Consulta: subjectFacets:"Acidic enzymatic proteolysis"
Registros recuperados: 2
Data/hora: 08/06/2024 12:31:51

Elements for optimizing a one-step enzymatic bio-refinery process of shrimp cuticles: Focus on enzymatic proteolysis screening
Provedor de dados: 5
Autores: Baron, Regis; Socol, Marius; Kaas, Raymond; Arhaliass, A.; Rodriguez Del Pino, J.; Le Roux, Karine; Donnay-moreno, Claire; Berge, Jean-pascal.
This article complements an earlier work published in 2015 Baron et al. (2015) that showed the interest of a shrimp shells bio-refining process. We compare here the effect of eleven commercial proteases at pH 3.5 or 4.0 on a residual amount of shrimp shells proteins after 6 hours at 50 °C. The two pH are obtained when respectively 40 and 25 mmol of formic acid are added to 5 g of mild dried shell. Deproteinisation yield above 95% are obtained. Residual amino acids profile in the solid phase was identical for the eleven proteases except for pepsin which was similar to the raw material profile. A significant relative increase in the proportion of Glycine is observed for the ten other cases. Likewise, shapes of size exclusion chromatograms of the dissolved...
Tipo: Text
Palavras-chave: Bio-refinery; Shrimp cuticles; Acidic enzymatic proteolysis.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00388/49957/50530.pdf

Kinetic study of solid phase demineralization by weak acids in one-step enzymatic bio-refinery of shrimp cuticles
Provedor de dados: 5
Autores: Baron, Regis; Socol, Marius; Arhaliass, A.; Bruzac, Sandrine; Le Roux, Karine; Del Pino, J. Rodriguez; Berge, Jean-pascal; Kaas, Raymond.
We describe a one-step bio-refinery process for shrimp composites by-products. Its originality lies in a simple rapid (6 h) biotechnological cuticle fragmentation process that recovers all major compounds (chitins, peptides and minerals in particular calcium). The process consists of a controlled exogenous enzymatic proteolysis in a food-grade acidic medium allowing chitin purification (solid phase), and recovery of peptides and minerals (liquid phase). At a pH of between 3.5 and 4, protease activity is effective, and peptides are preserved. Solid phase demineralization kinetics were followed for phosphoric, hydrochloric, acetic, formic and citric acids with pKa ranging from 2.1 to 4.76. Formic acid met the initial aim of (i) 99 % of demineralization yield...
Tipo: Text
Palavras-chave: Bio-refinery; Acidic enzymatic proteolysis; Shrimp cuticles; Demineralization kinetics and modeling.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00281/39244/37809.pdf