and microhabitat use of the species Bothrops jararaca and Crotalus durissus terrificus. Our main questions were whether rattlesnakes and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microhabitat selection; Snakes; Thermoregulation; Viperidae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400007

Paracoccidioidomycosis: advances and unmet needs 100 years after its initial description by Lutz
Provedor de dados: 80
Autores: Colombo,Arnaldo Lopes.
Tipo: Info:eu-repo/semantics/other
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300001

Pharmacological characterization of rat paw edema induced by Naja haje arabica venom
Provedor de dados: 80
Autores: Al-Asmari,A. K.
This investigation was performed in order to assess the inflammatory response induced by Naja haje arabica venom (NhaV) in rat hind paw. The inflammatory response was estimated by measuring the edema with a Plethysmometer. The venom (0.625-10mug/paw) produced a dose and time-dependent increase in non-hemorrhagic paw edema. The response to NhaV was maximal within 15 min and disappeared in 24 h. Five mug/paw of NhaV was chosen to test the effect of various drugs on the edema induced by this venom. Quinacrine (QNC), a phospholipase A2 (PLA2) inhibitor, and dipyridamole (DPM), an adenosine transport inhibitor, attenuated venom-induced edema in rat paw (P&lt;0.001). Commercially available antivenom was ineffective when administered intravenously, whereas its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat paw edema; Naja haje arabica venom; Inflammatory mediators; Inhibitor; Antivenom.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100007

Peptidomic investigation of Neoponera villosa venom by high-resolution mass spectrometry: seasonal and nesting habitat variations
Provedor de dados: 80
Autores: Cologna,Camila Takeno; Rodrigues,Renata Santos; Santos,Jean; Pauw,Edwin de; Arantes,Eliane Candiani; Quinton,Loïc.
Abstract  Background: Advancements in proteomics, including the technological improvement in instrumentation, have turned mass spectrometry into an indispensable tool in the study of venoms and toxins. In addition, the advance of nanoscale liquid chromatography coupled to nanoelectrospray mass spectrometry allows, due to its high sensitivity, the study of venoms from species previously left aside, such as ants. Ant venoms are a complex mixture of compounds used for defense, predation or communication purposes. The venom from Neoponera ants, a genus restricted to Neotropical regions, is known to have cytolytic, hemolytic, antimicrobial and insecticidal activities. Moreover, venoms from several Neoponera species have been compared and differences in their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ant venom; Peptidomics; Ponericins; Venom comparison; Antimicrobial peptides.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100303

Purification and enzymatic characterization of a novel metalloprotease from Lachesis muta rhombeata snake venom
Provedor de dados: 80
Autores: Cordeiro,Francielle Almeida; Coutinho,Bárbara Marques; Wiezel,Gisele Adriano; Bordon,Karla de Castro Figueiredo; Bregge-Silva,Cristiane; Rosa-Garzon,Nathalia Gonsales; Cabral,Hamilton; Ueberheide,Beatrix; Arantes,Eliane Candiani.
Abstract  Background: Lachesis muta rhombeata (Lmr) is the largest venomous snake in Latin America and its venom contains mainly enzymatic components, such as serine and metalloproteases, L-amino acid oxidase and phospholipases A2. Metalloproteases comprise a large group of zinc-dependent proteases that cleave basement membrane components such as fibronectin, laminin and collagen type IV. These enzymes are responsible for local and systemic changes, including haemorrhage, myonecrosis and inflammation. This study aimed the isolation and enzymatic characterization of the first metalloprotease (Lmr-MP) from Lmr venom (LmrV).  Methods and results: Lmr-MP was purified through two chromatographic steps and submitted to enzymatic characterization. It showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lachesis muta rhombeata; Metalloprotease; Proteases; Snake venom.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100323

Unusual visual loss after snakebite
Provedor de dados: 80
Autores: Tungpakorn,N.
Snakebites are endemic in some parts of Thailand, being associated with several complications. Ocular disturbances are uncommon, except in cases of corneal or conjunctival injury, when the eye is directly exposed to the snake venom. The present study presents a case of combined ophthalmic artery occlusion and transient central retinal artery occlusion with macular ischemia after a Russell's viper bite.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Snakebite; Central retinal artery occlusion; Macular ischemia; Vipera russelli siamensis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300020

Morphological characterization of the venom apparatus in the wolf spider Lycosa singoriensis (Laxmann, 1770)
Provedor de dados: 80
Autores: Yigit,N; Bayram,A; Danisman,T; Sancak,Z; Tel,MG.
The wolf spider Lycosa singoriensis (Laxmann, 1770) (Lycosidae: Araneae) is distributed throughout central and eastern Europe, including Russia, Kazakhistan and Turkey. This study describes the venom apparatus morphology of L. singoriensis through scanning electron microscopy (SEM). Its structure follows the general architecture observed in other spiders. Generally, a venom apparatus is composed by a pair of venom glands and chelicerae. L. singoriensis chelicerae are robust and consist of a stout basis and a movable apical segment (fang). The fang rests in a groove on the basal segment that is covered by different types of hair. L. singoriensis venom glands present equal size and measure about 4 mm in length. Each gland is enclosed by irregular muscular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spider; Lycosa singoriensis; Chelicerae; Venom gland; Morphology.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100013

Envenomation by caterpillars (erucism): proposal for simple pain relief treatment
Provedor de dados: 80
Autores: Haddad,Vidal; Lastória,Joel Carlos.
Tipo: Info:eu-repo/semantics/other
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200102

HTLV-I/II seroprevalence and risk factors in pregnant women treated at primary health units in the municipality of Botucatu
Provedor de dados: 80
Autores: Neto,J. O.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100022

Rhabdomyolysis secondary to an accident with marine stingray (Dasyatis family)
Provedor de dados: 80
Autores: Masson,AA; Ormonde do Carmo,PHA; Carvalho,JLV.
Whenever humans come in touch with the sea, they become vulnerable to risks, most frequently on account of invading a habitat that belongs to potentially harmful aquatic animals. World literature shows a growing number of incidents in recent years with marine stingrays, despite the fact that these animals only attack when harassed. This report describes an accident in which an amateur fisherman was injured by a marine stingray, probably of the Dasyatis family, on his left forearm. The puncture wound was highly painful and progressed to rhabdomyolysis. It is conjectured that lymphatic drainage, applied in an attempt to reduce edema and its complications, may have contributed to rebound increase in serum creatine kinase levels (CK) and, thus, has exacerbated...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Stingray injury; Lymphatic drainage; Rhabdomyolysis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300013

Mathematical modelling of vector-borne diseases and insecticide resistance evolution
Provedor de dados: 80
Autores: Kuniyoshi,Maria Laura Gabriel; Santos,Fernando Luiz Pio dos.
Abstract  Background: Vector-borne diseases are important public health issues and, consequently, in silico models that simulate them can be useful. The susceptible-infected-recovered (SIR) model simulates the population dynamics of an epidemic and can be easily adapted to vector-borne diseases, whereas the Hardy-Weinberg model simulates allele frequencies and can be used to study insecticide resistance evolution. The aim of the present study is to develop a coupled system that unifies both models, therefore enabling the analysis of the effects of vector population genetics on the population dynamics of an epidemic.  Methods: Our model consists of an ordinary differential equation system. We considered the populations of susceptible, infected and recovered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epidemiology; Population genetics; Tropical diseases; Insecticides; Theoretical modelling; Numerical simulation; ODE system.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100321

Impact of malnutrition on immunity and infection
Provedor de dados: 80
Autores: França,TGD; Ishikawa,LLW; Zorzella-Pezavento,SFG; Chiuso-Minicucci,F; da Cunha,MLRS; Sartori,A.
Malnutrition may be a consequence of energy deficit or micronutrient deficiency. It is considered the most relevant risk factor for illness and death, particularly in developing countries. In this review we described the magnitude of this problem, as well as its direct effect on the immune system and how it results in higher susceptibility to infections. A special emphasis was given to experimental models used to investigate the relationship between undernutrition and immunity. Malnutrition is obviously a challenge that must be addressed to health authorities and the scientific community.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malnutrition; Infection; Immunity; Experimental models.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300003

CEVAP Journal: the first Brazilian electronic scientific publication turns 20 years old
Provedor de dados: 80
Autores: Barraviera,Benedito.
Tipo: Info:eu-repo/semantics/other
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100101

Bufotenine, a tryptophan-derived alkaloid, suppresses the symptoms and increases the survival rate of rabies-infected mice: the development of a pharmacological approach for rabies treatment
Provedor de dados: 80
Autores: Vigerelli,Hugo; Sciani,Juliana M.; Pereira,Patricia M. C.; Lavezo,Aline A.; Silva,Andrea C. R.; Collaço,Rita C. O.; Rocha,Thalita; Bueno,Thais C.; Pimenta,Daniel C.
Abstract  Background: Between 40,000-70,000 people die yearly of rabies, an incurable disease. Besides post-bite vaccination, no treatment is available for it.  Methods: First, virus dilution for antiviral effects in mice was determined. Then, animals were treated as follows: control (NaCl 250 µL/animal/day); bufotenine (0.63, 1.05 and 2.1 mg in 250 µL of NaCl/animal/day); rabies (10-6,82CVS dilution); and test (10-6,82 CVS dilution and bufotenine, in the above-mentioned doses). Animals were observed daily for 21 days or until the 3rd stage of rabies infection. Twitch-tension and liposome studies were applied to understand the possible interaction of bufotenine with receptors, particularly acetylcholine.  Results: Bufotenine was able to increase the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Rabies therapy; Bufotenine.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100303

New insights into the structural characteristics of irradiated crotamine
Provedor de dados: 80
Autores: Oliveira,Karina Corleto; Spencer,Patrick Jack; Ferreira Jr,Rui Seabra; Nascimento,Nanci.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gamma radiation; Structural modifications; Crotamine; Snake venom; Crotalus durissus terrificus.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100321

Listeria monocytogenes in HIV-infected patients in a hospital of Nova Iguaçu, Rio de Janeiro, Brazil
Provedor de dados: 80
Autores: Norberg,A. N.; Maure,E. A. P.; Svaiter,N.; Gonçalves,A. S.; Sanches,F. G.
A survey was carried out in a hospital of Nova Iguaçu, Rio de Janeiro, Brazil, in the period from July 1999 to March 2002, to determine the infection rate of Listeriamonocytogenes in HIV+ patients with diarrhea symptoms; 134 samples were processed by microbiological methods. The results demonstrated 12.68% of positive samples. However, no statistical differences were observed for age or sex in the studied group, suggesting that this microorganism should be regarded in the differential diagnosis of infectious processes in HIV+ patients in the area.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeriosis; HIV; Diarrhea.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400016

Tracking false-negative results in molecular diagnosis: proposal of a triplex-PCR based method for leishmaniasis diagnosis
Provedor de dados: 80
Autores: Gonçalves-de-Albuquerque,Suênia da Cunha; Silva,Rômulo Pessoa e; Morais,Rayana Carla Silva de; Trajano-Silva,Lays Adrianne Mendonça; Régis-da-Silva,Carlos Gustavo; Brandão Filho,Sinval Pinto; Paiva-Cavalcanti,Milena de.
Background : Molecular biological methods have become increasingly relevant to the diagnosis and control of infectious diseases, such as leishmaniasis. Since various factors may affect the sensitivity of PCR assays, including DNA yield and purity, an optimal extraction method is pivotal. Losses of a parasite’s DNA during extraction may significantly impair its detection by PCR and lead to false-negative results. This study proposes a triplex PCR assay targeting the parasite’s DNA, an external control (pUC18) and an internal control (G3PD) for accurate diagnosis of leishmaniasis.Results : Two primer pairs were designed to detect the plasmid pUC18 and a triplex PCR assay targeting theLeishmania braziliensiskinetoplast DNA, the external control and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extraction control; Multiplex PCR; PUC18; Leishmaniasis; Diagnosis; False-negative result.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200326

Can anti-bothropstoxin-I antibodies discriminate between Bothrops jararaca and Bothrops jararacussu venoms?
Provedor de dados: 80
Autores: Araujo,Ricardo Teixeira; Corrêa-Netto,Carlos; Brazil-Más,Leonora; Silveira,Caio Raony Farina; Fernandes,Irene; Zingali,Russolina Benedeta.
Abstract  Background  Snakes of the genus Bothrops, popularly known as pit vipers, are responsible for most cases of snakebite in Brazil. Within this genus, Bothrops jararacussu and B. jararaca deserve special attention due to the severity of their bites and for inhabiting densely populated areas. Regarding the treatment of snakebites by Bothrops jararacussu, questions have been raised about the effectiveness of the specific bothropic antivenom in neutralizing myotoxic effects; however, there are no accurate data for humans. Thus, the development of a differential diagnostic kit for this species would be of great interest because it provides, for healthcare professionals, a tool that would allow us to determine whether the accident was caused by B....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararacussu; Bothrops jararaca; Bothropstoxin-I; Monoclonal antibodies; Species specificity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100308

Effect of Bothrops alternatus snake venom on macrophage phagocytosis and superoxide production: participation of protein kinase C
Provedor de dados: 80
Autores: Setubal,SS; Pontes,AS; Furtado,JL; Kayano,AM; Stábeli,RG; Zuliani,JP.
Envenomations caused by different species of Bothrops snakes result in severe local tissue damage, hemorrhage, pain, myonecrosis, and inflammation with a significant leukocyte accumulation at the bite site. However, the activation state of leukocytes is still unclear. According to clinical cases and experimental work, the local effects observed in envenenomation by Bothrops alternatus are mainly the appearance of edema, hemorrhage, and necrosis. In this study we investigated the ability of Bothrops alternatus crude venom to induce macrophage activation. At 6 to 100 ¼g/mL, BaV is not toxic to thioglycollate-elicited macrophages; at 3 and 6 ¼g/mL, it did not interfere in macrophage adhesion or detachment. Moreover, at concentrations of 1.5, 3, and 6 ¼g/mL...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops; Macrophages; Phagocytosis; Superoxide; Protein kinase C; Inflammation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400010

Excitatory effects of Buthus C56 toxin on Drosophila larval neuromuscular junction
Provedor de dados: 80
Autores: Gawade,S. P.
Buthus C56 toxin from venom of the Indian red scorpion Mesobuthus tamulus was studied for its effects on spontaneous miniature excitatory junctional potentials (MEJP) on Drosophila larval neuromuscular junctions. C56 toxin was isolated on CM-Cellulose with linear gradient of ammonium acetate buffer, pH 6.0. Toxin purity was determined on SDS slab gel electrophoresis. Effective concentration of C56 toxin was based on contraction paralysis units (CPU) in Drosophila 3rd instar larvae by microinjection (0.1 CPU/ml = 2 x 10-6 g/ml). The toxin-induced excitatory junctional potentials were studied for calcium dependency (0.2 mM to 1.2 mM Ca2+) in Drosophila Ringer. Excitatory junctional potential amplitude was increased with increasing calcium concentration;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesobuthus tamulus; C56 toxin; Drosophila melanogaster; Neuromuscular; Miniature excitatory junctional potentials (MEJPs).
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100004

Endogenous phospholipase A2 inhibitors in snakes: a brief overview
Provedor de dados: 80
Autores: Campos,Patrícia Cota; Melo,Lutiana Amaral de; Dias,Gabriel Latorre Fortes; Fortes-Dias,Consuelo Latorre.
Abstract The blood plasma of numerous snake species naturally comprises endogenous phospholipase A2 inhibitors, which primarily neutralize toxic phospholipases A2 that may eventually reach their circulation. This inhibitor type is generally known as snake blood phospholipase A2 inhibitors (sbPLIs). Most, if not all sbPLIs are oligomeric glycosylated proteins, although the carbohydrate moiety may not be essential for PLA2 inhibition in every case. The presently known sbPLIs belong to one of three structural classes – namely sbαPLI, sbβPLI or sbγPLI – depending on the presence of characteristic C-type lectin-like domains, leucine-rich repeats or three-finger motifs, respectively. Currently, the most numerous inhibitors described in the literature are sbαPLIs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PLA2 inhibitor; Phospholipase A2; Snake blood; Natural resistance; Snakes.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100204

Antimicrobial activity of an L-amino acid oxidase isolated from Bothrops leucurus snake venom
Provedor de dados: 80
Autores: Torres,AFC; Dantas,RT; Menezes,RRPPB; Toyama,MH; Filho,ED; Oliveira,MF; Nogueira,NAP; Oliveira,MR; Monteiro,HSA; Martins,AMC.
Some snake venom proteins present enzymatic activities, such as L-amino acid oxidase (LAAO). The aim of this paper was to investigate the effect of Bothrops leucurus total venom (BleuTV) and its fraction LAAO (BleuLAAO) on bacteria, yeast, and promastigote forms of Leishmania amazonensis and Leishmania chagasi, and epimastigote forms of Trypanosoma cruzi. BleuTV was isolated with a Protein Pack 5PW® (Waters Corporation, USA), and several fractions were obtained. BleuLAAO was purified to high molecular homogeneity, and its N-terminal amino acid sequence shared a high degree of amino acid conservation with other LAAOs. BleuTV inhibited Staphylococcus aureus growth in a dose-dependent manner, with a minimum inhibitory concentration (MIC) of 25 μg/mL, which...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-amino oxidase; Bothrops leucurus; Antimicrobial activity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400012

Serum electrolyte changes in pediatric patients stung by scorpions
Provedor de dados: 80
Autores: Osnaya-Romero,N.; Hernández,T. J. M.; Basurto,G.; Andrade,S.; Figueroa,J. M.; Carvajal,Y.; Flores-Hernandez,S. S.
Scorpion sting is a health problem in some places of Mexico. The clinical manifestations of scorpion envenomation are variable and include metabolic alterations. Hyperkalemia is the most frequently reported metabolic alteration. We conducted a prospective, observational, descriptive and transversal study in an emergency room at Hospital del Niño Morelense, Mexico. Eighty-two patients were included and classified as mild (17%) moderate (33%) and severe (46%). The mean serum level of sodium was 146.4meq/l, standard deviation (SD) 5.58; potassium 3.86meq/l, SD 0.53, and calcium 9.55mg/dl, SD 0.76. We found 30.4% hypernatremia, 12% hypokalemia, 10.9% abdominal distension, and 14.6% visual alterations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serum electrolytes; Scorpion stung children.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200014

Gene polymorphism of interleukin 1 and 8 in chronic gastritis patients infected withHelicobacter pylori
Provedor de dados: 80
Autores: Caleman Neto,Agostinho; Rasmussen,Lucas T; Labio,Roger W de; Queiroz,Valdeir F de; Smith,Marília de AC; Viani,Gustavo A; Payão,Spencer LM.
Background : Epidemiological investigations have indicated thatHelicobacter pyloriinduces inflammation in the gastric mucosa regulated by several interleukins. The genesIL1BandIL8are suggested as key factors in determining the risk of gastritis. The aim of this paper was to evaluate the association of gene polymorphism of interleukin-1 and interleukin-8 with chronic gastrits inH. pyloriinfected patients. A total of 60 patients underwent endoscopic procedure. Biopsy samples were collected for urease test, histopathological and molecular exams. The DNA of theses samples was extracted for detection ofH. pyloriand analysis of the genes mentioned above. Patients with gastritis had a higher frequency ofH. pylori-positive samples.Results : H. pyloriwas detected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Polymorphism; Interleukin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200327

Effect of soluble fiber on hypertriglyceridemia and immune profile in HIV-positive individuals undergoing highly active antiretroviral therapy
Provedor de dados: 80
Autores: Geraix,J.
Tipo: Info:eu-repo/semantics/other
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200016

Maintaining rear-fanged snakes for venom production: an evaluation of mortality and survival rates for Philodryas olfersii and P. patagoniensis in captivity
Provedor de dados: 80
Autores: Braz,HB; Rocha,MMT; Furtado,MFD.
This study evaluates the mortality and average survival rates of captive female Philodryas olfersii and Philodryas patagoniensis snakes maintained for venom production. Also, two factors likely to reduce captive survival were studied - body condition at admission and seasonality. Mortality peaks occurred during the second month in captivity. More than half the individuals were dead at the end of the third month. This suggests that the first three months in captivity are the most critical in terms of survival and adaptation. Females collected and admitted during spring and summer lived less time than those collected in autumn and winter. As gravidity and egg-laying occur during spring and summer, we suggest that the lower survival rates in these seasons may...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal husbandry; Snake venom; Seasonal variation; Body mass index.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200006

Insects as biological models to assay spider and scorpion venom toxicity
Provedor de dados: 80
Autores: Manzoli-Palma,M. F.; Gobbi,N.; Palma,M. S.
This study was undertaken to develop an experimental protocol using insects as biological models to assay venom toxicity of the following spiders Loxosceles gaucho, Phoneutria nigriventer, Nephilengys cruentata and Tityus serrulatus scorpion. Three different insect species were bioassayed: Apis mellifera (Hymenoptera), Grillus assimilis (Orthoptera), and Diatraea saccharalis (Lepidoptera). Venoms were injected into the hemocele of insects with a microsyringe at concentrations that caused dose/weight-dependent effects; doses causing either paralysis (ED50) or death (LD50) were recorded for each venom and insect test-species. T. serrulatus and L. gaucho venoms were lethal to all tested species, while P. nigriventer venom caused paralysis and death, and N....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioassay; Venom; Insect model; Toxicity; Apis mellifera.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200004

Loxosceles spider bite in Turkey (Loxosceles rufescens, Sicariidae, Araneae)
Provedor de dados: 80
Autores: Yigit,N.; Bayram,A.; Ulasoglu,D.; Danisman,T.; Corak Ocal,I.; Sancak,Z.
Loxoscelism is caused by envenomation by spiders that belong to the Loxosceles genus. In loxoscelism, a local necrotic lesion appears and, in many cases, loxoscelism or necrotic araneism is considered a serious public health problem. There is no diagnostic test available to help the physician make a diagnostic or therapeutic decision. Here, we report the case of a severe dermonecrotic araneism (loxoscelism) in Turkey probably due to the bite of Loxosceles rufescens. There was little erythema at the beginning, followed by severe necrosis after 20 days, and skin grafting was needed although the case was treated.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spider bite; Loxoscelism; Skin grafting; Loxosceles rufescens; Turkey.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100016

Infiltrated plaques resulting from an injury caused by the common octopus (Octopus vulgaris): a case report
Provedor de dados: 80
Autores: Haddad Jr,Vidal; Magalhães,Claudia Alves de.
Several species of octopus are considered venomous due to toxins present in the glands connected to their “beak”, which may be associated with hunt and kill of prey. Herein, we report an accident involving a common octopus (Octopus vulgaris) that injured an instructor during a practical biology lesson and provoked an inflamed infiltrated plaque on the hand of the victim. The lesion was present for about three weeks and was treated with cold compresses and anti-inflammatory drugs. It was healed ten days after leaving a hyperchromic macule at the bite site. The probable cause of the severe inflammation was the digestive enzymes of the glands and not the neurotoxins of the venom.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Octopuses; Venomous animals; Aquatic animals; Venoms; Poisoning; Digestive enzymes.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200508

East1 toxin and its presence in a changing microbial world
Provedor de dados: 80
Autores: Sousa,C. P.
This review shows the structure, mode of action, and actual epidemiological data about EAST1 toxin. It is a particularly intriguing bacterial toxin that may subvert multiple cellular processes to yield intestinal epithelial cell secretion. EAST1 toxin was first described in strains of EAggEC that were associated with persistent diarrhea primarily in developing world countries. Molecular organization, mobility, and data in literature are suggesting that EAST1 could be a transposon. The insertion sequences in Escherichia coli and some of the usual transposition mechanisms as well as regulation are reviewed. This review emphasizes the presence of the gene astA in EPEC, EAggEC, A-EPEC, ETEC, DAEC, EIEC, and in non-diarrheagenic E. coli. It also discusses here...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EAST1 toxin; AstA gene; Virulence in Escherichia coli; Virulence in Salmonella spp.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100002

Biochemical and biological properties of Lonomia obliqua bristle extract
Provedor de dados: 80
Autores: Chudzinski-Tavassi,A. M.; Carrijo-Carvalho,L. C.
Lonomia obliqua caterpillar is frequently seen in accidents with humans especially in the south of Brazil. Patients develop a hemorrhagic syndrome that can be treated with specific antilonomic serum. A consumptive coagulopathy was found to be the main cause of bleeding complications observed in patients after contact with L. obliqua. Studies revealed that L. obliqua caterpillar bristle extract (LOCBE) displays a procoagulant activity that leads to intravascular thrombin formation, resulting in a special form of disseminated intravascular coagulation (DIC). Fibrinolysis seems to be secondary to the fibrin production, since no direct fibrinolytic activity was found in LOCBE. Two procoagulant toxins, a factor X activator (Losac) and a prothrombin activator...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lonomia obliqua; Lonomia obliqua bristle extract; Hemorrhagic syndrome; Disseminated intravascular coagulation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200002

Cardiotoxic effects of venoms from Chironex fleckeri and Chiropsalmus sp. on an invertebrate model
Provedor de dados: 80
Autores: Carrette,T.; Seymour,J.
Extracted venoms from two species of cubozoan, Chironex fleckeri (C. fleckeri)and Chiropsalmus sp., were injected into the freshwater crayfish Cherax quadricarinatus (C. quadricarinatus). By means of a Vascular Doppler, the cardiac parameters heart rate and contraction amplitude were recorded, with the theoretical total heart output calculated from these two recordings. Cherax quadricarinatus reflects prey items utilized by these two species in the wild and so comparisons of venom action on this animal have greater ecological relevance than those of previous mammalian models used. While the results of this study reflect previous research showing that the overall potency of C. fleckeri venom is greater than that of Chiropsalmus sp. venom, the action of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cubozoan; Venom; Cardiac function; Cherax quadricarinatus; Chironex fleckeri; Chiropsalmus sp.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200007

Anaphylactic shock following the bite of a wild Kayan slow loris (Nycticebus kayan): implications for slow loris conservation
Provedor de dados: 80
Autores: Madani,George; Nekaris,K Anne-Isola.
Background Asian slow lorises (Nycticebus spp.) are one of few known venomous mammals, yet until now only one published case report has documented the impact of their venomous bite on humans. We describe the reaction of a patient to the bite of a subadult Nycticebus kayan, which occurred in the Mulu District of Sarawak in 2012.Findings Within minutes of the bite, the patient experienced paraesthesia in the right side of the jaw, ear and right foot. By 40 minutes, swelling of the face was pronounced. The patient was admitted to Mulu National Park Health Clinic/Klinik Kesihatan Taman Mulu Tarikh, at which time he was experiencing: swollen mouth, chest pain, mild abdominal pain, nausea, numbness of the lips and mouth, shortness of breath, weakness, agitation...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Anaphylaxis; Hypersensitivity; Systemic reaction; Malaysia; Adrenaline; Necrosis; Paresthesia; Animal bite; Mammal venom; Hematuria.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200604

Cryptosporidium SP in HIV-infected individuals attending a Brazilian University Hospital
Provedor de dados: 80
Autores: Langoni-Júnior,G.; Souza,L. R.
The aim of the current work was to evaluate the occurrence of Cryptosporidium sp in AIDS patients in a region of São Paulo State, Brazil. Patients were divided into groups according to CD4+ T lymphocyte count and use of potent antiretroviral treatment. Two hundred and ten fecal samples from 105 patients were fixed in 10% formalin and subjected to centrifuge formol-ether sedimentation. Slides were stained with auramine and confirmed by modified Ziehl-Neelsen. Cryptosporidiosis occurrence was 10.5% with no relationship among gender, age or the presence of diarrhea. The number of oocysts in all samples was small, independent of CD4+ T lymphocyte count, HIV plasma viral load, and presence of diarrhea. These results may be due to the reduced prevalence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Viral load; HIV; HAART; Cryptosporidium; CD4-positive T-lymphocytes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400005

A novel lipocalin homologue from the venom gland of Deinagkistrodon acutus similar to mammalian lipocalins
Provedor de dados: 80
Autores: Wei,CB; Chen,J.
Lipocalins are involved in a variety of functions including retinol transport, cryptic coloration, olfaction, pheromone transport, prostaglandin synthesis, regulation of the immune response and cell homeostatic mediation. A full-length cDNA clone (named d-lipo), isolated from the venom gland cDNA library of Deinagkistrodon acutus, contained an insert of 664 bp including an open reading frame that encodes a lipocalin homologue of 177 amino acids. Comparison of d-lipo and other related proteins revealed an overall amino acid identity of less than 21.5%. Primary structures of d-lipo carried three structurally conserved regions (SCR) showing homologies to those of lipocalins. The first conserved Cys residue - the essential amino acid residue for the catalytic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Deinagkistrodon acutus; CDNA library; Lipocalin; Phylogeny; Gene expression pattern analysis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100003

Envenomation caused by Rhopalurus amazonicus Lourenço, 1986 (Scorpiones, Buthidae) in Pará State, Brazil
Provedor de dados: 80
Autores: Fuentes-Silva,Deyanira; Santos Jr,Alfredo P; Oliveira,Joacir Stolarz.
Scorpions, mainly those belonging to the genus Tityus cause many deaths and injuries in Brazil, with tens of thousands of envenomations notified every year. However, injuries involving other scorpion species are scarcely registered. Among the sixteen species of the genus Rhopalurus, Thorell, 1876, described up to date, nine are found in this country, with only a confirmed case of human envenomation provoked by R. agamemnonKoch, 1839. The present case reports, for the first time, a case of scorpion sting in a human victim involving Rhopalurus amazonicus, endemic species of the west region of the Pará state, Amazon, Brazil. The symptoms of envenomation were local pain and paresthesia. This study contributes to develop the knowledge on venomous scorpions,...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Scorpion sting; Rhopalurus amazonicus; Venomous animals; Envenomation; Brazilian Amazon; Pará.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200509

Seroepidemiological analysis of toxoplasmosis in college students
Provedor de dados: 80
Autores: Rodrigues,Jaqueline Polizeli; Frei,Fernando; Navarro,Italmar Teodorico; Silva,Luciana Pereira; Marcelino,Monica Yonashiro; Andrade-Junior,Heitor Franco de; Faria,Carolina Arruda de; Santos,Marislene; Ribeiro-Paes,João Tadeu.
Background Toxoplasmosis is a zoonosis caused by an obligate intracellular parasite, Toxoplasma gondii, which affects warm-blooded animals including humans. Its prevalence rates usually vary in different regions of the planet. Methods In this study, an analysis of the seroprevalence of toxoplasmosis among Brazilian students was proposed by means of IgG specific antibodies detection. The presence of anti-Toxoplasma gondiiantibodies by indirect fluorescent antibody test (IFAT) was also evaluated in order to compare it with enzyme-linked immunosorbent assay (ELISA) and to assess the use of 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and o-phenylenediamine dihydrochloride chromogens. Results The IFAT method showed a seroprevalence of 22.3%. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Toxoplasmosis; Enzyme-linked immunosorbent assay; Indirect fluorescent antibody test; Seroprevalence; Epidemiology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100311

Immunological studies and in vitro schistosomicide action of new imidazolidine derivatives
Provedor de dados: 80
Autores: JKAL,Neves; S,Sarinho; CML,de Melo; VRA,Pereira; MCA,de Lima; IR,Pitta; MCPA,Albuquerque; SL,Galdino.
Schistosomiasis is a major public health problem with 207 million people infected and more than 779 million at risk. The drug of choice for treating schistosomiasis is praziquantel (PZQ); however, it is inefficient against immature forms of schistosomes. The aim of this study was to test new imidazolidine derivatives LPSF/PT09 and LPSF/PT10 against adult Schistosoma mansoni worms. IC50, cytotoxicity, immune response and cell viability assays were also available for these imidazolidines. Different concentrations of imidazolidine, from 32 to 320 ¼M, promoted motor abnormalities in breeding and unpaired worms, and death in 24 hours at higher concentrations. Although LPSF/PT09 and LPSF/PT10 did not affect IFN-³ and IL-10 production, they induced nitric oxide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schistosoma mansoni; In vitro; Imidazolidines.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300007

Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study
Provedor de dados: 80
Autores: Baldo,C; Ferreira,MJ; Lopes,DS; Izidoro,LFM; Gomes,AO; Ferro,EAV; Hamaguchi,A; Homsi-Brandeburgo,MI; Rodrigues,VM.
The damaging effects of neuwiedase, a non-hemorrhagic snake venom metalloproteinase from P-I class, on gastrocnemius muscle are studied herein. Following neuwiedase injection, ultrastructural alterations were detected early showing disarrangement of skeletal muscle fibers (characterized by discontinuity of Z lines), mitochondrial swelling, and disruption of plasma membrane and basal lamina. Degradation of skeletal muscle and the appearance of an amorphous substance, primarily composed of cellular debris, were noted after 24 hours. The presence of neuwiedase at the injection site (detected by immunocytochemistry) revealed highly specific labeling of myofibril components of damaged myocytes. In addition, proteolysis of muscle proteins assayed through...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Skeletal muscle; Neuwiedase; Snake venom metalloproteinases; Bothrops neuwiedi.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300013

Subproteome of Lachesis muta rhombeata venom and preliminary studies on LmrSP-4, a novel snake venom serine proteinase
Provedor de dados: 80
Autores: Wiezel,Gisele A; Bordon,Karla CF; Silva,Ronivaldo R; Gomes,Mário SR; Cabral,Hamilton; Rodrigues,Veridiana M; Ueberheide,Beatrix; Arantes,Eliane C.
Abstract  Background: Lachesis muta rhombeata is one of the venomous snakes of medical importance in Brazil whose envenoming is characterized by local and systemic effects which may produce even shock and death. Its venom is mainly comprised of serine and metalloproteinases, phospholipases A2 and bradykinin-potentiating peptides. Based on a previously reported fractionation of L. m. rhombeata venom (LmrV), we decided to perform a subproteome analysis of its major fraction and investigated a novel component present in this venom.  Methods: LmrV was fractionated through molecular exclusion chromatography and the main fraction (S5) was submitted to fibrinogenolytic activity assay and fractionated by reversed-phase chromatography. The N-terminal sequences of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bushmaster; Snake venom; SVSP; Kallikrein-like; Plasminogen activator; Kininogenase; Lectin; Protease; Envenomation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100307

The first reported case of human tick paralysis in Brazil: a new induction pattern by immature stages
Provedor de dados: 80
Autores: Almeida,RAMB; Ferreira,MA; Barraviera,B; Haddad Jr,V.
Tick paralysis (TP) is a rare disease with rapid progression and potential fatal evolution. Immediately after the diagnosis, removal of all ticks from the body of the patient is mandatory. The present study reports for the first time a human case of the disease in Brazil. The patient had loss of muscle strength, decreased reflexes and marked palpebral ptosis. Six hours after removal of the last tick, the ptosis improved and on the following day, the patient had near total regression of the symptoms. This report emphasizes the possible presence of similar cases that should be promptly diagnosed and quickly treated. A new induction pattern for TP in humans associated with immature stages of ticks is also presented.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Tick paralysis; Immature ticks; Ixodidae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400017

Combination of heterologous fibrin sealant and bioengineered human embryonic stem cells to improve regeneration following autogenous sciatic nerve grafting repair
Provedor de dados: 80
Autores: Mozafari,Roghayeh; Kyrylenko,Sergiy; Castro,Mateus Vidigal; Ferreira Jr,Rui Seabra; Barraviera,Benedito; Oliveira,Alexandre Leite Rodrigues.
Abstract  Background Peripheral nerve injury is a worldwide clinical problem, and the preferred surgical method for treating it is the end-to-end neurorrhaphy. When it is not possible due to a large nerve gap, autologous nerve grafting is used. However, these surgical techniques result in nerve regeneration at highly variable degrees. It is thus very important to seek complementary techniques to improve motor and sensory recovery. One promising approach could be cell therapy. Transplantation therapy with human embryonic stem cells (hESCs) is appealing because these cells are pluripotent and can differentiate into specialized cell types and have self-renewal ability. Therefore, the main objective of this study was to find conditions under which functional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurorrhaphy; Sciatic nerve; Human embryonic stem cells; Fibrin sealant; FGF2.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100305

A secure and economical system for caging venomous snakes
Provedor de dados: 80
Autores: Powell,R. L.
This paper describes an inexpensive and secure cage system for housing venomous snakes. The cages are easily constructed from commercially available plastic containers and are lightweight and can be stacked, minimizing the area needed to house numerous animals. They allow easy access to the animal and can be adequately disinfected. These cages can be individually locked and also allow for full viewing of the animal.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cage system; Venomous snakes; Security; Lockable; Lightweight.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100008

Assessment of neuropharmacological potential of low molecular weight components extracted from Rhinella schneideri toad poison
Provedor de dados: 80
Autores: Baldo,Mateus Amaral; Cunha,Alexandra Olimpio Siqueira; Godoy,Lívea Dornela; Liberato,José Luiz; Yoneda,Juliana Sakamoto; Fornari-Baldo,Elisa Correa; Ciancaglini,Pietro; Santos,Wagner Ferreira dos; Arantes,Eliane Candiani.
Abstract  Background: Studies on toad poison are relevant since they are considered a good source of toxins that act on different biological systems. Among the molecules found in the toad poison, it can be highlighted the cardiotonic heterosides, which have a known mechanism that inhibit Na+/K+-ATPase enzyme. However, these poisons have many other molecules that may have important biological actions. Therefore, this work evaluated the action of the low molecular weight components from Rhinella schneideri toad poison on Na+/K+-ATPase and their anticonvulsive and / or neurotoxic effects, in order to detect molecules with actions of biotechnological interest.  Methods: Rhinella schneideri toad (male and female) poison was collected by pressuring their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhinella schneideri; Toad poison; Bufadienolides; Seizures; Neuroprotection.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100309

Mild reproductive effects of the Tityus bahiensis scorpion venom in rats
Provedor de dados: 80
Autores: Dorce,Ana Leticia C; Dorce,Valquiria AC; Nencioni,Ana Leonor A.
BackgroundScorpion envenoming is a public health problem in Brazil, whereTityus serrulatus and T. bahiensisare considered the most dangerous scorpions. They are well adapted to urbanized environments, and there is an increasing probability of human exposure to these venoms, including during pregnancy. Not much is known about the effects of prenatal exposure to the venom, and no information is available to aid in the rational treatment of victims stung during pregnancy. Thus, this study aimed to investigate whether venom from the scorpion T. bahiensis administered once to pregnant female rats at a dose that causes a moderate envenomation may lead to deleterious effects on the reproductive performance of the dams and on the development of their offspring....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venom; Tityus bahiensis; Pregnancy; Reproductive development; Embryofetotoxicity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200322

Synergic effects between ocellatin-F1 and bufotenine on the inhibition of BHK-21 cellular infection by the rabies virus
Provedor de dados: 80
Autores: Cunha Neto,Rene dos Santos; Vigerelli,Hugo; Jared,Carlos; Antoniazzi,Marta Maria; Chaves,Luciana Botelho; Silva,Andréa de Cássia Rodrigues da; Melo,Robson Lopes de; Sciani,Juliana Mozer; Pimenta,Daniel C.
Abstract  Background  Rabies is an incurable neglected zoonosis with worldwide distribution characterized as a lethal progressive acute encephalitis caused by a lyssavirus. Animal venoms and secretions have long been studied as new bioactive molecular sources, presenting a wide spectrum of biological effects, including new antiviral agents. Bufotenine, for instance, is an alkaloid isolated from the skin secretion of the anuran Rhinella jimi that inhibits cellular penetration by the rabies virus. Antimicrobial peptides, such as ocellatin-P1 and ocellatin-F1, are present in the skin secretion of anurans from the genus Leptodactylus and provide chemical defense against predators and microorganisms.  Methods  Skin secretion from captive Leptodactylus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies virus; Ocellatin-F1; Bufotenine Toxin; Leptodactylus.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100352

Putative new heat-stable cytotoxic and enterotoxic factors in culture supernatant of Escherichia coli isolated from drinking water
Provedor de dados: 80
Autores: Ribeiro,DA; Niemann,FS; Gatti,MSV; Lanna,MCS; Tsuji,T; Yano,T.
Enteric infections caused by the ingestion of contaminated water, especially by Escherichia coli, are important to define the virulence properties of these bacteria. Due to frequent infantile diarrhea in the city of Ouro Preto, Minas Gerais state, Brazil, the phenotypic and genotypic diarrheagenic properties of E. coli isolated from drinking water were studied. The culture supernatants of 39 (40%) among a total of 97 E. coli isolates from drinking water were positive by suckling mouse assay and induced cytotoxic effects on Vero cells. The enterotoxic and cytotoxic activities were present in the fraction with less than 10 kDa and were not lost when heated up to 60°C and 100°C for 30 minutes. PCR assays showed that among these 39 Vero cytotoxigenic E. coli,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Drinking water; Cytotoxin; Enterotoxin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100014

An update on the epidemiological situation of spotted fever in Brazil
Provedor de dados: 80
Autores: Oliveira,Stefan Vilges de; Guimarães,Jessica Noronha; Reckziegel,Guilherme Carneiro; Neves,Bidiah Mariano da Costa; Araújo-Vilges,Keline Medeiros de; Fonseca,Lidsy Ximenes; Pinna,Fernanda Voietta; Pereira,Simone Valéria Costa; Caldas,Eduardo Pacheco de; Gazeta,Gilberto Salles; Gurgel-Gonçalves,Rodrigo.
Abstract  Background  Spotted fever is a tick-borne rickettsial disease. In Brazil, its notification to the Ministry of Health is compulsory. Since 2007, cases of spotted fever have been integrated to the Notifiable Diseases Information System, and epidemiological analyzes are part of the routines on surveillance programs.  Methods  This descriptive study updates epidemiological information on cases of spotted fever registered in Brazil between 2007 and 2015.  Results  In Brazil, 17,117 suspected cases of the disease were reported and 1,245 were confirmed in 12 states, mainly in São Paulo (550, 44.2 %) and Santa Catarina (276, 22.2 %). No geographic information was registered for 132 cases (10.6 %). Most of the infected people were men (70.9 %), mainly in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rickettsial diseases; Epidemiology; Information system; Tick-borne disease.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100315

Risks of vaccination: a review
Provedor de dados: 80
Autores: Rashid,A; Rasheed,K; Asim,M; Hussain,A.
To be granted a product license, a vaccine must present adequate quality, safety and efficacy. Studies on these criteria often utilize target species in a laboratory setting. Vaccines that had been proven effective during laboratory analysis may not present the same features when sold on a large scale, after encountering field conditions, and furthermore, can even produce complications. Measures are already in place to detect adverse reactions as reported by veterinarians to manufacturers, so that vaccines under suspicion of failing can be identified and investigated. The present review article describes the main problems, specifically adverse reactions and lack of efficacy, that have been encountered following release of vaccines for general use.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vaccine; Quality; Safety; Efficacy; Adverse reactions.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100003

Mycobacterium genavense infection in two species of captive snakes
Provedor de dados: 80
Autores: Ullmann,Leila Sabrina; Dias,Ramiro das Neves; Cagnini,Didier Quevedo; Yamatogi,Ricardo Seiti; Oliveira,Jose Paes; Nemer,Viviane; Teixeira,Rodrigo Hidalgo Friciello; Biondo,Alexander Welker; Araújo Jr.,João Pessoa.
Abstract  Background: Mycobacterium is an important zoonotic agent with companion, livestock and wildlife animals reportedly playing a role as reservoirs. Although its association with reptiles has been described, the disease cycle remains to be fully established, particularly in snakes. Accordingly, this study aimed to report the occurrence of mycobacteriosis with clinical pneumonia in one exotic python snake (Python molurus) and one native green snake (Philodryas olfersii) from the Sorocaba Zoo, São Paulo state, Brazil.  Methods: Diagnosis was based on necropsy, histopathological examination, Ziehl-Neelsen stain and immunohistochemistry.  Results: Using a nested PCR followed by DNA sequencing and bioinformatics analysis, the causative Mycobacterium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Captive snakes; Mycobacterium; Public health.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100320

Virulence factors and antibiotic resistance of Helicobacter pylori isolated from raw milk and unpasteurized dairy products in Iran
Provedor de dados: 80
Autores: Mousavi,Soolmaz; Dehkordi,Farhad Safarpoor; Rahimi,and Ebrahim.
Background Despite the high importance of Helicobacter pylori, the origin and transmission of this bacterium has not been clearly determined. According to controversial theories and results of previous studies, animal source foods – especially milk – play an important role in the transmission of H. pylori to humans. The aim of the present study was to determine the distribution of vacA, cagA,iceA and oipA virulence factors inH. pylori strains isolated from milk and dairy products and study their antimicrobial resistance properties.Methods A total of 520 raw milk and 400 traditional dairy product samples were cultured and tested. Those that were H. pylori-positive were analyzed for the presence of vacA,cagA, iceA and oipAvirulence factors. Antimicrobial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Virulence factors; Antibiotic resistance properties; Milk; Dairy products; Iran.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200341

Impact of leishmaniasis on public health
Provedor de dados: 80
Autores: Camargo,L. B; Langoni,H.
Leishmaniasis is a parasitic zoonosis caused by protozoans of the genus Leishmania transmitted by insects known as phlebotomines, which are found in wild or urban environments. It affects domestic and wild animals and transmission to man happens by accident. The disease occurs in tropical and sub-tropical areas, mainly in Asia, Europe, Africa, and the Americas. There are two forms that affect man: American cutaneous leishmaniasis (ACL) and American visceral leishmaniasis (AVL). The latter is caused by three species of Leishmania: Leishmania (Leishmania) donovani, Leishmania (Leishmania) infantum, and Leishmania (Leishmania) chagasi, which are grouped in the Leishmania (Leishmania) donovani complex. Wild reservoir hosts of L. chagasi known so far are foxes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Cutaneous leishmaniasis; Visceral leishmaniasis; Leishmania.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400002

Poison as cure: a clinical review of botulinum toxin as an invaluable drug
Provedor de dados: 80
Autores: Bali,J.; Thakur,R.
Botulinum toxin is the most potent toxin known. It is readily absorbed from mucosal surfaces. If dispersed as an aerosol or mixed in the food or water it can lead to a large outbreak of botulism. The disease presents as a symmetric descending paralysis in an afebrile patient. Cranial nerve involvement with diplopia, dysarthria, dysphonia, dysphagia and respiratory paralysis is seen after a variable incubation period. The treatment is mainly supportive. The source of the toxin is Clostridium botulinum, an anaerobic gram-positive spore-forming organism. Some other species of Clostridium like C. butyricum and C. baratii also produce the toxin. The toxin is heat labile and can be inactivated by heating at 100°C for 10 minutes. The toxin acts at the peripheral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Botulinum toxin; Mechanism of action; Botulism; Therapeutics.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400003

The fire consumed... a treasure!
Provedor de dados: 80
Autores: Sant'Anna,OA.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300004

Effect of prostaglandins on the production of H2O2 and cytokines that modulate the fungicidal activity of human monocytes against Paracoccidioides brasiliensis
Provedor de dados: 80
Autores: Bordon,A. P.
Tipo: Info:eu-repo/semantics/other
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100013

Neurological complications and death in children with dengue virus infection: report of two cases
Provedor de dados: 80
Autores: Osnaya-Romero,Neydi; Perez-Guille,Maria-Gabriela; Andrade-García,Sandra; Gonzalez-Vargas,Erika; Borgaro-Payro,Rebeca; Villagomez-Martinez,Sandra; Ortega-Maldonado,José de Jesús; Arredondo-García,Jose Luis.
Abstract  Background Dengue virus infection can have different complications; the best known is hemorrhagic dengue fever. However, other effects such as neurological disorders may endanger the lives of patients. Dengue neurological manifestations can be confused with encephalitis symptoms and can lead to cerebral edema and death. Therefore, we consider important in the endemic areas to take into account the diagnosis of dengue encephalitis in patients with neurological disorders, and to request the determination of serology in cerebrospinal fluid for the NS1 antigen test.  Case presentation We present the cases of two patients from the state of Morelos, Mexico, with 17 and 14 years of age. Both cases presented a rapid evolution characterized by fever,...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Dengue; Dengue virus; Neurological disorders; Death; Children.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100505

Fatal bothropic snakebite in a horse: a case report
Provedor de dados: 80
Autores: Silva,NS; Silveira,JAS; Albernaz,TT; Campos,KF; Oliveira,CMC; Freitas,NFQR; Bomjardim,HA; Barbosa,JD.
The present study reports a snakebite in a horse in the state of Pará, Brazil. At initial evaluation the animal was reluctant to walk and had tachycardia, tachypnea, severe lameness, bleeding on the pastern and swelling around the left hind leg. Blood samples from the bleeding sites, took on the first day, showed leukocytosis and neutrophilia, whereas biochemical values of urea and creatinine were significantly increased. The chosen treatment was snake antivenom, fluid therapy, antibiotics, anti-inflammatory agents and diuretic drugs. On the fourth day of therapy, the hematological values were within normal parameters. There was improvement related to the clinical lameness and swelling of the limb. However, a decrease in water intake and oliguria were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snakebites; Horses; Bothrops.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400018

Lack of correlation between rabies virus replication in the brain and antibody isotype profile in genetically modified mice
Provedor de dados: 80
Autores: Consales,C. A.; Pereira,C. A.; Passos,E. C.; Carrieri,M. L.; Galina,N. M. F.; Sant'Anna,O. A.
The relationship among the phenotypes resistance to infection, virus replication in the brain and isotype production was investigated in genetically modified High (H) or Low (L) antibody responder mouse lines. Although they express the same innate susceptibility to rabies infection, these lines differ as to different viral replication rates in the central nervous system and L mice showed a higher permissible state. After intramuscular infection with the Pasteur rabies strain (PV), the H-L interline differences on the earlier stage of virus replication were 1000 and 80 folds on days 5 and 6, respectively. The isotype profile in sera of the experimentally infected mice reflected an interline difference of 25 folds for IgG2a throughout the infection period,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Infection; Isotypes; Nervous system.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300007

Vital network for Brazil: national network of information, discussion and cooperation concerning venomous animals
Provedor de dados: 80
Autores: Brazil,TK; Melgarejo,AR; Werneck de Castro,AJ; Barraviera,B; Souza,CM; Brazil,EV; Cotta,GA; Puorto,G; Pauli,I; Minozzo,JC; Prado-Franceschi,J; Cunha,LER; Lima,MVC; Lira-da-Silva,RM; Bochner,R; Ferreira Júnior,RS; Souza,SP; Queiroz,VS.
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300001

Anticancer potential of nanogold conjugated toxin GNP-NN-32 from Naja naja venom
Provedor de dados: 80
Autores: Attarde,Saurabh S.; Pandit,Sangeeta V.
Abstract  Background: Cancer is the second most common fatal disease in the world, behind cardiovascular disorders in the first place. It accounts for around 0.3 million deaths per year in India due to the lack of proper diagnostic facilities, prevention and treatment. Current therapeutic methods do not provide adequate protection and affect normal cells along with cancerous ones. Thus, there is a need for some alternative therapeutic strategy, preferably from natural products, which have been traditionally used for treatment of various diseases in the country.  Methods: In this study, we have conjugated purified NN-32 toxin from Naja naja venom with gold nanoparticles and its anticancer potential was evaluated against human breast cancer cell lines....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GNP-NN-32; Anticancer; Toxin; Naja naja; Venom; Breast cancer; MCF-7; MDA-MB-231.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100304

Clinical and immunological aspects of envenomations by Bothrops snakes
Provedor de dados: 80
Autores: Luna,KPO; da Silva,MB; Pereira,VRA.
Accidents caused by snakes, especially in tropical and subtropical countries, still constitute a serious public health problem due to the lack of knowledge of health professionals and the precariousness of health systems in the regions where most accidents occur. Snake venoms contain a range of molecules that may provoke local swelling, pain, renal and respiratory insufficiencies. The study of the effects of each molecule on humans can help the development of complementary therapy. Similarly, the knowledge of clinical aspects of envenomations provides a better identification and implementation of appropriate treatment. In addition, to understand Bothrops envenomations and improve the therapeutic strategy, it is necessary to understand and study the role of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snakes; Envenomations; Clinical aspects; Immunology.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200003

A newly described scorpion species, Leiurus abdullahbayrami (Scorpion: Buthidae), and the lethal potency and in vivo effects of its venom
Provedor de dados: 80
Autores: Ozkan,O; Yagmur,EA; Ark,M.
Currently, medically significant scorpion species belong to the Buthidae family and are represented by the genera Androctonus, Buthus, Mesobuthus, Hottentotta, Parabuthus, Tityus, Centruroides, Leiurus. Although Leiurus was originally considered a monotypic genus, four additional species have since been described. Leiurus abdullahbayrami (previously identified as L. quinquestriatus in Turkey) was classified as a new Leiurus species. This is the first report conducted on the lethality and biologic effects of L. abdullahbayrami scorpion venom in mice. In this study, the electrophoretic protein pattern of its venom was also determined. Two protein bands with molecular masses of 4 and 6 kDa were more strongly detected than other protein bands in the venom...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpions; Leiurus spp.; Scorpion venom; Lethality; In vivo effects.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400008

A Bayesian approach to estimate the accuracy of "in-house" ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle
Provedor de dados: 80
Autores: Nogueira,AHC; Silva,C; Gomes,DE; Rosa,ACG; Luvizotto,MCR; Cardoso,TC.
Rabies is a vaccine-preventable disease that causes acute encephalitis in mammals, and it is still a significant public health problem in numerous countries. Infected dogs represent the main vectors involved in human rabies. Additionally, cattle rearing close to geographic areas where vampire bats are found presents an important connection with rural epidemiology. We applied two "in-house" enzyme-linked immunosorbent assay (ELISA) methodologies, considered alternatives to measure antibodies from vaccinated dogs and cattle, without employing the gold standard approach. The ELISA assays were performed on individual serum samples taken from domestic adult dogs and cows compulsory vaccinated against rabies (147 urban dogs and 64 cows; n = 211). The sandwich...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies antibody; Bayesian analysis; ELISA assay.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100012

Bacterial biofilms with emphasis on coagulase-negative staphylococci
Provedor de dados: 80
Autores: Oliveira,A; Cunha,MLRS.
In addition to their capacity to attach to surfaces, various groups of microorganisms also produce an extracellular polymeric substance known as "slime". This slime forms a thin layer around cells known as biofilm. Thus, biofilm structure comprises bacterial cells and an extracellular polymeric substance. It also presents a defined architecture, providing the microorganisms with an excellent protective environment and favoring the exchange of genetic material between cells as well as intercellular communication. The ability to produce biofilm is observed in a large group of bacteria, including coagulase-negative staphylococci (CNS) which are the predominant microorganisms of normal skin flora and have been implicated as the causative agents of hospital...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coagulase-negative staphylococci; Biofilm; Slime; Infection; Microbiology.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400003

Diagnosis of paracoccidioidomycosis in patients attended in routine services of a university hospital
Provedor de dados: 80
Autores: Moreto,TC; Mendes,RP.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300021

Animal toxins: state of the art - perspectives in health and biotechnology
Provedor de dados: 80
Autores: Lima,Maria Elena de; Pimenta,Adriano Monteiro de Castro; Martin-Eauclaire,Marie France; Zingali,Russolina Benedeta; Rochat,Hervé.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300021

Lipid profile of HIV-1 infected patients under highly active antiretroviral therapy
Provedor de dados: 80
Autores: Pontes,L. C. R.
Tipo: Info:eu-repo/semantics/other
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300011

Diagnosis of arboviruses using indirect sandwich IgG ELISA in horses from the Brazilian Amazon
Provedor de dados: 80
Autores: Casseb,Alexandre do Rosário; Nunes,Márcio Roberto Teixeira; Rodrigues,Sueli Guerreiro; Rosa,Elizabeth Salbé Travassos da; Casseb,Livia Medeiros Neves; Casseb,Samir Manssor Moraes; Silva,Sandro Patroca da; Rodrigues,Érika Dayane Leal; Vasconcelos,Pedro Fernando da Costa.
Background The Amazon as a whole is the largest reservoir of arboviruses worldwide, while the Brazilian Amazon hosts the largest variety of arboviruses isolated to date. In this study, the results of an indirect sandwich IgG ELISA, standardized for 19 arbovirustypes circulating among horses in Brazilian Amazon, were compared to results of the hemagglutination inhibition test. A screening test assessed the conditional probability distribution and a Pearson linear correlation test determined the correlation strength among the absorbance values recorded for viruses from the same family.Findings Sensitivity varied between 40.85 and 100%; the specificity was low and ranged from 39.71 to 67.0%; and the accuracy varied between 41 and 65.2%. The test developed in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Horses; Arboviruses; ELISA.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200601

Use of computed tomography in the detection of adrenal gland involvement in tuberculosis patients
Provedor de dados: 80
Autores: Yamashita,S.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100021

A biodistribution study of Hemiscorpius lepturus scorpion venom and available polyclonal antivenom in rats
Provedor de dados: 80
Autores: Seyedian,R; Jalali,A; Babaee,MH; Pipelzadeh,MH; Rezaee,S.
The purpose of the present study was to investigate the biodistribution profile of the venom of Hemiscorpius lepturus, the most dangerous scorpion in Iran. Blood and tissue samples were taken at various predetermined intervals during a 400-minute period for the venom and a 360-minute period for the antivenom in rats. The radio-iodination was carried out using the chloramine-T method. The results showed that the descending order of venom uptake was skin, kidneys and intestine, respectively. The descending order of polyclonal antivenom uptake was kidneys, intestine, heart and lungs. The calculated pharmacokinetic parameters of the venom were Telimination half-life = 521.5 ± 12.6 minutes; Vd/F (apparent volume of distribution) = 14.9 ± 3.3 mL; clearance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemiscorpius lepturus scorpion venom; Polyvalent antivenom; Pharmacokinetic parameters; Tissue distribution.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400005

Scientific communication: how to make it effectively?
Provedor de dados: 80
Autores: Goldenberg,Saul.
Tipo: Info:eu-repo/semantics/article
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100001

Antivenom action on renal effects induced by Thalassophryne nattereri venom
Provedor de dados: 80
Autores: Martins,AMC; Barbosa,PSF; Sousa,DF; Alves,CD; Menezes,DB; Lima,C; Lopes-Ferreira,M; Fonteles,MC; Monteiro,HSA.
Thalassophryne nattereri (niquim) is a venomous fish responsible for numerous accidents involving fishermen in northern and northeastern Brazil. The aim of the present investigation was to evaluate the action of antivenom on renal effects caused by Thalassophryne nattereri venom. Isolated kidneys of Wistar rats were perfused with a previously dialyzed Krebs-Henseleit solution containing 6 g% bovine serum albumin. The antivenom action was studied through perfusion pressure (PP), renal vascular resistance (RVR), urinary flow (UF) and glomerular filtration rate (GFR). The niquim venom (1 µg/mL), the antivenom alone (1 µg/mL) or the venom incubated with antivenom were added to the system 30 minutes after the beginning of each perfusion. Previous works have...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thalassophryne nattereri; Antivenom; Kidney.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100011

Novel role of antiplatelet agents (aspirin plus clopidogrel) in an incoagulable blood of a victim of russell's viper snakebite
Provedor de dados: 80
Autores: Bawaskar,H. S.
Snake antivenom is a specific antidote to the venom action, neutralizing the circulating venom. However, it fails to neutralize the venom fixed to target organs such as platelets, renal tubules, etc. Russell's viper venom initiates rapid coagulation in a victim by activating blood platelets, factors V, X, and anticoagulant cofactors. Activation of thrombin, resulting in formation of micro-thrombi, fibrinolysis, and a vicious cascade, sets in. Inhibition of activated platelets by aspirin (cyclooxygenase inhibitor) and clopidogrel (ADP receptor inhibitor) helps to break this vicious circle induced by Russell's venom and may initiate the natural physiological clotting mechanism. They can be utilized as an adjuvant treatment.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Russell's viper; Coagulation; Fibrinolysis; Aspirin; Clopidogrel.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100011

Antibody and cytokine serum levels in patients submitted to anti-rabies prophylactic treatment with serum vaccination
Provedor de dados: 80
Autores: Ayres,J.
Tipo: Info:eu-repo/semantics/article
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200010

Interval between first dose and booster affected antibody production in cattle vaccinated against rabies
Provedor de dados: 80
Autores: Albas,A.; Fontolan,O. L.; Pardo,P. E.; Bremer Neto,H.; Sartori,A.
In this study, we compared the levels of neutralizing antibodies induced by inactivated rabies vaccine in cattle by using three alternative immunization procedures. Forty-five bovines (breed nelore) were then organized in three groups (A, B and C, with 15 animals/group). Group A received only one vaccine dose at day zero and Group B received the first dose at day zero and then another dose at day 30 (early booster). Group C was also immunized with two doses; however, the booster was postponed until day 180 after the first dose (delayed booster). Blood samples were withdrawn at days zero (before the first dose) and 30, 210, 390, and 540 after the beginning of immunization and the antibody titers were evaluated by mouse neutralization test. The protocol used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Vaccine; Cattle; Antibody; Neutralization.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300010

A checklist of the scorpions of Ecuador (Arachnida: Scorpiones), with notes on the distribution and medical significance of some species
Provedor de dados: 80
Autores: Brito,Gabriel; Borges,Adolfo.
Ecuador harbors one of the most diverse Neotropical scorpion faunas, hereby updated to 47 species contained within eight genera and five families, which inhabits the “Costa” (n= 17), “Sierra” (n= 34), “Oriente” (n= 16) and “Insular” (n= 2) biogeographical regions, corresponding to the western coastal, Andean, Amazonian, and the Galápagos archipelago regions, respectively. The genusTityus Koch, in the family Buthidae, responsible for severe/fatal accidents elsewhere in northern South America and the Amazonia, is represented in Ecuador by 16 species, including T. asthenes, which has caused fatalities in Colombia and Panama, and now in the Ecuadorian provinces of Morona Santiago and Sucumbíos. Underestimation of the medical significance of scorpion envenoming...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpions; Ecuador; Ananteris; Brachistosternus; Chactas; Centruroides; Hadruroides; Scorpionism; Teuthraustes; Tityus; Troglotayosicus.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100210

BthTX-I from Bothrops jararacussu induces apoptosis in human breast cancer cell lines and decreases cancer stem cell subpopulation
Provedor de dados: 80
Autores: Bezerra,Patrícia Heloise Alves; Ferreira,Isadora Marques; Franceschi,Beatriz Tinoco; Bianchini,Francine; Ambrósio,Luciana; Cintra,Adélia Cristina O.; Sampaio,Suely Vilela; Castro,Fabíola Attié de; Torqueti,Maria Regina.
ABSTRACT  Background: Breast cancer is the neoplasm with both the highest incidence and mortality rate among women worldwide. Given the known snake venom cytotoxicity towards several tumor types, we evaluated the effects of BthTX-I from Bothrops jararacussu on MCF7, SKBR3, and MDAMB231 breast cancer cell lines.  Methods:  BthTX-I cytotoxicity was determined via MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide assay. Cell death was measured by a hypotonic fluorescent solution method, annexin-V-FITC/propidium iodide staining and by apoptotic/autophagic protein expression. Cancer stem cells (CSCs) were quantified by flow cytometry using anti-CD24-FITC and anti-CD44-APC antibodies and propidium iodide.  Results:  BthTX-I at 102 µg/mL induced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Bothropstoxin; Breast cancer; Cancer stem cells.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100315

Anti-Trimeresurus albolabris venom IgY antibodies: preparation, purification and neutralization efficacy
Provedor de dados: 80
Autores: Duan,Hai-long; He,Qi-yi; Zhou,Bin; Wang,Wen-wen; Li,Bo; Zhang,Ying-zheng; Deng,Qiu-ping; Zhang,Ying-feng; Yu,Xiao-dong.
Abstract  Background  Snakebite incidence in southwestern China is mainly attributed to one of the several venomous snakes found in the country, the white-lipped green pit viper Trimeresurus albolabris. Since antivenom produced from horses may cause numerous clinical side effects, the present study was conducted aiming to develop an alternative antivenom antibody (immunoglobulin Y - IgY) from leghorn chickens.  Methods  IgY in egg yolk from white leghorn chicken previously injected with T. albolabris venom was extracted by water, precipitated by ammonium sulfate and purified by affinity chromatographic system. IgY was identified by SDS-PAGE, ELISA and Western blot, and its neutralizing assay was conducted on mice.  Results  Chickens injected multiple times...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IgY; Egg yolk; Snake venom; Trimeresurus albolabris; LD50.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100316

Serum levels of cytokines in different stages of the HIV-1 infection and their correlation with beta2-microglobulin serum level, hemoglobin rate, T CD4+ and T CD8+ lymphocytes count
Provedor de dados: 80
Autores: Antunes,M. C.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100021

Association among H. pylori virulence markers dupA, cagA and vacA in Brazilian patients
Provedor de dados: 80
Autores: Pereira,Weendelly Nayara; Ferraz,Mariane Avante; Zabaglia,Luanna Munhoz; Labio,Roger William de; Orcini,Wilson Aparecido; Ximenez,João Paulo Bianchi; Caleman Neto,Agostinho; Payão,Spencer Luiz Marques; Rasmussen,Lucas Trevizani.
Background:Only a few Helicobacter pylori-infected individuals develop severe gastric diseases and virulence factors of H. pyloriappear to be involved in such clinical outcomes. Duodenal ulcer promoting gene A (dupA) is a novel virulence factor ofHelicobacter pylori that is associated with duodenal ulcer development and reduced risk for gastric carcinoma in some populations. The aims of the present study were to determine the presence ofdupA gene and evaluate the association amongdupA and other virulence factors includingcagA and vacA in Brazilian patients. Gastric biopsies were obtained from 205 dyspeptic patients (100 children and 105 adults). DNA was extracted and analyzed for the presence of H. pylori and its virulence factors using the polymerase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; DupA; CagA; VacA; Gastroduodenal diseases; PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200319

Histological characterization of Sticholecitha serpentis Prudhoe, 1949 (digenea, bieriidae, sticholecithinae), parasite of Bothrops moojeni Hoge, 1966 (serpentes, viperidae)
Provedor de dados: 80
Autores: Silva,TB.; Rossellini,M.; Dal Pai Silva,M.; Silva,RJ.
The aim of this study was to evaluate the morphology of the species Sticholecitha serpentis Prudhoe, 1949 by means of histological procedures. Helminths were previously fixed in Railliet &amp;amp; Henry solution, uncompressed and were included in 2-hydroxyethyl-methacrylate. Longitudinal and transverse serial sections with a 4-mum thickness were performed in a microtome (Leica RM 2165), stained with haematoxylin-eosin and then analyzed in a computerized image analysis system (Qwin Lite 2.5, Leica). Structures of systematic value, such as oral sucker, acetabulum, prepharynx, pharynx, esophagus, intestinal caeca, vitelline glands, ovary, uterus, cirrus pouch and testicles were described. Structures that were poorly visible in total preparations were also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sticholecitha serpentis; Digenea; Bothrops moojeni; Snakes; Brazil.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400010

Hepatotoxicity and oxidative stress induced byNaja haje crude venom
Provedor de dados: 80
Autores: Al-Quraishy,Saleh; Dkhil,Mahamed A; Moneim,Ahmed Esmat Abdel.
Background Snake venoms are synthesized and stored in venom glands. Most venoms are complex mixtures of several proteins, peptides, enzymes, toxins and non-protein components. In the present study, we investigated the oxidative stress and apoptosis in rat liver cells provoked by Naja haje crude injection (LD50) after four hours.Methods Wistar rats were randomly divided into two groups, the control group was intraperitoneally injected with saline solution while LD50-dose envenomed group was intraperitoneally injected with venom at a dose of 0.025 μg/kg of body weight. Animals were killed four hours after the injection. Lipid peroxidation, nitric oxide and glutathione levels were measured as oxidative markers in serum and liver homogenate. In addition, liver...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Naja haje venom; Hepatotoxicity; Oxidative stress; Apoptosis; Rats.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200314

Dose and time dependence of box jellyfish antivenom
Provedor de dados: 80
Autores: Andreosso,Athena; Smout,Michael J; Seymour,Jamie E.
Background The effectiveness of the currently available box jellyfish (Chironex fleckeri) antivenom has been subject of debate for many years. To assess whether the box jellyfish antivenom has the ability to attenuate venom-induced damage at cellular level, the present study analyzed the dose and time dependence of the antivenom in a cell-based assay.Methods Different doses of antivenom were added to venom and subsequently administered to cells and the cell index was measured using xCelligence Technology (ACEA Biosciences). Similarly, antivenom and venom were incubated over different time periods and cell survival measured as stated above. For both experiments, the cell index was plotted as a measure of cell survival against the dose or incubation time and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chironex fleckeri; Antivenom; Venom; Dose–response curve.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200335

Interactions with extracellular matrix and epithelial cellular cytoskeletal involvement during Paracoccidioides brasiliensis adhesion and invasion
Provedor de dados: 80
Autores: Hanna,S. A.
Tipo: Info:eu-repo/semantics/article
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200011

Peptidomic analysis of the venom of the solitary bee Xylocopa appendiculata circumvolans
Provedor de dados: 80
Autores: Kazuma,Kohei; Ando,Kenji; Nihei,Ken-ichi; Wang,Xiaoyu; Rangel,Marisa; Franzolin,Marcia Regina; Mori-Yasumoto,Kanami; Sekita,Setsuko; Kadowaki,Makoto; Satake,Motoyoshi; Konno,Katsuhiro.
Abstract  Background: Among the hymenopteran insect venoms, those from social wasps and bees - such as honeybee, hornets and paper wasps - have been well documented. Their venoms are composed of a number of peptides and proteins and used for defending their nests and themselves from predators. In contrast, the venoms of solitary wasps and bees have not been the object of further research. In case of solitary bees, only major peptide components in a few venoms have been addressed. Therefore, the aim of the present study was to explore the peptide component profile of the venom from the solitary bee Xylocopa appendiculata circumvolans by peptidomic analysis with using LC-MS.  Methods: A reverse-phase HPLC connected to ESI-OrbiTrap MS was used for LC-MS....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peptidomic analysis; LC-ESI-MS; Solitary bee; Venom; Linear cationic α-helical peptide.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100316

Different nutritional-state indicators of HIV-positive individuals undergoing antiretroviral therapy
Provedor de dados: 80
Autores: Geraix,J.; Carvalhaes,M. A. B. L.; Pereira,P. C. M.
This study aimed at learning about the nutritional profile of HIV-positive individuals undergoing antiretroviral therapy and at comparing the performance of nutritional-state indicators. A transversal study was performed on 94 patients attending the Tropical Diseases Outpatient Hospital of Botucatu Medical School (FMB) - UNESP. The body mass index (BMI) and the classification by Papini-Berto (PB) were used to evaluate nutritional state, aiming at detecting malnutrition and obesity. The waist-to-hips ratio (W/HR) and waist circumference (WC) were adopted for identification of abdominal obesity and lipodystrophy. According to BMI, most of the individuals were eutrophic, followed by 30.9% overweight and 6.4% malnourished. By using the PB classification, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; AIDS; Antiretroviral therapy; Nutritional indicators.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200011

Anticancer properties of phospholipase A2 fromDaboia siamensis venom on human skin melanoma cells
Provedor de dados: 80
Autores: Khunsap,Suchitra; Khow,Orawan; Buranapraditkun,Supranee; Suntrarachun,Sunutcha; Puthong,Songchan; Boonchang,and Supatsorn.
Abstract  Background  Phospholipase A2 (PLA2) is a major component of theDaboia siamensis venom, which is able to hydrolyse the membrane of various cells. For this reason, the activity of PLA2was investigated regarding its pharmaceutical properties. This study was conducted to explore the pharmacological properties of a PLA2from Daboia siamensis (dssPLA2) venom on human skin melanoma cell line (SK-MEL-28).  Methods  dssPLA2 was isolated by ion exchange and gel filtration columns. Various concentrations of dssPLA2were investigated for cytotoxic activity and inhibition of migration on SK-MEL-28 cells. Cell death analysis, mRNA expression levels of Notch I-III and BRAF V600E genes were also determined.  Results  dssPLA2 exhibited cytotoxicity on SK-MEL-28 for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phospholipase A2; Daboia siamensis venom; Skin melanoma; Anticancer.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100305

Envenomation by scorpion in dog: case report
Provedor de dados: 80
Autores: Cardoso,M. J. L.; Sakate,M.; Ciampolini,P.; Moutinho,F. Q.; Cherubini,A. L.
A case admitted at the Small Animals Clinics Service, Veterinary Hospital, FMVZ - UNESP, in May, 1999 is described. A Brazilian Terrier dog, 3 years and 3 months old, weighing 1.7 kg was brought after 2 hours and a half from contact with a scorpion (Tityus bahiensis). The dog showed vocalization inserted with drowsiness. Physical examination showed slightly hyperemic mucous, pain, agressiveness, tachypnea, tachycardia, and discrete erythema on the right forelimb palmar face. An anesthetic block was performed around the stung area using 2% lidocaine hydrochloride without vasoconstrictor (10mL). The animal was asymptomatic, after 24 h treatment.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Scorpion; Dog; Envenomation; Poisoning; Tityus bahiensis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000100008

Rhodococcus equi experimental infection: macrophage activation in drug-immunosuppressed mice
Provedor de dados: 80
Autores: Diniz,R.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100012

Biochemical changes and manifestations of envenomation produced by Odonthobuthus doriae venom in rabbits
Provedor de dados: 80
Autores: Mirakabadi,A. Z.; Jalali,A.; Jahromi,A. E.; Vatanpur,H.; Akbary,A.
Many toxins from scorpion venoms cause neurotransmitters release by activating the autonomic system. The aim of the present work was to determine osmotic fragility of red blood cells (RBCs) and serum biochemical changes produced by the venom of Odonthobuthus doriae (O. doriae), a dangerous species of scorpion in Iran. For this study we selected 2 groups, each one containing 10 New Zealand white rabbits weighing 2 ± 0.2 kg. In vivo and in vitro osmotic fragilities as well as packed cell volume (PCV) were determined. Serum was separated and used for determination of glucose, blood urea nitrogen (BUN), creatinine, uric acid (UA), triglycerides, cholesterol, aspartate aminotransferase (AST, EC 2.6.1.1), and alanine aminotransferase (ALT, EC 2.6.1.2). Results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venom; Odonthobuthus doriae; Biochemical changes; Manifestation of envenomation; Osmotic fragility of RBCs.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100006

New record of Haplometroides intercaecalis (Digenea, Plagiorchiidae) infecting a Brazilian snake
Provedor de dados: 80
Autores: Silva,R. J.; Béda,A. F.; Ferreira,V. L.
Phalotris matogrossensis (Serpentes, Colubridae) was reported as a new host for Haplometroides intercaecalis (Digenea, Plagiorchiidae). The host snake was obtained from the municipality of Anastácio, state of Mato Grosso do Sul, Brazil. One specimen of H. intercaecalis was recovered from the esophagus of the host and identified by the intercecal position of the vitellaria in the pre-acetabular region. This paper describes the second report of the occurrence of this trematode in fossorial snakes of the genus Phalotris in the state of Mato Grosso do Sul, Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Digenea; Haplometroides intercaecalis; Plagiorchiidae; Phalotris matogrossensis; Colubridae; New host.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100013

Characterization of Bothrops jararaca snake venom effects on rat vas deferens
Provedor de dados: 80
Autores: Sciani,J. M.
Tipo: Info:eu-repo/semantics/other
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400017

ABO blood groups and Helicobacter pylori cagA infection: evidence of an association
Provedor de dados: 80
Autores: Mattos,DE; Cintra,JR; Mattos,CC Brandão de; Nakashima,F; Silva,RCMA; Moreira,HW; de Mattos,LC.
Diseases resulting from Helicobacter pylori infection appear to be dependent on a host of genetic traits and virulence factors possessed by this microorganism. This paper aimed to investigate the association between the ABO histo-blood groups and H. pylori cagA infections. Genomic DNA samples (n = 110) of gastric biopsies obtained from patients with endoscopic diagnosis of peptic ulcers (n = 25) and chronic active gastritis (n = 85) were analyzed by PCR using specific primers for the cagA gene. Of the samples, 66.4% (n = 73) tested positive and 33.6% (n = 37) negative for the gene. The cagA strain was predominant in peptic ulcers (n = 21; 84.0%) compared with chronic active gastritis (n = 52; 61.2%) (p = 0.05; OR 3.332; 95% CI: 1.050-10.576). Additionally,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ABO blood groups; H. pylori infection; CagA strain; Chronic active gastritis; Peptic ulcers.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100009

Biological characterization of compounds from Rhinella schneideri poison that act on the complement system
Provedor de dados: 80
Autores: Anjolette,Fernando A. P.; Leite,Flávia P.; Bordon,Karla C. F.; Azzolini,Ana Elisa C. S; Pereira,Juliana C.; Pereira-Crott,Luciana S.; Arantes,Eliane C.
Background The skin secretions of toads of the family Bufonidae contain biogenic amines, alkaloids, steroids (bufotoxins), bufodienolides (bufogenin), peptides and proteins. The poison of Rhinella schneideri, formerly classified as Bufo paracnemis, presents components that act on different biological systems, including the complement system. The aim of this study was to isolate and examine the activity ofRhinella schneideri poison (RsP) components on the complement system.Methods The components active on the complement system were purified in three chromatographic steps, using a combination of cation-exchange, anion-exchange and gel filtration chromatography. The resulting fractions were analyzed by SDS-PAGE and screened for their activity in the hemolytic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Complement system; Hemolytic activity; Neutrophil chemotaxis; Rhinella schneideri; Terminal complement complex (SC5b-9); Toad poison.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100334

Canine trypanosomiasis: etiology of infection and implications for public health
Provedor de dados: 80
Autores: Eloy,LJ; Lucheis,SB.
Canine trypanosomiasis, caused by protozoans of the genus Trypanosoma, is divided into two primary types: the American form (Chagas disease), due to Trypanosoma cruzi infection, and the African form (sleeping sickness or surra), provoked by Trypanosomaevansi. This disease was originally enzootic and affected only wild animals, including mammals and birds, which served as reservoirs. Later, it spread to domestic animals such as horses, cattle and dogs. The disease became a zoonosis when contact between rural inhabitants and natural Trypanosoma foci occurred, due to ecological imbalances and increasing migration. Dogs are significantly involved in this context, because they are the main domestic animals and participate in the transmission and maintenance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canine trypanosomiasis; Trypanosoma cruzi; Trypanosoma evansi; Chagas disease; Surra.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400002

Anti-inflammatory activity of the apolar extract from the seaweed Galaxaura marginata (Rhodophyta, Nemaliales)
Provedor de dados: 80
Autores: Rozas,E.; Freitas,J. C.
The red seaweed Galaxaura marginata (Ellis &amp;amp; Solander) Lamouroux, well known by the antibacterial activity of its polar extract and the cytotoxic activity of its oxygenated desmosterol, showed anti-inflammatory action in its apolar fraction. Topical anti-inflammatory activity was observed in samples collected at São Sebastião channel, northern littoral of São Paulo State, Brazil. The apolar extract and its fractions obtained through Thin-Layer Chromatography (TLC) reduced the topical inflammation produced by croton oil in mouse ear. Such data indicated that the apolar extract from the marine red alga G. marginata displayed anti-inflammatory activity (since 1mg/ear extract reduced 95±0.5% inflammation), which could be the result of the synergic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Seaweed; Galaxaura marginata; Inflammation; Croton oil; Edema; Anti-inflammatory agents.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200011

Application of the mammalian glyceraldehyde-3-phosphate dehydrogenase gene for sample quality control in multiplex PCR for diagnosis of leishmaniasis
Provedor de dados: 80
Autores: Gonçalves,SC; Régis-da-Silva,CG; Brito,MEFC; Brandão-Filho,SP; Paiva-Cavalcanti,M.
Leishmaniasis is a neglected disease endemic in five continents. It is a severe disease that may lead to death, and its early detection is important to avoid severe damage to affected individuals. Molecular methods to detect Leishmania are considered alternatives to overcome the limitations presented by conventional methods. The aim of this study was to develop multiplex PCR systems able to detect small amounts of target DNA of Leishmania infantum and Leishmania braziliensis, and the gene coding for glyceraldehyde-3-phosphate dehydrogenase (G3PD) in mammals, enabling quality evaluation of the sample simultaneously with detection of the specific target. The systems created for G3PD recognition were combined with detection systems for L. infantum and L....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Diagnosis; Glyceraldehyde-3-phosphate dehydrogenase; Quality control.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200009

Hemorrhagic stroke in children caused by Bothrops marajoensis envenoming: a case report
Provedor de dados: 80
Autores: Pardal,Pedro Pereira de Oliveira; Pinheiro,Augusto Cezar Jennings da Silva; Silva,Cristiane Tarcis Cunha; Santosand,Paulo Roberto Silva Garcez; Gadelha,Maria Apolônia da Costa.
Abstract According to the World Health Organization, snakebites are considered neglected diseases. Bothrops, the genus most frequently implicated in envenomations in Brazil, includes the species B. marajoensis Hoge, 1966, part of the complex B. atrox, which is found in the savannas of Marajó Island, Pará state, Brazil, a region that presents scarce epidemiological data. This work reports the first case of hemorrhagic stroke in a child, attributed to delayed medical care after snakebite envenoming by Bothrops marajoensis in Anajás city, Marajó Island, Pará, Brazil, which led to permanent hemiplegia as a sequela.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Hemorrhagic stroke Sequel; Bothrops marajoensis; Marajó Island.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100414

Tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta (TGF-beta) in patients with active paracoccidioidomycosis
Provedor de dados: 80
Autores: Macedo,A. M. R. B.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100016

Trends in multidrug-resistant tuberculosis
Provedor de dados: 80
Autores: Dias-Baptista,I. M. F.; Usó,S. M. R. S.; Marcondes-Machado,J.
Multidrug-resistant tuberculosis (MDR-TB) is an increasing global problem. The extent and burden of MDR-TB varies significantly from country to country and region to region. Globally, about three per cent of all newly diagnosed patients have MDR-TB and the proportion is higher in patients who had previously received anti-tuberculosis (anti-TB) treatment reflecting the failure of programs designed to ensure complete cure of patients with tuberculosis. The management of MDR-TB is a challenge that should be undertaken by experienced clinicians at centers equipped with reliable laboratory services and implementation of DOTS-Plus strategy.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; MDR-TB; XDR-TB; DOTS; DOTS-Plus.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200003

Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strains
Provedor de dados: 80
Autores: Fortaleza,CMCB; Bacchi,CE; Oliveira,DE; Ramos,MC.
Pseudomonas aeruginosa infections cause significant mortality and morbidity in health care settings. Strategies to prevent and control the emergence and spread of P. aeruginosa within hospitals involve implementation of barrier methods and antimicrobial stewardship programs. However, there is still much debate over which of these measures holds the utmost importance. Molecular strain typing may help elucidate this issue. In our study, 71 nosocomial isolates from 41 patients and 23 community-acquired isolates from 21 patients were genotyped. Enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) was performed. Band patterns were compared using similarity coefficients of Dice, Jaccard and simple matching. Strain similarity for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa; Strain typing; ERIC-PCR; Nosocomial infections; Health care infections.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200008

Propolis effect on streptozotocin-induced diabetic rats
Provedor de dados: 80
Autores: Sartori,DRS; Kawakami,CL; Orsatti,CL; Sforcin,JM.
Propolis is one of the hive products that has been used extensively in folk medicine, due to its several biological and pharmacological properties. Besides, propolis-containing products have been intensely marketed by the pharmaceutical industry and health-food stores. This work was carried out in order to investigate whether propolis treatment could revert the metabolic alterations of streptozotocin-induced diabetic rats. Animals were kept in metabolic cages and diabetes was induced by a single dose of streptozotocin (35 mg/kg, IV). After a week, rats with glycemia higher than 230 mg/dL were divided into two groups and treated with ethanolic extract of propolis (10 and 90 mg/kg, PO) for seven days. Glycemia and free fatty acids were determined, as well as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian propolis; Diabetes; Glucose; Free fatty acids.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100009

Snake antivenoms: adverse reactions and production technology
Provedor de dados: 80
Autores: Morais,VM; Massaldi,H.
Antivenoms have been widely used for more than a century for treating snakebites and other accidents with poisonous animals. Despite their efficacy, the use of heterologous antivenoms involves the possibility of adverse reactions due to activation of the immune system. In this paper, alternatives for antivenom production already in use were evaluated in light of their ability to minimize the occurrence of adverse reactions. These effects were classified according to their molecular mechanism as: anaphylactic reactions mediated by IgE, anaphylactoid reactions caused by complement system activation, and pyrogenic reactions produced mainly by the presence of endotoxins in the final product. In the future, antivenoms may be replaced by humanized antibodies,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake antivenom; Anaphylactic reaction; Complement system activation; Endotoxins.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100002

Jorge Lobo's disease and its relation with antigens of the hla system
Provedor de dados: 80
Autores: Marcos,E. V. C.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100013

4-Phenyl-1,3-thiazole-2-amines as scaffolds for new antileishmanial agents
Provedor de dados: 80
Autores: Rodrigues,Carina Agostinho; Santos,Paloma Freire dos; Costa,Marcela Oliveira Legramanti da; Pavani,Thais Fernanda Amorim; Xander,Patrícia; Geraldo,Mariana Marques; Mengarda,Ana; Moraes,Josué de; Rando,Daniela Gonçales Galasse.
Abstract  Background: There is still a need for new alternatives in pharmacological therapy for neglected diseases, as the drugs available show high toxicity and parenteral administration. That is the case for the treatment of leishmaniasis, particularly to the cutaneous clinical form of the disease. In this study, we present the synthesis and biological screening of eight 4-phenyl-1,3-thiazol-2-amines assayed against Leishmania amazonensis. Herein we propose that these compounds are good starting points for the search of new antileishmanial drugs by demonstrating some of the structural aspects which could interfere with the observed activity, as well as suggesting potential macromolecular targets.  Methods: The compounds were easily synthesized by the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2-aminothiazoles; Antikinetoplastids; Antileishmanial; Cutaneous; Target fishing.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100317

Destruction of immunobiologicals and their disposal
Provedor de dados: 80
Autores: Gatti,MAN; Cardoso,SNM; Simeão,SFAP; Vitta,A; Monteiro,LM.
The guidelines of the Brazilian National Health System (SUS) state that the municipal authorities are responsible for the vaccination of the population. The present study examined the types of immunobiologicals, amounts and reasons for their destruction and disposal by the Municipal Health Secretariat in the city of Bauru, São Paulo state, Brazil, between 2008 and 2009. This study comprises a descriptive, exploratory and retrospective work that employed search of bibliographic data, collection of secondary data from forms of immunobiologicals disposal and interview of the agent responsible for the disposal of these products in the municipality. It was observed that the total numbers of unused vaccines in 2008 and 2009 were similar (4523 and 4395,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunization; Biologic waste disposal; Health services.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400017

Polymerase chain reaction in detecting Leishmania sp in symptomatic and asymptomatic seropositive dogs
Provedor de dados: 80
Autores: Soares,M. J. V.; Moraes,J. R. E.; Roselino,A. M. F.
In human and canine renal histological studies of visceral leishmaniasis (VL), the etiological agent is rarely found in situ. The objective of this study was to evaluate PCR in identifying the etiological agent in spleen, liver, lymph node, and kidneys of VL-seropositive dogs. Twenty-five symptomatic (case group) and 15 asymptomatic (control group) VL-seropositive dogs of different breeds, sexes, and ages from Teresina, Piauí State, Brazil, were used. Serologic diagnosis was made by enzyme-linked immunosorbent assay and indirect immunofluorescence test. Animals were subjected to euthanasia and necropsy. Renal fragments were immersed in buffered formaldehyde solution. Spleen, liver, lymph node, and kidney samples were collected and frozen at -70ºC until DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dogs; Leishmania; PCR; Kidney.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400011

Heterophilic antibodies in sera from individuals without loxoscelism cross-react with phospholipase D from the venom of Loxosceles and Sicarius spiders
Provedor de dados: 80
Autores: Arán-Sekul,Tomás; Rojas,José M.; Subiabre,Mario; Cruz,Victoria; Cortés,William; Osorio,Luis; González,Jorge; Araya,Jorge E.; Catalán,Alejandro.
Abstract  Background  Loxoscelism is a severe human envenomation caused by Loxosceles spider venom. To the best of our knowledge, no study has evaluated the presence of antibodies against Loxosceles venom in loxoscelism patients without treatment with antivenom immunotherapy. We perform a comparative analysis for the presence of antibodies capable of recognizing Loxosceles venom in a group of patients diagnosed with loxoscelism and in a group of people without loxoscelism.  Methods  The detection of L. laeta venom, Sicarius venom and recombinant phospholipases D from Loxosceles (PLDs) in sera from people with loxoscelism (Group 1) and from healthy people with no history of loxoscelism (Group 2) was evaluated using immuno-dot blot, indirect ELISA, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Loxosceles laeta; Heterophilic antibodies; Natural antibodies; Sicarius.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100311

Embryotoxicity following repetitive maternal exposure to scorpion venom
Provedor de dados: 80
Autores: Hmed,BN; Riadh,B; Serria,H; Kamel,J; Khaled,Z.
Although it is a frequent accident in a few countries, scorpion envenomation during pregnancy remains scarcely studied. In the present study, the effects of repetitive maternal exposure to Buthus occitanus tunetanus venom are investigated and its possible embryotoxic consequences on rats. Primigravid rats received a daily intraperitoneal dose of 1 mL/kg of saline solution or 300 µg/kg of crude scorpion venom, from the 7th to the 13th day of gestation. On the 21st day, the animals were deeply anesthetized using diethyl-ether. Then, blood was collected for chemical parameter analysis. Following euthanasia, morphometric measurements were carried out. The results showed a significant increase in maternal heart and lung absolute weights following venom...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prenatal repetitive exposure; Scorpions; Venom; Rats.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300009

Reversible atrial fibrillation following Crotalinae envenomation
Provedor de dados: 80
Autores: Quan,Dan; Zurcher,Kenneth.
Abstract  Background Cardiotoxicity is a documented complication of Crotalinae envenomation. Reported cardiac complications following snake envenomation have included acute myocardial infarction, electrocardiogram abnormalities and arrhythmias. Few reports exist describing arrhythmia induced by viper envenomation and to our knowledge none describe arrhythmia induced by Crotalinae envenomation. This report concerns the first known case of atrial fibrillation precipitated by rattlesnake bite.  Case presentation A 73-year-old Caucasian man with a past medical history of hypertension, hyperlipidemia, type 1 diabetes mellitus, and a baseline first-degree atrioventricular block presented to the emergency department following a rattlesnake bite to his left lower...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Crotalidae; Rattlesnake; Bite; Envenomation; Snakebite; Atrial fibrillation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100503

Diagnosis of Giardia infections by PCR-based methods in children of an endemic area
Provedor de dados: 80
Autores: David,EB; Coradi,ST; Oliveira-Sequeira,TCG; Ribolla,PEM; Katagiri,S; Guimarães,S.
The present study was designed to estimate the prevalence of Giardia infection in preschool- and school-aged children living in an endemic area. Fecal samples from 573 children were processed by zinc sulfate centrifugal flotation, centrifugal sedimentation (using a commercial device for fecal concentration - TF-Test kit®) and polymerase chain reaction (PCR)-based methods. Of the stool samples assessed, 277 (48.3%) were positive for intestinal parasites and/or commensal protozoa. Centrifugal flotation presented the highest diagnostic sensitivity for Giardia infections. The kappa index revealed that both coproparasitological techniques closely agreed on the Giardia diagnosis (86%) versus satisfactory (72%) and poor (35%) concordances for commensal protozoan...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Giardia duodenalis; Children; Diagnosis; PCR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200012

Control of ophidism in Brazil: a model for Africa
Provedor de dados: 80
Autores: Chippaux,J-P.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200001

Detection of Helicobacter pylori in gastric biopsies, saliva and dental plaques of dyspeptic patients from Marília, São Paulo, Brazil: presence of vacA and cagA genes
Provedor de dados: 80
Autores: Rasmussen,LT; Labio,RW de; C Neto,A; Silva,LC; Queiroz,VF; Smith,MAC; Payão,SLM.
Helicobacter pylori, a gram-negative bacterium, possesses two important virulence factors: the vacuolating toxin (vacA), and the cytotoxin-associated gene product (cagA). The aim of the present study was to evaluate the presence of H. pylori in the stomach and oral cavity of humans and compare the cagA and vacA genotypes of H. pylori found in different samples (stomach, saliva and dental plaque) from the same patient. Gastric biopsies, saliva and dental plaques were obtained from 62 dyspeptic adults. DNA was extracted and evaluated for the presence of H. pylori and the alleles cagA and vacA. Persons with gastritis had a higher frequency of H. pylori -positive samples in the stomach while positive samples from gastric biopsies were significantly correlated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; CagA; VacA; Dental plaque; Dyspeptic patient; Saliva.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200008

Cytotoxic effects of Pseudocerastes persicus venom and its HPLC fractions on lung cancer cells
Provedor de dados: 80
Autores: Shahbazi,Benyamin; Najafabadi,Zahra Salehi; Goudarzi,Hamidreza; Sajadi,Mahnaz; Tahoori,Fatemeh; Bagheri,Masoumeh.
ABSTRACT  Background:  Several studies have pointed out that certain snake venoms contain compounds presenting cytotoxic activities that selectively interfere with cancer cell metabolism. In this study, Pseudocerastes persicus venom and its fractions were investigated for their anticancer potential on lung cancer cells.  Methods: Lung cancer cells (A549) and normal fibroblast cells (Hu02) were treated with the P. persicus venom and its HPLC fractions and the cell cytotoxic effects were analyzed using MTT and lactate dehydrogenase release assays. Apoptosis was determined in venom-treated cell cultures using caspase-3 and caspase-9 assay kits.  Results:  The treatment of cells with HPLC fraction 21 (25-35 kDa) of P. persicus venom resulted in high LDH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudocerastes persicus venom; Persian horned viper; HPLC fractions; Cytotoxicity; Apoptosis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100319

Mad, bad and dangerous to know: the biochemistry, ecology and evolution of slow loris venom
Provedor de dados: 80
Autores: Nekaris,K Anne-Isola; Moore,Richard S; Rode,E Johanna; Fry,Bryan G.
Only seven types of mammals are known to be venomous, including slow lorises (Nycticebus spp.). Despite the evolutionary significance of this unique adaptation amongst Nycticebus, the structure and function of slow loris venom is only just beginning to be understood. Here we review what is known about the chemical structure of slow loris venom. Research on a handful of captive samples from three of eight slow loris species reveals that the protein within slow loris venom resembles the disulphide-bridged heterodimeric structure of Fel-d1, more commonly known as cat allergen. In a comparison of N. pygmaeus and N. coucang, 212 and 68 compounds were found, respectively. Venom is activated by combining the oil from the brachial arm gland with saliva, and can...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venoms; Ecology; Primates; Intraspecific competition; Predation; Ectoparasite; Naja naja.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100203

Intraspecific diversity of scorpions venom and its implication in the pathophysiological effects
Provedor de dados: 80
Autores: Abdel-Rahman,Mohamed A.
Tipo: Info:eu-repo/semantics/other
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100020

Evaluation of the neutralizing capacity of Androctonus crassicauda (Olivier, 1807) antivenom against Leiurus quinquestriatus (Ehrenberg, 1928) venom (Scorpiones: Buthidae)
Provedor de dados: 80
Autores: Ozkan,O; Adiguzel,S; Inceoglu,B; Lango,J; Ertek,M; Hammock,BD.
The two most venomous species of the family Buthidae, Leiurus quinquestriatus and Androctonus crassicauda, are found in Africa and in the Middle East. Potency and paraspecific activities of A. crassicauda antivenom (RSHC anti-Ac) were tested against L. quinquestriatus venom. The sera produced by Refik Saydam Hygiene Center (RSHC) showed strong reactivity against the venoms of A. crassicauda and L. quinquestriatus in western blotting and dot-blot analysis. RSHC anti-Ac presents immunoactivity and neutralizing potential against Leiurus quinquestriatus venom. Neutralization capacity of antivenom was found to be 400 µL against 40 minimum lethal doses (MLD) of A. crassicauda scorpion venom and 10 MLD of L. quinquestriatus venom. This study indicates that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion; Androctonus crassicauda; Leiurus quinquestriatus; Venom; Monovalent antivenom; Paraspecific activity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300008

It is time for top-down venomics
Provedor de dados: 80
Autores: Melani,Rafael D.; Nogueira,Fabio C. S.; Domont,Gilberto B.
Abstract The protein composition of animal venoms is usually determined by peptide-centric proteomics approaches (bottom-up proteomics). However, this technique cannot, in most cases, distinguish among toxin proteoforms, herein called toxiforms, because of the protein inference problem. Top-down proteomics (TDP) analyzes intact proteins without digestion and provides high quality data to identify and characterize toxiforms. Denaturing top-down proteomics is the most disseminated subarea of TDP, which performs qualitative and quantitative analyzes of proteoforms up to ~30 kDa in high-throughput and automated fashion. On the other hand, native top-down proteomics provides access to information on large proteins (&gt; 50 kDA) and protein interactions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venomics; Toxiforms; Top-down proteomics; Denaturing top-down proteomics; Native top-down proteomics.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100209

Outbreaks of Ebola virus disease in Africa: the beginnings of a tragic saga
Provedor de dados: 80
Autores: Chippaux,Jean-Philippe.
The tremendous outbreak of Ebola virus disease occurring in West Africa since the end of 2013 surprises by its remoteness from previous epidemics and dramatic extent. This review aims to describe the 27 manifestations of Ebola virus that arose after its discovery in 1976. It provides an update on research on the ecology of Ebola viruses, modes of contamination and human transmission of the disease that are mainly linked to close contact with an infected animal or a patient suffering from the disease. The recommendations to contain the epidemic and challenges to achieve it are reminded.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ebola; Outbreak; Virus; Hemorrhagic fever; Africa.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200209

Visceral leishmaniasis in children from Mato Grosso do Sul state, Brazil: a contribution to diagnosis and treatment
Provedor de dados: 80
Autores: Brustoloni,Y. M.
Tipo: Info:eu-repo/semantics/article
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200013

Clinical and laboratory features distinguishing between Deinagkistrodon acutus and Daboia siamensis envenomation
Provedor de dados: 80
Autores: Su,Hung-Yuan; Huang,Shih-Wei; Mao,Yan-Chiao; Liu,Ming-Wen; Lee,Kuo-Hsin; Lai,Pei-Fang; Tsai,Ming-Jen.
Abstract  Background: There are 6 species of venomous snakes in Taiwan. Two of them, Deinagkistrodon acutus (D. acutus) and Daboia siamensis (D. siamensis), can cause significant coagulopathy. However, a significant proportion of patients with snakebites cannot identify the correct snake species after envenomation, which hampers the application of antivenom. Hence, the differential diagnosis between the two snakebites by clinical presentations is important. This study aims to compare their clinical and laboratory features for the purpose of differential diagnosis between the two snakebites.  Methods: We retrospectively reviewed the medical records of patients who arrived at the emergency department due to D. acutus or D. siamensis envenomation, between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coagulopathy; Deinagkistrodon acutus; Daboia siamensis; Snakebite; Thrombocytopenia.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100333

Indian scorpions collected in Karnataka: maintenance in captivity, venom extraction and toxicity studies
Provedor de dados: 80
Autores: Nagaraj,Santhosh Kambaiah; Dattatreya,Pavana; Boramuthi,Thippeswamy Nayaka.
Abstract  Background  Maintenance of scorpions under laboratory conditions is ideal for long-term venom collection to explore the therapeutic applications of scorpion venom. Collection of venom by electrical stimulation requires a reliable stimulator and effective restrainer. Thus, the present study was conducted to develop a convenient method to maintain scorpions and to extract their venom for toxicity studies via a modified restrainer and stimulator.  Methods  Four different scorpion species were collected, among which three species were maintained in the laboratory in containers that mimic their natural habitat. Venom was extracted from Hottentotta rugiscutis by electrical stimulation at 8 V for 18 months and LD50 was estimated by the graphic method of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venom Restrainer; Hottentotta Heterometrus; Venom extraction LD50.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100354

Dangerous snakes, deadly snakes and medically important snakes
Provedor de dados: 80
Autores: Silva,Anjana.
This correspondence argues that the dangerousness of a venomous snake species is not solely determined by the venom characteristics or the lethality of the snake, and recognizes that medical importance comprises a key variable as well. The medical importance of a snake is determined by several factors - including frequency of medical attention after a bite, local or systemic envenomation provoked by the bite, fatal bites, long term consequences, availability of antivenom therapy as well as the size of the population at risk - that may vary from one region to another.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Snakes; Snake bites; Snake venoms; Snake envenomation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100102

Ocellatin peptides from the skin secretion of the South American frog Leptodactylus labyrinthicus (Leptodactylidae): characterization, antimicrobial activities and membrane interactions
Provedor de dados: 80
Autores: Gusmão,Karla A. G.; Santos,Daniel M. dos; Santos,Virgílio M.; Cortés,María Esperanza; Reis,Pablo V. M.; Santos,Vera L.; Piló-Veloso,Dorila; Verly,Rodrigo M.; Lima,Maria Elena de; Resende,Jarbas M.
Abstract  Background  The availability of antimicrobial peptides from several different natural sources has opened an avenue for the discovery of new biologically active molecules. To the best of our knowledge, only two peptides isolated from the frog Leptodactylus labyrinthicus, namely pentadactylin and ocellatin-F1, have shown antimicrobial activities. Therefore, in order to explore the antimicrobial potential of this species, we have investigated the biological activities and membrane interactions of three peptides isolated from the anuran skin secretion.  Methods  Three peptide primary structures were determined by automated Edman degradation. These sequences were prepared by solid-phase synthesis and submitted to activity assays against gram-positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptodactylus labyrinthicus; Ocellatins; Antimicrobial peptides; Peptide membrane interactions.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100302

Hepatitis B virus surface antigen seroconversion in HIV-infected individual after pegylated interferon-alpha treatment: a case report
Provedor de dados: 80
Autores: Delicio,Adriane Maira; Abati,Paulo Afonso Martins; Vigani,Aline Gonzalez.
Hepatitis B virus (HBV) infects from 6 to 14% of HIV-infected individuals. Concurrent HIV/HBV infection occurs due to the overlapping routes of transmission, particularly sexual and parenteral. HIV-infected patients that have acute hepatitis B have six times greater risk of developing chronic hepatitis B, with higher viral replication, rapid progression to end-stage liver disease and shorter survival. The coinfection is also associated with poor response to hepatitis B treatment with interferon-alpha and increased liver toxicity to the antiretroviral therapy. Herein, we describe the case of a 35-year-old man who engages in sex with men and presented with newly diagnosed HIV-1, serological markers for acute hepatitis B and progression to chronic hepatitis B...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: HIV; Hepatitis B; HBsAg; Peginterferon.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100503

Biochemical characterization and cytotoxic effect of the skin secretion from the red-spotted Argentina frog Argenteohyla siemersi (Anura: Hylidae)
Provedor de dados: 80
Autores: Fusco,Luciano S.; Cajade,Rodrigo; Piñeiro,Jose M.; Torres,Ana M.; Silva,Igor R. F. da; Hyslop,Stephen; Leiva,Laura C.; Pimenta,Daniel C.; Bustillo,Soledad.
Abstract  Background: Argenteohyla siemersi (red-spotted Argentina frog) is a casque-headed tree frog species belonging to the Hylidae family. This species has a complex combination of anti-predator defense mechanisms that include a highly lethal skin secretion. However, biochemical composition and biological effects of this secretion have not yet been studied.  Methods: The A. siemersi skin secretion samples were analyzed by mass spectrometry and chromatographic analysis (MALDI-TOF/MS, RP-HPLC and GC-MS). Proteins were also studied by SDS-PAGE. Among the biological activities evaluated, several enzymatic activities (hemolytic, phospholipase A2, clotting, proteolytic and amidolytic) were assessed. Furthermore, the cytotoxic activity (cytolysis and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Argenteohyla siemersi; Hylidae; Skin secretion; Tree frog; Cytotoxicity.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100306

Kn-Ba: a novel serine protease isolated from Bitis arietans snake venom with fibrinogenolytic and kinin-releasing activities
Provedor de dados: 80
Autores: Megale,Ângela Alice Amadeu; Magnoli,Fábio Carlos; Kuniyoshi,Alexandre Kazuo; Iwai,Leo Kei; Tambourgi,Denise V.; Portaro,Fernanda C. V.; Silva,Wilmar Dias da.
Abstract  Background: Bitis arietans is a venomous snake found in sub-Saharan Africa and in parts of Morocco and Saudi Arabia. The envenomation is characterized by local and systemic reactions including pain, blistering, edema and tissue damage, besides hemostatic and cardiovascular disturbances, which can cause death or permanent disabilities in its victims. However, the action mechanisms that provoke these effects remain poorly understood, especially the activities of purified venom components. Therefore, in order to elucidate the molecular mechanisms that make the Bitis arietans venom so potent and harmful to human beings, this study reports the isolation and biochemical characterization of a snake venom serine protease (SVSP).  Methods: Solubilized...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bitis arietans; Venom; Antivenom; Serine protease; Fibrinogenolytic; Kinin-releasing activity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100328

Clostridium perfringens types A and D associated with enterotoxemia in an 18-month-old goat
Provedor de dados: 80
Autores: Miyashiro,S.; Nassar,A. F. C.; Del Fava,C.; Cabral,A. D.; Silva,M.
Postmortem examination of a Boer buck that died peracutely revealed bowel and liver diffusely congested and edematous. Kidney was apparently edematous. Clostridium perfringens type A was isolated from bowel and type D from kidney. Microscopic examination revealed large areas of necrosis in the renal cortex and medulla (pulpy kidney disease), hyperemia and centrilobular necrosis of the liver, necrosis of the small-intestine wall, pulmonary edema and congestion, intense hyperemia of the cerebellum, hyperemia and edema of the brain.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Goats; Enterotoxemia; Pulpy kidney disease; Clostridium perfringens type A; Clostridium perfringens type D; Histopathology.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400017

Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge
Provedor de dados: 80
Autores: Miyasato,PA; Ramos,CRR; Abreu,PAE; Dias,WO; Nascimento,C; Ho,PL; Kawano,T.
Schistosomes utilize proteinases to accomplish several activities such as tissue penetration, tissue digestion and evasion of host immune responses. Cathepsin L is a cysteine proteinase of the papain superfamily detected in their gut lumen which indicates that this enzyme contributes to the proteolysis of ingested hemoglobin. Due to the roles played in the schistosome biology, proteolytic enzymes are considered potential targets for developing and guiding antischistosomal therapies. In the present work, the cathepsin L1 cDNA coding of Schistosoma mansoni was cloned into the pAE vector that provides high-level expression of heterologous proteins in Escherichia coli. The recombinant protein was expressed as inclusion bodies, purified under denaturing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schistosoma mansoni; Cysteine proteinase; Cathepsin L1; Vaccine; Immunogenicity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200011

Hepatobiliary scintigraphy with 99mTc-DISIDA of pulmmonary tuberculosis patients in treatment with isonazid, rifampicin and pyrazinamide: relation with the nutrional state and the acetyletor phenotype
Provedor de dados: 80
Autores: Camargo,R. S. A. de.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100017

Detection of antibodies to Toxoplasma gondii in wild animals in Brazil
Provedor de dados: 80
Autores: Silva,Rodrigo Costa da; Machado,Gustavo Puglia; Cruvinel,Tatiane Morosini de Andrade; Cruvinel,Ciro Alexandre; Langoni,Helio.
Background Toxoplasmosis is a worldwide zoonosis caused by an obligate intracellular protozoan parasite,Toxoplasma gondii, that affects all warm-blooded animals, including wild animals. The increased number of cases of parasitic infections is mainly due to the destruction of environmental conservation areas, which is driving wild animals out of their habitats and towards urban areas. In this study, the occurrence of T. gondii infection was investigated by the modified agglutination test (MAT) in 26 different species of run over and injured wild animals that were treated at a Brazilian university veterinary hospital, from June 2007 to August 2008.Findings Of the studied animals, six (23.1%; CI95% 11.1-42.2%) had T. gondii antibodies, with titers equal to 10...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Natural infection; Antibodies; Wild animals; MAT.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200603

Antiviral activities of extracts and phenolic components of two Spondias species against dengue virus
Provedor de dados: 80
Autores: Silva,Ara; Morais,SM; Marques,MMM; Lima,DM; Santos,SCC; Almeida,RR; Vieira,IGP; Guedes,MIF.
In recent years, the search for natural plant products to fight viral diseases has been increasing. In this work, two Spondias species, namely S. mombin and S. tuberosa, found in Ceará state (Brazil), and their main phenolic components were evaluated against dengue virus. In vitro antiviral tests were performed against type-2 dengue virus by the MTT method and standard cytopathic effect reduction assay in C6/36 cells. Cytotoxicity was also evaluated by MTT. The presence of phenolic compounds quercetin, rutin, and ellagic acid in plant extracts was characterized by HPLC analysis. Both Spondias species extracts and components were nontoxic to the cells whereas rutin and quercetin displayed relevant antiviral activity with IC50 of 362.68 µg/mL and 500 µg/mL,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Natural products; Phenols; Dengue; Antiviral agents.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400007

Structure of nematocysts isolated from the fire corals Millepora alcicornis and Millepora complanata (Cnidaria: Hydrozoa)
Provedor de dados: 80
Autores: García-Arredondo,A; Rojas,A; Iglesias-Prieto,R; Zepeda-Rodriguez,A; Palma-Tirado,L.
Structural characteristics of discharged and undischarged nematocysts from the hydrozoans Millepora alcicornis and Millepora complanata, two fire corals collected in the Mexican Caribbean, were examined using transmission electron, scanning and light microscopy. In this study, we report for the first time images of the nematocysts found in these Mexican Caribbean venomous species. Two types of nematocysts were observed in both species, the more abundant identified as macrobasic mastigophore and the other a stenotele type. Macrobasic mastigophores were present in medium and large size classes while stenoteles appeared in only one size.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrocoral; Nematocysts; Microscopy images; Macrobasic mastigophore; Stenotele.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100014

Effect of bee venom on IL-6, COX-2 and VEGF levels in polycystic ovarian syndrome induced in Wistar rats by estradiol valerate
Provedor de dados: 80
Autores: Karimzadeh,Latifeh; Nabiuni,Mohammad; Kouchesfehani,Homa Mohseni; Adham,Hamed; Bagheri,Amir; Sheikholeslami,Azar.
Background : Polycystic ovarian syndrome (PCOS) is a low-grade inflammatory disease characterized by hyperandrogenemia, hirsutism, chronic anovulation and vascular disorder. Interleukin-6 (IL-6), cyclooxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF) are triggered by inflammatory stimuli and lead to angiogenesis and pathogenesis of the ovary. Honeybee venom (HBV) contains an array of biologically active components possessing various pharmaceutical properties. This study was designed to assess the possibility of HBV application as an anti-inflammatory therapeutic agent to suppress levels of the main inflammatory mediators IL-6, COX-2 and VEGF. To induce PCOS, 1 mg of estradiol valerate (EV) per 100 g of body weight was subcutaneously (SC)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polycystic ovarian syndrome; Honeybee venom; Interlukin-6; Cyclooxygenase-2; Vascular endothelial growth factor.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100317

Comparison of anti-rabies titles in bovines submitted to five distinct vaccination schedules
Provedor de dados: 80
Autores: Albas,A.
Tipo: Info:eu-repo/semantics/other
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100014

Initial peptidomic profiling of Brazilian sea urchins: Arbacia lixula, Lytechinus variegatus and Echinometra lucunter
Provedor de dados: 80
Autores: Sciani,Juliana Mozer; Emerenciano,Andrews Krupinski; Silva,José Roberto Machado Cunha da; Pimenta,Daniel Carvalho.
Abstract  Background  Sea urchins can be found throughout the Brazilian coast and are reported to be one of the major causes of marine accidents on the shoreline. Although not lethal, these accidents are reported to be extremely painful. In order to understand the toxinology of the Brazilian urchins, a peptidomic approach was performed aiming to characterize the naturally occurring peptides in both the coelomic fluid and the spine.  Methods  Animals were collected without gender distinction and samples of the coelomic fluid and spines extracted were analyzed by RP-HPLC and mass spectrometry for peptide de novo sequencing.  Results  Several peptides were identified either in the coelomic fluid or the spine extract (except for E. lucunter). The peptide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peptides; Toxins; Sea urchin; Peptidomics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100313

Predictive determinants of scorpion stings in a tropical zone of south Iran: use of mixed seasonal autoregressive moving average model
Provedor de dados: 80
Autores: Ebrahimi,Vahid; Hamdami,Esmael; Moemenbellah-Fard,Mohammad Djaefar; Jahromi,Shahrokh Ezzatzadegan.
Abstract  Background: More than 1.2 million scorpion stings occur annually worldwide, particularly in tropical regions. In the absence of proper medical care, mortality due to venomous scorpion stings is an important public health issue. The aim of the present study is to explore the temporal trend of scorpionism with time series models and determine the effective factors on this event using regression models.  Methods: A retrospective cross sectional study was conducted on 853 scorpion stung patients. They were referred to Haji-Abad Hospital of Hormozgan University of Medical Sciences (HUMS), south Iran, from May 2012 to July 2016. A linear model to describe and predict the monthly trend of scorpion sting cases is fit with autoregressive moving average...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Climate; Mixed seasonal ARMA; Regression analysis; Scorpion; Scorpion stings; Time series; Iran.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100315

Venomous and poisonous animals
Provedor de dados: 80
Tipo: Info:eu-repo/semantics/article
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200012

"Vitalis Brazilis", burning embers, beyond the ashes, at the Butantan Institute
Provedor de dados: 80
Autores: De Lima,ME.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300005

Evaluation of Histoplasma capsulatum infection in inhabitants of the urban and rural areas of Pratânia, São Paulo, by means of intradermal reactions
Provedor de dados: 80
Autores: Rodrigues,C. C.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200011

Ecological studies on phlebotominae (diptera:psychodidae) in an urban area of the city of Campo Grande, Mato Grosso do sul State, Brazil
Provedor de dados: 80
Autores: Oliveira,Alessandra Gutierrez de.
Tipo: Info:eu-repo/semantics/article
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100013

Effect of fibrin glue derived from snake venom on the viability of autogenous split-thickness skin graft
Provedor de dados: 80
Autores: Rahal,S.C.; Amaral,M.S.P.; Pai,V.D.; Barraviera,S.R.C.S.; Caporal,E.H.G; Crocci,A.J.
The aim of this study was to analyze the effect of snake venom derived from fibrin glue on the viability of split-thickness skin graft. Nine crossbreed dogs were used. Full-thickness skin segments measuring 4 x 4 cm were bilaterally excised from the proximal radial area on each dog. A split-thickness skin graft was harvestedfrom left lateral thoracic area using a freehand graft knife, and was secured to the left recipient bed using several simple interrupted sutures of 3-0 nylon (sutured graft). A split-thickness skin graft was harvested from the right lateral thoracic area using a graft knife. Fibrin glue derived from snake venom was applied to the recipient bed, and 8 equidistant simple interrupted sutures of 3-0 nylon were used to secure the skin graft...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibrin glue; Skin graft; Snake venom; Dogs.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200006

Poster
Provedor de dados: 80
Tipo: Info:eu-repo/semantics/article
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200014

Use of fibrin glue derived from snake venom in the repair of deep corneal ulcers: experimental study in dogs (Canis familiaris, Linnaeus, 1758)
Provedor de dados: 80
Autores: Sampaio,R. L.; Ranzani,J. J. T.; Brandão,C. V. S.; Thomazini-Santos,I. A.; Barraviera,B.; Barraviera,S. R. C. S.; Giannini,M. J. S. M.
Fibrin glue has been researched as an alternative method for tissue synthesis and is known for its capability to promote hemostasis at the application site, good approximation of wound edges and fast healing. The current study consisted in the application of fibrin glue derived from snake venom as treatment for experimental corneal ulcers. Twenty-one dogs had their corneas experimentally prepared through lamellar keratectomy (of standardized diameter and depth). Animals were divided into seven groups of three animals each. Six experimental groups were periodically evaluated and collection was carried out on the 1st, 3rd, 7th, 15th, 30th and 60th post-operative days, whereas one control group was evaluated throughout the experiment. Analyses consisted in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibrin glue; Snake venom; Cornea; Corneal ulcer; Keratoplasty.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400014

Predictors of incorrectness in antimicrobial prescription in the Bauru State Hospital (Bauru city, São Paulo State, Brazil)
Provedor de dados: 80
Autores: Kawanami,G. H.
Tipo: Info:eu-repo/semantics/review
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200019

In vitro activity of phospholipase A 2 and of peptides from Crotalus durissus terrificus venom against amastigote and promastigote forms of Leishmania (L.) infantum chagasi
Provedor de dados: 80
Autores: Barros,Gustavo A. C.; Pereira,Andreia V.; Barros,Luciana C.; Lourenço Jr,Airton; Calvi,Sueli A.; Santos,Lucilene D.; Barraviera,Benedito; Ferreira Jr,Rui Seabra.
Abstract  Background  American visceral leishmaniasis is caused by the intracellular parasiteLeishmania (L.) infantum chagasi, and transmitted by the sand fly Lutzomyia longipalpis. Since treatment is based on classical chemotherapeutics with significant side effects, the search for new drugs remains the greatest global challenge. Thus, this in vitro study aimed to evaluate the leishmanicidal effect ofCrotalus durissus terrificus venom fractions on promastigote and amastigote forms of Leishmania (L.) infantum chagasi.  Methods  Phospholipase A 2 (PLA 2 ) and a pool of peptide fraction (&lt;3 kDa) were purified from Crotalusvenom. Furthermore, promastigotes and peritoneal macrophages of mice infected by amastigotes were exposed to serial dilutions of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PLA2; Peptides; Crotalus dutissus terrificus; Venom; Leishmanicidal activity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100351

Clinical and morphological evaluation of snake venom derived fibrin glue on the tendon healing in dogs
Provedor de dados: 80
Autores: Ferraro,G. C.; Moraes,J. R. E.; Pereira,G. T.; Moraes,F. R.; Bueno de Camargo,M. H.
The aim of this study was to evaluate the effect of snake venom derived fibrin glue on the healing of the deep digital flexor tendon, during three periods. The tendon of the 2nd digit of 30 thoracic limbs of dogs was partially sectioned for glue application. Biopsies were performed 7, 15, and 30 days post surgery for the clinical and morphological study of tendons. Analysis of the results showed that 73.3% of the tendons showed stump retraction and 16.6% moderate to excessive adherence, which affected sliding. There was a significant difference in the number of inflammatory cells among the three studied periods, being the highest on day 15. The morphological analysis revealed a typical tendon healing process with a lower level of inflammation in the acute...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dog; Fibrin glue; Snake venom; Healing; Tendon.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400005

Enzymatic and immunological properties of Bungarus flaviceps (red-headed krait) venom
Provedor de dados: 80
Autores: Tan,NH; Fung,SY; Ponnudurai,G.
Bungarus flaviceps (red-headed krait) venom presents an intravenous LD50 of 0.32 μg/g and exhibits enzymatic activities similar to other Bungarus toxins. ELISA cross-reactions between anti-Bungarus flaviceps and a variety of elapid and viperid venoms were observed in the current study. Double-sandwich ELISA was highly specific, since anti-B. flaviceps serum did not cross-react with any tested venom, indicating that this assay can be used for species diagnosis in B. flaviceps bites. In the indirect ELISA, anti-B. flaviceps serum cross-reacted moderately with three different Bungarus venoms (9-18%) and Notechis scutatus venom, but minimally with other elapid and viperid toxins. The results indicated that B. flaviceps venom shares common epitopes with other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bungarus flaviceps venom; Enzymes; ELISA; Neutralization.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100014

Traceability of animal protein byproducts in ruminants by multivariate analysis of isotope ratio mass spectrometry to prevent transmission of prion diseases
Provedor de dados: 80
Autores: Ferreira Jr.,Rui Seabra; Silva,Daniela Alessandra Fossato da; Biscola,Natália Perussi; Sartori,Maria Márcia Pereira; Denadai,Juliana Célia; Jorge,André Mendes; Santos,Lucilene Delazari dos; Barraviera,Benedito.
Abstract  Background: Ruminant feed containing animal byproduct proteins (ABPs) is prohibited in many countries due to its risk of transmitting prion diseases (PD). In most cases the entire herd is sacrificed, which causes great harm to the producer countries by preventing their exportation of ruminant derived-products.  Methods: We used stable isotope ratio mass spectrometry (IRMS) of carbon (13C/12C) and nitrogen (15N/14N) to trace the animal protein in the blood of 15 buffaloes (Bubalus bubalis) divided into three experimental groups: 1 - received only vegetable protein (VP) during 117 days; 2 - received animal and vegetable protein (AVP); and 3 - received animal and vegetable protein with animal protein subsequently removed (AVPR). Groups 2 and 3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prion diseases; Monitoring; Bovine spongiform; Encephalopathy; Stable isotopes.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100312

Courtship and mating of Scorpiops luridus Zhu Lourenço &amp;amp; Qi, 2005 (Scorpiones: Euscorpiidae) from Xizang province, China
Provedor de dados: 80
Autores: Jiao,GB; Zhu,MS.
In the current work, the courtship and mating of Scorpiops luridus Zhu Lourenço &amp;amp; Qi, 2005 (Euscorpiidae) from Xizang province (Tibet), China, were studied for the first time in the laboratory. Most of the mating behaviors in Scorpiops luridus are not remarkably different from those exhibited by other scorpions. However, for the first time a male pulling a female with its chelicerae to rapidly accomplish the sperm uptake was observed. Additionally, the sexual stinging behavior displayed by the male occurred in the initial stage, not during the promenade stage as previously described in several scorpion species. Through observation and analysis, we speculate that venom injection during sexual stinging is selective, possibly relying on the status...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion; Scorpiops; Courtship; Mating; Behavior.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100015

Pediatric suppurative parotitis caused by Burkholderia pseudomallei
Provedor de dados: 80
Autores: Fu,Zengzhen; Lin,Yingzi; Wu,Qiang; Xia,Qianfeng.
Abstract  Background: Suppurative parotitis caused by Burkholderia pseudomallei has been rarely found outside endemic areas.  Case presentation: Herein, we report the recovery of Burkholderia pseudomallei from the pus of a suppurative parotitis observed in a 12-year-old boy who lived in Hainan province, China. Specimens of necrotic tissue were collected and sections were stained with hematoxylin and eosin. Pus sample was also collected for bacteriological examination. The suppurative inflammation was observed in the necrotic tissue section and Burkholderia pseudomallei were detected in the sample.  Conclusion: In this adolescent, Burkholderia pseudomallei infection was present in the parotid, which consists of the first report of this bacterium in a...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Melioidosis; Parotitis; Burkholderia pseudomallei; Pediatrics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100504

Effect of selected anti-inflammatory drugs on the lethal actions of Leiurus quinquestriatus venom
Provedor de dados: 80
Autores: Abdoon,N. A.; Ali,A. A.; Alnema,A. A.; Hag-Ali,M.; Fatani,A. J.
The cumulative actions of scorpion neurotoxins are complex and may be traced to activation of different ion channels with subsequent release of various transmitters and modulators including inflammatory mediators. This could lead to various pathological manifestations such as acute respiratory distress syndrome (ARDS), systemic inflammatory response syndrome (SIRS), and multiple organ failure (MOF). Several approaches have been advocated to treat the multitude of scorpion-venom-elicited pathological changes. However, few have tried to combat the venom-induced effects on the inflammatory process, which manifest as ARDS, SIDS and MOF. Thus, the aim of this study was to determine the capability of inhibitors of different steps of the inflammatory sequence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leiurus quinquestriatus quinquestriatus; Scorpion venom; Acute respiratory distress syndrome; Systemic inflammatory response syndrome; Multiple organ failure; Montelukast; Hydrocortisone; Indomethacin.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300003

Neuroactive compounds obtained from arthropod venoms as new therapeutic platforms for the treatment of neurological disorders
Provedor de dados: 80
Autores: Monge-Fuentes,Victoria; Gomes,Flávia Maria Medeiros; Campos,Gabriel Avohay Alves; Silva,Juliana de Castro; Biolchi,Andréia Mayer; Anjos,Lilian Carneiro dos; Gonçalves,Jacqueline Coimbra; Lopes,Kamila Soares; Mortari,Márcia Renata.
The impact of neurological disorders in society is growing with alarming estimations for an incidence increase in the next decades. These disorders are generally chronic and can affect individuals early during productive life, imposing real limitations on the performance of their social roles. Patients can have their independence, autonomy, freedom, self-image, and self-confidence affected. In spite of their availability, drugs for the treatment of these disorders are commonly associated with side effects, which can vary in frequency and severity. Currently, no effective cure is known. Nowadays, the biopharmaceutical research community widely recognizes arthropod venoms as a rich source of bioactive compounds, providing a plethora of possibilities for the...
Tipo: Info:eu-repo/semantics/article
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100212

Public health aspects of snakebite care in West Africa: perspectives from Nigeria
Provedor de dados: 80
Autores: Habib,Abdulrazaq G.
Snakebite envenoming is a major public health problem among rural communities of the Nigerian savanna. The saw-scaled or carpet viper (Echis ocellatus) and, to a lesser extent, the African cobras (Naja spp.) and puff adders (Bitis arietans) have proved to be the most important cause of mortality and morbidity. The main clinical features of E. ocellatus envenoming are systemic hemorrhage, incoagulable blood, shock, local swelling, bleeding and, occasionally, necrosis. Bites may be complicated by amputation, blindness, disability, disfigurement, mutilation, tissue destruction and psychological consequences. Antivenom remains the hallmark and mainstay of envenoming management while studies in Nigeria confirm its protection of over 80% against mortality from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antivenom; Carpet viper; Envenoming; Hub-and-spoke; Nigeria; Snakebite.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100204

Triple bothropic envenomation in horses caused by a single snake
Provedor de dados: 80
Autores: Chiacchio,SB; Martins,GTB; Amorim,RM; Gonçalves,RC; Barraviera,B; Ferreira Junior,RS.
In the present study, snakebites caused by a single Bothrops pauloensis simultaneously affecting three male adult horses are described. Whereas the first two affected horses were six years old, the third was 16 years old; they weighed respectively 555, 550 and 500 kg. All horses presented swollen muzzles. The first animal received an initial antivenom dose of 19 vials and adjuvant medication, it was also subjected to tracheostomy due to the progression of acute respiratory failure. The second and third horses respectively received 15 vials of antivenom each, in addition to adjuvant medication. Due to continuous changes in blood coagulation observed during hospitalization, the first and the second horses received five more vials of antivenom, respectively,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops; Snakebite; Equine; Antivenom; Tracheostomy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100016

Integrity in scientific publication
Provedor de dados: 80
Autores: SM,Rode.
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200001

Using prodigiosin against some gram-positive and gram-negative bacteria and Trypanosoma cruzi
Provedor de dados: 80
Autores: Herráez,Rocío; Mur,Anna; Merlos,Alexandra; Viñas,Miguel; Vinuesa,Teresa.
Abstract  Background: This work aimed to explore the action of natural prodigiosin on both bacterial organisms and Trypanosoma cruzi cells.  Methods: Natural prodigiosin pigment was extracted and purified from cultures of Serratia marcescens. Two media, peanut broth and peptone glycerol broth, both recommended in the literature for prodigiosin production, were compared. The prodigiosin obtained was employed to explore its antimicrobial properties against both bacteria and Trypanosoma cruzi cells.  Results: Peanut broth yielded four times more prodigiosin. The prodigiosin showed remarkable activity (minimal inhibitory concentrations in the range of 2-8 µM for bacteria and half maximal inhibitory concentration of 0.6 µM for Trypanosoma cruzi). In fact, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Chagas disease; Prodigiosin; Atomic force microscopy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100314

Snakebite on the hand: lessons from two clinical cases illustrating difficulties of surgical indication
Provedor de dados: 80
Autores: Gras,S; Plantefève,G; Baud,F; Chippaux,JP.
Snakebite is a particularly important health problem in rural areas of tropical regions. A large number of victims survive with permanent physical sequelae due to local tissue necrosis. However, necrosis may be associated with compartment syndrome especially when the bite is on the hands or feet. Herein, we describe two cases reported at a rural district hospital in Central African Republic. The present study suggests that active multidisciplinary management may improve patient prognosis while evidencing how difficult it is to decide on surgical intervention.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Snakebite; Africa; Compartment syndrome; Surgery.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400019

Is the cat an important reservoir host for visceral leishmaniasis? A systematic review with meta-analysis
Provedor de dados: 80
Autores: Asfaram,Shabnam; Fakhar,Mahdi; Teshnizi,Saeed Hosseini.
Abstract In recent years feline leishmanial infections (FLI) have been studied more than ever before in various parts of the world. However, evidence-based knowledge on FLI has remained unavailable. The main objectives of this study were to investigate the status of felines infected by Leishmania spp. worldwide. Data were extracted from 10 available databases over the period of 1982 to 2017. Overall, 78 articles fulfilled the inclusion criteria and were used for data extraction in this systematic review. The overall FLI prevalence by both serological and molecular methods was estimated at 10% (95% CI: 8%-14%). In Italy, both the seroprevalence (24 %) and PCR prevalence (21 %) were found to be higher than in other countries. The most common diagnostic test...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feline leishmanial infection; Global prevalence; Diagnostic tests; Systematic review; Meta-analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100204

Oral microbiota of Brazilian captive snakes
Provedor de dados: 80
Autores: Fonseca,MG; Moreira,WMQ; Cunha,KC; Ribeiro,ACMG; Almeida,MTG.
The present work aimed to determine the oral microbiotic composition of snakes from São José do Rio Preto city, São Paulo State, Brazil. Ten snake species, comprising the families Boidae, Colubridae, Elapidae and Viperidae, were submitted to microbiological examination of their oral cavity, which indicated positivity for all buccal samples. Gram-negative bacilli, gram-negative cocci bacilli, gram-positive bacilli and gram-positive cocci were isolated from the snakes. Among isolated bacterium species, the occurrence of coagulase-negative staphylococci in the buccal cavity of Crotalus durissus (Viperiade), Eunectes murinus (Boidae), Mastigodryas bifossatus (Colubridae) and Bacillus subtilis, common to oral cavity of Bothrops alternatus (Viperidae) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venomous snakes; Non-venomous snakes; Oral cavity; Bacterial isolation; Captive snakes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100006

Protein-species quantitative venomics: looking through a crystal ball
Provedor de dados: 80
Autores: Calvete,Juan J.; Petras,Daniel; Calderón-Celis,Francisco; Lomonte,Bruno; Encinar,Jorge Ruiz; Sanz-Medel,Alfredo.
Abstract In this paper we discuss recent significant developments in the field of venom research, specifically the emergence of top-down proteomic applications that allow achieving compositional resolution at the level of the protein species present in the venom, and the absolute quantification of the venom proteins (the term “protein species” is used here to refer to all the different molecular forms in which a protein can be found. Please consult the special issue of Jornal of Proteomics “Towards deciphering proteomes via the proteoform, protein speciation, moonlighting and protein code concepts” published in 2016, vol. 134, pages 1-202). Challenges remain to be solved in order to achieve a compact and automated platform with which to routinely carry out...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venomics; Top-down proteomics; Top-down venomics; Protein species-resolved venomics; Absolute quantification; Inductively coupled plasma mass spectrometry.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100205

Protein profile analysis of Malaysian snake venoms by two-dimensional gel electrophoresis
Provedor de dados: 80
Autores: Vejayan,J; Shin Yee,L; Ponnudurai,G; Ambu,S; Ibrahim,I.
Snake venoms comprise a highly complex mixture of proteins, which requires for their characterization the use of versatile two-dimensional electrophoresis techniques. In the present study, venoms obtained from eight snakes (Ophiophagus hannah, Naja kaouthia, Naja sumatrana, Bungarus fasciatus, Trimeresurus sumatranus, Tropidolaemus wagleri, Enhydrina schistosa and Calloselasma rhodostoma) commonly found in Malaysia were separated based on two independent properties, isoelectric point (pI) and molecular weight (MW). Many differences in snake venoms at the inter-family, inter-subfamily, inter-genus and inter-species levels were revealed. Notably, proteins from individuals of the Viperidae family - Trimeresurus sumatranus, Tropidolaemus wagleri and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Protein profile analysis; Two-dimensional gel electrophoresis; SDS-PAGE.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400013

Management of venomous snakebites in dogs and cats in Brazil
Provedor de dados: 80
Autores: Ferreira Júnior,R.S.; Barravieira,B.
Snake envenoming is a major problem both to veterinary and human medicine in tropical countries due to high incidence, severity, and sequelae. In Brazil, most envenomings involving animals are caused by Bothrops and Crotalus snakes; these are the highest risk to animals. This study reports on Bothrops and Crotalus envenomings in dogs, the main species responsible for epidemiology, pathogenesis, venom action, clinical signs, sequelae and complications, clinical pathology, necropsy findings, diagnosis, and treatment. Veterinarians must be capable of identifying the snake not only by observing its characteristics but also symptom evolution.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snakes; Bothops; Crotalus; Dog.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200002

Comparison between traditional techniques and polymerase chain reaction (PCR) for human tuberculosis diagnosis
Provedor de dados: 80
Autores: Assis,A. C. B.
Tipo: Info:eu-repo/semantics/article
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400018

Scorpionism in Brazil in the years 2000 to 2012
Provedor de dados: 80
Autores: Reckziegel,Guilherme Carneiro; Pinto,Vitor Laerte.
Background Scorpionism is a serious public health problem in Brazil. Nationwide epidemiological analyses of scorpion stings are scarce. In this context, the present study aims to provide an epidemiological analysis of accidents involving scorpions in Brazil.Methods An analytical epidemiological study of the scorpion accidents reported in the Information System for Notifiable Diseases (SINAN) was conducted from 2000 to 2012 in Brazil.Results During this period, 482,616 accidents and 728 deaths were reported. The annual average incidence and mortality rates per 100,000 inhabitants were 19.6 and 0.030, respectively, with annual average lethality rate of 0.16%. The highest accident frequency was recorded in male subjects, aged 20–49 years, from September to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tityus; Scorpion sting; Epidemiological profile; Public health; Brazil.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200337

Cell migration inhibition activity of a non-RGD disintegrin from Crotalus durissus collilineatus venom
Provedor de dados: 80
Autores: Oliveira,Isadora Sousa de; Manzini,Rafaella Varzoni; Ferreira,Isabela Gobbo; Cardoso,Iara Aimê; Bordon,Karla de Castro Figueiredo; Machado,Ana Rita Thomazela; Antunes,Lusânia Maria Greggi; Rosa,José Cesar; Arantes,Eliane Candiani.
Abstract  Background: In recent decades, snake venom disintegrins have received special attention due to their potential use in anticancer therapy. Disintegrins are small and cysteine-rich proteins present in snake venoms and can interact with specific integrins to inhibit their activities in cell-cell and cell-ECM interactions. These molecules, known to inhibit platelet aggregation, are also capable of interacting with certain cancer-related integrins, and may interfere in important processes involved in carcinogenesis. Therefore, disintegrin from Crotalus durissus collilineatus venom was isolated, structurally characterized and evaluated for its toxicity and ability to interfere with cell proliferation and migration in MDA-MB-231, a human breast cancer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durissus collilineatus; Non-RGD disintegrin; Cell migration; Cell adhesion; Human breast cancer; MDA-MB-231.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100319

Allosteric interactions between receptor site 3 and 4 of voltage-gated sodium channels: a novel perspective for the underlying mechanism of scorpion sting-induced pain
Provedor de dados: 80
Autores: Feng,Yi-Jun; Feng,Qi; Tao,Jie; Zhao,Rong; Ji,Yong-Hua.
AbstractBackground BmK I, a site-3-specific modulator of voltage-gated sodium channels (VGSCs), causes pain and hyperalgesia in rats, while BmK IT2, a site-4-specific modulator of VGSCs, suppresses pain-related responses. A stronger pain-related effect has been previously attributed to Buthus martensi Karsch (BmK) venom, which points out the joint pharmacological effect in the crude venom.Methods In order to detect the joint effect of BmK I and BmK IT2 on ND7-23 cells, the membrane current was measured by whole cell recording. BmK I and BmK IT2 were applied successively and jointly, and the synergistic modulations of VGSCs on ND7-23 cells were detected.Results Larger peak I Na and more negative half-activation voltage were elicited by joint application of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BmK I; BmK IT2; Synergistic effect; Allosteric interactions.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100347

Tele-education environment and didactic iconography in sexual transmitted diseases
Provedor de dados: 80
Autores: Ferreira,Ana Silvia S. B. S.
Tipo: Info:eu-repo/semantics/other
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400014

Medical care using telemedicine tools
Provedor de dados: 80
Autores: Ferreira Junior,Rui Seabra; Ferreira,Ana Silvia Sartori Barraviera S.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200001

Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification
Provedor de dados: 80
Autores: Marassá,A. M.; Rosa,M. D. B.; Gomes,A. C.; Consales,C. A.
The knowledge of mosquitoes Culicidae host feeding patterns is basic to understand the roles of different species and to indicate their importance in the epidemiology of arthropod-borne diseases. A laboratory assay was developed aiming at standardizing the biotin-avidin sandwich enzyme-linked immunosorbent assay, which was unprecedented for mosquito blood meal identification. The enzyme-linked immunosorbent assay (ELISA) activity was evaluated by the detection of titers on each sample of the 28 blood-fed Culex quinquefasciatus. In light of the high sensitivity that the technique permits, by means of small quantities of specific antibodies commercially provided and phosphatase substrate which reinforces additional dilutions, human and rat blood meals were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotin/avidin sandwich enzyme-linked immunosorbent assay; Culicidae; Blood meal identification.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200008

An alternative method to isolate protease and phospholipase A2 toxins from snake venoms based on partitioning of aqueous two-phase systems
Provedor de dados: 80
Autores: Gómez,GN; Nerli,BB; Acosta,OC; Picó,GA; Leiva,LCA.
Snake venoms are rich sources of active proteins that have been employed in the diagnosis and treatment of health disorders and antivenom therapy. Developing countries demand fast economical downstream processes for the purification of this biomolecule type without requiring sophisticated equipment. We developed an alternative, simple and easy to scale-up method, able to purify simultaneously protease and phospholipase A2 toxins from Bothrops alternatus venom. It comprises a multiple-step partition procedure with polyethylene-glycol/phosphate aqueous two-phase systems followed by a gel filtration chromatographic step. Two single bands in SDS-polyacrylamide gel electrophoresis and increased proteolytic and phospholipase A2 specific activities evidence the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Partition; Snake toxins; Isolation; Phospholipase A2; Proteases.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300008

Effects of manganese (Mn2+) on neurotoxic and myotoxic activities induced by Crotalus durissus terrificus and Bothrops jararacussu venoms in chick biventer cervicis preparations
Provedor de dados: 80
Autores: Bueno,L. G. F.; Leite,G. B.; Cruz-Höfling,M. A.; Rodrigues-Simioni,L.; Oshima-Franco,Y.
In the present study, manganese (Mn2+), a neuromuscular blocker with pre and postsynaptic actions, was used to verify the neurotoxicity and myotoxicity induced by Crotalus durissus terrificus (Cdt) and Bothrops jararacussu (Bjssu) venoms in biventer cervicis preparations (BCp). Preparations pretreated with 0.66 and 1.6mM Mn2+ did not affect Cdt venom-induced blockage nor change KCl-induced contracture but partially reduced ACh-induced contracture. However, both Mn2+ concentrations partially hindered Bjssu venom-induced blockage after washing the preparations with Krebs solution, and only 1.6mM Mn2+ preparations significantly recovered ACh-induced contracture. The effect of Cdt venom myotoxicity on contractile responses was different from that of Bjssu...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Contracture; Divalent cation; Mn2+; Myotoxicity; Neuromuscular blockage; Neurotoxicity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200007

Lesions caused by Africanized honeybee stings in three cattle in Brazil
Provedor de dados: 80
Autores: Caldas,Saulo Andrade; Graça,Flávio Augusto Soares; de Barros,Júlia Soares Monteiro; Rolim,Márcia Farias; Peixoto,Tiago da Cunha; Peixoto,Paulo Vargas.
We report three cases of stings by Africanized bees in cattle in the state of Rio de Janeiro, Brazil. Erythema, subcutaneous edema, necrosis accompanied by skin detachment, and subsequent skin regeneration were observed, especially on the head and dewlap. Histopathological examinations performed 45 days later revealed complete skin reepithelialization with moderate dermal fibrosis. The clinical picture and differential diagnosis are discussed in the present manuscript, with a focus on photosensitization, which causes cutaneous lesions on the head (sequela) with cicatricial curving of the ears and can be very similar to what is observed in cattle attacked by swarms of bees. The distinction between photosensitization and bee sting lesions can be made with a...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Cattle; Bees; Sting; Poisoning; Accidents.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100502

Snake venom derived fribrin glue as an inducer of tendineous healing in dogs: histopathological and biomechanical study
Provedor de dados: 80
Autores: Ferraro,G. C.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100013

Mechanisms of virus resistance and antiviral activity of snake venoms
Provedor de dados: 80
Autores: Rivero,JVR; de Castro,FOF; Stival,AS; Magalhães,MR; Carmo Filho,JR; Pfrimer,IAH.
Viruses depend on cell metabolism for their own propagation. The need to foster an intimate relationship with the host has resulted in the development of various strategies designed to help virus escape from the defense mechanisms present in the host. Over millions of years, the unremitting battle between pathogens and their hosts has led to changes in evolution of the immune system. Snake venoms are biological resources that have antiviral activity, hence substances of significant pharmacological value. The biodiversity in Brazil with respect to snakes is one of the richest on the planet; nevertheless, studies on the antiviral activity of venom from Brazilian snakes are scarce. The antiviral properties of snake venom appear as new promising therapeutic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Viruses; Antiviral agents.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400005

Effect of propolis extract on guines pig lung mast cell
Provedor de dados: 80
Autores: Orsi,R. O.; Sforcin,J. M.; Funari,S. R. C.; Gomes,J. C.
The direct effect of ethanolic extract of propolis on guinea pig lung cell suspension containing mast cells, as well as its influence on the histamine release induced by antigen (ovoalbumin 10 mug/ml) and ionophore A 23187 (3 muM) were investigated. Propolis ethanolic extract (300 mug/ml) increased the histamine release in guinea pig lung suspension containing mast cells by a cytotoxic effect. Lower concentrations of propolis had no effect on histamine release. Our results demonstrated that propolis (3, 10, 30, and 100 mug/ml) shows no significant effect on the histamine release induced by ionophore and antigen. Based on these results, we suggest that propolis could directly activate mast cells, promoting inflammatory mediators release by cytotoxic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mast cells; Allergy; Histamine; Propolis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100009

In vitro hemolytic activity of Bothrops lanceolatus (fer-de-lance) venom
Provedor de dados: 80
Autores: Martins,LJ; de Araújo,PMF; Bon,C; Hyslop,S; de Araújo,AL.
Bothrops lanceolatus venom contains a variety of enzymatic and biological activities. The present work investigated the hemolytic activity of this venom and its phospholipase A2 (PLA2). Bothrops lanceolatus venom (6.7 µg/mL) caused indirect hemolysis of cow, horse, rat and sheep erythrocytes, with horse erythrocytes being the most sensitive; no direct hemolysis was observed. Hemolysis in sheep erythrocytes was concentration-dependent (5-11.7 µg/mL) and markedly attenuated by heating the venom for 30 minutes at ≥ 40°C and by the PLA2 inhibitor p-bromophenacyl bromide. An acidic PLA2 (5 µg/mL) purified from B. lanceolatus venom also caused hemolysis. This PLA2 showed immunoprecipitin lines with antivenom against B. lanceolatus, which suggests that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops lanceolatus; Hemolytic activity; Phospholipase A2; Snake venom.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300011

Knowledge and attitudes of health care workers from intensive care units regarding nosocomial transmission of influenza: a study on the immediate pre-pandemic period
Provedor de dados: 80
Autores: Fortaleza,CR; Fortaleza,CMCB.
The transmission of influenza in health care settings is a major threat to patients, especially those with severe diseases. The attitude of health care workers (HCWs) may influence the transmission of countless infections. The current study aimed to quantify knowledge and identify attitudes of HCWs involved in intensive care units (ICUs) regarding the risk of nosocomial influenza transmission. A questionnaire was applied through interviews to HCWs who worked in one of the five ICUs from a teaching hospital. Questions about influenza were deliberately dispersed among others that assessed several infectious agents. Forty-two HCWs were interviewed: nine physicians, ten nurses and 23 nursing technicians or auxiliaries. Among the 42 HCWs, 98% were aware of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Influenza; Health care-acquired infections; Health care workers; Intensive care units; Vaccination.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200007

Development and improvement in the snake antivenom production
Provedor de dados: 80
Autores: Morais,V.
Tipo: Info:eu-repo/semantics/other
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200013

Inefficacy of the association N-methyl glucamine and topical miltefosine in the treatment of experimental cutaneous leishmaniasis by Leishmania (Leishmania) amazonensis
Provedor de dados: 80
Autores: Sampaio,R. N. R.; Lucas,I. C.; Takami,H. L.
Pentavalent antimonial (SbV) is the first treatment for cutaneous leishmaniasis (CL). Other drugs present similar side effects and higher cost. Oral miltefosine is effective to treat kala-azar. The aim of the present study was to compare the efficacy of glucamine (SbV) plus topical miltefosine with glucamine in the treatment of CL. Eighty isogenic C57BL/6 mice were inoculated with Leishmania (Leishmania) amazonensis and divided into two groups: one group was treated with SbV associated with miltefosine, and the other group received SbV plus saline solution. Groups were evaluated according to the diameter of the inoculated foot pad, the culture, and the parasite count using the limiting dilution assay. There was not statistical difference. The efficacy of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cutaneous Leishmaniasis; Miltefosine; N-methyl glucamine.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300004

Scholarly communication in the web 2.0 environment: why not?
Provedor de dados: 80
Autores: Soares,Suely de Brito Clemente.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100001

Determination of potency and paraspecific effects of Androctonus crassicauda (Olivier, 1807) antivenom against Mesobuthus gibbosus (Brullé, 1832) venom (Scorpiones: Buthidae)
Provedor de dados: 80
Autores: Ozkan,O.; Adiguzel,S.; Kar,S.; Yakistiran,S.; Cesaretli,Y.; Karaer,K. Z.
Scorpion envenomation remains a real health problem in many countries. In scorpionism cases, it is often recommended that patients be treated with species-specific antivenom. Androctonus crassicauda venom has been used as antigen for antivenom production in Turkey, where this antivenom, called Turkish antivenom, has also been effective in the treatment of envenomation caused by species other than A. crassicauda. The present study aimed at determining the paraspecific effects and potency of the Turkish antivenom against Mesobuthus gibbosus (Brullé, 1832) venom. To assess the venom toxicity and the antivenom efficacy, we determined the Minimum Lethal Dose (MLD) and the Minimum Effective Dose (MED) instead of LD50 and ED50, respectively. Androctonus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion; Antivenom; Androctonus crassicauda; Mesobuthus gibbosus; Paraspecific activity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200008

Purification and antibacterial activities of an L-amino acid oxidase from king cobra (Ophiophagus hannah) venom
Provedor de dados: 80
Autores: Phua,CS; Vejayan,J; Ambu,S; Ponnudurai,G; Gorajana,A.
Some constituents of snake venom have been found to display a variety of biological activities. The antibacterial property of snake venom, in particular, has gathered increasing scientific interest due to antibiotic resistance. In the present study, king cobra venom was screened against three strains of Staphylococcus aureus [including methicillin-resistant Staphylococcus aureus (MRSA)], three other species of gram-positive bacteria and six gram-negative bacteria. King cobra venom was active against all the 12 bacteria tested, and was most effective against Staphylococcus spp. (S. aureus and S. epidermidis). Subsequently, an antibacterial protein from king cobra venom was purified by gel filtration, anion exchange and heparin chromatography. Mass...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-amino acid oxidase; King cobra; Antibacterial activity; Ophiophagus hannah.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200010

The Cuban scorpion Rhopalurus junceus (Scorpiones, Buthidae): component variations in venom samples collected in different geographical areas
Provedor de dados: 80
Autores: Rodríguez-Ravelo,Rodolfo; Coronas,Fredy I V; Zamudio,Fernando Z; González-Morales,Lidia; López,Georgina Espinosa; Urquiola,Ariel Ruiz; Possani,Lourival D.
Backgound The venom of the Cuban scorpion Rhopalurus junceus is poorly study from the point of view of their components at molecular level and the functions associated. The purpose of this article was to conduct a proteomic analysis of venom components from scorpions collected in different geographical areas of the country. Results Venom from the blue scorpion, as it is called, was collected separately from specimens of five distinct Cuban towns (Moa, La Poa, Limonar, El Chote and Farallones) of the Nipe-Sagua-Baracoa mountain massif and fractionated by high performance liquid chromatography (HPLC); the molecular masses of each fraction were ascertained by mass spectrometry analysis. At least 153 different molecular mass components were identified among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cuba; Hyaluronidase; Peptides; Phospholipase; Proteomics; Rhopalurus junceus; Scorpion.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100309

Histopathological evaluation in experimental envenomation of dogs with Crotalus durissus terrificus venom
Provedor de dados: 80
Autores: Sangiorgio,F.; Sakate,M.; Nogueira,R. M. B.; Tostes,R. A.
The present work evaluated histopathological aspects in experimental envenomation of dogs with Crotalus durissus terrificus venom. Twenty-eight mixed breed adult dogs were divided into three groups of seven animals each: Group I - only venom; Group II - venom + 50ml antiophidic serum + fluid therapy; Group III - venom + 50ml antiophidic serum + fluid therapy + urine alkalization. Lyophilized venom of Crotalus durissus terrificus was reconstituted in saline solution and inoculated subcutaneously at the dose of 1mg/kg body weight. Three animals of each group were subjected to euthanasia, and their muscular tissue, brain, spleen, kidneys, heart, lungs, stomach, small and large intestines, and popliteal lymph node fragments were collected for histopathological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Histopathology; Crotalus durissus terrificus; Dogs; Treatment; Intoxication.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100007

Production of recombinant streptolysin-O for diagnostic use
Provedor de dados: 80
Autores: Velázquez,B.
Tipo: Info:eu-repo/semantics/other
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400019

Paederus beetles: the agent of human dermatitis
Provedor de dados: 80
Autores: Nasir,Shabab; Akram,Waseem; Khan,Rashad Rasool; Arshad,Muhammad; Nasir,Iram.
Background Rove beetles of the genus Paederus cause dermatitis when they come in contact with human skin. This condition is prevalent in some tropical and subtropical regions, such as in northern Pakistan, where it was recorded for the first time by US troops. Despite much research from other countries on this subject, few studies, mostly clinical, have been performed in a Pakistani context. A survey was carried out in villages, towns and cities of Punjab province, Pakistan, to explore the rove beetle population dynamics and to develop a model to elucidate the symptoms, preventive measures and treatment strategies for this dermatitis. Methods The prospective observational and patient surveys were performed bimonthly over a period of two years, in different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dermatitis linearis; Irritation; Lesions; Paederus; Rove beetle; Skin dermatitis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100314

Acute pancreatitis and acute renal failure following multiple hornet stings
Provedor de dados: 80
Autores: Sharma,N.; Balamurugeshan,P. K.; Sharma,A.
Hymenoptera is a class of insects that sting in order to subdue their prey. Humans coming into accidental contact with these insects results in stings that may cause from mild local reaction like weal formation around the sting site to severe systemic reactions such as intravascular hemolysis, acute renal failure, pulmonary edema, cerebral edema, and rarely pancreatitis. We report here the clinical course of a patient who developed concurrent acute pancreatitis and pigment-induced acute renal failure after multiple hornet stings.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hymenoptera; Acute renal failure; Rhabdomyolysis; Hemolysis; Pancreatitis; Hornet stings.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200012

Tumor cytotoxicity of leucurolysin-B, a P-III snake venom metalloproteinase from Bothrops leucurus
Provedor de dados: 80
Autores: Gabriel,LM; Sanchez,EF; Silva,SG; Santos,RG.
Although it has been demonstrated that venoms and toxins from some snakes are able to influence the growth of tumor cells, few antitumoral compounds from Bothrops leucurus venom have been characterized. Leucurolysin-B (leuc-B) is a metalloproteinase class P-III isolated from B. leucurus which possesses an ECD-disintegrin domain. Both ECD-disentegrin and RGD-disintegrin are able to bind to cell surface integrins and inhibit their adherence to their natural ligands. In the present study, the potential efficacy and the cytotoxic effects of leuc-B on glioblastoma, breast cancer and melanoma cell lines were analyzed. The effect of leuc-B on cancer cell survival was evaluated and its 50% inhibitory concentration (IC50) was determined. Morphological alterations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Leucurolysin-B; ECD-disintegrin; Tumor; In vitro study.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100004

Cross-neutralization of the coagulant activity of Crotalus durissus terrificus venom from the northeast of Argentina by bivalent bothropic antivenom
Provedor de dados: 80
Autores: Rodríguez,JP; Gay,CC; Fusco,LS; Gauna,MC; Acosta,OC; Leiva,LC.
Cross-neutralization of Crotalus durissus terrificus venom coagulant activity was tested using bivalent horse antivenom against Bothrops alternatus and Bothrops diporus venoms. Our in vitro and in vivo experiments showed that bothropic antivenom neutralizes the thrombin-like activity of crotalic snake venom and this cross-reaction was demonstrated by immunoassays either with whole venom or a purified thrombin-like enzyme. These results suggest common antigenic properties and, consequently, similar molecular structure among venom thrombin-like enzymes. Besides, they provide information that could be further used in the development of new antivenom formulations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thrombin-like enzyme; Snake venom; Antivenoms; Immunological cross-reaction.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100015

Anti-snake venom: use and adverse reaction in a snake bite study clinic in Bangladesh
Provedor de dados: 80
Autores: Amin,MR; Mamun,SMH; Rashid,R; Rahman,M; Ghose,A; Sharmin,S; Rahman,MR; Faiz,MA.
Snakebites can present local or systemic envenomation, while neurotoxicity and respiratory paralysis are the main cause of death. The mainstay of management is anti-snake venom (ASV), which is highly effective, but liable to cause severe adverse reactions including anaphylaxis. The types of adverse reaction to polyvalent anti-snake venom have not been previously studied in Bangladesh. In this prospective observational study carried out between 1999 and 2001, in the Snake Bite Study Clinic of Chittagong Medical College Hospital, 35 neurotoxic-snake-bite patients who had received polyvalent anti-snake venom were included while the ones sensitized to different antitoxins and suffering from atopy were excluded. The common neurotoxic features were ptosis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snakebite; Anti-snake venom; Adverse reaction; Anaphylaxis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400009

Conferences
Provedor de dados: 80
Tipo: Info:eu-repo/semantics/article
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200013

Immunomodulatory activity of Tityus serrulatus scorpion venom on human T lymphocytes
Provedor de dados: 80
Autores: Casella-Martins,Andrea; Ayres,Lorena R; Burin,Sandra M; Morais,Fabiana R; Pereira,Juliana C; Faccioli,Lucia H; Sampaio,Suely V; Arantes,Eliane C; Castro,Fabiola A; Pereira-Crott,Luciana S.
Abstract  Background Tityus serrulatus scorpion venom (TsV) contains toxins that act on K + and Na + channels and account for the venom’s toxic effects. TsV can activate murine peritoneal macrophages, but its effects on human lymphocytes have been poorly investigated. Considering that lymphocytes may play an important role in envenomation, we assessed whether TsV affects the expression of phenotypic (CD3, CD4, and CD8) and activation (CD69, CD25, and HLA-DR) markers, cell proliferation, and cytokine production in peripheral blood mononuclear cells.  Methods  Cytotoxicity of TsV was evaluated via the MTT assay. Cell proliferation, expression of phenotypic and activation markers, and release of cytokines were assessed using flow cytometry, after treatment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunomodulation; Immunophenotyping; T lymphocyte; Cell proliferation; Cytokine; Tityus serrulatus venom.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100350

Hantaviruses as emergent zoonoses
Provedor de dados: 80
Autores: Ullmann,LS; Souza,LC; Langoni,H.
Hantaviruses belong to the Bunyaviridae family, which consists of vector-borne viruses. These viruses can provoke two infection types: hemorrhagic fever with renal syndrome (HFRS) - which occurs in the Old World - and hantavirus cardiopulmonary syndrome (HCPS) - an emergent zoonosis that can be found in many countries of the western hemisphere. Rodents are hantavirus reservoirs and each species seems to host a different virus type. Humans acquire the infection by inhaling contaminated aerosol particles eliminated by infected animals. The factors involved in the emergence of hantavirus infections in the human population include ecological modifications and changes in human activities. The most important risk factor is contact between man and rodents, as a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hantaviruses; Rodents; Control; Public health.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400002

Serological survey for Leishmania sp. infection in wild animals from the municipality of Maringá, Paraná state, Brazil
Provedor de dados: 80
Autores: Voltarelli,EM; Arraes,SMAA; Perles,; Lonardoni,MVC; Teodoro,U; Silveira,TGV.
Leishmania sp. infection was investigated in wild animals from the Ingá Park, in the municipality of Maringá, Paraná state, Brazil, where American cutaneous leishmaniasis (ACL) is an endemic disease. Sixty-five mammals, comprising Didelphis albiventris, Cerdocyon thous, Lycalopex vetulus, Cebus apella, Dasyprocta azarae, Dasypus novemcinctus, Procyon cancrivorus and Nasua nasua, were captured. Blood samples were collected for parasite cultivation. Antibodies were investigated by direct agglutination test (DAT) using Leishmania (Viannia) braziliensis as antigen. Flagellates were observed in blood cultures of 14 (35.9%) Didelphis albiventris. Anti-Leishmania antibodies were detected in 31 (51.6%) specimens of Cerdocyon thous, Lycalopex vetulus, Cebus apella,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Wild animals; Reservoir; Agglutination tests.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400011

Characterization of Clostridium perfringens isolated from mammals and birds from Guwahati city, India
Provedor de dados: 80
Autores: Rahman,Mafruza S; Sharma,RK; Borah,P; Chakraborty,A; Devi,Mandakini RK; Longjam,N.
Of the 102 samples collected from mammals and birds, both domestic and captive wild, 48 were found to be positive for Clostridium perfringens. Most of the mammal isolates (84.38%) appeared to have been collected from clinically affected animals, while 33.33% of the bird samples were from clinically affected and 21.43% from apparently healthy birds infected with C. perfringens. Isolates revealed high sensitivity to ciprofloxacin, enrofloxacin and norfloxacin. Among the isolated C. perfringens, 30 (62.50%) showed DNase production. Hemolytic activity was recorded in 14 (24.16%) of the isolates and 28 (58.33%) showed phospholipase C production. All the phospholipase C positive isolates revealed the presence of cpa gene encoding alpha (α) toxin. Of the 102...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; PCR; Α toxin; Cpa gene; Polymerase chain reaction.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100010

Benznidazole affects expression of Th1, Th17 and Treg cytokines during acute experimental Trypanosoma cruzi infection
Provedor de dados: 80
Autores: Gatto,Mariana; Oliveira,Larissa Ragozo Cardoso; Dias,Fernanda De Nuzzi; Araújo Júnior,João Pessoa; Lima,Carlos Roberto Gonçalves; Lordelo,Eliana Peresi; Santos,Rodrigo Mattos dos; Kurokawa,Cilmery Suemi.
Abstract  Background The present study evaluated the effect of treatment with benznidazole on mRNA expression of IFN-γ, IL-17, IL-10, TGF-β and FoxP3 in spleen and heart tissue of BALB/c mice in the acute phase of an experimental infection with Trypanosoma cruzi, strains JLP or Y.  Methods The mRNA expression of cytokines and parasite load were assessed by q-PCR. Dependent groups were compared using Student's paired t-test and independent groups were compared using Student's unpaired t-test.  Results Infection with the JLP or Y strains increased expression of IFN-γ in the heart and of IL-10 and IL-17 in the spleen and heart compared to uninfected animals. Treatment increased the expression of IFN-γ and decreased the expression of IL-17, IL-10, TGF- β and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chagas disease; Trypanosoma cruzi; Immune response; Cytokines; Q-PCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100320

Comparison between suture and fibrin adhesive derived from snake venom for fixation of connective tissue graft in correction of marginal tissue recession
Provedor de dados: 80
Autores: Chiquito,G.C.M.
Tipo: Info:eu-repo/semantics/article
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200014

Kinetics of venom and antivenom serum levels, clinical evaluation and therapeutic effectiveness in dogs inoculated with Crotalus durissus terrificus venom
Provedor de dados: 80
Autores: Sangiorgio,F.; Sakate,M.; Nogueira,R. M. B.; Araújo JR.,J. P.; Chavez-Olortegui,C.
This work evaluated the clinical and therapeutic aspects as well as serum levels of venom and antivenom IgG by enzyme-linked immunosorbent assay (ELISA) in experimental envenomation of dogs with Crotalus durissus terrificus venom. Twenty-eight mixed breed adult dogs were divided into four groups of seven animals each, Group I: only venom; Group II, venom + 50 ml of anti-bothropic-crotalic serum (50mg) + fluid therapy; Group III, venom + 50 ml of anti-bothropic-crotalic serum + fluid therapy + urine alkalination; Group IV, 50 ml of anti-bothropic-crotalic serum. The lyophilized venom of Crotalus durissus terrificus was reconstituted in saline solution and subcutaneously inoculated at the dose of 1mg/kg body weight. The dogs presented clinical signs of local...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durissus terrificus; Dogs; Clinical signs; ELISA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100008

Histopathological characterization of experimentally induced cutaneous loxoscelism in rabbits inoculated with Loxosceles similis venom
Provedor de dados: 80
Autores: Pereira,NB; Kalapothakis,E; Vasconcelos,AC; Chatzaki,M; Campos,LP; Vieira,FO; Verçosa,BLA; Silva,SS; Ferreira,WM; Moro,L.
Envenomation by Loxosceles bites is characterized by dermonecrotic and/or systemic features that lead to several clinical signs and symptoms called loxoscelism. Dermonecrotic lesions are preceded by thrombosis of the dermal plexus. Recent studies show that atheromatous plaque is prone to thrombosis due to endothelial cell apoptosis. To the best of our knowledge, there are no reports of microscopic dermal lesion and endothelial cell apoptosis induced by Loxosceles similis venom in the literature. Thus, the aim of the present study is to describe histological lesions induced by L. similis venom in rabbit skin and to elucidate whether apoptosis of endothelial cells is involved in the pathogenesis of loxoscelism. Forty male rabbits were split into two groups:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Loxosceles similis; Loxoscelism; Dermonecrosis; Cutaneous loxoscelism; Apoptosis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300005

Key factors of clinical research network capacity building
Provedor de dados: 80
Autores: Li,Guowei; Wu,Qianyu; Jin,Yanling; Vanniyasingam,Thuva; Thabane,Lehana.
Abstract In general, clinical research network capacity building refers to programs aimed at enhancing networks of researchers to conduct clinical research. Although in the literature there is a large body of research on how to develop and build capacity in clinical research networks, the conceptualizations and implementations remain controversial and challenging. Moreover, the experiences learnt from the past accomplishments and failures can assist in the future capacity building efforts to be more practical, effective and efficient. In this paper, we aim to provide an overview of capacity building in clinical research network by (1) identifying the key barriers to clinical research network capacity building, (2) providing insights into how to overcome...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capacity building; Clinical research; Network; Collaboration.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100205

Was the Day Hospital worthwhile?
Provedor de dados: 80
Autores: Meira,DA.
Tipo: Info:eu-repo/semantics/article
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100003

Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice
Provedor de dados: 80
Autores: Marinho,M.; Langoni,H.; Oliveira,S. L.; Lima,V. M. F.; Peiró,J. R.; Perri,S. H. V.; Carreira,R.
Immune response to leptospirosis is mainly humorally mediated, and involves opsonization of leptospires for phagocytosis by macrophages and neutrophils. However, some aspects are still unknown. For a more detailed analysis of the cellular immune response to leptospirosis infection, trials were carried out in order to determine the hydrogen peroxide and nitric oxide (H2O2 and NO) production stimulated or not by Interferon-gamma. The participation of some specific cytokines, such as Tumor Necrosis Factor-alpha (TNF-alpha); Interferon-gamma (IFN-gamma); Interleukin-6 (IL-6); and Interleukin-4 (IL-4), in the immunopathology of this infection was also investigated. For this purpose, we analyzed the supernatant from peritoneal macrophage cell culture and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Cytokines; Cellular immunity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200009

Nutritional, immunological and microbiological profiles of burn patients
Provedor de dados: 80
Autores: Barbosa,ASAA; Calvi,SA; Pereira,PCM.
A burn is a lesion on an organic tissue resultant from direct or indirect action of heat on the organism. The present study aimed to evaluate the nutritional, immunological and microbiological status of burn patients at the Bauru State Hospital, São Paulo state, Brazil, in 2007. Eight patients, aged more than 18 years and injured up to 24 hours, were evaluated at the moment of hospitalization and seven days later. All victims were males with a mean age of 38 years. On average, 17.5% of their body surfaces were burned and 50% of the patients were eutrophic. There were significant alterations in levels of erythrocytes, hemoglobin, hematocrit, total protein and albumin due to increased endothelial permeability, direct destruction of proteins in the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Burn; Nutrition; Infection; TNF-α; IL-6; Laboratory exams.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400014

Identification and characterization of the first endogenous phospholipase A2 inhibitor from a non-venomous tropical snake, Boa constrictor (Serpentes: Boidae)
Provedor de dados: 80
Autores: Fortes-Dias,Consuelo L.; Macedo,Diego Henrique Fagundes; Barbosa,Rafaella Pereira; Souza-Silva,Gabriel; Ortolani,Paula Ladeira.
Abstract  Background:  Endogenous phospholipase A2 inhibitors from snake blood (sbPLIs) have been isolated from several species around the world, with the primary function of self-protection against the action of toxic phospholipases A2. In American snakes, sbPLIs were solely described in pit vipers, in which the natural protection role is justified. In this study, we described a sbPLI in Boa constrictor (popularly known as jiboia), a non-venomous snake species from America.  Methods:  PLA2 inhibitory activity was tested in the blood plasma of B. constrictor using C. d. terrificus venom as the enzyme source. Antibodies developed against CNF, a sbγPLI from Crotalus durissus terrificus, were used to investigate the presence of homologues in the blood plasma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phospholipase A2 inhibitor; Phospholipase A2; Boidae; Snakes; Rattlesnake venom.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100305

Pre-hospital treatment of snake envenomation in patients presented AT a tertiary care hospital in Northwestern India
Provedor de dados: 80
Autores: Chauhan,S.; Faruqi,S.; Bhalla,A.; Sharma,N.; Varma,S.; Bali,J.
Snakebite is an important medical emergency. Anti-snake venom along with supportive care is the only specific treatment. However, many people put their faith in non-registered medical practitioners. Where medical aid is available, lack of trained health personnel jeopardizes the situation. This retrospective study, the first of its kind, was aimed at studying the pre-hospital treatment as well as the behavior of patients bitten by snakes and referred to the Postgraduate Institute of Medical Education and Research, Chandigarh, India. A total of 88 cases that occurred between January 1997 and December 2001 were studied. Seventy patients received treatment prior to admission (the majority was treated by non-registered medical practitioners, registered medical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake envenomation; Clinical features; Treatment; First aid.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300006

Biochemical and biological evaluation of gyroxin isolated from Crotalus durissus terrificus venom
Provedor de dados: 80
Autores: Barros,LC; Soares,AM; Costa,FL; Rodrigues,VM; Fuly,AL; Giglio,JR; Gallacci,M; Thomazini-Santos,IA; Barraviera,SRCS; Barraviera,B; Ferreira Junior,RS.
Gyroxin, a thrombin-like enzyme isolated from Crotalus durissus terrificus venom and capable of converting fibrinogen into fibrin, presents coagulant and neurotoxic activities. The aim of the present study was to evaluate such coagulant and toxic properties. Gyroxin was isolated using only two chromatographic steps - namely gel filtration (Sephadex G-75) and affinity (Benzamidine Sepharose 6B) - resulting in a sample of high purity, as evaluated by RP-HPLC C2/C18 and electrophoretic analysis that showed a molecular mass of 30 kDa. Gyroxin hydrolyzed specific chromogenic substrates, which caused it to be classified as a serine proteinase and thrombin-like enzyme. It was stable from pH 5.5 to 8.5 and inhibited by Mn²+, Cu²+, PMSF and benzamidine. Human...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gyroxin; Neurotoxicity; Coagulant activity; Crotalus durissus terrificus; Serine proteinase.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100004

Evaluation of the effects of photooxidized Echis carinatus venom on learning, memory and stress
Provedor de dados: 80
Autores: Reddy,C. M.; Gawade,S. P.
Snake venoms are a mixture of complex proteins, which have many physical and pharmacological properties. Photochemical detoxification has been suggested to generate photooxidized Echis carinatus venom product (POECVP). Antigenically-active photooxidized species of Echis carinatus venom could be obtained by exposing the venom to ultraviolet radiation (UVR) in the presence of methylene blue. The aim of the present study was to evaluate the effects of POECVP on learning, memory and stress in rats. Detoxification of the photooxidized venom was evident since the POECVP-treated group had longer survival time than the group of mice treated with Echis carinatus venom product (ECVP) following intraperitoneal and intracerebral injections. Photooxidized Echis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Learning and memory; Photooxidized Echis carinatus venom product; T-maze; Forced swim test; Elevated plus-maze.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400010

Molecular detection and characterization of cpb2 gene in Clostridium perfringens isolates from healthy and diseased chickens
Provedor de dados: 80
Autores: Tolooe,A; Shojadoost,B; Peighambari,SM.
Clostridium perfringens is an important pathogen in both human and veterinary medicine. Necrotic enteritis (NE) is the most clinically dramatic bacterial enteric disease of poultry induced by C. perfringens. The pathogenicity of this bacterium is associated with the production of extracellular toxins produced by some of its strains, such as beta2 toxin. The exact role of beta2 toxin in NE pathogenesis is still controversial. In the present study, C. perfringens isolates from healthy and diseased poultry flocks from different parts of Iran were analyzed by PCR assay to determine the presence of all variants of the beta2 toxin gene (cpb2). The products of two positive cpb2 PCR reactions were sequenced, compared to each other and to the cpb2 sequences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Necrotic enteritis; Chicken; Beta2 toxin; Cpb2; Iran.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100008

Pathogenic characteristics of yeasts isolated from vaginal secretion preserved under mineral oil
Provedor de dados: 80
Autores: Severo Gomes,B; Souza Motta,CM; Lima,AN; Porto,ALF.
In order to evaluate the pathogenicity of yeasts isolated from vaginal secretion of pregnant and non-pregnant women - stored in mineral oil at the URM Mycology Collection, Department of Mycology, Federal University of Pernambuco - 30 samples belonging to the genera Candida, Rhodotorula, Trichosporon, and Kloeckera, were studied regarding their pathogenic characteristics, ability to grow at room temperature (28°C ± 1°C), 37°C, and 42°C for 72 hours, and production of both phospholipase and proteinase. Results showed that all 30 isolates (100%) were able to grow at room temperature and 37°C, and that 17 samples (57%) were able to grow at 42°C. Evaluation of enzymatic activity showed protease activity in only two isolates (7%), namely C. maritima and C....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pathogenicity; Yeasts; Enzymes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400013

Nanoparticulate drug delivery systems for the treatment of neglected tropical protozoan diseases
Provedor de dados: 80
Autores: Volpedo,Greta; Costa,Lourena; Ryan,Nathan; Halsey,Gregory; Satoskar,Abhay; Oghumu,Steve.
Abstract Neglected Tropical Diseases (NTDs) comprise of a group of seventeen infectious conditions endemic in many developing countries. Among these diseases are three of protozoan origin, namely leishmaniasis, Chagas disease, and African trypanosomiasis, caused by the parasites Leishmania spp., Trypanosoma cruzi, and Trypanosoma brucei respectively. These diseases have their own unique challenges which are associated with the development of effective prevention and treatment methods. Collectively, these parasitic diseases cause more deaths worldwide than all other NTDs combined. Moreover, many current therapies for these diseases are limited in their efficacy, possessing harmful or potentially fatal side effects at therapeutic doses. It is therefore...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Chagas; Trypanosomiasis; Nanoparticles; Liposomes; Nanotubes; Nanodiscs; Micelles.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100201

Environmental parameters and antimicrobial susceptibility of enterobacteriaceae isolated from estuarine waters of São Vicente, São Paulo state, Brazil
Provedor de dados: 80
Autores: Lourenço,N. G. G. S.; Takahashi,C. K.; Lopes,T. F.; Lopes,C. A. M.
Diseases transmitted by water consists a serious public health problem and enterobacteria are the main group of microorganisms responsible for outbreaks in humans. Such pathogenic bacteria proliferate in water polluted by domestic and industrial sewage and reach the population through seawater contact. The aim of the present work was to study environmental parameters as well as to identify Enterobacteriaceae species and their antimicrobial susceptibility in water samples collected from the estuarine area of São Vicente city (São Paulo State, Brazil). Strains were identified by using traditional biochemical tests described in literature and antimicrobial susceptibility tests were carried out using the disk diffusion method. Out of 26 samples, Escherichia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Estuarine water; Environmental parameters; Enterobacteriaceae; Antimicrobial susceptibility.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200006

Evaluation of the efficacy of vacuum cleaners for the integrated control of brown spider Loxosceles intermedia
Provedor de dados: 80
Autores: Ramires,E. N.; Retzlaff,A. V. L.; Deconto,L. R.; Fontana,J. D.; Marques,F. A.; Marques-da-Silva,E.
Some venomous spiders of the genus Loxosceles can reach high population densities inside and around houses. In Brazil, most spider accidents are related to Loxosceles intermedia. Control of loxoscelism should utilize integrated pest management tools, such as vacuum cleaners, to eliminate egg sacs, webs and spiders. The present study tested the efficacy of one type of vacuum cleaner (for professional and domestic use) in the control of L. intermedia populations. Cockroaches (Pycnoscelus surinamensis) were used in some tests for comparison. Vacuuming using standard accessories or a paper tube resulted in the death of all female (n=60), male (n=60), young (n=60) and just-hatched (n=60) L. intermedia, and all egg sacs (n=5) were destroyed. The removal of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Loxoscelism; Loxosceles intermedia; Vacuum cleaners; Venomous animals; Spiders.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300005

Melioidosis parotitis in children
Provedor de dados: 80
Autores: Dance,David.
Abstract A recent paper published in JVATiTD reporting a child in Hainan with parotitis caused by Burkholderia pseudomallei misleadingly described parotitis as a rare manifestation of melioidosis. In fact, it is one of the commonest forms of paediatric melioidosis seen in other parts of Southeast Asia, although interestingly not in Australia.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Melioidosis Parotitis; B. pseudomallei; Paediatric Children.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100801

Vipoxin specificity studied by gas chromatographic determination of enzymatic reaction products. Influence of Ca2+, Mg2+ and Sr2+
Provedor de dados: 80
Autores: Atanasov,V.; Bardarov,V.; Aleksiev,B.; Mitewa,M.
Gas chromatographic procedure with mass spectrometric detection was applied to quantitatively determine the enzymatic specificity and activity of vipoxin (a neurotoxin from the Vipera ammodytes meridionalis venom) as well as the influence of Ca2+, Mg2+ and Sr2+ on these properties.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vipoxin phospholipase A2; Enzyme specificity Ca2+ Mg2+ Sr2+ influence.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300011

Tissue apoptosis in mice infected with Leptospira interrogans serovar Icterohaemorrhagiae
Provedor de dados: 80
Autores: Marinho,Márcia; Táparo,Cilene Vidovix; Oliveira Júnior,Itamar S.; Perri,Silvia Helena Venturoli; Cardoso,Tereza Cristina.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Caspases; Programmed cell death; Mice; Inflammatory response.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100326

Snakes and snakebite envenoming in Northern Tanzania: a neglected tropical health problem
Provedor de dados: 80
Autores: Kipanyula,M. J.; Kimaro,W. H.
Background Snakebites cause considerable human and livestock injuries as well as deaths worldwide, and particularly have a high impact in sub-Saharan Africa. Generating a basic platform of information on the characteristics of snakes and snakebites in various countries is relevant for designing and implementing public health interventions.Methods This study was performed to identify types of snakes and some of the characteristics of snakebite cases in two communities, an agricultural and a pastoralist, in Arusha region, northern Tanzania. A total of 30 field visits were carried out in areas considered by local inhabitants to be potential microhabitats for snakes. Direct observation of snake types based on morphological features and a structured...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venomous snakes; Snakebites; Antivenom; Meserani; Tanzania.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100339

FabAV antivenin use after copperhead snakebite: clinically indicated or knee-jerk reaction?
Provedor de dados: 80
Autores: Gale,Stephen C.; Peters,Jo Ann; Allen,LaDonna; Creath,Robert; Dombrovskiy,Viktor Y.
Abstract  Background  Crotalidae Polyvalent Immune Fab (Ovine) (FabAV) antivenin is commonly recommended after pit viper snakebites. Because copperhead envenomations are usually self-limited, some physicians are reluctant to use this costly treatment routinely, while others follow a more liberal approach. We hypothesized that, in practice, only patients with evidence of significant (moderate or severe) copperhead envenomation [those with snakebite severity score (SSS) &gt; 3] receive FabAV and examined a large cohort to determine the relationship between clinical findings and FabAV administration.  Methods  All data from patients evaluated for copperhead snakebite at a rural tertiary referral center from 5/2002 to 10/2013 were compiled. Demographics,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copperhead; Venomous snakebite; Antivenin; FabAV; Rural.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100302

Zika virus congenital syndrome: experimental models and clinical aspects
Provedor de dados: 80
Autores: Polonio,Carolina Manganeli; Freitas,Carla Longo de; Zanluqui,Nagela Ghabdan; Peron,Jean Pierre Schatzmann.
Abstract Viral infections have long been the cause of severe diseases to humans, increasing morbidity and mortality rates worldwide, either in rich or poor countries. Yellow fever virus, H1N1 virus, HIV, dengue virus, hepatitis B and C are well known threats to human health, being responsible for many million deaths annually, associated to a huge economic and social cost. In this context, a recently introduced flavivirus in South America, called Zika virus (ZIKV), led the WHO to declare in February 1st 2016 a warning on Public Health Emergency of International Concern (PHEIC). ZIKV is an arbovirus of the Flaviviridae family firstly isolated from sentinels Rhesus sp. monkeys at the Ziika forest in Uganda, Africa, in 1947. Lately, the virus has well adapted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zika virus; Congenital infection; Arthrogryposis; Ocular abnormality; Experimental models.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100207

Biochemical comparison of venoms from young Colombian Crotalus durissus cumanensis and their parents
Provedor de dados: 80
Autores: Céspedes,N; Castro,F; Jiménez,E; Montealegre,L; Castellanos,A; Cañas,CA; Arévalo-Herrera,M; Herrera,S.
Crotalus durissus cumanensis, a rattlesnake endemic to Colombia and Venezuela, is considered one of the most lethal snake species in Latin America. The aim of the present study was to compare the protein content and biological activity of the venom obtained from eight specimens of C. durissus cumanensis, namely two adults from different localities of Colombia and six offspring born in captivity. Protein profiles of crude venoms were analyzed by SDS-PAGE and RP-HPLC, and biological activities were evaluated for lethality, edema, defibrination, hemolytic and coagulant activities to assess individual venoms of adults and a pool of young snake venoms. Transient edema appeared rapidly after venom inoculation, whereas hemorrhagic effect was not observed....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snakes; Crotalus durissus cumanensis; Rattlesnake; Venom.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200009

The construction of knowledge under new paradigms
Provedor de dados: 80
Autores: Barraviera,Benedito; Ferreira Júnior,Rui Seabra.
Tipo: Info:eu-repo/semantics/other
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000100001

Honeybees and caterpillars: epidemiology of accidents involving these animals in the Criciúma region, southern Santa Catarina State, Brazil
Provedor de dados: 80
Autores: Cristiano,MP; Cardoso,DC.
The present study aimed to acquire knowledge regarding some aspects of the epidemiology and injuries provoked by honeybees and caterpillars in southern Santa Catarina State, Brazil. Epidemiological information concerning accidents with both animals was prospectively collected from the System of Injury Notification Information (SINAN) files of the Municipal Health Secretary of the Criciúma region, Brazil. Between 1994 and 2006, 203 cases of accidents were registered, 149 provoked by honeybees (73.39%) and 54 by caterpillars (26.61%). The majority of the victims (200 cases) presented full recovery, two cases presented sequelae after recovery, and only one death was recorded in a case involving honeybee injury. The high frequency of victim recovery suggests...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epidemiology; Honeybees; Caterpillars; Poisonous animals; Accidents; Brazil.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400013

Effects of Androctonus crassicauda (Olivier, 1807) (scorpiones: buthidae) venom on rats: correlation among acetylcholinesterase activities and electrolytes levels
Provedor de dados: 80
Autores: Ozkan,O.; Adiguzel,S.; Kar,S.; Kurt,M.; Yakistiran,S.; Cesaretli,Y.; Orman,M.; Karaer,Z.
Scorpions can be considered living fossils because they have changed so little during the last 400 million years. They are venomous arthropods of the Arachnida class and regarded as relatives of spiders, ticks and mites. The aim of the present study was to evaluate the toxicity of Androctonus crassicauda (Olivier, 1807) venom and its effects on the acetylcholinesterase (AchE) activity and on electrolytes levels in rats. Animals were divided into seven groups of five rats each. Test groups received 250µg/kg of venom solution while control group was treated with 200µl of physiological saline solution (PSS). Blood samples were collected from the animals on the 1st, 2nd 4th, 8th, 12th, and 24th hours after subcutaneous injection of venom. Animals were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion; Androctonus crassicauda; Venom; Acetylcholinesterase; Electrolytes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100005

A comparison between the recombinant expression and chemical synthesis of a short cysteine-rich insecticidal spider peptide
Provedor de dados: 80
Autores: Clement,Herlinda; Flores,Vianey; Diego-Garcia,Elia; Corrales-Garcia,Ligia; Villegas,Elba; Corzo,Gerardo.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecticidal peptides; Protein expression; Bacteria; Theraphosid spider; Chemical synthesis; Cysteine-rich peptides.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100324

Jaburetox: update on a urease-derived peptide
Provedor de dados: 80
Autores: Becker-Ritt,Arlete Beatriz; Portugal,Camila Saretta; Carlini,Célia Regina.
Abstract Urease from Canavalia ensiformis seeds was the first enzyme ever to be crystallized, in 1926. These proteins, found in plants, bacteria and fungi, present different biological properties including catalytic hydrolysis of urea, and also enzyme-independent activities, such as induction of exocytosis, pro-inflammatory effects, neurotoxicity, antifungal and insecticidal properties. Urease is toxic to insects and fungi per se but part of this toxicity relies on an internal peptide (~11 kDa), which is released upon digestion of the protein by insect enzymes. A recombinant form of this peptide, called jaburetox (JBTX), was constructed using jburell gene as a template. The peptide exhibits liposome disruption properties, and insecticidal and fungicidal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peptide; Bacteria; Membranes; Nanoparticles.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100211

Morphological identification of scorpion species from Jazan and Al-Medina Al-Munawara regions, Saudi Arabia
Provedor de dados: 80
Autores: Al-Asmari,A. K.; Al-Saif,A. A.; Abdo,N. M.
The present work is a complementary contribution to the comprehensive study of the scorpion sting syndrome in Saudi Arabia. It deals with the identification and determination of medically important scorpions and the other ones, which were collected from two regions (Jazan and Al-Medina Al-Munawara), based on their morphology (the molecular phylogeny and venom characteristics will appear in subsequent publications). The specimens collected from those two regions were brought to the Research Center laboratories in several batches. Morphological identification of the collected specimens was done employing identification keys. There were 646 specimens collected from Jazan Region. A single species, Nebo hierichonticus (Family Diplocentridae), and five genera...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphology; Parabuthus liosoma; Hottentotta jayakari; Buthidae; Jazan.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400012

Evaluation of prognostic value of cell adhesion molecules in chronic hepatitis C therapy
Provedor de dados: 80
Autores: Abdallah,N; Morsi,M; Hamed,N; Abdel Aziz,H.
The most reliable determination of severity and prognosis in chronic viral hepatitis is the histological staging of the disease, which comprises an invasive procedure and is often not well accepted by patients. The search for alternative non-invasive methods is mandatory especially in follow-ups after initial assessment by biopsy. The aim of this study was to evaluate the role of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in patients under interferon alpha therapy whether responsive or non-responsive to therapy. Thirty chronic hepatitis C patients (CHC) under combined therapy of interferon-α with ribavirin, whether responsive or non-responsive, were included in the study as well as ten healthy controls. Serum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ICAM-1; VCAM-1; Interferon; Ribavirin; Chronic hepatitis C.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300010

Effects of propolis from Brazil and Bulgaria on Salmonella serovars
Provedor de dados: 80
Autores: Orsi,R. O.; Sforcin,J. M.; Funari,S. R. C.; Fernandes-JR.,A.; Rodrigues,P.; Bankova,V.
Propolis shows biological properties such as antibacterial action. This bee product has a complex chemical composition, which depends on the local flora where it is produced. Salmonella serovars are responsible for human diseases that range from localized gastroenteritis to systemic infections. The aim of the present study was to investigate the susceptibility of Salmonella strains, isolated from food and infectious processes, to the antibacterial action of Brazilian and Bulgarian propolis, as well as to determine the behavior of these bacteria, according to the incubation period, in medium plus propolis. Dilution of ethanolic extract of propolis in agar was the used method. Brazilian and Bulgarian propolis showed an antibacterial action against all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propolis; Susceptibility profile; Salmonella; Food; Infection.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400006

Epidemiological characteristics of human rabies in Henan province in China from 2005 to 2013
Provedor de dados: 80
Autores: Li,Guo Wei; Chen,Qiao Ge; Qu,Zhen Yu; Xia,Yao; Lam,Alfred; Zhang,Ding Mei; Lu,Jia Hai.
Background Rabies is very common in People’s Republic of China. Henan province, in the central portion of China, is one of the most densely populated provinces in the entire country. With the new rabies epidemic trend noted in northern and western China, it is necessary to investigate the characteristics of human rabies in this area and control the disease.Methods We chose patients in hospital isolation in 18 municipalities in Henan province as the investigation subjects. Data were collected through systematic reporting from these hospitals, whereas a questionnaire was applied to the relatives of patients.Results A total of 1022 rabies cases were reported. The incidence of human rabies in Henan has increased rapidly since 2005, having peaked in 2007, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human rabies; Zoonosis; Epidemiology; Dog bite.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100340

Scorpionism in Central America, with special reference to the case of Panama
Provedor de dados: 80
Autores: Borges,A; Miranda,RJ; Pascale,JM.
Scorpionism in the Americas occurs mainly in Mexico, northern South America and southeast Brazil. This article reviews the local scorpion fauna, available health statistics, and the literature to assess scorpionism in Central America. Notwithstanding its high toxicity in Mexico, most scorpion sting cases in Guatemala, Belize, El Salvador, Nicaragua, and Costa Rica are produced by species in the genus Centruroides that are only mildly toxic to humans despite the existence of ion channel-active toxins in their venoms. Regional morbidity is low with the exception of Panama, where an incidence of 52 cases per 100,000 inhabitants was recorded for 2007, with 28 deaths from 1998 to 2006. Taxa belonging to the genus Tityus (also present in the Atlantic coast of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpionism; Central America; Tityus; Centruroides; Scorpions.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200002

Zika virus proteins at an atomic scale: how does structural biology help us to understand and develop vaccines and drugs against Zika virus infection?
Provedor de dados: 80
Autores: Valente,Ana Paula; Moraes,Adolfo Henrique.
Abstract In Brazil and in other tropical areas Zika virus infection was directly associated with clinical complications as microcephaly in newborn children whose mothers were infected during pregnancy and the Guillain-Barré syndrome in adults. Recently, research has been focused on developing new vaccines and drug candidates against Zika virus infection since none of those are available. In order to contribute to vaccine and drug development efforts, it becomes important the understanding of the molecular basis of the Zika virus recognition, infection and blockade. To this purpose, it is essential the structural determination of the Zika virus proteins. The genome sequencing of the Zika virus identified ten proteins, being three structural (protein E,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zika virus proteins; Structural biology; Protein structure; Nuclear magnetic resonance spectroscopy; Protein-antibody interaction; Protein-small compound interaction.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100206

Potential envenomation by the aglyphous pseudoxyrhophiine snake Leioheterodon madagascariensis and description of its dentition
Provedor de dados: 80
Autores: Razafimahatratra,Bertrand; Wang,Cynthia; Mori,Akira; Glaw,Frank.
Abstract We report on a case of potential envenomation caused by multiple bites by the aglyphous opisthodont snake Leioheterodon madagascariensis in the left thumb of a healthy adult man, which is among the most serious snakebites hitherto reported from Madagascar. The adult snake (total length &gt; 1 meter) was unusually aggressive before and during capture. The symptoms included extensive bleeding, severe local pain, and substantial swelling of the hand and the distal part of the lower arm. The swelling disappeared entirely after five days, but pain in the thumb (when moved) was recognizable even longer. Although L. madagascariensis is widespread and common in anthropogenic habitats in eastern and western Madagascar, this case report seems to be the...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Madagascar; Lamprophiidae; Pseudoxyrhophiinae; Leioheterodon madagascariensis; Envenomation; Dentition; Microcomputed tomography.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100413

Symptom patterns in adult patients stung by scorpions with emphasis on coagulopathy and hemoglubinuria
Provedor de dados: 80
Autores: Rahmani,AH; Jalali,A.
The aim of this retrospective descriptive study was to highlight clinical manifestations 24-48 hours following referral of adult patients stung by scorpions. This study contains clinical records of 290 patients admitted to Razi Hospital due to scorpion stings in Ahvaz, Khuzestan province from 2004 to 2005. The most prevalent patient age range was 15-20 years (30.3%). The most common sting location (41.3%) was the upper extremity; nearly half (49.6%) had been admitted within 6-24 hours following sting, while a large majority (85.5%) were hospitalized for 24-48 hours. A total of 116 (40%) patients presented hemoglobinuria. Contrary to available prior reports, the symptoms in none of the patients were accompanied by neurological manifestations. Kidney...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Scorpion; Stings; Signs and symptoms; Coagulopathy; Hemoglobinuria.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400011

Efficacy of tannins from Mimosa pudica and tannic acid in neutralizing cobra (Naja kaouthia) venom
Provedor de dados: 80
Autores: Sia,FY; Vejayan,J; Jamuna,A; Ambu,S.
In the present study, the effectiveness of Mimosa pudica tannins (MPT) in neutralizing the lethality of Naja kaouthia venom was compared with commercially derived tannins. Preincubation of MPT with N. kaouthia venom maintained 100% survival of mice after 24 hours. The mouse group in which there was no preincubation, no protection against the effects of the venom was observed. M. pudica tannin was found to be more effective in neutralizing the lethality of N. kaouthia venom when compared to commercial tannic acid. Two protein spots were missing in the two-dimensional gel electrophoresis (2-DE) of the MPT treated mouse indicating the down-regulation of venom proteins. The results from this study indicated that tannins obtained from M. pudica are better than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant extract; Snake venom; Therapy; Tannin; Phospholipase A2.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100006

Errata
Provedor de dados: 80
Autores: Castilhos,P; Pereira,CG; Silva,ALN; Napolitano,DR; Oliveira,F; Souza,MA.
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400019

Behavioral alterations induced by repeated saxitoxin exposure in drinking water
Provedor de dados: 80
Autores: Diehl,Felipe; Ramos,Patricia Baptista; Santos,Juliane Marques dos; Barros,Daniela Martí; Yunes,João Sarkis.
Abstract  Background  Blooms of the saxitoxin-producing cyanobacterium Cylindrospermopsis raciborskii have been contaminating drinking water reservoirs in Brazil for many years. Although acute effects of saxitoxin intoxication are well known, chronic deleterious outcomes caused by repeated saxitoxin exposure still require further investigation. The aim of the present work is to investigate the effects of consumption of drinking water contaminated with C. raciborskii for 30 days on learning and memory processes in rats.  Methods  The effects of saxitoxin (3 or 9 μg/L STX equivalents) or cyanobacteria on behavior was determined using the open field habituation task, elevated plus maze anxiety model task, inhibitory avoidance task, and referential Morris...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saxitoxin; Drinking water; Behavior; Aversive memory; Spatial memory; Rats.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100314

Scorpion sting: a public health problem in El Kelaa des Sraghna (Morocco)
Provedor de dados: 80
Autores: El Oufir,R.; Semlali,I.; Idrissi,M.; Soulaymani,A.; Benlarabi,S.; Khattabi,A.; Ait Moh,M.; Soulaymani Bencheikh,R.
The present study aimed at verifying the impact of a Moroccan strategy against scorpion stings and specifically at identifying the epidemiological features of patients envenomed or just stung by scorpions. It included 11,907 patients from El Kelaa des Sraghna Province, Morocco, who were evaluated over five years (2001-2005). Most stings occurred during the hot period and mainly at night. The average incidence was 3.2 per 1,000 inhabitants; patients &lt;15 years accounted for 34%, and the envenomation rate was 12%. Average lethality rate was 0.7%. Our work evaluated the efficacy of the adopted strategy based on indicators of follow-up, morbidity and lethality due to scorpion sting and envenomation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epidemiology; Scorpions; Strategy; Indicators; El Kelaa; Morocco.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200005

Virulence exaltation of Clostridium perfringens strains from bovines
Provedor de dados: 80
Autores: Baldassi,L.; Barbosa,M.L.; Bach,E.E.; Iaria,S.T.
Ten out of eighty-nine strains biochemically identified as Clostridium perfringens, isolated from bovine organs, were selected by their different results showed in toxigenicity test on mice. Those and the standard strains, ATCC types A, B, C, and D, had their virulence exalted through serial intramuscular inoculation into guinea pigs. Results showed that, for toxigenic strains (6), one or two passages were enough to cause exaltation, while for the atoxigenic (4), five or six inoculations were needed. Esterase electrophoresis of standard and isolated strains, with and without exaltation, was performed. Electrophoresis analysis permits the following conclusions: strains that do not show any clinical symptoms in mice, when exalted demonstrate decreased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Bovine; Electrophoresis; Virulence exaltation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300007

Modulation of macrophage activity, induced by beta-1,3 polyglucose extracted from Saccharomyces cerevisae, in BALB/c mice infected with Toxoplasma gondii
Provedor de dados: 80
Autores: Oliveira,R. A. de.
Tipo: Info:eu-repo/semantics/article
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400025

Toxoplasmosis, leptospirosis and brucellosis in stray dogs housed at the shelter in Umuarama municipality, Paraná, Brazil
Provedor de dados: 80
Autores: Dreer,Márcia Küster de Paula; Gonçalves,Daniela Dib; Caetano,Isabel Cristina da Silva; Gerônimo,Edson; Menegas,Paulo Henrique; Bergo,Danilo; Lopes-Mori,Fabiana Maria Ruiz; Benitez,Aline; Freitas,Julio Cesar de; Evers,Fernanda; Navarro,Italmar Teodorico; Martins,Lisiane de Almeida.
Background : Leptospirosis, toxoplasmosis and brucellosis are diseases with worldwide distribution. Among stray dogs, these zoonoses are facilitated by direct contact with other animal species, by the habit of scavenging garbage and hunting in search of food, drinking standing water, smelling other animals' urine, licking female genitalia and the sexual act itself. The objective of this study was to detect antibodies anti-Toxoplasma gondii, anti-Leptospira spp., anti-Brucella canis and anti-Brucella abortus in stray dogs housed in shelters at Umuarama city, Paraná, Brazil. In order to detect toxoplasmosis, indirect immunofluorescence assay (IFA) was performed, agglutination microscopic (MAT) test for leptospirosis and agar gel immunodiffusion (AGID) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira spp; Brucella abortus; Brucella canis; Toxoplasma gondii; Zoonosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100313

Induction of cell migration and activation in mice by the freshwater sponge Drulia uruguayensis Bonetto &amp;amp; Ezcurra de Drago, 1968 (Porifera: Metaniidae)
Provedor de dados: 80
Autores: Magalhães,AO; Volkmer-Ribeiro,C; Fujimoto,LBM; Barbosa,MF; Cardoso,JL; Barcellos,JFM; Silva,CC; Campos,RR; Cunha,MCF; Freitas-Lemos,AP; Dos-Santos,MC.
Freshwater sponges are abundant in the Amazon region and they have been known to cause dermatitis (acute inflammation) since the beginning of the 20th century. To determine whether additional constituents, besides their body spicules, cause dermatological reactions in humans, an experimental study was developed and carried out using mice and Drulia uruguayensis prepared in three different forms: intact sponges (IS), macerated sponges (MS) or isolated spicules - megascleres (ISM). The cells most commonly involved in inflammatory reactions (mast cells, eosinophils and neutrophils), as well as intraepithelial lymphocytes and degranulated mast cells, were counted so that they could be used as parameters to determine which of the sponge preparations induced the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dermatitis; Amazonia; Cauixi; Freshwater sponges.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100009

In silico analysis of binding interaction of conantokins with NMDA receptors for potential therapeutic use in Alzheimer’s disease
Provedor de dados: 80
Autores: Batool,Maleeha Waqar and Sidra.
Abstract  Background  The N-methyl-D-aspartate (NMDA) receptors are glutamate receptors that play vital roles in central nervous system development and are involved in synaptic plasticity, which is an essential process for learning and memory. The subunit N-methyl D-aspartate receptor subtype 2B (NR2B) is the chief excitatory neurotransmitter receptor in the mammalian brain. Disturbances in the neurotransmission mediated by the NMDA receptor are caused by its overexposure to glutamate neurotransmitter and can be treated by its binding to an antagonist. Among several antagonists, conantokins from cone snails are reported to bind to NMDA receptors.  Methods  This study was designed to analyze the binding mode of conantokins with NMDA receptors in both humans...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: N-methyl-D-aspartate; Glutamate; Synaptic plasticity; NR2B; Neurotransmitter; Antagonists; Conantokins; Docking; In silico; Alzheimer’s dieases.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100317

Enzymatic and biochemical characterization of Bungarus sindanus snake venom acetylcholinesterase
Provedor de dados: 80
Autores: Ahmed,M; Latif,N; Khan,RA; Ahmad,A; Rocha,JBT; Mazzanti,CM; Bagatini,MD; Morsch,VM; Schetinger,MRC.
This study analyses venom from the elapid krait snake Bungarus sindanus, which contains a high level of acetylcholinesterase (AChE) activity. The enzyme showed optimum activity at alkaline pH (8.5) and 45ºC. Krait venom AChE was inhibited by substrate. Inhibition was significantly reduced by using a high ionic strength buffer; low ionic strength buffer (10 mM PO4 pH 7.5) inhibited the enzyme by 1. 5mM AcSCh, while high ionic strength buffer (62 mM PO4 pH 7.5) inhibited it by 1 mM AcSCh. Venom acetylcholinesterase was also found to be thermally stable at 45ºC; it only lost 5% of its activity after incubation at 45ºC for 40 minutes. The Michaelis-Menten constant (Km) for acetylthiocholine iodide hydrolysis was found to be 0.068 mM. Krait venom...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetylcholinesterase; Inhibition; Krait; Ionic strength; Acetylthiocholine iodide; Bungarus sindanus; Snake venom.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200014

Effect of snake venom derived fibrin glue on the tendon healing in dogs: clinical and biomechanical study
Provedor de dados: 80
Autores: Ferraro,G. C.; Moraes,J. R.; Shimano,A. C.; Pereira,G. T.; Moraes,F. R.; Bueno de Camargo,M. H.
The aim of this study was to evaluate the effect of snake venom derived fibrin glue on the strength oftendon healing in dogs. The deep digital flexor tendon of the 5th digit of 24 thoracic limbs was partially sectioned for adhesive application. On the 7th, 15th, and 30th postoperative day tendons segments were removed for the clinical and biomechanical study. Results indicated that 62.5% of the tendons showed stump retraction and 20.8% moderate to excessive adherence, which affected gliding. The biomechanical evaluation showed that, over time, tendon healing gained progressive resistance for maximum traction and permanent deformations with satisfactory results on the 15th day for rigidity and resilience compared to the other two studied periods. Snake...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Fibrin glue; Mechanical properties; Snake venom; Healing; Tendon.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300005

A lung image reconstruction from computed radiography images as a tool to tuberculosis treatment control
Provedor de dados: 80
Autores: Oliveira,Marcela de; Duarte,Sergio Barbosa; Giacomini,Guilherme; Pereira,Paulo Câmara Marques; Souza,Lenice do Rosário de; Miranda,José Ricardo de Arruda; Pina,Diana Rodrigues de.
Abstract  Background: Background: Tuberculosis (TB) is an infectious lung disease with high worldwide incidence that severely compromises the quality of life in affected individuals. Clinical tests are currently employed to monitor pulmonary status and treatment progression. The present study aimed to apply a three-dimensional (3D) reconstruction method based on chest radiography to quantify lung-involvement volume of TB acute-phase patients before and after treatment. In addition, these results were compared with indices from conventional clinical exams to show the coincidence level.  Methods: A 3D lung reconstruction method using patient chest radiography was applied to quantify lung-involvement volume using retrospective examinations of 50 patients who...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Quantification; Lung involvement; Serum tests; Pulmonary function tests.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100302

Detection of bovine Clostridium perfringens by polymerase chain reaction
Provedor de dados: 80
Autores: Piatti,R. M.; Ikuno,A. A.; Baldassi,L.
A polymerase chain reaction (PCR) assay to detect Clostridium perfringens alpha toxin gene (cpa) was used to identify eighty-nine C. perfringens strains obtained from bovine clinical material. The strains were biochemically characterized as C. perfringens. The isolated strains were cultured on plates containing brain heart infusion agar with 5% sheep blood under anaerobic conditions. DNA extraction was performed by boiling. The 324 bp amplification product of cpa was observed in all isolates. C. sordellii, C. botulinum, C. novyi, and C. septicum were also tested but did not produce any alpha toxin gene amplification. These findings suggest that PCR is a useful assay in identifying C. perfringens toxin types.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; PCR; Alpha toxin; Polymerase chain reaction.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200005

A new scenario of bioprospecting of Hymenoptera venoms through proteomic approach
Provedor de dados: 80
Autores: Santos,LD; Pieroni,M; Menegasso,ARS; Pinto,JRAS; Palma,MS.
Tipo: Info:eu-repo/semantics/article
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300004

Risk factors for scorpion stings in the beni mellal province of morocco
Provedor de dados: 80
Autores: Charrab,N; El Oufir,R; Soulaymani,A; Semlali,I; Mokhtari,A; Soulaymani,R.
Scorpion stings comprise a serious problem throughout the globe, especially in regions where they are more frequent. In Morocco, Beni Mellal is one of the provinces most affected by this burden. This study aimed to trace the epidemiological profile of scorpion stings in Beni Mellal in order to improve patient care and prevent both morbidity and mortality. Our work is a retrospective study of scorpion stings based on medical charts of Beni Mellal. From 2002 to 2007, 8,340 cases were registered in this province with an average incidence of 1.36‰. The stings were more frequent in summer months, particularly July and August, and between 6 p.m. and 6 a.m. (59.8%). The average age of victims was 26.54 ± 18.42 years. Children were affected in 30.3% of the cases....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epidemiology; Scorpion; Register; Beni Mellal; Morocco.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400009

Analysis of genetic determinism in mice infected with Trypanosoma cruzi resistant Y strain
Provedor de dados: 80
Autores: Passos,L.A.C.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100014

Bothrops jararacussu venom-induced neuromuscular blockade inhibited by Casearia gossypiosperma Briquet hydroalcoholic extract
Provedor de dados: 80
Autores: Camargo,TM; Nazato,VS; Silva,MG; Cogo,JC; Groppo,FC; Oshima-Franco,Y.
The hydroalcoholic extract of Casearia gossypiosperma Briquet (Flacourtiaceae) was standardized for the first time through quality control procedures including pharmacognostic methods, fingerprint chromatograms, defined amounts of marker substances and physicochemical characteristics. The pharmacological activity of C. gossypiosperma (Cg) hydroalcoholic extract was assayed by a traditional in vitro test, which involved irreversible neuromuscular blockade induced by Bothrops jararacussu (Bjssu) venom (60 µg/mL) in mouse phrenic nerve-diaphragm preparations. Bjssu venom blocked muscle activity for 26 (± 2.0) minutes (n = 6). Cg extract (0.1 mg/mL) induced changes on the baseline muscle activity without impairing the muscle function and inhibited 87.6% (±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibothropic extract; Bothrops jararacussu; Casearia gossypiosperma Briquet; Snake venoms.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300009

Isolated biomolecules of pharmacological interest in hemostasis from Cerastes cerastes venom
Provedor de dados: 80
Autores: Chérifi,Fatah; Laraba-Djebari,Fatima.
Biomolecules from Cerastes cerastes venom have been purified and characterized. Two phospholipases isolated from Cerastes cerastes venom share 51% of homology. CC2-PLA2 exhibits antiplatelet activity that blocks coagulation. CCSV-MPase, a non-hemorrhagic Zn2+-metalloproteinase, significantly reduced the plasmatic fibrinogen level and hydrolyzes only its Bβ chain. Serine proteinases such as RP34, afaâcytin and CC3-SPase hydrolyze the fibrinogen and are respectively α, αβ and αβ fibrinogenases. In deficient human plasma, afaâcytin replaces the missing factors VIII and IX, and activates purified human factor X into factor Xa. It releases serotonin from platelets and directly aggregates human (but not rabbit) blood platelets. RP34 proteinase also had no effect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerastes cerastes venom; Proteinases; Phospholipases A2; Platelets; Blood-clotting; Hemostasis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100201

Are opossums capable of transmitting leptospirosis in urban areas?
Provedor de dados: 80
Autores: Lucheis,SB; Hernandes,GS; Lenharo,DK; Santiago,MEB; Baldini-Peruca,LC.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300002

Anti-leptospirose agglutinins in equine sera, from São Paulo, Goias, and Mato Grosso do Sul, Brazil, 1996-2001
Provedor de dados: 80
Autores: Langoni,H.; Da Silva,A.V.; Pezerico,S.B.; De Lima,V.Y.
Equine leptospirosis can present a non-symptomatic form, an acute clinical form, or even develop chronically, causing reproductive alterations, such as abortion and recurrent uveitis. Since the prevalence of leptospirosis in several countries and regions is widely reported, the objective of this study was to verify the prevailing equine leptospirosis in different regions of Brazil. Sera from 1402 blood samples from horses of different age, sex, breed, and purpose were examined. These samples came from southeastern and central west states of Brazil. The method utilized was the Microscopic Agglutination Test (MAT), with 12 different Leptospira serovars. From the sera tested, 754 (54%) were positive for one (385) or more (372) serovars. These results were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Horse; Serology.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300003

Development of a sensitive enzyme immunoassay (ELISA) for specific identification of Lachesis acrochorda venom
Provedor de dados: 80
Autores: Núñez Rangel,V; Fernández Culma,M; Rey-Suárez,P; Pereañez,JA.
The snake genus Lachesis provokes 2 to 3% of snakebites in Colombia every year. Two Lachesis species, L. acrochorda and L. muta, share habitats with snakes from another genus, namely Bothrops asper and B. atrox. Lachesis venom causes systemic and local effects such as swelling, hemorrhaging, myonecrosis, hemostatic disorders and nephrotoxic symptoms similar to those induced by Bothrops, Portidium and Bothriechis bites. Bothrops antivenoms neutralize a variety of Lachesis venom toxins. However, these products are unable to avoid coagulation problems provoked by Lachesis snakebites. Thus, it is important to ascertain whether the envenomation was caused by a Bothrops or Lachesis snake. The present study found enzyme linked immunosorbent assay (ELISA)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Lachesis; Lachesis acrochorda; Enzyme-linked immunosorbent assay.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200007

Functional and structural characterization of phospholipases A² isolated from Bothrops asper snake venom in Panamá
Provedor de dados: 80
Autores: Quintero,A; Soares,AM.
Tipo: Info:eu-repo/semantics/review
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400019

Rabies review: immunopathology, clinical aspects and treatment
Provedor de dados: 80
Autores: Consales,C. A.; Bolzan,V. L.
Among the diseases of viral origin, rabies is unique in its distribution and range of victims since it can afflict all warm-blooded animals. The interaction between the virus and the host population has facilitated the survival of the disease. The rabies virus (RV) has not changed in any significant way and has been capable of taking advantage of conditions suited to the continuance of rabies. Infection by RV is invariably lethal in the absence of protective immune response which, however, can contribute to the pathogenesis of rabies. Proinflammatory cytokines might affect, directly or indirectly, the levels of neurotrophins, growth factors, neurotransmitters and neurotoxins in the brain by activating glia, neurons, and vascular and immune cells. Although...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Immunopathology; Clinical aspects; Treatment.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100002

Organ tropism during the acute and chronic phases of Trypanosoma cruzi infection in BALB/c mice
Provedor de dados: 80
Autores: Oliveira,LRC; Picka,MCM; Nicolete,VC; Calvi,SA; Marcondes-Machado,J.
The aim of the present study was to investigate the presence of Trypanosoma cruzi in the heart, liver, lung, and kidneys, using hemoculture and PCR analysis, of mice infected with different parasite strains during the acute and chronic phases of infection. Parasitemia curves revealed strain-specific biological behaviors. For the Y and JLP strains, the acute phase of infection started at days six and ten post-infection, parasitemia peaked at days seven and 15 post-infection, the chronic phase started at days nine and 28 post-infection, and animals started dying at days 19 and 120 post-infection, respectively. When the two strains were compared, the JLP strain exhibited reduced and slower replication rates associated with a delayed peak of parasitism and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; PCR; Hemoculture; Organs; Distribution.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100005

Immune reconstitution in HIV-1 infected patients treated for two years with highly active antiretroviral therapy
Provedor de dados: 80
Autores: Almeida,R. A. M. B.; Souza,L. R.; Calvi,S. A.; Ikoma,M. R. V.; Silva,V. A.; Curi,P. R.; Meira,D. A.
The aim of this paper was to evaluate the immune reconstitution of HIV-1 patients subjected to highly active antiretroviral therapy (HAART) for two years or more according to CD45RA and CD45RO cell count; determination of IL-2, IFN-gamma, IL-4, IL-10 and TNF-alpha serum levels; CD4+ T and CD8+ T lymphocyte count; and plasma viral load (VL) determination. For this purpose, a cross sectional study was carried out in the Tropical Diseases Area, Botucatu School of Medicine, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil. Between June 2001 and April 2002, 37 HIV-1 infected patients were evaluated, 13 with treatment indication but untreated (G1), 9 subjected to HAART for 5-7 months (G2), and 15 treated for two years or more (G3); both treated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; Immune reconstitution; Antiretroviral; HAART; Cytokines; CD45RA; CD45RO.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100008

Antiophidian properties of plant extracts against Lachesis muta venom
Provedor de dados: 80
Autores: De Paula,RC; Sanchez,EF; Costa,TR; Martins,CHG; Pereira,PS; Lourenço,MV; Soares,AM; Fuly,AL.
Snakebites comprise a serious health problem in several countries due to their global incidence, which exceeds 2.5 million per year, and the elevated number of victim fatalities. To counteract envenomations, antivenoms have been used regularly for more than a century. Apart from side effects including anaphylactic reactions, antivenoms are not able to efficiently neutralize local tissue damage, which contributes to increasing the severity and morbidity observed in patients. This fact, in turn, may be responsible for economic hardship, particularly in rural populations of developing countries. In the present work, we evaluated the antiophidian properties of 12 Brazilian plant extracts against the hemolytic, coagulant, hemorrhagic and proteolytic effects of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Lachesis muta; Plant extract; Antivenom; Biological activities.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200012

Cloning and purification of the first termicin-like peptide from the cockroach Eupolyphaga sinensis
Provedor de dados: 80
Autores: Liu,Zichao; Yuan,Kehua; Zhang,Ruopeng; Ren,Xuchen; Liu,Xiaolong; Zhao,Shuhua; Wang,Dingkang.
Abstract  Background  Termicin is an antimicrobial peptide with six cysteines forming three disulfide bridges that was firstly isolated from the salivary glands and hemocytes of the termite Pseudacanthotermes spiniger. In contrast to many broad-spectrum antimicrobial peptides, termicin is most active against filamentous fungi. Although more than one hundred complementary DNAs (cDNAs) encoding termicin-like peptides have been reported to date, all these termicin-like peptides were obtained from Isoptera insects.  Methods  The cDNA was cloned by combination of cDNA library construction kit and DNA sequencing. The polypeptide was purified by gel filtration and reversed-phase high performance liquid chromatography (RP-HPLC). Its amino acid sequence was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eupolyphaga sinensis; Cockroach; Termicin-like peptide; Es-termicin; Antifungal peptide.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100303

Non-association between anti-Toxoplasma gondii antibodies and ABO blood group system
Provedor de dados: 80
Autores: Rodrigues,ACF; Uezato,S; Vono,MB; Pandossio,T; Spegiorin,LCJF; Oliani,AH; Vaz Oliani,DCM; Brandão de Mattos,CC; de Mattos,LC.
Toxoplasma gondii infects humans through the gastrointestinal tract (GIT), which elicits humoral immune response with specific antibodies. The expression of the ABO blood group glycoconjugates also occurs in this same system and may influence the human susceptibility of infection by T. gondii. The aim of the present study was to investigate the association between ABO blood group phenotypes and the presence of anti-T. gondii antibodies. Data - including age, results of serology tests for T. gondii infection and ABO blood group phenotypes - were assembled from the medical records of 1,006 pregnant women attended in the Base Hospital of the Medical School of São José do Rio Preto, Brazil, between 2001 and 2004. The chi-square test was used to compare the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human blood groups; Toxoplasma gondii; Toxoplasmosis; ABO blood group; Pregnant women.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200009

BjussuLAAO-II induces cytotoxicity and alters DNA methylation of cell-cycle genes in monocultured/co-cultured HepG2 cells
Provedor de dados: 80
Autores: Machado,Ana Rita Thomazela; Aissa,Alexandre Ferro; Ribeiro,Diego Luis; Ferreira Jr.,Rui Seabra; Sampaio,Suely Vilela; Antunes,Lusânia Maria Greggi.
Abstract  Background: The use of animal venoms and their toxins as material sources for biotechnological applications has received much attention from the pharmaceutical industry. L-amino acid oxidases from snake venoms (SV-LAAOs) have demonstrated innumerous biological effects and pharmacological potential against different cancer types. Hepatocellular carcinoma has increased worldwide, and the aberrant DNA methylation of liver cells is a common mechanism to promote hepatic tumorigenesis. Moreover, tumor microenvironment plays a major role in neoplastic transformation. To elucidate the molecular mechanisms responsible for the cytotoxic effects of SV-LAAO in human cancer cells, this study aimed to evaluate the cytotoxicity and the alterations in DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Epigenetics; GADD45A; CCND1; CDKN1A.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100305

Hypertrophy of NADH-diaphorase positive myenteric neurons in rat jejunum after acute infection caused by Toxoplasma gondii
Provedor de dados: 80
Autores: Pereira,LS; Silva,AV; Araújo,EJA; Sant'Ana,DMG.
Toxoplasmosis, a globally distributed feline-associated zoonosis caused by the protozoan Toxoplasma gondii, affects birds and mammals, including humans. This study assesses the consequences of acute T. gondii infection for NADH-diaphorase positive myenteric neurons in rat jejunum. Ten male Wistar rats (Rattus norvegicus) were divided into two groups: G1 (n = 5) and G2 (n = 5). Animals from G2 were orally inoculated with 500 genotype III (M7741) T. gondii oocysts. Twenty-four hours after inoculation, the animals were euthanized and had their jejuna removed, through laparotomy, and measured (length and width) to calculate their areas. Intestinal segments were submitted to NADH-diaphorase histochemistry to evidence the most metabolically active subpopulation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Jejunum; Myenteric plexus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200011

Proteome of fraction from Tityus serrulatus venom reveals new enzymes and toxins
Provedor de dados: 80
Autores: Amorim,Fernanda Gobbi; Longhim,Heloisa Tavoni; Cologna,Camila Takeno; Degueldre,Michel; Pauw,Edwin De; Quinton,Loïc; Arantes,Eliane Candiani.
Abstract  Background: Tityus serrulatus venom (Ts venom) is a complex mixture of several compounds with biotechnological and therapeutical potentials, which highlights the importance of the identification and characterization of these components. Although a considerable number of studies have been dedicated to the characterization of this complex cocktail, there is still a limitation of knowledge concerning its venom composition. Most of Ts venom studies aim to isolate and characterize their neurotoxins, which are small, basic proteins and are eluted with high buffer concentrations on cation exchange chromatography. The first and largest fraction from carboxymethyl cellulose-52 (CMC-52) chromatography of Ts venom, named fraction I (Fr I), is a mixture of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tityus serrulatus; Scorpion venom; Enzymes; Proteases; ACE inhibitors; Proteome.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100308

In vitro comparative susceptibility to ciprofloxacin and vancomycin in Enterococcus faecalis strains isolated from infection
Provedor de dados: 80
Autores: Genaro,A.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100012

Agaricus brasiliensis polysaccharides stimulate human monocytes to capture Candida albicans, express toll-like receptors 2 and 4, and produce pro-inflammatory cytokines
Provedor de dados: 80
Autores: Martins,Priscila Raquel; Soares,Ângela Maria Victoriano de Campos; Domeneghini,Andrea Vanessa da Silva Pinto; Golim,Márjorie Assis; Kaneno,Ramon.
Abstract  Background Agaricus brasiliensis is a medicinal mushroom with immunomodulatory and antitumor activities attributed to the β-glucans presented in the polysaccharide fraction of its fruiting body. Since β-glucans enhance cellular immunoresponsiveness, in this study we aimed to evaluate the effect of an acid-treated polysaccharide-rich fraction (ATF) of A. brasiliensis on the ability of human monocytes to adhere/phagocyte C. albicans yeast cells, their expression of pattern recognition receptors and their ability to produce cytokines.  Methods  Adhesion/phagocytosis of FITC-labeled C. albicans was evaluated by flow cytometry. Cells were incubated with specific fluorochrome-labeled antibodies for TLR2 and 4, βGR and MR and also evaluated by flow...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agaricus blazei; Agaricus brasiliensis; Monocytes; Pattern recognition receptors (PPR); Toll-like receptors.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100306

Prevalence and epidemiology of chronic hepatitis C among prisoners of Mato Grosso do Sul State, Brazil
Provedor de dados: 80
Autores: Pompilio,MA; Pontes,ERJC; Castro,ARCM; Andrade,SMO; Stief,ACF; Martins,RMB; Mousquer,GJ; Murat,PG; Francisco,RBL; Pompilio,SAL; Rezende,GR; Elias-Junior,E.
In Mato Grosso do Sul state, Brazil, the number of prisoners has increased in the recent years and the control of hepatitis C virus (HCV) has become more complex. The aim of the present study was to estimate the prevalence and identify the genotypes of HCV in prisoners as well as the factors associated with this infectious disease. Thereby, 443 men and 243 women from prisons were interviewed and subjected to blood collection. Anti-HCV reactive samples were analyzed by RT-PCR and genotyped. The overall seroprevalence of HCV infection was 4.8% (95%CI: 3.4 to 6.8%). Furthermore, the prevalence was higher in: men, injecting drug users, tattooed persons, those who were more than 50 years old, individuals who have been arrested multiple times, people with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C; Genotype; Polymerase chain reaction; Epidemiology; Prisoners.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200013

Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine
Provedor de dados: 80
Autores: Teixeira-Araújo,Ricardo; Castanheira,Patrícia; Brazil-Más,Leonora; Pontes,Francisco; Araújo,Moema Leitão de; Alves,Maria Lucia Machado; Zingali,Russolina Benedeta; Correa-Netto,Carlos.
Abstract  Background  Snakebite treatment requires administration of an appropriate antivenom that should contain antibodies capable of neutralizing the venom. To achieve this goal, antivenom production must start from a suitable immunization protocol and proper venom mixtures. In Brazil, antivenom against South American rattlesnake (Crotalus durissus terrificus) bites is produced by public institutions based on the guidelines defined by the regulatory agency of the Brazilian Ministry of Health, ANVISA. However, each institution uses its own mixture of rattlesnake venom antigens. Previous works have shown that crotamine, a toxin found in Crolatus durissus venom, shows marked individual and populational variation. In addition, serum produced from...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Antivenom production; Antivenomics; Crotalus durissus; Crotamine; Crotoxin; Geographic venom variation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100602

Protein identification from the parotoid macrogland secretion of Duttaphrynus melanostictus
Provedor de dados: 80
Autores: Mariano,Douglas Oscar Ceolin; Messias,Marcela Di Giacomo; Spencer,Patrick Jack; Pimenta,Daniel Carvalho.
ABSTRACT  Background:  Bufonid parotoid macrogland secretion contains several low molecular mass molecules, such as alkaloids and steroids. Nevertheless, its protein content is poorly understood. Herein, we applied a sample preparation methodology that allows the analysis of viscous matrices in order to examine its proteins.  Methods: Duttaphrynus melanostictus parotoid macrogland secretion was submitted to ion-exchange batch sample preparation, yielding two fractions: salt-displaced fraction and acid-displaced fraction. Each sample was then fractionated by anionic-exchange chromatography, followed by in-solution proteomic analysis.  Results:  Forty-two proteins could be identified, such as acyl-CoA-binding protein, alcohol dehydrogenase, calmodulin,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amphibian skin secretion; Bufonidae; Duttaphrynus melanostictus; Proteomics; Batch chromatography; Asian common toad.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100318

Identification of a pore-forming protein from sea anemone Anthopleura dowii Verrill (1869) venom by mass spectrometry
Provedor de dados: 80
Autores: Ramírez-Carreto,Santos; Pérez-García,Erick I.; Salazar-García,Sandra I.; Bernáldez-Sarabia,Johanna; Licea-Navarro,Alexei; Rudiño-Piñera,Enrique; Pérez-Martínez,Leonor; Pedraza-Alva,Gustavo; Rodríguez-Almazán,Claudia.
Abstract  Background: Pore-forming proteins (PFP) are a class of toxins abundant in the venom of sea anemones. Owing to their ability to recognize and permeabilize cell membranes, pore-forming proteins have medical potential in cancer therapy or as biosensors. In the present study, we showed the partial purification and sequencing of a pore-forming protein from Anthopleura dowii Verrill (1869). 17.  Methods: Cytolytic activity of A. dowii Verrill (1869) venom was determined via hemolysis assay in the erythrocytes of four mammals (sheep, goat, human and rabbit). The cytotoxic activity was analyzed in the human adherent lung carcinoma epithelial cells (A549) by the cytosolic lactate dehydrogenase (LDH) assay, and trypan blue staining. The venom was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anthopleura; Pore-forming protein; Venom; Lung carcinoma; Sea anemone.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100301

Neuromuscular activity of Bothrops neuwiedi pauloensis snake venom in mouse nerve-muscle preparations
Provedor de dados: 80
Autores: Durigon,A. M.; Borja-Oliveira,C. R.; Dal Belo,C. A.; Oshima-Franco,Y.; Cogo,J. C.; Lapa,A. J.; Souccar,C.; Rodrigues-Simioni,L.
The pharmacological effects of Bothrops neuwiedi pauloensis venom on mouse phrenic nerve-diaphragm (PND) preparations were studied. Venom (20 mug/ml) irreversibly inhibited indirectly evoked twitches in PND preparations (60 ± 10% inhibition, mean ± SEM; p&lt;0.05; n=6). At 50 mug/ml, the venom blocked indirectly and directly (curarized preparations) evoked twitches in mouse hemidiaphragms. In the absence of Ca2+, venom (50 mug/ml), produced partial blockade only after an 80 min incubation, which reached 40.3 ± 7.8% (p&lt;0.05; n=3) after 120 min. Venom (20 mug/ml) increased (25 ± 2%, p&lt; 0.05) the frequency of giant miniature end-plate potentials in 9 of 10 end-plates after 30 min and the number of miniature end-plate potentials which was maximum (562 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Giant MEPPs; Myotoxicity; Neurotoxicity; Presynaptic action.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100004

Envenomation caused by Latrodectus geometricus in São Paulo state, Brazil: a case report
Provedor de dados: 80
Autores: Almeida,Ramb; Ferreira Junior,RS; Chaves,CR; Barraviera,B.
Widow spiders (Latrodectus spp.), also known as "black widows", have a worldwide distribution and can cause latrodectism. To the best of our knowledge, in Brazil, only one case of Latrodectus geometricus (Koch, 1841) envenomation in a human has been reported. The aim of the present report is to describe a spider bite caused by Latrodectus geometricus in a patient who lives in Paranapanema, São Paulo state, Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Latrodectus geometricus; Envenomation; Latrodectism; Brown widow.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300016

Preparation of monoclonal antibodies against gamma-type phospholipase A2 inhibitors and immunodetection of these proteins in snake blood
Provedor de dados: 80
Autores: Li,Jingjing; Xiong,Ying; Sun,Shimin; Yu,Lehan; Huang,Chunhong.
Abstract  Background: The gamma-type phospholipase A2 inhibitor (ΡLIγ) is a natural protein commonly found in snake serum, which can neutralize pathophysiological effects of snake venom phospholipases A2. Therefore, this protein is a potential candidate to the development of a novel antivenom. To the best of our knowledge, there is no antibody currently available for PLIγ identification and characterization.  Methods: Bioinformatics prediction of epitope using DNAStar software was performed based on the sequence of Sinonatrix annularis PLIγ (SaPLIγ). The best epitope 151CPVLRLSNRTHEANRNDLIKVA172 was chosen and synthesized, and then conjugated to keyhole limpet hemocyanin and bovine serum albumin for use as an immunogen and plate-coating antigen,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monoclonal antibody; Phospholipase A2 inhibitor; Epitope prediction.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100314

How to overcome the gap between basic research and clinical trials?
Provedor de dados: 80
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400001

Evaluation of macrophage activity and antibody production in genetically selected mice infected with Leptospira serovar Pomona
Provedor de dados: 80
Autores: Haanwinckel,M. C. S.
Tipo: Info:eu-repo/semantics/other
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100018

Intestinal microbiota and HIV-1 infection
Provedor de dados: 80
Autores: Trindade,E. B. S. M.; Souza,L. R.; Lopes,C. A. M.; Pereira,P. C. M.
The intestinal microbiota consists of a qualitatively and quantitatively diverse range of microorganisms dynamically interacting with the host. It is remarkably stable with regard to the presence of microorganisms and their roles which, however, can be altered due to pathological conditions, diet composition, gastrointestinal disturbances and/or drug ingestion. The present review aimed at contributing to the discussion about changes in the intestinal microbiota due to HIV-1 infection, focusing on the triad infection-microbiota-nutrition as factors that promote intestinal bacterial imbalance. Intestinal microbiota alterations can be due to the HIV-1 infection as a primary factor or the pharmacotherapy employed, or they can be one of the consequences of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal microbiota; Nutrition; Infection; HIV-1.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200003

Pharmacological characterization of venoms obtained from Mexican toxoglossate gastropods on isolated guinea pig ileum
Provedor de dados: 80
Autores: Rojas,A; Feregrino,A; Ibarra-Alvarado,C; Aguilar,MB; Falcón,A; Heimer de la Cotera,E.
The protein-containing extracts prepared from the venom ducts of Conus austini, Conus spurius and Polystira albida caused a concentration-dependent inhibition of spontaneous contractions in guinea pig ileum. The most potent extract was obtained from P. albida venom ducts (IC50 = 0.11 ± 0.02 µg protein/mL). The three extracts produced a moderate inhibition of contractions elicited by acetylcholine (ACh 1 µM), suggesting the presence of anticholinergic compounds. The contractile response elicited by nicotine (10 µM) was significantly reduced by the extracts prepared from the ducts of C. austini and P. albida, which indicates that the venom produced by these species contains toxins that target neuronal nicotinic receptors. All three extracts significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conoidea; Conus austini; Conus spurius; Guinea pig ileum; Polystira albida; Turridae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300009

Scorpions and life-history strategies: from evolutionary dynamics toward the scorpionism problem
Provedor de dados: 80
Autores: Lourenço,Wilson R.
Abstract This work aims to contribute to the general information on scorpion reproductive patterns in general including species that can be noxious to humans. Scorpions are unusual among terrestrial arthropods in several of their life-history traits since in many aspects their reproductive strategies are more similar to those of superior vertebrates than to those of arthropods in general. This communication focuses mainly on the aspects concerning embryonic and post-embryonic developments since these are quite peculiar in scorpions and can be directly connected to the scorpionism problem. As in previous similar contributions, the content of this communication is addressed mainly to non-specialists whose research embraces scorpions in several fields such as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion; Reproductive strategies; Embryonic; Postembryonic development.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100206

Protective effects of the antioxidant Ginkgo biloba extract and the protease inhibitor aprotinin against Leiurus quinquestriatus venom-induced tissue damage in rats
Provedor de dados: 80
Autores: Fatani,A. J.; Al-Zuhair,H. A.; Yaquob,H. I.; Abdel-Fattah,A. A.; El-Sayed,M. I.; El-Sayed,F. A.
Oxidative stress and proteases have been implicated in several diseases and extensive evidence indicates that antioxidants and protease inhibitors help prevent organ functional damage. Leiurus quinquestriatus (LQQ) scorpion venom causes cellular injuries that may lead to multiple organ failure. Thus, the capability of the antioxidant "natural standardized extract of Gingko biloba leaves (Gin, EGb 761)" and the non-selective protease inhibitor, aprotinin, in ameliorating venom-induced biochemical alterations indicative of cellular injury and oxidative stress was studied to determine their effectiveness in protecting rats from venom-evoked cellular damages. Thus, in this study, rats were treated with LQQ venom (0.3mg.kg-1, subcutaneously) alone or after Gin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leiurusquinquestriatus; Ginkgo; Aprotinin; Scorpion venoms.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200008

The 6th international conference on envenomation by Snakebites and Scorpion Stings in Africa: a crucial step for the management of envenomation
Provedor de dados: 80
Autores: Chippaux,Jean-Philippe; Akaffou,Marc Hermann; Allali,Bernard Kouadio; Dosso,Mireille; Massougbodji,Achille; Barraviera,Benedito.
Abstract During the 6th International Conference on Envenomation by Snakebites and Scorpion Stings in Africa held in Abidjan, from 1 to 5 June 2015, the measures for the management of envenomation were discussed and new recommendations were adopted by the participants. The high incidence and severity of this affliction were confirmed by several studies conducted in African countries. The poor availability of antivenom, particularly because of the cost, was also highlighted. Some experiences have been reported, mainly those regarding the financial support of antivenom in Burkina Faso (more than 90 %) and Togo (up to 60 %) or the mandatory reporting of cases in Cameroon. Key recommendations concerned: improvement of epidemiological information based on case...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Envenomation; Antivenom; Epidemiology; Drug policy; Africa.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100101

Enterovirus infections and type 1 diabetes mellitus: is there any relationship?
Provedor de dados: 80
Autores: Tavares,RG; Trevisol,RB; Comerlato,J; Dalzochio,T; Feksa,LR; Spilki,FR; Berlese,DB.
Several health organizations have classified diabetes mellitus, a metabolic syndrome, as the epidemic of the century, since it affects millions of people worldwide and is one of the top ten causes of death. Type 1 diabetes is considered to be an autoimmune disease, in which autoaggressive T cells infiltrate the islets of Langerhans in the pancreas, leading to the destruction of insulin producing beta cells. The risk of the disease is modulated by genetic factors, mainly genes coding for human leukocyte antigens (HLA). However, the incidence of this disease has increased significantly during the recent decades, which cannot be explained only by genetic factors. Environmental perturbations have also been associated to the development of diabetes. Among these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus type I; Enterovirus; Autoimmune disease.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100002

A historical approach to scorpion studies with special reference to the 20 th and 21st centuries
Provedor de dados: 80
Autores: Lourenço,Wilson R.
This work provides historical context about scorpion studies from the end of the 19 th century to the present day. The content is mainly addressed to non-zoologists, working in research fields that embrace scorpion biology, notably to those working with venoms and toxins. The historical aspects described include academic professional scholars who worked on scorpion classification and general distribution patterns; and to a lesser extent, on studies of ecology and natural history. The aim is not to provide an exhaustive description of all scholars who in one way or another became involved with scorpions, but rather of those who greatly contributed during a given period to the research of these organisms. No critical analysis of the work of previous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion studies; Historical approach; 20th century; 21st century; Taxonomy; Biology.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200204

Origin, transfer and distribution of cantharidin-related compounds in the blister beetle Hycleus scabiosae
Provedor de dados: 80
Autores: Nikbakhtzadeh,MR; Vahedi,M; Vatandoost,H; Mehdinia,A.
Cantharidin provides chemical protection for the coleopteran families Meloidae and Oedemeridae. In the present study, it was observed that cantharidin concentration in Hycleus scabiosae was slightly decreased from mated females (mean = 0.011 mg/mg of dry weight) to males (mean = 0.010 mg/mg) and considerably diminished in relation to virgin females (mean = 0.005 mg/mg). Significant concentrations of palasonin (21.69 ng/mg among virgins and 17.49 ng/mg in mated females) and palasoninimide (14.62 ng/mg in virgins and 9.17 ng/mg in mated females) were found in H. scabiosae. Palasonin, palasoninimide and cantharidinimide content of eggs were measured as 5.61, 7.69 and 7.80 ng/mg respectively. Surprisingly, males showed no trace of cantharidin-related compounds...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cantharidin; Meloidae; Blister beetle; Hycleus; Chemical defense.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100011

Interaction between infection, nutrition and immunity in tropical medicine
Provedor de dados: 80
Autores: Pereira,P. C. M.
Host nutritional state has an important role in susceptibility to bacteria, parasites, and viral infections. Infection precipitates the appearance of signs and symptoms of nutrition deficiencies in the undernourished; this can aggravate infection evolution. Infection stimulates specific and non-specific host defense mechanisms; these are directly influenced by the nutritional state of micro- and macronutrients. Immune alterations, which are closely related to nutritional status, markedly contribute to a higher susceptibility to infectious agents and can also contribute to worsening nutritional state, forming a vicious cycle.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infection; Nutrition; Immunity; Tropical diseases.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200003

Age-related biological activity of South American rattlesnake (Crotalus durissus terrificus) venom
Provedor de dados: 80
Autores: Furtado,M. F. D.; Santos,M. C.; Kamiguti,A. S.
An in vitro and in vivo comparative study was performed on the effects of Crotalus durissus terrificus venoms from a mother and its 15 newborns. The venoms were tested for protein content, lethality, proteolytic, myotoxic, hemorrhagic, and phospholipase A2 activity. The minimum coagulant dose in plasma and human fibrinogen, protrhombin, and Factor II activations were analyzed. The venoms were also analyzed by polyacrylamide gel electrophoresis (PAGE). This showed that despite similar total protein content, the biological effects of the venoms were different. Venom from young snakes exhibited higher enzymatic and coagulant activities and higher myotoxicity compared to the mother’s. In addition, the PLA2 content paralleled myotoxicity. However, no difference...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Age-related; Biological activities; Rattlesnake venom; Crotalus durissus terrificus.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200005

Massive attack by honeybees in a German shepherd dog: description of a fatal case and review of the literature
Provedor de dados: 80
Autores: Mughal,Mudassar Niaz; Abbas,Ghazanfar; Saqib,Muhammad; Muhammad,Ghulam.
In the present study, a fatal case caused by honeybee (Apis cerana) stings was documented in a female German shepherd dog that was presented at the Veterinary Teaching Hospital, University of Agriculture Faisalabad, Pakistan. Characteristic clinical signs included hematuria, hematemesis, incoordination and convulsions along with evidence of massive honeybee attack supported the diagnosis of envenomation. The dog was treated with dexamethasone and diphenhydramine, but it did not respond to therapy and died. This outcome could be avoided if we had a bee antivenom available for treating envenomated patients.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: German shepherd; Honey bee; Sting.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200510

The inhibitory effect of MK886 on the inflammatory process caused by Paracoccidioidomycosis brasiliensis infection in genetically selected mice
Provedor de dados: 80
Autores: Balderramas,H. A.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100019

Checklist of helminths from lizards and amphisbaenians (Reptilia, Squamata) of South America
Provedor de dados: 80
Autores: Ávila,RW; Silva,RJ.
A comprehensive and up to date summary of the literature on the helminth parasites of lizards and amphisbaenians from South America is herein presented. One-hundred eighteen lizard species from twelve countries were reported in the literature harboring a total of 155 helminth species, being none acanthocephalans, 15 cestodes, 20 trematodes and 111 nematodes. Of these, one record was from Chile and French Guiana, three from Colombia, three from Uruguay, eight from Bolivia, nine from Surinam, 13 from Paraguay, 12 from Venezuela, 27 from Ecuador, 17 from Argentina, 39 from Peru and 103 from Brazil. The present list provides host, geographical distribution (with the respective biome, when possible), site of infection and references from the parasites. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cestoda; Nematoda; Trematoda; Squamata; Neotropical.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400005

Anti-leptospirosis agglutinins in Brazilian capybaras (hydrochoerus hydrochaeris)
Provedor de dados: 80
Autores: Langoni,Helio; Kuribara,Ivone Yumi; Correa,Ana Paula Ferreira Lopes; Ullmann,Leila Sabrina; Sánchez,Gabriela Pacheco; Lucheis,Simone Baldini.
Abstract  Background  The interest in commercial use of wild animals is increasing, especially regarding raising of capybaras. Although this wild species is potentially lucrative for the production of meat, oil and leather, it is suggested as a probable reservoir of leptospires.  Methods  Due to the economic importance of this species and the lack of studies concerning leptospirosis, the presence of anti-leptospirosis agglutinins was assayed in 55 serum samples of capybaras (Hydrochoerus hydrochaeris) from commercial and experimental breeding flocks located in São Paulo state, Paraná state, and Rio Grande do Sul state, Brazil. Samples were obtained through cephalic or femoral venipunction (5 to 10 mL). Microscopic agglutination test was used according to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Capybaras; Hydrochoerus hydrochaeris; Serologic evaluation; Zoonosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100304

The Journal of Venomous Animals and Toxins including Tropical Diseases (JVATiTD) from 1995 to 2007
Provedor de dados: 80
Autores: Barraviera,Benedito.
Tipo: Info:eu-repo/semantics/other
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200001

Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina
Provedor de dados: 80
Autores: Bustillo,S; Lucero,H; Leiva,LC; Acosta,O; Kier Joffé,EB; Gorodner,JO.
Bothrops snake venoms have been proved toxic to a variety of cell types, in both in vivo and in vitro models. Studies on the pharmacological actions of Bothrops venoms from Argentina are relatively scarce and the direct action of the crude venoms has not been assessed using cell culture models. In this work, we investigated the cytotoxicity of crude venoms from B. alternatus and B. diporus in a skeletal muscle (C2C12) cell line, which is commonly used as a model for studying the myotoxic action of snake venom. Both venoms (1.25-50 µg/mL) induced an early and significant decrease in cell viability. The cytotoxic concentration 50 (CC50), determined three hours after exposure, revealed that B. diporus venom was significantly more cytotoxic (CC50: 2 µg/mL)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops alternatus; Bothrops diporus; Cytotoxicity; C2C12; Apoptosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100004

Comparative study of Lacazia loboi inoculation in BALB/c and B10: a mice according to histopathological aspects of produced lesions, number of fungi, and viability index
Provedor de dados: 80
Autores: Madeira,S.
Tipo: Info:eu-repo/semantics/other
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300012

Death of Boa constrictor amarali (serpentes, boidae) after ingestion of a tree porcupine (rodentia)
Provedor de dados: 80
Autores: Cherubini,A. L.; Barrella,T. H.; Silva,R. J. da.
The objective of this paper is to report the death of a Boa constrictor amarali after ingestion of a tree porcupine. The animal was donated to the Center for the Study of Venoms and Venomous Animals (CEVAP/UNESP) - and died in captivity. At necropsy, spine-like structures were observed in the stomach serosa and vicinity, and the stomach mucosa showed an intense reddish area, suggesting inflammation. The analysis of the spine-like structure revealed that they were tree porcupine spines. The feeding habits and inexperience of this Boa constrictor amarali in selecting its prey may have been be responsible for its death.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Serpente; Boidae; Porcupine; Feeding.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100009

Teaching in the XXI century
Provedor de dados: 80
Autores: Biruel,Elisabeth Peres.
Tipo: Info:eu-repo/semantics/other
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300001

Immune response to Mycobacterium bovis-AN5 infection in genetically selected mice (selection IV-A)
Provedor de dados: 80
Autores: Cavaleiro,J. S.; Trindade,B. C.; Balderramas,H. A.; Haanwinckel,M. C.; Pinto,J. G. G.; Oliveira,S. L.; Paes,A. C.
Mice genetically selected for high (H) and low (L) antibody production (Selection IV-A) were used as murine experimental model. The aim of the present work was to evaluate the macrophagic activity and to characterize the immune response in Mycobacterium bovis-AN5 infected mice (3X10(7) bacteria). The response profile previously observed in such strains was not similar to that obtained during M. bovis infection; however, it corroborated works carried out using Selection I, which is very similar to Selection IV-A regarding infection by M. tuberculosis and Bacillus Calmette-Guérin (BCG). Considering bacterial recovery, L IV-A mice showed higher control of the infectious process in the lungs than in the spleen, whereas H IV-A mice presented more resistance in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immune response; Mycobacterium bovis; Biozzi mice.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300006

ALT-C, a disintegrin-like Cys-rich protein from Bothrops alternatus, increases skeletal myoblast viability
Provedor de dados: 80
Autores: Mesquita-Ferrari,RA; Moraes,CK de; Micocci,KC; Selistre-de-Araújo,HS.
ALT-C, an ECD motif (glutamic acid, cysteine, aspartic acid) disintegrin from Bothrops alternatus snake venom, induces α2β1 integrin-mediated signaling and neutrophil chemotaxis. In vitro, in human umbilical vein endothelial cells (HUVEC), ALT-C induces cell proliferation, thus showing an interesting potential for tissue regeneration studies. This work aimed to evaluate the influence of ALT-C in myoblast viability and differentiation. Myoblasts were obtained from hind limb muscles of 3 to 4-day old Wistar rats. The cells were incubated with ALT-C at different concentrations and incubation periods were followed by total RNA isolation. cDNA synthesis and real time polymerase chain reaction (PCR) were performed with primers of myoD as well as of both (slow...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Viability; Myosin heavy chain; Myoblast; Disintegrin; Skeletal muscle; Bothrops alternatus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200013

Schistosomiasis haematobia in Brazilian patients: clinical and renal functional evaluation with 99mTc-DTPA
Provedor de dados: 80
Autores: D’Almeida,J.; Maliska,C.; Pellegrini,P. M.; Penas,M. E.; Collares,R. A.; Cruz,M. G. A.
The present study was carried out at the Army Central Hospital, Rio de Janeiro, Brazil, from September 2000 to December 2001, employing diethylenetriamine penta-acetic acid labeled with technetium-99m (99mTc-DTPA) to evaluate the renal function of nineteen symptomatic patients infected with S. haematobium during a peace mission in Mozambique. Results evidenced that the most frequent clinical manifestations were hematuria (68.4%) and low back pain (68.4%) and 73.7% patients had altered dynamic renal scintigraphy expressed by an increase in the excretory phase independently of the symptoms duration; furthermore, none of them had mechanical obstructive pattern. Schistosoma haematobium glomerulopathy could be considered a pathological finding without...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Schistosomiasis; Schistosoma haematobium; Renal radionuclide imaging; Brazilian patients; 99mTc-DTPA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300010

Screening the toxicity and toxin content of blooms of the cyanobacterium Trichodesmium erythraeum (Ehrenberg) in northeast Brasil
Provedor de dados: 80
Autores: Proença,LAO; Tamanaha,MS; Fonseca,RS.
Blooms of the cyanobacterium Trichodesmium occur in massive colored patches over large areas of tropical and subtropical oceans. Recently, the interest in such events has increased given their role in major nitrogen and carbon dioxide oceanic fluxes. Trichodesmium occurs all along the Brazilian coast and patches frequently migrate towards the coast. In this paper we screen the toxicity and toxin content of Trichodesmium blooms off the coast of Bahia state. Four samples, collected from February to April 2007, were analyzed. Organisms were identified and assessed for toxicity by means of several methods. Analogues of microcystins, cylindrospermopsins and saxitoxins were analyzed using HPLC. Microcystins were also assayed through ELISA. Results showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saxitoxin; Microcystin; Harmful algae; Trichodesmium; Bloom.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200004

A review on the Scorpaena plumieri fish venom and its bioactive compounds
Provedor de dados: 80
Autores: Campos,Fabiana V.; Menezes,Thiago N.; Malacarne,Pedro F.; Costa,Fábio L. S.; Naumann,Gustavo B.; Gomes,Helena L.; Figueiredo,Suely G.
Abstract The most poisonous fish species found along the Brazilian coast is the spotted scorpionfish Scorpaena plumieri. Though hardly ever life-threatening to humans, envenomation by S. plumieri can be quite hazardous, provoking extreme pain and imposing significant socioeconomic costs, as the victims may require days to weeks to recover from their injuries. In this review we will walk the reader through the biological features that distinguish this species as well as the current epidemiological knowledge related to the envenomation and its consequences. But above all, we will discuss the challenges involved in the biochemical characterization of the S. plumieri venom and its compounds, focusing then on the successful isolation and pharmacological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpionfish; Scorpaena plumieri venom; Inflammatory response; Proteolytic activity; Cardiovascular activity; Sp-GP; Plumieribetin; C-type lectins; Sp-CTx.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100205

Modulatory effects of tuberculosis vaccines on experimental autoimmune encephalomyelitis
Provedor de dados: 80
Autores: Zorzella-Pezavento,S. F. G.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100018

Antimicrobial activity of propolis and essential oils and synergism between these natural products
Provedor de dados: 80
Autores: Probst,IS; Sforcin,JM; VLM,Rall; Fernandes,AAH; Fernandes Júnior,A.
In the present study, Apis mellifera propolis and essential oils (EOs) obtained from aromatic plants were evaluated as alternative antimicrobials. We aimed to establish the antimicrobial activity of ethanolic extracts of propolis (EEP) from Apis mellifera and of EOs from Caryophyllus aromaticus, Zingiber officinale, Cinnamomum zeylanicum and Mentha piperita against 32 Staphylococcus aureus and Escherichia coli strains from human clinical specimens. The antimicrobials were diluted in agar and the minimal inhibitory concentrations (MIC) were found whereas MIC90% values were calculated. Time-kill curve assays using mixtures containing one quarter of MIC90% for EEP with all EOs as well cinnamon EO were performed. The cinnamon EO was found to be the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apis mellifera; Aromatic plants; Ethanolic extract of propolis; Staphylococcus aureus; Escherichia coli.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200006

Interleukin 8 (-251 T&amp;gt;A) polymorphism in children and teenagers infected with Helicobacter pylori
Provedor de dados: 80
Autores: Saes,Marina; Labio,Roger Willian de; Rasmussen,Lucas Trevizani; Payão,Spencer Luiz Marques.
Abstract  Background Helicobacter pylori (H. pylori) is a gram-negative bacterium that colonizes the human stomach and causes a variety of gastric diseases. This study evaluated the correlations between the -251 (T&gt;A) (rs4073) polymorphism of interleukin-8 (IL-8), the etiology of gastric disease, and H. pylori infection in pediatric and adolescent patients.  Methods  DNA samples were obtained from 285 gastric biopsies from pediatric patients. H. pylori was detected by PCR, whereas PCR-RFLP was used to characterize the -251 (T&gt;A) polymorphism of IL-8.  Results  The histological analysis revealed the presence of gastritis in 158 patients (55.44%). H. pylori was found in 71 samples (24.9%). The -251 (T&gt;A) polymorphism revealed that 58 (29.47%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori Interleukin 8 Polymorphism -251 (T&amp;gt; A) Gastric disease.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100601

Cytokine production in experimental paracoccidioidomycosis of murine selected lineages for acute inflammatory response (AIR)
Provedor de dados: 80
Autores: Cavalheiro,J. S.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100020

Epidemiological study of scorpion stings in Saudi Arabia between 1993 and 1997
Provedor de dados: 80
Autores: Al-Sadoon,M. K.; Jarrar,B. M.
This investigation evaluated the epidemiological aspects of scorpion stings in different areas of Saudi Arabia. A total of 72,168 cases of scorpion stings recorded in Ministry of Health Medical Centers in 11 selected areas of Saudi Arabia were analyzed based on area, age, sex, time of sting, sting site, treatment outcome, time of year, and scorpion species. Stings occurred throughout the year; the highest frequency was in June (15.08%), the lowest in February (2.52%). Most patients were male (61.8%); the majority of which were more than 15 years old (65.4%). Nocturnal envenomation (47.74%) was more common than diurnal (43.91%); most stings were in exposed limbs (90.95%), mainly in the lower limbs (63%). Most envenomings were mild (74.48%) and all evolved...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion envenomation; Epidemiology; Saudi Arabia.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100003

Leishmania (Viannia) braziliensis is the main species causing cutaneous leishmaniasis in the Federal District of Brazil
Provedor de dados: 80
Autores: Santos,GM; Kückelhaus,SA; Roselino,AM; Chaer,WK; Sampaio,RNR.
The first autochthonous case of American cutaneous leishmaniasis was reported in the Federal District in 1980, and the species involved in this type of leishmaniasis was unknown. This study aimed to identify the species that causes the disease in the Federal District and to investigate its clinical and epidemiological aspects. Between 2000 and 2007, 71 autochthonous cases of leishmaniasis were reported in the Federal District. Leishmania species were identified by means of direct immunofluorescence reactions using monoclonal antibodies and restriction fragment length polymorphism. The species of 40 (56.33%) out of 71 samples were identified. Thirty-six (90%) were identified as Leishmania (Viannia) braziliensis and four (10%) were identified as Leishmania...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: American cutaneous leishmaniasis; Leishmania Viannia braziliensis; Federal District; Autochthonous cases.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300012

Cardiopulmonary complications induced by Iranian Mesobuthus eupeus scorpion venom in anesthetized rabbits
Provedor de dados: 80
Autores: Zayerzadeh,E; Koohi,MK; Zare Mirakabadi,A; Purkabireh,M; Kassaaian,SE; Rabbani,SH; Sotoudeh Anvari,M; Boroumand,MA; Sadeghian,S.
Scorpion envenomation is a life-threatening condition, especially in children and elderly individuals affected by respiratory and cardiovascular diseases. In this study, the toxic effects of median lethal dose (LD50) injections of Mesobuthus eupeus (Me) venom on the heart and lungs of anesthetized rabbits were investigated. Six rabbits were selected and alterations in their electrocardiogram, heart rate, respiration and blood pressure before and after venom injection were recorded. Cardiac troponin T (cTnT), creatinine kinase muscle-brain fraction (CK-MB) and lactate dehydrogenase (LDH) were measured at 0, 1 and 3 hours after envenomation and pathology studies were carried out postmortem. All the animals showed signs and symptoms of envenomation within 40...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesobuthus eupeus; Scorpion experimental envenomation; Histopathological changes; Electrocardiogram changes; Troponin.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100006

Scorpion envenomation symptoms in pregnant women
Provedor de dados: 80
Autores: Ben Nasr,H.; Hammami,T. S.; Sahnoun,Z.; Rebai,T.; Bouaziz,M.; Kassis,M.; Zeghal,K. M.
Scorpion envenomation is common in many countries; however, its effects on pregnancy are still unclear. In the present paper, we described the effects of scorpion envenomation on pregnant patients. A retrospective study was carried out considering the clinical and laboratory exams of patients admitted to the emergency room of Habib Bourguiba Hospital, Sfax, Tunisia, from 1990 to 2004. Variability of these clinical and laboratory profiles according to maternal age, gestational age and number of previous parities was also discussed. Among 167 scorpion-envenomed women, age ranged from 17 to 42 years, 7.18% were pregnant. These presented symptoms similar to those of non-pregnant women envenomed by scorpions. Two pregnant patients developed intense pelvic pain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pregnant women; Scorpion envenomations; Signs and symptoms; Laboratory data.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100007

Scorpions from the primeval subgenus Archaeotityus produce putative homologs of Tityus serrulatus toxins active on voltage-gated sodium channels
Provedor de dados: 80
Autores: Borges,A; Jowers,MJ; Bónoli,S; De Sousa,L.
It has been proposed that the subgenus Archaeotityus comprises the most ancient species group within the medically important scorpion genus Tityus. cDNA encoding sodium-channel active toxins from the type species of this subgenus, Tityus clathratus (central Venezuela), have been isolated and sequenced. Two cDNAs were retrieved that encoded 61 amino acid-long putative neurotoxins named Tcl1 and Tcl2. Sequence identity was highest (87%) when both were compared with β-toxin Ts1 from the Brazilian scorpion Tityus serrulatus and its homologs from T. bahiensis, T. stigmurus, and T. costatus. A Bayesian analysis indicated statistical support for the grouping of T. clathratus Tcl1 and Tcl2 with Brazilian gamma-like β-toxins, reinforcing previous phylogenetic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Archaeotityus; Scorpions; Scorpion toxins; Tityus; Tityus clathratus; Tityus serrulatus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400012

Effects of Mlx-8, a phospholipase A2 from Brazilian coralsnake Micrurus lemniscatus venom, on muscarinic acetylcholine receptors in rat hippocampus
Provedor de dados: 80
Autores: Santos,Roberta Tancredi Francesco dos; Silva,Marcelo Florencio Passos; Porto,Rafael Marques; Lebrun,Ivo; Gonçalves,Luís Roberto de Camargo; Batista,Isabel de Fátima Correia; Sandoval,Maria Regina Lopes; Abdalla,Fernando Maurício Francis.
Abstract  Background:  Here, we described the presence of a neurotoxin with phospholipase A2 activity isolated from Micrurus lemniscatus venom (Mlx-8) with affinity for muscarinic acetylcholine receptors (mAChRs).  Methods:  The purification, molecular mass determination, partial amino acid sequencing, phospholipase A2 activity determination, inhibition of the binding of the selective muscarinic ligand [3H]QNB and inhibition of the total [3H]inositol phosphate accumulation in rat hippocampus of the Mlx-8 were determined.  Results:  Thirty-one fractions were collected from HPLC chromatography, and the Mlx-8 toxin was used in this work. The molecular mass of Mlx-8 is 13.628 Da. Edman degradation yielded the following sequence:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscarinic receptors; Hippocampus; Micrurus lemniscatus; Inositol phosphate; Phopholipase A2.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100301

From the perception of a cluster of cases of children with microcephaly to congenital Zika syndrome in Brazil: the lessons we have learned and the challenges that lie ahead of us
Provedor de dados: 80
Autores: Ribeiro,Erlane Marques; Lopes,Thayse Figueiredo; Kerbage,Sáile Cavalcante; Pessoa,André Luis Santos; Cavalcanti,Luciano Pamplona de Góes.
Tipo: Info:eu-repo/semantics/other
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100801

TNF-α and IL-6 immunohistochemistry in rat renal tissue experimentaly infected with Leptospira interrogans serovar Canicola
Provedor de dados: 80
Autores: Marinho,M; Monteiro,CMR; Peiró,JR; Machado,GF; Oliveira-Júnior,IS.
Leptospirosis is a public health problem worldwide and its etiology remains unclear. Its pathogenesis involves a complex interaction between host and infecting microorganism. The inflammatory reaction that controls the infection process also underscores many pathophysiological events occurring in leptospirosis. We investigated the presence of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in renal tissues by immunohistochemical and histopathological examination in animals experimentally inoculated with Leptospira serovar Canicola. All the tests were carried out 2, 7, 14, 21 or 28 days after inoculation. Although TNF-α and IL-6 had been detected in tissues throughout the observation period, these cytokines appeared more intensely during the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Immunohistochemistry; IL-6; TNF-α; Mice.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300012

Contribution of ultrasonography to the diagnosis of internal bleeding in snakebite envenomation
Provedor de dados: 80
Autores: Tchaou,Blaise Adelin; Tové,Kofi-Mensa Savi de; Tové,Yolande Sissinto-Savi de; Djomga,Aurélien Tchémaha C.; Aguemon,Abdou-Rahman; Massougbodji,Achille; Chippaux,Jean-Philippe.
Abstract  Background  In Africa, snakebite envenomations are frequently complicated by life-threatening hemorrhagic syndromes. The authors of the present study conducted a prospective analysis at the University Hospital of Parakou (north of Benin) for seven months (January 1 to July 31, 2014) to assess the contribution of ultrasonography to the diagnosis of internal bleedings and management of envenomation.  Methods  An ultrasound examination was performed in all patients with clinical envenomation regardless of its severity. The study involved 32 patients admitted to the ICU of the University Hospital of Parakou.  Results  The average age was 27 ± 13.9 years. The main signs of severity were: prolongation of clotting time (88 %), severe anemia (41 %),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Envenomation; Ultrasound; Internal bleeding; Hemoperitoneum; Hematoma.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100306

Bite by moray eel
Provedor de dados: 80
Autores: Barreiros,JP; Haddad Jr,V.
Injuries caused by moray eels are not a common problem, but are distributed throughout the globe, affecting mainly fishermen while manipulating hooked or netted fish. On a lesser scale, scuba divers and snorkelers, practicing or not spear fishing, are occasional victims of bites. With more than 185 species distributed among 15 genera, mostly in tropical to temperate shallow water, moray eels easily come into contact with humans and occasional injuries are not uncommon. The current study reports one case of moray eel bite and discusses the circumstances in which the accident happened, as well as wound evolution and therapy.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dangerous marine fish; Moray eels; Bites in humans.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300013

Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin
Provedor de dados: 80
Autores: Rádis-Baptista,G.; Moreno,F. B. M. B.; Nogueira,L. L.; Martins,A. M. C.; Toyama,D. O.; Toyama,M. H.; Azevedo Jr,W. F.; Cavada,B. S.; Yamane,T.
Snake venom (sv) C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant similarity in their primary structures with C-type lectins of other animals, and also present a conserved carbohydrate recognition domain (CRD). A very well studied sv C-type lectin is the heterodimeric toxin, convulxin (CVX), from the venoms of South American rattlesnakes, Crotalus durissus terrificus and C. d. cascavella. It consists of two subunits, alfa (CVXalpha , 13.9 kDa) and beta (CVXbeta , 12.6 kDa), joined by inter and intra-chain disulfide bounds, and is arranged in a tetrameric alpha4beta4 conformation. Convulxin is able to activate platelet and induce their aggregation by acting via p62/GPVI collagen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durisssus venom; Snake venom C-type lectin; Homology modeling; Platelet aggregation; Antimicrobial activity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400013

Effect of levofloxacin on intestinal microbiota of patients with community-acquired pneumonia
Provedor de dados: 80
Autores: Silva,A. M. P.
Tipo: Info:eu-repo/semantics/article
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300019

Paracoccidioidomycosis: clinical and epidemiological studies of cases observed in the course of 25 years, in Mato Grosso do Sul, Brazil
Provedor de dados: 80
Autores: Paniago,Anamaria Mello Miranda.
Tipo: Info:eu-repo/semantics/article
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300016

Comparison of risk factors among blood donors, volunteers and replacement individuals, infected or not by hepatitis C virus
Provedor de dados: 80
Autores: Felippe,MJDB; Meira,DA.
Hepatitis C is transmitted primarily parenterally by contaminated blood and is often associated with: intravenous drug abuse, invasive procedures, blood transfusions, acupuncture, tattooing, and alcohol and tobacco use. This study aimed to quantify and evaluate the risk factors among blood donors, volunteer blood donors and replacement individuals, infected or not by the C virus. The main transmission routes of C virus were identified in 55 men and 25 women (GI) monitored by the Ambulatory Unit of the Department of Tropical Diseases, Botucatu Medical School, and in 24 men and 26 women (GII), all active blood donors at the Bauru State Hospital Transfusional Agency. Both groups were similar in: tobacco and alcohol consumption, sexual behavior, tattooing and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C; Risk factors; Blood donors; Injectable drugs; Tobacco; Alcohol; Marijuana; Sexual behavior.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100010

Scorpion stings in one province of Morocco: epidemiological, clinical and prognosis aspects
Provedor de dados: 80
Autores: Soulaymani Bencheikh,R.; Idrissi,M.; Tamim,O.; Semlali,I.; Mokhtari,A.; Tayebi,M.; Soulaymani,A.
The present study aimed at verifying the impact of a Moroccan strategy against scorpion stings and, specifically, at identifying the epidemiological features of the patients envenomed or just stung by scorpions. The investigation included 4089 patients from a province of Morocco which were evaluated over three years (2001, 2002 and 2003). Most stings occurred during the hot period and mainly at night (between 6:00 p.m. and 12:00 p.m.). The average incidence was 2.8‰, the average age of the patients was 26.7±18.2 years, and the envenomation rate was 6.7%. Mortality rate was 0.05‰, and average lethality rate was 0.7%. Analysis of variance showed that young age, symptoms at admission, and long time elapsed between sting and admission were correlated with poor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epidemiology; Scorpions; Strategy; Morocco.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200005

Hepatitis C therapy: now more than a coin toss to achieve the cure
Provedor de dados: 80
Autores: An,Barbosa.
Tipo: Info:eu-repo/semantics/other
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100001

Conus vexillum venom induces oxidative stress in Ehrlich's ascites carcinoma cells: an insight into the mechanism of induction
Provedor de dados: 80
Autores: Abdel-Rahman,Mohamed A; Abdel-Nabi,Ismail M; El-Naggar,Mohamed S; Abbas,Osama A; Strong,Peter N.
Background It is estimated that venoms of marine cone snails (genus Conus) contain more than 100,000 different small peptides with a wide range of pharmacological and biological actions. Some of these peptides were developed into potential therapeutic agents and as molecular tools to understand biological functions of nervous and cardiovascular systems. In this study we examined the cytotoxic and anticancer properties of the marine vermivorous cone snail Conus vexillum (collected from Hurgada and Sharm El-Shaikh, Red Sea, Egypt) and suggest the possible mechanisms involved. The in vitro cytotoxic effects of Conus venom were assessed against Ehrlich’s ascites carcinoma (EAC) cells. Results Conus venom treatment resulted in concentration-dependent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conus vexillum venom; Ehrlich’s cells; Oxidative stress; Cancer; Egypt.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100307

Allium species poisoning in dogs and cats
Provedor de dados: 80
Autores: Salgado,BS; Monteiro,LN; Rocha,NS.
Dogs and cats are the animals that owners most frequently seek assistance for potential poisonings, and these species are frequently involved with toxicoses due to ingestion of poisonous food. Feeding human foodstuff to pets may prove itself dangerous for their health, similarly to what is observed in Allium species toxicosis. Allium species toxicosis is reported worldwide in several animal species, and the toxic principles present in them causes the transformation of hemoglobin into methemoglobin, consequently resulting in hemolytic anemia with Heinz body formation. The aim of this review is to analyze the clinicopathologic aspects and therapeutic approach of this serious toxicosis of dogs and cats in order to give knowledge to veterinarians about Allium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium spp.; Poisonous plants; Hemolytic anemia; Heinz bodies.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100002

Isolation and biochemical characterization of bradykinin-potentiating peptides from Bitis gabonica rhinoceros
Provedor de dados: 80
Autores: Fucase,Tamara M.; Sciani,Juliana M.; Cavalcante,Ingrid; Viala,Vincent L.; Chagas,Bruno B.; Pimenta,Daniel C.; Spencer,Patrick J.
Abstract  Background: Venoms represent a still underexplored reservoir of bioactive components that might mitigate or cure diseases in conditions in which conventional therapy is ineffective. The bradykinin-potentiating peptides (BPPs) comprise a class of angiotensin-I converting enzyme (ACE) inhibitors. The BPPs usually consist of oligopeptides with 5 to 13 residues with a high number of proline residues and the tripeptide Ile-Pro-Pro (IPP-tripeptide) in the C-terminus region and have a conserved N-terminal pyroglutamate residue. As a whole, the action of the BPPs on prey and snakebite victims results in the decrease of the blood pressure. The aim of this work was to isolate and characterize novel BPPs from the venom of Bitis gabonica rhinoceros....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peptide; Hypotension; Viperinae.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100322

Antiproliferative activity of marine stingray Dasyatis sephenvenom on human cervical carcinoma cell line
Provedor de dados: 80
Autores: Rajeshkumar,RK; Vennila,R; Karthikeyan,S; Prasad,N Rajendra; Arumugam,M; Velpandian,T; Balasubramaniam,T.
AbstractBackgroundVenoms comprise mixtures of numerous bioactive compounds that have a wide range of pharmacologic actions. Toxins from venomous animals have attracted the attention of researchers because of their affinity for primary sites responsible for lethality and their efficacy at extremely low concentrations. The venoms of marine stingrays have not been extensively studied and limited data is available on them. The present study aims to evaluate the antiproliferative and biochemical properties of the venom obtained from a species of marine stingray (Dasyatis sephen) on human cervical cancer cell line HeLa.MethodsThe antiproliferative effect of D. sephen venom was determined by MTT assay, and the oxidative stress was determined by lipid peroxidation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marine organisms; Membrane potential; Oxidative stress; ROS; Stingray; Venom.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100345

Biological characterization of a myotoxin phosphoplipase A2 homologue purified from the venom of the snake Bothrops moojeni
Provedor de dados: 80
Autores: Queiroz,MR; Mamede,CC; Fonseca,KC; Canabrava,LCMN; França,LV; Silva,MC; Stanziola,L; Beletti,ME; Canabrava,HAN; Oliveira,F.
A myotoxin phospholipase A2 homologue, BmooMtx, was isolated from the venom of Bothrops moojeni by a combination of ion-exchange chromatography on DEAE-Sephacel column and gel filtration on Sephadex G-75. SDS-PAGE showed the enzyme to be a monomer with a molecular weight of 16,500. BmooMtx induced release of creatine kinase and morphological analyses indicated that it provoked an intense myonecrosis, with visible leukocyte infiltrate and damaged muscle cells 24 hours after injection. Anti-BmooMTx antibodies partially neutralized the myotoxic activity of BmooMtx and crude B. moojeni venom, as judged by determination of plasma creatine kinase levels and histological evaluation of skeletal muscle in mice. Anti-BmooMTx antibodies were effective in reducing the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops moojeni; Hyperalgesia; Myotoxin; Edema.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100007

In vitro characterization of jellyfish venom fibrin(ogen)olytic enzymes from Nemopilema nomurai
Provedor de dados: 80
Autores: Bae,Seong Kyeong; Lee,Hyunkyoung; Heo,Yunwi; Pyo,Min Jung; Choudhary,Indu; Han,Chang Hoon; Yoon,Won Duk; Kang,Changkeun; Kim,Euikyung.
Abstract  Background: Because jellyfish are capable of provoking envenomation in humans, they are considered hazardous organisms. Although the effects of their toxins are a matter of concern, information on the venom components, biological activity and pathological mechanisms are still scarce. Therefore, the aim of the present study was to investigate a serine protease component of Nemopilema nomurai jellyfish venom (NnV) and unveil its characteristics.  Methods: To determine the relationship between fibrinolytic activity of NnV and the serine protease, fibrin zymography was performed using metalloprotease and serine protease inhibitors. The biochemical characterization of serine proteases of NnV were determined by the amidolytic assay. Fractions with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nemopilema nomurai; Jellyfish venom; Chymotrypsin; Serine protease; Fibrinolytic activity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100323

Envenomation by the red-tailed coral snake (Micrurus mipartitus) in Colombia
Provedor de dados: 80
Autores: Cañas,Carlos A.; Castro-Herrera,Fernando; Castaño-Valencia,Santiago.
Abstract  Background  Although the red-tailed coral snake (Micrurus mipartitus) is widely distributed in Colombia and its venom is highly neurotoxic and life threatening, envenomation by this species is rare. Therefore, this report may shed some light on the clinical presentation of M. mipartitus bites.  Case presentations  Herein, we describe two cases of patients bitten by red-tailed coral snakes, illustrating the clinical presentation of the victims, the outcomes and treatment provided.  Conclusion  Envenomation caused by M. mipartitus provokes predicable neurotoxicity, and its treatment should be based on respiratory support and use of specific antivenom.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Red-tailed coral snake; Micrurus mipartitus; Snake envenomation; Colombia.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100502

Hump-nosed viper bite: an important but under-recognized cause of systemic envenoming
Provedor de dados: 80
Autores: Shivanthan,Mitrakrishnan Chrishan; Yudhishdran,Jevon; Navinan,Rayno; Rajapakse,Senaka.
Hump-nosed viper bites are common in the Indian subcontinent. In the past, hump-nosed vipers (Hypnalespecies) were considered moderately venomous snakes whose bites result mainly in local envenoming. However, a variety of severe local effects, hemostatic dysfunction, microangiopathic hemolysis, kidney injury and death have been reported following envenoming byHypnalespecies. We systematically reviewed the medical literature on the epidemiology, toxin profile, diagnosis, and clinical, laboratory and postmortem features of hump-nosed viper envenoming, and highlight the need for development of an effective antivenom.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypnale; Hump-nosed viper; Envenoming; Viper; Venom; Antivenom.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200207

Evaluation of surgical scrubbing with 2% chlorhexidine gluconate by the vascular surgery staff
Provedor de dados: 80
Autores: Campos,S. M. C. L.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100020

Interleukin-15 increases Paracoccidioides brasiliensis killing by human neutrophils
Provedor de dados: 80
Autores: Tavian,E. G.
Tipo: Info:eu-repo/semantics/other
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100017

Epidemiological studies in a cutaneous leishmaniasis area in the municipality of Bela Vista, Mato grosso do Sul State, Brazil
Provedor de dados: 80
Autores: Dorval,Maria Elizabeth Moraes Cavalheiros.
Tipo: Info:eu-repo/semantics/article
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100012

Reviewer acknowledgement 2015
Provedor de dados: 80
Autores: Barraviera,Benedito.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100701

Biological properties of medicinal plants: a review of their antimicrobial activity
Provedor de dados: 80
Autores: Silva,NCC; Fernandes Júnior,A.
Plants have been used for thousands of years to flavor and conserve food, to treat health disorders and to prevent diseases including epidemics. The knowledge of their healing properties has been transmitted over the centuries within and among human communities. Active compounds produced during secondary vegetal metabolism are usually responsible for the biological properties of some plant species used throughout the globe for various purposes, including treatment of infectious diseases. Currently, data on the antimicrobial activity of numerous plants, so far considered empirical, have been scientifically confirmed, concomitantly with the increasing number of reports on pathogenic microorganisms resistant to antimicrobials. Products derived from plants may...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants; Phytochemistry; Antimicrobial activity; Plant extracts; Essential oils; Synergism.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300006

Response activity of alveolar macrophages in pulmonary dysfunction caused by Leptospira infection
Provedor de dados: 80
Autores: Marinho,M.; Oliveira-Júnior,I. S.; Perri,S. H.V; Peiró,J. R.; Pavanelli,T. F.; Salomão,R.
Leptopspirosis is a syndrome with different clinical manifestations including the most severe and often fatal forms of pulmonary disease of unknown etiology. Pulmonary injury during the inflammatory process has been associated with the excessive number of alveolar macrophages (AMs) and polymorphonuclear leukocytes stimulated in the lungs and with the production of reactive oxygen and nitrogen intermediates and other inflammatory mediators. The aim of the present work was to evaluate the cellular immune response of AMs or inflammatory cells of hamsters during leptospirosis. The activity of AMs was determined by measuring nitric oxide (NO) and protein production as well as inflammatory cell infiltration in bronchoalveolar lavage (BAL) fluid. Pulmonary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alveolar macrophages; Bronchoalveolar lavage; Gasometry; Pulmonary dysfunction; Hamsters; Leptospira.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100005

Ovine leptospirosis in Brazil
Provedor de dados: 80
Autores: Lucheis,SB; Ferreira Jr.,RS.
Leptospirosis is a zoonosis distributed worldwide, endemic mainly in humid subtropical and tropical countries, with epidemic potential. It affects a range of both wild and domestic animals, including sheep, which transport leptospires in their urine and, therefore, can infect other animals and humans who deal with them. Therefore, leptospirosis is characterized as an occupational zoonosis. In individual herds leptospirosis can cause severe economic loss due to miscarriages and outbreaks of mastitis with a significant reduction of milk production. The disease is caused by Leptospira interrogans, which was reclassified into 13 pathogenic species, and distributed into more than 260 serovars classified into 23 serogroups. The clinical signs of infection may...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sheep; Leptospira; Diagnosis; Animal diseases.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400006

Phyllomedusa bicolor skin secretion and the Kambô ritual
Provedor de dados: 80
Autores: Brave,Paul S den; Bruins,Eugéne; Bronkhorst,Maarten W G A.
The ritual of Kambô or Sapo is a type of voluntary envenomation. During this purification ritual a shaman healer, from various South American countries, deliberately burns the right shoulder with a glowing stick from a fireplace. Excretions of Phyllomedusa bicolor (or Giant Leaf Frog, Kambô or Sapo) are then applied to these fresh wounds. This ritual is used as a means of purification of the body, supposedly brings luck to hunters, increases stamina and enhances physical and sexual strength. All the peripheral and most of the central effects of the secretion can be ascribed to the exceptionally high content of active peptides, easily absorbed through burned skin. This article describes the ritual and the bio-active peptides from the secretion.
Tipo: Info:eu-repo/semantics/other
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200104

Evalution of methods for the detection of MRSA in patients of Botucatu Medical School Hospital, São Paulo, Brazil
Provedor de dados: 80
Autores: Martins,Andre.
Tipo: Info:eu-repo/semantics/article
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300015

Bothrops snake venoms and their isolated toxins, an L-amino acid oxidase and a serine protease, modulate human complement system pathways
Provedor de dados: 80
Autores: Ayres,Lorena Rocha; Récio,Alex dos Reis; Burin,Sandra Mara; Pereira,Juliana Campos; Martins,Andrea Casella; Sampaio,Suely Vilela; Castro,Fabíola Attié de; Pereira-Crott,Luciana Simon.
Background Activation of the complement system plays an important role in the regulation of immune and inflammatory reactions, and contributes to inflammatory responses triggered by envenomation provoked byBothrops snakes. The present study aimed to assess whether Bothrops jararacussuand Bothrops pirajai crude venoms and their isolated toxins, namely serine protease (BjussuSP-I) and L-amino acid oxidase (BpirLAAO-I), modulate human complement system pathways.Methods Lyophilized venom and toxin samples solubilized in phosphate buffered saline were diluted in appropriate buffers to evaluate their hemolytic activity on the alternative and classical pathways of the complement system. Venom- and toxin-treated normal human serum was added to the erythrocyte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararacussu; Bothrops pirajai; Chemotaxis; Complement system; Kinetic microassay; L-amino acid oxidase; Serine protease; Snake venom.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100336

Rotavirus gastroenteritis in a children's hospital specialized in craniofacial malformations
Provedor de dados: 80
Autores: Vieira,NA; Pereira,PCM; Carvalho,LR.
This study aimed to verify the relationship between acute diarrhea provoked by rotavirus and different indicators of craniofacial malformations. In the Hospital for Rehabilitation of Craniofacial Anomalies, 8,724 children with cleft lip and cleft palate were divided into the following groups: acute diarrhea and infection due to rotavirus (C1, n = 62), acute diarrhea (C2, n = 153) and without acute diarrhea (C3, n = 8,509). In C1, 29.03% of the cases consisted of hospital infections associated with the hospitalization period while 38.71% of the patients were aged less than six months. The percentage of children not having breastfed was significantly higher in acute diarrhea groups. Additionally, there was a seasonal prevalence of rotavirus infection between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rotavirus; Gastroenteritis; Diarrhea; Child.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200015

Current challenges for confronting the public health problem of snakebite envenoming in Central America
Provedor de dados: 80
Autores: Gutiérrez,José María.
Snakebite envenoming is a serious public health problem in Central America, where approximately 5,500 cases occur every year. Panama has the highest incidence and El Salvador the lowest. The majority, and most severe, cases are inflicted by the pit viper Bothrops asper (family Viperidae), locally known as ‘terciopelo’, ‘barba amarilla’ or ‘equis’. About 1% of the bites are caused by coral snakes of the genus Micrurus (family Elapidae). Despite significant and successful efforts in Central America regarding snakebite envenomings in the areas of research, antivenom manufacture and quality control, training of health professionals in the diagnosis and clinical management of bites, and prevention of snakebites, much remains to be done in order to further...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Central America; Snakebite; Envenoming; Antivenoms; Public health.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200203

Molecular detection and antimicrobial resistance of Pseudomonas aeruginosa from houseflies (Musca domestica) in Iran
Provedor de dados: 80
Autores: Hemmatinezhad,Behsan; Ommi,Davood; Hafshejani,Taghi Taktaz; Khamesipour,Faham.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial resistance &lt; Italic&gt; Bla&lt; /italic&gt; TEM gene Housefly (Musca domestica) Molecular detection Pseudomonas aeruginosa.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100325

Enzymatic and toxinological activities of Hypnale hypnale (hump-nosed pit viper) venom and its fractionation by ion exchange high performance liquid chromatography
Provedor de dados: 80
Autores: Tan,CH; Sim,SM; Gnanathasan,CA; Fung,SY; Ponnudurai,G; Pailoor,J; Tan,NH.
Hypnale hypnale (hump-nosed pit viper) has been recently identified as one of the medically important venomous snakes in Sri Lanka and on the southwestern coast of India. The characterization of its venom is essential for understanding the pathophysiology of envenomation and for optimizing its management. In the present study, the biological properties of Hypnale hypnale venom and venom fractions obtained using Resource Q ion exchange chromatography were determined. The venom exhibited toxic activities typical of pit viper venom, comparable to that of its sister taxon, the Malayan pit viper (Calloselasma rhodostoma). Particularly noteworthy were its high activities of thrombin-like enzyme, proteases, phospholipase A2, L-amino acid oxidase and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypnale hypnale; Venom; Enzymes; Toxins; Fibrinogen; Nephrotoxicity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400015

Intradermal injection of Bothrops cotiara venom in mice in an experimental wound model
Provedor de dados: 80
Autores: Lopes,JA; Giménez,APL; Zischler,LFCM; Stuelp-Campelo,PM; Moreno,AN; Elifio-Esposito,SL.
Bothropic envenomation induces hemorrhage, coagulant disturbances and necrosis. Regarding therapies against the local damage caused by the venom, there is little information on tissue changes until the complete healing. In the current study, local damage was evaluated by examination of morphological inflammatory alterations, mast cell count, and analysis of collagen deposition. Bleeding was evident four hours after inoculation. After 24 hours, a large area of injury appeared presenting disorganized tissue, significant hemorrhage and acute inflammation. After three days, the damaged area was extensive, with a large amount of inflammatory cells and the presence of scab. In seven days, healing and reepithelization process started. And, 21 days later, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops venom; Wound healing; Inflammation; Local damage.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100009

Application of the HAT-QoL instrument for quality of life analysis with women infected with HIV or AIDS and its correlation with socio-demographic, epidemiological, and clinical variables
Provedor de dados: 80
Autores: Galvão,M. T. G.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100023

Diseases caused by poxvirus - orf and milker's nodules: a review
Provedor de dados: 80
Autores: Barraviera,S.R.C.S.
Sheep and cattle parapoxviruses cause in human beings diseases of very similar aspect, named orf and milker's nodules, respectively. These infections are generically called farmyard pox. In the present article, we show the epidemiological, clinical, and histopathological aspects, as well as the treatment of these two viral diseases that are very similar, being differentiated only by their epidemiological aspects.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orf; Milker's nodules; Poxvirus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200002

Scorpion toxins targeting Kv1.3 channels: insights into immunosuppression
Provedor de dados: 80
Autores: Oliveira,Isadora S; Ferreira,Isabela G; Alexandre-Silva,Gabriel M; Cerni,Felipe A; Cremonez,Caroline M; Arantes,Eliane C; Zottich,Umberto; Pucca,Manuela B.
Abstract Scorpion venoms are natural sources of molecules that have, in addition to their toxic function, potential therapeutic applications. In this source the neurotoxins can be found especially those that act on potassium channels. Potassium channels are responsible for maintaining the membrane potential in the excitable cells, especially the voltage-dependent potassium channels (Kv), including Kv1.3 channels. These channels (Kv1.3) are expressed by various types of tissues and cells, being part of several physiological processes. However, the major studies of Kv1.3 are performed on T cells due its importance on autoimmune diseases. Scorpion toxins capable of acting on potassium channels (KTx), mainly on Kv1.3 channels, have gained a prominent role for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Voltage-gated potassium channels; Kv1.3; Scorpion toxins; KTx; Immunosuppression.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100202

Honey bee attractants and pollination in sweet orange, Citrus sinensis (L.) Osbeck, var. Pera-Rio
Provedor de dados: 80
Autores: Malerbo-Souza,D. T.; Nogueira-Couto,R. H.; Couto,L. A.
This experiment studied the frequency and behavior of insects on sweet orange flowers, Citrus sinensis (L.) Osbeck, their effect on fruit production (quantity and quality), nectar and pollen collection, and the effectiveness of different attractants. Over three consecutive years, the most frequent visitor to the flowers was Apis mellifera (Africanized). Flowers visited less than ten times showed low fructification. Fruit production was 35.30% greater in uncovered flowers. Fruit mean weight was much greater in uncovered (180.2g) than in covered flowers (168.5g). Fruits from the covered were more acid (1.411g of citric acid/100ml of juice) than the uncovered flowers (1.164g of citric acid/100ml of juice). The number of seeds per bud was higher in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Attractants; Citrus sinensis var. Pera-Rio; Pollination; Apis mellifera.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200004

Cardiorespiratory alterations in rodents experimentally envenomed with Hadruroides lunatus scorpion venom
Provedor de dados: 80
Autores: Costal-Oliveira,Fernanda; Guerra-Duarte,Clara; Oliveira,Maira Souza; Castro,Karen Larissa Pereira de; Lopes-de-Sousa,Leticia; Lara,Aline; Gomes,Enéas Ricardo de Morais; Bonilla,Cesar; Guatimosim,Sílvia; Melo,Marília Martins; Chávez-Olórtegui,Carlos.
Abstract  Background Hadruroides lunatus is the most abundant scorpion species in the Peruvian central coast, where most of the accidents involving humans are registered. In spite of its prevalence, there are only very few studies on H. lunatus envenomation. The aim of the present study was to analyze the cardiorespiratory alterations caused by H. lunatus envenomation in rodents.  Methods  Wistar rats injected with H. lunatus scorpion venom were submitted to electrocardiography. After euthanasia, rat lungs were collected and histopathologically analyzed. Mouse cardiomyocytes were used to perform immunofluorescence and calcium transient assays. Data were analyzed by ANOVA or Student’s t-test. The significance level was set at p&lt; 0.05.  Results  It was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hadruroides lunatus venom; Cardiorespiratory alterations; Electrocardiography; Immunofluorescence; Calcium transient.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100301

Snakebites in Turkey: epidemiological and clinical aspects between the years 1995 and 2004
Provedor de dados: 80
Autores: Cesaretli,Y; Ozkan,O.
The venom of poisonous snakes comprises a complex mixture of several proteins with other less significant constituents, resulting in principles capable of changing viable tissues. The hemotoxic factor is the main responsible for necrosis and tissue sloughing. Envenomations are common in rural areas of Turkey caused by snake species that present hepatotoxic venom, which causes local swelling, ecchymosis and alterations in blood profile. The epidemiological and clinical findings of snake envenomations in Turkey were evaluated based on data recorded by the National Poison Information Center (NPIC) between 1995 and 2004, in a total of 550 snakebite cases. The month of peak incidence was June (24.3%) while most incidents occurred in Marmara, Central Anatolia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snakebite; Envenomation; Clinical features; Epidemiology; Turkey.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400007

Who communicates with whom?
Provedor de dados: 80
Autores: Santos,LD.
Tipo: Info:eu-repo/semantics/other
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200001

Seroprevalence of flaviviruses antibodies in water buffaloes (Bubalus bubalis)in Brazilian Amazon
Provedor de dados: 80
Autores: Casseb,Alexandre R; Cruz,Andrea V; Jesus,Iroleide S; Chiang,Jannifer O; Martins,Lívia C; Silva,Sandro P; Henriques,Daniele F; Casseb,Livia MN; Vasconcelos,Pedro Fernando C.
BackgroundThe state of Pará encompasses 26% of Brazilian Amazon where an enormous diversity of arboviruses has been found. This study aimed to assess the prevalence and distribution of hemagglutination-inhibition antibodies against antigens of six Flavivirus(yellow fever virus, Ilheus virus, Saint Louis encephalitis virus, Cacipacore virus, Bussuquara virus and Rocio virus) in water buffaloes in Pará state, Brazil. The prevalence of antibodies in these farm animals is important to determine the circulating arboviruses.FindingsAll investigated arboviruses were detected in the species studied and our results indicate that water buffaloes are susceptible to Flavivirusinfection. Furthermore, there is solid evidence of active circulation of these viruses in the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Flavivirus; Water buffaloes; Hemagglutination inhibition test.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200600

Snakebites notified to the poison control center of Morocco between 2009 and 2013
Provedor de dados: 80
Autores: Chafiq,Fouad; Hattimy,Faiçal El; Rhalem,Naima; Chippaux,Jean-Philippe; Soulaymani,Abdelmajid; Mokhtari,Abdelrhani; Soulaymani-Bencheikh,Rachida.
Abstract  Background  Snakebites cause considerable death and injury throughout the globe, particularly in tropical regions, and pose an important yet neglected threat to public health. In 2008, the Centre Anti Poison et de Parmacovigilance du Maroc (CAPM) started to set up a specific strategy for the control of snakebites that was formalized in 2012. The aim of the present study is to describe and update the epidemiological characteristics of snakebites notified to CAPM between 2009 and 2013.  Methods  This retrospective five-year study included all cases of snakebites notified to CAPM by mail or phone.  Results  During the study period, 873 snakebite cases were reported to CAPM, an average incidence of 2.65 cases per 100,000 inhabitants with 218 cases...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snakebite; Envenomation; Viper; Morocco; Epidemiology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100308

Erratum to: Pediatric suppurative parotitis caused by Burkholderia pseudomallei
Provedor de dados: 80
Tipo: Info:eu-repo/semantics/other
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100901

Presynaptic neuromuscular action of a methanolic extract from the venom of Rhinella schneideri toad
Provedor de dados: 80
Autores: Rostelato-Ferreira,Sandro; Belo,Cháriston A Dal; Leite,Gildo B; Hyslop,Stephen; Rodrigues-Simioni,Léa.
BackgroundRhinella schneideri, previously known as Bufo paracnemis, is a common toad in many regions of Brazil. Its venom exerts important cardiovascular effects on humans and other animals. Although this toad venom has been the subject of intense investigations, little is known about its neuromuscular activity.MethodsThe neurotoxicity of a methanolic extract of R. schneideri venom was tested on mouse phrenic nerve-diaphragm (PND) preparations mounted for conventional twitch tension recording – in response to indirect stimulation – and for electrophysiological measurements.ResultsVenom extract (50 μg/mL) increased the muscle twitch tension in PND preparations but did not significantly alter the resting membrane potential values. Electrophysiological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurotransmitter release; Ouabain; Presynaptic; Rhinella schneideri; Toad venom.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200332

Economic evaluation of snake antivenom production in the public system
Provedor de dados: 80
Autores: Morais,V.; Massaldi,H.
Snake antivenom, an expensive animal product, is presently the only effective treatment for the consequences of snakebite. In Latin America, antivenoms are mainly produced by public institutions with frequent shortages of the necessary supply. Here, we present an economical analysis of the factors affecting production cost, assuming a basic processing batch of 100 L hyperimmune plasma. Three annual production volumes were considered for two typical production technologies. The components of cost were classified as fixed, variable and semi-variable. We found that in all stages of production, fixed cost represents the major contribution to total cost, and is given essentially by manpower cost, particularly for low production volumes. Our estimation shows...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antivenom; Economical cost; Immunoglobulins; Equine plasma; Serum production.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300012

Cleaning behaviors in four scorpion species
Provedor de dados: 80
Autores: Jiao,GB; Zhu,MS.
Scorpions rely predominantly on mechanosensory and chemosensory organs to guide their orientation behaviors. Once sensory organs are affected by the presence of dirt such as clay or prey bodily fluid, scorpions may display a cleaning behavior to reduce or eliminate its influence on their sensory capabilities. In the laboratory, cleaning behaviors of two buthid species, Mesobuthus eupeus (Koch, 1839) and Mesobuthus caucasicus (Nordmann, 1840), and one euscorpiid species, Scorpiops luridus Zhu Lourenço &amp;amp; Qi, 2005 from China, were observed before and after feeding. Moreover, two distinct cleaning behaviors in Scorpiops luridus and three in Heterometrus petersii (Thorell, 1876) (Scorpionidae) were noted for several times during daily activities. Based...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion; Cleaning behavior; Cleaning means; Prey capture; Daily activity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200017

A case of stings in humans caused bySclerodermus sp. in Italy
Provedor de dados: 80
Autores: Papini,Roberto Amerigo.
In the last years, stings of Sclerodermus species in humans have been sporadically reported in Italy. In order to draw attention to these bethylid wasps of medical importance, we report the case of documentedSclerodermus sp. stings on the dorsum, abdomen, arms, and thighs of a 40-year-old man and his wife. The sting sites developed raised red itchy rash. The source of environmental contamination was identified in a worm-eaten sofa purchased from a used furniture dealer and placed in the living room about a month and half earlier. The lesions on the man and his wife rapidly healed within 3 to 4 days once they left the house and treatment for the lesions was instituted. Physicians, dermatologists, medical and public health entomologists, as well as specific...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Sclerodermus; Bethylid wasps; Stings; Italy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200503

Is the population of Crotalus durissus (Serpentes, Viperidae) expanding in Brazil?
Provedor de dados: 80
Autores: Duarte,Marcelo Ribeiro; Menezes,Frederico Alcântara.
Crotalus durissus are found from Mexico to northern Argentina in a highly disjunct distribution. According to some studies, this species is prone to occupy areas disturbed by human activities and floods comprise a plausible method of dispersal as inferred for some North American rattlesnakes. Based on the literature, it seems plausible that Crotalus durissus expanded their natural distribution in Brazil due to floods, but only in a few municipalities in Rio de Janeiro State. Data entries of Butantan Institute, in São Paulo, Brazil, from 1998 to 2012 show a declining tendency of snakes brought by donors. In addition, research shows no evidence of Crotalus durissus being an expanding species in the Brazilian territory.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rattlesnake; Habitat degradation; Species conservation; Animal distribution.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100103

What do we know about some of the most conspicuous scorpion species of the genus Tityus? A historical approach
Provedor de dados: 80
Autores: Lourenço,Wilson R.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion &lt; Italic&gt; Tityus&lt; /italic&gt; Noxious species Historical aspects.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100204

Individual variation in the protein profile of the venom of Mesobuthus gibbosus (Brullé, 1832, Scorpiones: Buthidae) from Turkey
Provedor de dados: 80
Autores: Ozkan,O; Ciftci,G.
Mesobuthus gibbosus (Brullé, 1832) scorpions were collected from the Mugla province in the Aegean region of Turkey and housed in individual boxes. After extraction, the venom composition was analyzed using gel electrophoresis (SDS-PAGE). Interestingly, all scorpion venom samples contained only one protein band (~68 kDa) in common. Two protein bands (30 and 98 kDa) were common in six venom samples and were absent in the other venoms. Furthermore, two different protein bands (28 and 45 kDa) were detected in seven venom samples. This study proposes possible variations in the composition of individual scorpion venom samples collected from the same geographic region, based on the electrophoretic profile. Additional studies will be necessary in order to assess...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Scorpion; Mesobuthus gibbosus; Venom; Protein; Turkey.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300017

"Syndromic surveillance" and the reemergence of yellow fever in São Paulo State, Brazil, 2009
Provedor de dados: 80
Autores: Fortaleza,CMCB; Rocha,R; Aragão,VDN; Almeida,RAMB.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200002

Divergences between the Brazilian national information systems for recording deaths from venomous animals
Provedor de dados: 80
Autores: Bochner,Rosany; Souza,Claudio Mauricio Vieira de.
Abstract  Background: This paper aims to highlight and analyze discrepancies in reporting of deaths due to venomous animals in Brazil, from 2001 to 2015, between two national information systems: The Notifiable Diseases Information System (Sistema de Informação de Agravos de Notificação - SINAN) and the Mortality Information System (Sistema de Informações sobre Mortalidade - SIM).  Methods: Descriptive and comparative study of the SINAN and SIM information systems, was conducted via the following steps: collecting the death notices from SINAN and SIM; constructing tables and comparative graphics; and, only in scorpion sting fatalities, analyzing the distribution of deaths by age group as described in the specialized literature.  Results: While SINAN...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Information system; Death; SINAN; SIM; Snake; Spider; Scorpion; Bee.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100313

The impact-factor neurosis, competitiveness necessity and inattention can cause irreparable damage…
Provedor de dados: 80
Autores: Barraviera,Benedito.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300001

Natural infection of phlebotomines (Diptera: Psychodidae) by Leishmania (Leishmania) amazonensis in an area of ecotourism in Central-Western Brazil
Provedor de dados: 80
Autores: Brilhante,Andreia Fernandes; Nunes,Vânia Lúcia Brandão; Kohatsu,Kleber Augusto; Galati,Eunice Aparecida Bianchi; Rocca,Maria Elizabeth Ghizzi; Ishikawa,Edna Aoba Yassui.
AbstractBackground Bonito municipality, known as an area of ecoturism, in Mato Grosso do Sul state, Brazil, is also a focus of visceral and cutaneous leishmaniases, with cases registered in both human and canine populations. This study sought to investigate natural infection by flagellate forms of Leishmania in phlebotomines of the urban area of Bonito.Findings Sand flies were collected fortnightly from October 2005 to July 2006 with modified automatic light traps installed in peridomiciles and animal shelters in the center and on the outskirts of the city. The females were dissected and their guts observed under an optical microscope. A total of 1977 specimens were captured, Lutzomyia longipalpis (88.4 %) and Bichromomyia flaviscutelata (3.0 %) being the...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Bichromomyia flaviscutellata; Lutzomyia longipalpis; Natural infection; Leishmania (Leishmania) amazonensis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100502

Renal- and calcium-dependent vascular effects of Polybia paulista wasp venom
Provedor de dados: 80
Autores: Vinhote,JFC; Torres,AFC; Dantas,RT; Praciano,TP; Menezes,RRPPB; Sousa,DF; Brito,TS; Lima,FJB; Toyama,MH; Magalhães,PJ; Monteiro,HSA; Martins-Nunes,AMC.
In the present study, the effects of Polybia paulista venom (PPV) on renal and vascular tissues were investigated. Isolated kidneys perfused with PPV (1 and 3 μg/mL) had increased perfusion pressure, renal vascular resistance, urinary flow, and glomerular filtration rate; and reduced sodium tubular transport. Histological evaluation demonstrated deposits of proteins in Bowman's space and tubular lumen, and focal areas of necrosis. The venom promoted a cytotoxic effect on Madin-Darby canine kidney (MDCK) cells. A significant increase in lactic dehydrogenase levels was observed in response to venom exposure. In isolated mesenteric vascular beds, pressure and vascular resistance augmented in a dose-dependent manner. PPV increased the contractility of aortic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polybia paulista; Venom; Kidney; Aorta; MDCK.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200011

Identification of two novel cytolysins from the hydrozoan Olindias sambaquiensis (Cnidaria)
Provedor de dados: 80
Autores: Haddad Junior,Vidal; Zara,Fernando; Marangoni,Sergio; Toyama,Daniela de Oliveira; Souza,Alex Jardelino Felizardo de; Oliveira,Simone Cristina Buzzo de; Toyama,Marcos Hikari.
Background: Although the hydrozoan Olindias sambaquiensis is the most common jellyfish associated with human envenomation in southeastern and southern Brazil, information about the composition of its venom is rare. Thus, the present study aimed to analyze pharmacological aspects ofO. sambaquiensis venom as well as clinical manifestations observed in affected patients. Crude protein extracts were prepared from the tentacles of animals; peptides and proteins were sequenced and submitted to circular dichroism spectroscopy. Creatine kinase, cytotoxicity and hemolytic activity were evaluated by specific methods.Results: We identified two novel cytolysins denominated oshem 1 and oshem 2 from the tentacles of this jellyfish. The cytolysins presented the amino...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Olindias sambaquiensis; Hemolytic; Myonecrosis; Cytotoxicity; Cytolysin; Cnidaria venom.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200316

Clostridium perfringens isolate typing by multiplex PCR
Provedor de dados: 80
Autores: Ahsani,MR; Mohammadabadi,MR; Shamsaddini,MB.
Clostridium perfringens is an important pathogen that provokes numerous different diseases. This bacterium is classified into five different types, each of which capable of causing a different disease. There are various methods for the bacterial identification, many are labor-intensive, time-consuming, expensive and also present low sensitivity and specificity. The aim of this research was to identify the different types of C. perfringens using PCR molecular method. In this study, 130 sheep-dung samples were randomly collected from areas around the city of Kerman, southeastern Iran. After processing and culturing of samples, the produced colonies were morphologically studied, gram stain test was also carried out and the genera of these bacteria were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Multiplex PCR; Biochemical tests; Genetic typing.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400006

Occurrence of pneumocystis pneumonia in hiv-infected patients and the interference of the highly active antiretroviral therapy
Provedor de dados: 80
Autores: Barbosa,A. N.; Souza,L. R.
From the beginning of the AIDS epidemic, pneumocystis pneumonia (PCP) has been distinguished as one of the most frequent opportunistic diseases with high morbid-mortality. As from 1996, the advent of the highly active antiretroviral therapy (HAART) has changed the characteristics of such epidemic by reducing its related diseases and, as a result, AIDS-related mortality. With the purpose to estimate PCP occurrence and HAART interference, 376 HIV-infected or AIDS patients were studied from January 1992 to December 2002. Among them, 58 (15.5%) PCP cases were found. There was a higher occurrence of PCP in the group of patients in which HAART was not used, with 40 (69.0%) of the episodes. As regards the studied period, a tendency to a linear reduction in annual...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AIDS; Pneumocystis pneumonia; Antiretroviral treatment; T CD4+ lymphocytes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100012

Snake venom L-amino acid oxidases: an overview on their antitumor effects
Provedor de dados: 80
Autores: Costa,Tássia R; Burin,Sandra M; Menaldo,Danilo L; Castro,Fabíola A de; Sampaio,Suely V.
The L-amino acid oxidases (LAAOs) constitute a major component of snake venoms and have been widely studied due to their widespread presence and various effects, such as apoptosis induction, cytotoxicity, induction and/or inhibition of platelet aggregation, hemorrhage, hemolysis, edema, as well as antimicrobial, antiparasitic and anti-HIV activities. The isolated and characterized snake venom LAAOs have become important research targets due to their potential biotechnological applications in pursuit for new drugs of interest in the scientific and medical fields. The current study discusses the antitumor effects of snake venom LAAOs described in the literature to date, highlighting the mechanisms of apoptosis induction proposed for this class of proteins.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venoms; L-amino acid oxidases; Antitumor effects; Apoptosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200206

The red seaweed Plocamium brasiliense shows anti-snake venom toxic effects
Provedor de dados: 80
Autores: Silva,Geisiane Alves da; Domingos,Thaisa Francielle Souza; Fonseca,Rainiomar Raimundo; Sanchez,Eladio Flores; Teixeira,Valéria Laneuville; Fuly,André Lopes.
Background Snakebite is considered a neglected tropical disease by the World Health Organization. In Brazil, about 70% of the envenomation cases are caused by Bothrops snakes. Its venom may provoke hemorrhage, pain, necrosis, hemolysis, renal or cardiac failure and even death in victims. Since commercial antivenom does not efficiently neutralize the local toxic effects of venoms, natural products have been tested in order to provide alternative or complementary treatment to serum therapy. Therefore, the present study aimed to evaluate the ability of the seaweed Plocamium brasiliense and its active derivatives to neutralize hemorrhagic, edematogenic, hemolytic, coagulant and proteolytic activities of B. jararaca venom. Methods Specimens of P. brasiliense...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararaca; Snake venom; Plocamium brasiliense; Seaweed; Antivenom; Bioprospecting.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100312

Leptospiral antibodies in wild boars (Sus scrofa) bred in Brazil
Provedor de dados: 80
Autores: Fornazari,F; Camossi,LG; Silva,RC; Guazzelli,A; Ribeiro,MG; Chiacchio,SB; Langoni,H.
In the recent years, the wild boar (Sus scrofa) trade has increased in Brazil. The present study aimed to detect Leptospira spp. infection in 308 blood samples from wild boars bred in São Paulo state, Brazil. The microscopic agglutination test (MAT) was performed using 29 serovars. Sixty three (63; 20.45%) animals tested positive against the serovars Hardjo (29/63; 46.0%), Copenhageni (11/63; 17.4%), Pomona (8/63; 12.7%), Pyrogenes (4/63; 6.3%), Wolfii (4/63; 6.3%), Autumnalis (3/63; 4.7%), Icterohaemorraghiae (2/63; 3.1%), and Hardjo-miniswajezak (1/63; 15.8%). These results indicate that captive wild boars were infected with Leptospira spp. and may represent a source of infection to humans and other animals.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wild boar; Sus scrofa; Leptospira spp.; MAT; Zoonosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100012

Ethnobotanic study of Randia aculeata (Rubiaceae) in Jamapa, Veracruz, Mexico, and its anti-snake venom effects on mouse tissue
Provedor de dados: 80
Autores: Gallardo-Casas,CA; Guevara-Balcázar,G; Morales-Ramos,E; Tadeo-Jiménez,Y; Gutiérrez-Flores,O; Jiménez-Sánchez,N; Valadez-Omaña,MT; Valenzuela-Vargas,MT; Castillo-Hernández,MC.
In Mexico, medicinal plants are widely used. The use of Randia aculeata by healers against snakebites has never been scientifically tested in relation to possible effects on blood parameters and muscle tissue damage. Interviews were carried out in Jamapa, Veracuz, Mexico, with local residents to collect information about the traditional use of Randia aculeata. In this locality, seven pieces of fruit from the plant are mixed in a liter of alcohol, and then administered orally against snakebites. By using histological techniques and a murine model, we explored its cytoprotective properties against the effects of Crotalus simus and Bothrops asper venoms. Possible protections provided by the plant against tissue damage to skeletal and cardiac muscles and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rubiaceae; Antivenoms; Bothrops; Crotalus; Cytoprotection.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300006

Surgery should not be used as first-line treatment
Provedor de dados: 80
Autores: Chippaux,J-P.
Tipo: Info:eu-repo/semantics/article
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100002

Notes on gestation periods and litter size in the arenicolous buthid scorpion Leiurus quinquestriatus (Ehrenberg, 1828) (Scorpiones: Buthidae)
Provedor de dados: 80
Autores: Ross,LK.
The following notes on gestation periods and litter size in seven specimens of Leiurus quinquestriatus are presented as observational data. Specimens of Leiurus quinquestriatus from southern Egypt and southern Israel were mated in the laboratory during 2007; afterwards, gestation periods and litter sizes of all females were recorded. Previous studies on this species reported that the gestation period ranged from 150 to 155 days and that litter size was between 12 and 99 offspring. In the present study, gestation periods in specimens from both geographic regions varied from 155 to 227 days and litter sizes were between 35 and 87 offspring. The current contribution expands on previously published data on gestation periods and supports previously reported...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpions; Buthidae; Leiurus quinquestriatus; Gestation period; Litter size.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200015

Selection of environmental temperature by the yellow scorpion Tityus serrulatus Lutz &amp;amp; Mello, 1922 (Scorpiones, Buthidae)
Provedor de dados: 80
Autores: Hoshino,K.; Moura,A. T. V.; De Paula,H. M. G.
The preferred temperature of the yellow scorpion Tityus serrulatus was investigated since its dispersion is a matter of concern. Adult T. serrulatus, weighing 1.24 + 0.20 g (mean + sd) and with a standard length of 59.3 + 2.5 mm, were used. A metallic corridor (120 cm long, 5 cm large and 10 cm high) with thermal gradient ranging from 0°C to 40°C was used. Tityus serrulatus chose and stayed in temperatures ranging from 14°C to 38°C when safe conditions were offered (dark and thigmotactic stimuli). The number of animals that remained in the 11°C-20°C, 21°C-30°C, and 31°C-40°C temperature zones were 8, 8, and 9, respectively. The chi-square test (degree of freedom = 2) showed that differences were not significant (p&gt;0.05). Some animals moved to lower...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tityus serrulatus; Scorpion; Temperature; Thermal gradient; Zoogeography.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100005

Cytoskeleton, endoplasmic reticulum and nucleus alterations in CHO-K1 cell line after Crotalus durissus terrificus (South American rattlesnake) venom treatment
Provedor de dados: 80
Autores: Tamieti,B. P.; Damatta,R. A.; Cogo,J. C.; Da Silva,N. S.; Mittmann,J.; Pacheco-Soares,C.
Snake venoms are toxic to a variety of cell types. However, the intracellular damages and the cell death fate induced by venom are unclear. In the present work, the action of the South American rattlesnake Crotalus durissus terrificus venom on CHO-K1 cell line was analyzed. The cells CHO-K1 were incubated with C. d. terrificus venom (10, 50 and 100g/ml) for 1 and 24 hours, and structural alterations of actin filaments, endoplasmic reticulum and nucleus were assessed using specific fluorescent probes and agarose gel electrophoresis for DNA fragmentation. Significant structural changes were observed in all analyzed structures. DNA fragmentation was detected suggesting that, at the concentrations used, the venom induced apoptosis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell culture; Actin filaments; Nucleus; Endoplasmic reticulum; Crotalus; Rattlesnake venom; Apoptosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100004

Hand injuries in a human caused by a South American porcupine (ouriço-cacheiro)
Provedor de dados: 80
Autores: Haddad Jr,V; Vieira,RB; Cortes,CR.
Human injuries caused by South American porcupines (in Portuguese, ouriço-cacheiro) are rare. This study reports severe hand injuries provoked by the body spines of the animal in a human and discusses the circumstances involved in the accident, with emphasis on environmental factors.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: South American porcupine; Hand injuries; Body spines; Brazil.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200018

Acutolysin C, a weak hemorrhagic toxin from the venom of Agkistrodon acutus with leucoagglutination activity
Provedor de dados: 80
Autores: Wei,CB; Chen,J; Li,JH.
The properties and agglutination activity of acutolysin C, a hemorrhagic metalloproteinase obtained from Agkistrodon acutus venom, were studied herein. Acutolysin C is a basic glycoprotein consisting of a single polypeptide chain with a molecular weight of 23.1 kDa and pI 8.7, containing one Zn2+ and one Ca²+ per molecule. It possesses caseinolytic, weak lethal (LD50 = 7.6 mg/kg) and weak hemorrhagic (MHD = 12.0 μg) activities, but does not present fibrinolytic, fibrinogenolytic, arginine esterase and phospholipase A2 actions. In addition, it revealed agglutination activity on some animal lymphocytes, including five species of mammals, six of birds, three of reptiles and one of amphibians, but had no effect on lymphocytes from two species of reptiles, one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agkistrodon acutus; Acutolysin C; Hemorrhagin; Leucoagglutination activity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100005

Anti-coagulase-negative Staphylococcus activity of ethanolic extracts of propolis from two Brazilian regions and synergism with antimicrobial drugs by the E-test method
Provedor de dados: 80
Autores: Mantovani,R. P.; Rall,V. L. M.; Batalha,J. E. N.; Fernandes,A. A. H.; Fernandes Junior,A.
Propolis is a natural resinous substance collected by bees from vegetal sources and its therapeutic properties have been investigated. In this work, we evaluated the inhibitory activity of ethanolic extracts of propolis (EEP) from the Southeast and South of Brazil on coagulase-negative Staphylococcus (CNS) growth as well as the EEP in vitro synergism with antimicrobial drugs by using the diffusion method (E-test). The EEP chemical characteristics (dry weight, pH, flavonoid and phenolic compounds) were determined. Seven drugs were tested, and synergism was observed between three drugs and Southeast EEP, six drugs and South EEP, and one drug and ethanol control. Ethanolic extracts of propolis from the South of Brazil presented the greatest flavonoid content...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian propolis; Coagulase-negative Staphylococcus; E-test; Antimicrobial drugs; Synergism.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200012

Antagonistic properties of some microorganisms isolated from Brazilian tropical savannah plants against Staphylococcus coagulase-positive strain
Provedor de dados: 80
Autores: Ratti,R. P.; Serrano,N. F. G.; Hokka,C. O.; Sousa,C. P.
Endophytic microorganisms are relatively unstudied as potential sources of novel natural products for medical and commercial exploitation. The aim of this work was to investigate some Brazilian tropical savannah trees Cassia leptophylla and Prunus spp. in order to isolate the endophytic microorganisms associated with these plants. The samples were disinfected to eliminate the epiphytic population. Colonies were diluted and displayed as drops in media and growing colonies were inactivated. Staphylococcus coagulase-positive strain was used as indicator microorganism and subjected to the antibioses test. Data showed that the microorganisms isolated from Cassia leptophylla had no inhibition against Staphylococcus. On the other hand, microorganisms isolated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophytic microorganisms; Plants; Antibacterial activity; Bioactive compounds.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200007

Samsum ant venom modulates the immune response and redox status at the acute toxic dose in vivo
Provedor de dados: 80
Autores: Ebaid,Hossam; Abdel-Salam,Bahaa; Alhazza,Ibrahim; Al-Tamimi,Jameel; Hassan,Iftekhar; Rady,Ahmed; Mashaly,Ashraf; Mahmoud,Ahmed; Sammour,Reda.
Abstract  Background:  Ant venoms express surface molecules that participate in antigen presentation involving pro- and anti-inflammatory cytokines. This work aims to investigate the expression of MHC-II, CD80 and CD86 on the polymorphonuclear cells (PMNs) in rats injected with samsum ant venom (SAV).  Methods:  Rats were divided into three groups - control, SAV-treated (intraperitoneal route, 600 μg/kg), and SAV-treated (subcutaneous route, 600 μg/kg). After five doses, animals were euthanized and samples collected for analysis.  Results:  The subcutaneous SAV-trated rats presented decreased levels of glutathione with increased cholesterol and triglyceride levels. Intraperitoneal SAV-treated animals displayed significantly reduced concentrations of both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Samsum ant venom; Polymorphonuclear cells (PMNs); Costimulatory molecules (CD80 and CD86); Major histocompatibility complex (MHC); MHC-II; Interferon gamma (INF-γ); Interleukin-17 (IL-17).
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100208

Posterior fossa infarct following Viper bite: a paradox
Provedor de dados: 80
Autores: Deepu,D; Hrishikesh,S; Suma,MT; Zoya,V.
Cerebral infarction after a viper bite is relatively uncommon. A combination of factors has been implicated in the pathophysiology of infarct following snakebite. In this case report, the clinical outcome after a posterior circulation infarct and various possibilities that could lead to such a catastrophic event are discussed. The present study stresses the need to keep hydration, blood pressure and central venous pressure optimal in all snakebite patients. Cerebral infarction should be considered a differential diagnosis, in any patient with neurological deterioration following snakebite. Prognosis of such patients with posterior circulation stroke remains poor and decompressive craniectomy has not been found to be helpful.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snakebite; Cerebral infarction; Russell's viper; Posterior cranial fossa.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300017

Standardization of anti-lethal toxin potency test of antivenoms prepared from two different Agkistrodon halys venoms
Provedor de dados: 80
Autores: Lee,K. H.; Won,H. J.; Kim,S. N.; Yoo,S. H.; Shin,I. S.; Shin,K. H.; Hong,S. H.; Lee,S. H.; Min,H. K.; Park,S. N.; Hur,S. J.
In Korea, antivenoms for the treatment of patients bitten by venomous snakes have been imported from Japan or China. Although there is cross-reactivity between these antibodies and venoms from snakes indigenous to Korea (e.g. Agkistrodon genus), protection is not optimal. Antivenoms specifically prepared to neutralize Korean snake venoms could be more effective, with fewer side effects. To this end, we established an infrastructure to develop national standards and created a standardized method to evaluate the efficacy of two horse-derived antivenoms using mouse lethal toxin test. Additionally, we determined the antivenoms neutralizing activity against lethal doses (LD50) of Agkistrodon halys (from Japan) and Jiangzhe Agkistrodon halys (from China) venoms....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-lethal toxin potency; Jiangzhe Angkistrodon halys; Lethal activity; Probit analysis; Slope.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400004

Purification and characterization of a hyaluronidase from venom of the spider Vitalius dubius (Araneae, Theraphosidae)
Provedor de dados: 80
Autores: Sutti,Rafael; Tamascia,Mariana Leite; Hyslop,Stephen; Rocha-e-Silva,Thomaz Augusto Alves.
BackgroundVenom hyaluronidase (Hyase) contributes to the diffusion of venom from the inoculation site. In this work, we purified and characterized Hyase from the venom of Vitalius dubius (Araneae, Theraphosidae), a large theraphosid found in southeastern Brazil. Venom obtained by electrical stimulation of adult male and female V. dubius was initially fractionated by gel filtration on a Superdex® 75 column. Active fractions were pooled and applied to a heparin-sepharose affinity column. The proteins were eluted with a linear NaCl gradient.ResultsActive fractions were pooled and assessed for purity by SDS-PAGE and RP-HPLC. The physicochemical tests included optimum pH, heat stability, presence of isoforms, neutralization by flavonoids and assessment of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyaluronidase; Venom; Purification; Vitalius dubius.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200312

Zoonoses and human beings
Provedor de dados: 80
Autores: Langoni,Hélio.
Tipo: Info:eu-repo/semantics/article
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200001

Systemic effects induced by intralesional injection of ω-conotoxin MVIIC after spinal cord injury in rats
Provedor de dados: 80
Autores: Oliveira,Karen M; Silva,Carla Maria O; Lavor,Mário Sérgio L; Rosado,Isabel R; Fukushima,Fabíola B; Assumpção,Anna Luiza FV; Neves,Saira MN; Motta,Guilherme R; Garcia,Fernanda F; Gomez,Marcus Vinícius; Melo,Marília M; Melo,Eliane G.
Background:Calcium channel blockers such as conotoxins have shown a great potential to reduce brain and spinal cord injury. MVIIC neuroprotective effects analyzed in in vitromodels of brain and spinal cord ischemia suggest a potential role of this toxin in preventing injury after spinal cord trauma. However, previous clinical studies with MVIIC demonstrated that clinical side effects might limit the usefulness of this drug and there is no research on its systemic effects. Therefore, the present study aimed to investigate the potential toxic effects of MVIIC on organs and to evaluate clinical and blood profiles of rats submitted to spinal cord injury and treated with this marine toxin. Rats were treated with placebo or MVIIC (at doses of 15, 30, 60 or 120...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conus magus; Cone snail; Histopathology; Hematology; Biochemistry.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200311

Genotyping of Clostridium perfringens associated with sudden death in cattle
Provedor de dados: 80
Autores: Miyashiro,S; Baldassi,L; Nassar,AFC.
Toxigenic types of Clostridium perfringens are significant causative agents of enteric disease in domestic animals, although type E is presumably rare, appearing as an uncommon cause of enterotoxemia of lambs, calves and rabbits. We report herein the typing of 23 C. perfringens strains, by the polymerase chain reaction (PCR) technique, isolated from small intestine samples of bovines that have died suddenly, after manifesting or not enteric or neurological disorders. Two strains (8.7%) were identified as type E, two (8.7%) as type D and the remainder as type A (82.6%). Commercial toxoids available in Brazil have no label claims for efficacy against type E-associated enteritis; however, the present study shows the occurrence of this infection. Furthermore,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Iota toxin; Sudden death; PCR; Cattle.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300010

Cloning of a novel acidic phospholipase A2 from the venom gland of Crotalus durissus cascavella (Brazilian northeastern rattlesnake)
Provedor de dados: 80
Autores: Guarnieri,MC; Melo,ESL; Melo,KMS; Albuquerque-Modesto,JC; Prieto-da-Silva,ARB; Rádis-Baptista,G.
The phospholipase A2 superfamily encompasses 15 groups that are classified into: secreted PLA2 (sPLA2); cytosolic PLA2 (cPLA2); Ca2+-independent intracellular PLA2 (iPLA2); platelet-activating factor acetylhydrolase (PAF-AH); and lysosomal PLA2. Currently, approximately 700 PLA2 sequences are known, of which 200 are obtained from the venom gland of Crotalinae snakes. However, thus far, little information is available on cloning, purification and structural characterization of PLA2 from Crotalus durisssus cascavela venom gland. In the present work, we report the molecular cloning of a novel svPLA2 from C. d. cascavella (Cdc), a predominant rattlesnake subspecies in northeastern Brazil. The Cdc svPLA2 cDNA precursor is 689 nucleotides long and encodes a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular toxinology; Crotalus durissus cascavella; Snake venom gland; CDNA library; Acidic PLA2.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400012

Bacillus subtilis induces morphological changes in Fonsecaea pedrosoi in vitro resulting in more resistant fungal forms in vivo
Provedor de dados: 80
Autores: Machado,AP; Anzai,M; Fischman,O.
Interactions among microorganisms may be the cause of morphological modifications, particularly in fungal cells. The aim of this work was to examine the changes that occur in cells of the fungus Fonsecaea pedrosoi after in vitro co-culturing with Bacillus subtilis and to explore the results of this interaction in vivo in an experimental murine infection. B. subtilis strain was inoculated into a 15-day pure culture of F. pedrosoi. In vitro, after 48 hours of co-culturing, the fungal cells were roundish. The secretion of fungal dark pigments and production of terminal chlamydoconidia were observed in hyphae after one week. In the in vivo study, two animal groups of 30 BALB/c mice each were employed. One group was inoculated intraperitoneally with hyphal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus subtilis; Fonsecaea pedrosoi; Microbial interaction; Co-culture; Fungal antagonism.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400009

Venomous snakebites and antivenom treatment according to a protocol for pediatric patients in taiwan
Provedor de dados: 80
Autores: Wang,JD; Tsan,YT; Yan-Chiao,Mao; Wang,LM.
Children bitten by venomous snakes comprise emergency cases that present high morbidity and mortality, especially in some regions of Asia and Africa. Reports on clinical features and management of envenomated children are scarce. This observational study implemented a protocol for antivenom use in pediatric snakebite victims in Taiwan, between 1994 and 2007. The present work investigated demographics, clinical features and effects of antivenom therapy and the complications and prognosis for snakebites. A total of 55 children and adolescents, with a median age of 9.9 years (ranging from 2 to 18 years), was identified. Forty-five patients (82%) were bitten between May and September. Thirty-five patients (64%) received antivenom therapy, 28 of them (80%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snakebites; Children; Antivenom.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400006

Isolation of Staphylococcus epidermidis from inflamed upper respiratory tract of an orange-spined hairy dwarf porcupine (Sphiggurus villosus)
Provedor de dados: 80
Autores: Fornazari,F; Guimarães,FF; Teixeira,CR; Langoni,H.
The orange-spined hairy dwarf porcupine (Sphiggurus villosus) is a rodent species common in most parts of South America, and little is known about the pathologies that can afflict it. A specimen was delivered at the Wildlife Research and Medical Center (CEMPAS), School of Veterinary Medicine and Animal Husbandry, UNESP, Botucatu, SP, Brazil. The animal showed intense apathy, with purulent secretion in the nasal cavity and fracture of the lumbar spine. Due to the unfavorable prognosis, the porcupine was euthanized and microbiological culture of nasal discharge showed Staphylococcus epidermidis. The antimicrobial resistance test revealed sensitivity to all tested antimicrobials (ampicillin, oxacillin, tetracycline, penicillin G, neomycin, cephalexin,...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Staphylococcus epidermidis; Orange-spined hairy dwarf porcupine; Sphiggurus villosus; Antimicrobials; Susceptibility.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400016

The evolution and distribution of noxious species of scorpions (Arachnida: Scorpiones)
Provedor de dados: 80
Autores: Lourenço,Wilson R.
Abstract This contribution attempts to bring some general information on the evolution and, in particular, on the geographic distribution of scorpion species noxious to humans. Since 95% of the scorpions incidents are generated by specimens of the family Buthidae C. L. Koch, the analysis will be limited to this familial group. As in previous similar contributions, the content of this work is mostly addressed to non-specialists whose research embraces scorpions in several fields such as venom toxins and public health. Only in recent years, efforts have been made to create better links between ‘academic scorpion experts’ and other academic non-specialists who use scorpions in their research. Even if a larger progress can yet be expected from such exchanges,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion; Noxious species; Patterns of distribution; Biased results; Buthidae.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100201

Analgesic and side effects of intravenous recombinant Phα1β
Provedor de dados: 80
Autores: Rigo,Flavia Karine; Rossato,Mateus Fortes; Borges,Vanessa; Silva,Juliana Figueira da; Pereira,Elizete Maria Rita; Ávila,Ricardo Andrez Machado de; Trevisan,Gabriela; Santos,Duana Carvalho dos; Diniz,Danuza Montijo; Silva,Marco Aurélio Romano; Castro Junior,Célio José de; Cunha,Thiago Mattar; Ferreira,Juliano; Gomez,Marcus Vinicius.
ABSTRACT  Background: Intrathecal injection of voltage-sensitive calcium channel blocker peptide toxins exerts analgesic effect in several animal models of pain. Upon intrathecal administration, recombinant Phα1β exerts the same analgesic effects as the those of the native toxin. However, from a clinical perspective, the intrathecal administration limits the use of anesthetic drugs in patients. Therefore, this study aimed to investigate the possible antinociceptive effect of intravenous recombinant Phα1β in rat models of neuropathic pain, as well as its side effects on motor, cardiac (heart rate and blood pressure), and biochemical parameters.  Methods: Male Wistar rats and male Balb-C mice were used in this study. Giotto Biotech® synthesized the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recombinant Phα1β Analgesia Neuropathic pain Intravenous drug delivery system Side effects; Cardiac function; Motor activity; Biochemicals.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100309

A C-type lectin from Bothrops jararacussu venom can adhere to extracellular matrix proteins and induce the rolling of leukocytes
Provedor de dados: 80
Autores: Elífio-Esposito,S. L.; Hess,P. L.; Moreno,A. N.; Lopes-Ferreira,M.; Ricart,C. A. O.; Souza,M. V.; Hasselman-Zielinski,F.; Becker,J. A.; Pereira,L. F.
Purification of a lectin from Bothrops jararacussu venom (BjcuL) was carried out using agarose-D-galactose affinity gel. MALDI-TOF gave a major signal at m/z 32028, suggesting the presence of a dimmer composed of two identical subunits. Divalent cations were required for the lectin activity, as complete absence of such ions reduced hemagglutination. BjcuL was more effective at neutral pH and showed total loss of activity at pH values below 4.0 and above 9.0. Its agglutinating activity remained stable at 25°C until 60min, but increased when at 35°C for at least 15min. Adhesion assays to extracellular matrix (ECM) glycoproteins showed that the biotinylated lectin (0.039-5.0µg/100µl) was capable of binding to fibronectin and vitronectin in a dose-dependent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venoms; Fibronectins; Vitronectin; Snakes; Intravital microscopy; Leukocytes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400009

Neural network-based species identification in venom-interacted cases in India
Provedor de dados: 80
Autores: Maheshwari,R.; Kumar,V.; Verma,H. K.
India is home to a number of venomous species. Every year in harvesting season, a large number of productive citizens are envenomed by such species. For efficient medical management of the victims, identification of the aggressor species as well as assessment of the envenomation degree is necessary. Species identification is generally based on the visual description by the victim or a witness and is therefore quite likely to be erroneous. Symptomatic identification remains the only available method. In a previous published work, the authors proposed a classification table for snake species based on manifested symptoms applicable in Indian subcontinent. The classification table serves the purpose to a great deal but as a manual method it demands human...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bites and stings; Symptoms; Species identification; Neural network.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400008

Hemolytic activity of venom from crown-of-thorns starfish Acanthaster planci spines
Provedor de dados: 80
Autores: Lee,Chi-Chiu; Tsai,Wann-Sheng; Hsieh,Hernyi Justin; Hwang,Deng-Fwu.
Background : The crown-of-thorns starfish Acanthaster planci is a venomous species from Taiwan whose venom provokes strong hemolytic activity. To understand the hemolytic properties of A. planci venom, samples were collected from A. planci spines in the Penghu Islands, dialyzed with distilled water, and lyophilized into A. planci spine venom (ASV) powder. Results : Both crude venom and ASV cause 50% hemolysis at a concentration of 20 μg/mL. The highest hemolytic activity of ASV was measured at pH 7.0-7.4; ASV-dependent hemolysis was sharply reduced when the pH was lower than 3 or greater than 8. There was almost no hemolytic activity when the Cu2+ concentration was increased to 10 mM. Furthermore, incubation at 100°C for 30 to 60 minutes sharply decreased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crown-of-thorns starfish; Spine; Venoms; Hemolysis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100312

Anti-inflammatory activity of Blutaparon portulacoides ethanolic extract against the inflammatory reaction induced by Bothrops jararacussu venom and isolated myotoxins BthTX-I and II
Provedor de dados: 80
Autores: Pereira,IC; Barbosa,AM; Salvador,MJ; Soares,AM; Ribeiro,W; Cogo,JC; Zamuner,SR.
This article reports the anti-inflammatory effect of Blutaparon portulacoides (B. portulacoides), specifically the ethanolic extract of its aerial parts, on the edema formation and leukocyte influx caused by Bothrops jararacussu (B. jararacussu) snake venom and Bothropstoxin-I and II (BthTX-I and II) isolated from this venom as an alternative treatment for Bothrops snakebites. The anti-inflammatory effect of B. portulacoides ethanolic extract was compared with an animal group pretreated with dexamethasone. B. portulacoides ethanolic extract significantly inhibited paw edema induced by B. jararacussu venom and by BthTX-I and II. Also, results demonstrated that the extract caused a reduction of the leukocyte influx induced by BthTX-I. However, the extract...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararacussu; Myotoxins; Inflammation; Edema; Blutaparon portulacoides.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300013

Alpha-type phospholipase A2 inhibitors from snake blood
Provedor de dados: 80
Autores: Santos-Filho,Norival A.; Santos,Claudia T.
Abstract It is of popular and scientific knowledge that toxins from snake venom (among them the PLA2 and myotoxins) are neutralized by various compounds, such as antibodies and proteins purified from animal blood. Venomous and nonvenomous snakes have PLA2 inhibitory proteins, called PLIs, in their blood serum. One hypothesis that could explain the presence of these PLIs in the serum of venomous snakes would be self-protection against the enzymes of their own venom, which eventually could reach the circulatory system. However, the presence of PLIs in non-venomous snakes suggests that their physiological role might not be restricted to protection against PLA2 toxins, but could be extended to other functions, as in the innate immune system and local...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phospholipases A2; Myotoxin; Myotoxin inhibitor; ΑPLI; Snake blood.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100203

Bioactivities of extracts from the marine sponge Halichondria panicea
Provedor de dados: 80
Autores: Purushottama,GB; Venkateshvaran,K; Pani Prasad,K; Nalini,P.
In the present study, we screened the biological activity of extracts from the marine sponge Halichondria panicea collected in the Arabian Sea. Crude toxin was obtained by methanol, chloroform-methanol (2:1) and aqueous extraction. Subsequently, the protein concentration of each crude extract was determined. The impact of both sponge methanolic and aqueous extracts was found to increase activities of Na+-K+ ATP-ase and Mg++ ATP-ase. In the case of chloroform-methanol extract, higher concentrations increased acetylcholine esterase (AchE) activity. The methanolic and chloroform-methanol extracts exhibited hemolytic activity on chicken and human erythrocytes, whereas the aqueous extract failed to do so. Methanol and aqueous extracts produced an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Halichondria panicea; Phagocytosis; Neuromodulation; Hemolysis; Angiogenesis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300007

CEVAP journal towards a new phase
Provedor de dados: 80
Autores: Barraviera,Benedito.
Tipo: Info:eu-repo/semantics/other
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100001

Biochemical and toxinological characterization of Naja sumatrana (Equatorial spitting cobra) venom
Provedor de dados: 80
Autores: Yap,MKK; Tan,NH; Fung,SY.
The lethal and enzymatic activities of venom from Naja sumatrana (Equatorial spitting cobra) were determined and compared to venoms from three other Southeast Asian cobras (Naja sputatrix, Naja siamensis and Naja kaouthia). All four venoms exhibited the common characteristic enzymatic activities of Asiatic cobra venoms: low protease, phosphodiesterase, alkaline phosphomonoesterase and L-amino acid oxidase activities, moderately high acetylcholinesterase and hyaluronidase activities and high phospholipase A2. Fractionation of N. sumatrana venom by Resource® S cation exchange chromatography (GE Healthcare, USA) yielded nine major protein peaks, with all except the acidic protein peak being lethal to mice. Most of the protein peaks exhibit enzymatic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Naja; Cobra venom; Enzyme activity; Cardiotoxin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400012

Intraspecific variation of Bothrops pubescens (cope, 1869) venom in Uruguay (serpentes: viperidae)
Provedor de dados: 80
Autores: Morais,V.; Baraibar,M.; Carreira,S.
In Uruguay, there was no information about the variations degree in Bothrops pubescens venoms until the present work, in which we investigated intraspecific venom variation using polyacrylamide gel electrophoresis (SDS-PAGE). We found some differences in the venom protein profile; however, they were not related to the parameters studied (geographic distribution, weight, sex, and captivity time). Moreover, we distinguished two different groups in relation to band densities at 49 and 57 kDa. Specimens with predominant density in the 49kDa band tend to be predominantly females. Weight distribution in this group extended for all the range (150-1500 g) with an average weight of 720 g. The other group (57kDa predominant band) showed restricted weight range...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venom; Bothrops pubescens; Uruguay; Antivenom production; Electrophoretic pattern.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400007

Serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom components in a rabbit
Provedor de dados: 80
Autores: Tan,NH; Ponnudurai,G; Fung,SY.
The serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom - specifically two of its components, the major hemorrhagin (rhodostoxin) and a thrombin-like enzyme - was examined in a rabbit by double-sandwich enzyme-linked immunosorbent assay (ELISA). The animal received intramuscularly a 1.0-mg/kg dose of C. rhodostoma venom. The venom level in serum peaked 12 hours after the injection, followed by a gradual decline and finally reached low rates 72 hours after administration. The serum kinetic profile of venom components, however, did not correspond to the profile of the whole C. rhodostoma venom. The serum levels of the C. rhodostoma thrombin-like enzyme increased slowly and peaked only 48 hours post-injection. Then both thrombin-like enzyme...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calloselasma rhodostoma venom; Thrombin-like enzyme; Hemorrhagin; Serum kinetics; Rabbit; ELISA.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200014

Retrospective study on the incidence of envenomation and accessibility to antivenom in Burkina Faso
Provedor de dados: 80
Autores: Gampini,Sandrine; Nassouri,Sonia; and,Jean-Philippe Chippaux; Semde,Rasmané.
Abstract  Background  Snakebite is a common neglected public health issue, especially in poor rural areas of sub-Saharan Africa, Asia and Latin America. Passive immunotherapy with safe and effective antivenom is the only approved treatment for it. This study aimed to determine the incidence of snakebites, and to assess the availability and accessibility of antivenoms, from 2010 to 2014, in Burkina Faso.  Methods  The assessment of snakebite cases managed in all health facilities from 2010 to 2014 was performed from the Statistical Yearbook of the Ministry of Health. Antivenom consumption data were collected from the drug wholesalers established in Burkina Faso.  Results  Snakebites are among the five leading causes of consultations in health districts....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Envenomation; Snakebite; Antivenom; Burkina Faso.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100309

Evolution and prophylaxis of human rabies
Provedor de dados: 80
Autores: Ayres,J.A.; Peraçoli,M. T. S.; Barraviera,B.
Rabies is a viral acute encephalitis of progressive and fatal outcome, particular of hot-blooded animals, and accidentally affecting men. Since it is a zoonosis with different animal species acting as a reservoir in the nature, this disease is a great public health problem in several countries in development. Prophylactic treatments for human rabies started in 1885 with Louis Pasteur, and developed in order to provide higher protection and lower incidence of side effects. Today, treatments of pre and post-exposure to the virus are well established, with excellent results of protection for individuals exposed to animals potentially contaminated by the rabies virus. These treatments consist of utilising the vaccine isolatedly or in combination with equine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human rabies; Prophylaxis; Heterologous serum; Vaccine.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100003

Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
Provedor de dados: 80
Autores: Carnieli JR,P.; Ventura,A. M.; Durigon,E. L.
A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested. The presence of the pSH-G plasmid was confirmed by means of polymerase chain reaction (PCR) after each of ten cell passages, and the results were positive. The stable BHK-21/pSH-G+ clone obtained can be used in the study of rabies as well as in the production of vaccines.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; G gene; Plasmids; Transfection.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400015

Second record of Tityus bahiensis (Scorpiones, Buthidae) from Venezuela: epidemiological implications
Provedor de dados: 80
Autores: De Sousa,L.; Borges,A.; Manzanilla,J.; Biondi,I.; Avellaneda,E.
This work reports the second record of the scorpion Tityus bahiensis Perty from Venezuela. The specimen was found alive in a wardrobe at a hotel resort in Margarita Island, northeastern Venezuela. Morphological characterization allowed its assignment to the Tityus bahiensis population inhabiting the southernmost area of the species' geographic range, e.g. the state of São Paulo in Brazil, northern Argentina and Paraguay. The fact that the only available Venezuelan antiscorpion (anti-Tityus discrepans) serum does not neutralize the effects of alpha- and beta-toxin from Tityus serrulatus venom (which resembles in composition that of T. bahiensis) constitutes a warning to local clinicians confronted with envenomations by noxious species transported to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tityus; Scorpiones; Buthidae; Exotic species.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100015

Heterologous expression, protein folding and antibody recognition of a neurotoxin from the Mexican coral snake Micrurus laticorallis
Provedor de dados: 80
Autores: Clement,Herlinda; Flores,Vianey; la Rosa,Guillermo De; Zamudio,Fernando; Alagon,Alejandro; Corzo,Gerardo.
Abstract  Background  The cysteine-rich neurotoxins from elapid venoms are primarily responsible for human and animal envenomation; however, their low concentration in the venom may hamper the production of efficient elapid antivenoms. Therefore, the aim of the present study was to produce fully active elapid neurotoxic immunogens for elapid antivenom production.  Method  Cysteine-rich neurotoxins showed recombinant expression in two strains of E. coli, and were purified using affinity chromatography and reverse-phase HPLC (rpHPLC).  Results  The cDNA of the four disulfide-bridged peptide neurotoxin Mlat1 was cloned into a modified expression vector, pQE30, which was transfected into two different E. coli strains. The recombinant toxin (HisrMlat1) was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micrurus laticorallis; Protein folding; Recombinant; Elapid; Toxin; Protein recognition.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100318

Brazilian spotted fever: a reemergent zoonosis
Provedor de dados: 80
Autores: Greca,H.; Langoni,H.; Souza,L.C.
Brazilian spotted fever is caused by the bacterium Rickettsia rickettsii, which is the most pathogenic species of the spotted-fever rickettsiae group and is transmitted by the bite of infected ticks. Amblyomma cajennense is the most important tick species involved in the cycle of this zoonosis in Brazil as it presents low host specificity, great number of natural reservoirs and wide geographic distribution. It was first described in the state of São Paulo in 1929 and later in Rio de Janeiro, Minas Gerais and Bahia. The number of cases decreased in the 1940's with the development of new plague control techniques and antibiotics. In the last decades, the number of new cases has increased. The current review aimed at reporting some of the epidemiological and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian spotted fever; Rickettsia rickettsii; Amblyomma cajennense.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100002

Construction of ethics in clinical research: clinical trials registration
Provedor de dados: 80
Autores: Caramori,C. A.
Scientific development that has been achieved through decades finds in clinical research a great possibility of translating findings to human health application. Evidence given by clinical trials allows everyone to have access to the best health services. However, the millionaire world of pharmaceutical industries has stained clinical research with doubt and improbability. Study results (fruits of controlled clinical trials) and scientific publications (selective, manipulated and with wrong conclusions) led to an inappropriate clinical practice, favoring the involved economic aspect. In 2005, the International Committee of Medical Journal Editors (ICMJE), supported by the World Association of Medical Editors, started demanding as a requisite for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomedical research; Clinical trials; Ethics; Drug information services; World Health Organization.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400004

Exposure to 50 Hz electromagnetic field changes the efficiency of the scorpion alpha toxin
Provedor de dados: 80
Autores: Jankowska,Milena; Pawlowska-Mainville,Agnieszka; Stankiewicz,Maria; Rogalska,Justyna; Wyszkowska,Joanna.
AbstractBackground Extremely low-frequency (50 Hz) electromagnetic field (ELF-EMF) is produced by electric power transmission lines and electronic devices of everyday use. Some phenomena are proposed as “first effects” of ELF-EMF: the discrete changes in the membrane potential and the increase of the calcium channel activity as well as the intracellular concentration of Ca 2+ . Interaction of the scorpion alpha toxin with the sodium channel depends on the orientation of the charges and may be perturbed by changes in the membrane polarization. The toxin induces overexcitability in the nervous system and an increase in the neurotransmitters released with different consequences, mainly the paralysis of muscles. We assumed that the exposure to ELF-EMF 0.7 mT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 50 Hz electromagnetic field; Alpha scorpion toxin; Cockroach; Bioelectrical activity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100344

Snakebites in southwestern Goiás State, Brazil
Provedor de dados: 80
Autores: Pacheco,U. P.; Zortéa,M.
The present study evaluated snakebite cases recorded by the Southwest II Regional Health Bureau [Regional de Saúde Sudoeste II], Goiás State, Brazil. The following data were analyzed: accident period; patient's age and gender; bite site; envenomation severity; and time elapsed between the bite and medical treatment. We evaluated 211 cases recorded between January 2002 and December 2005, which resulted in an incidence coefficient of 32.4/100,000 inhabitants. Most accidents were due to the bite of snakes from the Bothrops genus (78.2%). The months between January and April had a larger number of accidents (93%-44.1%), although no significant differences were observed. Only one death occurred, resulting in a mortality rate of 0.5%. The lower limbs were the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake bites; Cerrado; Jataí; Goiás.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100011

Methods in toxinology: snake toxins
Provedor de dados: 80
Tipo: Info:eu-repo/semantics/article
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300013

Molecular typing of Mycobacterium tuberculosis strains isolated from serum positive and negative HIV patients in the Bauru region - São Paulo
Provedor de dados: 80
Autores: Baptista,I. M. F. D.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100018

Identification, expression and characterization of the recombinant Sol g 4.1 protein from the venom of the tropical fire ant Solenopsis geminata
Provedor de dados: 80
Autores: Srisong,Hathairat; Sukprasert,Sophida; Klaynongsruang,Sompong; Daduang,Jureerut; Daduang,Sakda.
Abstract  Background: Fire ant venom is a complex mixture consisting of basic piperidine alkaloids, various biologically active peptides and protein components, including a variety of major allergenic proteins. Tropical fire ant Solenopsis geminata is an important stinging ant species that causes anaphylaxis and serious medical problems. Although the biological activities of allergenic venom proteins that are unique to ant venom, particularly Solenopsis 2 and 4, are still unknown, these proteins are believed to play important roles in mediating the effects of the piperidine derivatives in the venom.  Methods: In the present study, the cDNA cloning, sequencing and three-dimensional structure of Sol g 4.1 venom protein are described. The recombinant Sol g...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fire ant; Sol g 4.1 protein; Allergen; Venom protein; Stinging ant.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100314

Presumptive thrombotic thrombocytopenic purpura following a hump-nosed viper (Hypnale hypnale) bite: a case report
Provedor de dados: 80
Autores: Withana,Milinda; Rodrigo,Chaturaka; Gnanathasan,Ariaranee; Gooneratne,Lallindra.
Hump-nosed viper bites are frequent in southern India and Sri Lanka. However, the published literature on this snakebite is limited and its venom composition is not well characterized. In this case, we report a patient with thrombotic thrombocytopenic purpura-like syndrome following envenoming which, to the best of our knowledge, has not been reported in the literature before. A 55-year-old woman from southern Sri Lanka presented to the local hospital 12 hours after a hump-nosed viper (Hypnale hypnale) bite. Five days later, she developed a syndrome that was characteristic of thrombotic thrombocytopenic purpura with fever, thrombocytopenia, microangiopathic hemolysis, renal impairment and neurological dysfunction in the form of confusion and coma. Her...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Thrombotic thrombocytopenic purpura; Hump-nosed viper; Thrombotic microangiopathy; Snakebite; Hemolytic uremic syndrome.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200504

Optimization of antiscorpion venom production
Provedor de dados: 80
Autores: Ozkan,O.; Adigüzel,S.; Ates,C.; Bozyigit,I.; Filazi,A.
The present study was carried out to produce highly efficient antivenom from a small number of telsons in a short time. Venom solution was prepared through maceration of telsons from Androctonus crassicauda (Olivier, 1807) collected in the Southeastern Anatolia Region, Turkey. Lethal dose 50% (LD50) of the venom solution injected into mice was 1 ml/kg (95% confidence interval; 0.8-1.3), according to probit analysis. Different adjuvants (Freund's Complete Adjuvant, Freund's Incomplete Adjuvant, and 0.4% aluminium phosphate), at increasing doses and combined with venom, were subcutaneously injected into horses on days 0, 14, 21, 28, 35, and 42 of the experiment. Antivenom was collected from the immunized horses on days 45, 48, and 51 using the pepsin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion; Antivenom; Androctonus crassicauda.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300004

Preliminary assessment of Hedychium coronarium essential oil on fibrinogenolytic and coagulant activity induced by Bothrops and Lachesis snake venoms
Provedor de dados: 80
Autores: Miranda,Cíntia A SF; Cardoso,Maria G; Mansanares,Mariana E; Gomes,Marcos S; Marcussi,Silvana.
Background The search for new inhibitors of snake venom toxins is essential to complement or even replace traditional antivenom therapy, especially in relation to compounds that neutralize the local effects of envenomations. Besides their possible use as alternative to traditional antivenom therapy, some plant species possess bioactive secondary metabolites including essential oils, which can be extracted from weeds that are considered substantial problems for agriculture, such as Hedychium coronarium.Methods The essential oils of leaves and rhizomes from H. coronarium were extracted by hydrodistillation, and their potential inhibitory effects on the coagulant and fibrinogenolytic activities induced by the venoms of Lachesis muta,Bothrops atrox and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Volatile oils; Weeds; Natural inhibitors; Antivenom therapy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200313

Cell migration induced by Leishmania (Leishmania) amazonensis, Leishmania (Leishmania) major and Leishmania (Viannia) braziliensis into the peritoneal cavity of BALB/c mice
Provedor de dados: 80
Autores: Wakimoto,DT; Gaspareto,KV; Silveira,TGV; Lonardoni,MVC; Aristides,SMA.
In American cutaneous leishmaniasis, the initial infection phase is characterized by recruitment of neutrophils and monocytes. The migration of these cells in response to the presence of Leishmania in the peritoneum of affected animals remains unclear. The objective of this study was to investigate cell migration to the peritoneum of BALB/c mice after infection with Leishmania (Leishmania) amazonensis, Leishmania (Viannia) braziliensis and Leishmania (Leishmania) major. Initially, Leishmania spp. was intraperitoneally inoculated in five groups of six animals each and the cell migration was analyzed 0, 3, 6, 12, 24 and 48 hours after infection. Different cell counts were performed with a staining kit and showed a higher percentage of polymorphonuclear than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BALB/c mice; Leishmania spp.; American cutaneous leishmaniasis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100017

Acute hepatotoxicity of Crotalus durissus terrificus (South American rattlesnake) venom in rats
Provedor de dados: 80
Autores: França,RF; Vieira,RP; Ferrari,EF; Souza,RA; Osorio,RAL; Prianti-Jr,ACG; Hyslop,S; Zamuner,SR; Cogo,JC; Ribeiro,W.
Venom of the South American rattlesnake, Crotalus durissus terrificus (Cdt), presents myotoxic and neurotoxic outcomes, but reports on its effects on the liver are scarce. This study examined the hepatotoxicity resulting from Cdt venom administration (100, 200 and 300 µg/kg) in male Wistar rats. Animals were studies at 3, 6, 9 and 12 hours after venom injection. The hepatotoxicity was assessed through serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (AP), gamma glutamyl transferase (GGT), bilirrubin and also by histopathological evaluation. All the different concentrations of Cdt venom resulted in increased levels of hepatic enzymes, when compared with the control group, except for the 100 µg/kg dose,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durissus terrificus; Hepatotoxicity; Histology; AST; ALT; Rattlesnake; Liver enzymes; Venom.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100007

Kinetic investigations and stability studies of two Bothrops L-amino acid oxidases
Provedor de dados: 80
Autores: Costa,Tássia R.; Carone,Sante E. I.; Tucci,Luiz F. F.; Menaldo,Danilo L.; Rosa-Garzon,Nathalia G.; Cabral,Hamilton; Sampaio,Suely V.
Abstract  Background: L-amino acid oxidases isolated from snake venoms (SV-LAAOs) are enzymes that have great therapeutic potential and are currently being investigated as tools for developing new strategies to treat various diseases, including cancer and bacterial infections. The main objective of this study was to make a brief evaluation of the enzymatic stability of two Bothrops LAAOs, one isolated from Bothrops jararacussu (BjussuLAAO-II) and the other from Bothrops moojeni (BmooLAAO-I) venoms.  Methods and results: The enzymatic activity and stability of both LAAOs were evaluated by microplate colorimetric assays, for which BjussuLAAO-II and BmooLAAO-I were incubated with different L-amino acid substrates, in the presence of different ions, and at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops; L-amino acid oxidase; Enzymatic stability.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100327

Effect of environmental mycobacteria (Mycobacterium avium) on immunity induced by a DNA vaccine (DNAhsp65) against tuberculosis
Provedor de dados: 80
Autores: Martins,Douglas Rodrigues.
Tipo: Info:eu-repo/semantics/other
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400013

A study on the genetic diversity of Androctonus crassicauda (Olivier, 1807; Scorpiones: Buthidae) from Turkey
Provedor de dados: 80
Autores: Ozkan,O; Ahmet,C; Zafer,K.
Mitochondrial DNA (mtDNA) has been widely employed in phylogeographic and phylogenetic studies. In the present study, the genetic identification of the scorpion Androctonus crassicauda (Olivier, 1807) was carried out by using the 16S mitochondrial gene, since this scorpion represents the most important species in Turkey regarding scorpionism and antivenom production. Two genetic groups were found according to the sequence analysis results, while five different loci at the nucleotide level presented genetic variation in the 16S region when compared to a known A. crassicauda sequence data (GenBank, AJ277598). Nucleotide variations found in the current work constitute the first descriptive report for A. crassicauda. Moreover, future studies may enlighten the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion; Androctonus crassicauda; PCR; Genotype; Turkey.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400010

Toxicity and symptomatic identification of species involved in snakebites in the Indian subcontinent
Provedor de dados: 80
Autores: Kumar,V.; Maheshwari,R.; Verma,H. K.
Snakebites, being the major occupational hazard for farm workers, claim a large number of lives in the Indian subcontinent. During the course of medical management, identification of the biting species is given a low priority, resorting to prescription of polyvalent anti-snake venom. Whereas the World Health Organization (WHO) recommends monospecific anti-snake venom instead of polyvalent anti-snake venom. Thus, it is essential to identify the aggressor species either by a visual inspection or by the symptoms of the victim. Along with the four deadly venomous species (cobra, krait, Russell's viper, and saw-scaled viper), there are a number of other species of medical importance, whose venoms and bites have not been paid much attention. Thus, a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aglyphs; Envenomation; Opistoglyphs; Proteroglyphs; Snakebite; Species-specific toxicity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100002

Mesobuthus eupeus scorpionism in Sanliurfa region of Turkey
Provedor de dados: 80
Autores: Ozkan,O.; Kat,I.
The epidemiology and clinical findings of scorpion stings in Sanliurfa region of Turkey, from May to September 2003, were evaluated in this study. Mesobuthus eupeus (M. eupeus) plays a role on 25.8% of the scorpionism cases. This study also showed that intoxications caused by M. eupeus in the southeast of Anatolia region were seen in hot months of the summer, especially on July. Females and people above 15 years old were mostly affected and stung on extremities. Intense pain in the affected area was observed in 98.7% cases, hyperemia in 88.8%, swelling in 54.6%, burning in 19.7%, while numbness and itching were seen less frequently. In our study, the six most frequently observed symptoms were local pain, hyperemia, swelling, burning, dry mouth, thirst,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesobuthus eupeus; Turkey; Scorpionism; Epidemiology; Clinical symptoms.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400008

Distribution and factors associated with Fasciola hepatica infection in cattle in the south of Espírito Santo State, Brazil
Provedor de dados: 80
Autores: DP,Alves; MB,Carneiro; IVF,Martins; CC,Bernardo; DM,Donatele; Pereira Júnior OS,; BR,Almeida; BR,Avelar; AGC,Leão.
Fasciolosis is a disease that affects the liver parenchyma and bile ducts of numerous animals, including humans, which causes economic losses and threatens public health. The present work aimed at reporting the distribution and factors connected with Fasciola hepatica infection in cattle in ten municipalities in southern Espírito Santo, Brazil, between 2008 and 2009. During this period, 50 rural properties in ten cities were analyzed and fecal samples from 10% of the cattle on each farm were collected. Of the 717 samples analyzed, 154 (21.33%) presented Fasciola hepatica eggs. As for the 50 rural properties studied, 32 (64%) had animals positive for fasciolosis. Throughout the evaluation, it was observed that 42 (84%) rural properties had flooded areas, 33...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fasciolosis; Epidemiology; Ruminants.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300006

Copulation of Opisthogonimus fonsecai Ruiz &amp;amp; Leão, 1942 (Trematoda, Digenea, Plagiorchiidae), parasite of Bothrops moojeni Hoge, 1966 (Serpentes, Viperidae)
Provedor de dados: 80
Autores: Silva,R. J.
Copulation in Opisthogonimus fonsecai Ruiz &amp;amp; Leão, 1942 (Trematoda, Digenea, Plagiorchiidae), parasite of Bothrops moojeni Hoge, 1966 (Serpentes, Viperiade), was reported. Data on the histology of the cirrus and metraterm of the copulants were presented. Morphology of the cirrus was also examined by scanning electron microscopy.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copulation; Opisthogonimus fonsecai; Trematoda; Histology; SEM.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100008

Cloning, structural modelling and characterization of VesT2s, a wasp venom hyaluronidase (HAase) from Vespa tropica
Provedor de dados: 80
Autores: Rungsa,Prapenpuksiri; Incamnoi,Paroonkorn; Sukprasert,Sophida; Uawonggul,Nunthawun; Klaynongsruang,Sompong; Daduang,Jureerut; Patramanon,Rina; Roytrakul,Sittiruk; Daduang,Sakda.
Abstract  Background: Wasp venom is a complex mixture containing proteins, enzymes and small molecules, including some of the most dangerous allergens. The greater banded wasp (Vespa tropica) is well-known for its lethal venom, whose one of the major components is a hyaluronidase (HAase). It is believed that the high protein proportion and activity of this enzyme is responsible for the venom potency.  Methods: In the present study, cDNA cloning, sequencing and 3D-structure of Vespa tropica venom HAase were described. Anti-native HAase antibody was used for neutralization assay.  Results: Two isoforms, VesT2a and VesT2b, were classified as members of the glycosidase hydrolase 56 family with high similarity (42–97 %) to the allergen venom HAase. VesT2a gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wasp venom; Vespa tropica; Hyaluronidase (HAase).
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100321

Study of anticardiolipin antibody in hepatitis C virus-positive patients
Provedor de dados: 80
Autores: Elsayeh,H; Abdallah,N; Hamed,NA; Morsi,MG; Eldighidy,A; Kamal,HA.
Several antibodies, including anticardiolipin antibodies (ACA), have been detected among chronically infected hepatitis C virus (HCV) patients. The present work aimed at ascertaining the clinical significance of ACA levels among HCV infection associated with two commonly encountered diseases, thrombocytopenia and arteriovenous-shunt malfunction. Six groups were studied, 11 HCV-positive thrombocytopenic patients (group I), 14 HCV-positive non-thrombocytopenic patients (group II) and 15 healthy controls (group III), 11 anti-HCV-positive hemodialysis patients with non-functioning shunt (group IV), 14 anti-HCV-positive hemodialysis patients with patent shunt (group V) (Bain Medical Equipment Co., China) and 15 healthy controls (group VI). Anticardiolipin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C virus; Hemodialysis; Anticardiolipin antibody; Enzyme-linked immunosorbent assay.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400014

Relationship between Helicobacter pylori detection and an increased risk of infection in childhood
Provedor de dados: 80
Autores: Venerando,R; Rasmussen,LT; de Labio,RW; Gatti,LL; Francisco,O; Viani,GA; Rivera,LML; Payão,SLM.
The risk of developing gastric cancer is believed to be related to differences among Helicobacter pylori strains and the inflammatory responses mediated by host genetic factors. H. pylori infection is acquired at an early age and in the absence of appropriate antibiotic therapy, it generally persists for life. Tp53 gene regulates the transcription of several cytokines and chemokines involved in innate immunity and its action may be influenced by the presence of different H. pylori strains. The present study aimed to detect H. pylori in pediatric patients, to access Tp53 polymorphism at codon 72 and to correlate such findings with age and histopathological results. Three hundred and forty-two patients were analyzed. DNA from their gastric biopsies was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Childhood; Tp53 polymorphims; PCR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400004

The versatile strategies of Escherichia coli pathotypes: a mini review
Provedor de dados: 80
Autores: Sousa,C. P.
The widespread species Escherichia coli includes a broad variety of different types, ranging from highly pathogenic strains to avirulent isolates. Few microorganisms are as versatile as E. coli. Pathogenic strains remain a leading cause of severe and persistent infant diarrhea in developing countries. They may be limited to colonization of a mucosal surface or can disseminate throughout the body and have been implicated in urinary tract infection, sepsis/meningitis and gastrointestinal infection. The human gastrointestinal tract is susceptible to diarrheagenic E. coli infections. Escherichia coli have effectively managed to subvert the host cytoskeleton for their own purposes causing substantial diarrheal disease, a major public health problem worldwide....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pathogenicity; Virulence; Escherichia coli pathotypes; Versatile strategies; Diarrheagenic E. coli categories; Public health.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300002

Toxic thermoresistant metabolites of Fusarium oxysporum are capable of inducing histopathological alterations in Wistar rats
Provedor de dados: 80
Autores: Hernandes,L; Marangon,AV; Salci,T; Svidzinski,TIE.
The genus Fusarium is known to produce mycotoxins that cause fusariosis in plants, animals and humans. Mycotoxins are among the virulence factors of this genus. Metabolic extracts of Fusarium oxysporum, isolated from a patient with onychomycosis and sterilized by filtration or autoclave, were inoculated intradermally into Wistar rats at concentrations of 0.25, 0.5 and 1 µg/µL, and the effects on their tegument were observed at 24 and 72 hours. After histological procedures and staining by hematoxylineosin, the sections were studied for their inflammatory-reaction intensity and for evidence of injury and tissue distortion. Inflammatory reactions in the dermis and the subcutaneous tissue were observed at all concentrations of the inoculated extract tested....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fungal skin disease; Fusarium; Mycotoxins.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200003

XLVI Congress of the Brazilian Society of Tropical Medicine Foz do Iguaçu, Paraná State, Brazil - March 14th to 18th, 2010 central subject - environmental changes and Tropical diseases: challenges of the millennium
Provedor de dados: 80
Autores: Telles Filho,Flavio de Queiroz.
Tipo: Info:eu-repo/semantics/other
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400001

Heterologous expression and mutagenesis of recombinant Vespa affinis hyaluronidase protein (rVesA2)
Provedor de dados: 80
Autores: Rungsa,Prapenpuksiri; Janpan,Piyapon; Saengkun,Yutthakan; Jangpromma,Nisachon; Klaynongsruang,Sompong; Patramanon,Rina; Uawonggul,Nunthawun; Daduang,Jureerut; Daduang,Sakda.
Abstract  Background: Crude venom of the banded tiger waspVespa affinis contains a variety of enzymes including hyaluronidases, commonly known as spreading factors.  Methods: The cDNA cloning, sequence analysis and structural modelling of V. affinis venom hyaluronidase (VesA2) were herein described. Moreover, heterologous expression and mutagenesis of rVesA2 were performed.  Results: V. affinis venom hyaluronidase full sequence is composed of 331 amino acids, with four predicted N-glycosylation sites. It was classified into the glycoside hydrolase family 56. The homology modelling exhibited a central core (α/&amp;#946;)7 composed of Asp107 and Glu109, acting as the catalytic residues. The recombinant protein was successfully expressed in E. coli with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vespa affinis Hyaluronidase Wasp; Venom Structure analysis Modelling Cloning Protein expression.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100321

Association between the adaptive diagnosis, the indicators of clinical evolution and the object relations test in patients infected by HIV-1, sick or not
Provedor de dados: 80
Autores: Silva Filho,N.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100023

Parametric values of Androctonus crassicauda (Oliver, 1807) (scorpiones: buthidae) from Turkey
Provedor de dados: 80
Autores: Ozkan,O.; Adiguzel,S.; Kar,S.
The aim of the present study was to analyze the parametric values of Androctonus crassicauda (Oliver, 1807), the most significant scorpion species in Turkey. For this purpose, 11 male and 11 female A. crassicauda specimens were collected from Sanliurfa region. The body parts most important for species identification were measured by using sensitive calipers. Differences between sexes were statistically significant for pectinal organ length (female: 7.50±1.26mm;male: 8.63±1.05mm; p=0.033), pectinal tooth count (female: 25.36±0.86;male: 33.36±0.80; p=0.000), and carapace width (female: 10.27±1.19mm;male: 9.09±0.73mm; p=0.000). However, body length (female: 90.09±14.74mm;male: 82.95±5.40mm), abdomen width (female: 12.09±1.90mm;male: 11.09±0.88mm), and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion; Androctonus crassicauda; Morphometry; Turkey.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400003

The first report of Tityus (Scorpiones: Buthidae) in Anzoátegui State, Venezuela: a new species
Provedor de dados: 80
Autores: Quiroga,M.; de Sousa,L.; Parrilla-Álvarez,P.; Manzanilla,J.
Tityus gonzalespongai n. sp. is a species endemic to the high mountains of Anzoátegui State, Venezuela. It is found between 1,600 and 2,200 m in ''La Laguna'' mountain. Its habitat includes the area of ''Bosque Húmedo Montano Bajo'' with the type of vegetation of ''Bosque Ombrófilo Montano Siempreverde'' (''Bosques Nublados Costeros'' that includes the ''Subpáramos Arbustivos''). It is distinguished from other Tityus species (T. caripitensis, T. monaguensis, and T. nororientalis) by the following characteristics: 1- the disposition of the trichobothria in the pedipalps; 2- ventral keels of the caudal segments of the metasoma (segment II, double and parallel in the proximal two thirds, then convergent and finally divergent in the base; segments III and IV,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion; Buthidae; Tityus gonzalespongai; New species; Turimiquire; Anzoátegui; Venezuela.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000100003

Effects of selenium supplementation on cattle anti-rabies humoral immune response and levels of serum selenium and cortisol
Provedor de dados: 80
Autores: Reis,L. S. L. S.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100021

Introducing the CONSORT extension to pilot trials: enhancing the design, conduct and reporting of pilot or feasibility trials
Provedor de dados: 80
Abstract This editorial provides a brief overview of the importance of pilot or feasibility trials or studies, the challenges with current practices in their conduct and reporting, an introduction to the Consolidated Standards of Reporting Trials (CONSORT) extension to pilot trials aimed at improving their reporting, along with some key resources on aspects related to pilot and feasibility studies.
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100101

Fungicidal activity of multinucleated giant cells induced "in vitro" by interferon-gamma and Paracoccidioides brasiliensis antigen
Provedor de dados: 80
Autores: Nascimento,M. P. P. do.
Tipo: Info:eu-repo/semantics/article
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400019

Probable chronic renal failure caused by Lonomia caterpillar envenomation
Provedor de dados: 80
Autores: Schmitberger,Poliana Abrantes; Fernandes,Tássia Clara; Santos,Robson Corrêa; de Assis,Rafael Campos; Gomes,Andréia Patrícia; Siqueira,Priscila Karina; Vitorino,Rodrigo Roger; de Mendonça,Eduardo Gomes; de Almeida Oliveira,Maria Goreti; Siqueira-Batista,Rodrigo.
Erucism is a skin reaction to envenomation from certain poisonous caterpillar bristles. In Brazil, most reports of erucism provoked by Lonomia caterpillars are from the southern region. Most manifestations of erucism are local and include burning pain, itching, local hyperthermia and, rarely, blisters (benign symptoms with spontaneous regression in a few hours). General symptoms such as nausea and vomiting, headache, fever, myalgia, abdominal pain and conjunctivitis may also occur. Uncommon symptoms include arthritis, coagulation disorders (manifested as bruising and bleeding), intracerebral hemorrhage and acute renal failure, which comprise serious complications. The present study reports the case of 60-year-old patient from Rio de Janeiro state, Brazil,...
Tipo: Info:eu-repo/semantics/report
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100501

Ten years of scientific electronic publication: the evolution of The Journal of Venomous Animals and Toxins
Provedor de dados: 80
Autores: Souza,Maria Fernanda Sarmento e.
Tipo: Info:eu-repo/semantics/other
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300001

Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom
Provedor de dados: 80
Autores: Barbosa,AM; Villaverde,AB; Guimarães-Sousa,L; Soares,AM; Zamuner,SF; Cogo,JC; Zamuner,SR.
The prominent myotoxic effects induced by Bothrops jararacussu crude venom are due, in part, to its polycationic myotoxins, BthTX-I and BthTX-II. Both myotoxins have a phospholipase A2 structure: BthTX-II is an active enzyme Asp-49 PLA2, while BthTX-I is a Lys-49 PLA2 devoid of enzymatic activity. In this study, the effect of low-level laser therapy (LLLT), 685 nm laser at a dose of 4.2 J/cm2 on edema formation, leukocyte influx and myonecrosis caused by BthTX-I and BthTX-II, isolated from Bothrops jararacussu snake venom, was analyzed. BthTX-I and BthTX-II caused a significant edema formation, a prominent leukocyte infiltrate composed predominantly by neutrophils and myonecrosis in envenomed gastrocnemius muscle. LLLT significantly reduced the edema...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararacussu; Myotoxins; Inflammation; Myonecrosis; Low-level laser therapy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300014

Comparative analysis of the venom proteome of four important Malaysian snake species
Provedor de dados: 80
Autores: Vejayan,Jaya; Khoon,Too Lay; Ibrahim,Halijah.
BackgroundNaja kaouthia, Ophiophagus hannah, Bungarus fasciatusandCalloselasma rhodostomaare four venomous snakes indigenous to Malaysia. In the present study, their proteomic profile by two-dimensional gel electrophoresis (2-DE) have been separated and compared.ResultsThe 2-DE of venoms of the four species snake demonstrated complexity and obvious interspecies differences in proteome profiles. A total of 63 proteins were identified in the four species: C. rhodostoma -26, N. kaouthia -16, O. hannah -15 and B. fasciatus -6.ConclusionsDespite the identifications of major proteins in the four snake species, a large number of protein spots from the 2-DE were unidentified even though the spots displayed high-quality MALDI-TOF-MS spectra. Those identified...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mass spectrometry; Snake venoms; Proteomics; Two-dimensional gel electrophoresis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200317

A bradykinin-potentiating peptide (BPP-10c) from Bothrops jararaca induces changes in seminiferous tubules
Provedor de dados: 80
Autores: Gilio,Joyce M; Portaro,Fernanda CV; Borella,Maria I; Lameu,Claudiana; Camargo,Antonio CM; Alberto-Silva,Carlos.
Background The testis-specific isoform of angiotensin-converting enzyme (tACE) is exclusively expressed in germ cells during spermatogenesis. Although the exact role of tACE in male fertility is unknown, it clearly plays a critical function in spermatogenesis. The dipeptidase domain of tACE is identical to the C-terminal catalytic domain of somatic ACE (sACE). Bradykinin potentiating peptides (BPPs) from snake venoms are the first natural sACE inhibitors described and their structure-activity relationship studies were the basis for the development of antihypertensive drugs such as captopril. In recent years, it has been showed that a number of BPPs - including BPP-10c - are able to distinguish between the N- and C-active sites of sACE, what is not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bradykinin-potentiating peptide; Snake venom; Angiotensin-converting enzyme; Testis; Seminiferous epithelium.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100316

Haemolymph electrophoretic pattern of Ascia monuste orseis larvae (Lepidoptera: pieridae) parasitized by Cotesia glomerata (Hymenoptera: braconidae)
Provedor de dados: 80
Autores: Scaglia,M.; Brochetto-Braga,M. R.; Chaud-Netto,J.; Gobbi,N.
Cotesia glomerata is a natural enemy of the vegetable plague Ascia monuste orseis and preferably parasites 2nd, 3rd and 4th instar larvae. Parasitism effects on the haemolymph protein profile of Ascia monuste orseis larvae from the 2nd to 7th days were analyzed both qualitatively and quantitatively by SDS-PAGE and Coomassie-Blue binding methods. Quantitative analysis showed a progressive increase in the protein content of about 6.5 and 12.5 times in parasitized and non-parasitized larvae from the 2nd to 5th days, respectively. On the 6th day, a decrease in protein content was observed in both groups, although this decrease was significantly less than the control group that continued to metamorphosis. Meanwhile, parasitized larvae had one more day (7th day)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascia monuste orseis; Cotesia glomerata; Electrophoretic protein profile; Haemolymph; Natural enemy; Parasitism; SDS-PAGE.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100006

Biochemical and pharmacological study of venom of the wolf spider Lycosa singoriensis
Provedor de dados: 80
Autores: Liu,ZH; Qian,W; Li,J; Zhang,Y; Liang,S.
The wolf spider Lycosa singoriensis is a large and venomous spider distributed throughout northwestern China. Like other spider venoms, the wolf spider venom is a chemical cocktail. Its protein content is 0.659 mg protein/mg crude venom as determined by the Lowry method. MALDI-TOF analysis revealed that the venom peptides are highly diverse and may be divided into three groups characterized by three independent molecular ranges: 2,000 to 2,500 Da, 4,800 to 5,500 Da and 7,000 to 8,000 Da, respectively. This molecular distribution differs substantially from those of most spider venoms studied so far. This wolf spider venom has low neurotoxic action on mice, but it can induce hemolysis of human erythrocytes. Furthermore, the venom shows antimicrobial activity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spider; Lycosa singoriensis; Crude venom; MALDI-TOF; Antimicrobial activity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100008

Paths to the discovery of antivenom serotherapy in France
Provedor de dados: 80
Autores: Bochner,Rosany.
Abstract The current study presents a descriptive chronological survey of the articles published by Césaire Auguste Phisalix and Albert Calmette on snake poison, with the aim of shedding a light on the areas of research and reasoning followed by these scientists, leading up to their simultaneous discovery of antivenom serotherapy in 1894. The path taken by Phisalix is revealed in 15 articles that demonstrate the motivation of a naturalist and the way he confronted the puzzle of immunity against snake venom. In the case of Calmette, two articles preceded the discovery; microbiology was his theoretical base and the Pasteurian spirit of solving health problems his driving force. These two researchers followed distinct paths, mobilized by different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calmette; Phisalix; Antivenom serotherapy; Snake; History of science.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100201

Antibiotic therapy for snakebite envenoming
Provedor de dados: 80
Autores: Resiere,Dabor; Gutiérrez,José María; Névière,Rémi; Cabié,André; Hossein,Mehdaoui; Kallel,Hatem.
Abstract There are numerous conflicting recommendations available on the use of antibiotics following snakebite. The present letter to the editor presents some recommendations based on recent studies, and aims to stimulate debate on this topic.
Tipo: Info:eu-repo/semantics/other
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100701

Epidemiological profile of snakebites in Morocco
Provedor de dados: 80
Autores: Arfaoui,A; Hmimou,R; Ouammi,L; Soulaymani,A; Mokhtari,A; Chafiq,F; Soulaymani-Bencheikh,R.
The present work aims to find the epidemiological profile of snakebites in Morocco through a retrospective study of 1,423 snakebite cases that occurred between 1992 and 2007. Data were obtained from medical charts of envenomation at the Poison Control and Pharmacovigilance Center of Morocco. Results revealed that 86% of the snakebites had occurred in rural areas and that males were significantly more affected than females at a sex ratio of 23:20. Furthermore, 35% of the bites happened during the summer, with a peak of 215 cases in June (15.1%). We also discovered that 67.3% of the patients were bitten during the day. The age group that comprised the most agriculturally active persons, from 20 to 44 years old, was the most affected by snakebites (551...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snakebites; Epidemiology; Mortality; Morocco.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400005

Clinical and epidemiological characteristics of injuries caused by venomous snakes observed at the hospital for tropical diseases of Araguaína, Tocantins State, Brazil, from 1995 to 2000
Provedor de dados: 80
Autores: Paula Neto,J. B.; Ribeiro,R. S. P.; Luz,J. A.; Galvão,M.; Carvalho,S. M. D.; Haddad Junior,V.
Injuries caused by venomous snakes are considered a problem of public health in Brazil, and further studies for a better knowledge are very important. The aim of this work is to describe clinical and epidemiological characteristics that were observed from 1995 to 2000 in the Hospital for Tropical Diseases of Araguaína, Tocantins State, Brazil, which is a unit of health that takes care of patients suffering from tropical diseases. We studied 440 individuals and the most common characteristics observed were masculine sex, age among 15-45 years, bite in low members, and injuries caused by snakes of the Bothrops genus. The signs and symptoms more frequently observed were edema, pain, erythema, and bleeding. The most observed systemic manifestation was...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Snakebite; Tocantins State; Brazil; Epidemiology.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400004

Catostylus tagi: partial rDNA sequencing and characterisation of nematocyte structures using two improvements in jellyfish sample preparation
Provedor de dados: 80
Autores: Parracho,Tiago; Morais,Zilda.
AbstractBackground More than 200 Scyphozoa species have been described, but few have been properly studied regarding their chemical and genetic characteristics.Catostylus tagi, an edible Scyphozoa and the sole European Catostylidae, occurs in summer at Tagus and Sado estuaries. Neither a systematic comparison between the two Catostyluscommunities nor a chemical approach on their nematocytes had been carried out yet.Methods In order to achieve these purposes, optimisation of DNA extraction and of histochemical staining procedures were developed.Catostylus specimens from Tagus and Sado estuaries were compared by ribosomal 18S, 28S, and ITS1 partial sequencing. The morphochemistry of nematocytes was studied by optical and electronic microscopy.Results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genomic DNA extraction; 18S 28S ITS1 ribosomal DNA; Structural macromolecules of nematocytes; Catostylus tagi jellyfish.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100346

American visceral Leishmaniasis: a case report
Provedor de dados: 80
Autores: Langoni,H.; Lucheis,S. B.; Da Silva,R. C.; Castro,A. P. B.; Paes,A. C.
Visceral leishmaniasis is a zoonotic disease caused by parasites of the Leishmania genus. Dog is the major source of infection to man, especially in urban areas. The authors report a case of visceral leishmaniasis in a pit bull female dog from Bocaina, São Paulo, Brazil. The animal presented clinical signs compatible with leishmaniasis, including skin lesions in the body and partial damage of the external ears. The indirect fluorescent antibody test (IFAT) demonstrated a titer of 1280, and promastigote forms of Leishmania sp were isolated by the culture of bone marrow puncture. Cytological analysis of the lymph node and smear of the bone marrow puncture revealed macrophages containing amastigote forms of Leishmania sp in their inner region. The test of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Zoonosis; Dog; Diagnosis; Hemoculture; Serology; Polymerase chain reaction (PCR).
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300012

Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review
Provedor de dados: 80
Autores: Paiva-Cavalcanti,M; Regis-da-Silva,CG; Gomes,YM.
In recent years, the polymerase chain reaction (PCR) technique has significantly advanced towards expanding its use and versatility by working with quantitative real-time PCR (qPCR). Data from the literature show that both methods present interesting characteristics for the diagnosis of visceral leishmaniasis. The benefits of qPCR in relation to conventional PCR include speed, reproducibility and quantitative ability. In addition to operational advantages, qPCR is more sensitive and reproducible and may replace conventional PCR in diagnostic routines. Regarding visceral leishmaniasis, the possibility of deployment of real-time PCR in highly complex diagnoses (reference services) in endemic areas will facilitate a swift and safe return for patients....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Molecular diagnosis; Visceral leishmaniasis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400004

Cytotoxic and pro-apoptotic action of MjTX-I, a phospholipase A2 isolated from Bothrops moojeni snake venom, towards leukemic cells
Provedor de dados: 80
Autores: Benati,Rogério Bodini; Costa,Tássia Rafaela; Cacemiro,Maira da Costa; Sampaio,Suely Vilela; Castro,Fabíola Attié de; Burin,Sandra Mara.
Abstract  Background: Chronic myeloid leukemia (CML) is a BCR-ABL1+ myeloproliferative neoplasm marked by increased myeloproliferation and presence of leukemic cells resistant to apoptosis. The current first-line therapy for CML is administration of the tyrosine kinase inhibitors imatinib mesylate, dasatinib or nilotinib. Although effective to treat CML, some patients have become resistant to this therapy, leading to disease progression and death. Thus, the discovery of new compounds to improve CML therapy is still challenging. Here we addressed whether MjTX-I, a phospholipase A2 isolated from Bothrops moojeni snake venom, affects the viability of imatinib mesylate-resistant Bcr-Abl+ cell lines.  Methods: We examined the cytotoxic and pro-apoptotic effect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic myeloid leukemia; Bcr-Abl; Phospholipase A2; MjTX-I; Bothrops moojeni; Apoptosis; Cytotoxicity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100330

Nitro-Heterocyclic compounds induce apoptosis-like effects in Leishmania (L). amazonensis promastigotes
Provedor de dados: 80
Autores: Mendonça,Daiane Barros Dias; Silva,Renata Ellen Costa; Palace-Berl,Fanny; Takakura,Cleusa FH; Soares,Sandra Regina C; Braz,Lucia Maria Almeida; Tavares,Leoberto Costa; Lindoso,Jose Angelo Lauletta.
Abstract  Background: Three drugs - pentavalent antimonials, amphotericin B and pentamidine - are currently used for leishmaniasis treatment. They are administered for long periods, only parenterally, and have high cardiac, renal and hepatic toxicities. Therefore, the investigation of new compounds is required. Nitro-heterocyclic derivatives have been used as possible drug candidates to treat diseases caused by trypanosomatids.  Methods: Leishmania (L.) amazonensis promastigotes (MHO/BR/73/M2269), maintained in the Laboratório de Soroepidemiologia - Instituto de Medicina Tropical- USP, were exposed to five nitroheterocyclic derivatives, with differences at phenyl-ring position 4: BSF-C4H9, BSF-H, BSF-NO2, BSF-CH3 and BSF-Cl, for 48 hours. After analyzing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis treatment; Nitro-heterocyclic compounds; Leishmania (L.) amazonensis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100303

Tityus serrulatus envenoming in non-obese diabetic mice: a risk factor for severity
Provedor de dados: 80
Autores: Oliveira,Guilherme Honda de; Cerni,Felipe Augusto; Cardoso,Iara Aimê; Arantes,Eliane Candiani; Pucca,Manuela Berto.
Abstract  Background  In Brazil, accidents with venomous animals are considered a public health problem. Tityus serrulatus (Ts), popularly known as the yellow scorpion, is most frequently responsible for the severe accidents in the country. Ts envenoming can cause several signs and symptoms classified according to their clinical manifestations as mild, moderate or severe. Furthermore, the victims usually present biochemical alterations, including hyperglycemia. Nevertheless, Ts envenoming and its induced hyperglycemia were never studied or documented in a patient with diabetes mellitus (DM). Therefore, this is the first study to evaluate the glycemia during Ts envenoming using a diabetic animal model (NOD, non-obese diabetic).  Methods  Female mice (BALB/c...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tityus serrulatus; Diabetes mellitus; Scorpion venom; NOD mice; Glycemia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100319

Echocardiographic changes during acute pulmonary edema subsequent to scorpion sting
Provedor de dados: 80
Autores: Delma,K.
Acute pulmonary edema (APE) occurring after scorpion sting is the leading cause of death of the victims of scorpion envenomation. The APE origin is still questioned by physicians treating these patients. Based on echocardiographic study of 20 patients with severe envenomation treated in Ouargla Hospital resuscitation ward during the last four years, the APE etiology seems more likely cardiogenic, referring to cardiac symptoms confirmed by echocardiography although other mechanisms may also be involved. This hypothesis is further confirmed by the positive response of patients to the administration of dobutamine.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion envenomation; Acute pulmonary edema; Echocardiography; Dobutamine.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400010

Ultramorphological analysis of the venom glands and their histochemical relationship with the convoluted glands in the primitive social paper wasp Polistes versicolor (Hymenoptera: Vespidae)
Provedor de dados: 80
Autores: Britto,F. B.; Caetano,F. H.
The venom glands are part of the most important defense weapon in Aculeata: the venom apparatus. The arrangement of these glands can vary among species, but in general they are composed of long secretory tubules connected to a muscular sac-like reservoir. Although the occurrence of these variations has been documented, many studies neglected the existence of a well-developed secretory portion in the lumen of the reservoir named convoluted gland. This study is an ultramorphological analysis of the venom glands and their histochemical relationship with the convoluted glands in the primitive social wasp Polistes versicolor. In this wasp, the venom glands are constituted by two tubular portions that penetrate individually in the venom reservoir, inside of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wasps; Venom glands; Convoluted glands; Ultramorphology; Histochemistry.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200007

Propolis: a review of its anti-inflammatory and healing actions
Provedor de dados: 80
Autores: Ramos,A. F. N.; Miranda,J. L.
Tissue healing is an adaptive biological response by which the organism repairs damaged tissue. The initial stage of healing is represented by an acute inflammatory reaction, in which inflammatory cells migrate to damaged tissue and phagocyte debris. At a later stage, fibroblasts and endothelial cells proliferate and generate a scar. The occurrence of inflammatory processes and healing imperfections have been a concern for hundreds of years, especially for individuals with healing difficulties, such as diabetics and carriers of peripheral circulation deficiencies. A wide variety of natural products have been used as anti-inflammatory and healing agents, with propolis being a remarkable option. Propolis has been used in popular medicine for a very long...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propolis; Inflammation; Anti-inflammatory action; Healing properties; Apis mellifera.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400002

Partial purification and functional characterization of Ts19 Frag-I, a novel toxin from Tityus serrulatus scorpion venom
Provedor de dados: 80
Autores: Lima,Priscila C.; Bordon,Karla C. F.; Pucca,Manuela B.; Cerni,Felipe A.; Zoccal,Karina F.; Faccioli,Lucia H.; Arantes,Eliane C.
Abstract  Background  The yellow scorpion Tityus serrulatus (Ts) is responsible for the highest number of accidents and the most severe scorpion envenoming in Brazil. Although its venom has been studied since the 1950s, it presents a number of orphan peptides that have not been studied so far. The objective of our research was to isolate and identify the components present in the fractions VIIIA and VIIIB of Ts venom, in order to search for a novel toxin. The major isolated toxins were further investigated for macrophage modulation.  Methods  The fractions VIIIA and VIIIB, obtained from Ts venom cation exchange chromatography, were rechromatographed on a C18 column (4.6 × 250 mm) followed by a reversed-phase chromatography using another C18 column (2.1 ×...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-KTx; Cytokine; Interleukin; Neurotoxin; Pro-inflammatory; Scorpion venom; Tityus serrulatus.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100353

Susceptibility profile of Salmonella against the antibacterial activity of propolis produced in two regions of Brazil
Provedor de dados: 80
Autores: Orsi,R. O.; Sforcin,J. M.; Rall,V. L. M.; Funari,S. R. C.; Barbosa,Luciano; Fernandes,JR A.
Propolis antibiotic action has been widely investigated. This assay was carried out in order to observe the in vitro antibacterial activity of propolis against Salmonella enteritidis isolated from food and Salmonella typhimurium isolated from human infections. Propolis was collected by Apis mellifera in two regions of Brazil (Mossoró, Rio Grande do Norte State; and Urubici, Santa Catarina State). Both strains survival percentage decreased with time of incubation in Ethanolic Extracts of Propolis (EEP), demonstrating bactericidal effect after 24 hours. It was also observed that EEP from Mossoró was more effective than that from Urubici. The control of the propolis solvent - 70% ethanol - was less effective than EEP, showing only a bacteriostatic effect. We...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propolis; Susceptibility profile; Salmonella; Food; Infection.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200003

Association between handling stress in the corral and rabies antibody titers in selenium-supplemented cattle
Provedor de dados: 80
Autores: Reis,LSLS; Chiacchio,SB; Oba,E; Pardo,PE; Frazatti-Gallina,NM.
This study determined the correlation between serum cortisol levels and rabies antibody titers in cattle primo-vaccinated against rabies and supplemented with dietary selenium (Se). Sixty Nelore male calves (10 to 12 months old) received daily and individual dietary supplementation with 0, 3.6, 5.4 and 6.4 mg Se (groups Gc, G3.6, G5.4 and G6.4, respectively). The animals were vaccinated against rabies (day 0) and subjected to handling stress in the corral for 120 days. Blood sampling procedures were performed on days 0, 15, 30, 60, 90 and 120. Cortisol levels increased until day 90, but had dropped significantly by day 120 (P &lt; 0.01). Rabies antibody titers on days 30 and 90 were similar among Se-supplemented groups; in the control group, rabies...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cattle; Cortisol; Stress; Rabies immune response; Selenium supplementation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400015

Prostatic cryptococcosis: a case report
Provedor de dados: 80
Autores: Chang,M. R.; Paniago,A. M. M.; Silva,M. M.; Lazéra,M. S.; Wanke,B.
Cryptococcosis is a systemic mycosis usually affecting immunodeficient individuals. In contrast, immunologically competent patients are rarely affected. Dissemination of cryptococcosis usually involves the central nervous system, manifesting as meningitis or meningoencephalitis. Prostatic lesions are not commonly found. A case of prostate cryptococcal infection is presented and cases of prostatic cryptococcosis in normal and immunocompromised hosts are reviewed. A fifty-year-old HIV-negative man with urinary retention and renal insufficiency underwent prostatectomy due to massive enlargement of the organ. Prostate histopathologic examination revealed encapsulated yeast-like structures. After 30 days, the patient's clinical manifestations worsened, with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryptococcosis; Cryptococcus; Immunocompetent patient; Cryptococcal prostatitis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200015

Envenomation by the madagascan colubrid snake, Ithycyphus miniatus
Provedor de dados: 80
Autores: Mori,A.; Mizuta,T.
We report two cases of envenomation by a Madagascan opisthoglyphous snake, Ithycyphus miniatus. In both cases, the snake bit the finger of a human who was preparing an experiment by tying a string around the snake body. Symptoms of the first case included temporal severe local pain and extensive bleeding. In the second case, severe pain accompanying obvious local swelling was caused and lasted for several hours. The present observations indicate that bite by I. miniatus potentially causes serious physiological effects in humans although the snake is basically calm and reluctant to bite.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Madagascar; Colubrid; Ithycyphus miniatus; Envenomation; Opisthoglyph; Duvernoy's glands.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300013

What is the most dangerous snake?
Provedor de dados: 80
Autores: McCue,Marshall D.
Letter to Editor of Journal of Venomous Animals and Toxins.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Snakes; Snake bites; Snake venoms; Snake envenomation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100101

Bordonein-L, a new L-amino acid oxidase from Crotalus durissus terrificus snake venom: isolation, preliminary characterization and enzyme stability
Provedor de dados: 80
Autores: Bordon,Karla C. F.; Wiezel,Gisele A.; Cabral,Hamilton; Arantes,Eliane C.
BackgroundCrotalus durissus terrificus venom (CdtV) is one of the most studied snake venoms in Brazil. Despite presenting several well known proteins, its L-amino acid oxidase (LAAO) has not been studied previously. This study aimed to isolate, characterize and evaluate the enzyme stability of bordonein-L, an LAAO from CdtV.Methods The enzyme was isolated through cation exchange, gel filtration and affinity chromatography, followed by a reversed-phase fast protein liquid chromatography to confirm its purity. Subsequently, its N-terminal amino acid sequence was determined by Edman degradation. The enzyme activity and stability were evaluated by a microplate colorimetric assay and the molecular mass was estimated by SDS-PAGE using periodic acid-Schiff...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durissus terrificus; L-amino acid oxidase; Rattlesnake; Enzyme activity; Enzyme stability; Chromatography; Snake venom; Yellow venom; Stabilization.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100335

Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms
Provedor de dados: 80
Autores: Ferreira Junior,R. S.; Nascimento,N.; Martinez,J. C.; Alves,J. B.; Meira,D. A.; Barraviera,B.
ELISA was used to evaluate, accompany, and compare the humoral immune response of Swiss mice during hyperimmunization with native and Cobalt-60-irradiated (60Co) venoms of Bothrops jararaca, Bothrops jararacussu and Bothrops moojeni. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by in vivo challenge, and the side effects were assessed. The animals immunization with one LD50 of each venom occurred on days 1, 15, 21, 30, and 45, when blood samples were collected; challenges happened on the 60th day. Results showed that ELISA was efficient in evaluating, accompanying and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venom; Irradiation; Bothrops; ELISA; Serum production; Detoxification.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400006

Retrospective analysis of post-exposure to human anti-rabies treatment in Botucatu, São Paulo State, Brazil
Provedor de dados: 80
Autores: Ayres,JA; Paiva,BSR; Barraviera,B.
This analysis aimed to identify characteristics of accidents that would, probably, provoke rabies infection. A total of 14,409 survey questionnaires for surveillance of human rabies from the Brazilian Information System for Disease Notification (SINAN), from 2000 to 2005, were analyzed. Regarding demographics, it was observed that 7,377 (51.5%) of the victims were white, 4,458 (30.93%) were children and 8,008 (55.58%) were males. Urban cases were prevalent (88.10%) while dogs were the animals most frequently involved in accidents, in 11,700 cases (81.19%). Bites (84.35%) and scratches (19.15%) were the most prevalent exposure types, and occurred predominantly on victims' extremities (38.79%). The prophylactic measure taken in 6,179 cases comprised...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Post-exposure prophylaxis; Anti-rabies serotherapy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100016

Neutralization of the neuromuscular activity of bothropstoxin-i, a myotoxin from Bothrops jararacussu snake venom, by a hydroalcoholic extract of Casearia sylvestris Sw. (guaçatonga)
Provedor de dados: 80
Autores: Oshima-Franco,Y.; Alves,C. M. V.; Andréo Filho,N.; Gerenutti,M.; Cintra,A. C. O.; Leite,G. B.; Rodrigues-Simioni,L.; Silva,M. G.
Numerous plants are used as snakebite antidotes in Brazilian folk medicine, including Casearia sylvestris Swartz, popularly known as guaçatonga. In this study, we examined the action of a hydroalcoholic extract from C. sylvestris on the neuromuscular blockade caused by bothropstoxin-I (BthTX-I), a myotoxin from Bothrops jararacussu venom, in mouse isolated phrenic nerve-diaphragm (PND) preparations. Aqueous (8 and 12 mg/ml, n=4 and 5, respectively) and hydroalcoholic (12 mg/ml, n=12) extracts of the leaves of C. sylvestris caused facilitation in PND preparations followed by partial neuromuscular blockade. BthTX-I (20 µg/ml, n=4) caused 50% paralysis after 65±15 min (mean ± S.E.M). Preincubation (30 min at 37° C) of BthTX-I (20 µg/ml, n=4) with a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alternative medicine; Phytotherapy; Plant extracts; Snakebite.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400007

Indice latinoamericano de publicaciones científicas seriadas
Provedor de dados: 80
Autores: Sarmento e Souza,M. F.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100001

Second-generation pterocarpanquinones: synthesis and antileishmanial activity
Provedor de dados: 80
Autores: Faiões,Viviane dos Santos; Frota,Lívia C. R. M. da; Cunha-Junior,Edézio Ferreira; Barcellos,Julio C. F.; Silva,Thayssa Da; Daher Netto,Chaquip; Da-Silva,Silvia Amaral Gonçalves; Silva,Alcides J. M. da; Costa,Paulo R. R.; Torres-Santos,Eduardo Caio.
Abstract  Background: Despite the development of new therapies for leishmaniasis, among the 200 countries or territories reporting to the WHO, 87 were identified as endemic for Tegumentary Leishmaniasis and 75 as endemic for Visceral Leishmaniasis. The identification of antileishmanial drug candidates is essential to fill the drug discovery pipeline for leishmaniasis. In the hit molecule LQB-118 selected, the first generation of pterocarpanquinones was effective and safe against experimental visceral and cutaneous leishmaniasis via oral delivery. In this paper, we report the synthesis and antileishmanial activity of the second generation of pterocarpanoquinones.  Methods: The second generation of pterocarpanquinones 2a-f was prepared through a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Pterocarpanquinone; LQ-118; Phenotypic assay; Leishmaniasis; Drug discovery; Neglected diseases.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100325

Vision loss following snakebite in a patient with controlled aplastic anemia
Provedor de dados: 80
Autores: Kweon,EY; Lee,DW; Ahn,M; Nork,TM; Cho,NC.
Viper venoms act mainly on blood and blood vessels. Reports of ophthalmic manifestations after snakebite include ptosis and ophthalmoplegia. In the current study, we describe a case that developed bilateral retinal and subretinal hemorrhage following snakebite. Bilateral retinal hemorrhage is a rare ocular complication of snake envenomation and has not been reported with fundus photographs in the literature so far.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snakebite; Retinal hemorrhage; Visual loss; Aplastic anemia.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100015

A new scenario of bioprospecting of Hymenoptera venoms through proteomic approach
Provedor de dados: 80
Autores: Santos,LD; Pieroni,M; Menegasso,ARS; Pinto,JRAS; Palma,MS.
Venoms represent a huge and essentially unexplored reservoir of bioactive components that may cure diseases that do not respond to currently available therapies. This review select advances reported in the literature from 2000 to the present about the new scenario of Hymenoptera venom composition. On account of new technologies in the proteomic approach, which presents high resolution and sensitivity, the combination of developments in new instruments, fragmentation methods, strategic analysis, and mass spectrometry have become indispensable tools for interrogation of protein expression, molecule interaction, and post- translational modifications. Thus, the biochemical characterization of Hymenoptera venom has become a major subject of research in the area...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hymenoptera; Venoms; Allergens; Phospholipase A1; Wasp venom antigen 5; Hyaluronoglucosaminidase; Mass spectrometry; Proteomics.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400003

Multiple uses of fibrin sealant for nervous system treatment following injury and disease
Provedor de dados: 80
Autores: Biscola,Natalia Perussi; Cartarozzi,Luciana Politti; Ulian-Benitez,Suzana; Barbizan,Roberta; Castro,Mateus Vidigal; Spejo,Aline Barroso; Ferreira Jr.,Rui Seabra; Barraviera,Benedito; Oliveira,Alexandre Leite Rodrigues.
Abstract Lesions to the nervous system often produce hemorrhage and tissue loss that are difficult, if not impossible, to repair. Therefore, scar formation, inflammation and cavitation take place, expanding the lesion epicenter. This significantly worsens the patient conditions and impairment, increasing neuronal loss and glial reaction, which in turn further decreases the chances of a positive outcome. The possibility of using hemostatic substances that also function as a scaffold, such as the fibrin sealant, reduces surgical time and improve postoperative recovery. To date, several studies have demonstrated that human blood derived fibrin sealant produces positive effects in different interventions, becoming an efficient alternative to suturing. To...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Central nervous system; Peripheral nervous system; Commercial fibrin sealant; New heterologous fibrin sealant; Nervous system injury; Fibrin tissue adhesive.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100202

Nutritional state of HCV-infected individuals examined and treated in outpatient clinic
Provedor de dados: 80
Autores: Carreira,C. M.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100016

Building the knowledge society by opening access to scholarly information
Provedor de dados: 80
Autores: Kuramoto,Hélio.
Tipo: Info:eu-repo/semantics/other
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400001

Bioclimatic distribution and prevalence maps forFasciola hepatica in Espírito Santo State, Brazil
Provedor de dados: 80
Autores: Freitas,Deivid França; Martins,Isabella VF; Santos,Gleissy MADA dos; Santos,Alexandre R dos; Gomes,Daniel da Silva.
BackgroundFasciolosis affects different ruminant species and leads to great economic losses for cattle farmers worldwide. Thus, the current study aimed to evaluate bovine fasciolosis prevalence in the state of Espírito Santo, Brazil, using slaughter maps provided by slaughterhouses and verifying the origin of cattle.Methods : A map was created based on analysis of epidemiological data. The ArcGIS/ArcINFO 10.1 software was employed in order to elaborate updated bioclimatic maps that displayed the fasciolosis prevalence within the state – per city– between 2009 and 2011.ResultsAccording to the bioclimatic map it was clear that 52.24% of the state’s total area comprise regions considered favorable for the development and survival of Fasciola hepatica....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maps; Geographic information system (GIS); Prevalence; Fasciola hepatica.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200333

Laryngeal lesions and resulting dysphonia in patients with paracoccidioidomycosis
Provedor de dados: 80
Autores: Weber,S. A. T.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100024

Treatment of venous ulcers with fibrin sealant derived from snake venom
Provedor de dados: 80
Autores: Gatti,MAN; Vieira,LM; Barraviera,B; Barraviera,SRCS.
Venous ulcers of the lower limbs complicated by infection or chronicity represent a serious public health problem. The elevated number of those afflicted burdens the health services, interferes in quality of life and causes absenteeism. Although there are 2,500 items on the market, ranging from the simplest dressing up to the most complex types of dressing, treatment remains a challenge. Among the substances used, fibrin sealant is the one that promotes diminution of bacterial colonization and of edema, controls hemorrhaging, alters the pain threshold by protecting the nerve endings, hydrates the wound bed and forms granulation tissue that favors healing. Its disadvantages include higher cost and utilization of human fibrinogen that can transmit infectious...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Fibrin sealant; Healing; Wounds; Venous ulcers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200015

Perinatal effects of scorpion venoms: maternal and offspring development
Provedor de dados: 80
Autores: Dorce,Ana Leticia Coronado; Martins,Adriana do Nascimento; Dorce,Valquiria Abrão Coronado; Nencioni,Ana Leonor Abrahão.
Abstract Scorpion envenomation is a public health problem, especially in tropical and subtropical countries. Considering the high incidence of scorpionism in some areas, pregnant women and nursing mothers may be possible victims. Scorpion stings alter the release of neurotransmitters and some cytokines. These mediators act as organizers and programmers in the adequate formation of the nerves, and non-physiological concentrations of them during the brain organization originate disorders and diseases that can appear later in the life of the individual. Despite the importance of this subject, there are only a few studies showing the effects of scorpion venom on maternal reproductive development, in the morphology and physical and behavioral development of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venoms; Pregnancy; Lactation; Reproductive development; Perinatal development.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100210

Effects of atropine and propranolol on lung inflammation in experimental envenomation: comparison of two buthidae venoms
Provedor de dados: 80
Autores: Saidi,Hadjer; Adi-Bessalem,Sonia; Hammoudi-Triki,Djelila; Laraba-Djebari,Fatima.
Background Previous works had shown that scorpion venom induced neurotransmitter elevation and an inflammatory response associated with various anatomo-pathological modifications. The most dangerous scorpions species in Algeria responsible for these effects are Androctonus australis hector (Aah) and Androctonus amoreuxi (Aam). Results Comparison of the physiopathological effects induced by the two venoms showed differences in the kinetic of cytokine release and in lung injury. The lung edema was only observed in response to Aah venom and it was correlated with cell infiltration. In order to better understand the involved mechanism in inflammatory response, we used two antagonists, atropine (non-selective muscarinic antagonist) and propranolol (β adrenergic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venoms; Cytokines; Lung inflammation; Acetylcholine; Atropine; Propranolol.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100305

In vivo effects of Buthus occitanus tunetanus and Androctonus australis garzoni scorpion venoms on pregnant and non-pregnant rats
Provedor de dados: 80
Autores: Hmed,B. N.; Serria,H. T.; Selma,C.; Zouheir,S.; Tarek,R.; Mondher,K.; Zakaria,B.; Mounir,Z. K.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venom; Buthus occitanus tunetanus; Androctonus australis garzoni; In vivo effects; Gravid rats.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200013

Antinociceptive effect of PnTx4(5-5), a peptide from Phoneutria nigriventer spider venom, in rat models and the involvement of glutamatergic system
Provedor de dados: 80
Autores: Oliveira,Camila Franco Batista; Alves,Daniela Pereira; Emerich,Bruna Luiza; Figueiredo,Suely Gomes de; Cordeiro,Marta do Nascimento; Borges,Márcia Helena; Richardson,Michael; Pimenta,Adriano Monteiro de Castro; Duarte,Igor Dimitri Gama; Lima,Maria Elena de.
ABSTRACT  Background:  The venom of Phoneutria nigriventer spider is a source of numerous bioactive substances, including some toxins active in insects. An example is PnTx4(5-5) that shows a high insecticidal activity and no apparent toxicity to mice, although it inhibited NMDA-evoked currents in rat hippocampal neurons. In this work the analgesic activity of PnTx4(5-5) (renamed Γ-ctenitoxin-Pn1a) was investigated.  Methods:  The antinociceptive activity was evaluated using the paw pressure test in rats, after hyperalgesia induction with intraplantar injection of carrageenan or prostaglandin E2 (PGE2).  Results:  PnTx4(5-5), subcutaneously injected, was able to reduce the hyperalgesia induced by PGE2 in rat paw, demonstrating a systemic effect. PnTx4(5-5)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spider toxin; Γ-ctenitoxin-Pn1a; PnTx4(5-5); Phoneutria nigriventer; Antinociception; Glutamate.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100316

On the interference of clinical outcome on rabies transmission an perpetuation
Provedor de dados: 80
Autores: Brandão,PE.
Rabies is a viral zoonotic infectious disease that affects mammals and is caused by genotypes/species of the Lyssavirus genus (Rhabdoviridae, Mononegavirales), with the genotype 1 (classic rabies virus - RABV) being the most prevalent. Despite continuous efforts, rabies is still an incurable disease that causes thousands of deaths amongst humans worldwide. Due to a wide range of hosts and the different evolutionary paths of RABV in each host, several host-specific variants have arisen in an ongoing process. The result of RABV replication in nervous tissues may lead to two opposite clinical outcomes, i.e., paralytic/dumb form and encephalitic/furious one. The paralytic form creates dead-end hosts mainly amongst herbivores, while the furious form of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Transmission; Disease ecology; Epidemiology; Immune response.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200003

Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART)
Provedor de dados: 80
Autores: Meira,DA; Almeida,RAMB; Barbosa,AN; de Souza,LR; Olivo,TET; Henriques,RMS; Golim,MA; Araújo Jr,JP; Nagoshi,LR; Orikaza,CM; Calvi,SA.
A cross-sectional study was performed on HIV-1 infected individuals with or without antiretroviral treatment (ARV) in the AIDS Day Hospital, Botucatu Medical School, UNESP. Between August 2004 and October 2005, 73 HIV-1 infected individuals were divided into three groups: infected individuals with or without AIDS who had never received ARV (G1 = 15); patients on HAART that had had plasma HIV-1 RNA viral load (VL) equal to or greater than 50 copies/mL (G2 = 27); and patients on HAART with undetectable VL for at least the past six months (G3 = 31). There was also an additional group that comprised blood donors without any sign of the disease and with negative HIV serum tests (G4 = 20), which was the control group. Serum cytokine levels (values in pg/mL) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RT-PCR; ELISA; Cytokines; HIV; AIDS; HAART; Apoptosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400011

Biochemical and morphological analysis of cell death induced by Egyptian cobra (Naja haje) venom on cultured cells
Provedor de dados: 80
Autores: Omran,M. A. A.; Fabb,S. A.; Dickson,G.
We investigated the in vitro process of cell death caused by Egyptian cobra venom on primary human embryonic kidney (293T) and mouse myoblast (C2C12) cell lines. The aim of these studies was to provide further information about triggering cell death, and suggest methods for eliminating unwanted cells, such as tumour cells. Both cell lines were treated with 10, 20, and 50 m g/ml of Egyptian cobra (Naja haje) venom in serum free media (SFM) and incubated for 8 hours. Total activities of the lactate dehydrogenase (LDH) and creatine kinase (CK) released in the culture during venom incubation were used as an indicator of the venom in vitro cytotoxicity. Cell injury was morphologically recognized and apoptosis determined by a Fluorescing Apoptosis Detection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cobra venom; Cytotoxicity; Apoptosis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300004

Snakebite envenomation turns again into a neglected tropical disease!
Provedor de dados: 80
Autores: Chippaux,Jean-Philippe.
Abstract: On June 9th, 2017 WHO categorized snakebite envenomation into the Category A of the Neglected Tropical Diseases. This new situation will allow access to new funding, paving the way for wider and deeper researches. It should also expand the accessibility of antivenoms. Let us hope that it also leads to cooperation among stakeholders, aiming at improving the management of snakebites in developing countries.
Tipo: Info:eu-repo/semantics/other
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100802

Bufotenine is able to block rabies virus infection in BHK-21 cells
Provedor de dados: 80
Autores: Vigerelli,Hugo; Sciani,Juliana Mozer; Jared,Carlos; Antoniazzi,Marta Maria; Caporale,Graciane Maria Medeiros; Silva,Andréa de Cássia Rodrigues da; Pimenta,Daniel C.
Background Rabies is a fatal zoonotic neglected disease that occurs in more than 150 countries, and kills more than 55.000 people every year. It is caused by an enveloped single stranded RNA virus that affects the central nervous system, through an infection initiated by the muscular nicotinic acetylcholine receptor, according to many authors. Alkaloids, such as acetylcholine, are widespread molecules in nature. They are present in numerous biological fluids, including the skin secretion of many amphibians, in which they act (together with proteins, peptides and steroids) as protection agents against predators and/or microorganisms. Among those amphibians that are rich in alkaloids, there is the genus Rhinella.Methods Bufotenine was isolated from Rhinela...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Rhinella; Bufotenine; Alkaloids; Toxins; Mass spectrometry.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200336

Experimental murine chromoblastomycosis obtained from Fonsecaea pedrosoi isolate cultured for a long periodt
Provedor de dados: 80
Autores: Machado,AP; Freymuller,E; Fischman,O.
The present study aimed to describe F. pedrosoi propagules capable of causing chronic murine disease. Several changes in F. pedrosoi hyphae were identified in fungal cells cultured for a long period. Optical microscopy found many rounded cells with double-rigid melanin-rich walls. Terminal and intercalary chlamydoconidia were also frequently observed. Analyses of images from transmission electron microscopy (TEM) revealed several cells with walls composed of at least three layers and an outer layer enriched with melanin. Two groups of twenty BALB/c mice were subcutaneously infected in their footpads with F. pedrosoi cells at an inoculum concentration of approximately 1 x 10(4) cells/mL. In one group, long-term cultured F. pedrosoi cells were inoculated in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fonsecaea pedrosoi; Experimental chromoblastomycosis; Sclerotic cells.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400007

Autologous or heterologous fibrin sealant scaffold: which is the better choice?
Provedor de dados: 80
Autores: Ferreira,Rui Seabra.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Fibrin sealant; Snake venom; Scaffold; Stem cell.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200103

Effect of transforming growth factor beta on the functional activity of human monocytes "in vitro" infected with Paracoccidioides brasiliensis
Provedor de dados: 80
Autores: Martins,R. A. R.
Tipo: Info:eu-repo/semantics/other
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100015

Alternagin-C binding to α2β1 integrin controls matrix metalloprotease-9 and matrix metalloprotease-2 in breast tumor cells and endothelial cells
Provedor de dados: 80
Autores: Moritz,Milene Nóbrega de Oliveira; Eustáquio,Lívia Mara Santos; Micocci,Kelli Cristina; Nunes,Ana Carolina Caetano; Santos,Patty Karina dos; Vieira,Tamires de Castro; Selistre-de-Araujo,Heloísa Sobreiro.
Abstract  Background Matrix metalloproteinases (MMPs) are key players in tumor progression, helping tumor cells to modify their microenvironment, which allows cell migration to secondary sites. The role of integrins, adhesion receptors that connect cells to the extracellular matrix, in MMP expression and activity has been previously suggested. However, the mechanisms by which integrins control MMP expression are not completely understood. Particularly, the role of α2β1 integrin, one of the major collagen I receptors, in MMP activity and expression has not been studied. Alternagin-C (ALT-C), a glutamate-cysteine-aspartate-disintegrin from Bothrops alternatus venom, has high affinity for an α2β1 integrin. Herein, we used ALT-C as a α2β1 integrin ligand to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ALT-C; Α2β1; Integrin; Cancer; Tumor microenvironment; MMP; C-Myc.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100307

Intestinal microbiota in patients with bacterial infections of the respiratory tract treated with amoxicillin
Provedor de dados: 80
Autores: Monreal,M. T. F. D.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100015

Classification of journals in the QUALIS system of CAPES: URGENT need of changing the criteria!
Provedor de dados: 80
Autores: Andriolo,A; Souza,AFM; Farias,AQ; Barbosa,AJA; França Netto,AS; Hernandez,AJ.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300001

Posters
Provedor de dados: 80
Tipo: Info:eu-repo/semantics/other
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200017

Clostridial toxins: potent poisons, potent medicines
Provedor de dados: 80
Autores: Baldassi,L.
Clostridium is an anaerobic bacterial genus. The clostridia produce more protein toxins than any other bacterial genus and are a rich reservoir of toxins for research and medicinal uses. Clostridia are widely spread in the environment: soil, dust and water, presenting more than 120 described species, although few can cause diseases. Diseases can grossly be divided into neurotropic disorders (nervous system is primarily affected), enterotoxemias (affecting intestinal tract and parenchymatous organs), and gas gangrene (myonecrosis with toxemia). Undoubtedly the most widely recognized infection due to anaerobes was clostridial myonecrosis, but recently interest has arisen for the role of clostridia in intestinal diseases. This report describes the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridiosis; Clostridium spp; Toxins.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400002

Biodistribution studies of bee venom and spider toxin using radiotracers
Provedor de dados: 80
Autores: Yonamine,C. M.; Costa,H.; Silva,J. A. A.; Muramoto,E.; Rogero,J. R.; Troncone,L. R. P.; Camillo,M. A. P.
The use of radiotracers allows the understanding of the bioavailability process, biodistribution, and kinetics of any molecule labelled with an isotope, which does not alter the molecule's biological properties. In this work, technetium-99m and iodine-125 were chosen as radiotracers for biodistribution studies in mice using bee (Apis mellifera) venom and a toxin (PnTX2-6) from the Brazilian "armed" spider (Phoneutria nigriventer) venom. Incorporated radioactivity was measured in the blood, brain, heart, lung, liver, kidney, adrenal gland, spleen, stomach, testicle, intestine, muscle, and thyroid gland. Results provided the blood kinetic parameter, and different organs distribution rates.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phoneutria nigriventer; Apis mellifera; Toxin; Venom; Radiotracer; Biodistribution.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100006

Hospital infection in a school of medicine intensive care unit: patient evaluation according to ward of origin
Provedor de dados: 80
Autores: Olbrich,S. R. L. R.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100011

Histological and biochemical effects induced by sublethal doses of Bothrops jararacussu venom in mice
Provedor de dados: 80
Autores: Zeni,A. L. B.; Becker,A.; Krug,M.; Albuquerque,C. A. C.
Snake venom is characterized by hemorrhagic, coagulant, proteolytic and myotoxic activities which in Bothrops jaracussu venom are related to intraspecific variations. In the present study, female Swiss mice were divided into two groups: treated with 25µg or 50µg venom. These were subdivided into three groups of six animals each, according to blood collection: 2, 4 or 24h after venom injection. Animals were anesthetized using diethyl-ether inhalation and 1ml of blood was collected by heart puncture. Then, the following organs were removed: spleen, skeletal muscle, kidneys, liver and lungs; histological sections were obtained and stained with hematoxylin-eosin (HE). The following biochemical parameters were analyzed: aspartate aminotransferase (AST/GOT),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararacussu; Snake venoms; Sublethal doses; In vivo studies; Biochemical parameters; Histopathological alterations.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300009

Candidemia in a brazilian tertiary hospital: microbiological and clinical features over a six-year period
Provedor de dados: 80
Autores: Mondelli,AL; Niéro-Melo,L; Bagagli,E; Camargo,CH; Bruder-Nascimento,A; Sugizaki,MF; Carneiro,MV; Villas Boas,PJF.
Yeasts are becoming a common cause of nosocomial fungal infections in immunocompromised patients. Such infections often develop into sepsis with high mortality rates. The aim of this study was to evaluate some of the numerous factors associated with the development of candidemia. Medical records were retrospectively analyzed of 98 Candida spp. patients. Results showed that the most prevalent risk factors for developing candidemia were: antibiotics and antifungal agents (93.9% and 79.6%, respectively); the use of central venous catheter (93.9%); mechanical ventilation (73.5%); and parenteral nutrition (60.2%). The main species of Candida found were: C. parapsilosis (37.76%), C. albicans (33.67%); and others (28.57%). C. glabrata showed the highest mortality...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candidemia; Mortality; Associated factors; Candida albicans.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200015

Characterization of the allergen Sol gem 2 from the fire ant venom, Solenopsis geminata
Provedor de dados: 80
Autores: Sukprasert,S; Uawonggul,N; Jamjanya,T; Thammasirirak,S; Daduang,J; Daduang,S.
Sol i 2 is a potent allergen in Solenopsis invicta venom, and most humans exhibit reactivity to it. The Sol gem 2 allergen found in the venom of the Thai tropical fire ant Solenopsis geminata was analysed in the present study. The protein was present in higher amounts than other proteins, as determined by SDS-PAGE, and presumably has allergenic properties similar to those of Sol i 2. Sol gem 2 molecular weight is 28 and 15 kDa, respectively, under non-reducing and reducing conditions, indicating that its native form is a dimer. LC-MS/MS analysis confirmed its similarity to Sol i 2. The mono/dimeric form of Sol gem 2 was determined to be relevant by proteomic approach and immunoblotting. An anti-Sol gem 2 antibody was produced in mice, with a titer greater...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allergy; Ant venom; Hymenoptera; Immunoreactivity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300010

Lymphoscintigraphic evaluation of paracoccidioidomycosis patients
Provedor de dados: 80
Autores: Griva,B. L.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300016

Isolation of biologically active peptides from the venom of Japanese carpenter bee, Xylocopa appendiculata
Provedor de dados: 80
Autores: Kawakami,Hiroko; Goto,Shin G.; Murata,Kazuya; Matsuda,Hideaki; Shigeri,Yasushi; Imura,Tomohiro; Inagaki,Hidetoshi; Shinada,Tetsuro.
Abstract  Background  Mass spectrometry-guided venom peptide profiling is a powerful tool to explore novel substances from venomous animals in a highly sensitive manner. In this study, this peptide profiling approach is successfully applied to explore the venom peptides of a Japanese solitary carpenter bee, Xylocopa appendiculata (Hymenoptera: Apoidea: Apidae: Anthophila: Xylocopinae: Xylocopini). Although interesting biological effects of the crude venom of carpenter bees have been reported, the structure and biological function of the venom peptides have not been elucidated yet.  Methods  The venom peptide profiling of the crude venom of X. appendiculata was performed by matrix-assisted laser desorption/ionization-time of flight mass spectroscopy. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xylocopa appendiculata; Carpenter bee; Venom peptides; Solitary bee; Mass spectrometry analysis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100312

The impact of multimedia on teaching tropical medicine
Provedor de dados: 80
Autores: Sarmento e Souza,M. F.; Ferreira,A. S. S. B.; Martinez,J. C.; Barraviera,B.
This study evaluated the impact of multimedia in the information transfer of subjects specific to Tropical Diseases - Tetanus and Snake Envenoming. We evaluated the autonomous learning process of 76 fourth-year medical students at Botucatu School of Medicine of UNESP, using printed matter, video, and CD-ROM. The students were submitted to a specific test, which was repeated approximately one week later. They were divided into groups and received a kit containing a textbook, a video, and a CD-ROM. These materials were used for out-of-class study. Before the second test, the students gave a seminar,where they discussed and resolved their doubts with their professor. The results of the first test showed averagesbetween 4.27±1.41 and 6.41±1.61. The second...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medical education; Tropical diseases; Multimedia; Video; Printed matter.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200007

Embriotoxic effects of maternal exposure to Tityus serrulatus scorpion venom
Provedor de dados: 80
Autores: Barão,A. A. S.; Nencioni,A. L. A.; Dorce,V. A. C.
Tityus serrulatus is the most venomous scorpion in Brazil; however, it is not known whether its venom causes any harm to the offspring whose mothers have received it. This study investigates whether the venom of T. serrulatus may lead to deleterious effects in the offspring, when once administered to pregnant rats at a dose that causes moderate envenomation (3mg/kg). The venom effects were studied on the 5th and on the 10th gestation day (GD5 and GD10). The maternal reproductive parameters of the group that received the venom on GD5 showed no alteration. The group that received the venom on GD10 presented an increase in post-implantation losses. In this group, an increase in the liver weight was also observed and one-third of the fetuses presented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venom; Perinatal; Tityus serrulatus; Pregnancy; Embryofetotoxicity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200010

Serum levels of tnf-alpha, inf-gamma, il-12, and il-10 in patients with tuberculosis and other bacterial infections
Provedor de dados: 80
Autores: Aragão,V. D. N.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100014

Genotyping of Brazilian Giardia duodenalis human axenic isolates
Provedor de dados: 80
Autores: Coradi,ST; David,EB; Oliveira-Sequeira,TCG; Ribolla,PEM; Carvalho,TB; Guimarães,S.
Giardia duodenalis is a complex species that comprises at least seven distinct genetic groups (A to G), but only genotypes A and B are known to infect humans and a wide variety of other mammals. Regardless of biological, biochemical and antigenic analysis, several isolates maintained in vitro were not genetically typed yet. So, in the present study, five Brazilian axenic isolates obtained from asymptomatic and symptomatic patients were typed in order to determine the major genetic groups to which the isolates belonged. DNA was extracted from axenic trophozoites, fragments of glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi) genes were amplified by PCR and the isolate genotyping was carried out using restriction fragment length polymorphism...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Giardia duodenalis; Axenic isolates; Molecular typing; Genotype.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300016

The WHO strategy for prevention and control of snakebite envenoming: a sub-Saharan Africa plan
Provedor de dados: 80
Autores: Chippaux,Jean-Philippe; Massougbodji,Achille; Habib,Abdulrazaq G.
Abstract Snakebite is a critical public health issue in tropical countries, particularly in Africa, where 20% of snakebites globally occur. In 2017, the WHO added snakebite envenoming to the category A of neglected tropical diseases. In 2019, thanks to broad institutional and international NGO support, including strong mobilization of African experts and governments, WHO launched a strategy for prevention and control of snakebite envenoming with more ambitious goals. In sub-Saharan Africa, accessibility of antivenoms and symptomatic, adjuvant or replacement therapy is a priority. Several antivenoms are available but their evaluation has not been properly carried out and they remain expensive. To date, there are no manufacturers of antivenom in sub-Saharan...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Snakebite; Envenomation; Antivenom; Sub-Saharan Africa; Neglected tropical diseases; Control.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100101

General characterization of venom from the Moroccan snakes Macrovipera mauritanica and Cerastes cerastes
Provedor de dados: 80
Autores: Oukkache,N; Lalaoui,M; Ghalim,N.
Ophidian envenomation accidents constitute a serious public health problem in many countries around the globe. Over 5 million such accident cases occur each year causing more than 100,000 deaths. In Africa, more than 20,000 deaths per year are registered while 400,000 envenomation victims retain severe and permanent functional sequelae. In Morocco, snakebites are frequent and of greater severity in children. They occur mostly in rural areas. The incidence of these bites remains poorly understood and vastly underestimated. The epidemiological data are not well known due to the absence of a national registry, whereas a significant proportion of envenomations receive only traditional treatment methods in non-medical intensive care. This prompted us to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerastes cerastes; Macrovipera mauritanica; Snake venom; Characterization; Biological activities; Cross-reaction.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400009

Lipid profile and body composition of HIV-1 infected patients treated with highly active antiretroviral therapy
Provedor de dados: 80
Autores: Pontes,L. C. R.; Carvalho,L. R.; Souza,L. R.; Trindade,E. B. S. M.; Pereira,P. C. M.
Highly active antiretroviral therapy (HAART) has been associated with the development of a clinical group and metabolic disorders such as peripheral lipodystrophy syndrome in AIDS. The aim of this study was to analyze the lipid profile, the clinical aspects, and the body composition of HIV-1 infected individuals treated with or without protease inhibitor (PI) during the highly active antiretroviral therapy. In total, 62 individuals were evaluated in this study; 15 healthy individuals (Control Group: CG), 11 HIV-1 infected individuals treated without antiretroviral therapy (Group 1: G1), 14 HIV-1 infected individuals treated with antiretroviral therapy plus protease inhibitor (Group 2: G2), and 22 HIV-1 infected individuals treated with antiretroviral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipodystrophy; HIV-1; Highly active antiretroviral therapy; Dyslipidemia; Hypertriglyceridemia.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200006

Microbiological evaluation of oysters sold in the greater Recife, PE. area
Provedor de dados: 80
Autores: Mendes,E. S.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100010

Standardization and validation of Dot-ELISA assay for Paracoccidioides brasiliensis antibody detection
Provedor de dados: 80
Autores: Kamikawa,Camila Mika; Mendes,Rinaldo Poncio; Vicentini,Adriana Pardini.
Abstract  Background  Paracoccidioidomycosis (PCM) is a neglected systemic mycosis caused by a dimorphic fungus of the Paracoccidioides genus. The standard diagnosis is based on isolation of the fungi in culture, and by microscopic visualization of characteristic multiple budding yeast cells in biological samples. However, in some situations, access to the site of injury prevents the collection of biological material. A variety of immuno-serological techniques has proven useful for allowing inferring diagnosis with a certain degree of certainty, thus optimizing time. The aim of this study was to standardize and validate the Dot-ELISA (DE) assay, comparing it with the serological standard, double immunodiffusion (DI).  Methods  In order to standardize the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paracoccidioidomycosis; Paracoccidioides brasiliensis; Immunodiagnostic tool; Dot-ELISA; Validation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100305

Venomics and antivenomics of the poorly studied Brazil’s lancehead, Bothrops brazili (Hoge, 1954), from the Brazilian State of Pará
Provedor de dados: 80
Autores: Sanz,Libia; Pérez,Alicia; Quesada-Bernat,Sarai; Diniz-Sousa,Rafaela; Calderón,Leonardo A.; Soares,Andreimar M.; Calvete,Juan J.; Caldeira,Cleópatra A. S.
ABSTRACT  Background: The Brazil’s lancehead, Bothrops brazili, is a poorly studied pit viper distributed in lowlands of the equatorial rainforests of southern Colombia, northeastern Peru, eastern Ecuador, southern and southeastern Venezuela, Guyana, Suriname, French Guiana, Brazil, and northern Bolivia. Few studies have been reported on toxins isolated from venom of Ecuadorian and Brazilian B. brazili. The aim of the present study was to elucidate the qualitative and quantitative protein composition of B. brazili venom from Pará (Brazil), and to carry out a comparative antivenomics assessment of the immunoreactivity of the Brazilian antibothropic pentavalent antivenom [soro antibotrópico (SAB) in Portuguese] against the venoms of B. brazili and reference...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops brazili; Venomics; Third-generation antivenomics; Brazilian antibothropic polyvalent antivenom.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100308

Pharmacological characterization of cnidarian extracts from the Caribbean Sea: evaluation of anti-snake venom and antitumor properties
Provedor de dados: 80
Autores: Oliveira,Cláudia S.; Caldeira,Cleópatra A. S.; Diniz-Sousa,Rafaela; Romero,Dolores L.; Marcussi,Silvana; Moura,Laura A.; Fuly,André L.; Carvalho,Cicília de; Cavalcante,Walter L. G.; Gallacci,Márcia; Pai,Maeli Dal; Zuliani,Juliana P.; Calderon,Leonardo A.; Soares,Andreimar M.
Abstract  Background: Cnidarians produce toxins, which are composed of different polypeptides that induce pharmacological effects of biotechnological interest, such as antitumor, antiophidic and anti-clotting activities. This study aimed to evaluate toxicological activities and potential as antitumor and antiophidic agents contained in total extracts from five cnidarians: Millepora alcicornis, Stichodactyla helianthus, Plexaura homomalla, Bartholomea annulata and Condylactis gigantea (total and body wall).  Methods: The cnidarian extracts were evaluated by electrophoresis and for their phospholipase, proteolytic, hemorrhagic, coagulant, fibrinogenolytic, neuromuscular blocking, muscle-damaging, edema-inducing and cytotoxic activities.  Results: All...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caribbean sea cnidarians; Bioprospection; Antiophidic; Antitumor; Natural products.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100313

Purification and functional characterization of two fibrinogenolytic enzymes from Bothrops alternatus venom
Provedor de dados: 80
Autores: Costa,J. O.; Petric,C. B.; Hamaguchi,A.; Homsi-Brandeburgo,M. I.; Oliveira,C. Z.; Soares,A. M.; Oliveira,F.
Two fibrinogenolytic enzymes, Bothrops alternatus metalloprotease isoform (BaltMP)-I and II, were purified from Bothrops alternatus venom using Diethylaminoethyl (DEAE) Sephacel, Sephadex G-75 and Heparin-Agarose column chromatography. Purified BaltMP-I and II ran as single protein bands on analytical polyacrylamide gel electrophoresis and showed molecular weights of 29000 and 36000, respectively, under reducing conditions in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). BaltMP-II, but not BaltMP-I, displayed blood-clotting activity in bovine plasma, which was about 10-fold higher than that of the crude venom. Both enzymes were proteolytically active against bovine fibrinogen as substrate. When fibrinogen and each enzyme were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops alternatus; Metalloproteases; Functional characterization; Fibrinogenolytic activity; Defibrinogenation in vivo.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300007

Trypanosomatids in dogs belonging to individuals with chronic Chagas’ disease living in Botucatu town and surrounding region, São Paulo State, Brazil
Provedor de dados: 80
Autores: Lucheis,S. B.; Da Silva,A. V.; Araújo Jr,J. P.; Langoni,H.; Meira,D. A.; Marcondes-Machado,J.
Among domestic animals, dogs are considered to be the major reservoirs of trypanosomatids and, due to their proximity to man, the presence of these parasites in dogs is an alert to actions aiming at triatomine control. Fifty dogs (26 males and 24 females), aged from 2 months to 15 years, belonging to 30 chronic Chagas’ disease individuals from 15 different municipalities in the western region of São Paulo State, Brazil, were subjected to blood collection for the following tests: artificial xenodiagnosis, blood culture, and Polymerase Chain Reaction (PCR). Forty-three (86%) out of 50 dogs were positive to at least one of the tests performed; 34 (68%) were positive to xenodiagnosis, 30 (60%) to blood culture, and 25 (50%) to PCR for T. cruzi and/or T....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; T. rangeli; Dog; Xenodiagnosis; Blood culture; Polymerase chain reaction.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400009

Evaluation of anti-fungicidal drugs in the treatment of BALB/c mice inculated with Lacazia loboi
Provedor de dados: 80
Autores: Rosa,P. S.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100011

Does the rattle of Crotalus durissus terrificus reveal its dietary history?
Provedor de dados: 80
Autores: Martinez,Melissa Gaste; Ducatti,Carlos; Silva,Evandro Tadeu; Sant’Anna,Savio Stefanini; Sartori,Maria Márcia Pereira; Barraviera,Benedito.
Background Environmental devastation threatens the survival of many species, including venomous snakes such as the South American rattlesnake Crotalus durissus terrificus. This observation is based on the decrease of snakes collected and donated to Brazilian research institutes. Nevertheless, some individuals have managed to survive and procreate. The question is how these snakes are adapting in these new environmental conditions.Methods To answer it, the carbon-13 level of rattlesnakes and their feed (either laboratory or wild mice) was evaluated by isotope-ratio mass spectrometry. Thus, rattle segments from 16 adults and 15 offspring of captive snakes, and of three wild newborn C. d. terrificus were evaluated as well as 17 Mus musculus mice captured in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food; Carbon-13; Crotalus durissus terrificus; Stable isotopes.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200342

The international view of envenoming in Brazil: myths and realities
Provedor de dados: 80
Autores: Bochner,Rosany.
Being distant from Brazil's great natural diversity, from its long tradition in the study of snakebites and from the fact that it is one of the few countries which has a national information system for monitoring incidents involving venomous animals, non-Brazilian researchers face risks when estimating the incidence of these accidents in the country. The present work offers a critical review of the main estimates undertaken since 1954. It is interesting to note contradictions between textual and graphic information within the same article, variations over time in the work of a same researcher and differences among distinct authors, and that all these issues remain unmentioned or undiscussed. Comparison among such estimates and the data available at the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Incidence; Snakebites; Scorpionism; Epidemiology; Information systems.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100205

Hymenoptera venom review focusing on Apis mellifera
Provedor de dados: 80
Autores: Lima,P. R. de; Brochetto-Braga,M. R.
Hymenoptera venoms are complex mixtures containing simple organic molecules, proteins, peptides, and other bioactive elements. Several of these components have been isolated and characterized, and their primary structures determined by biochemical techniques. These compounds are responsible for many toxic or allergic reactions in different organisms, such as local pain, inflammation, itching, irritation, and moderate or severe allergic reactions. The most extensively characterized Hymenoptera venoms are bee venoms, mainly from the Apis genus and also from social wasps and ant species. However, there is little information about other Hymenoptera groups. The Apis venom presents high molecular weight molecules - enzymes with a molecular weight higher than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hymenoptera; Venom; Enzymes; Peptides.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200002

Immediate radical fang mark ablation may allow treatment of japanese viper bite without antivenom
Provedor de dados: 80
Autores: Fujioka,M; Oka,K; Kitamura,R; Yakabe,A; Ito,M.
Administration of antivenom is currently the standard treatment for snake envenomation. However, it can sometimes cause anaphylactic reactions including urticaria, bronchospasm and hypotension. Furthermore, it may also provoke life-threatening complications, even though the mortality rate is less than 1%. In this study, we present a new treatment - immediate radical fang mark ablation - that was successfully performed on five victims of Japanese viper bites without antivenom use. In these five victims of venomous snakebites, surgical debridement was immediately performed. Two patients received a free-skin graft to resurface their wounds while three of them healed conservatively (i.e. by ointment treatment without surgery). After treatment, all patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ablation; Viper bite; Antivenom; Fang mark; Envenomation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100016

Scorpion incidents, misidentification cases and possible implications for the final interpretation of results
Provedor de dados: 80
Autores: Lourenço,Wilson R.
Abstract The aim of this contribution is to bring general information on the classification and in particular on the specific identification of scorpion species dangerous to humans. Several generic groups are taken into consideration, but the Neotropical genus Tityus C. L. Koch, 1836 is used as a major example. The content of this paper is mostly addressed to non-specialists whose research embraces scorpions in several fields such as venom toxins and public health. Although efforts have been made in the last 20 years to create better links between ‘true scorpion experts’ and non-specialists who use scorpions in their research, such exchanges had never led to a consensus among those different branches of biological and medical research. Consequently, many...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion; Dangerous species; Misidentification; Biased results; Buthidae; Tityus.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100202

Neuroprotective properties of RT10, a fraction isolated from Parawixia bistriata spider venom, against excitotoxicity injury in neuron-glia cultures
Provedor de dados: 80
Autores: Primini,Eduardo Octaviano; Liberato,José Luiz; Fontana,Andreia Cristina Karklin; Santos,Wagner Ferreira dos.
Abstract  Background: L-Glutamate (L-Glu), the major excitatory neurotransmitter in the mammalian Central Nervous System (CNS), is essential to cognitive functions. However, when L-Glu is accumulated in large concentrations at the synaptic cleft, it can induce excitotoxicity that results in secondary damage implicated in many neurological disorders. Current therapies for the treatment of neurological disorders are ineffective and have side effects associated with their use; therefore, there is a need to develop novel treatments. In this regard, previous studies have shown that neuroactive compounds obtained from the venom of the spider Parawixia bistriata have neuroprotective effects in vitro and in vivo. In this sense, this work aimed to evaluate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-Glutamate; Excitotoxicity; Neuroprotection; Parawixia bistriata; RT10.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100311

First serine protease inhibitor isolated from Rhinella schneideri poison
Provedor de dados: 80
Autores: Shibao,Priscila Y T; Anjolette,Fernando A P; Lopes,Norberto P.; Arantes,Eliane C.
Background Toad secretions are a source of molecules with potential biotechnological application on a wide spectrum of diseases. Toads from theRhinella family have two kinds of poisonous glands, namely granular and mucous glands. Rhinella schneideritoads produce granular secretions that comprise a great number of molecules, including serine proteases inhibitors. Serine proteases, such as trypsin, chymotrypsin and elastase, are enzymes that have a serine amino acid into its catalytic site and can be found in a large number of vertebrate species and pathogenic microorganisms. Therefore, the present work aims to purify a serine protease inhibitor from Rhinella schneiderigranular secretions.Findings This study presents the protocol used to purify a serine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bufadienolide; Rhinella schneideri; Serine protease inhibitor.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100501

Correlation between cytokine serum levels, number of CD4+ T cells/mm³ and viral load in HIV-1 infected individuals with or without antiretroviral therapy
Provedor de dados: 80
Autores: Meira,D. A.; Souza,L. R.; Calvi,S. A.; Lima,C. R. G.; Henriques,R. M. S.; Pardini,M. I.; Silva,V. A.; Iuan,F. C.; Marcondes-Machado,J.
Seventy-nine HIV-1 infected patients were studied in three groups: Group G1 - 11 patients with no antiretroviral therapy; G2 - 40 patients undergoing antiretroviral therapy, 33 with only two nucleoside reverse transcriptase inhibitors (NRTI), and seven with two NRTI and one protease inhibitor (PI), all with viral load (VL) equal or higher than 80 copies of plasma RNA/ml; Group G3 - 28 patients, 23 on highly active antiretroviral therapy (HAART), 18 with two NRTI and one PI, and five with two NRTI and one non-nucleoside reverse transcriptase inhibitor (NNRTI), the remaining five with combination of two NRTI. All G3 patients had undetectable viral load for at least the past six months. The control group (Gc) included 20 normal blood donors without clinical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serum cytokines; HIV-1/AIDS; HAART.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300008

Scorpion envenomation in pygmies from Democratic Republic of Congo, the example of Pelenge Center, Lomela, DRC
Provedor de dados: 80
Autores: Biezakala Mudiandambu,E; Odio Wobin,T; Kabele Ngiefu,C; Tati Kinkela,R; Mbanzulu Makola,K.
In a survey among the pygmies of central Democratic Republic of Congo, the incidence of scorpion stings seemed very high with a severity greater than expected. Species responsible were not identified. Specific studies are needed to clarify the risk emerging in the equatorial African forest.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Arothron hispidus; Tetrodotoxin; Bioassay; ATPase and AchE enzymatic activities; Anticancer activity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400015

Hematological and plasma biochemical parameters in a wild population of Naja naja (Linnaeus, 1758) in Sri Lanka
Provedor de dados: 80
Autores: Dissanayake,Duminda S. B.; Thewarage,Lasanthika D.; Rathnayake,Rathnayake M. P. Manel; Kularatne,Senanayake A. M.; Ranasinghe,Jamburagoda G. Shirani; Rajapakse,Rajapakse P. V. Jayantha.
Abstract  Background  Hematological studies of any animal species comprise an important diagnostic method in veterinary medicine and an essential tool for the conservation of species. In Sri Lanka, this essential technique has been ignored in studies of many species including reptiles. The aim of the present work was to establish a reference range of hematological values and morphological characterization of wild spectacled cobras (Naja naja) in Sri Lanka in order to provide a diagnostic tool in the assessment of health condition in reptiles and to diagnose diseases in wild populations.  Methods  Blood samples were collected from the ventral caudal vein of 30 wild-caught Naja naja (18 males and 12 females). Hematological analyses were performed using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematology; Blood cells; Plasma biochemistry; Naja naja.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100303

Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response
Provedor de dados: 80
Autores: Pereira,Andreia Vieira; Barros,Gustavo de; Pinto,Erika Gracielle; Tempone,Andre Gustavo; Orsi,Ricardo de Oliveira; Santos,Lucilene Delazari dos; Calvi,Sueli; Ferreira Jr,Rui Seabra; Pimenta,Daniel Carvalho; Barraviera,Benedito.
Abstract  Background Apis mellifera venom, which has already been recommended as an alternative anti-inflammatory treatment, may be also considered an important source of candidate molecules for biotechnological and biomedical uses, such as the treatment of parasitic diseases.  Methods  Africanized honeybee venom from Apis mellifera was fractionated by RP-C18-HPLC and the obtained melittin was incubated with promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Cytotoxicity to mice peritoneal macrophages was evaluated through mitochondrial oxidative activity. The production of anti- and pro-inflammatory cytokines, NO and H2O2 by macrophages was determined.  Results  Promastigotes and intracellular amastigotes were susceptible to melittin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melittin; Apis mellifera; Leishmania; Leishmaniasis; Peptides; Toxins; Antiparasitic; Cytokines.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100301

A therapeutic study comparing the association of rifampin, ofloxacin and minocycline with the association of rifampin, clofazimine and dapsone in multibacillary leprosy patients
Provedor de dados: 80
Autores: Somei,Ura.
Tipo: Info:eu-repo/semantics/article
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100011

Importance of the association of molecular and immunological diagnosis in immunocompetent patient with Histoplasma capsulatum and Cryptoccocus neoformans infection: a case report
Provedor de dados: 80
Autores: Dantas,Kátia Cristina; Freitas,Roseli Santos de; Garcia,Roberta Scholz Pinto; Silva,Marcos Vinícius da; Muricy,Edna Cleide Mendes; Kohara,Valdelene Sayuri; Vicentini,Adriana Pardini.
This case reports an immunocompetent 29-year-old woman with suspected pneumonia, suggestive of fungal infection. Immunoblotting analysis reactivity againstHistoplasma capsulatum and Paracoccidioides brasiliensis were observed. Nested-PCR in blood employing species-specific primers was positive for H. capsulatum andCryptococcus neoformans. The evaluation of paucisymptomatic patients with positive results for H. capsulatum and C. neoformans could be relevant for the prevention as well as the possible evaluation of the reactivated quiescent foci. In conclusion, the associated methodology may have contributed to the monitoring endogenous reactivation of these diseases.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Immunocompetent individuals; Nested PCR; Immunoblotting; Fungal infections; Blood fluid.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200507

Unusual fatal multiple-organ dysfunction and pancreatitis induced by a single wasp sting
Provedor de dados: 80
Autores: Azad,C; Parmar,VR; Jat,KR.
Acute onset of multiple organ dysfunction syndrome (MODS) is a well-known complication following multiple wasp stings. However, MODS after a single wasp sting has been rarely reported in children and acute pancreatitis have probably never been observed before. Herein we describe the case of a 12-year-old boy who had urticaria and abdominal pain after a single wasp sting. The child gradually developed MODS while his abdominal complaints were worsening. Despite aggressive supportive management, the child did not survive. Afterward, the cause of the acute abdomen was finally diagnosed as acute pancreatitis. Both MODS and pancreatitis following a single wasp sting are very unusual. Thus, although pancreatitis is rarely manifested, it should be suspected after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wasp sting; Multiple organ dysfunction syndrome; Pancreatitis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100015

Scintigraphy of the hepatobiliar system in patients with pulmonary tuberculosis
Provedor de dados: 80
Autores: Camargo,R. S. A.; Silva,E. T.; Griva,B. L.; Pereira,P. C. M.
The objective of this paper was to evaluate the hepatobiliary function of patients with pulmonary tuberculosis under triple treatment, using the technetium-99m-DISIDA (99mTc-DISIDA) hepatobiliary scintigraphy. Ten men and three women with pulmonary tuberculosis were subjected to hepatobiliary scintigraphy at the beginning of triple treatment (M1) and two months after it (M2). Patients were from the urban area, of low socioeconomic level, malnourished, and chronic alcohol and/or tobacco users. Ten normal individuals were evaluated as controls. Radiotracer images were acquired on a computerized gamma camera (Orbiter-Siemens) and T1/2 uptake and excretion values were calculated. Nutritional status and serum hepatic enzyme levels for each patient were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulmonar tuberculosis; Scintigraphy; Hepatobiliar system; Antituberculosis drugs.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200006

Proteomic characterization of the thermostable toxins from Naja naja venom
Provedor de dados: 80
Autores: Binh,DV; Thanh,TT; Chi,PV.
Naja naja snake venom presents abundant thermostable peptides. Many of them possess useful pharmacological activity that may be employed for drug development. For the proteomic characterization of such toxins, in the present study, Naja naja venom solution was heated up to 100°C for 10, 30, 60, 120, 180 and 300 minutes and protein fractions of non-heated and heated venom were analyzed by two-dimensional nano-liquid chromatography coupled online with tandem mass spectrometry. After heating for 300 minutes, a total of 32 peptides were still detected in the supernatant. The identified peptides belong to the following groups: cardiotoxins, neurotoxins and cytotoxins. It was found that thermostable peptides are able to preserve their analgesic activity after a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Naja naja; Snake venom proteome; Thermostable peptides; Mass spectrometry; Analgesic activity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400014

Perspective for pharmaceutical innovation in Brazil - center for applied toxinology (CEPID- center for research, innovation and dissemination - FAPESP)
Provedor de dados: 80
Autores: Camargo,Antonio Carlos Martins de.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400001

Oxidative stress and hepatotoxicity in rats induced by poisonous pufferfish (Lagocephalus lagocephalus) meat
Provedor de dados: 80
Autores: Saoudi,M; Abdelmouleh,A; Ellouze,F; Jamoussi,K; El Feki,A.
This study was undertaken to evaluate the effect of pufferfish (Lagocephalus lagocephalus) meat poisoning on hepatic functions of Wistar rats. For this purpose, groups of rats (Lcr, Lcu+b and Lcu-b) received diet supplemented with 10% of raw or cooked meat, respectively, with or without cooking water of L. lagocephalus while groups Mcr and Mcu+b received diet supplemented with 10% of raw or cooked meat of Liza aurata, which were used as a negative control. In Lcu+b group, ALT, AST and ALP rates (hepatic enzyme markers) decreased after two months of treatment, indicating liver damage. We also observed an increase of 54 and 65% of thiobarbituric acid reactive substances (TBARS) in their livers respectively 48 hours and two months after treatment compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatotoxicity; Lagocephalus lagocephalus; Oxidative stress; Tetrodotoxin.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300006

True or false coral snake: is it worth the risk? A Micrurus corallinus case report
Provedor de dados: 80
Autores: Strauch,Marcelo Abrahão; Souza,Guilherme Jones; Pereira,Jordana Nahar; Ramos,Tyelli dos Santos; Cesar,Marcelo Oliveira; Tomaz,Marcelo Amorim; Monteiro-Machado,Marcos; Patrão-Neto,Fernando Chagas; Melo,Paulo A.
Abstract  Background Bites provoked by the genus Micrurus represent less than 1% of snakebite cases notified in Brazil, a tiny fraction compared with other genus such as Bothrops and Crotalus, which together represent almost 80% of accidents. In addition to their less aggressive behavior, habits and morphology of coral snakes are determinant factors for such low incidence of accidents. Although Micrurus bites are rare, victims must be rescued and hospitalized in a short period of time, because this type of envenoming may evolve to a progressive muscle weakness and acute respiratory failure.  Case Presentation We report an accident caused by Micrurus corallinus involving a 28-year-old Caucasian sailor man bitten on the hand. The accident occurred in a...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Coral snake; Envenoming; Micrurus spp.; Snakebites; Ophidism.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100501

Hepatozoon spp. (Hepatozoidae) prevalence in snakes: Morphological, morphometrical and molecular characterization in Crotalus durissus terrificus (Viperidae) naturally infected
Provedor de dados: 80
Autores: Moço,T. C.
Tipo: Info:eu-repo/semantics/article
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300017

Biodegradation of [D-Leu1] microcystin-LR by a bacterium isolated from sediment of Patos Lagoon estuary, Brazil
Provedor de dados: 80
Autores: Lemes,Gilmar AF; Kist,Luiza W; Bogo,Mauricio R; Yunes,João S.
Background Toxic cyanobacterial blooms are recurrent in Patos Lagoon, in southern Brazil. Among cyanotoxins, [D-Leu1] microcystin-LR is the predominant variant whose natural cycle involves water and sediment compartments. This study aimed to identify and isolate from sediment a bacterial strain capable of growing on [D-Leu1] microcystin-LR. Sediment and water samples were collected at two distinct aquatic spots: close to the Oceanographic Museum (P1), in Rio Grande City, and on São Lourenço Beach (P2), in São Lourenço do Sul City, southern Brazil. Methods [D-Leu1] microcystin-LR was isolated and purified from batch cultures of Microcystis aeruginosastrain RST9501. Samples of water and sediment from Rio Grande and São Lourenço do Sul were collected....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodegradation; Microcystin; Sediment; Patos Lagoon; Estuary; Brazil.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100313

Use of immunoblotting assay improves the sensitivity of paracoccidioidomycosis diagnosis
Provedor de dados: 80
Autores: Silva,D. F.; Assis,C. M.; Zamboni,I. M.; Barreto,L. C.; Kohara,V. S.; Vicentini-Moreira,A. P.
The purpose of this work was to evaluate two serological assays: double immunodiffusion (DI) and immunoblotting (IB) in immunodiagnosis of paracoccidioidomycosis (PCM). We evaluated by IB assay 23 sera samples from patients with clinical confirmation of PCM, all of them with negative DI results against culture filtrate from Paracoccidioides brasiliensis isolate 113. For IB, as well as for comparative DI assay, we employed soluble components of the cell wall outer surface (SCCWOS) from P. brasiliensis isolate 113 cultivated at 36°C in Fava-Neto's agar medium for 5 and 10 days. Among the 20 sera samples analyzed by DI, 13 (65%) were negative and 7 (35%) were positive against SCCWOS obtained on the 5th and 10th days. By IB assay, 95.4% and 100% of sera...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paracoccidioidomycosis; Immunodiffusion; Immunoblotting.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200009

African Society of Toxinology: a new opportunity for integrating the control of envenomations in Africa
Provedor de dados: 80
Autores: Chippaux,JP.
Tipo: Info:eu-repo/semantics/other
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400002