Consulta: subjectFacets:"Albumin"
Registros recuperados: 11
Data/hora: 10/06/2024 13:39:09

Influence of the pH of glutaraldehyde and the use of dextran aldehyde on the preparation of cross-linked enzyme aggregates (CLEAs) of lipase from Burkholderia cepacia
Provedor de dados: 69
Autores: Caballero Valdés,Eduardo; Wilson Soto,Lorena; Aroca Arcaya,Germán.
The preparation of cross-linked enzyme aggregates (CLEAs) of lipase has been a challenge due the low amount of lysine residues that lipases have on their surface. The results show that CLEAs prepared using dextran aldehyde (100-200KDa) have a higher hydrolysis activity and particle size (activities between 3186 Â± 21 U/g of CLEA and 4800 Â± 30 U/g of CLEA and particle sizes between 52.6 Â± 18.7 Âµm and 126.2 Â± 53.5 Âµm) than CLEAs prepared with glutaraldehyde (0.1 KDa) (activities between 894 Â± 16 U/g of CLEA and 2874 Â± 20 U/g of CLEA and particle sizes between 21.2 Â± 5.1 Âµm and 83.4 Â± 24.9 Âµm); Thermal stability assays of bioctalysts at 60ÂºC at pH 7.0 using phosphate buffer 25 mM showed that CLEAs prepared with dextran aldehyde have lower residual...
Tipo: Journal article
Palavras-chave: Albumin; Dextran aldehyde; Glutaraldehyde; Immobilisation; Lipase.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300010

Hematological and biochemical responses of pirarucu (Arapaima gigas, Arapaimidae) fed with diets containing a glucomannan product derived from yeast and algae
Provedor de dados: 36
Autores: HOSHINO,Maria Danielle Figueiredo Guimarães; MARINHO,Renata das Graças Barbosa; PEREIRA,Diogle Farias; YOSHIOKA,Eliane Tie Oba; TAVARES-DIAS,Marcos; OZORIO,Rodrigo Otavio de Almeida; RODRIGUEZ,Anselmo Fortunato Ruiz; RIBEIRO,Ricardo Amaral; FARIA,Fernando Sérgio Escócio Drummond Viana de.
ABSTRACT The hematological and biochemical responses of pirarucu fingerlings (Arapaima gigas) fed with diets containing different concentrations of a glucomannan product derived from yeast and algae were evaluated in order to ascertain the effect of these diets on fish physiology. Four treatments were conducted, with three replications, with 12 fish in each tank. The product evaluated (MycosorbA+(r)) was incorporated into the commercial diet, at four concentrations: 0, 1, 2 and 4 g.kg-1, called M0%, M0.1%, M0.2% and M0.4%, respectively. After 45 days of feeding, blood samples from six fish in each replicate were collected to perform the analyses. Their weight and length were determined to calculate the condition factor and weight gain, but no differences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorella vulgaris; Saccharomyces cerevisiae; Leukocytes; Albumin.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000200087

Nitrogen sources associated with different physical forms of corn grain in the diet for steers in feedlot
Provedor de dados: 2
Autores: Porsch, Renata Volpatto; Machado, Diego Soares; Brondani, Ivan Luiz; Cocco, Joziane Michelon; Alves Filho, Dari Celestino; Oliveira, Leonardo Mendes de.
This study evaluated the substitution of soybean meal with non-protein nitrogen sources, in combination with ground or whole corn for feedlot cattle. Fifty-four Charolais x Nellore steers with 22 ± 0.23 months and 250 ± 15.80 kg were assigned to a 3 x 2 factorial completely randomized experimental design. The treatments consisted of different combinations of concentrate formulation: soybean meal with whole or ground corn, conventional urea with whole or ground corn, and protected urea with whole or ground corn. There was interaction between nitrogen source and collection date for serum albumin, with increasing elevation for soybean meal; while there was elevation from day 0 to day 84, stabilizing until the slaughter, for urea. Higher intakes of dry matter...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Albumin; Ingestive behavior; Non-protein nitrogen; Performance.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/42541

Oregano essential oil (Origanum vulgare) to feed laying hens and its effects on animal health
Provedor de dados: 42
Autores: MIGLIORINI,MARCOS J.; BOIAGO,MARCEL M.; ROZA,LENILSON F.; BARRETA,MAURICIO; ARNO,ALESSANDRA; ROBAZZA,WEBER S.; GALVÃO,ALESSANDRO C.; GALLI,GABRIELA M.; MACHADO,GUSTAVO; BALDISSERA,MATHEUS D.; WAGNER,ROGER; STEFANI,LENITA C.M.; SILVA,ALEKSANDRO S. DA.
Abstract Abstract: This study evaluated the effect of oregano essential oil added to the feed of commercial laying hens. This research was focused on the analysis of biochemical changes linked to hepatic function, and protein and lipid metabolism. It was used 240 laying hens (59 weeks-old) distributed in a completely randomized design of six treatments (five repetitions with eight birds each). The experiments were constituted by a control treatment (CT) with the inclusion of zinc bacitracin and five treatments of oregano essential oil (OEO: 0, 50, 100, 150, and 200 mg.kg-1), respectively. After 28 days of feeding, an increase on serum levels of total proteins and globulins was observed on groups T150 and T200, as well as an increase on albumin levels on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Albumin; Cholesterol; Globulin; Total proteins; Triglycerides; Health.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100802

Serum proteinogram of the Campeiro horse
Provedor de dados: 45
Autores: Souza,A.F.; Schade,J.; Ramos,A.F.; Albuquerque,M.S.M.; Fonteque,G.V.; Costa,D.; Muller,T.R.; Fonteque,J.H.
ABSTRACT The aim of this study to measure the fractions of the total serum proteins of the Campeiro horse and identify the influences of biological variants. Blood samples were taken in 138 horses of the breed Campeiro for measuring the concentration of total serum protein by the biuret method. Serum concentrations of protein fractions were measured by electrophoresis using agarose gel. Groups were formed according to age, sex and reproductive condition. The average values of serum fractions: albumin (2.85±0.36g/dl), alpha 1 (0.28±0.11g/dl), alpha 2 (0.26±0.08g/dL) beta 1 (0.57±0.15g/dl), beta 2 (0.89±0.28g/dL), gamaglobulinas (1.86±0.34g/dL), albumin/globulin ratio (0.75±0.18) and 2.5% percentile and 97.5% had slight differences in relation to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Albumin; Clinical patology; Globulins; Serum proteins.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200363

Serum γ-glutamyltransferase activity as an indicator of chronic liver injury in cattle with no clinical signs
Provedor de dados: 45
Autores: Moreira,C.N.; Souza,S.N.; Barini,A.C.; Araújo,E.G.; Fioravanti,M.C.S.
This study aimed to determine the power of the serum aspartate aminotransferase (AST) and gamma-glutamyltransferasase (GGT) activities and of the albumin and cholesterol dosages for detecting hepatic histopathological injuries. A total of 220 healthy male Nelore cattle that had been extensively bred were evaluated. Blood and liver samples were collected on the day of slaughter for biochemical and histopathological tests. The results showed that the sensitivity to AST, GGT, albumin, and cholesterol tests were respectively 22.4%, 22.4%, 36%, and 37.2%. The specificity of AST, GGT, albumin, and cholesterol tests was respectively 78.8%, 90.4%, 75.6%, and 68.3%. In short, the detection of minor liver injuries through biochemical tests is limited; however, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; Albumin; AST; Cholesterol; Clinical biochemistry; GGT.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000600001

Using egg albumin foam to extinguish fires
Provedor de dados: 52
Autores: Alsaati,Hytham A.; Ditzler,Danielle; Burapatana,Vorakan; Tanner,Robert D.
Oil, coal and chemical fires are often difficult to put out using water. In certain hydrocarbon fires, protein foam can extinguish fires better than water by keeping air (oxygen) away from the flames and by ''blowing'' the flame away from its fuel source. Egg albumin is a relatively inexpensive protein and is representative of foaming proteins, which are candidates for use as fire suppression agents. This paper begins to deal with the effect of the foam bulk pH, foam protein concentration and generating air flow rate into the foam on the fire extinguishing time in laboratory experiments. A Bunsen burner was used to generate a small, controlled laboratory fire within a plastic container, which represented a point source in a partially open room in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein Foam; Albumin; Fire Suppression; Biodegradable Foams; Natural Gas Flame.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400029

Radiation-induced Changes in the Electrophoretic Profile of Serum Albumin
Provedor de dados: 52
Autores: Lima,Celso Vieira; Campos,Tarcisio Passos Ribeiro.
ABSTRACT Albumin protein profiles were investigated in electrophoresis system in relation to the whole body exposition to the radiation. Two groups of rats Wistar were set up as the control (CG) and the irradiated one (IG). The IG was exposed to Co-60 at a dose of 5 Gy. After a 72-hour exposition, 300 μL of blood was collected in the inferior vena cava, renal, jugular, hepatic, and pulmonary veins and the serum separated. The albumin protein was identified by vertical electrophoresis in acrylamide Commassi blue or silver stained. The calibration procedure was applied to albumin samples with well-known concentrations. The mathematical correlation was developed involving electrophoretic parameters of band intensities and sizes from gel representation,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serum protein; Rats; Radiation; Albumin; Electrophoresis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100444

Quenching of the intrinsic fluorescence of bovine serum albumin by chlorpromazine and hemin
Provedor de dados: 56
Autores: Silva,D.; Cortez,C.M.; Louro,S.R.W.
The binding of chlorpromazine (CPZ) and hemin to bovine serum albumin was studied by the fluorescence quenching technique. CPZ is a widely used anti-psychotic drug that interacts with blood components, influences bioavailability, and affects function of several biomolecules. Hemin is an important ferric residue of hemoglobin that binds within the hydrophobic region of albumin with high specificity. Quenching of the intrinsic fluorescence of bovine serum albumin (BSA) was observed by selectively exciting tryptophan residues at 290 nm. Emission spectra were recorded in the range from 300 to 450 nm for each quencher addition. Stern-Volmer graphs were plotted, and the quenching constant estimated for BSA solution titrated with hemin at 25ºC was 1.44 (± 0.05) x...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Chlorpromazine; Hemin; Albumin; Fluorescence quenching.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700004

Oxidation of the albumin thiol to sulfenic acid and its implications in the intravascular compartment
Provedor de dados: 56
Autores: Turell,L.; Carballal,S.; Botti,H.; Radi,R.; Alvarez,B.
Human serum albumin (HSA) is the most abundant protein in the intravascular compartment. It possesses a single thiol, Cys34, which constitutes ~80% of the total thiols in plasma. This thiol is able to scavenge plasma oxidants. A central intermediate in this potential antioxidant activity of human serum albumin is sulfenic acid (HSA-SOH). Work from our laboratories has demonstrated the formation of a relatively stable sulfenic acid in albumin through complementary spectrophotometric and mass spectrometric approaches. Recently, we have been able to obtain quantitative data that allowed us to measure the rate constants of sulfenic acid reactions with molecules of analytical and biological interest. Kinetic considerations led us to conclude that the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thiol; Sulfhydryl; Albumin; Sulfenic acid; Mixed disulfides; Plasma.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400001

Synergistic effect of microRNA and albumin-bound nanoparticles for inhibition of glioblastoma cancer cell proliferation
Provedor de dados: 60
Autores: Shariatnasery,Maria; Irani,Shiva; Soleimani,Masoud; Goodarzi,Navid; Dinarvand,Rassoul.
The functional significance of upregulation miR-34a in combination with albumin-bound paclitaxel nanoparticles in U251 glioblastoma cell line has been evaluated. The MTT assay determined that miR-34a and albumin-bound paclitaxel nanoparticles can reduce cell viability, but the combination of both factors has a stronger effect on cell viability. The application of qRT-PCR has demonstrated that the transduction of miR-34a could lead to exogenous upregulation of miR-34a level and downregulation of SURVIVIN. Moreover, treatment of U251 cells with miR-34a and nanoparticles together considerably inhibit SURVIVIN expression compared to miR-34a and nanoparticles alone. Flow cytometry showed that upon miR-34a overexpression cell cycle arrested in G1 phase, while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiR-34a; Glioblastoma; Albumin; Paclitaxel; Nanoparticles; Combination therapy.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100535