Consulta: subjectFacets:"Α-amylase"
Registros recuperados: 12
Data/hora: 03/06/2024 18:31:03

Efficacy and Metabolizable Energy Equivalence of an α-Amylase-β-Glucanase Complex for Broilers
Provedor de dados: 89
Autores: Vieira,SL; Stefanello,C; Rios,HV; Serafini,N; Hermes,RG; Sorbara,JOB.
A trial was conducted to evaluate the effects of adding an exogenous α-amylase-β-glucanase complex produced from Bacillus amyloliquefaciens on the growth performance, carcass yield, and relative AME bioequivalence in broilers fed corn-soy diets from 1 to 40 d of age. One thousand seven hundred and fifty one-day-old Cobb x Cobb 500 slow-feathering male broilers were randomly allotted to seven treatments with 10 replicates of 25 birds each as follows: control diet (C); C diet with reduction of 60 (C-60), 90 (C-90), or 120 (C-120) kcal AME/kg; C diet with reduction of 120 kcal AME/kg and supplemented with 200 (C-120-200), 300 (C-120-300), or 400 (C-120-400) mg of the enzyme complex/kg. Each g of the enzyme complex corresponded to 200 kilo-Novo α-amylase and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α-amylase; Β-glucanase; Broiler; Carbohydrases; Enzyme.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000200227

Aqueous extract of Psidium guajava leaves: phenolic compounds and inhibitory potential on digestive enzymes
Provedor de dados: 42
Autores: SIMÃO,ANDERSON A.; MARQUES,TAMARA R.; MARCUSSI,SILVANA; CORRÊA,ANGELITA D.
ABSTRACT Leaves of Psidium guajava L. (guava) have been widely used in the popular way for prevention and treatment of various diseases. Thus, the objective of this study was to evaluate the inhibitory potential of leaves aqueous extract from three cultivars of P. guajava (Pedro Sato, Paluma and Século XXI) on α-amylase, α-glycosidase, lipase, and trypsin enzymes, in the presence or not of simulated gastric fluid and to determine the content of phenolic compounds by high performance liquid chromatography. All cultivars presented the same composition in phenolic compounds, but in different proportions. The compounds identified are gallic acid, epigallocatechin gallate, syringic acid, o-coumaric acid, resveratrol, quercetin, and catechin (which was the major...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants; Obesity; Α-amylase; Α-glycosidase; Lipase; Trypsin.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502155

Hypoglycemic property of triterpenoid saponin PFS isolated from Polyscias fruticosa leaves
Provedor de dados: 42
Autores: LUYEN,NGUYEN THI; DANG,NGUYEN HAI; BINH,PHUNG THI XUAN; HAI,NGUYEN THI; DAT,NGUYEN TIEN.
Abstract This paper evaluated the inhibitory effect of 3-O-[β-d-glucopyranosyl-(1→4)-β-d-glucuronopyranosyl] oleanolic acid 28-O-β-d-glucopyranosyl ester (PFS), a major saponin isolated from Polyscias fruticosa leaves, on α-amylase and α-glucosidase, and its potential for reducing the postprandial blood glucose level in mice. In enzyme inhibition assays, PFS strongly inhibited porcine pancreas α-amylase and yeast α-glucosidase. Using the Lineweaver–Burk equation, we found that PFS inhibited porcine pancreas α-amylase in a mixed noncompetitive mode, and yeast α-glucosidase via noncompetitive inhibition. In the sucrose tolerance test, PFS at 100 mg/kg body weight significantly decreased the postprandial blood glucose level in mice fed a high-sucrose diet....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyscias fruticosa; Araliaceae; Α-amylase; Α-glucosidase; Hypoglycemia.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000602881

Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
Provedor de dados: 52
Autores: Morais,Glaucia Almeida de; Takaki,Massanori.
Determination of α- and β-amylase activity in the extracts of cotyledons of Phaseolus vulgaris L. cv. cariocawas done using selective inactivation of α-amylase by lowering the pH of the incubation medium or by the use of EDTA as inhibitor or selective inactivation of β-amylase by the use of HgCl2 or by heating to 70ºC in the presence of CaCl2; and still by using the reagent starch azure for specific determination of α-amylase. Results indicated that the methods used were inappropriate in this case, being indicated the determination of total amylase activity.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α-amylase; Β-amylase; Determination; Selective inactivation; Phaseolus vulgaris.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100003

Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology
Provedor de dados: 52
Autores: Stergiou,Panagiota-Yiolanda; Foukis,Athanasios; Theodorou,Leonidas; Papagianni,Maria; Papamichael,Emmanuel.
The aim of this work was to study the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 using optimized nutritional and cultural conditions in a complex yeast medium under aerobic batch fermentation. By applying the conventional "one-variable-at-a-time" approach and the response surface methodology, the effect of four fermentation parameters (type of carbon source, initial culture pH, temperature, and incubation time) on the growth and α-amylase production was evaluated. The production of α-amylase during 60 h of fermentation increased 13-fold under optimized conditions (1% starch, pH 6.0, 30ºC) in comparison to the conventional optimization method. The initial pH value of 6.13 and temperature of 30.3ºC were optimal conditions by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular enzymes; Α-amylase; Kluyveromyces marxianus; Fermentation; RSM.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300016

Screening and Molecular Identification of New Microbial Strains for Production of Enzymes of Biotechnological Interest
Provedor de dados: 52
Autores: Ghazala,Imen; Haddar,Anissa; Romdhane,Molka Ben; Ellouz-Chaanouni,Semia.
ABSTRACT: This research focused on isolation, identification and characterization of new strains of fungi and bacteria, which were able to produce extracellular xylanase, mannanase, pectinase and α-amylase. Fungi isolates were identified on the basis of analyses of 18S gene sequencing and internal transcribed spacer region. The closest phylogenetic neighbors according to 18S gene sequence and ITS region data for the two isolates M1 and SE were Aspergillus fumigatus and Aspergillus sydowii, respectively. I4 was identified as Bacillus mojavensis on the basis of the 16S rRNA gene sequencing and biochemical properties. The enzyme production was evaluated by cultivating the isolated microorganisms in liquid-state bioprocess using wheat bran as carbon source....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xylanase; Pectinase; Β-mannanase; Α-amylase; Bacillus mojavensis; Aspergillus fumigatus and Aspergillus sydowii.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100510

Starch degradation and nutrition value improvement in corn grits by solid state fermentation technique with Coriolus versicolor
Provedor de dados: 58
Autores: Huang,Mian; Zhang,Song.
The study was conducted to evaluate effect of Coriolus versicolor mycelia on degrading starch and improving nutrition value in corn grits through solid state fermentation technique. The results showed that using soybean meal as a nitrogen source, α-amylase secreted from C. versicolor expressed 407.25U/g of activity, leading to 45.15% of starch degraded. The activity grew with fermentation time until the 15th day, after that the amylase was deactivated rapidly. An orthogonal experiment designed for the study illustrated that degradation rate of starch in corn grits attained to maximum, 50.51%, when 100g of corn grits, added 16g of soybean meal, were fermented by C. versicolor for 12 days, in an initial pH 5.5. After fermenting, compared to the nonfermented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coriolus versicolor; Corn grits; Starch; Solid state fermentation; Α-amylase.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400015

Agro-industrial residues and starch for growth and co-production of polyhydroxyalkanoate copolymer and α-amylase by Bacillus sp. CFR-67
Provedor de dados: 58
Autores: Shamala,T. R.; Vijayendra,S.V.N.; Joshi,G.J.
Polyhydroxyalkanoates (PHA) and α-amylase (α-1,4 glucan-4-glucanohydrolase, E.C. 3.2.1.1) were co-produced by Bacillus sp. CFR-67 using unhydrolysed corn starch as a substrate. Bacterial growth and polymer production were enhanced with the supplementation of hydrolysates of wheat bran (WBH) or rice bran (RBH) individually or in combination (5-20 g L-1, based on weight of soluble substrates-SS). In batch cultivation, a mixture of WBH and RBH (1:1, 10 g L-1 of SS) along with ammonium acetate (1.75 g L-1) and corn starch (30 g L-1) produced maximum quantity of biomass (10 g L-1) and PHA (5.9 g L-1). The polymer thus produced was a copolymer of polyhydroxybutyrate-co-hydroxyvalerate of 95:5 to 90:10 mol%. Presence of WBH and corn starch (10-50 g L-1) in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α-amylase; Bacillus sp.; Corn starch; Polyhydroxyalkanoate; Rice bran hydrolysate; Wheat bran hydrolysate.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300036

Highly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp. AB68
Provedor de dados: 58
Autores: Aygan,Ashabil; Arikan,Burhan; Korkmaz,Hatice; Dinçer,Sadik; Çolak,Ömer.
An alkaliphilic and highly thermostable α-amylase producing Bacillus sp. was isolated from Van soda lake. Enzyme synthesis occurred at temperatures between 25ºC and 40ºC. Analysis of the enzyme by SDS-PAGE revealed a single band which was estimated to be 66 kDa. The enzyme was active in a broad temperature range, between 20ºC and 90ºC, with an optimum at 50ºC; and maximum activity was at pH 10.5. The enzyme was almost completely stable up to 80ºC with a remaining activity over 90% after 30 min pre-incubation. Thermostability was not increased in the presence of Ca2+. An average of 75% and 60ºC of remaining activity was observed when the enzyme was incubated between pH 5 and 9 for 1 h and for 2 h, respectively. The activity of the enzyme was inhibited by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus sp.; Α-amylase; Alkaliphilic; Thermostable; Enzyme.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300027

Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification
Provedor de dados: 58
Autores: Dey,Tapati Bhanja; Banerjee,Rintu.
Polygalacturonase and α-amylase play vital role in fruit juice industry. In the present study, polygalacturonase was produced by Aspergillus awamori Nakazawa MTCC 6652 utilizing apple pomace and mosambi orange (Citrus sinensis var mosambi) peels as solid substrate whereas, α-amylase was produced from A. oryzae (IFO-30103) using wheat bran by solid state fermentation (SSF) process. These carbohydrases were decolourized and purified 8.6-fold, 34.8-fold and 3.5-fold, respectively by activated charcoal powder in a single step with 65.1%, 69.8% and 60% recoveries, respectively. Apple juice was clarified by these decolourized and partially purified enzymes. In presence of 1% polygalacturonase from mosambi peels (9.87 U/mL) and 0.4% α-amylase (899 U/mL), maximum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polygalacturonase; Α-amylase; Activated charcoal; Decolourization; Apple juice clarification.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100014

Independent and synergistic activity of the flavonoids of Gracilaria corticata as promising antidiabetic agents
Provedor de dados: 60
Autores: Sangeetha,Ragupathy.
The therapeutic approaches for Type 2 Diabetes Mellitus rely most on the usage of oral hypoglycaemic drugs. These drugs have adverse side effects and hence alternative medicines are continuously explored. The present study intends to investigate the antidiabetic potential of the flavonoids present in Gracilaria corticata. The flavonoids were isolated (FEGC) and their inhibitory activity on the carbohydrate hydrolysing enzymes such as α-amylase and α-glucosidase was analysed. The flavonoids were found to inhibit α-amylase and α-glucosidase with an IC50 value of 302 µg and 75 µg respectively. The synergistic effect of FEGC and luteolin was also investigated and the results show that both FEGC and luteolin inhibited synergistically at half their IC50 values....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α-amylase; Α-glucosidase; Antidiabetic; Gracilaria corticata; Luteolin; Synergistic.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100618

Enzymatic activity in braúna seeds subjected to thermal stress
Provedor de dados: 65
Autores: Ataíde,Glauciana da Mata; Borges,Eduardo Euclydes de Lima e; Flores,Andressa Vasconcelos.
ABSTRACT: To occur seed germination distinct hydrolytic enzymes work together, enabling primary root protrusion. The objective of this study was to investigate changes in the activities of α-amylase, β-amylase, and glucose-6-phosphate dehydrogenase (G6PdH) during germination of Melanoxylon brauna Schott. seeds under thermal stress. To this end, seeds of this species were germinated under constant temperature (10, 25, 30 or 40°C) and samples were collected every 24h during a 96-h period, in which the activity of the enzymes were evaluated. At 25 and 30°C, optimal temperatures for the germination of the species, the activities of α-amylase, β-amylase, and G6PdH increased with seed imbibitions. At 10 and 40°C, enzyme activities decreased, impairing the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α-amylase; Β-amylase; Glucose-6-phosphate dehydrogenase; Melanoxylon brauna.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000601044